Rw5G037730

DDB1- and CUL4-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010743
Physical Location & Seq
Reverse (-)
66392333 .. 66401747
9415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037730.1

Sequence Viewer

Length: 945 bp
ATGTATGGGGAATTAAAAGGGCATAACGATTATGTAAGCTCCATAGAATTCAACTCCACTGGTGACCTCCTTGTGTCAGGGTCTTATGATAAACAAGTCATATTTTGGAACTGGGAAACTAAAACTAGAAGACTCTCTTATGATTCTGGACACTTGAAGATGGTATATCAGACAAAGATAATGCCTCTCCCGAATGATCGGAGAATAGTAACCGCTTGTGCAGTTGGTCAGGTGAGGCTAGGTGAGGTCTTAGAGGATGGCCAAGTATTGGGGAGTCACAGAGGCGCTGTTCACAAGCTGGCTGTAGAGCCTGGAAACCCCAACATACTTTGCGGCTGTGAGCAGCACCCTTTCATGTTAGATTTGTATGACATGGTGATTGACCCATGGAATCCAAACTACTTTTGTCTAGGAGGAAATGATGCATATGCACGCGTCTATGACATGAGAAAATGCTGGCAGAATGCGTCAGGTGGTTTAGTTAAACCTGTGAACACATTTCGCCCTGCTCACCTGATTCAGACAACCCATTTTATGATCACAGGTTATAGTGATGAGCACATTTATCTGTTCCAGAAGAATATGGGGTTGGAACAATTCGTTGGTCATAGAAATATAAACACATGTAGAGTGAGTTTCTTTGGGCCAAATGATGAGTATGTCTTGAGTGGGAGTTATTGCGGCCATATTTTCATATGGAAGAAGAATGGAGGTGAACTTATTCGTGTTATGATGGGCGATTCTCTAGGTGTACATGACATTCAGCCGCATCCACATAATCCGGTCATTGCCACCGGCGGATCGGGAAACAATCTAAAGCTCTGGACTCCTATGGCAAATGATCATGCATCTCCACTGCCTGACAACATTGCACAGATTATGGAGTCTAATAGTCGAGCTAGAAGTGATGATTATTTTCAGATGCTCACATGGAGTTCCTCCTGA

Protein Analysis

314

Amino Acids

35.42

Weight (kDa)

6.25

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40 PF00400 4 - 37 2.6e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 4 - 80 6.6e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 5 - 116 4.2e-10 WDR3 first beta-propeller domain
EIF3I PF24805 5 - 92 5e-06 EIF3I
Beta-prop_THOC3 PF25174 8 - 80 1.7e-07 THOC3 beta-propeller domain
WD40_Prp19 PF24814 126 - 286 3.7e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 139 - 275 3.7e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 268
AccII CGCG 1 cut(s) 435
AciI CCGC 5 cut(s) 213, 333, 681, 767, 798
AclWI GGATC 1 cut(s) 808
AcoI YGGCCR 2 cut(s) 259, 682
AcsI RAATTY 1 cut(s) 47
AfaI GTAC 1 cut(s) 753
AfiI CCNNNNNNNGG 1 cut(s) 268
AflIII ACRYGT 2 cut(s) 433, 623
AgsI TTSAA 2 cut(s) 52, 157
AjnI CCWGG 1 cut(s) 310
AjuI GAANNNNNNNTTGG 4 cut(s) 572, 585, 604, 617
AluBI AGCT 4 cut(s) 39, 298, 820, 899
AluI AGCT 4 cut(s) 39, 298, 820, 899
Alw21I GWGCWC 1 cut(s) 561
AlwI GGATC 1 cut(s) 808
AoxI GGCC 3 cut(s) 259, 644, 682
ApeKI GCWGC 1 cut(s) 343
ApoI RAATTY 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 1 cut(s) 644
AsuHPI GGTGA 6 cut(s) 74, 244, 254, 388, 503, 725
BalI TGGCCA 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 355
BccI CCATC 3 cut(s) 154, 251, 727
BciT130I CCWGG 1 cut(s) 312
BclI TGATCA 2 cut(s) 537, 841
BfaI CTAG 5 cut(s) 126, 239, 410, 746, 900
BfmI CTRYAG 1 cut(s) 303
BfoI RGCGCY 1 cut(s) 288
BisI GCNGC 4 cut(s) 334, 344, 682, 767
BlsI GCNGC 4 cut(s) 335, 345, 683, 768
Bme1390I CCNGG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 644
BmrFI CCNGG 1 cut(s) 312
BmrI ACTGGG 1 cut(s) 121
BmsI GCATC 4 cut(s) 412, 778, 857, 912
BmuI ACTGGG 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 136
BpuEI CTTGAG 1 cut(s) 685
BsaJI CCNNGG 1 cut(s) 386
BsaWI WCCGGW 1 cut(s) 781
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse118I RCCGGY 1 cut(s) 794
Bse1I ACTGG 2 cut(s) 64, 116
Bse3DI GCAATG 2 cut(s) 786, 867
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 2 cut(s) 262, 769
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMI GCAATG 2 cut(s) 786, 867
BseNI ACTGG 2 cut(s) 64, 116
BseXI GCAGC 1 cut(s) 355
BsgI GTGCAG 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 3 cut(s) 261, 646, 684
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 2 cut(s) 782, 795
BslI CCNNNNNNNGG 1 cut(s) 268
BsmI GAATGC 1 cut(s) 469
BsnI GGCC 3 cut(s) 261, 646, 684
Bsp1286I GDGCHC 1 cut(s) 561
Bsp1407I TGTACA 1 cut(s) 751
Bsp143I GATC 4 cut(s) 196, 537, 800, 841
Bsp19I CCATGG 1 cut(s) 386
BspACI CCGC 5 cut(s) 213, 333, 681, 767, 798
BspANI GGCC 3 cut(s) 261, 646, 684
BspFNI CGCG 1 cut(s) 435
BspPI GGATC 1 cut(s) 808
BsrDI GCAATG 2 cut(s) 786, 867
BsrFI RCCGGY 1 cut(s) 794
BsrGI TGTACA 1 cut(s) 751
BsrI ACTGG 2 cut(s) 64, 116
BssAI RCCGGY 1 cut(s) 794
BssECI CCNNGG 1 cut(s) 386
BssMI GATC 4 cut(s) 196, 537, 800, 841
BssT1I CCWWGG 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 312
BstAUI TGTACA 1 cut(s) 751
BstC8I GCNNGC 3 cut(s) 300, 433, 458
BstDEI CTNAG 1 cut(s) 250
BstDSI CCRYGG 1 cut(s) 386
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 2 cut(s) 262, 769
BstFNI CGCG 1 cut(s) 435
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 4 cut(s) 199, 540, 803, 844
BstMBI GATC 4 cut(s) 196, 537, 800, 841
BstNI CCWGG 1 cut(s) 312
BstNSI RCATGY 1 cut(s) 627
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 355
BstV2I GAAGAC 1 cut(s) 136
BsuRI GGCC 3 cut(s) 261, 646, 684
BtgI CCRYGG 1 cut(s) 386
BtsCI GGATG 2 cut(s) 262, 769
BtsI GCAGTG 1 cut(s) 854
BtsIMutI CAGTG 2 cut(s) 57, 854
Cac8I GCNNGC 3 cut(s) 300, 433, 458
CfoI GCGC 1 cut(s) 287
Cfr10I RCCGGY 1 cut(s) 794
Cfr13I GGNCC 1 cut(s) 644
CseI GACGC 2 cut(s) 424, 456
Csp6I GTAC 1 cut(s) 752
CviAII CATG 8 cut(s) 355, 373, 387, 445, 624, 755, 845, 930
CviQI GTAC 1 cut(s) 752
DdeI CTNAG 1 cut(s) 250
DpnI GATC 4 cut(s) 198, 539, 802, 843
DpnII GATC 4 cut(s) 196, 537, 800, 841
EaeI YGGCCR 2 cut(s) 259, 682
EciI GGCGGA 1 cut(s) 813
Eco130I CCWWGG 1 cut(s) 386
Eco91I GGTNACC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 386
EcoT22I ATGCAT 2 cut(s) 427, 850
ErhI CCWWGG 1 cut(s) 386
FaeI CATG 8 cut(s) 358, 376, 390, 448, 627, 758, 848, 933
FalI AAGNNNNNCTT 2 cut(s) 121, 153
FatI CATG 8 cut(s) 354, 372, 386, 444, 623, 754, 844, 929
FauNDI CATATG 2 cut(s) 427, 695
FbaI TGATCA 2 cut(s) 537, 841
Fnu4HI GCNGC 4 cut(s) 334, 344, 682, 767
FokI GGATG 2 cut(s) 269, 756
Fsp4HI GCNGC 4 cut(s) 334, 344, 682, 767
FspBI CTAG 5 cut(s) 126, 239, 410, 746, 900
GlaI GCGC 1 cut(s) 286
GluI GCNGC 4 cut(s) 334, 344, 682, 767
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 3 cut(s) 261, 646, 684
HapII CCGG 2 cut(s) 782, 795
HgaI GACGC 2 cut(s) 424, 456
HhaI GCGC 1 cut(s) 287
Hin1II CATG 8 cut(s) 358, 376, 390, 448, 627, 758, 848, 933
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 8 cut(s) 132, 143, 274, 391, 517, 740, 826, 884
HpaII CCGG 2 cut(s) 782, 795
HphI GGTGA 6 cut(s) 74, 244, 254, 388, 503, 725
Hpy166II GTNNAC 4 cut(s) 292, 493, 716, 752
Hpy188I TCNGA 4 cut(s) 171, 201, 522, 921
Hpy188III TCNNGA 7 cut(s) 147, 190, 574, 664, 804, 823, 942
Hpy8I GTNNAC 4 cut(s) 292, 493, 716, 752
HpyCH4V TGCA 5 cut(s) 221, 425, 431, 848, 872
HpyF3I CTNAG 1 cut(s) 250
Hsp92II CATG 8 cut(s) 358, 376, 390, 448, 627, 758, 848, 933
HspAI GCGC 1 cut(s) 285
Ksp22I TGATCA 2 cut(s) 537, 841
Kzo9I GATC 4 cut(s) 196, 537, 800, 841
LmnI GCTCC 1 cut(s) 44
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 4 cut(s) 412, 778, 857, 912
MaeI CTAG 5 cut(s) 126, 239, 410, 746, 900
MaeIII GTNAC 3 cut(s) 62, 208, 275
MalI GATC 4 cut(s) 198, 539, 802, 843
MboI GATC 4 cut(s) 196, 537, 800, 841
MboII GAAGA 5 cut(s) 141, 169, 589, 712, 715
MhlI GDGCHC 1 cut(s) 561
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 3 cut(s) 11, 47, 596
MluI ACGCGT 1 cut(s) 433
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 4 cut(s) 126, 283, 820, 893
MmeI TCCRAC 1 cut(s) 570
MnlI CCTC 8 cut(s) 77, 195, 228, 238, 247, 275, 407, 704
Mox20I TGGCCA 1 cut(s) 261
Mph1103I ATGCAT 2 cut(s) 427, 850
MroXI GAANNNNTTC 1 cut(s) 720
MscI TGGCCA 1 cut(s) 261
MseI TTAA 2 cut(s) 14, 483
Msp20I TGGCCA 1 cut(s) 261
MspI CCGG 2 cut(s) 782, 795
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 469
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 1 cut(s) 435
NcoI CCATGG 1 cut(s) 386
NdeI CATATG 2 cut(s) 427, 695
NdeII GATC 4 cut(s) 196, 537, 800, 841
NlaIII CATG 8 cut(s) 358, 376, 390, 448, 627, 758, 848, 933
NmuCI GTSAC 2 cut(s) 62, 275
NsiI ATGCAT 2 cut(s) 427, 850
NspI RCATGY 1 cut(s) 627
PciI ACATGT 1 cut(s) 623
PctI GAATGC 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 4 cut(s) 143, 391, 517, 740
PflMI CCANNNNNTGG 1 cut(s) 268
PkrI GCNGC 4 cut(s) 335, 345, 683, 768
PleI GAGTC 4 cut(s) 126, 282, 820, 892
PpsI GAGTC 4 cut(s) 126, 282, 820, 892
PscI ACATGT 1 cut(s) 623
Psp6I CCWGG 1 cut(s) 310
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 310
PspPI GGNCC 1 cut(s) 644
RsaI GTAC 1 cut(s) 753
RsaNI GTAC 1 cut(s) 752
SaqAI TTAA 2 cut(s) 14, 483
SatI GCNGC 4 cut(s) 334, 344, 682, 767
Sau3AI GATC 4 cut(s) 196, 537, 800, 841
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 4 cut(s) 126, 283, 820, 893
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 4 cut(s) 412, 778, 857, 912
SfcI CTRYAG 1 cut(s) 303
SgrAI CRCCGGYG 1 cut(s) 794
SmlI CTYRAG 1 cut(s) 664
SmoI CTYRAG 1 cut(s) 664
Sse9I AATT 3 cut(s) 11, 47, 596
SsiI CCGC 5 cut(s) 213, 333, 681, 767, 798
SspMI CTAG 5 cut(s) 126, 239, 410, 746, 900
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 1 cut(s) 386
TaqI TCGA 1 cut(s) 895
TasI AATT 3 cut(s) 11, 47, 596
TatI WGTACW 1 cut(s) 751
TauI GCSGC 3 cut(s) 336, 684, 769
TfiI GAWTC 4 cut(s) 143, 391, 517, 740
Tru1I TTAA 2 cut(s) 14, 483
Tru9I TTAA 2 cut(s) 14, 483
TscAI CASTG 2 cut(s) 64, 861
TseFI GTSAC 2 cut(s) 62, 275
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 2 cut(s) 62, 275
TspDTI ATGAA 2 cut(s) 343, 682
TspRI CASTG 2 cut(s) 64, 861
Van91I CCANNNNNTGG 1 cut(s) 268
XapI RAATTY 1 cut(s) 47
XceI RCATGY 1 cut(s) 627
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 5 cut(s) 126, 239, 410, 746, 900
Zsp2I ATGCAT 2 cut(s) 427, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.