Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009374

Physical Location & Seq

Genomic Coordinates Forward (+)

81529237 .. 81531280

Gene Structure 2044 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G053840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1179 bp

ATGTACCTCTCCGAAAAGCCCCACCCAATTGATTTCTACAAGTCCGTCGACGCCTCCTCCTCCCCCGACCGCGACATGATCATGGAGGTCGTCTCCGCCGCCGCCGCCGCCAACGCCGCCGCTCCAGGTGGCAACACCAGCAGCGGCGGCGAGGACCACGAGGTCCGCGCCCCGAAGAAGCGGGCCGAGCATTGGATCCAGGAAGAGACGCGGTGCCTCATCAGCTTCCGCCGCGAGGTCGATGGCCTCTTCAACACCTCCAAGTCCAACAAGCACCTCTGGGAGCAAATCTCGGGCAAGATGAGGGATAAAGGGTTCGATCGGAGCCCCACCATGTGTACGGACAAGTGGAGGAACTTGCTTAAGGAGTTCAAGAAGGCCAGGCAGCAGGTCAAGGTGGGCAGCAGGTCTGCCAAGATGTTGTATTACGAGGACTTGCAGGAGTTGATTAGGGATAGGACTAGCCATAATAATAGTACCAATGCTGCTAATAATGCCGCTGCTGCTGCTTATAAGAGGCCCAATTCTCCTCCGAAGCTCGAATCTTTCATTCGTTTCTCTGATAAGAGTCTCGAGGACGCAGGTATTCCGTTTGGACCCATGGAAGCTGACCGAACAGCAGTGGACTTGGAAAGGCAGTTGGATCATGATGGTGACCCTCTGGCTATTACTGCGGCTGAGGCAGTGGCAGCCAACGAGGTTCCTCCGTGGAATTGGAGGGAGGGCCCTGAGAATGGAGGGGAGACACACTCATCTTTTTGTGGAAGGATTATAACTGTCAAGTTGGGGGAATACAACACCAGAAGGATTGGTATTGATGGAACAGCTGATGCGATTAAGGAGGCAATCAAGTCTGCATTCAGAATAAGAACTAAGCGAGCATTTTGGTTGGAGGACGAGTATCAGGTTGTTCGGAGTCTTGATAGGGATATGCCTTTGGGCAATTATACTCTCCACCTTGATGAAGGAATAACCATTAAACTTTGCCTCTGCGATGAATCTGACCGTATAGTTCGCACAGAAGAAATAACATTCTACACTGAGGATGACTTTCGTGATTTCCTTTCACGTCGCGGCTTGATTGGTTTAAGAGAATTAGGTGGCTACAGACGCATTGATACTTTAGATGATCTTCAATCTGGTGCAATGTATCAGGGGGTGAGACTTCTGGGGGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-bind_4

Protein Analysis

392

Amino Acids

44.26

Weight (kDa)

5.77

Isoelectric Point (pI)

43.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-bind_4	PF13837	64 - 149	3.9e-21	Myb/SANT-like DNA-binding domain
Ubiquitin_GT-1	PF26214	256 - 317	9.6e-10	GT-1-like, ubiquitin-like domain
Ubiquitin_GT-1	PF26214	322 - 386	1.6e-29	GT-1-like, ubiquitin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009374)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13450 AT1G13450 AT1G13450
fragaria_vesca	FvH4_6g52740
malus_domestica	MD06G1052100.v1.1 MD17G1017400.v1.1
prunus_persica	Prupe.3G300500_v2.0.a1
pyrus_communis	pycom06g06930 pycom16g09330 pycom17g01400
rosa_chinensis	RchiOBHm_Chr2g0174361
rosa_laevigata	RLG00000022260
rosa_multiflora	Rmu_co8108256.1_g000001 Rmu_co8252927.1_g000001 Rmu_sc0003649.1_g000031
rosa_roxburghii	Rroxscaffold_2G00078270
rosa_rugosa	Rorug02G0576400
rosa_samantha	Rh2AG657400 Rh2BG668400 Rh2CG631500 Rh2DG682000
rosa_wichuraiana	Rw2G053840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	513, 773
AasI	GACNNNNNNGTC	1 cut(s)	161
Acc36I	ACCTGC	3 cut(s)	379, 396, 572
AccB1I	GGYRCC	1 cut(s)	213
AccBSI	CCGCTC	1 cut(s)	122
AccI	GTMKAC	1 cut(s)	48
AccII	CGCG	5 cut(s)	72, 168, 211, 234, 1074
AclWI	GGATC	3 cut(s)	190, 203, 651
AcyI	GRCGYC	1 cut(s)	51
AdeI	CACNNNGTG	1 cut(s)	336
AfaI	GTAC	3 cut(s)	5, 340, 478
AfiI	CCNNNNNNNGG	3 cut(s)	192, 235, 734
AflII	CTTAAG	1 cut(s)	362
AgsI	TTSAA	3 cut(s)	253, 373, 1136
AjiI	CACGTC	1 cut(s)	1070
AjnI	CCWGG	3 cut(s)	124, 198, 380
AloI	GAACNNNNNNTCC	2 cut(s)	299, 331
AluBI	AGCT	4 cut(s)	225, 538, 608, 827
AluI	AGCT	4 cut(s)	225, 538, 608, 827
Alw26I	GTCTC	5 cut(s)	97, 200, 575, 737, 1156
AlwI	GGATC	3 cut(s)	190, 203, 651
Ama87I	CYCGRG	2 cut(s)	292, 572
AoxI	GGCC	5 cut(s)	183, 244, 378, 518, 724
ApaI	GGGCCC	1 cut(s)	728
ApeKI	GCWGC	8 cut(s)	141, 385, 402, 485, 500, 503, 506, 689
AspLEI	GCGC	1 cut(s)	170
AspS9I	GGNCC	7 cut(s)	154, 163, 183, 519, 596, 724, 725
AsuHPI	GGTGA	2 cut(s)	665, 1171
AvaI	CYCGRG	2 cut(s)	292, 572
AvaII	GGWCC	3 cut(s)	154, 163, 596
BaeGI	GKGCMC	1 cut(s)	728
BamHI	GGATCC	1 cut(s)	195
BanI	GGYRCC	1 cut(s)	213
BanII	GRGCYC	2 cut(s)	329, 728
BauI	CACGAG	1 cut(s)	158
BbvCI	CCTCAGC	1 cut(s)	678
BbvI	GCAGC	8 cut(s)	153, 397, 414, 472, 487, 490, 493, 701
BccI	CCATC	3 cut(s)	236, 644, 812
BciT130I	CCWGG	3 cut(s)	126, 200, 382
BclI	TGATCA	1 cut(s)	78
BcoDI	GTCTC	5 cut(s)	97, 200, 575, 737, 1156
BfaI	CTAG	2 cut(s)	462, 1177
BfmI	CTRYAG	1 cut(s)	1105
BfrI	CTTAAG	1 cut(s)	362
BfuAI	ACCTGC	3 cut(s)	379, 396, 572
Bme1390I	CCNGG	3 cut(s)	126, 200, 382
Bme18I	GGWCC	3 cut(s)	154, 163, 596
BmeT110I	CYCGRG	2 cut(s)	292, 572
BmgBI	CACGTC	1 cut(s)	1070
BmgT120I	GGNCC	7 cut(s)	154, 163, 183, 519, 596, 724, 725
BmiI	GGNNCC	6 cut(s)	197, 215, 326, 598, 702, 726
BmrFI	CCNGG	3 cut(s)	126, 200, 382
BmsI	GCATC	1 cut(s)	820
BplI	GAGNNNNNCTC	6 cut(s)	77, 109, 275, 307, 734, 766
BpmI	CTGGAG	1 cut(s)	108
Bpu10I	CCTNAGC	1 cut(s)	678
BsaHI	GRCGYC	1 cut(s)	51
BsaJI	CCNNGG	2 cut(s)	600, 707
BsaXI	ACNNNNNCTCC	4 cut(s)	41, 71, 884, 914
Bsc4I	CCNNNNNNNGG	3 cut(s)	192, 235, 734
Bse3DI	GCAATG	1 cut(s)	1152
BseBI	CCWGG	3 cut(s)	126, 200, 382
BseDI	CCNNGG	2 cut(s)	600, 707
BseGI	GGATG	1 cut(s)	1051
BseLI	CCNNNNNNNGG	3 cut(s)	192, 235, 734
BseMI	GCAATG	1 cut(s)	1152
BseMII	CTCAG	3 cut(s)	669, 720, 1032
BseRI	GAGGAG	3 cut(s)	46, 49, 519
BseSI	GKGCMC	1 cut(s)	728
BseXI	GCAGC	8 cut(s)	153, 397, 414, 472, 487, 490, 493, 701
Bsh1236I	CGCG	5 cut(s)	72, 168, 211, 234, 1074
Bsh1285I	CGRYCG	2 cut(s)	70, 322
BshFI	GGCC	5 cut(s)	185, 246, 380, 520, 726
BshNI	GGYRCC	1 cut(s)	213
BsiEI	CGRYCG	2 cut(s)	70, 322
BsiHKCI	CYCGRG	2 cut(s)	292, 572
BslI	CCNNNNNNNGG	3 cut(s)	192, 235, 734
BsmAI	GTCTC	5 cut(s)	97, 200, 575, 737, 1156
BsmBI	CGTCTC	2 cut(s)	97, 200
BsmI	GAATGC	1 cut(s)	857
BsnI	GGCC	5 cut(s)	185, 246, 380, 520, 726
BsoBI	CYCGRG	2 cut(s)	292, 572
Bsp120I	GGGCCC	1 cut(s)	724
Bsp1286I	GDGCHC	2 cut(s)	329, 728
Bsp143I	GATC	5 cut(s)	78, 195, 319, 643, 1129
Bsp19I	CCATGG	1 cut(s)	600
BspANI	GGCC	5 cut(s)	185, 246, 380, 520, 726
BspCNI	CTCAG	3 cut(s)	670, 721, 1033
BspFNI	CGCG	5 cut(s)	72, 168, 211, 234, 1074
BspHI	TCATGA	1 cut(s)	646
BspLI	GGNNCC	6 cut(s)	197, 215, 326, 598, 702, 726
BspMI	ACCTGC	3 cut(s)	379, 396, 572
BspPI	GGATC	3 cut(s)	190, 203, 651
BspT107I	GGYRCC	1 cut(s)	213
BspTI	CTTAAG	1 cut(s)	362
BsrBI	CCGCTC	1 cut(s)	122
BsrDI	GCAATG	1 cut(s)	1152
BssECI	CCNNGG	2 cut(s)	600, 707
BssMI	GATC	5 cut(s)	78, 195, 319, 643, 1129
BssNI	GRCGYC	1 cut(s)	51
BssSI	CACGAG	1 cut(s)	158
BssT1I	CCWWGG	1 cut(s)	600
Bst2BI	CACGAG	1 cut(s)	158
Bst2UI	CCWGG	3 cut(s)	126, 200, 382
Bst4CI	ACNGT	2 cut(s)	778, 1007
Bst6I	CTCTTC	2 cut(s)	198, 254
BstACI	GRCGYC	1 cut(s)	51
BstAFI	CTTAAG	1 cut(s)	362
BstC8I	GCNNGC	2 cut(s)	183, 879
BstDEI	CTNAG	4 cut(s)	678, 729, 873, 1041
BstDSI	CCRYGG	2 cut(s)	600, 707
BstEII	GGTNACC	1 cut(s)	653
BstF5I	GGATG	1 cut(s)	1051
BstFNI	CGCG	5 cut(s)	72, 168, 211, 234, 1074
BstHHI	GCGC	1 cut(s)	170
BstKTI	GATC	5 cut(s)	81, 198, 322, 646, 1132
BstMAI	GTCTC	5 cut(s)	97, 200, 575, 737, 1156
BstMBI	GATC	5 cut(s)	78, 195, 319, 643, 1129
BstMCI	CGRYCG	2 cut(s)	70, 322
BstNI	CCWGG	3 cut(s)	126, 200, 382
BstPI	GGTNACC	1 cut(s)	653
BstSCI	CCNGG	3 cut(s)	124, 198, 380
BstSFI	CTRYAG	1 cut(s)	1105
BstSLI	GKGCMC	1 cut(s)	728
BstUI	CGCG	5 cut(s)	72, 168, 211, 234, 1074
BstV1I	GCAGC	8 cut(s)	153, 397, 414, 472, 487, 490, 493, 701
BstX2I	RGATCY	1 cut(s)	195
BstYI	RGATCY	1 cut(s)	195
BsuRI	GGCC	5 cut(s)	185, 246, 380, 520, 726
BtgI	CCRYGG	2 cut(s)	600, 707
BtgZI	GCGATG	1 cut(s)	1008
BtrI	CACGTC	1 cut(s)	1070
BtsCI	GGATG	1 cut(s)	1051
BtsI	GCAGTG	2 cut(s)	627, 690
BtsIMutI	CAGTG	3 cut(s)	627, 690, 1038
BveI	ACCTGC	3 cut(s)	379, 396, 572
Cac8I	GCNNGC	2 cut(s)	183, 879
CciI	TCATGA	1 cut(s)	646
CfoI	GCGC	1 cut(s)	170
Cfr13I	GGNCC	7 cut(s)	154, 163, 183, 519, 596, 724, 725
CseI	GACGC	4 cut(s)	59, 217, 587, 1119
Csp6I	GTAC	3 cut(s)	4, 339, 477
CviAII	CATG	5 cut(s)	76, 82, 334, 601, 647
CviQI	GTAC	3 cut(s)	4, 339, 477
DdeI	CTNAG	4 cut(s)	678, 729, 873, 1041
DpnI	GATC	5 cut(s)	80, 197, 321, 645, 1131
DpnII	GATC	5 cut(s)	78, 195, 319, 643, 1129
DraIII	CACNNNGTG	1 cut(s)	336
DrdI	GACNNNNNNGTC	1 cut(s)	161
DseDI	GACNNNNNNGTC	1 cut(s)	161
Eam1104I	CTCTTC	2 cut(s)	198, 254
EarI	CTCTTC	2 cut(s)	198, 254
EciI	GGCGGA	2 cut(s)	85, 218
Eco130I	CCWWGG	1 cut(s)	600
Eco24I	GRGCYC	2 cut(s)	329, 728
Eco47I	GGWCC	3 cut(s)	154, 163, 596
Eco88I	CYCGRG	2 cut(s)	292, 572
Eco91I	GGTNACC	1 cut(s)	653
EcoO109I	RGGNCCY	2 cut(s)	724, 725
EcoO65I	GGTNACC	1 cut(s)	653
EcoRII	CCWGG	3 cut(s)	124, 198, 380
EcoT14I	CCWWGG	1 cut(s)	600
EcoT38I	GRGCYC	2 cut(s)	329, 728
ErhI	CCWWGG	1 cut(s)	600
Esp3I	CGTCTC	2 cut(s)	97, 200
FaeI	CATG	5 cut(s)	79, 85, 337, 604, 650
FatI	CATG	5 cut(s)	75, 81, 333, 600, 646
FauI	CCCGC	1 cut(s)	174
FbaI	TGATCA	1 cut(s)	78
FblI	GTMKAC	1 cut(s)	48
FokI	GGATG	1 cut(s)	1058
FriOI	GRGCYC	2 cut(s)	329, 728
FspBI	CTAG	2 cut(s)	462, 1177
GlaI	GCGC	1 cut(s)	169
GsuI	CTGGAG	1 cut(s)	108
HaeIII	GGCC	5 cut(s)	185, 246, 380, 520, 726
HgaI	GACGC	4 cut(s)	59, 217, 587, 1119
HhaI	GCGC	1 cut(s)	170
Hin1I	GRCGYC	1 cut(s)	51
Hin1II	CATG	5 cut(s)	79, 85, 337, 604, 650
Hin6I	GCGC	1 cut(s)	168
HinP1I	GCGC	1 cut(s)	168
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HinfI	GANTC	4 cut(s)	542, 568, 916, 998
HphI	GGTGA	2 cut(s)	665, 1171
Hpy166II	GTNNAC	3 cut(s)	49, 339, 625
Hpy188I	TCNGA	7 cut(s)	13, 324, 534, 562, 863, 915, 1003
Hpy188III	TCNNGA	5 cut(s)	373, 572, 647, 920, 1055
Hpy8I	GTNNAC	3 cut(s)	49, 339, 625
Hpy99I	CGWCG	3 cut(s)	50, 53, 1074
HpyAV	CCTTC	4 cut(s)	370, 759, 798, 959
HpyCH4III	ACNGT	2 cut(s)	778, 1007
HpyCH4IV	ACGT	1 cut(s)	1069
HpyCH4V	TGCA	3 cut(s)	439, 857, 1145
HpyF3I	CTNAG	4 cut(s)	678, 729, 873, 1041
HpySE526I	ACGT	1 cut(s)	1069
Hsp92I	GRCGYC	1 cut(s)	51
Hsp92II	CATG	5 cut(s)	79, 85, 337, 604, 650
HspAI	GCGC	1 cut(s)	168
Ksp22I	TGATCA	1 cut(s)	78
Kzo9I	GATC	5 cut(s)	78, 195, 319, 643, 1129
LmnI	GCTCC	3 cut(s)	127, 283, 324
Lsp1109I	GCAGC	8 cut(s)	153, 397, 414, 472, 487, 490, 493, 701
LweI	GCATC	1 cut(s)	820
MaeI	CTAG	2 cut(s)	462, 1177
MaeII	ACGT	1 cut(s)	1069
MaeIII	GTNAC	1 cut(s)	653
MalI	GATC	5 cut(s)	80, 197, 321, 645, 1131
MbiI	CCGCTC	1 cut(s)	122
MboI	GATC	5 cut(s)	78, 195, 319, 643, 1129
MboII	GAAGA	5 cut(s)	187, 215, 241, 1034, 1124
MfeI	CAATTG	1 cut(s)	27
MflI	RGATCY	1 cut(s)	195
MhlI	GDGCHC	2 cut(s)	329, 728
MluCI	AATT	5 cut(s)	27, 523, 712, 943, 1094
MlyI	GAGTC	2 cut(s)	577, 925
MmeI	TCCRAC	3 cut(s)	291, 621, 870
MseI	TTAA	4 cut(s)	363, 837, 978, 1088
MslI	CAYNNNNRTG	3 cut(s)	80, 651, 960
MspA1I	CMGCKG	3 cut(s)	144, 500, 827
MspCI	CTTAAG	1 cut(s)	362
MspR9I	CCNGG	3 cut(s)	126, 200, 382
MunI	CAATTG	1 cut(s)	27
Mva1269I	GAATGC	1 cut(s)	857
MvaI	CCWGG	3 cut(s)	126, 200, 382
MvnI	CGCG	5 cut(s)	72, 168, 211, 234, 1074
NcoI	CCATGG	1 cut(s)	600
NdeII	GATC	5 cut(s)	78, 195, 319, 643, 1129
NlaIII	CATG	5 cut(s)	79, 85, 337, 604, 650
NlaIV	GGNNCC	6 cut(s)	197, 215, 326, 598, 702, 726
NmeAIII	GCCGAG	1 cut(s)	211
NmuCI	GTSAC	1 cut(s)	653
PaeR7I	CTCGAG	1 cut(s)	572
PagI	TCATGA	1 cut(s)	646
PctI	GAATGC	1 cut(s)	857
PfeI	GAWTC	2 cut(s)	542, 998
PfoI	TCCNGGA	1 cut(s)	198
Ple19I	CGATCG	1 cut(s)	322
PleI	GAGTC	2 cut(s)	576, 924
PpsI	GAGTC	2 cut(s)	576, 924
PsiI	TTATAA	2 cut(s)	513, 773
Psp6I	CCWGG	3 cut(s)	124, 198, 380
PspEI	GGTNACC	1 cut(s)	653
PspGI	CCWGG	3 cut(s)	124, 198, 380
PspN4I	GGNNCC	6 cut(s)	197, 215, 326, 598, 702, 726
PspOMI	GGGCCC	1 cut(s)	724
PspPI	GGNCC	7 cut(s)	154, 163, 183, 519, 596, 724, 725
PsuI	RGATCY	1 cut(s)	195
PvuI	CGATCG	1 cut(s)	322
PvuII	CAGCTG	1 cut(s)	827
RsaI	GTAC	3 cut(s)	5, 340, 478
RsaNI	GTAC	3 cut(s)	4, 339, 477
RseI	CAYNNNNRTG	3 cut(s)	80, 651, 960
SalI	GTCGAC	1 cut(s)	47
SaqAI	TTAA	4 cut(s)	363, 837, 978, 1088
Sau3AI	GATC	5 cut(s)	78, 195, 319, 643, 1129
Sau96I	GGNCC	7 cut(s)	154, 163, 183, 519, 596, 724, 725
SchI	GAGTC	2 cut(s)	577, 925
ScrFI	CCNGG	3 cut(s)	126, 200, 382
SduI	GDGCHC	2 cut(s)	329, 728
SfaNI	GCATC	1 cut(s)	820
SfcI	CTRYAG	1 cut(s)	1105
Sfr274I	CTCGAG	1 cut(s)	572
SgrDI	CGTCGACG	1 cut(s)	47
SinI	GGWCC	3 cut(s)	154, 163, 596
SlaI	CTCGAG	1 cut(s)	572
SmiMI	CAYNNNNRTG	3 cut(s)	80, 651, 960
SmlI	CTYRAG	2 cut(s)	362, 572
SmoI	CTYRAG	2 cut(s)	362, 572
Sse9I	AATT	5 cut(s)	27, 523, 712, 943, 1094
SspMI	CTAG	2 cut(s)	462, 1177
StyD4I	CCNGG	3 cut(s)	124, 198, 380
StyI	CCWWGG	1 cut(s)	600
TaaI	ACNGT	2 cut(s)	778, 1007
TaiI	ACGT	1 cut(s)	1072
TaqI	TCGA	5 cut(s)	48, 240, 318, 540, 573
TaqII	GACCGA	1 cut(s)	627
TasI	AATT	5 cut(s)	27, 523, 712, 943, 1094
TfiI	GAWTC	2 cut(s)	542, 998
Tru1I	TTAA	4 cut(s)	363, 837, 978, 1088
Tru9I	TTAA	4 cut(s)	363, 837, 978, 1088
TscAI	CASTG	3 cut(s)	627, 690, 1045
TseFI	GTSAC	1 cut(s)	653
TseI	GCWGC	8 cut(s)	141, 385, 402, 485, 500, 503, 506, 689
Tsp45I	GTSAC	1 cut(s)	653
TspDTI	ATGAA	3 cut(s)	538, 978, 1011
TspGWI	ACGGA	4 cut(s)	34, 356, 579, 696
TspRI	CASTG	3 cut(s)	627, 690, 1045
Vha464I	CTTAAG	1 cut(s)	362
VpaK11BI	GGWCC	3 cut(s)	154, 163, 596
XhoI	CTCGAG	1 cut(s)	572
XmiI	GTMKAC	1 cut(s)	48
XspI	CTAG	2 cut(s)	462, 1177

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.