Rw2G019180
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017399
Physical Location & Seq
Forward (+)
25180183 .. 25182254
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G019180.1

Sequence Viewer

Length: 333 bp
ATGAGAGGTGAAGGAGCTATTGCAGTATTGGACGCTGCCGCTGGAGAGGATTCAAATTTCTTGGAGGATGAAAGCACAAAGATATTTGCACGTAAAGTGGCCGATCATTATAGTGCAAGGACAAATCAAACTCTGGAAGAACGAGAAGCTAGTCCGATCATCCATTTAAAAAAGCTTAACAACTGGATTAAGAGTGTACTAATTCAGCTCTATGCTCGCCGAGGAGATGCTGTTCTTGATCTTGCATGCGGAAAGGGAGGTGATCTCATCAAATGGGACAAGGCCAAAATTGGGTACTATGTTGGCATTGATATTGCTGAAGGCTCTGTTTAA

Protein Analysis

110

Amino Acids

12.06

Weight (kDa)

5.66

Isoelectric Point (pI)

23.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mRNA_G-N7_MeTrfase PF03291 22 - 110 1.4e-35 mRNA (guanine-N(7))-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 39, 249
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 1 cut(s) 55
AfaI GTAC 2 cut(s) 198, 296
AfiI CCNNNNNNNGG 1 cut(s) 291
AgsI TTSAA 1 cut(s) 54
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
AluBI AGCT 4 cut(s) 17, 149, 175, 208
AluI AGCT 4 cut(s) 17, 149, 175, 208
AoxI GGCC 2 cut(s) 99, 282
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 1 cut(s) 55
AsuHPI GGTGA 2 cut(s) 20, 272
BbvI GCAGC 1 cut(s) 22
BfaI CTAG 1 cut(s) 150
BisI GCNGC 2 cut(s) 36, 39
BlsI GCNGC 2 cut(s) 37, 40
BmsI GCATC 1 cut(s) 217
BplI GAGNNNNNCTC 2 cut(s) 249, 281
BpmI CTGGAG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse1I ACTGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 2 cut(s) 73, 159
BseLI CCNNNNNNNGG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 22
BshFI GGCC 2 cut(s) 101, 284
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 2 cut(s) 101, 284
Bsp143I GATC 4 cut(s) 103, 156, 238, 262
BspACI CCGC 2 cut(s) 39, 249
BspANI GGCC 2 cut(s) 101, 284
BsrI ACTGG 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 4 cut(s) 103, 156, 238, 262
BstBAI YACGTR 1 cut(s) 92
BstC8I GCNNGC 2 cut(s) 217, 247
BstF5I GGATG 2 cut(s) 73, 159
BstKTI GATC 4 cut(s) 106, 159, 241, 265
BstMBI GATC 4 cut(s) 103, 156, 238, 262
BstNSI RCATGY 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 22
BsuRI GGCC 2 cut(s) 101, 284
BtsCI GGATG 2 cut(s) 73, 159
Cac8I GCNNGC 2 cut(s) 217, 247
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 197, 295
CviAII CATG 1 cut(s) 246
CviJI RGCY 7 cut(s) 17, 101, 149, 175, 208, 284, 324
CviKI_1 RGCY 7 cut(s) 17, 101, 149, 175, 208, 284, 324
CviQI GTAC 2 cut(s) 197, 295
DpnI GATC 4 cut(s) 105, 158, 240, 264
DpnII GATC 4 cut(s) 103, 156, 238, 262
DraI TTTAAA 1 cut(s) 168
EaeI YGGCCR 1 cut(s) 99
FaeI CATG 1 cut(s) 249
FaiI YATR 4 cut(s) 111, 213, 247, 300
FaqI GGGAC 1 cut(s) 290
FatI CATG 1 cut(s) 245
Fnu4HI GCNGC 2 cut(s) 36, 39
FokI GGATG 2 cut(s) 80, 146
Fsp4HI GCNGC 2 cut(s) 36, 39
FspBI CTAG 1 cut(s) 150
GluI GCNGC 2 cut(s) 36, 39
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 2 cut(s) 101, 284
HgaI GACGC 1 cut(s) 41
Hin1II CATG 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 1 cut(s) 50
HphI GGTGA 2 cut(s) 20, 272
Hpy166II GTNNAC 1 cut(s) 197
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 134, 236
Hpy8I GTNNAC 1 cut(s) 197
HpyAV CCTTC 2 cut(s) 5, 314
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 4 cut(s) 23, 89, 116, 245
HpySE526I ACGT 1 cut(s) 91
Hsp92II CATG 1 cut(s) 249
Kzo9I GATC 4 cut(s) 103, 156, 238, 262
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 3 cut(s) 27, 119, 169
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 1 cut(s) 217
MaeI CTAG 1 cut(s) 150
MaeII ACGT 1 cut(s) 91
MalI GATC 4 cut(s) 105, 158, 240, 264
MboI GATC 4 cut(s) 103, 156, 238, 262
MboII GAAGA 1 cut(s) 149
MluCI AATT 3 cut(s) 55, 201, 288
MnlI CCTC 4 cut(s) 40, 58, 215, 251
MseI TTAA 4 cut(s) 167, 177, 189, 331
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 41
NdeII GATC 4 cut(s) 103, 156, 238, 262
NlaIII CATG 1 cut(s) 249
NmeAIII GCCGAG 1 cut(s) 245
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 50
PkrI GCNGC 2 cut(s) 37, 40
Ppu21I YACGTR 1 cut(s) 92
RsaI GTAC 2 cut(s) 198, 296
RsaNI GTAC 2 cut(s) 197, 295
RseI CAYNNNNRTG 1 cut(s) 111
SaqAI TTAA 4 cut(s) 167, 177, 189, 331
SatI GCNGC 2 cut(s) 36, 39
Sau3AI GATC 4 cut(s) 103, 156, 238, 262
SetI ASST 7 cut(s) 10, 19, 94, 151, 177, 210, 262
SfaNI GCATC 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 111
SphI GCATGC 1 cut(s) 249
Sse9I AATT 3 cut(s) 55, 201, 288
SsiI CCGC 2 cut(s) 39, 249
SspMI CTAG 1 cut(s) 150
TaiI ACGT 1 cut(s) 94
TasI AATT 3 cut(s) 55, 201, 288
TatI WGTACW 1 cut(s) 196
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 50
Tru1I TTAA 4 cut(s) 167, 177, 189, 331
Tru9I TTAA 4 cut(s) 167, 177, 189, 331
TseI GCWGC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 84
XapI RAATTY 1 cut(s) 55
XceI RCATGY 1 cut(s) 249
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.