Rh7BG269400

arbuscular mycorrhizal association

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006933
Physical Location & Seq
Forward (+)
24714404 .. 24719354
4951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG269400.1

Sequence Viewer

Length: 1464 bp
ATGGAAGGAAGGGGGCTTTCAGACTTCTATAGGAATACTAGTGAAGAGTTGATCCTGAGATCTTATATGGAGAGCTCAAATGGAACTCCTCTACCAACCATGGAGATGCTTGGCTTCAAGAATTTGTCACAAAATTTTCGTGCAGATAGCGAGGAGCTCTTCAAAAGCTGGCTCACAACTGGAGAGAACAATACCTACAATTCCTCAAGCATTGCACATCGAACTCGGTCAAGGAGGATATCCACCGAAATAGCTAGTTTGTCTGCTCACCAACATGTTGGTCTACTTCAAAAGAAAAGAAGCAATGAAGTTTTATTTCCTCAATATAACCCCATGCCTGATGAGAACTCAGCTGACCTCAATCAACATTCAAACAGGAAGAGATATGTTGCCTTGCAAAATTCTCAACCAGCAATAGGTCTGGATGGCCTTCAAAATGCTTCAGGGAATTATAACAATGTAGTAAAAGAAGAATTTGCATTTTCAAATGGCTTTAATGTTCCCTCAATATGTGAGGTTTCTAACCAGTTGGGGACCTTCATTTCTCCATCCAATTCATCCTCTTCCACCTTCGACGCTCCTCAAATGGGTGACATGGATAAAGTATCATCTGTTGTGAGCATGCTAAAGGGTACATTAGAGCGCAAAAAGCTTAGCAACCAGATTGAAAAAGGTGCAGAGGATGATAGCTCCACTAGGCTTTTCCCTGGTCAAGAAGTTATAGTCAATACTGGTTTTGATCAAGAGCAAGGTAATCAGCTTCAAGATATGGTGAGAACTTTTCAGGAAGTATCCACTATAGAAGTTAAGGATCATGCGGGTATGCAAAAAGCTGAAGGATCCCTGGATCTTGAGATGGAAGGTTTTGTAAATCTCACAAACCCTAACCCATTGAGCAGGAATTCTCAAGAACCTTCACAAAGTGAATCATCTGCTGCAGCACCAGTAGTTTCATCTGGGTTTGATGCATGTGATGGTCCCAGCAACTCAAGTCAAACTCTGAGCATATGTGAAAGTTCACTGAAACGAGCTGGAAATAGGAGTTCAGAAAATGGCTCTAGATCGAAAGATATCAGAGAACGTATTATTGGTAATTTGAAAGATGATCAAAAGAGGGGAGAACGTTTAGAGCGATATGGATCTGTGACATCAGCCACTTCAGGAGATAAGGAGGATGCCACAAAAAAGCGGAGGGTGGAGAGATCACGCAAAATGGCAGAGGCAAAGGAAAGGAACTCAACTCCAGTAATTCCATCAGATATACAATCTGTCTTAAAGCGGTGTGAAAATCTTGAAAAGGAAGTCCGGTCCCTCAAACTTAACTTGTCCTTCATGAATAGGAAGGATTCCGAGCAGACAAAGCAGATAGAGGAGCTTCAGCAGCAAAACGAAGAATTAACAGATGAAAAAGAGCGCCTTTTAGAAGAGATTGAAAGGATCCTTGCAGAAAATGGCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0036377 GO:0044403 GO:0044419 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

487

Amino Acids

54.38

Weight (kDa)

5.33

Isoelectric Point (pI)

59.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
AccI GTMKAC 1 cut(s) 283
AciI CCGC 3 cut(s) 818, 1189, 1279
AclI AACGTT 1 cut(s) 1123
AclWI GGATC 8 cut(s) 46, 819, 834, 847, 855, 1147, 1432, 1445
AcsI RAATTY 5 cut(s) 121, 133, 400, 473, 901
AcuI CTGAAG 4 cut(s) 426, 855, 1143, 1361
AdeI CACNNNGTG 1 cut(s) 923
AfaI GTAC 1 cut(s) 634
AfiI CCNNNNNNNGG 2 cut(s) 416, 587
AflIII ACRYGT 1 cut(s) 274
AhlI ACTAGT 1 cut(s) 38
AjnI CCWGG 2 cut(s) 706, 843
AjuI GAANNNNNNNTTGG 2 cut(s) 1071, 1103
Alw21I GWGCWC 2 cut(s) 77, 159
AlwI GGATC 8 cut(s) 46, 819, 834, 847, 855, 1147, 1432, 1445
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 3 cut(s) 935, 938, 1381
ApoI RAATTY 5 cut(s) 121, 133, 400, 473, 901
AspLEI GCGC 2 cut(s) 645, 1416
AspS9I GGNCC 3 cut(s) 534, 977, 1308
AsuHPI GGTGA 3 cut(s) 260, 602, 784
AvaII GGWCC 3 cut(s) 534, 977, 1308
BamHI GGATCC 2 cut(s) 839, 1437
BanII GRGCYC 2 cut(s) 77, 159
Bbv12I GWGCWC 2 cut(s) 77, 159
BbvI GCAGC 3 cut(s) 922, 950, 1393
BccI CCATC 5 cut(s) 419, 556, 850, 968, 1261
BciT130I CCWGG 2 cut(s) 708, 845
BciVI GTATCC 1 cut(s) 802
BclI TGATCA 2 cut(s) 739, 1105
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 4 cut(s) 39, 255, 696, 1059
BfmI CTRYAG 3 cut(s) 28, 798, 936
BfoI RGCGCY 1 cut(s) 1417
BfuI GTATCC 1 cut(s) 802
BglII AGATCT 1 cut(s) 59
BisI GCNGC 3 cut(s) 936, 939, 1382
BlpI GCTNAGC 1 cut(s) 653
BlsI GCNGC 3 cut(s) 937, 940, 1383
Bme1390I CCNGG 2 cut(s) 708, 845
Bme18I GGWCC 3 cut(s) 534, 977, 1308
BmgT120I GGNCC 3 cut(s) 534, 977, 1308
BmiI GGNNCC 5 cut(s) 535, 841, 979, 1310, 1439
BmrFI CCNGG 2 cut(s) 708, 845
BmsI GCATC 3 cut(s) 96, 955, 1165
BpmI CTGGAG 2 cut(s) 201, 1227
Bpu1102I GCTNAGC 1 cut(s) 653
BpuEI CTTGAG 4 cut(s) 190, 872, 891, 973
BsaBI GATNNNNATC 1 cut(s) 1138
BsaJI CCNNGG 3 cut(s) 99, 706, 843
BsaWI WCCGGW 1 cut(s) 1305
Bsc4I CCNNNNNNNGG 2 cut(s) 416, 587
Bse1I ACTGG 5 cut(s) 184, 526, 736, 944, 1244
Bse3DI GCAATG 2 cut(s) 210, 310
Bse8I GATNNNNATC 1 cut(s) 1138
BseBI CCWGG 2 cut(s) 708, 845
BseDI CCNNGG 3 cut(s) 99, 706, 843
BseGI GGATG 5 cut(s) 430, 548, 557, 688, 1180
BseJI GATNNNNATC 1 cut(s) 1138
BseLI CCNNNNNNNGG 2 cut(s) 416, 587
BseMI GCAATG 2 cut(s) 210, 310
BseMII CTCAG 3 cut(s) 47, 363, 992
BseNI ACTGG 5 cut(s) 184, 526, 736, 944, 1244
BseRI GAGGAG 4 cut(s) 78, 167, 570, 1385
BseXI GCAGC 3 cut(s) 922, 950, 1393
BseYI CCCAGC 1 cut(s) 980
BsgI GTGCAG 2 cut(s) 162, 696
BshFI GGCC 1 cut(s) 429
BsiHKAI GWGCWC 2 cut(s) 77, 159
BsiSI CCGG 1 cut(s) 1306
BslFI GGGAC 3 cut(s) 547, 963, 1294
BslI CCNNNNNNNGG 2 cut(s) 416, 587
BsmFI GGGAC 3 cut(s) 547, 963, 1294
BsnI GGCC 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 77, 159
Bsp1720I GCTNAGC 1 cut(s) 653
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 3 cut(s) 818, 1189, 1279
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 3 cut(s) 48, 362, 993
BspHI TCATGA 1 cut(s) 1332
BspLI GGNNCC 5 cut(s) 535, 841, 979, 1310, 1439
BspMAI CTGCAG 1 cut(s) 940
BspPI GGATC 8 cut(s) 46, 819, 834, 847, 855, 1147, 1432, 1445
BspQI GCTCTTC 1 cut(s) 164
BsrDI GCAATG 2 cut(s) 210, 310
BsrI ACTGG 5 cut(s) 184, 526, 736, 944, 1244
BssECI CCNNGG 3 cut(s) 99, 706, 843
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 708, 845
Bst6I CTCTTC 5 cut(s) 39, 164, 374, 568, 1419
BstC8I GCNNGC 2 cut(s) 170, 623
BstDEI CTNAG 4 cut(s) 56, 349, 653, 1001
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 5 cut(s) 430, 548, 557, 688, 1180
BstH2I RGCGCY 1 cut(s) 1417
BstHHI GCGC 2 cut(s) 645, 1416
BstMWI GCNNNNNNNGC 3 cut(s) 649, 1360, 1381
BstNI CCWGG 2 cut(s) 708, 845
BstNSI RCATGY 3 cut(s) 278, 625, 972
BstSCI CCNGG 2 cut(s) 706, 843
BstSFI CTRYAG 3 cut(s) 28, 798, 936
BstV1I GCAGC 3 cut(s) 922, 950, 1393
BstX2I RGATCY 5 cut(s) 59, 839, 847, 1139, 1437
BstXI CCANNNNNNTGG 1 cut(s) 278
BstYI RGATCY 5 cut(s) 59, 839, 847, 1139, 1437
BsuI GTATCC 1 cut(s) 802
BsuRI GGCC 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 5 cut(s) 430, 548, 557, 688, 1180
BtsIMutI CAGTG 1 cut(s) 1019
Cac8I GCNNGC 2 cut(s) 170, 623
CciI TCATGA 1 cut(s) 1332
CfoI GCGC 2 cut(s) 645, 1416
Cfr13I GGNCC 3 cut(s) 534, 977, 1308
CseI GACGC 1 cut(s) 584
Csp6I GTAC 1 cut(s) 633
CviAII CATG 8 cut(s) 100, 275, 334, 595, 622, 815, 969, 1333
CviQI GTAC 1 cut(s) 633
DdeI CTNAG 4 cut(s) 56, 349, 653, 1001
DraIII CACNNNGTG 1 cut(s) 923
Eam1104I CTCTTC 5 cut(s) 39, 164, 374, 568, 1419
EarI CTCTTC 5 cut(s) 39, 164, 374, 568, 1419
Ecl136II GAGCTC 2 cut(s) 75, 157
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 77, 159
Eco32I GATATC 2 cut(s) 240, 1072
Eco47I GGWCC 3 cut(s) 534, 977, 1308
Eco53kI GAGCTC 2 cut(s) 75, 157
Eco57I CTGAAG 4 cut(s) 426, 855, 1143, 1361
EcoICRI GAGCTC 2 cut(s) 75, 157
EcoO109I RGGNCCY 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 901
EcoRII CCWGG 2 cut(s) 706, 843
EcoRV GATATC 2 cut(s) 240, 1072
EcoT14I CCWWGG 1 cut(s) 99
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 2 cut(s) 77, 159
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 8 cut(s) 103, 278, 337, 598, 625, 818, 972, 1336
FalI AAGNNNNNCTT 2 cut(s) 1401, 1433
FaqI GGGAC 3 cut(s) 547, 963, 1294
FatI CATG 8 cut(s) 99, 274, 333, 594, 621, 814, 968, 1332
FauI CCCGC 1 cut(s) 811
FauNDI CATATG 1 cut(s) 1007
FbaI TGATCA 2 cut(s) 739, 1105
FblI GTMKAC 1 cut(s) 283
Fnu4HI GCNGC 3 cut(s) 936, 939, 1382
FokI GGATG 5 cut(s) 437, 535, 544, 695, 1187
FriOI GRGCYC 2 cut(s) 77, 159
Fsp4HI GCNGC 3 cut(s) 936, 939, 1382
FspBI CTAG 4 cut(s) 39, 255, 696, 1059
GlaI GCGC 2 cut(s) 644, 1415
GluI GCNGC 3 cut(s) 936, 939, 1382
GsaI CCCAGC 1 cut(s) 984
GsuI CTGGAG 2 cut(s) 201, 1227
HaeII RGCGCY 1 cut(s) 1417
HaeIII GGCC 1 cut(s) 429
HapII CCGG 1 cut(s) 1306
HgaI GACGC 1 cut(s) 584
HhaI GCGC 2 cut(s) 645, 1416
Hin1II CATG 8 cut(s) 103, 278, 337, 598, 625, 818, 972, 1336
Hin6I GCGC 2 cut(s) 643, 1414
HinP1I GCGC 2 cut(s) 643, 1414
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 2 cut(s) 926, 1346
HpaII CCGG 1 cut(s) 1306
HphI GGTGA 3 cut(s) 260, 602, 784
Hpy166II GTNNAC 2 cut(s) 284, 1019
Hpy188I TCNGA 6 cut(s) 22, 1002, 1048, 1076, 1258, 1351
Hpy8I GTNNAC 2 cut(s) 284, 1019
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 9 cut(s) 3, 440, 547, 580, 830, 854, 924, 1336, 1339
HpyCH4IV ACGT 2 cut(s) 1081, 1123
HpyCH4V TGCA 9 cut(s) 143, 215, 397, 479, 677, 826, 938, 968, 1445
HpyF10VI GCNNNNNNNGC 3 cut(s) 649, 1360, 1381
HpyF3I CTNAG 4 cut(s) 56, 349, 653, 1001
HpySE526I ACGT 2 cut(s) 1081, 1123
Hsp92II CATG 8 cut(s) 103, 278, 337, 598, 625, 818, 972, 1336
HspAI GCGC 2 cut(s) 643, 1414
Ksp22I TGATCA 2 cut(s) 739, 1105
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 4 cut(s) 154, 583, 695, 1372
Lsp1109I GCAGC 3 cut(s) 922, 950, 1393
LweI GCATC 3 cut(s) 96, 955, 1165
MaeI CTAG 4 cut(s) 39, 255, 696, 1059
MaeII ACGT 2 cut(s) 1081, 1123
MaeIII GTNAC 3 cut(s) 126, 590, 1144
MboII GAAGA 7 cut(s) 56, 151, 391, 482, 555, 1403, 1436
MflI RGATCY 5 cut(s) 59, 839, 847, 1139, 1437
MhlI GDGCHC 2 cut(s) 77, 159
Mph1103I ATGCAT 1 cut(s) 970
MseI TTAA 5 cut(s) 495, 807, 1274, 1320, 1397
MslI CAYNNNNRTG 2 cut(s) 104, 273
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 1 cut(s) 1306
MspR9I CCNGG 2 cut(s) 708, 845
MvaI CCWGG 2 cut(s) 708, 845
MwoI GCNNNNNNNGC 3 cut(s) 649, 1360, 1381
NcoI CCATGG 1 cut(s) 99
NdeI CATATG 1 cut(s) 1007
NlaIII CATG 8 cut(s) 103, 278, 337, 598, 625, 818, 972, 1336
NlaIV GGNNCC 5 cut(s) 535, 841, 979, 1310, 1439
NmuCI GTSAC 3 cut(s) 126, 590, 1144
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 3 cut(s) 278, 625, 972
PaeI GCATGC 1 cut(s) 625
PagI TCATGA 1 cut(s) 1332
PciI ACATGT 1 cut(s) 274
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 1 cut(s) 1129
PfeI GAWTC 2 cut(s) 926, 1346
PkrI GCNGC 3 cut(s) 937, 940, 1383
PpuMI RGGWCCY 1 cut(s) 534
PscI ACATGT 1 cut(s) 274
PsiI TTATAA 1 cut(s) 453
Psp124BI GAGCTC 2 cut(s) 77, 159
Psp1406I AACGTT 1 cut(s) 1123
Psp5II RGGWCCY 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 706, 843
PspFI CCCAGC 1 cut(s) 980
PspGI CCWGG 2 cut(s) 706, 843
PspN4I GGNNCC 5 cut(s) 535, 841, 979, 1310, 1439
PspPI GGNCC 3 cut(s) 534, 977, 1308
PspPPI RGGWCCY 1 cut(s) 534
PsrI GAACNNNNNNTAC 2 cut(s) 179, 211
PstI CTGCAG 1 cut(s) 940
PsuI RGATCY 5 cut(s) 59, 839, 847, 1139, 1437
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 634
RsaNI GTAC 1 cut(s) 633
RseI CAYNNNNRTG 2 cut(s) 104, 273
SacI GAGCTC 2 cut(s) 77, 159
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 5 cut(s) 495, 807, 1274, 1320, 1397
SatI GCNGC 3 cut(s) 936, 939, 1382
Sau96I GGNCC 3 cut(s) 534, 977, 1308
ScrFI CCNGG 2 cut(s) 708, 845
SduI GDGCHC 2 cut(s) 77, 159
SfaNI GCATC 3 cut(s) 96, 955, 1165
SfcI CTRYAG 3 cut(s) 28, 798, 936
SinI GGWCC 3 cut(s) 534, 977, 1308
SmiMI CAYNNNNRTG 2 cut(s) 104, 273
SmlI CTYRAG 4 cut(s) 205, 851, 906, 988
SmoI CTYRAG 4 cut(s) 205, 851, 906, 988
SpeI ACTAGT 1 cut(s) 38
SphI GCATGC 1 cut(s) 625
SsiI CCGC 3 cut(s) 818, 1189, 1279
SspMI CTAG 4 cut(s) 39, 255, 696, 1059
SstI GAGCTC 2 cut(s) 77, 159
StyD4I CCNGG 2 cut(s) 706, 843
StyI CCWWGG 1 cut(s) 99
TaiI ACGT 2 cut(s) 1084, 1126
TaqI TCGA 3 cut(s) 220, 573, 1064
TaqII GACCGA 1 cut(s) 216
TfiI GAWTC 2 cut(s) 926, 1346
Tru1I TTAA 5 cut(s) 495, 807, 1274, 1320, 1397
Tru9I TTAA 5 cut(s) 495, 807, 1274, 1320, 1397
TscAI CASTG 1 cut(s) 1026
TseFI GTSAC 3 cut(s) 126, 590, 1144
TseI GCWGC 3 cut(s) 935, 938, 1381
Tsp45I GTSAC 3 cut(s) 126, 590, 1144
TspDTI ATGAA 7 cut(s) 321, 529, 546, 942, 1321, 1349, 1419
TspRI CASTG 1 cut(s) 1026
VpaK11BI GGWCC 3 cut(s) 534, 977, 1308
XapI RAATTY 5 cut(s) 121, 133, 400, 473, 901
XbaI TCTAGA 1 cut(s) 1058
XceI RCATGY 3 cut(s) 278, 625, 972
XmiI GTMKAC 1 cut(s) 283
XspI CTAG 4 cut(s) 39, 255, 696, 1059
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.