RchiOBHm_Chr1g0350731

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007735
Physical Location & Seq
Forward (+)
43893262 .. 43902562
9301 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57656

Sequence Viewer

Length: 5322 bp
ATGGCTTCTTCGGAAGCCGATTCCCGTCTCCGACAGGTCGTTTCTCCAGCCCTAGAGAAGATCATCAAGAACGCCTCCTGGCGCAAGCACGCCAAGCTCGCCAACGAATGCAAGGCCGTCATCGAGCGCCTCTCCAACCCGGACAAGGCGGCCCAGCCCACCGGCCCGGATTCCGAACCCGACGCCTCCGAGCCCGGCCCGCTCCACGACGGAGGCTCCGGTGAGTACTCCCTCGCCGACGCCGAGTCGATTCTCAGCCCTCTCATCGGCGCCGCAGCATCGGGAGTGCTGAAGATCGCCGATCCCGCCGTCGATTGCATCCAGAAGCTCATCGCGCATGGCTACCTGCGCGGCGAGGCCGACGCCTCCGGTGGCGCCGAGGCCAAGTTGCTGACGAAATTGATCGAGTCCGTTTGTAAATGCCACGATTTGGGGGACGATCAGATGGAGCTGCTGGTGCTGAAGACGCTGCTGTCAGCAGTGACGTCGATTTCGCTGCGGATTCACGGCGATTGCTTGCTTCAGATAGTGAGGACTTGCTACGATATCTATTTGGGGAGTAAGAATATAGTGAATCAGACGACGGCAAAGGCCTCGCTGATCCAGATGCTGGTGATTGTGTTCCGGCGAATGGAGGCGGACTCTTCTACGGTGCCGATTAATCCCATTGTGGTGGCGGAATTGATGGATCCCATTGAGAAATCTGATGCTGATGGCTCCATGACTATGTTCGTACAGGGGTTTATTACCAAAATTATGTCTGACATTGATGGGGTGTTGAATCCGACGACGCCTACTAAGCTTTCCGGGCATGATGGGGCGTTCGAGACCACCACGGTGGAGACCACGAATCCGGCGGACTTGCTGGACTCGACTGATAAGGATATGTTGGATGCCAAGTACTGGGAAATTAGTATGTACAAGACGGCATTGGAGGGCCGAAAGGGAGAGCTGGCAGATGGGGAAGTAGAGAGAGATGAAGATTTGGAGGTTCAGATTGGTAATAAGTTGCGCAGAGATGCTTTTTTGGTGTTTAGAGCTCTTTGTAAGCTGTCTATGAAGACCCCGCCCAAGGAGGCATTGGCGGATCCGGAGTTGATGAAGGGAAAGATTGTGGCTTTGGAGCTTTTGAAGATTTTGTTGGAGAATGCCGGTGCTGTGTTTAGGACCAGCGACAGGTTTTTAGGTGCAATTAAGCAATACTTATGTCTGTCATTGTTAAAGAACAGCGCGTCAACTCTTATGATAGTTTTTCAGCTATCTTGTTCCATCTTCATTAGTCTGGTGTCAAGATTTAGAGCTGGATTGAAAGCAGAGATTGGAGTATTCTTTCCTATGATTGTTCTCAGAGTTTTAGAGAATGTTGCACAACCTAATTTTCAGCAGAAGATGATAGTGCTTCGGTTTCTTGAGAAGCTCTGTGTTGATTCCCAGATCTTGGTCGACATATTTATCAATTATGATTGCGATGTCAATTCATCAAACATATTTGAGAGGATGGTAAATGGCCTTCTCAAAACTGCCCAAGGTGTCCCTCCTGGTGTTGCCACCACGTTATTACCTCCTCAAGAGGCAAATATGAAACTTGAAGCCATGAGGTGTTTAGTTGCTATTCTGAGGTCAATCGGGGACTGGATGAACAAACAATTGCGCATTCCAGATCCTCATTCTACCAAGAAATCTGAAGTAAATGAGAATAGTTCTGAACCAGGAGCTCTTCCCATGGCAAATGGGAATGGCGAGGAGTCTGTTGAAGGATCAGATTCTCATTCTGAAGCCTCCAGTGAAGCTTCTGATGCTTTGACAATCGAGCAACGCCGAGCTTACAAGCTGGAACTTCAGGAAGGTATATCTCTTTTCAATCGGAAACCTAAGAAAGGAATTGAGTTTCTTATCAATGCAAATAAGGTGGGCAATTCACCCGAGGAGATAGCTGCTTTTCTAAAAAATGCATCTGGTTTGAACAAGACTTTGATTGGAGACTACCTTGGAGAAAGAGAAGACTTATCGCTAAAAGTAATGCATGCTTATGTGGATTCCTTTGATTTTCAAGACTTGGAGTTTGATGAGGCAATCAGGTCCTTTCTTCAAGGTTTTAGGTTGCCTGGTGAGGCACAGAAGATTGACAGAATAATGGAAAAGTTTGCTGAGCGCTATTGCAAGTGCAATCCAAAGGCTTTTACTAGTGCTGATACAGCCTATGTCCTTGCTTACTCCGTGATACTGCTCAATACTGATGCTCATAACCCGATGGTGAAGAGCAAGATGTCAGCTGATGATTTTATAAGAAACAATCGTGGCATAGATGATGGAAAAGATCTACCTGAGCAGTACTTGAGGTCATTGTATGAACGAATATCAAGAAAGGAAATAAAAATGAAAGAATATGATTTGGCTCCTCAGCAGATACAGTCGGTGAACCCTAACCGTCTTTTGGGCTTGGATAGTATCTTGAATATTGTGATCCGTAAACGGGGGGATAGTCAATTGGAAACCAGTGATGATCTTATCAAGCATATGCAAGAGCAATTCCAAGAAAAAGCTCGGAAATCTGAGTCAGTTTACTATGCTGCAACAGATGTGGTAATTCTTAGATTCATGGTTGAAGTCTGCTGGGCTCCTATGCTGGCTGCCTTCAGTGTTCCACTTGACCAAACTGATGATGAAGTAGTTATTTCTCTGTGTCTTGAAGGGATTCGTTATGCTATCCATGTAACTGCCGCAATGTCCATGAAGACCCATAGAGATGCTTTTGTGACTTCACTGGCAAAGTTTACTTCCCTCCACTCTCCTGCTGATATCAAGCAGAAAAACATAGAAGCAATCAAGGCAATAGTGACAATTGCAGATGAAGATGGGAACTACCTGCAAGAAGCTTGGGAGCATATTTTGACATGTGTTTCCCGCTTTGAACATCTTCATCTCCTGGGAGAGGGTGCTCCTCCAGATGCTACTTTCTTTGCTTTTCCTCAGAATGAATCAGAAAAATCAAAGCAAGCCAAGTCAACTGTCCTTCCTGTTTTGAAAAAGAAGGGCCAAGGGAAGATGCAGTATGCTGCTGCTGCCGTGTTGAGAGGTTCATATGATAGTGCTGGTATTGGTGGGAATTCTTCTGGGATGGTCACATCAGAACAGATGAACAATTTGGTTTCTAACTTAAATATGTTAGAACAAGTTGGAGACATGAGCCGCATATTCACACGGAGTCAAAAGCTGAACAGTGAGGCAATTATAGATTTTGTTAAGGCTCTTTGCAAGGTCTCTATGGAGGAATTGCGATCTGCATCTGATCCTCGGGTTTTCAGCCTTACGAAGATGGTTGAGATTGCGCATTATAACATGAACCGCATCAGGCTTGTGTGGTCTAGCATCTGGCATGTACTCTCTAATTTCTTCGTGACAATTGGCTGTTCTGAAAACCTGTCTATTGCAATTTTTGCTATGGATTCTCTACGCCAATTGTCAATGAAATTCTTGGAGCGAGAAGAGCTGGCTAATTATAATTTTCAGAATGAGTTTATGAAACCTTTTGTCATTGTTATGCGTAAAAGTAGTGCAGTTGAGATACGAGAATTAATCATCAGATGTGTCTCCCAAATGGTTTTATCTCGTGTGAATAGAGTCAAATCTGGATGGAAGAGCATGTTCATGGTCTTCACTACGGCAGCTTATGATGACCACAAAAATATTGTACTCTTGGCCTTTGAAATAATTGAGAAGATTATTCGCGACTACTTTCCGTACATCACCGAGACTGAAACCACTACTTTTACTGATTGCGTAAATTGTCTAATTGCATTCACCAATAGTAGATTTAACAAAGACATTAGCCTCAATGCTATTGCTTTTCTTCGGTTCTGTGCAACAAAACTGGCTGATGGAGGTCTTAGTTCCTCATCAAGAAATAAGGACAAGGATGCATCTGGAAAAATATCTCCAACGTCACCTCAAGGATGGAAAGAAGGAAGACAAGATAATGGGGGAATGCCAGACAAGGATGACCATCTTTATTTCTGGTTCCCTTTATTGGCTGGTTTGTCCGAACTTAGCTTTGATCCTAGGCCTGAAATCAGGAAGAGTGCCTTACAAGTGCTGTTTGAAACTCTACGTAACCATGGTCACCTTTTCTCTCTACCTTTGTGGGAAAAGGTGTTTGAATCAGTGCTATTTCCAATATTTGACTATGTGCGCCATGCTATTGATCCTTCTGGGGAAAGTTCACCAGAGCAAGGGATTGACAGTGAAACAGGCGAGCTTGATCAAGATGCCTGGATGTACGAGACATGCACATTGGCACTCCAATTAGTTGTTGATCTTTTTGTCAAGTTTTATGACACTGTCAATCCTCTTTTGAAGAAAGTTTTGGATCTTCTAGTCAGTTTTATTGGACGACCTCACCAAAGCCTAGCTGGCATAGGTATTGCTGCATTTGTGCGATTGATGAGCAATGCGGGGGATCTCTTTTCTGATGAGAAGTGGCTTGAAGTGGTTTCATCATTAAAAGAAGCTGCCAATTCAACTCTTCCTGATTTTTCTTTCATTCTTAACGGAGATAGCATCATGGGAATCCTAGACTCTTCTTCTAGTAGAGAAGATCATGGAGGGTCTGGGAGGCCTGATGATGATTCAGAGAGACTAAGGACAAACCATCTTTATGCTGGCTTGGCTGACGTTAAGTGCCGAGCTGCTGTTCAGCTATTATTGATTCAGGCAGTGATGGAGATTTATACCATGTATCGTTCTCACCTTTCATCGAATAACACATTAATCCTCTTCAATGCTCTGCATGATTTGGCATCCCATGCTCACAAAGTCAACACTGATACCACTTTACGGGCAAGGCTACAAGAGTTTGGCTCCATGACGCAAATGCAAGACCCTCCACTGTTACGTATTGAGAACGAATCTTACCAAATCTGCCTTACATTCTTACAGAATCTTGTAGAGGACAGGCCTCCAAGTTATGATGAGGTGGAGGTAGAGTCCCACATTGTGGACCTTTGCAAGGAGGTTTTACAGTTCTATATTGAAGCCGCTAGCTCTGGGAAGATATCTGAATCATCTAATGGGCAGCAGCACCATTGGCTGATCCCTCTAGGTTCTGGGCGACGGAGAGAATTGGCTGCACGCGCACCGCTTATTGTGGCGACTCTCCAGGCCATCTGTTGCTTGGGAGACACTTCATTTGAAAATAACTTGTCTCATTTCTTCCCGCTGCTTGCAACCTTGATAACTTGTGAACATGGGTCCGATGAGGTCCAGGCTGCCCTTAGTGACATGCTCAGCTCATCTGTGGGTCCTGTTTTGCTTCGATCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1773

Amino Acids

197.2

Weight (kDa)

5.33

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 6 - 214 1.2e-37 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 338 - 496 1.7e-47 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 610 - 791 6.1e-78 Sec7 domain
Sec7-like_HDS PF09324 1157 - 1239 1.7e-29 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1329 - 1417 2.7e-12 C-terminal region of Mon2 protein
BIG2_C PF20252 1563 - 1762 4.9e-43 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2285, 3336, 3501
AasI GACNNNNNNGTC 1 cut(s) 472
AatII GACGTC 1 cut(s) 488
Acc16I TGCGCA 3 cut(s) 1013, 1652, 3330
Acc36I ACCTGC 2 cut(s) 354, 2874
AccB1I GGYRCC 3 cut(s) 269, 374, 652
AccB7I CCANNNNNTGG 5 cut(s) 430, 610, 903, 1438, 4993
AccBSI CCGCTC 1 cut(s) 202
AccI GTMKAC 1 cut(s) 1443
AccII CGCG 5 cut(s) 335, 351, 1232, 3729, 5130
AccIII TCCGGA 1 cut(s) 1090
AcsI RAATTY 2 cut(s) 3106, 3470
AcuI CTGAAG 7 cut(s) 311, 482, 506, 1704, 1794, 1823, 2620
AcyI GRCGYC 7 cut(s) 183, 240, 270, 363, 375, 485, 791
AdeI CACNNNGTG 1 cut(s) 4993
AfaI GTAC 9 cut(s) 227, 735, 902, 920, 2333, 3381, 3693, 3743, 4277
AfeI AGCGCT 1 cut(s) 2153
AflIII ACRYGT 1 cut(s) 2894
AhdI GACNNNNNGTC 1 cut(s) 244
AhlI ACTAGT 1 cut(s) 2183
AjnI CCWGG 8 cut(s) 77, 1537, 1708, 2104, 2925, 4268, 5154, 5259
AjuI GAANNNNNNNTTGG 4 cut(s) 1124, 1156, 4346, 4378
AleI CACNNNNGTG 1 cut(s) 836
Alw21I GWGCWC 3 cut(s) 1042, 1717, 2941
AlwNI CAGNNNCTG 1 cut(s) 610
Ama87I CYCGRG 2 cut(s) 1922, 3294
Aor13HI TCCGGA 1 cut(s) 1090
Aor51HI AGCGCT 1 cut(s) 2153
ApoI RAATTY 2 cut(s) 3106, 3470
ArsI GACNNNNNNTTYG 6 cut(s) 475, 507, 2045, 2077, 5168, 5200
AseI ATTAAT 3 cut(s) 660, 3575, 4766
Asp700I GAANNNNTTC 2 cut(s) 2694, 2916
AspA2I CCTAGG 1 cut(s) 4058
AsuC2I CCSGG 4 cut(s) 140, 167, 195, 808
AsuNHI GCTAGC 1 cut(s) 5036
AvaI CYCGRG 2 cut(s) 1922, 3294
AvaII GGWCC 6 cut(s) 1167, 2079, 4996, 5247, 5257, 5297
AvrII CCTAGG 1 cut(s) 4058
BamHI GGATCC 2 cut(s) 688, 1087
BanI GGYRCC 3 cut(s) 269, 374, 652
BanII GRGCYC 4 cut(s) 195, 1042, 1717, 2620
BarI GAAGNNNNNNTAC 2 cut(s) 1494, 1526
BauI CACGAG 1 cut(s) 3609
BbsI GAAGAC 6 cut(s) 470, 1067, 2007, 2741, 3646, 3973
Bbv12I GWGCWC 3 cut(s) 1042, 1717, 2941
BbvCI CCTCAGC 1 cut(s) 2400
BceAI ACGGC 7 cut(s) 101, 293, 523, 600, 942, 3050, 3678
BcgI CGANNNNNNTGC 6 cut(s) 468, 478, 502, 512, 576, 610
BciT130I CCWGG 8 cut(s) 79, 1539, 1710, 2106, 2927, 4270, 5156, 5261
BclI TGATCA 1 cut(s) 4258
BcnI CCSGG 4 cut(s) 140, 167, 195, 808
BcuI ACTAGT 1 cut(s) 2183
BfoI RGCGCY 4 cut(s) 130, 273, 378, 2155
BfuAI ACCTGC 2 cut(s) 354, 2874
BglI GCCNNNNNGGC 1 cut(s) 4410
BglII AGATCT 2 cut(s) 1434, 2317
BlnI CCTAGG 1 cut(s) 4058
BlpI GCTNAGC 2 cut(s) 2148, 5282
BmcAI AGTACT 3 cut(s) 227, 902, 2333
Bme18I GGWCC 6 cut(s) 1167, 2079, 4996, 5247, 5257, 5297
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 2 cut(s) 1922, 3294
BmrI ACTGGG 1 cut(s) 913
BmtI GCTAGC 1 cut(s) 5040
BmuI ACTGGG 1 cut(s) 913
BpiI GAAGAC 6 cut(s) 470, 1067, 2007, 2741, 3646, 3973
BplI GAGNNNNNCTC 6 cut(s) 116, 148, 626, 658, 4841, 4873
BpmI CTGGAG 4 cut(s) 30, 1765, 2928, 5138
Bpu10I CCTNAGC 2 cut(s) 2325, 2400
Bpu1102I GCTNAGC 2 cut(s) 2148, 5282
BpuEI CTTGAG 4 cut(s) 1430, 1551, 2356, 3933
BpuMI CCSGG 4 cut(s) 140, 167, 195, 808
BsaAI YACGTR 2 cut(s) 4109, 4892
BsaBI GATNNNNATC 2 cut(s) 3283, 4002
BsaHI GRCGYC 7 cut(s) 183, 240, 270, 363, 375, 485, 791
BsaI GGTCTC 3 cut(s) 821, 836, 3265
BsaWI WCCGGW 3 cut(s) 218, 368, 1090
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 4967, 4997
Bse118I RCCGGY 2 cut(s) 161, 1151
Bse1I ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 3876
Bse3DI GCAATG 2 cut(s) 2730, 4453
Bse8I GATNNNNATC 2 cut(s) 3283, 4002
BseAI TCCGGA 1 cut(s) 1090
BseBI CCWGG 8 cut(s) 79, 1539, 1710, 2106, 2927, 4270, 5156, 5261
BseJI GATNNNNATC 2 cut(s) 3283, 4002
BseMI GCAATG 2 cut(s) 2730, 4453
BseMII CTCAG 9 cut(s) 268, 1360, 1607, 2139, 2316, 2414, 2544, 2984, 5296
BseNI ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 3876
BseRI GAGGAG 5 cut(s) 1554, 1757, 1940, 2388, 2931
BseYI CCCAGC 2 cut(s) 153, 2613
BsgI GTGCAG 2 cut(s) 3576, 5109
Bsh1236I CGCG 5 cut(s) 335, 351, 1232, 3729, 5130
BshNI GGYRCC 3 cut(s) 269, 374, 652
BsiHKAI GWGCWC 3 cut(s) 1042, 1717, 2941
BsiHKCI CYCGRG 2 cut(s) 1922, 3294
BslFI GGGAC 4 cut(s) 449, 1517, 1643, 4969
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 4 cut(s) 449, 1517, 1643, 4969
BsmI GAATGC 5 cut(s) 113, 1153, 1653, 3797, 3990
Bso31I GGTCTC 3 cut(s) 821, 836, 3265
BsoBI CYCGRG 2 cut(s) 1922, 3294
Bsp1286I GDGCHC 5 cut(s) 195, 1042, 1717, 2620, 2941
Bsp13I TCCGGA 1 cut(s) 1090
Bsp1407I TGTACA 1 cut(s) 918
Bsp1720I GCTNAGC 2 cut(s) 2148, 5282
Bsp19I CCATGG 2 cut(s) 1722, 4114
Bsp68I TCGCGA 1 cut(s) 3729
BspCNI CTCAG 9 cut(s) 267, 1359, 1608, 2140, 2317, 2413, 2545, 2983, 5295
BspEI TCCGGA 1 cut(s) 1090
BspFNI CGCG 5 cut(s) 335, 351, 1232, 3729, 5130
BspMI ACCTGC 2 cut(s) 354, 2874
BspOI GCTAGC 1 cut(s) 5040
BspQI GCTCTTC 4 cut(s) 1722, 2252, 3480, 3632
BspT107I GGYRCC 3 cut(s) 269, 374, 652
BspTNI GGTCTC 3 cut(s) 821, 836, 3265
BsrBI CCGCTC 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 2730, 4453
BsrFI RCCGGY 2 cut(s) 161, 1151
BsrGI TGTACA 1 cut(s) 918
BsrI ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 3876
BssAI RCCGGY 2 cut(s) 161, 1151
BssNI GRCGYC 7 cut(s) 183, 240, 270, 363, 375, 485, 791
BssSI CACGAG 1 cut(s) 3609
BssT1I CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
Bst2BI CACGAG 1 cut(s) 3609
Bst2UI CCWGG 8 cut(s) 79, 1539, 1710, 2106, 2927, 4270, 5156, 5261
Bst6I CTCTTC 9 cut(s) 649, 1722, 2252, 3480, 3632, 4070, 4527, 4582, 4778
BstACI GRCGYC 7 cut(s) 183, 240, 270, 363, 375, 485, 791
BstAPI GCANNNNNTGC 2 cut(s) 3437, 4802
BstAUI TGTACA 1 cut(s) 918
BstBAI YACGTR 2 cut(s) 4109, 4892
BstDSI CCRYGG 3 cut(s) 834, 1722, 4114
BstEII GGTNACC 1 cut(s) 4118
BstENI CCTNNNNNAGG 3 cut(s) 1875, 2930, 5003
BstFNI CGCG 5 cut(s) 335, 351, 1232, 3729, 5130
BstH2I RGCGCY 4 cut(s) 130, 273, 378, 2155
BstNI CCWGG 8 cut(s) 79, 1539, 1710, 2106, 2927, 4270, 5156, 5261
BstNSI RCATGY 6 cut(s) 2027, 2898, 3380, 3646, 4287, 5281
BstPI GGTNACC 1 cut(s) 4118
BstSNI TACGTA 2 cut(s) 4109, 4892
BstUI CGCG 5 cut(s) 335, 351, 1232, 3729, 5130
BstV2I GAAGAC 6 cut(s) 470, 1067, 2007, 2741, 3646, 3973
BstX2I RGATCY 7 cut(s) 688, 1087, 1434, 1660, 2317, 4366, 4456
BstXI CCANNNNNNTGG 2 cut(s) 673, 838
BstYI RGATCY 7 cut(s) 688, 1087, 1434, 1660, 2317, 4366, 4456
BtgI CCRYGG 3 cut(s) 834, 1722, 4114
BtgZI GCGATG 2 cut(s) 316, 1482
BtsI GCAGTG 2 cut(s) 486, 4719
BtuMI TCGCGA 1 cut(s) 3729
BveI ACCTGC 2 cut(s) 354, 2874
CaiI CAGNNNCTG 1 cut(s) 610
Cfr10I RCCGGY 2 cut(s) 161, 1151
CseI GACGC 7 cut(s) 191, 248, 371, 475, 799, 1221, 4873
Csp6I GTAC 9 cut(s) 226, 734, 901, 919, 2332, 3380, 3692, 3742, 4276
CspCI CAANNNNNGTGG 4 cut(s) 1890, 1925, 2562, 2597
CviQI GTAC 9 cut(s) 226, 734, 901, 919, 2332, 3380, 3692, 3742, 4276
DinI GGCGCC 2 cut(s) 271, 376
DraIII CACNNNGTG 1 cut(s) 4993
DrdI GACNNNNNNGTC 1 cut(s) 472
DriI GACNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 472
Eam1104I CTCTTC 9 cut(s) 649, 1722, 2252, 3480, 3632, 4070, 4527, 4582, 4778
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 9 cut(s) 649, 1722, 2252, 3480, 3632, 4070, 4527, 4582, 4778
EciI GGCGGA 4 cut(s) 653, 692, 872, 1100
Ecl136II GAGCTC 2 cut(s) 1040, 1715
Eco105I TACGTA 2 cut(s) 4109, 4892
Eco130I CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
Eco147I AGGCCT 4 cut(s) 593, 4063, 4615, 4954
Eco24I GRGCYC 4 cut(s) 195, 1042, 1717, 2620
Eco31I GGTCTC 3 cut(s) 821, 836, 3265
Eco32I GATATC 3 cut(s) 547, 2800, 5052
Eco47I GGWCC 6 cut(s) 1167, 2079, 4996, 5247, 5257, 5297
Eco47III AGCGCT 1 cut(s) 2153
Eco53kI GAGCTC 2 cut(s) 1040, 1715
Eco57I CTGAAG 7 cut(s) 311, 482, 506, 1704, 1794, 1823, 2620
Eco88I CYCGRG 2 cut(s) 1922, 3294
Eco91I GGTNACC 1 cut(s) 4118
EcoICRI GAGCTC 2 cut(s) 1040, 1715
EcoNI CCTNNNNNAGG 3 cut(s) 1875, 2930, 5003
EcoO109I RGGNCCY 2 cut(s) 2079, 5297
EcoO65I GGTNACC 1 cut(s) 4118
EcoRI GAATTC 1 cut(s) 3106
EcoRII CCWGG 8 cut(s) 77, 1537, 1708, 2104, 2925, 4268, 5154, 5259
EcoRV GATATC 3 cut(s) 547, 2800, 5052
EcoT14I CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
EcoT22I ATGCAT 3 cut(s) 1954, 2025, 3922
EcoT38I GRGCYC 4 cut(s) 195, 1042, 1717, 2620
EgeI GGCGCC 2 cut(s) 271, 376
EheI GGCGCC 2 cut(s) 271, 376
ErhI CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
Esp3I CGTCTC 1 cut(s) 32
FalI AAGNNNNNCTT 4 cut(s) 1187, 1219, 4067, 4099
FaqI GGGAC 4 cut(s) 449, 1517, 1643, 4969
FauI CCCGC 6 cut(s) 207, 313, 1074, 2912, 4444, 5220
FauNDI CATATG 2 cut(s) 2517, 3082
FbaI TGATCA 1 cut(s) 4258
FblI GTMKAC 1 cut(s) 1443
FriOI GRGCYC 4 cut(s) 195, 1042, 1717, 2620
FspI TGCGCA 3 cut(s) 1013, 1652, 3330
GsaI CCCAGC 2 cut(s) 157, 2617
GsuI CTGGAG 4 cut(s) 30, 1765, 2928, 5138
HaeII RGCGCY 4 cut(s) 130, 273, 378, 2155
HgaI GACGC 7 cut(s) 191, 248, 371, 475, 799, 1221, 4873
Hin1I GRCGYC 7 cut(s) 183, 240, 270, 363, 375, 485, 791
HincII GTYRAC 4 cut(s) 1236, 1444, 3006, 4816
HindII GTYRAC 4 cut(s) 1236, 1444, 3006, 4816
HindIII AAGCTT 3 cut(s) 800, 1788, 2874
HpyCH4IV ACGT 6 cut(s) 485, 1553, 3941, 4108, 4671, 4891
HpySE526I ACGT 6 cut(s) 485, 1553, 3941, 4108, 4671, 4891
Hsp92I GRCGYC 7 cut(s) 183, 240, 270, 363, 375, 485, 791
KasI GGCGCC 2 cut(s) 269, 374
Kpn2I TCCGGA 1 cut(s) 1090
Ksp22I TGATCA 1 cut(s) 4258
LguI GCTCTTC 4 cut(s) 1722, 2252, 3480, 3632
MaeII ACGT 6 cut(s) 485, 1553, 3941, 4108, 4671, 4891
MbiI CCGCTC 1 cut(s) 202
MfeI CAATTG 5 cut(s) 1646, 2486, 2841, 3402, 3458
MflI RGATCY 7 cut(s) 688, 1087, 1434, 1660, 2317, 4366, 4456
MhlI GDGCHC 5 cut(s) 195, 1042, 1717, 2620, 2941
Mly113I GGCGCC 2 cut(s) 270, 375
MmeI TCCRAC 7 cut(s) 55, 159, 809, 870, 1122, 3157, 3962
Mph1103I ATGCAT 3 cut(s) 1954, 2025, 3922
MroI TCCGGA 1 cut(s) 1090
MroXI GAANNNNTTC 2 cut(s) 2694, 2916
MslI CAYNNNNRTG 8 cut(s) 671, 725, 836, 2028, 2745, 3539, 3647, 4653
MspA1I CMGCKG 2 cut(s) 2273, 5215
MunI CAATTG 5 cut(s) 1646, 2486, 2841, 3402, 3458
Mva1269I GAATGC 5 cut(s) 113, 1153, 1653, 3797, 3990
MvaI CCWGG 8 cut(s) 79, 1539, 1710, 2106, 2927, 4270, 5156, 5261
MvnI CGCG 5 cut(s) 335, 351, 1232, 3729, 5130
NarI GGCGCC 2 cut(s) 270, 375
NciI CCSGG 4 cut(s) 140, 167, 195, 808
NcoI CCATGG 2 cut(s) 1722, 4114
NdeI CATATG 2 cut(s) 2517, 3082
NheI GCTAGC 1 cut(s) 5036
NmeAIII GCCGAG 4 cut(s) 268, 403, 1844, 4706
NmuCI GTSAC 8 cut(s) 481, 2755, 2836, 3121, 3397, 3942, 4118, 5273
NruI TCGCGA 1 cut(s) 3729
NsbI TGCGCA 3 cut(s) 1013, 1652, 3330
NsiI ATGCAT 3 cut(s) 1954, 2025, 3922
NspI RCATGY 6 cut(s) 2027, 2898, 3380, 3646, 4287, 5281
OliI CACNNNNGTG 1 cut(s) 836
PaeI GCATGC 1 cut(s) 2027
PceI AGGCCT 4 cut(s) 593, 4063, 4615, 4954
PciI ACATGT 1 cut(s) 2894
PciSI GCTCTTC 4 cut(s) 1722, 2252, 3480, 3632
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 5 cut(s) 113, 1153, 1653, 3797, 3990
PdmI GAANNNNTTC 2 cut(s) 2694, 2916
PflFI GACNNNGTC 1 cut(s) 4337
PflMI CCANNNNNTGG 5 cut(s) 430, 610, 903, 1438, 4993
PluTI GGCGCC 2 cut(s) 273, 378
Ppu21I YACGTR 2 cut(s) 4109, 4892
PpuMI RGGWCCY 2 cut(s) 2079, 5297
PscI ACATGT 1 cut(s) 2894
PshBI ATTAAT 3 cut(s) 660, 3575, 4766
PsiI TTATAA 3 cut(s) 2285, 3336, 3501
Psp124BI GAGCTC 2 cut(s) 1042, 1717
Psp5II RGGWCCY 2 cut(s) 2079, 5297
Psp6I CCWGG 8 cut(s) 77, 1537, 1708, 2104, 2925, 4268, 5154, 5259
PspEI GGTNACC 1 cut(s) 4118
PspFI CCCAGC 2 cut(s) 153, 2613
PspGI CCWGG 8 cut(s) 77, 1537, 1708, 2104, 2925, 4268, 5154, 5259
PspPPI RGGWCCY 2 cut(s) 2079, 5297
PsrI GAACNNNNNNTAC 2 cut(s) 4892, 4924
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 7 cut(s) 688, 1087, 1434, 1660, 2317, 4366, 4456
PsyI GACNNNGTC 1 cut(s) 4337
PvuII CAGCTG 1 cut(s) 2273
RruI TCGCGA 1 cut(s) 3729
RsaI GTAC 9 cut(s) 227, 735, 902, 920, 2333, 3381, 3693, 3743, 4277
RsaNI GTAC 9 cut(s) 226, 734, 901, 919, 2332, 3380, 3692, 3742, 4276
RseI CAYNNNNRTG 8 cut(s) 671, 725, 836, 2028, 2745, 3539, 3647, 4653
SacI GAGCTC 2 cut(s) 1042, 1717
SalI GTCGAC 1 cut(s) 1442
SapI GCTCTTC 4 cut(s) 1722, 2252, 3480, 3632
ScaI AGTACT 3 cut(s) 227, 902, 2333
SduI GDGCHC 5 cut(s) 195, 1042, 1717, 2620, 2941
SfoI GGCGCC 2 cut(s) 271, 376
SinI GGWCC 6 cut(s) 1167, 2079, 4996, 5247, 5257, 5297
SmiMI CAYNNNNRTG 8 cut(s) 671, 725, 836, 2028, 2745, 3539, 3647, 4653
SmlI CTYRAG 4 cut(s) 1409, 1566, 2335, 3948
SmoI CTYRAG 4 cut(s) 1409, 1566, 2335, 3948
SnaBI TACGTA 2 cut(s) 4109, 4892
SpeI ACTAGT 1 cut(s) 2183
SphI GCATGC 1 cut(s) 2027
SseBI AGGCCT 4 cut(s) 593, 4063, 4615, 4954
SspDI GGCGCC 2 cut(s) 269, 374
SspI AATATT 3 cut(s) 2458, 3688, 4176
SstI GAGCTC 2 cut(s) 1042, 1717
StuI AGGCCT 4 cut(s) 593, 4063, 4615, 4954
StyI CCWWGG 7 cut(s) 1071, 1525, 1722, 1987, 3037, 4058, 4114
TaiI ACGT 6 cut(s) 488, 1556, 3944, 4111, 4674, 4894
TatI WGTACW 6 cut(s) 225, 900, 918, 2331, 3379, 3691
TauI GCSGC 6 cut(s) 152, 275, 354, 2723, 3192, 5036
TseFI GTSAC 8 cut(s) 481, 2755, 2836, 3121, 3397, 3942, 4118, 5273
Tsp45I GTSAC 8 cut(s) 481, 2755, 2836, 3121, 3397, 3942, 4118, 5273
TspGWI ACGGA 8 cut(s) 225, 400, 2206, 2456, 3217, 3729, 4563, 5125
Tth111I GACNNNGTC 1 cut(s) 4337
Van91I CCANNNNNTGG 5 cut(s) 430, 610, 903, 1438, 4993
VpaK11BI GGWCC 6 cut(s) 1167, 2079, 4996, 5247, 5257, 5297
VspI ATTAAT 3 cut(s) 660, 3575, 4766
XagI CCTNNNNNAGG 3 cut(s) 1875, 2930, 5003
XapI RAATTY 2 cut(s) 3106, 3470
XceI RCATGY 6 cut(s) 2027, 2898, 3380, 3646, 4287, 5281
XcmI CCANNNNNNNNNTGG 4 cut(s) 1078, 4406, 4655, 5167
XmaJI CCTAGG 1 cut(s) 4058
XmiI GTMKAC 1 cut(s) 1443
XmnI GAANNNNTTC 2 cut(s) 2694, 2916
ZraI GACGTC 1 cut(s) 486
ZrmI AGTACT 3 cut(s) 227, 902, 2333
Zsp2I ATGCAT 3 cut(s) 1954, 2025, 3922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.