Rh6DG424700

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0016991
Physical Location & Seq
Reverse (-)
60568124 .. 60569045
922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG424700.1

Sequence Viewer

Length: 603 bp
ATGAAGAACATGGAGGTGGTACCTAATTTGGCATTGAAGAGGTTGATTCAGCAGTACTGCCATGAGAATGGGATTCCGTTTGGAGATTTCAGTCGTCGGAATCGTGACGTTGGGAGGAGTACAATTGCTGGTAGCTTGGCGGCTGAGGGAGGTATGAAAATGGTGGCGGAGTTTGTCAATGGAAGTGTTGGGACTGGATCGAATAAGGCTGCTTATGAGATTCGGCTTCTTTCAAAAACGAGCATTTTTAATAGGTCTTGTTTGGTCGAAGCTGCAGTGGTGCCGCATTTGTTGAAGCTGTTGGCTTCTGCAGATTCGGAGACTCAAGAGAATGCAGTTGCAGGGTTGTTGAATCTGTCGAAGCATTTGAAAGGGAAAGCTGTGATTGTTGAAAATGGGGGTGTGGATTTGGTTGTGGATGTTCTGAAGGAGGATCTCAAAGTTGAGGCTTGCCAGCATGCTGCTGGTACATTGTTTTATCTTGCTTCGATTGAGGAGTACCGGGTATTGATCGGAGAAAACCCGAGGGCCATATTGGCTTTGAGGGAGCTGATCAAGAATGAAATCTGTTCATCTTCCGGCCACGACCACGCTCAGGATTAA

Protein Analysis

200

Amino Acids

21.61

Weight (kDa)

6.31

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arm PF00514 84 - 120 1.8e-07 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 2 cut(s) 19, 280
AciI CCGC 3 cut(s) 140, 167, 284
AclWI GGATC 2 cut(s) 205, 441
AcoI YGGCCR 1 cut(s) 580
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 5 cut(s) 21, 56, 121, 469, 500
AfiI CCNNNNNNNGG 1 cut(s) 595
AgsI TTSAA 6 cut(s) 37, 234, 295, 352, 370, 392
AluBI AGCT 5 cut(s) 135, 272, 298, 380, 550
AluI AGCT 5 cut(s) 135, 272, 298, 380, 550
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 2 cut(s) 205, 441
Ama87I CYCGRG 1 cut(s) 523
AoxI GGCC 2 cut(s) 528, 580
ApeKI GCWGC 3 cut(s) 209, 272, 461
Asp718I GGTACC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 528
AsuC2I CCSGG 1 cut(s) 503
AvaI CYCGRG 1 cut(s) 523
BanI GGYRCC 2 cut(s) 19, 280
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 196, 259, 448
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 1 cut(s) 314
BfmI CTRYAG 2 cut(s) 273, 309
BglI GCCNNNNNGGC 1 cut(s) 536
BisI GCNGC 5 cut(s) 141, 210, 273, 284, 462
BlsI GCNGC 5 cut(s) 142, 211, 274, 285, 463
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 523
BmgT120I GGNCC 1 cut(s) 528
BmiI GGNNCC 2 cut(s) 21, 282
BmrFI CCNGG 1 cut(s) 503
Bpu10I CCTNAGC 2 cut(s) 144, 594
BpuEI CTTGAG 1 cut(s) 309
BpuMI CCSGG 1 cut(s) 503
BsaJI CCNNGG 1 cut(s) 524
Bsc4I CCNNNNNNNGG 1 cut(s) 595
Bse1I ACTGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 524
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 1 cut(s) 595
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 2 cut(s) 130, 509
BseXI GCAGC 3 cut(s) 196, 259, 448
BshFI GGCC 2 cut(s) 530, 582
BshNI GGYRCC 2 cut(s) 19, 280
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 2 cut(s) 502, 579
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 595
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 205
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 2 cut(s) 530, 582
BsoBI CYCGRG 1 cut(s) 523
Bsp143I GATC 4 cut(s) 197, 433, 510, 552
BspACI CCGC 3 cut(s) 140, 167, 284
BspANI GGCC 2 cut(s) 530, 582
BspCNI CTCAG 1 cut(s) 136
BspLI GGNNCC 2 cut(s) 21, 282
BspMAI CTGCAG 2 cut(s) 277, 313
BspPI GGATC 2 cut(s) 205, 441
BspT107I GGYRCC 2 cut(s) 19, 280
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 524
BssMI GATC 4 cut(s) 197, 433, 510, 552
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 3 cut(s) 451, 455, 459
BstDEI CTNAG 2 cut(s) 144, 594
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 4 cut(s) 200, 436, 513, 555
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 4 cut(s) 197, 433, 510, 552
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNSI RCATGY 1 cut(s) 461
BstSCI CCNGG 1 cut(s) 501
BstSFI CTRYAG 2 cut(s) 273, 309
BstV1I GCAGC 3 cut(s) 196, 259, 448
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 68
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 2 cut(s) 530, 582
BtsCI GGATG 1 cut(s) 424
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 3 cut(s) 451, 455, 459
Cfr13I GGNCC 1 cut(s) 528
Csp6I GTAC 5 cut(s) 20, 55, 120, 468, 499
CviAII CATG 3 cut(s) 10, 62, 458
CviQI GTAC 5 cut(s) 20, 55, 120, 468, 499
DdeI CTNAG 2 cut(s) 144, 594
DpnI GATC 4 cut(s) 199, 435, 512, 554
DpnII GATC 4 cut(s) 197, 433, 510, 552
EaeI YGGCCR 1 cut(s) 580
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 523
FaeI CATG 3 cut(s) 13, 65, 461
FaiI YATR 6 cut(s) 11, 63, 155, 216, 459, 533
FaqI GGGAC 1 cut(s) 205
FatI CATG 3 cut(s) 9, 61, 457
FbaI TGATCA 1 cut(s) 552
Fnu4HI GCNGC 5 cut(s) 141, 210, 273, 284, 462
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 5 cut(s) 141, 210, 273, 284, 462
GluI GCNGC 5 cut(s) 141, 210, 273, 284, 462
HaeIII GGCC 2 cut(s) 530, 582
HapII CCGG 2 cut(s) 502, 579
Hin1II CATG 3 cut(s) 13, 65, 461
HinfI GANTC 7 cut(s) 46, 73, 100, 220, 314, 322, 352
HpaII CCGG 2 cut(s) 502, 579
Hpy188I TCNGA 4 cut(s) 99, 319, 426, 515
Hpy188III TCNNGA 4 cut(s) 104, 326, 556, 596
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 1 cut(s) 421
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 4 cut(s) 275, 311, 335, 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 2 cut(s) 144, 594
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 3 cut(s) 13, 65, 461
KpnI GGTACC 1 cut(s) 23
Ksp22I TGATCA 1 cut(s) 552
Kzo9I GATC 4 cut(s) 197, 433, 510, 552
LmnI GCTCC 1 cut(s) 547
LpnPI CCDG 8 cut(s) 114, 180, 327, 450, 467, 515, 581, 592
Lsp1109I GCAGC 3 cut(s) 196, 259, 448
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 104
MalI GATC 4 cut(s) 199, 435, 512, 554
MboI GATC 4 cut(s) 197, 433, 510, 552
MboII GAAGA 3 cut(s) 16, 49, 567
MfeI CAATTG 1 cut(s) 123
MflI RGATCY 1 cut(s) 433
MluCI AATT 2 cut(s) 25, 123
MlyI GAGTC 1 cut(s) 316
MmeI TCCRAC 1 cut(s) 77
MseI TTAA 2 cut(s) 249, 601
MslI CAYNNNNRTG 2 cut(s) 14, 66
MspI CCGG 2 cut(s) 502, 579
MspR9I CCNGG 1 cut(s) 503
MunI CAATTG 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 1 cut(s) 503
NdeII GATC 4 cut(s) 197, 433, 510, 552
NlaIII CATG 3 cut(s) 13, 65, 461
NlaIV GGNNCC 2 cut(s) 21, 282
NmuCI GTSAC 1 cut(s) 104
NspI RCATGY 1 cut(s) 461
PaeI GCATGC 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 100
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 6 cut(s) 46, 73, 100, 220, 314, 352
PkrI GCNGC 5 cut(s) 142, 211, 274, 285, 463
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 21, 282
PspPI GGNCC 1 cut(s) 528
PstI CTGCAG 2 cut(s) 277, 313
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 5 cut(s) 21, 56, 121, 469, 500
RsaNI GTAC 5 cut(s) 20, 55, 120, 468, 499
RseI CAYNNNNRTG 2 cut(s) 14, 66
SaqAI TTAA 2 cut(s) 249, 601
SatI GCNGC 5 cut(s) 141, 210, 273, 284, 462
Sau3AI GATC 4 cut(s) 197, 433, 510, 552
Sau96I GGNCC 1 cut(s) 528
ScaI AGTACT 1 cut(s) 56
SchI GAGTC 1 cut(s) 316
ScrFI CCNGG 1 cut(s) 503
SfcI CTRYAG 2 cut(s) 273, 309
SmiMI CAYNNNNRTG 2 cut(s) 14, 66
SmlI CTYRAG 1 cut(s) 324
SmoI CTYRAG 1 cut(s) 324
SphI GCATGC 1 cut(s) 461
Sse9I AATT 2 cut(s) 25, 123
SsiI CCGC 3 cut(s) 140, 167, 284
StyD4I CCNGG 1 cut(s) 501
TaiI ACGT 1 cut(s) 111
TaqI TCGA 4 cut(s) 200, 267, 359, 488
TasI AATT 2 cut(s) 25, 123
TatI WGTACW 2 cut(s) 54, 119
TauI GCSGC 2 cut(s) 143, 286
TfiI GAWTC 6 cut(s) 46, 73, 100, 220, 314, 352
Tru1I TTAA 2 cut(s) 249, 601
Tru9I TTAA 2 cut(s) 249, 601
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 3 cut(s) 209, 272, 461
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 4 cut(s) 17, 170, 561, 576
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 1 cut(s) 282
XceI RCATGY 1 cut(s) 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 461
ZrmI AGTACT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.