Rw2G047690

leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015232
Physical Location & Seq
Forward (+)
74996440 .. 74998570
2131 bp
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UTR
Exon/CDS
Intron
Rw2G047690.1

Sequence Viewer

Length: 786 bp
ATGACAGATAATCTTTGGCGTTTTTGGCCTGCAAGATGTTCTTATTGCTTGAATATCAGAAGACGCCAAGGAGTGGTTTATAAAGCTGATCTGGTTGTCATTGGAATCTTTGTTGGTGTAGTCCATGGAATTTTGATAGCTGCACTCATATTTTTCAGAATAAAGGTGTGGAATAATCACAACAAAGATGGATTTCCTACTGCATCAGGTGTACTTAGATACTCTTACAAGTATGCATTAAATGCTGTATCATTTAGGGATGTACAGAAAGCTACACAGAACTTCGCAACCATTTGGGGACAAGGGCCTTTTGGTCCAGTCAATAAAGCAACAATGCCTGCTGGAAAGGTAGCAGCTGTGAAGGTGCTTGCTTCAAATTCCAAGCAGGGAGAGAAAGAGTTCCAGACAGAGGAAAAGACTACATCAGCTACATTTCGAATAGTGTATGTTAATCTAGGGAGGTTCTGCAGGTATCACTACAATGTTTTGGATGTGAGAGTGGACTGGCACATTGACAAAGGAAAGGAAGATGGTTTGTATATTAGCTGTGTCGCCTCATCTGCGAATATGTGGGCTATAGTCATGGATGCAGGGACAGGAGTGGATAATGGAACAGTGGGACAAGAACTACCATATCACTTCACTTGCTGGCGCAGGCAATGGCAGTGCCTTGGTGGTAATGTCCAAAGGTTGTTGAGATTGATTTCCTATATCCAAGCGAAGGCATCCATGGAAGATGGGAGAATGGATATCGCATTTACCTCAACTGCTGCAACAGAGGATTAA

Protein Analysis

261

Amino Acids

29.41

Weight (kDa)

9.37

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7477 PF24289 156 - 203 1.1e-18 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
Acc36I ACCTGC 1 cut(s) 459
AccB7I CCANNNNNTGG 1 cut(s) 73
AcsI RAATTY 2 cut(s) 129, 376
AcyI GRCGYC 1 cut(s) 64
AfaI GTAC 2 cut(s) 213, 264
AfiI CCNNNNNNNGG 3 cut(s) 73, 409, 721
AgsI TTSAA 2 cut(s) 52, 375
AluBI AGCT 6 cut(s) 86, 140, 272, 356, 428, 546
AluI AGCT 6 cut(s) 86, 140, 272, 356, 428, 546
AoxI GGCC 2 cut(s) 26, 305
ApeKI GCWGC 3 cut(s) 140, 353, 770
ApoI RAATTY 2 cut(s) 129, 376
Asp700I GAANNNNTTC 1 cut(s) 398
AspLEI GCGC 1 cut(s) 654
AspS9I GGNCC 2 cut(s) 305, 314
AsuII TTCGAA 1 cut(s) 436
AvaII GGWCC 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 67
BbvI GCAGC 3 cut(s) 127, 365, 757
BccI CCATC 3 cut(s) 182, 524, 731
BfaI CTAG 1 cut(s) 455
BfmI CTRYAG 2 cut(s) 466, 576
BfuAI ACCTGC 1 cut(s) 459
BisI GCNGC 3 cut(s) 141, 354, 771
BlsI GCNGC 3 cut(s) 142, 355, 772
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 305, 314
BmsI GCATC 3 cut(s) 212, 577, 734
BpiI GAAGAC 1 cut(s) 67
Bpu14I TTCGAA 1 cut(s) 436
BsaHI GRCGYC 1 cut(s) 64
BsaJI CCNNGG 4 cut(s) 67, 124, 670, 729
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 409, 721
Bse1I ACTGG 2 cut(s) 317, 509
Bse3DI GCAATG 1 cut(s) 665
BseDI CCNNGG 4 cut(s) 67, 124, 670, 729
BseGI GGATG 4 cut(s) 265, 496, 592, 725
BseLI CCNNNNNNNGG 3 cut(s) 73, 409, 721
BseMI GCAATG 1 cut(s) 665
BseNI ACTGG 2 cut(s) 317, 509
BseXI GCAGC 3 cut(s) 127, 365, 757
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 2 cut(s) 28, 307
BslFI GGGAC 3 cut(s) 312, 607, 633
BslI CCNNNNNNNGG 3 cut(s) 73, 409, 721
BsmFI GGGAC 3 cut(s) 312, 607, 633
BsnI GGCC 2 cut(s) 28, 307
Bsp119I TTCGAA 1 cut(s) 436
Bsp1407I TGTACA 1 cut(s) 262
Bsp143I GATC 1 cut(s) 88
Bsp19I CCATGG 2 cut(s) 124, 729
BspANI GGCC 2 cut(s) 28, 307
BspMAI CTGCAG 1 cut(s) 470
BspMI ACCTGC 1 cut(s) 459
BspT104I TTCGAA 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 665
BsrGI TGTACA 1 cut(s) 262
BsrI ACTGG 2 cut(s) 317, 509
BssECI CCNNGG 4 cut(s) 67, 124, 670, 729
BssMI GATC 1 cut(s) 88
BssNI GRCGYC 1 cut(s) 64
BssT1I CCWWGG 4 cut(s) 67, 124, 670, 729
Bst4CI ACNGT 1 cut(s) 616
BstACI GRCGYC 1 cut(s) 64
BstAPI GCANNNNNTGC 1 cut(s) 242
BstAUI TGTACA 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 436
BstC8I GCNNGC 5 cut(s) 30, 339, 369, 650, 656
BstDEI CTNAG 1 cut(s) 215
BstDSI CCRYGG 2 cut(s) 124, 729
BstF5I GGATG 4 cut(s) 265, 496, 592, 725
BstHHI GCGC 1 cut(s) 654
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 3 cut(s) 25, 242, 560
BstSFI CTRYAG 2 cut(s) 466, 576
BstV1I GCAGC 3 cut(s) 127, 365, 757
BstV2I GAAGAC 1 cut(s) 67
BsuRI GGCC 2 cut(s) 28, 307
BtgI CCRYGG 2 cut(s) 124, 729
BtsCI GGATG 4 cut(s) 265, 496, 592, 725
BtsI GCAGTG 1 cut(s) 671
BtsIMutI CAGTG 2 cut(s) 621, 671
BveI ACCTGC 1 cut(s) 459
Cac8I GCNNGC 5 cut(s) 30, 339, 369, 650, 656
CfoI GCGC 1 cut(s) 654
Cfr13I GGNCC 2 cut(s) 305, 314
CseI GACGC 1 cut(s) 72
Csp6I GTAC 2 cut(s) 212, 263
CviAII CATG 3 cut(s) 125, 583, 730
CviJI RGCY 9 cut(s) 28, 86, 140, 272, 307, 356, 428, 546, 575
CviKI_1 RGCY 9 cut(s) 28, 86, 140, 272, 307, 356, 428, 546, 575
CviQI GTAC 2 cut(s) 212, 263
DdeI CTNAG 1 cut(s) 215
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
Eco130I CCWWGG 4 cut(s) 67, 124, 670, 729
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 305
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 4 cut(s) 67, 124, 670, 729
EcoT22I ATGCAT 1 cut(s) 238
ErhI CCWWGG 4 cut(s) 67, 124, 670, 729
FaeI CATG 3 cut(s) 128, 586, 733
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FaqI GGGAC 3 cut(s) 312, 607, 633
FatI CATG 3 cut(s) 124, 582, 729
Fnu4HI GCNGC 3 cut(s) 141, 354, 771
FokI GGATG 4 cut(s) 272, 503, 599, 712
Fsp4HI GCNGC 3 cut(s) 141, 354, 771
FspBI CTAG 1 cut(s) 455
GlaI GCGC 1 cut(s) 653
GluI GCNGC 3 cut(s) 141, 354, 771
HaeIII GGCC 2 cut(s) 28, 307
HgaI GACGC 1 cut(s) 72
HhaI GCGC 1 cut(s) 654
Hin1I GRCGYC 1 cut(s) 64
Hin1II CATG 3 cut(s) 128, 586, 733
Hin6I GCGC 1 cut(s) 652
HinP1I GCGC 1 cut(s) 652
HinfI GANTC 1 cut(s) 105
Hpy166II GTNNAC 2 cut(s) 212, 502
Hpy188I TCNGA 2 cut(s) 59, 158
Hpy188III TCNNGA 1 cut(s) 403
Hpy8I GTNNAC 2 cut(s) 212, 502
HpyAV CCTTC 2 cut(s) 355, 715
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4V TGCA 7 cut(s) 32, 143, 203, 236, 468, 590, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 242, 560
HpyF3I CTNAG 1 cut(s) 215
Hsp92I GRCGYC 1 cut(s) 64
Hsp92II CATG 3 cut(s) 128, 586, 733
HspAI GCGC 1 cut(s) 652
Kzo9I GATC 1 cut(s) 88
Lsp1109I GCAGC 3 cut(s) 127, 365, 757
LweI GCATC 3 cut(s) 212, 577, 734
MaeI CTAG 1 cut(s) 455
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 3 cut(s) 72, 539, 746
MluCI AATT 2 cut(s) 129, 376
MnlI CCTC 5 cut(s) 403, 453, 565, 772, 772
Mph1103I ATGCAT 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 398
MseI TTAA 3 cut(s) 239, 450, 784
MslI CAYNNNNRTG 1 cut(s) 480
MspA1I CMGCKG 1 cut(s) 356
MwoI GCNNNNNNNGC 3 cut(s) 25, 242, 560
NcoI CCATGG 2 cut(s) 124, 729
NdeII GATC 1 cut(s) 88
NlaIII CATG 3 cut(s) 128, 586, 733
NsiI ATGCAT 1 cut(s) 238
NspV TTCGAA 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 398
PfeI GAWTC 1 cut(s) 105
PflMI CCANNNNNTGG 1 cut(s) 73
PkrI GCNGC 3 cut(s) 142, 355, 772
PsiI TTATAA 1 cut(s) 81
PspPI GGNCC 2 cut(s) 305, 314
PstI CTGCAG 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 2 cut(s) 213, 264
RsaNI GTAC 2 cut(s) 212, 263
RseI CAYNNNNRTG 1 cut(s) 480
SaqAI TTAA 3 cut(s) 239, 450, 784
SatI GCNGC 3 cut(s) 141, 354, 771
Sau3AI GATC 1 cut(s) 88
Sau96I GGNCC 2 cut(s) 305, 314
SfaNI GCATC 3 cut(s) 212, 577, 734
SfcI CTRYAG 2 cut(s) 466, 576
SfuI TTCGAA 1 cut(s) 436
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 480
Sse9I AATT 2 cut(s) 129, 376
SspMI CTAG 1 cut(s) 455
StyI CCWWGG 4 cut(s) 67, 124, 670, 729
TaaI ACNGT 1 cut(s) 616
TaqI TCGA 1 cut(s) 436
TasI AATT 2 cut(s) 129, 376
TatI WGTACW 2 cut(s) 211, 262
TfiI GAWTC 1 cut(s) 105
Tru1I TTAA 3 cut(s) 239, 450, 784
Tru9I TTAA 3 cut(s) 239, 450, 784
TscAI CASTG 2 cut(s) 621, 671
TseI GCWGC 3 cut(s) 140, 353, 770
TspRI CASTG 2 cut(s) 621, 671
Van91I CCANNNNNTGG 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 2 cut(s) 129, 376
XmnI GAANNNNTTC 1 cut(s) 398
XspI CTAG 1 cut(s) 455
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.