Rw0G008920

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00346
OG0016460
Physical Location & Seq
Reverse (-)
28799 .. 33572
4774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008920.1

Sequence Viewer

Length: 1128 bp
ATGTTAGCTCATCAGGAAGTACTCTTGGATCCTACTTTACTTGGGCCTACTCCAATGGACCCACCACCCAATTTACCCATATTTCCACTTCCTCCTTTTTTAGACCCTTATACTCGACACTCATATTCTCTTAACCCCTATCCTCTCCATATCCCTTCTCTCCAGCCGTCACCTCCAATAATCCCACCAGTCCCACTCTCAACAAATATCCACCCTGAAGTTTTTGCTGAAATTCCACCAACAATCCCTCCGTTTGGGTCTCTTGCTATACTTGCTCAGCAATTCCAGTTCCTCAAATTACTTAAAAGGCTGCAAGATGATTTGGCTGAGGATTTTCATCAAACCAAGCACCACCGCACTATGGATTCCGATGATAGTACTCGGGTGGATCAGGCTATTCCCTCTCGAGTCCCTACTAAACCACGTCGTGGGCATCCTCCGTATGCTACTGCCTCTGGTAAGGCCAAGGGGTTGGCTCGACGTGGTCTTCCAAAGAAGATTCAAACTTGTAATAACCCTACTGCTCCGGCTACTGTTGAAATGGTTAGTGAGGTTCAGATTGCTTCATCAGTCTTATCCGATGCAACACCTTATCTTCCTGTGATGCAAGACCTAAGTTTGAATGTTGTGGTTCAACCTTCATCTGAGGTGGCTTCTTCGGAGTTTCTTCGAGTTGTGATAAAGACTAAGTGCCCCCAAGCTTCTAGTTTGCCCTCCAGGACTCCTCCAAAGCCAAAATGGTTACCACCGCCTCCAAACTATACTAAGATAAATTCTGATGCATCCTGGTCTTCTACTGAGGTTTGTTTGGCGGCTATTGCTCGAAACTGTGATGGAGTTGTGGTTGGTGGAAGTCATCTTTCTACTTCAGCTCCATCGCCTCTGTATGCAGAAGCAGTGGCCCTCTGCTTGATTGTTCATCTTGCTCAAAAGCTTCAAGCAGATAAATTCCTCTTCGAATCGGATTCACTTCAACTTGTCAATCTCCTTCAACATTCTTCTCCATCTTCAGATTGGGTTGTTATGCCCCTGATTGATCATATAAGAGATTTGACAGTTGCTATTCGTTCCTACAGCTGGACTTGGTCCAGCAGATTGGCAAATCAGGCTGCAGACCATGTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

375

Amino Acids

41.02

Weight (kDa)

6.89

Isoelectric Point (pI)

58.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 258 - 375 3.4e-14 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 428
AciI CCGC 3 cut(s) 355, 749, 812
AclWI GGATC 3 cut(s) 23, 36, 396
AcsI RAATTY 3 cut(s) 231, 772, 947
AcuI CTGAAG 3 cut(s) 237, 852, 993
AdeI CACNNNGTG 1 cut(s) 428
AfaI GTAC 2 cut(s) 21, 379
AfiI CCNNNNNNNGG 4 cut(s) 254, 361, 428, 1077
AgsI TTSAA 7 cut(s) 503, 539, 622, 635, 938, 974, 992
AjiI CACGTC 2 cut(s) 425, 482
AjnI CCWGG 2 cut(s) 716, 785
AluBI AGCT 5 cut(s) 8, 701, 872, 934, 1077
AluI AGCT 5 cut(s) 8, 701, 872, 934, 1077
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 3 cut(s) 23, 36, 396
Ama87I CYCGRG 2 cut(s) 381, 405
AoxI GGCC 3 cut(s) 44, 462, 900
ApeKI GCWGC 2 cut(s) 310, 1109
ApoI RAATTY 3 cut(s) 231, 772, 947
AspS9I GGNCC 4 cut(s) 44, 58, 901, 1086
AsuHPI GGTGA 1 cut(s) 162
AsuII TTCGAA 1 cut(s) 957
AvaI CYCGRG 2 cut(s) 381, 405
AvaII GGWCC 2 cut(s) 58, 1086
BaeGI GKGCMC 1 cut(s) 695
BamHI GGATCC 1 cut(s) 28
BbsI GAAGAC 2 cut(s) 479, 783
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 297, 1096
BccI CCATC 3 cut(s) 827, 883, 1012
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 2 cut(s) 718, 787
BclI TGATCA 1 cut(s) 1036
BcoDI GTCTC 1 cut(s) 264
BfaI CTAG 2 cut(s) 705, 1126
BfmI CTRYAG 2 cut(s) 1072, 1110
BisI GCNGC 3 cut(s) 311, 813, 1110
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 3 cut(s) 312, 814, 1111
BmcAI AGTACT 2 cut(s) 21, 379
Bme1390I CCNGG 2 cut(s) 718, 787
Bme18I GGWCC 2 cut(s) 58, 1086
BmeT110I CYCGRG 2 cut(s) 381, 405
BmgBI CACGTC 2 cut(s) 425, 482
BmgT120I GGNCC 4 cut(s) 44, 58, 901, 1086
BmiI GGNNCC 2 cut(s) 30, 60
BmrFI CCNGG 2 cut(s) 718, 787
BmsI GCATC 5 cut(s) 442, 571, 594, 769, 791
BpiI GAAGAC 2 cut(s) 479, 783
BpmI CTGGAG 2 cut(s) 146, 700
Bpu10I CCTNAGC 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 957
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 465
BsaXI ACNNNNNCTCC 6 cut(s) 232, 262, 828, 856, 858, 886
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 361, 428, 1077
Bse1I ACTGG 2 cut(s) 188, 286
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 2 cut(s) 718, 787
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 2 cut(s) 433, 782
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 4 cut(s) 254, 361, 428, 1077
BseMII CTCAG 4 cut(s) 290, 318, 636, 789
BseNI ACTGG 2 cut(s) 188, 286
BseRI GAGGAG 1 cut(s) 714
BseSI GKGCMC 1 cut(s) 695
BseXI GCAGC 2 cut(s) 297, 1096
BshFI GGCC 3 cut(s) 46, 464, 902
BsiHKCI CYCGRG 2 cut(s) 381, 405
BsiSI CCGG 1 cut(s) 527
BslFI GGGAC 2 cut(s) 176, 395
BslI CCNNNNNNNGG 4 cut(s) 254, 361, 428, 1077
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 2 cut(s) 176, 395
BsnI GGCC 3 cut(s) 46, 464, 902
Bso31I GGTCTC 1 cut(s) 264
BsoBI CYCGRG 2 cut(s) 381, 405
Bsp119I TTCGAA 1 cut(s) 957
Bsp1286I GDGCHC 1 cut(s) 695
Bsp143I GATC 3 cut(s) 28, 388, 1036
Bsp1720I GCTNAGC 1 cut(s) 276
BspACI CCGC 3 cut(s) 355, 749, 812
BspANI GGCC 3 cut(s) 46, 464, 902
BspCNI CTCAG 4 cut(s) 289, 319, 637, 790
BspLI GGNNCC 2 cut(s) 30, 60
BspMAI CTGCAG 1 cut(s) 1114
BspPI GGATC 3 cut(s) 23, 36, 396
BspT104I TTCGAA 1 cut(s) 957
BspTNI GGTCTC 1 cut(s) 264
BsrI ACTGG 2 cut(s) 188, 286
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 3 cut(s) 28, 388, 1036
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 718, 787
Bst4CI ACNGT 3 cut(s) 535, 830, 1057
Bst6I CTCTTC 1 cut(s) 959
BstBI TTCGAA 1 cut(s) 957
BstDEI CTNAG 7 cut(s) 276, 327, 614, 645, 687, 765, 798
BstEII GGTNACC 1 cut(s) 741
BstF5I GGATG 2 cut(s) 433, 782
BstKTI GATC 3 cut(s) 31, 391, 1039
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 3 cut(s) 28, 388, 1036
BstMWI GCNNNNNNNGC 3 cut(s) 272, 818, 1106
BstNI CCWGG 2 cut(s) 718, 787
BstPI GGTNACC 1 cut(s) 741
BstSCI CCNGG 2 cut(s) 716, 785
BstSFI CTRYAG 2 cut(s) 1072, 1110
BstSLI GKGCMC 1 cut(s) 695
BstV1I GCAGC 2 cut(s) 297, 1096
BstV2I GAAGAC 2 cut(s) 479, 783
BstX2I RGATCY 1 cut(s) 28
BstXI CCANNNNNNTGG 2 cut(s) 472, 1096
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 3 cut(s) 46, 464, 902
BtgZI GCGATG 1 cut(s) 861
BtrI CACGTC 2 cut(s) 425, 482
BtsCI GGATG 2 cut(s) 433, 782
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 1 cut(s) 903
Cfr13I GGNCC 4 cut(s) 44, 58, 901, 1086
Csp6I GTAC 2 cut(s) 20, 378
CviAII CATG 1 cut(s) 1118
CviQI GTAC 2 cut(s) 20, 378
DdeI CTNAG 7 cut(s) 276, 327, 614, 645, 687, 765, 798
DpnI GATC 3 cut(s) 30, 390, 1038
DpnII GATC 3 cut(s) 28, 388, 1036
DraIII CACNNNGTG 1 cut(s) 428
Eam1104I CTCTTC 1 cut(s) 959
EarI CTCTTC 1 cut(s) 959
Eco130I CCWWGG 1 cut(s) 465
Eco31I GGTCTC 1 cut(s) 264
Eco47I GGWCC 2 cut(s) 58, 1086
Eco57I CTGAAG 3 cut(s) 237, 852, 993
Eco88I CYCGRG 2 cut(s) 381, 405
Eco91I GGTNACC 1 cut(s) 741
EcoO65I GGTNACC 1 cut(s) 741
EcoRII CCWGG 2 cut(s) 716, 785
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 784
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 1 cut(s) 1121
FaqI GGGAC 2 cut(s) 176, 395
FatI CATG 1 cut(s) 1117
FbaI TGATCA 1 cut(s) 1036
Fnu4HI GCNGC 3 cut(s) 311, 813, 1110
FokI GGATG 2 cut(s) 420, 769
Fsp4HI GCNGC 3 cut(s) 311, 813, 1110
FspBI CTAG 2 cut(s) 705, 1126
GluI GCNGC 3 cut(s) 311, 813, 1110
GsuI CTGGAG 2 cut(s) 146, 700
HaeIII GGCC 3 cut(s) 46, 464, 902
HapII CCGG 1 cut(s) 527
Hin1II CATG 1 cut(s) 1121
HindIII AAGCTT 2 cut(s) 699, 932
HinfI GANTC 6 cut(s) 365, 408, 499, 721, 959, 965
HpaII CCGG 1 cut(s) 527
HphI GGTGA 1 cut(s) 162
Hpy188I TCNGA 8 cut(s) 370, 558, 580, 646, 661, 778, 964, 1012
Hpy188III TCNNGA 2 cut(s) 14, 405
Hpy99I CGWCG 2 cut(s) 429, 483
HpyAV CCTTC 3 cut(s) 165, 648, 998
HpyCH4III ACNGT 3 cut(s) 535, 830, 1057
HpyCH4IV ACGT 2 cut(s) 424, 481
HpyCH4V TGCA 6 cut(s) 313, 584, 607, 782, 890, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 818, 1106
HpyF3I CTNAG 7 cut(s) 276, 327, 614, 645, 687, 765, 798
HpySE526I ACGT 2 cut(s) 424, 481
Hsp92II CATG 1 cut(s) 1121
Ksp22I TGATCA 1 cut(s) 1036
Kzo9I GATC 3 cut(s) 28, 388, 1036
LmnI GCTCC 2 cut(s) 529, 877
Lsp1109I GCAGC 2 cut(s) 297, 1096
LweI GCATC 5 cut(s) 442, 571, 594, 769, 791
MaeI CTAG 2 cut(s) 705, 1126
MaeII ACGT 2 cut(s) 424, 481
MaeIII GTNAC 2 cut(s) 168, 741
MalI GATC 3 cut(s) 30, 390, 1038
MboI GATC 3 cut(s) 28, 388, 1036
MboII GAAGA 9 cut(s) 479, 508, 587, 648, 659, 783, 946, 990, 999
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 1 cut(s) 695
MluCI AATT 6 cut(s) 70, 231, 281, 296, 772, 947
MlyI GAGTC 2 cut(s) 417, 715
Mph1103I ATGCAT 1 cut(s) 784
MseI TTAA 2 cut(s) 132, 303
MspA1I CMGCKG 1 cut(s) 1077
MspI CCGG 1 cut(s) 527
MspR9I CCNGG 2 cut(s) 718, 787
MvaI CCWGG 2 cut(s) 718, 787
MwoI GCNNNNNNNGC 3 cut(s) 272, 818, 1106
NdeII GATC 3 cut(s) 28, 388, 1036
NlaIII CATG 1 cut(s) 1121
NlaIV GGNNCC 2 cut(s) 30, 60
NmuCI GTSAC 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 784
NspV TTCGAA 1 cut(s) 957
PaeR7I CTCGAG 1 cut(s) 405
PfeI GAWTC 4 cut(s) 365, 499, 959, 965
PflFI GACNNNGTC 2 cut(s) 483, 1084
PflMI CCANNNNNTGG 1 cut(s) 428
PfoI TCCNGGA 1 cut(s) 716
PkrI GCNGC 3 cut(s) 312, 814, 1111
PleI GAGTC 2 cut(s) 416, 715
PpsI GAGTC 2 cut(s) 416, 715
Psp6I CCWGG 2 cut(s) 716, 785
PspEI GGTNACC 1 cut(s) 741
PspGI CCWGG 2 cut(s) 716, 785
PspN4I GGNNCC 2 cut(s) 30, 60
PspPI GGNCC 4 cut(s) 44, 58, 901, 1086
PstI CTGCAG 1 cut(s) 1114
PsuI RGATCY 1 cut(s) 28
PsyI GACNNNGTC 2 cut(s) 483, 1084
PvuII CAGCTG 1 cut(s) 1077
RsaI GTAC 2 cut(s) 21, 379
RsaNI GTAC 2 cut(s) 20, 378
SaqAI TTAA 2 cut(s) 132, 303
SatI GCNGC 3 cut(s) 311, 813, 1110
Sau3AI GATC 3 cut(s) 28, 388, 1036
Sau96I GGNCC 4 cut(s) 44, 58, 901, 1086
ScaI AGTACT 2 cut(s) 21, 379
SchI GAGTC 2 cut(s) 417, 715
ScrFI CCNGG 2 cut(s) 718, 787
SduI GDGCHC 1 cut(s) 695
SfaNI GCATC 5 cut(s) 442, 571, 594, 769, 791
SfcI CTRYAG 2 cut(s) 1072, 1110
Sfr274I CTCGAG 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 957
SinI GGWCC 2 cut(s) 58, 1086
SlaI CTCGAG 1 cut(s) 405
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
Sse9I AATT 6 cut(s) 70, 231, 281, 296, 772, 947
SsiI CCGC 3 cut(s) 355, 749, 812
SspMI CTAG 2 cut(s) 705, 1126
StyD4I CCNGG 2 cut(s) 716, 785
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 3 cut(s) 535, 830, 1057
TaiI ACGT 2 cut(s) 427, 484
TaqI TCGA 6 cut(s) 115, 406, 478, 670, 823, 957
TasI AATT 6 cut(s) 70, 231, 281, 296, 772, 947
TatI WGTACW 2 cut(s) 19, 377
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 4 cut(s) 365, 499, 959, 965
Tru1I TTAA 2 cut(s) 132, 303
Tru9I TTAA 2 cut(s) 132, 303
TscAI CASTG 1 cut(s) 903
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 2 cut(s) 310, 1109
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 4 cut(s) 326, 555, 630, 908
TspGWI ACGGA 2 cut(s) 240, 429
TspRI CASTG 1 cut(s) 903
Tth111I GACNNNGTC 2 cut(s) 483, 1084
Van91I CCANNNNNTGG 1 cut(s) 428
VpaK11BI GGWCC 2 cut(s) 58, 1086
XapI RAATTY 3 cut(s) 231, 772, 947
XcmI CCANNNNNNNNNTGG 2 cut(s) 735, 1011
XhoI CTCGAG 1 cut(s) 405
XspI CTAG 2 cut(s) 705, 1126
ZrmI AGTACT 2 cut(s) 21, 379
Zsp2I ATGCAT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.