Rw6G003910

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008362
Physical Location & Seq
Forward (+)
6383115 .. 6385251
2137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003910.1

Sequence Viewer

Length: 627 bp
ATGGCTTCTCTTCAAATCTTCAAGTCCACCCTCTCTCCCAGCCCTGTTTCCCAGTCAAGTTTCACCGGAAAATCCAGGTGGGTCTTGTCGGAAATTCAGTCCAGGAATAACAGTATCATCAGGCTCTCTGGTTATTATTACTTGAGGAGGAGGAGGTTCAGGGTCTGCTGCAAAGCTCAAGAATCAGATAACAAAAGCAATGGGGAAGAACCGCCCGAGTCATTGTTTATGAAGGAATTGAGGAGGAGGGGTATGAGTCCGACTTCTTTGCTTGAGGAAGCAGAGAGGACCGAGCATGGAATCAATGATGAGAGGGGCAAAGGTGATAGAGGTTTCTCAAATAGAAATGCGGTCTCAACTGAAGTGGAGAAAAGCCTGGCTAATCAAAGGGAGCGATCCATGCAGTTGAACAGTGAAGGCCTCGAGGGGTTGATCCCTCGAGGAAGACTCTTGCTGACGCTTGGAGGGACATTCTTCTTGGCATTTTGGCCATTTATGGTCATAAGTTACTTTGGACCAACATTTATCCATGATGCAAACAACAGAATATCACCACCTCAATACATTGATCCGTATGAACTTCTGGAAGATGATAGGATTTCTCAAATAGCCCCTCGTGTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

23.83

Weight (kDa)

9.35

Isoelectric Point (pI)

66.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 2 cut(s) 422, 438
AciI CCGC 2 cut(s) 212, 350
AclWI GGATC 3 cut(s) 390, 427, 563
AcoI YGGCCR 1 cut(s) 488
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 381
AgsI TTSAA 3 cut(s) 14, 22, 409
AjnI CCWGG 3 cut(s) 74, 101, 375
AluBI AGCT 1 cut(s) 176
AluI AGCT 1 cut(s) 176
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 3 cut(s) 390, 427, 563
AlwNI CAGNNNCTG 1 cut(s) 165
Ama87I CYCGRG 3 cut(s) 215, 422, 438
AoxI GGCC 2 cut(s) 418, 488
ApeKI GCWGC 1 cut(s) 168
ApoI RAATTY 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 288, 515
AsuHPI GGTGA 3 cut(s) 55, 335, 543
AvaI CYCGRG 3 cut(s) 215, 422, 438
AvaII GGWCC 2 cut(s) 288, 515
BalI TGGCCA 1 cut(s) 490
BauI CACGAG 1 cut(s) 615
BbsI GAAGAC 1 cut(s) 451
BbvI GCAGC 1 cut(s) 155
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 3 cut(s) 76, 103, 377
BcoDI GTCTC 1 cut(s) 358
BisI GCNGC 1 cut(s) 169
BlsI GCNGC 1 cut(s) 170
Bme1390I CCNGG 3 cut(s) 76, 103, 377
Bme18I GGWCC 2 cut(s) 288, 515
BmeT110I CYCGRG 3 cut(s) 215, 422, 438
BmgT120I GGNCC 2 cut(s) 288, 515
BmrFI CCNGG 3 cut(s) 76, 103, 377
BmrI ACTGGG 1 cut(s) 46
BmsI GCATC 1 cut(s) 523
BmuI ACTGGG 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 451
BplI GAGNNNNNCTC 2 cut(s) 432, 464
BpuEI CTTGAG 3 cut(s) 162, 163, 293
BsaI GGTCTC 1 cut(s) 358
BsaWI WCCGGW 1 cut(s) 65
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 235, 265
Bse1I ACTGG 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 205
BseBI CCWGG 3 cut(s) 76, 103, 377
BseMI GCAATG 1 cut(s) 205
BseNI ACTGG 1 cut(s) 52
BseRI GAGGAG 5 cut(s) 160, 163, 166, 256, 259
BseXI GCAGC 1 cut(s) 155
BseYI CCCAGC 1 cut(s) 38
BshFI GGCC 2 cut(s) 420, 490
BsiHKCI CYCGRG 3 cut(s) 215, 422, 438
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 1 cut(s) 481
BsmAI GTCTC 1 cut(s) 358
BsmFI GGGAC 1 cut(s) 481
BsnI GGCC 2 cut(s) 420, 490
Bso31I GGTCTC 1 cut(s) 358
BsoBI CYCGRG 3 cut(s) 215, 422, 438
Bsp143I GATC 3 cut(s) 395, 432, 568
BspACI CCGC 2 cut(s) 212, 350
BspANI GGCC 2 cut(s) 420, 490
BspPI GGATC 3 cut(s) 390, 427, 563
BspTNI GGTCTC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 205
BsrI ACTGG 1 cut(s) 52
BssMI GATC 3 cut(s) 395, 432, 568
BssSI CACGAG 1 cut(s) 615
Bst2BI CACGAG 1 cut(s) 615
Bst2UI CCWGG 3 cut(s) 76, 103, 377
Bst4CI ACNGT 2 cut(s) 113, 413
Bst6I CTCTTC 1 cut(s) 15
BstKTI GATC 3 cut(s) 398, 435, 571
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 3 cut(s) 395, 432, 568
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNI CCWGG 3 cut(s) 76, 103, 377
BstSCI CCNGG 3 cut(s) 74, 101, 375
BstV1I GCAGC 1 cut(s) 155
BstV2I GAAGAC 1 cut(s) 451
BsuRI GGCC 2 cut(s) 420, 490
BtsIMutI CAGTG 1 cut(s) 418
CaiI CAGNNNCTG 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 288, 515
CseI GACGC 1 cut(s) 466
CspCI CAANNNNNGTGG 2 cut(s) 345, 380
CviAII CATG 3 cut(s) 296, 400, 530
CviJI RGCY 9 cut(s) 5, 42, 124, 176, 375, 380, 420, 490, 611
CviKI_1 RGCY 9 cut(s) 5, 42, 124, 176, 375, 380, 420, 490, 611
DpnI GATC 3 cut(s) 397, 434, 570
DpnII GATC 3 cut(s) 395, 432, 568
EaeI YGGCCR 1 cut(s) 488
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
Eco147I AGGCCT 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 358
Eco47I GGWCC 2 cut(s) 288, 515
Eco57I CTGAAG 1 cut(s) 381
Eco88I CYCGRG 3 cut(s) 215, 422, 438
EcoRII CCWGG 3 cut(s) 74, 101, 375
FaeI CATG 3 cut(s) 299, 403, 533
FaiI YATR 8 cut(s) 230, 254, 297, 401, 497, 503, 531, 576
FaqI GGGAC 1 cut(s) 481
FatI CATG 3 cut(s) 295, 399, 529
Fnu4HI GCNGC 1 cut(s) 169
Fsp4HI GCNGC 1 cut(s) 169
GluI GCNGC 1 cut(s) 169
GsaI CCCAGC 1 cut(s) 42
HaeIII GGCC 2 cut(s) 420, 490
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 466
Hin1II CATG 3 cut(s) 299, 403, 533
HinfI GANTC 5 cut(s) 182, 218, 256, 300, 447
HpaII CCGG 1 cut(s) 66
HphI GGTGA 3 cut(s) 55, 335, 543
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 3 cut(s) 91, 187, 261
Hpy188III TCNNGA 2 cut(s) 179, 584
Hpy8I GTNNAC 1 cut(s) 27
HpyAV CCTTC 2 cut(s) 226, 410
HpyCH4III ACNGT 2 cut(s) 113, 413
HpyCH4V TGCA 3 cut(s) 171, 403, 536
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
Hsp92II CATG 3 cut(s) 299, 403, 533
Kzo9I GATC 3 cut(s) 395, 432, 568
LmnI GCTCC 1 cut(s) 391
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 1 cut(s) 523
MaeIII GTNAC 1 cut(s) 506
MalI GATC 3 cut(s) 397, 434, 570
MboI GATC 3 cut(s) 395, 432, 568
MboII GAAGA 5 cut(s) 10, 218, 456, 466, 599
MlsI TGGCCA 1 cut(s) 490
MluCI AATT 2 cut(s) 93, 236
MluNI TGGCCA 1 cut(s) 490
MlyI GAGTC 3 cut(s) 227, 265, 441
MmeI TCCRAC 2 cut(s) 69, 284
Mox20I TGGCCA 1 cut(s) 490
MscI TGGCCA 1 cut(s) 490
Msp20I TGGCCA 1 cut(s) 490
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 3 cut(s) 76, 103, 377
MvaI CCWGG 3 cut(s) 76, 103, 377
MwoI GCNNNNNNNGC 1 cut(s) 400
NdeII GATC 3 cut(s) 395, 432, 568
NlaIII CATG 3 cut(s) 299, 403, 533
PaeR7I CTCGAG 2 cut(s) 422, 438
PceI AGGCCT 1 cut(s) 420
PfeI GAWTC 2 cut(s) 182, 300
PfoI TCCNGGA 1 cut(s) 101
PkrI GCNGC 1 cut(s) 170
PleI GAGTC 3 cut(s) 226, 264, 441
PpsI GAGTC 3 cut(s) 226, 264, 441
Psp6I CCWGG 3 cut(s) 74, 101, 375
PspFI CCCAGC 1 cut(s) 38
PspGI CCWGG 3 cut(s) 74, 101, 375
PspPI GGNCC 2 cut(s) 288, 515
PspXI VCTCGAGB 2 cut(s) 422, 438
PstNI CAGNNNCTG 1 cut(s) 165
SatI GCNGC 1 cut(s) 169
Sau3AI GATC 3 cut(s) 395, 432, 568
Sau96I GGNCC 2 cut(s) 288, 515
SchI GAGTC 3 cut(s) 227, 265, 441
ScrFI CCNGG 3 cut(s) 76, 103, 377
SetI ASST 6 cut(s) 80, 158, 178, 325, 334, 559
SfaNI GCATC 1 cut(s) 523
Sfr274I CTCGAG 2 cut(s) 422, 438
SinI GGWCC 2 cut(s) 288, 515
SlaI CTCGAG 2 cut(s) 422, 438
SmlI CTYRAG 5 cut(s) 142, 177, 272, 422, 438
SmoI CTYRAG 5 cut(s) 142, 177, 272, 422, 438
Sse9I AATT 2 cut(s) 93, 236
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 2 cut(s) 212, 350
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 3 cut(s) 74, 101, 375
TaaI ACNGT 2 cut(s) 113, 413
TaqI TCGA 2 cut(s) 423, 439
TaqII GACCGA 1 cut(s) 305
TasI AATT 2 cut(s) 93, 236
TfiI GAWTC 2 cut(s) 182, 300
TscAI CASTG 1 cut(s) 418
TseI GCWGC 1 cut(s) 168
TspDTI ATGAA 2 cut(s) 245, 591
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 1 cut(s) 418
VpaK11BI GGWCC 2 cut(s) 288, 515
XapI RAATTY 1 cut(s) 93
XhoI CTCGAG 2 cut(s) 422, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.