AT1G01960

C-terminal region of Mon2 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007735
Physical Location & Seq
Reverse (-)
330588 .. 337912
7325 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01960.1

Sequence Viewer

Length: 5253 bp
ATGGCTTCTACGGAAGTCGATTCCCGCTTAGGTCGAGTTGTTATCCCTGCTTTGGACAAGGTCATTAAGAACGCCTCCTGGCGTAAGCACTCCAAGCTCGCCCACGAGTGCAAATCCGTCATCGAACGCCTTAGATCCCCTGAAAATTCCTCTCCTGTTGCCGACTCTGAATCTGGATCCTCCATTCCTGGACCACTTCACGACGGAGGAGCCGCCGAATACTCTCTCGCCGAGTCCGAGATCATCCTCAGCCCTCTCATCAACGCCTCCTCCACCGGCGTTCTCAAGATTGTCGATCCCGCCGTTGATTGCATCCAGAAGCTTATTGCTCATGGCTACGTTCGCGGTGAGGCTGATCCCACCGGCGGACCTGAGGCGCTACTCCTTTCCAAGCTCATTGAAACCATCTGCAAATGTCACGAGCTAGACGACGAAGGGCTCGAGTTGCTCGTGCTGAAGACGCTCTTGACTGCGGTAACTTCAATTTCCCTGAGGATCCATGGAGATTCTTTGCTACAGATCGTCAGGACTTGTTATGGTATCTACTTGGGAAGCAGAAACGTGGTTAATCAGGCGACTGCTAAAGCCTCGCTCGTGCAGATGTCTGTTATCGTGTTCAGAAGAATGGAGGCAGATTCATCCACCGTCCCCATTCAGCCCATCGTCGTGGCCGAGCTGATGGAGCCCATGGACAAATCCGAATCAGACCCATCTACGACGCAGTCTGTTCAAGGTTTCATTACCAAGATTATGCAGGACATTGACGGAGTCTTCAATTCAGCTAACGCCAAGGGCACATTCGGCGGCCATGACGGGGCCTTCGAGACTTCATTGCCTGGCACTGCCAATCCTACTGATTTGTTGGATTCTACTGATAAGGATATGTTGGATGCCAAGTACTGGGAGATCAGTATGTATAAGTCGGCCCTCGAGGGAAGAAAAGGAGAGCTGGCCGATGGAGAAGTGGAGAAAGACGATGATTCTGAAGTTCAGATTGGGAATAAGCTGAGAAGAGACGCCTTCTTGGTTTTCAGGGCACTTTGCAAGCTGTCCATGAAGACGCCTCCCAAGGAAGATCCAGAGTTGATGAGAGGGAAGATTGTGGCTCTCGAGTTATTGAAGATTCTGCTTGAGAATGCCGGTGCTGTCTTTAGAACCAGCGATAGGTTCTTAGGCGCCATCAAGCAGTATCTGTGTCTCTCCTTGCTGAAGAACAGTGCTTCAAATCTTATGATCATTTTCCAGCTTTCCTGCTCGATTTTACTTAGCTTGGTTTCGAGGTTTCGTGCTGGGCTGAAGGCAGAGATTGGGGTGTTCTTCCCTATGATTGTTCTAAGAGTATTAGAGAATGTTGCTCAACCTGATTTCCAACAGAAGATGATAGTGCTTCGGTTCTTGGACAAGCTCTGCGTTGATTCACAGATTTTGGTAGATATCTTCATCAACTATGATTGCGATGTCAACTCATCCAATATATTTGAGAGGATGGTCAATGGTCTCCTTAAGACTGCTCAAGGAGTGCCTCCTGGTACAGTTACTACCTTGTTGCCACCTCAGGAAGCAGCCATGAAGCTTGAAGCCATGAAATGCTTAGTTGCTGTTTTAAGGTCTATGGGAGACTGGGTGAATAAGCAGTTGCGTCTTCCAGATCCTTATTCGGCAAAAATGCTGGAAATTGTTGATAGAAATCTTGAAGAAGGAAGTCATCCAGTAGAAAATGGTAAGGGGGATGGAGGCCATGGTGGTTTTGAAAGATCAGACTCTCAGTCTGAATTGTCCAGTGGGAATTCTGATGCCTTAGCAATAGAGCAGCGCCGAGCTTACAAGCTTGAACTTCAGGAAGGTATTTCTATTTTCAACCAGAAACCTAAGAAAGGTATTGAATTTTTAATCAAGGCAAACAAGGTGGGGGACTCGCCAGAGGAAATAGCAGCTTTTCTCAAAGATGCATCAGGGTTGAACAAGACTTTGATAGGGGATTATCTGGGGGAAAGAGAAGACTTATCTCTGAAAGTGATGCATGCATATGTGGATTCTTTCGAATTTCAAGGCATGGAATTTGATGAGGCAATTCGAGCCTTTCTCCGGGGCTTTAGGCTGCCAGGAGAAGCACAAAAAATCGATCGTATAATGGAAAAATTTGCAGAGCGATTCTGTAAATGCAACCCAAAGGATTTTTCTAGCGCTGATACGGCATATGTCCTCGCTTACTCAGTCATTTTGCTCAACACAGATGCACATAACCCTATGGTGAAGAGCAAGATGACAGCTGATGGTTTTATTAGAAACAATCGTGGTATAGATGATGGGAAAGATTTGCCTGAGGAATATCTACGTGCATTATATGAAAGGATATCTAGAAATGAGATAAAGATGAAGGATGATGGTTTGGGTCCGCAGCAGAAGCAGCCTACAAACTCAAGTAGATTACTTGGCTTAGATACTATCTTAAATATTGTTGTTCCTAGGCGTGGTGACGATATGAACATGGAGACCAGTGATGATCTTATCAGGCATATGCAAGAGAGATTTAAAGAAAAGGCTCGCAAATCTGAGTCAGTTTATTATGCAGCCTCGGATGTGATTATACTCAGATTTATGGTTGAGGTTTGTTGGGCTCCTATGTTGGCAGCTTTCAGTGTTCCACTTGACCAGAGTGATGATGCAGTAATAACAACATTATGTCTGGAGGGTTTCCATCATGCCATCCATGTCACTTCTGTAATGTCCTTAAAAACGCACAGAGATGCCTTTGTGACTTCACTAGCAAAATTTACTTCCCTCCACTCTCCTGCTGATATCAAGCAAAAAAACATTGAAGCAATAAAGGCAATAGTAAAACTGGCGGAGGAAGAAGGAAACTATCTACAAGATGCTTGGGAGCATATACTGACTTGTGTTTCACGGTTTGAGCATCTTCATCTCTTGGGGGAAGGAGCGCCTCCTGATGCCACTTTCTTTGCATTTCCTCAAACGGAGTCAGGAAACTCTCCACTGGCTAAGCCAAATTCCGTACCTGCAATTAAAGAAAGAGCACCTGGAAAACTTCAGTATGCTGCATCTGCTATGATAAGGGGTTCCTACGATGGTTCTGGTGTTGCTGGAAAGGCTTCTAATACAGTGACATCTGAACAGATGAACAACTTGATATCTAATCTAAACTTGCTAGAACAAGTTGGTGACATGAGTCGGATATTTACTCGTAGTCAAAGATTGAATAGTGAAGCCATAATTGACTTTGTTAAAGCCCTCTGCAAAGTTTCCATGGATGAGTTACGATCTCCATCTGATCCACGGGTCTTCAGCCTAACAAAGATTGTGGAAATAGCGCACTATAACATGAACCGCATAAGACTTGTCTGGTCGAGCATCTGGCATGTGCTCTCTGATTTCTTTGTGACTATTGGCTGTTCTGATAACCTCTCCATTGCAATTTTTGCAATGGATTCTTTGCGCCAGCTGTCTATGAAGTTCTTAGAGCGGGAAGAGTTGGCGAATTATAACTTCCAAAATGAATTTATGAAGCCTTTTGTTGTTGTCATGCGCAAAAGTGGTGCTGTTGAGATCAGGGAATTGATTATACGATGTGTCTCACAGATGGTTTTATCCCGTGTTGACAATGTCAAGTCGGGATGGAAGAGTATGTTCATGATTTTCACGACAGCAGCACATGATGCACATAAAAATATTGTGTTCCTGTCTTTTGAAATGGTTGAGAAGATTATCCGAGACTACTTCCCACACATCACGGAGACAGAGACAACCACCTTCACAGACTGTGTGAATTGCCTTGTTGCGTTCACCAATTGTAAATTTGAGAAAGATATTAGTCTCCAAGCCATTGCTTTCCTTCAGTATTGCGCAAGAAAACTTGCTGAAGGCTATGTTGGTTCGTCACTGAGGAGGAACCCACCATTATCTCCTCAGGGTGGAAAAATTGGAAAACAAGATAGTGGGAAGTTTCTAGAAAGTGATGAACATTTGTATTCTTGGTTTCCACTGTTAGCCGGTTTATCTGAACTTAGTTTCGACCCAAGAGCAGAAATTAGGAAGGTTGCATTGAAAGTCCTGTTTGATACTCTGAGGAACCATGGTGACCATTTCAGTCTGGCGTTATGGGAACGGGTTTTTGAGTCTGTTTTATTTCGAATATTCGACTATGTGCGTCAGGACGTTGACCCATCTGAGGATGATTCAACAGATCAGAGAGGTTACAATGGTGAAGTCGACCAAGAGTCTTGGCTTTATGAGACATGCTCGTTAGCTCTACAGCTGGTTGTAGACCTCTTTGTAAATTTTTACAAGACGGTTAACCCTCTCCTTAAGAAGGTACTGATGTTATTTGTAAGCTTGATAAAACGTCCTCACCAAAGTCTTGCTGGTGCGGGTATTGCTGCGCTTGTCCGTCTGATGAGAGATGTCGGTCATCAATTCTCTAACGAACAATGGCTCGAGGTTGTGTCATGTATCAAAGAAGCAGCAGATGCTACATCCCCTGACTTCTCATACGTTACCAGTGAAGATTTGATGGAGGATGTAAGCAATGAGGACGAAACAAACGATAATTCCAACGACGCACTGAGAAGAAGGAACAGGCAGCTTCATGCTGTCGTCACAGATGCCAAATCTAAAGCTTCAATTCAGATTTTTGTGATTCAGGCGGTAACAGATATCTACGACATGTACCGTATGTCTCTCACAGCCAATCACATGTTGATGCTCTTCGACGCTATGCACGGCATTGGATCCAACGCTCACAAAATCAACGCTGATCTACTCTTACGTTCCAAGCTCCAGGAGCTCGGGTCGTCCCTTGAATCCCAAGAAGCTCCTCTGCTGCGCCTGGAGAACGAATCTTTCCAAACATGTATGACCTTCTTAGACAACCTCATCTCAGATCAGCCTGTGGGTTACAACGAGGCTGAAATAGAATCCCACCTCATAAGCCTCTGCAGAGAGGTGTTAGAGTTCTACATAAATATTTCATGTTCAAAGGAGCAGTCTTCAAGGTGGGCGGTTCCCTCGGGGTCAGGAAAAAAGAAAGAGCTAACAGCGCGGGCTCCGCTAGTGGTGGCTGCAATTCAGACATTGGGAAACATGGGAGAGTCACTGTTCAAGAAGAACTTGCCGGAGTTGTTTCCATTGATAGCGACGTTGATAAGCTGTGAACATGGGTCAGGAGAGGTACAAGTAGCTCTTAGTGATATGCTTCAGACATCAATGGGACCTGTTCTCCTCCGCTCTTGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1750

Amino Acids

194.94

Weight (kDa)

5.43

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 7 - 212 1.6e-42 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 337 - 492 1.6e-47 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 609 - 790 1.2e-76 Sec7 domain
Sec7-like_HDS PF09324 1157 - 1239 9.3e-30 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1331 - 1402 5.1e-07 C-terminal region of Mon2 protein
BIG2_C PF20252 1547 - 1738 6.7e-43 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3501
AbsI CCTCGAGG 1 cut(s) 929
Acc16I TGCGCA 2 cut(s) 3545, 3862
Acc36I ACCTGC 1 cut(s) 3025
AccB1I GGYRCC 1 cut(s) 1175
AccB7I CCANNNNNTGG 1 cut(s) 900
AccBSI CCGCTC 2 cut(s) 3481, 5241
AccI GTMKAC 2 cut(s) 4227, 4281
AccII CGCG 2 cut(s) 345, 5056
AcoI YGGCCR 3 cut(s) 669, 805, 951
AcyI GRCGYC 3 cut(s) 1017, 1061, 1176
AfaI GTAC 6 cut(s) 899, 1531, 3015, 4332, 4685, 5187
AfeI AGCGCT 1 cut(s) 2185
AflII CTTAAG 2 cut(s) 1502, 4322
AflIII ACRYGT 3 cut(s) 4680, 4710, 4865
AhdI GACNNNNNGTC 2 cut(s) 1765, 4234
AjnI CCWGG 8 cut(s) 77, 187, 835, 1525, 2101, 3037, 4794, 4842
AjuI GAANNNNNNNTTGG 8 cut(s) 782, 814, 978, 1010, 2161, 2193, 3870, 3902
Alw21I GWGCWC 3 cut(s) 3037, 3384, 4803
AlwNI CAGNNNCTG 1 cut(s) 1192
Ama87I CYCGRG 6 cut(s) 440, 929, 1109, 4451, 4802, 5023
Aor51HI AGCGCT 1 cut(s) 2185
AoxI GGCC 6 cut(s) 669, 805, 816, 924, 951, 1735
ArsI GACNNNNNNTTYG 4 cut(s) 803, 835, 4985, 5017
Asp700I GAANNNNTTC 1 cut(s) 3109
AspA2I CCTAGG 1 cut(s) 2465
AspS9I GGNCC 6 cut(s) 191, 368, 816, 925, 2393, 5225
AsuC2I CCSGG 1 cut(s) 2087
AsuHPI GGTGA 9 cut(s) 359, 1636, 2263, 2486, 3191, 3793, 4106, 4232, 4358
AsuII TTCGAA 2 cut(s) 2040, 4147
AvaI CYCGRG 6 cut(s) 440, 929, 1109, 4451, 4802, 5023
AvaII GGWCC 4 cut(s) 191, 368, 2393, 5225
AvrII CCTAGG 1 cut(s) 2465
AxyI CCTNAGG 5 cut(s) 372, 491, 1554, 2322, 3924
BaeGI GKGCMC 2 cut(s) 797, 1039
BaeI ACNNNNGTAYC 2 cut(s) 4322, 4355
BamHI GGATCC 3 cut(s) 176, 495, 4745
BanI GGYRCC 1 cut(s) 1175
BanII GRGCYC 5 cut(s) 441, 687, 2620, 4803, 5062
BauI CACGAG 4 cut(s) 104, 419, 449, 593
BbsI GAAGAC 7 cut(s) 464, 763, 1064, 1634, 2004, 3292, 4995
Bbv12I GWGCWC 3 cut(s) 3037, 3384, 4803
BbvCI CCTCAGC 1 cut(s) 248
BceAI ACGGC 3 cut(s) 287, 2208, 4753
BciT130I CCWGG 8 cut(s) 79, 189, 837, 1527, 2103, 3039, 4796, 4844
BclI TGATCA 1 cut(s) 1233
BcnI CCSGG 1 cut(s) 2087
BfaI CTAG 8 cut(s) 425, 2181, 2358, 2466, 2765, 3167, 3965, 5066
BfmI CTRYAG 3 cut(s) 515, 4268, 4951
BfoI RGCGCY 5 cut(s) 380, 1179, 1816, 2187, 2942
BfrI CTTAAG 2 cut(s) 1502, 4322
BfuAI ACCTGC 1 cut(s) 3025
BlnI CCTAGG 1 cut(s) 2465
BlpI GCTNAGC 1 cut(s) 3000
BmcAI AGTACT 1 cut(s) 899
Bme1390I CCNGG 9 cut(s) 79, 189, 837, 1527, 2087, 2103, 3039, 4796, 4844
Bme18I GGWCC 4 cut(s) 191, 368, 2393, 5225
BmeRI GACNNNNNGTC 2 cut(s) 1765, 4234
BmeT110I CYCGRG 6 cut(s) 440, 929, 1109, 4451, 4802, 5023
BmgT120I GGNCC 6 cut(s) 191, 368, 816, 925, 2393, 5225
BmrFI CCNGG 9 cut(s) 79, 189, 837, 1527, 2087, 2103, 3039, 4796, 4844
BmrI ACTGGG 2 cut(s) 910, 1630
BmuI ACTGGG 2 cut(s) 910, 1630
BoxI GACNNNNGTC 1 cut(s) 3186
BpiI GAAGAC 7 cut(s) 464, 763, 1064, 1634, 2004, 3292, 4995
BplI GAGNNNNNCTC 4 cut(s) 2067, 2099, 4241, 4273
BpmI CTGGAG 3 cut(s) 2708, 4778, 4865
Bpu10I CCTNAGC 3 cut(s) 28, 248, 1798
Bpu1102I GCTNAGC 1 cut(s) 3000
Bpu14I TTCGAA 2 cut(s) 2040, 4147
BpuEI CTTGAG 4 cut(s) 269, 1151, 1497, 2404
BpuMI CCSGG 1 cut(s) 2087
Bsa29I ATCGAT 1 cut(s) 2121
BsaAI YACGTR 1 cut(s) 2336
BsaBI GATNNNNATC 2 cut(s) 1686, 3283
BsaHI GRCGYC 3 cut(s) 1017, 1061, 1176
BsaI GGTCTC 2 cut(s) 1502, 2486
BsaXI ACNNNNNCTCC 8 cut(s) 254, 284, 1608, 1638, 4790, 4820, 5217, 5247
Bse118I RCCGGY 4 cut(s) 275, 362, 1139, 4007
Bse1I ACTGG 8 cut(s) 905, 1625, 1709, 1779, 2496, 2847, 3000, 4515
Bse21I CCTNAGG 5 cut(s) 372, 491, 1554, 2322, 3924
Bse3DI GCAATG 5 cut(s) 830, 3426, 3447, 3840, 4549
Bse8I GATNNNNATC 2 cut(s) 1686, 3283
BseBI CCWGG 8 cut(s) 79, 189, 837, 1527, 2103, 3039, 4796, 4844
BseCI ATCGAT 1 cut(s) 2121
BseJI GATNNNNATC 2 cut(s) 1686, 3283
BseMI GCAATG 5 cut(s) 830, 3426, 3447, 3840, 4549
BseNI ACTGG 8 cut(s) 905, 1625, 1709, 1779, 2496, 2847, 3000, 4515
BseRI GAGGAG 6 cut(s) 222, 259, 3912, 3916, 4821, 5225
BseSI GKGCMC 2 cut(s) 797, 1039
BseYI CCCAGC 1 cut(s) 1289
BsgI GTGCAG 1 cut(s) 617
Bsh1236I CGCG 2 cut(s) 345, 5056
Bsh1285I CGRYCG 1 cut(s) 2125
BshFI GGCC 6 cut(s) 671, 807, 818, 926, 953, 1737
BshNI GGYRCC 1 cut(s) 1175
BshVI ATCGAT 1 cut(s) 2121
BsiEI CGRYCG 1 cut(s) 2125
BsiHKAI GWGCWC 3 cut(s) 3037, 3384, 4803
BsiHKCI CYCGRG 6 cut(s) 440, 929, 1109, 4451, 4802, 5023
BsiSI CCGG 6 cut(s) 276, 363, 1140, 2086, 4008, 5129
BslFI GGGAC 4 cut(s) 632, 1925, 4795, 5238
BsmBI CGTCTC 1 cut(s) 1008
BsmFI GGGAC 4 cut(s) 632, 1925, 4795, 5238
BsmI GAATGC 1 cut(s) 1141
BsnI GGCC 6 cut(s) 671, 807, 818, 926, 953, 1737
Bso31I GGTCTC 2 cut(s) 1502, 2486
BsoBI CYCGRG 6 cut(s) 440, 929, 1109, 4451, 4802, 5023
Bsp119I TTCGAA 2 cut(s) 2040, 4147
Bsp1286I GDGCHC 9 cut(s) 441, 687, 797, 1039, 2620, 3037, 3384, 4803, 5062
Bsp1720I GCTNAGC 1 cut(s) 3000
Bsp19I CCATGG 5 cut(s) 499, 687, 1738, 3264, 4090
BspANI GGCC 6 cut(s) 671, 807, 818, 926, 953, 1737
BspDI ATCGAT 1 cut(s) 2121
BspFNI CGCG 2 cut(s) 345, 5056
BspHI TCATGA 1 cut(s) 3648
BspMAI CTGCAG 1 cut(s) 4955
BspMI ACCTGC 1 cut(s) 3025
BspQI GCTCTTC 2 cut(s) 2249, 4727
BspT104I TTCGAA 2 cut(s) 2040, 4147
BspT107I GGYRCC 1 cut(s) 1175
BspTI CTTAAG 2 cut(s) 1502, 4322
BspTNI GGTCTC 2 cut(s) 1502, 2486
BsrBI CCGCTC 2 cut(s) 3481, 5241
BsrDI GCAATG 5 cut(s) 830, 3426, 3447, 3840, 4549
BsrFI RCCGGY 4 cut(s) 275, 362, 1139, 4007
BsrI ACTGG 8 cut(s) 905, 1625, 1709, 1779, 2496, 2847, 3000, 4515
BssAI RCCGGY 4 cut(s) 275, 362, 1139, 4007
BssNI GRCGYC 3 cut(s) 1017, 1061, 1176
BssSI CACGAG 4 cut(s) 104, 419, 449, 593
BssT1I CCWWGG 8 cut(s) 499, 687, 789, 1068, 1738, 2465, 3264, 4090
Bst2BI CACGAG 4 cut(s) 104, 419, 449, 593
Bst2UI CCWGG 8 cut(s) 79, 189, 837, 1527, 2103, 3039, 4796, 4844
Bst6I CTCTTC 5 cut(s) 1006, 2249, 3480, 3632, 4727
BstACI GRCGYC 3 cut(s) 1017, 1061, 1176
BstAFI CTTAAG 2 cut(s) 1502, 4322
BstAPI GCANNNNNTGC 3 cut(s) 3437, 3674, 4484
BstBAI YACGTR 1 cut(s) 2336
BstBI TTCGAA 2 cut(s) 2040, 4147
BstC8I GCNNGC 7 cut(s) 99, 951, 1046, 2022, 2545, 3458, 5058
BstDSI CCRYGG 6 cut(s) 499, 687, 1738, 3264, 3293, 4090
BstEII GGTNACC 1 cut(s) 4094
BstENI CCTNNNNNAGG 2 cut(s) 1872, 4325
BstFNI CGCG 2 cut(s) 345, 5056
BstH2I RGCGCY 5 cut(s) 380, 1179, 1816, 2187, 2942
BstMCI CGRYCG 1 cut(s) 2125
BstNI CCWGG 8 cut(s) 79, 189, 837, 1527, 2103, 3039, 4796, 4844
BstNSI RCATGY 6 cut(s) 2024, 3380, 4257, 4684, 4714, 4869
BstPAI GACNNNNGTC 1 cut(s) 3186
BstPI GGTNACC 1 cut(s) 4094
BstSCI CCNGG 9 cut(s) 77, 187, 835, 1525, 2085, 2101, 3037, 4794, 4842
BstSFI CTRYAG 3 cut(s) 515, 4268, 4951
BstSLI GKGCMC 2 cut(s) 797, 1039
BstUI CGCG 2 cut(s) 345, 5056
BstV2I GAAGAC 7 cut(s) 464, 763, 1064, 1634, 2004, 3292, 4995
BstX2I RGATCY 6 cut(s) 134, 176, 495, 1075, 1648, 4745
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 6 cut(s) 134, 176, 495, 1075, 1648, 4745
Bsu15I ATCGAT 1 cut(s) 2121
Bsu36I CCTNAGG 5 cut(s) 372, 491, 1554, 2322, 3924
BsuRI GGCC 6 cut(s) 671, 807, 818, 926, 953, 1737
BsuTUI ATCGAT 1 cut(s) 2121
BtgI CCRYGG 6 cut(s) 499, 687, 1738, 3264, 3293, 4090
BtgZI GCGATG 1 cut(s) 1470
BtsI GCAGTG 1 cut(s) 840
BveI ACCTGC 1 cut(s) 3025
Cac8I GCNNGC 7 cut(s) 99, 951, 1046, 2022, 2545, 3458, 5058
CaiI CAGNNNCTG 1 cut(s) 1192
CciI TCATGA 1 cut(s) 3648
Cfr10I RCCGGY 4 cut(s) 275, 362, 1139, 4007
Cfr13I GGNCC 6 cut(s) 191, 368, 816, 925, 2393, 5225
ClaI ATCGAT 1 cut(s) 2121
CseI GACGC 8 cut(s) 469, 727, 1025, 1069, 1628, 4154, 4583, 4736
Csp6I GTAC 6 cut(s) 898, 1530, 3014, 4331, 4684, 5186
CspCI CAANNNNNGTGG 6 cut(s) 1887, 1922, 2941, 2976, 3300, 3335
CviQI GTAC 6 cut(s) 898, 1530, 3014, 4331, 4684, 5186
DinI GGCGCC 1 cut(s) 1177
DraI TTTAAA 1 cut(s) 2533
DriI GACNNNNNGTC 2 cut(s) 1765, 4234
EaeI YGGCCR 3 cut(s) 669, 805, 951
Eam1104I CTCTTC 5 cut(s) 1006, 2249, 3480, 3632, 4727
Eam1105I GACNNNNNGTC 2 cut(s) 1765, 4234
EarI CTCTTC 5 cut(s) 1006, 2249, 3480, 3632, 4727
EciI GGCGGA 2 cut(s) 381, 2861
Ecl136II GAGCTC 1 cut(s) 4801
Eco130I CCWWGG 8 cut(s) 499, 687, 789, 1068, 1738, 2465, 3264, 4090
Eco24I GRGCYC 5 cut(s) 441, 687, 2620, 4803, 5062
Eco31I GGTCTC 2 cut(s) 1502, 2486
Eco32I GATATC 5 cut(s) 1435, 2355, 2800, 3150, 4672
Eco47I GGWCC 4 cut(s) 191, 368, 2393, 5225
Eco47III AGCGCT 1 cut(s) 2185
Eco53kI GAGCTC 1 cut(s) 4801
Eco81I CCTNAGG 5 cut(s) 372, 491, 1554, 2322, 3924
Eco88I CYCGRG 6 cut(s) 440, 929, 1109, 4451, 4802, 5023
Eco91I GGTNACC 1 cut(s) 4094
EcoICRI GAGCTC 1 cut(s) 4801
EcoNI CCTNNNNNAGG 2 cut(s) 1872, 4325
EcoO109I RGGNCCY 2 cut(s) 816, 5225
EcoO65I GGTNACC 1 cut(s) 4094
EcoRI GAATTC 1 cut(s) 1786
EcoRII CCWGG 8 cut(s) 77, 187, 835, 1525, 2101, 3037, 4794, 4842
EcoRV GATATC 5 cut(s) 1435, 2355, 2800, 3150, 4672
EcoT14I CCWWGG 8 cut(s) 499, 687, 789, 1068, 1738, 2465, 3264, 4090
EcoT22I ATGCAT 3 cut(s) 1951, 2022, 2026
EcoT38I GRGCYC 5 cut(s) 441, 687, 2620, 4803, 5062
EgeI GGCGCC 1 cut(s) 1177
EheI GGCGCC 1 cut(s) 1177
ErhI CCWWGG 8 cut(s) 499, 687, 789, 1068, 1738, 2465, 3264, 4090
Esp3I CGTCTC 1 cut(s) 1008
FalI AAGNNNNNCTT 6 cut(s) 449, 481, 5108, 5140, 5181, 5213
FaqI GGGAC 4 cut(s) 632, 1925, 4795, 5238
FauI CCCGC 5 cut(s) 32, 307, 3474, 4378, 5049
FauNDI CATATG 3 cut(s) 2026, 2197, 2517
FbaI TGATCA 1 cut(s) 1233
FblI GTMKAC 2 cut(s) 4227, 4281
FriOI GRGCYC 5 cut(s) 441, 687, 2620, 4803, 5062
FspBI CTAG 8 cut(s) 425, 2181, 2358, 2466, 2765, 3167, 3965, 5066
FspI TGCGCA 2 cut(s) 3545, 3862
GsaI CCCAGC 1 cut(s) 1293
GsuI CTGGAG 3 cut(s) 2708, 4778, 4865
HaeII RGCGCY 5 cut(s) 380, 1179, 1816, 2187, 2942
HaeIII GGCC 6 cut(s) 671, 807, 818, 926, 953, 1737
HapII CCGG 6 cut(s) 276, 363, 1140, 2086, 4008, 5129
HgaI GACGC 8 cut(s) 469, 727, 1025, 1069, 1628, 4154, 4583, 4736
Hin1I GRCGYC 3 cut(s) 1017, 1061, 1176
HincII GTYRAC 5 cut(s) 1462, 3616, 4177, 4228, 4312
HindII GTYRAC 5 cut(s) 1462, 3616, 4177, 4228, 4312
HindIII AAGCTT 5 cut(s) 320, 1571, 1826, 4348, 4632
HpaI GTTAAC 1 cut(s) 4312
HpaII CCGG 6 cut(s) 276, 363, 1140, 2086, 4008, 5129
HphI GGTGA 9 cut(s) 359, 1636, 2263, 2486, 3191, 3793, 4106, 4232, 4358
Hpy166II GTNNAC 8 cut(s) 1462, 3616, 3801, 4177, 4228, 4282, 4312, 5168
Hpy8I GTNNAC 8 cut(s) 1462, 3616, 3801, 4177, 4228, 4282, 4312, 5168
Hpy99I CGWCG 7 cut(s) 206, 434, 668, 721, 4577, 4730, 5155
HpyCH4IV ACGT 8 cut(s) 339, 561, 2335, 4173, 4360, 4509, 4783, 5153
HpySE526I ACGT 8 cut(s) 339, 561, 2335, 4173, 4360, 4509, 4783, 5153
Hsp92I GRCGYC 3 cut(s) 1017, 1061, 1176
KasI GGCGCC 1 cut(s) 1175
Ksp22I TGATCA 1 cut(s) 1233
KspAI GTTAAC 1 cut(s) 4312
LguI GCTCTTC 2 cut(s) 2249, 4727
MaeI CTAG 8 cut(s) 425, 2181, 2358, 2466, 2765, 3167, 3965, 5066
MaeII ACGT 8 cut(s) 339, 561, 2335, 4173, 4360, 4509, 4783, 5153
MbiI CCGCTC 2 cut(s) 3481, 5241
MfeI CAATTG 1 cut(s) 3805
MflI RGATCY 6 cut(s) 134, 176, 495, 1075, 1648, 4745
MhlI GDGCHC 9 cut(s) 441, 687, 797, 1039, 2620, 3037, 3384, 4803, 5062
Mly113I GGCGCC 1 cut(s) 1176
MmeI TCCRAC 6 cut(s) 843, 867, 1393, 3170, 4593, 4773
Mph1103I ATGCAT 3 cut(s) 1951, 2022, 2026
MroXI GAANNNNTTC 1 cut(s) 3109
MslI CAYNNNNRTG 4 cut(s) 665, 2025, 2745, 4715
MspA1I CMGCKG 3 cut(s) 2270, 3460, 4273
MspCI CTTAAG 2 cut(s) 1502, 4322
MspI CCGG 6 cut(s) 276, 363, 1140, 2086, 4008, 5129
MspR9I CCNGG 9 cut(s) 79, 189, 837, 1527, 2087, 2103, 3039, 4796, 4844
MunI CAATTG 1 cut(s) 3805
Mva1269I GAATGC 1 cut(s) 1141
MvaI CCWGG 8 cut(s) 79, 189, 837, 1527, 2103, 3039, 4796, 4844
MvnI CGCG 2 cut(s) 345, 5056
NarI GGCGCC 1 cut(s) 1176
NciI CCSGG 1 cut(s) 2087
NcoI CCATGG 5 cut(s) 499, 687, 1738, 3264, 4090
NdeI CATATG 3 cut(s) 2026, 2197, 2517
NmeAIII GCCGAG 3 cut(s) 256, 697, 1841
NsbI TGCGCA 2 cut(s) 3545, 3862
NsiI ATGCAT 3 cut(s) 1951, 2022, 2026
NspI RCATGY 6 cut(s) 2024, 3380, 4257, 4684, 4714, 4869
NspV TTCGAA 2 cut(s) 2040, 4147
PaeI GCATGC 1 cut(s) 2024
PaeR7I CTCGAG 4 cut(s) 440, 929, 1109, 4451
PagI TCATGA 1 cut(s) 3648
PciI ACATGT 3 cut(s) 4680, 4710, 4865
PciSI GCTCTTC 2 cut(s) 2249, 4727
PcsI WCGNNNNNNNCGW 8 cut(s) 234, 300, 426, 438, 447, 669, 819, 4557
PctI GAATGC 1 cut(s) 1141
PdmI GAANNNNTTC 1 cut(s) 3109
PflFI GACNNNGTC 4 cut(s) 59, 721, 767, 3620
PflMI CCANNNNNTGG 1 cut(s) 900
PfoI TCCNGGA 2 cut(s) 187, 4794
Ple19I CGATCG 1 cut(s) 2125
PluTI GGCGCC 1 cut(s) 1179
Ppu21I YACGTR 1 cut(s) 2336
PpuMI RGGWCCY 1 cut(s) 5225
PscI ACATGT 3 cut(s) 4680, 4710, 4865
PshAI GACNNNNGTC 1 cut(s) 3186
PsiI TTATAA 1 cut(s) 3501
Psp124BI GAGCTC 1 cut(s) 4803
Psp5II RGGWCCY 1 cut(s) 5225
Psp6I CCWGG 8 cut(s) 77, 187, 835, 1525, 2101, 3037, 4794, 4842
PspEI GGTNACC 1 cut(s) 4094
PspFI CCCAGC 1 cut(s) 1289
PspGI CCWGG 8 cut(s) 77, 187, 835, 1525, 2101, 3037, 4794, 4842
PspPI GGNCC 6 cut(s) 191, 368, 816, 925, 2393, 5225
PspPPI RGGWCCY 1 cut(s) 5225
PspXI VCTCGAGB 3 cut(s) 440, 929, 4451
PstI CTGCAG 1 cut(s) 4955
PstNI CAGNNNCTG 1 cut(s) 1192
PsuI RGATCY 6 cut(s) 134, 176, 495, 1075, 1648, 4745
PsyI GACNNNGTC 4 cut(s) 59, 721, 767, 3620
PvuI CGATCG 1 cut(s) 2125
PvuII CAGCTG 3 cut(s) 2270, 3460, 4273
RsaI GTAC 6 cut(s) 899, 1531, 3015, 4332, 4685, 5187
RsaNI GTAC 6 cut(s) 898, 1530, 3014, 4331, 4684, 5186
RseI CAYNNNNRTG 4 cut(s) 665, 2025, 2745, 4715
SacI GAGCTC 1 cut(s) 4803
SalI GTCGAC 1 cut(s) 4226
SapI GCTCTTC 2 cut(s) 2249, 4727
Sau96I GGNCC 6 cut(s) 191, 368, 816, 925, 2393, 5225
ScaI AGTACT 1 cut(s) 899
ScrFI CCNGG 9 cut(s) 79, 189, 837, 1527, 2087, 2103, 3039, 4796, 4844
SduI GDGCHC 9 cut(s) 441, 687, 797, 1039, 2620, 3037, 3384, 4803, 5062
SfcI CTRYAG 3 cut(s) 515, 4268, 4951
SfoI GGCGCC 1 cut(s) 1177
Sfr274I CTCGAG 4 cut(s) 440, 929, 1109, 4451
SfuI TTCGAA 2 cut(s) 2040, 4147
SgrAI CRCCGGYG 2 cut(s) 275, 362
SinI GGWCC 4 cut(s) 191, 368, 2393, 5225
SlaI CTCGAG 4 cut(s) 440, 929, 1109, 4451
SmiMI CAYNNNNRTG 4 cut(s) 665, 2025, 2745, 4715
SphI GCATGC 1 cut(s) 2024
SspDI GGCGCC 1 cut(s) 1175
SspI AATATT 4 cut(s) 2455, 3688, 4152, 4981
SspMI CTAG 8 cut(s) 425, 2181, 2358, 2466, 2765, 3167, 3965, 5066
SstI GAGCTC 1 cut(s) 4803
StyD4I CCNGG 9 cut(s) 77, 187, 835, 1525, 2085, 2101, 3037, 4794, 4842
StyI CCWWGG 8 cut(s) 499, 687, 789, 1068, 1738, 2465, 3264, 4090
TaiI ACGT 8 cut(s) 342, 564, 2338, 4176, 4363, 4512, 4786, 5156
TaqII GACCGA 1 cut(s) 4412
TatI WGTACW 1 cut(s) 897
TauI GCSGC 2 cut(s) 215, 807
TspGWI ACGGA 8 cut(s) 26, 106, 219, 780, 2990, 3001, 3764, 4394
Tth111I GACNNNGTC 4 cut(s) 59, 721, 767, 3620
Van91I CCANNNNNTGG 1 cut(s) 900
Vha464I CTTAAG 2 cut(s) 1502, 4322
VpaK11BI GGWCC 4 cut(s) 191, 368, 2393, 5225
XagI CCTNNNNNAGG 2 cut(s) 1872, 4325
XbaI TCTAGA 2 cut(s) 2357, 3964
XceI RCATGY 6 cut(s) 2024, 3380, 4257, 4684, 4714, 4869
XcmI CCANNNNNNNNNTGG 1 cut(s) 4376
XhoI CTCGAG 4 cut(s) 440, 929, 1109, 4451
XmaJI CCTAGG 1 cut(s) 2465
XmiI GTMKAC 2 cut(s) 4227, 4281
XmnI GAANNNNTTC 1 cut(s) 3109
XspI CTAG 8 cut(s) 425, 2181, 2358, 2466, 2765, 3167, 3965, 5066
ZrmI AGTACT 1 cut(s) 899
Zsp2I ATGCAT 3 cut(s) 1951, 2022, 2026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.