Rw7G019570

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007437
Physical Location & Seq
Forward (+)
21731648 .. 21732843
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G019570.1

Sequence Viewer

Length: 1167 bp
ATGACATTAAGGTGCAGGAAAAGACACATAAGCTGCTGGAAAATAACATTAAGGTGCAGGAAAAGACACTTCAGCTTGCATTTCAAGTTTAATGATGTGGTTGCTGCCAGTGAGCTTATATTACAAATGTGTGATGATCGCAAGTCTTTTCCAATTCAGAGAGACAAGAAGAACTCACAAAGGTCTTATCTTGTTCCAATTGGATCTCATAATCTGAAGTCTGGGTTGAATATGCAGATTTTCCCTGGGCTGCTGCAGAAAGATTCTGTCCTCAAATTAGAAGGTAAACAAGAGCTTGTTATGTATATGAATGGGAAACTTGTTCTTAGCAACAGAGCTCTGGCCAAGATTATAACCAGACACAAAAAGGATGGGGATATTTCCGAGCTTTCAAAACTTTTACTCAAAATTCAAAAGGATTTATGTTCATTAAGAGGATCCAGATTGGGCAATGATGTGATTCACGCTTGCATTCATTTAGATGACATGGAAACAGCTGAGGCTCCCATGGGCTATAGTACCTGTATGTCACTCCTGACAGCCTATTACAAAAGAAAAATGTTTCCAGAAGCAAAGGCATTGCTTAAACAGATGAGAAAGGCTGGTTTGCTTGTAAATTTATCTGATGAGATGGTTGCCTCGACATGTCTATCAGTAGTAGACTCAACTGCCCTTTGTACAAGTGCATCAATGCCCACCGGTAAATCTGATCTGGCAAATGCTTTGGTTCAGGAAATGAGAGATGAGAAAGAGGAAATTCCTTGTAGGGTTTATCAGTTCAATTCTTCCATCAATTTTTTTTGCAAGGCCAAAATGATTGATGATGCTCTAAAGACATACAAAAGAATGCAGGAGTTGAAAATTCACCCCACTGAGGAAACTTTTACCTATATGATAAATGTATATTCTTCTCTAGGAATGTTCCGTAATATCACATTCTTGTGGGGGGACATGAAGAGGAATATGGATAATGGAAATTTGGTGGTAAGCAGGGATCTGTATGAGTACCTGCTACTGAACTTTCTCCGTGGTGGTTACTTTGAGAGAGTCATGGAGGTCATTAACTATATGAAGGAGCACAGTATGTTTGCTGACAAGTGGATGTACAGAAGTGAGTTTTTAAAGCTTCATAAGAATCTTTATAGGAACTTGAAGGCATCAGAATAG

Protein Analysis

388

Amino Acids

45.05

Weight (kDa)

9.39

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 260 - 302 1.8e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 1017
AccI GTMKAC 1 cut(s) 660
AclWI GGATC 4 cut(s) 211, 432, 445, 1002
AcoI YGGCCR 1 cut(s) 342
AcsI RAATTY 5 cut(s) 408, 616, 756, 861, 976
AcuI CTGAAG 2 cut(s) 55, 236
AfaI GTAC 4 cut(s) 520, 679, 1007, 1106
AfiI CCNNNNNNNGG 1 cut(s) 874
AflIII ACRYGT 1 cut(s) 644
AgeI ACCGGT 1 cut(s) 698
AgsI TTSAA 7 cut(s) 85, 229, 393, 413, 781, 859, 1153
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 8 cut(s) 33, 75, 115, 295, 338, 388, 497, 1126
AluI AGCT 8 cut(s) 33, 75, 115, 295, 338, 388, 497, 1126
Alw21I GWGCWC 2 cut(s) 340, 1080
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 4 cut(s) 211, 432, 445, 1002
AoxI GGCC 2 cut(s) 342, 807
ApeKI GCWGC 4 cut(s) 33, 104, 250, 253
ApoI RAATTY 5 cut(s) 408, 616, 756, 861, 976
AsiGI ACCGGT 1 cut(s) 698
AsuHPI GGTGA 1 cut(s) 857
BalI TGGCCA 1 cut(s) 344
BamHI GGATCC 1 cut(s) 437
BanII GRGCYC 1 cut(s) 340
Bbv12I GWGCWC 2 cut(s) 340, 1080
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 4 cut(s) 20, 91, 237, 240
BccI CCATC 3 cut(s) 365, 625, 797
BciT130I CCWGG 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 1 cut(s) 914
BfmI CTRYAG 2 cut(s) 254, 514
BfuAI ACCTGC 1 cut(s) 1017
BisI GCNGC 4 cut(s) 34, 105, 251, 254
BlsI GCNGC 4 cut(s) 35, 106, 252, 255
Bme1390I CCNGG 1 cut(s) 246
BmiI GGNNCC 2 cut(s) 439, 504
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 2 cut(s) 695, 814
Bpu10I CCTNAGC 1 cut(s) 498
BsaJI CCNNGG 4 cut(s) 244, 245, 507, 1027
BsaWI WCCGGW 1 cut(s) 698
Bsc4I CCNNNNNNNGG 1 cut(s) 874
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 1 cut(s) 108
Bse3DI GCAATG 2 cut(s) 457, 578
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 4 cut(s) 244, 245, 507, 1027
BseGI GGATG 2 cut(s) 376, 1107
BseLI CCNNNNNNNGG 1 cut(s) 874
BseMI GCAATG 2 cut(s) 457, 578
BseMII CTCAG 2 cut(s) 489, 864
BseNI ACTGG 1 cut(s) 108
BseXI GCAGC 4 cut(s) 20, 91, 237, 240
BsgI GTGCAG 2 cut(s) 34, 76
BshFI GGCC 2 cut(s) 344, 809
BshTI ACCGGT 1 cut(s) 698
BsiHKAI GWGCWC 2 cut(s) 340, 1080
BsiSI CCGG 1 cut(s) 699
BslFI GGGAC 1 cut(s) 962
BslI CCNNNNNNNGG 1 cut(s) 874
BsmAI GTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 962
BsmI GAATGC 2 cut(s) 471, 852
BsnI GGCC 2 cut(s) 344, 809
Bsp1286I GDGCHC 2 cut(s) 340, 1080
Bsp1407I TGTACA 2 cut(s) 677, 1104
Bsp143I GATC 5 cut(s) 136, 203, 437, 709, 994
Bsp19I CCATGG 1 cut(s) 507
BspANI GGCC 2 cut(s) 344, 809
BspCNI CTCAG 2 cut(s) 490, 865
BspLI GGNNCC 2 cut(s) 439, 504
BspMAI CTGCAG 1 cut(s) 258
BspMI ACCTGC 1 cut(s) 1017
BspPI GGATC 4 cut(s) 211, 432, 445, 1002
BsrDI GCAATG 2 cut(s) 457, 578
BsrFI RCCGGY 1 cut(s) 698
BsrGI TGTACA 2 cut(s) 677, 1104
BsrI ACTGG 1 cut(s) 108
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 4 cut(s) 244, 245, 507, 1027
BssMI GATC 5 cut(s) 136, 203, 437, 709, 994
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 1082
Bst6I CTCTTC 1 cut(s) 950
BstAUI TGTACA 2 cut(s) 677, 1104
BstC8I GCNNGC 2 cut(s) 77, 469
BstDEI CTNAG 3 cut(s) 326, 498, 873
BstDSI CCRYGG 2 cut(s) 507, 1027
BstF5I GGATG 2 cut(s) 376, 1107
BstKTI GATC 5 cut(s) 139, 206, 440, 712, 997
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 5 cut(s) 136, 203, 437, 709, 994
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 1 cut(s) 648
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 2 cut(s) 254, 514
BstV1I GCAGC 4 cut(s) 20, 91, 237, 240
BstX2I RGATCY 3 cut(s) 203, 437, 994
BstYI RGATCY 3 cut(s) 203, 437, 994
BsuRI GGCC 2 cut(s) 344, 809
BtgI CCRYGG 2 cut(s) 507, 1027
BtsCI GGATG 2 cut(s) 376, 1107
BtsIMutI CAGTG 2 cut(s) 115, 870
BveI ACCTGC 1 cut(s) 1017
Cac8I GCNNGC 2 cut(s) 77, 469
Cfr10I RCCGGY 1 cut(s) 698
Csp6I GTAC 4 cut(s) 519, 678, 1006, 1105
CspAI ACCGGT 1 cut(s) 698
CviAII CATG 5 cut(s) 487, 508, 645, 952, 1051
CviQI GTAC 4 cut(s) 519, 678, 1006, 1105
DdeI CTNAG 3 cut(s) 326, 498, 873
DpnI GATC 5 cut(s) 138, 205, 439, 711, 996
DpnII GATC 5 cut(s) 136, 203, 437, 709, 994
DraI TTTAAA 1 cut(s) 1122
EaeI YGGCCR 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 950
EarI CTCTTC 1 cut(s) 950
Ecl136II GAGCTC 1 cut(s) 338
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 340
Eco53kI GAGCTC 1 cut(s) 338
Eco57I CTGAAG 2 cut(s) 55, 236
EcoICRI GAGCTC 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 5 cut(s) 490, 511, 648, 955, 1054
FaqI GGGAC 1 cut(s) 962
FatI CATG 5 cut(s) 486, 507, 644, 951, 1050
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 4 cut(s) 34, 105, 251, 254
FokI GGATG 2 cut(s) 383, 1114
FriOI GRGCYC 1 cut(s) 340
Fsp4HI GCNGC 4 cut(s) 34, 105, 251, 254
FspBI CTAG 1 cut(s) 914
GluI GCNGC 4 cut(s) 34, 105, 251, 254
HaeIII GGCC 2 cut(s) 344, 809
HapII CCGG 1 cut(s) 699
Hin1II CATG 5 cut(s) 490, 511, 648, 955, 1054
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 5 cut(s) 263, 460, 662, 1047, 1135
HpaII CCGG 1 cut(s) 699
HphI GGTGA 1 cut(s) 857
Hpy166II GTNNAC 2 cut(s) 287, 661
Hpy188I TCNGA 6 cut(s) 159, 216, 385, 625, 709, 1162
Hpy188III TCNNGA 4 cut(s) 441, 535, 566, 731
Hpy8I GTNNAC 2 cut(s) 287, 661
HpyAV CCTTC 3 cut(s) 275, 1066, 1147
HpyCH4III ACNGT 1 cut(s) 1082
HpyCH4V TGCA 9 cut(s) 15, 57, 79, 235, 256, 471, 686, 804, 850
HpyF3I CTNAG 3 cut(s) 326, 498, 873
Hsp92II CATG 5 cut(s) 490, 511, 648, 955, 1054
Kzo9I GATC 5 cut(s) 136, 203, 437, 709, 994
LmnI GCTCC 2 cut(s) 508, 1075
Lsp1109I GCAGC 4 cut(s) 20, 91, 237, 240
LweI GCATC 2 cut(s) 695, 814
MaeI CTAG 1 cut(s) 914
MaeIII GTNAC 2 cut(s) 528, 1034
MalI GATC 5 cut(s) 138, 205, 439, 711, 996
MboI GATC 5 cut(s) 136, 203, 437, 709, 994
MboII GAAGA 4 cut(s) 181, 777, 900, 967
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 3 cut(s) 203, 437, 994
MhlI GDGCHC 2 cut(s) 340, 1080
MlsI TGGCCA 1 cut(s) 344
MluNI TGGCCA 1 cut(s) 344
MlyI GAGTC 2 cut(s) 656, 1056
MnlI CCTC 8 cut(s) 281, 428, 493, 649, 745, 868, 951, 1048
Mox20I TGGCCA 1 cut(s) 344
MscI TGGCCA 1 cut(s) 344
MseI TTAA 7 cut(s) 8, 50, 90, 431, 585, 1062, 1121
MslI CAYNNNNRTG 4 cut(s) 10, 52, 480, 940
Msp20I TGGCCA 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 1 cut(s) 699
MspR9I CCNGG 1 cut(s) 246
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 2 cut(s) 471, 852
MvaI CCWGG 1 cut(s) 246
NcoI CCATGG 1 cut(s) 507
NdeII GATC 5 cut(s) 136, 203, 437, 709, 994
NlaIII CATG 5 cut(s) 490, 511, 648, 955, 1054
NlaIV GGNNCC 2 cut(s) 439, 504
NmuCI GTSAC 1 cut(s) 528
NspI RCATGY 1 cut(s) 648
PasI CCCWGGG 1 cut(s) 245
PciI ACATGT 1 cut(s) 644
PctI GAATGC 2 cut(s) 471, 852
PfeI GAWTC 3 cut(s) 263, 460, 1135
PinAI ACCGGT 1 cut(s) 698
PkrI GCNGC 4 cut(s) 35, 106, 252, 255
PleI GAGTC 2 cut(s) 656, 1055
PpsI GAGTC 2 cut(s) 656, 1055
PscI ACATGT 1 cut(s) 644
PsiI TTATAA 1 cut(s) 353
Psp124BI GAGCTC 1 cut(s) 340
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 439, 504
PstI CTGCAG 1 cut(s) 258
PsuI RGATCY 3 cut(s) 203, 437, 994
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 4 cut(s) 520, 679, 1007, 1106
RsaNI GTAC 4 cut(s) 519, 678, 1006, 1105
RseI CAYNNNNRTG 4 cut(s) 10, 52, 480, 940
SacI GAGCTC 1 cut(s) 340
SaqAI TTAA 7 cut(s) 8, 50, 90, 431, 585, 1062, 1121
SatI GCNGC 4 cut(s) 34, 105, 251, 254
Sau3AI GATC 5 cut(s) 136, 203, 437, 709, 994
SchI GAGTC 2 cut(s) 656, 1056
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 2 cut(s) 340, 1080
SfaNI GCATC 2 cut(s) 695, 814
SfcI CTRYAG 2 cut(s) 254, 514
SmiMI CAYNNNNRTG 4 cut(s) 10, 52, 480, 940
SspMI CTAG 1 cut(s) 914
SstI GAGCTC 1 cut(s) 340
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 1 cut(s) 1082
TaqI TCGA 1 cut(s) 641
TatI WGTACW 2 cut(s) 677, 1104
TfiI GAWTC 3 cut(s) 263, 460, 1135
Tru1I TTAA 7 cut(s) 8, 50, 90, 431, 585, 1062, 1121
Tru9I TTAA 7 cut(s) 8, 50, 90, 431, 585, 1062, 1121
TscAI CASTG 2 cut(s) 115, 877
TseFI GTSAC 1 cut(s) 528
TseI GCWGC 4 cut(s) 33, 104, 250, 253
Tsp45I GTSAC 1 cut(s) 528
TspDTI ATGAA 6 cut(s) 323, 417, 464, 968, 1085, 1118
TspGWI ACGGA 2 cut(s) 914, 1016
TspRI CASTG 2 cut(s) 115, 877
XapI RAATTY 5 cut(s) 408, 616, 756, 861, 976
XceI RCATGY 1 cut(s) 648
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.