Rw1G010880
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0017399
Physical Location & Seq
Reverse (-)
24614887 .. 24616941
2055 bp
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UTR
Exon/CDS
Intron
Rw1G010880.1

Sequence Viewer

Length: 261 bp
ATGAGAGGTGAAGGAGCTATTGCAGTATTGGACGCTGCCGCTGGAGAGGATTCAAATTTCTTGGAGGATGAAAGCACAAAGATATTTGCACGTAAAGTGGCCGATCATTATAGTGCAAGGAAAAATCAAACTCTGGAAGAACGAGAAGCTAGTCCGATCATCCATTTAAAAAAGCTTAACAACTGGATTAAGAGTGTACTAATTCAGCTCTATGCTCGCCGAGGAGATGCTGTTCTTGATCTTGCATGCGGAAAGGTTTAA

Protein Analysis

86

Amino Acids

9.54

Weight (kDa)

6.72

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mRNA_G-N7_MeTrfase PF03291 22 - 85 5.3e-20 mRNA (guanine-N(7))-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 39, 249
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 1 cut(s) 55
AfaI GTAC 1 cut(s) 198
AgsI TTSAA 1 cut(s) 54
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
AluBI AGCT 4 cut(s) 17, 149, 175, 208
AluI AGCT 4 cut(s) 17, 149, 175, 208
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 20
BbvI GCAGC 1 cut(s) 22
BfaI CTAG 1 cut(s) 150
BisI GCNGC 2 cut(s) 36, 39
BlsI GCNGC 2 cut(s) 37, 40
BmsI GCATC 1 cut(s) 217
BpmI CTGGAG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bse1I ACTGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 2 cut(s) 73, 159
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 22
BshFI GGCC 1 cut(s) 101
BsnI GGCC 1 cut(s) 101
Bsp143I GATC 3 cut(s) 103, 156, 238
BspACI CCGC 2 cut(s) 39, 249
BspANI GGCC 1 cut(s) 101
BsrI ACTGG 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 3 cut(s) 103, 156, 238
BstBAI YACGTR 1 cut(s) 92
BstC8I GCNNGC 2 cut(s) 217, 247
BstF5I GGATG 2 cut(s) 73, 159
BstKTI GATC 3 cut(s) 106, 159, 241
BstMBI GATC 3 cut(s) 103, 156, 238
BstNSI RCATGY 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 22
BsuRI GGCC 1 cut(s) 101
BtsCI GGATG 2 cut(s) 73, 159
Cac8I GCNNGC 2 cut(s) 217, 247
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 197
CviAII CATG 1 cut(s) 246
CviJI RGCY 5 cut(s) 17, 101, 149, 175, 208
CviKI_1 RGCY 5 cut(s) 17, 101, 149, 175, 208
CviQI GTAC 1 cut(s) 197
DpnI GATC 3 cut(s) 105, 158, 240
DpnII GATC 3 cut(s) 103, 156, 238
DraI TTTAAA 1 cut(s) 168
EaeI YGGCCR 1 cut(s) 99
FaeI CATG 1 cut(s) 249
FaiI YATR 3 cut(s) 111, 213, 247
FatI CATG 1 cut(s) 245
Fnu4HI GCNGC 2 cut(s) 36, 39
FokI GGATG 2 cut(s) 80, 146
Fsp4HI GCNGC 2 cut(s) 36, 39
FspBI CTAG 1 cut(s) 150
GluI GCNGC 2 cut(s) 36, 39
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 101
HgaI GACGC 1 cut(s) 41
Hin1II CATG 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 1 cut(s) 50
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 1 cut(s) 197
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 134, 236
Hpy8I GTNNAC 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 5
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 4 cut(s) 23, 89, 116, 245
HpySE526I ACGT 1 cut(s) 91
Hsp92II CATG 1 cut(s) 249
Kzo9I GATC 3 cut(s) 103, 156, 238
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 3 cut(s) 27, 119, 169
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 1 cut(s) 217
MaeI CTAG 1 cut(s) 150
MaeII ACGT 1 cut(s) 91
MalI GATC 3 cut(s) 105, 158, 240
MboI GATC 3 cut(s) 103, 156, 238
MboII GAAGA 1 cut(s) 149
MluCI AATT 2 cut(s) 55, 201
MnlI CCTC 3 cut(s) 40, 58, 215
MseI TTAA 4 cut(s) 167, 177, 189, 259
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 41
NdeII GATC 3 cut(s) 103, 156, 238
NlaIII CATG 1 cut(s) 249
NmeAIII GCCGAG 1 cut(s) 245
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 50
PkrI GCNGC 2 cut(s) 37, 40
Ppu21I YACGTR 1 cut(s) 92
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 111
SaqAI TTAA 4 cut(s) 167, 177, 189, 259
SatI GCNGC 2 cut(s) 36, 39
Sau3AI GATC 3 cut(s) 103, 156, 238
SetI ASST 7 cut(s) 10, 19, 94, 151, 177, 210, 258
SfaNI GCATC 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 111
SphI GCATGC 1 cut(s) 249
Sse9I AATT 2 cut(s) 55, 201
SsiI CCGC 2 cut(s) 39, 249
SspMI CTAG 1 cut(s) 150
TaiI ACGT 1 cut(s) 94
TasI AATT 2 cut(s) 55, 201
TatI WGTACW 1 cut(s) 196
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 50
Tru1I TTAA 4 cut(s) 167, 177, 189, 259
Tru9I TTAA 4 cut(s) 167, 177, 189, 259
TseI GCWGC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 84
XapI RAATTY 1 cut(s) 55
XceI RCATGY 1 cut(s) 249
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.