Rw5G025200

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007592
Physical Location & Seq
Reverse (-)
35872310 .. 35876680
4371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025200.1

Sequence Viewer

Length: 303 bp
ATGAACAACTTTTTCCAGAAGAGTATTACTTTGAAGCCTGAAGAAGTTACTGGCATTGTGAATGACTTTAATGAACCTGGAACACTTGCTCCAACTGGGTTGTATCTTGGAGGCACTAAGTACATGGTGATCCAAGGAGAGCCTGGAGCTGTCATTAGAGGGAAGAAGGGCCCTGGAGGTGTTACTGTGAAGAAGACTACTCTGGCATTGCTCATTGGGATCTATGATGAGCCAATGACTCCCGAACAATGCAACATGATTGTGGAGAGGCTTGGCGATTATCTGGTTGAGCAGGGTCTCTAG

Protein Analysis

100

Amino Acids

10.82

Weight (kDa)

5.02

Isoelectric Point (pI)

18.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Profilin PF00235 7 - 100 2.3e-28 Profilin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 124, 227
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 1 cut(s) 122
AgsI TTSAA 1 cut(s) 34
AjnI CCWGG 3 cut(s) 76, 142, 172
AloI GAACNNNNNNTCC 2 cut(s) 73, 105
AluBI AGCT 1 cut(s) 149
AluI AGCT 1 cut(s) 149
AlwI GGATC 2 cut(s) 124, 227
AoxI GGCC 1 cut(s) 169
ApaI GGGCCC 1 cut(s) 173
AspS9I GGNCC 2 cut(s) 169, 170
AsuHPI GGTGA 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 200
BciT130I CCWGG 3 cut(s) 78, 144, 174
BfaI CTAG 1 cut(s) 301
Bme1390I CCNGG 3 cut(s) 78, 144, 174
BmgT120I GGNCC 2 cut(s) 169, 170
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 3 cut(s) 78, 144, 174
BmrI ACTGGG 1 cut(s) 105
BmuI ACTGGG 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 200
BpmI CTGGAG 2 cut(s) 165, 195
BsaJI CCNNGG 2 cut(s) 133, 172
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bse1I ACTGG 2 cut(s) 55, 100
Bse3DI GCAATG 1 cut(s) 206
BseBI CCWGG 3 cut(s) 78, 144, 174
BseDI CCNNGG 2 cut(s) 133, 172
BseMI GCAATG 1 cut(s) 206
BseNI ACTGG 2 cut(s) 55, 100
BseSI GKGCMC 1 cut(s) 173
BshFI GGCC 1 cut(s) 171
BsnI GGCC 1 cut(s) 171
Bsp120I GGGCCC 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 2 cut(s) 129, 219
BspANI GGCC 1 cut(s) 171
BspLI GGNNCC 1 cut(s) 171
BspPI GGATC 2 cut(s) 124, 227
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 2 cut(s) 55, 100
BssECI CCNNGG 2 cut(s) 133, 172
BssMI GATC 2 cut(s) 129, 219
BssT1I CCWWGG 1 cut(s) 133
Bst2UI CCWGG 3 cut(s) 78, 144, 174
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 14
BstDEI CTNAG 1 cut(s) 117
BstKTI GATC 2 cut(s) 132, 222
BstMBI GATC 2 cut(s) 129, 219
BstNI CCWGG 3 cut(s) 78, 144, 174
BstSCI CCNGG 3 cut(s) 76, 142, 172
BstSLI GKGCMC 1 cut(s) 173
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BsuRI GGCC 1 cut(s) 171
Cfr13I GGNCC 2 cut(s) 169, 170
Csp6I GTAC 1 cut(s) 121
CviAII CATG 2 cut(s) 124, 256
CviJI RGCY 6 cut(s) 37, 142, 149, 171, 232, 271
CviKI_1 RGCY 6 cut(s) 37, 142, 149, 171, 232, 271
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 1 cut(s) 117
DpnI GATC 2 cut(s) 131, 221
DpnII GATC 2 cut(s) 129, 219
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 60
EcoO109I RGGNCCY 2 cut(s) 169, 170
EcoRII CCWGG 3 cut(s) 76, 142, 172
EcoT14I CCWWGG 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 2 cut(s) 127, 259
FaiI YATR 3 cut(s) 125, 225, 257
FatI CATG 2 cut(s) 123, 255
FriOI GRGCYC 1 cut(s) 173
FspBI CTAG 1 cut(s) 301
GsuI CTGGAG 2 cut(s) 165, 195
HaeIII GGCC 1 cut(s) 171
Hin1II CATG 2 cut(s) 127, 259
HinfI GANTC 1 cut(s) 238
HphI GGTGA 1 cut(s) 139
Hpy188III TCNNGA 2 cut(s) 16, 242
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4V TGCA 1 cut(s) 252
HpyF3I CTNAG 1 cut(s) 117
Hsp92II CATG 2 cut(s) 127, 259
Kzo9I GATC 2 cut(s) 129, 219
LmnI GCTCC 2 cut(s) 94, 146
MaeI CTAG 1 cut(s) 301
MaeIII GTNAC 2 cut(s) 46, 181
MalI GATC 2 cut(s) 131, 221
MboI GATC 2 cut(s) 129, 219
MboII GAAGA 5 cut(s) 31, 53, 175, 202, 205
MflI RGATCY 1 cut(s) 219
MhlI GDGCHC 1 cut(s) 173
MlyI GAGTC 1 cut(s) 232
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 4 cut(s) 104, 152, 170, 261
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 260
MspR9I CCNGG 3 cut(s) 78, 144, 174
MvaI CCWGG 3 cut(s) 78, 144, 174
NdeII GATC 2 cut(s) 129, 219
NlaIII CATG 2 cut(s) 127, 259
NlaIV GGNNCC 1 cut(s) 171
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Psp6I CCWGG 3 cut(s) 76, 142, 172
PspGI CCWGG 3 cut(s) 76, 142, 172
PspN4I GGNNCC 1 cut(s) 171
PspOMI GGGCCC 1 cut(s) 169
PspPI GGNCC 2 cut(s) 169, 170
PsuI RGATCY 1 cut(s) 219
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 69
Sau3AI GATC 2 cut(s) 129, 219
Sau96I GGNCC 2 cut(s) 169, 170
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 3 cut(s) 78, 144, 174
SduI GDGCHC 1 cut(s) 173
SetI ASST 3 cut(s) 79, 151, 181
SmiMI CAYNNNNRTG 1 cut(s) 260
SspMI CTAG 1 cut(s) 301
StyD4I CCNGG 3 cut(s) 76, 142, 172
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 1 cut(s) 187
TatI WGTACW 1 cut(s) 120
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TspDTI ATGAA 2 cut(s) 17, 87
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XspI CTAG 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.