FvH4_7g12020

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007735
Physical Location & Seq
Forward (+)
10929209 .. 10939661
10453 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12020.t1

Sequence Viewer

Length: 5322 bp
ATGGCTTCTTCGGAAGCCGATTCCCGTCTCCGACAGGTCGTTTCTCCAGCCCTAGAGAAGATCATCAAGAACGCCTCCTGGCGCAAGCACGCCAAGCTCGCCAGCGAATGCAAGGCCGTCATCGACCGCCTCTCCAACCCTGACAAGGCCTCCCACCCCGCCAGCCCAAATTCCGAGCCCGAAGCCTCCGAGCCCGGCCCTCTCCACGACGGCGGTTCCGATGAGTACTCCCTCGCCGACGCGGAGTCGATTCTCAGCCCTATCATCAACGCCGCCGCGTCCGGTGTGCTGAAGATCGCCGATCCCGCCGTGGATTGCATCCAGAAGCTAATCGCGCACGGATACCTGCGCGGCGAGGCCGACACTTCCGGTGGCGCCGAGGCCAAGTTGCTGACGAAGCTGATCGAGTCGGTTTGTAAATGCCATGATTTGGGAGACGATCAGATGGAGCTGCTGGTGCTGAAGACGCTGCTGTCAGCAGTGACATCGATTTCGCTGCGGATTCACGGCGACTGCTTGCTTCAGATAGTTAGGACTTGCTATGATATCTATTTGGGGAGTAAGAATATAGTGAATCAGACGACGGCGAAGGCGTCTCTGATCCAGATGCTGGTGATTGTGTTCCGGCGAATGGAGGCTGACTCTTCGACGGTGCCGATTAATCCCATAGTGGTGGCGGAGTTGATGGATCCCGTTGAGAAATCTGACGCTGACGCGTCCATGACTATGTTTGTGCAAGGGTTTATTACCAAGATTATGTCAGACATTGATGGGGTGTTGAATCCCACGACGCCTACTAAGCTTTCCAAGCATGATGGGGCGTTTGAGACCACCACGGTGGAGACCACGAATCCGGCGGATTTGCTGGACTCCACTGATAAGGATATGTTGGATGCCAAGTACTGGGAAATTAGTATGTATAAGACGGCATTGGAAGGCCGAAAGGGAGAGCTGGCGGATGGGGAAATGGAGAGGGATGAAGATTTGGAGGTTCAGATTGGTAATAAGTTGCGGAGAGATGCTTTTTTGGTGTTTAGAGCTCTTTGTAAGCTGTCTATGAAGACTCCTCCTAAGGAGGCATTGGCGGATCCAGAGTTGATGAAGGGAAAAATTGTGGCTTTGGAGCTTTTGAAGATTTTGTTGGAGAATGCCGGGGCTGTTTTTAGGACCAGCGACAGGTTTTTAGGTGCAATTAAGCAATACTTATGTCTGTCATTGCTAAAGAACAGTGCGTCAACTCTTATGATAGTTTTTCAGCTATCTTGTTCCATCTTCATTAGTCTGGTGTCAAGATTTAGAGCTGGATTGAAAGCAGAGATTGGAGTATTTTTTCCTATGATTGTTCTCAGAGTTTTAGAGAATGTTGCACAACCTAATTTTCAGCAGAAGATGATAGTGCTTCGGTTTCTTGAGAAGCTCTGTGTTGATTCTCAGATCTTGGTAGACATATTTATCAACTATGATTGTGATGTCAATTCATCAAACATATTTGAAAGGATGGTAAATGGCCTTCTTAAAACTGCCCAAGGTGTCCCTCCTGGTGTTGTCACCACGTTATTACCTCCTCAAGAGGCTACTATGAAACTTGAAGCCTTGAGGTGTTTAGTTGGTATTCTGAGGTCAATAGGGGACTGGATGAACAAACAATTGCGCATTCCAGATCCTCATTCTATCAATAAATCTGAGGTGAACGAGAATGGTTCTGAACCAGGAGCTCTACCCATGGCAAACGGGAATGGCGAGGAGCCTGTTGAAGGATCAGATTCTCAATCTGAAGCTTCCAGTGAAGCCTCTGATGCTTTGACAATTGAGCAACGCCGAGCTTACAAGCTGGAACTTCAGGAAGGTATATCTCTTTTCAATCGGAAACCGAAGAAAGGAATCGAGTTTCTTATCAATGCAAATAAGGTGGGTGATTCACCCGAGGAGATAGCTACTTTTCTAAAAAATGCATCTGGTTTGAACAAGACTATGATTGGGGATTATCTTGGAGAAAGAGAAGACTTATCGCTAAAAGTAATGCATGCTTATGTGGAATCCTTTGATTTTCAAAACTTGGAGTTTGATGAGGCAATCAGGTCCTTTCTTCAAGGCTTTAGGTTGCCTGGTGAGGCACAGAAGATCGACCGAATAATGGAAAAGTTCGCTGAGCGCTATTGCAAGTGCAATCCAAAGGCTTTTACTAGTGCTGATACTGCCTATGTGCTTGCTTACTCCGTGATATTGCTCAATACTGATGCTCATAATCCTATGGTGAAGAGCAAGATGTCAGCCGACGATTTTATAAGAAACAATCGTGGCATAGATGATGGAAAAGACCTATCTGAGGAGTACTTGAGGTCATTATATGAACGGATATCAAAGAAGGAGATAAAAATGAAAGATTATGATTTGGCTCCTCAGCAGATACAATCTGTGAACCCAAACCGTCTTTTGGGGTTGGATAGTATCTTGAATATTGTGATCCGTAAACGGGGGGACAGTCAATTGGAGACCAGTGACGATCTTATCAAGCACATGCAAGAACAATTCAAAGAAAAAGCTCGGAAATCTGAGTCAGTTTATTATGCTGCAACAGATGTGGTAATTCTTAGATTCATGGTTGAGGTCTGCTGGGCTCCTATGTTGGCTGCCTTCAGTGTTCCACTTGACCAAACTGATGATGAAGTGGTTATTTCTCTGTGTCTTGAAGGGATTCGTTATGCTATCCATGTAACTGCCGCAATGTCTATGAAGACCCATAGAGATGCTTTTGTGACTTCACTGGCGAAGTTTACTTCCCTCCACTCTCCTGCTGATATCAAGCAGAAAAACATAGAAGCAATCAAGGCAATAGTGACAATAGCAGATGAAGATGGGAACTACCTACAAGAAGCTTGGGAGCATATTTTGACATGTGTTTCCCGCTTTGAGCATCTACATCTCTTGGGAGAGGGTGCTCCTCCAGATGCCACTTTCTTTGCTTTTCCTCAGAATGAATCAGAAAAATCAAAGCAAACCAAGTCCACTGTCCTTCCTGTTTTAAAAAAGAAGGGCCAAGGGAAGATGCAGTATGCAGCTGCTGCCGTGTTGAGAGGTTCATATGATAGTGCTGGTATTGGTGGGAATGCTTCTGGGATGGTTACATCAGAACAGATGAACAATTTGGTTTCTAACTTAAATATGTTAGAACAAGTTGGAGACATGAGCCGCATATTCACACGGAGTCAAAAGCTAAACAGTGAGGCAATTATAGATTTTGTTAAGGCTCTTTGCAAGGTCTCTATGGAGGAATTGCGATCCGCATCTGATCCTCGGGTTTTCAGCCTCACAAAGATGGTTGAAATTGCGCATTATAACATGAACCGCATCAGGCTTGTGTGGTCTAGCATCTGGCATGTACTCTCTAATTTCTTTGTGACAATTGGCTGTTCTGAAAACCTGTCTATTGCAATTTTTGCTATGGATTCTCTGCGTCAATTGTCAATGAAGTTCTTGGAGCGAGAAGAGCTGGCTAATTATAATTTTCAGAATGAATTTATGAAACCTTTCGTCATTGTTATGCGTAAAAGTAGTGCAGTTGAGATTCGAGAATTAATCATCAGATGTGTCTCCCAAATGGTTTTATCTCGTGTCAATAATGTCAAATCTGGATGGAAGAGTATGTTCATGGTCTTCACTACGGCAGCTTATGATGACCACAAGAATATTGTACTCTTGGCCTTTGAAATAATTGAGAAGATTATTCGCGATTACTTTCCGTACATTACTGAGACTGAAACCACTACTTTTACTGATTGCGTAAATTGTCTAATTGCATTCACAAATAGTAGATTCAACAAAGACATTAGCCTCAATGCTATTGCTTTTCTTCGGTTCTGTGCAACAAAACTCGCTGAGGGAGGTCTTAGTTCCTCATCAAGAAATAAGGACAAGGATGCATCTGGAAAAGTCTCTCCGTCTTCTCCTCAAGGATGGAAAGAAGGAAGACAAGATAATGGGGGAATGCCAGATAAGGATGACCATCTTTATTTCTGGTTCCCTTTATTGGCCGGTTTGTCCGAACTTAGCTTTGACCCTAGGCCTGAAATCAGGAAGAGTGCCTTACAAGTGCTATTTGAATCTTTACGTAACCATGGTCATCTTTTCTCTCTACCTTTGTGGGAAAAGGTGTTTGAATCAGTGCTATTTCCAATATTTGACTATGTGCGCCATGCTATTGATCCTTCGGGGGACAGTCCACCAGAGCAAGGGATTGACAGTGAAACAGGCGAGCTTGATCAAGATGCCTGGATGTATGAGACATGCACATTGGCACTCCAATTAGTTGTTGATCTTTTTGTCAAGTTTTATGACACTGTCAATCCTCTCTTGAAGAAAGTTTTGGTGCTTCTAGTCAGTTTTATTGGACGACCTCATCAAAGCCTTGCTGGCATAGGTATTGCTGCATTTGTGAGATTGATGAGCAATGCAGGGGATCTCTTTTCTGATGAGAAGTGGCTAGAAGTGGTTTCATCATTAAAAGAAGCAGCCAATTCAACTCTTCCTGATTTTTCTTTCATTCTTAGTGGAGATAGCATCGTGGCAAACCTAGACTCTTCTTCTAGTAGAGAAGATCATGGAGGGTCTGGGAGGCCTGATGATGAATCAGAGAGACTAAGGACAAACCATCTCTATACTGGCTTAGCTGACGTTAAGTGCCGAGCTGCTGTTCAGCTTTTATTGATTCAGGCGGTGATGGAGATTTACACCATGTATCGGACTCACCTTTCAACGGATAACACATTAATCCTCTTCAATGCTTTGCATGATTTAGCATCACATGCTCACAAAATTAACACTGATACCACTTTACGGGCAAGGCTACAAGAGTTTGGCTCCATGACGCAAATGCAAGACCCTCCACTGTTACGTATTGAGAATGAATCTTACCAAATCTGCCTTACATTCTTACAGAATCTTATAGAGGACAGGCCTCCAAGATTTGATGAGGTGGAGGTAGAGTCCCACGTTGTGGAACTTTGCAAGGAGGTTCTACAGTTCTATATTGAAGCCGCTAGCTCTGGGAAGATATCTGAATCATCTAATGGACAGCAGCACCATTGGCTGATTCCTCTAGGTTCTGGGCGACGGAGAGAATTGGCTGCACGTGCACCGCTTATTGTGGCAACTCTCCAGGCCATCTGTTGCTTGGGAGAGACTTCATTTGAACACAACTTGTCTCATTTCTTCCCACTGCTTGCAACCTTGATAAGTTGTGAACATGGGTCTGATGAGGTCCAGATTGCCCTCAGTGACATGCTCAGCTCATCAGTCGGTCCCGTTCTGCTCCGATCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1774

Amino Acids

197.51

Weight (kDa)

5.36

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 6 - 214 5.7e-38 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 338 - 496 1.7e-47 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 610 - 791 1e-78 Sec7 domain
Sec7-like_HDS PF09324 1157 - 1239 3.7e-30 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1329 - 1399 2.6e-11 C-terminal region of Mon2 protein
BIG2_C PF20252 1563 - 1762 4.5e-42 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2285, 3336, 3501
AasI GACNNNNNNGTC 1 cut(s) 472
Acc16I TGCGCA 2 cut(s) 1652, 3330
Acc36I ACCTGC 1 cut(s) 354
AccB1I GGYRCC 2 cut(s) 374, 652
AccB7I CCANNNNNTGG 4 cut(s) 430, 610, 903, 4993
AccI GTMKAC 1 cut(s) 1443
AccII CGCG 6 cut(s) 242, 278, 335, 351, 716, 3729
AcoI YGGCCR 1 cut(s) 4029
AcsI RAATTY 2 cut(s) 169, 3515
AcuI CTGAAG 6 cut(s) 311, 482, 506, 1794, 1823, 2620
AcvI CACGTG 1 cut(s) 5129
AcyI GRCGYC 3 cut(s) 375, 593, 791
AdeI CACNNNGTG 1 cut(s) 4993
AfaI GTAC 6 cut(s) 227, 902, 2333, 3381, 3693, 3743
AfeI AGCGCT 1 cut(s) 2153
AflIII ACRYGT 2 cut(s) 714, 2894
AhdI GACNNNNNGTC 1 cut(s) 244
AhlI ACTAGT 1 cut(s) 2183
AjnI CCWGG 6 cut(s) 77, 1537, 1708, 2104, 4268, 5154
AjuI GAANNNNNNNTTGG 4 cut(s) 1124, 1156, 4346, 4378
AleI CACNNNNGTG 1 cut(s) 836
Alw21I GWGCWC 4 cut(s) 1042, 1717, 2941, 5134
Alw44I GTGCAC 1 cut(s) 5130
AlwNI CAGNNNCTG 2 cut(s) 610, 3062
Ama87I CYCGRG 2 cut(s) 1922, 3294
Aor51HI AGCGCT 1 cut(s) 2153
ApaLI GTGCAC 1 cut(s) 5130
ApoI RAATTY 2 cut(s) 169, 3515
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AseI ATTAAT 3 cut(s) 660, 3575, 4766
Asp700I GAANNNNTTC 2 cut(s) 2694, 3527
AspA2I CCTAGG 1 cut(s) 4058
AspLEI GCGC 8 cut(s) 84, 337, 351, 377, 1653, 2154, 3331, 4191
AspS9I GGNCC 6 cut(s) 197, 1167, 2079, 3034, 5257, 5297
AsuC2I CCSGG 2 cut(s) 195, 1153
AsuHPI GGTGA 9 cut(s) 625, 1540, 1699, 1911, 1925, 2120, 2266, 4726, 4736
AsuNHI GCTAGC 1 cut(s) 5036
AvaI CYCGRG 2 cut(s) 1922, 3294
AvaII GGWCC 4 cut(s) 1167, 2079, 5257, 5297
AvrII CCTAGG 1 cut(s) 4058
AxyI CCTNAGG 1 cut(s) 1071
BaeGI GKGCMC 1 cut(s) 5134
BamHI GGATCC 2 cut(s) 688, 1087
BanI GGYRCC 2 cut(s) 374, 652
BanII GRGCYC 5 cut(s) 180, 195, 1042, 1717, 2620
BarI GAAGNNNNNNTAC 2 cut(s) 1494, 1526
BauI CACGAG 1 cut(s) 3609
BbrPI CACGTG 1 cut(s) 5129
BbsI GAAGAC 7 cut(s) 470, 1067, 2007, 2741, 3646, 3933, 3973
Bbv12I GWGCWC 4 cut(s) 1042, 1717, 2941, 5134
BbvCI CCTCAGC 2 cut(s) 2400, 3876
BceAI ACGGC 8 cut(s) 101, 226, 293, 523, 600, 942, 3050, 3678
BcgI CGANNNNNNTGC 4 cut(s) 468, 478, 502, 512
BciT130I CCWGG 6 cut(s) 79, 1539, 1710, 2106, 4270, 5156
BciVI GTATCC 1 cut(s) 335
BclI TGATCA 1 cut(s) 4258
BcnI CCSGG 2 cut(s) 195, 1153
BcuI ACTAGT 1 cut(s) 2183
BfmI CTRYAG 1 cut(s) 5015
BfoI RGCGCY 2 cut(s) 378, 2155
BfuAI ACCTGC 1 cut(s) 354
BfuI GTATCC 1 cut(s) 335
BglI GCCNNNNNGGC 1 cut(s) 4410
BglII AGATCT 1 cut(s) 1434
BlnI CCTAGG 1 cut(s) 4058
BlpI GCTNAGC 3 cut(s) 2148, 4663, 5282
BmcAI AGTACT 3 cut(s) 227, 902, 2333
Bme1390I CCNGG 8 cut(s) 79, 195, 1153, 1539, 1710, 2106, 4270, 5156
Bme18I GGWCC 4 cut(s) 1167, 2079, 5257, 5297
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 2 cut(s) 1922, 3294
BmgT120I GGNCC 6 cut(s) 197, 1167, 2079, 3034, 5257, 5297
BmrFI CCNGG 8 cut(s) 79, 195, 1153, 1539, 1710, 2106, 4270, 5156
BmrI ACTGGG 1 cut(s) 913
BmtI GCTAGC 1 cut(s) 5040
BmuI ACTGGG 1 cut(s) 913
BpiI GAAGAC 7 cut(s) 470, 1067, 2007, 2741, 3646, 3933, 3973
BplI GAGNNNNNCTC 4 cut(s) 626, 658, 4841, 4873
BpmI CTGGAG 3 cut(s) 30, 2928, 5138
Bpu10I CCTNAGC 2 cut(s) 2400, 3876
Bpu1102I GCTNAGC 3 cut(s) 2148, 4663, 5282
BpuEI CTTGAG 5 cut(s) 1430, 1551, 1615, 2356, 3933
BpuMI CCSGG 2 cut(s) 195, 1153
Bsa29I ATCGAT 1 cut(s) 488
BsaAI YACGTR 3 cut(s) 4109, 4892, 5129
BsaBI GATNNNNATC 2 cut(s) 3283, 4002
BsaHI GRCGYC 3 cut(s) 375, 593, 791
BsaI GGTCTC 4 cut(s) 821, 836, 2486, 3265
BsaWI WCCGGW 2 cut(s) 281, 368
BsaXI ACNNNNNCTCC 6 cut(s) 116, 134, 146, 164, 4967, 4997
Bse118I RCCGGY 1 cut(s) 4031
Bse1I ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 4663
Bse21I CCTNAGG 1 cut(s) 1071
Bse3DI GCAATG 3 cut(s) 1214, 2730, 4453
Bse8I GATNNNNATC 2 cut(s) 3283, 4002
BseBI CCWGG 6 cut(s) 79, 1539, 1710, 2106, 4270, 5156
BseCI ATCGAT 1 cut(s) 488
BseJI GATNNNNATC 2 cut(s) 3283, 4002
BseMI GCAATG 3 cut(s) 1214, 2730, 4453
BseNI ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 4663
BseRI GAGGAG 8 cut(s) 1056, 1554, 1757, 1940, 2342, 2388, 2931, 3936
BseSI GKGCMC 1 cut(s) 5134
BseYI CCCAGC 1 cut(s) 2613
BsgI GTGCAG 2 cut(s) 3576, 5109
Bsh1236I CGCG 6 cut(s) 242, 278, 335, 351, 716, 3729
Bsh1285I CGRYCG 2 cut(s) 127, 2128
BshNI GGYRCC 2 cut(s) 374, 652
BshVI ATCGAT 1 cut(s) 488
BsiEI CGRYCG 2 cut(s) 127, 2128
BsiHKAI GWGCWC 4 cut(s) 1042, 1717, 2941, 5134
BsiHKCI CYCGRG 2 cut(s) 1922, 3294
BsiSI CCGG 7 cut(s) 195, 282, 369, 625, 854, 1152, 4032
BslFI GGGAC 6 cut(s) 1517, 1643, 2492, 4226, 4969, 5283
BsmBI CGTCTC 3 cut(s) 32, 429, 600
BsmFI GGGAC 6 cut(s) 1517, 1643, 2492, 4226, 4969, 5283
BsmI GAATGC 6 cut(s) 113, 1153, 1653, 3112, 3797, 3990
Bso31I GGTCTC 4 cut(s) 821, 836, 2486, 3265
BsoBI CYCGRG 2 cut(s) 1922, 3294
Bsp1286I GDGCHC 7 cut(s) 180, 195, 1042, 1717, 2620, 2941, 5134
Bsp1720I GCTNAGC 3 cut(s) 2148, 4663, 5282
Bsp19I CCATGG 2 cut(s) 1722, 4114
Bsp68I TCGCGA 1 cut(s) 3729
BspDI ATCGAT 1 cut(s) 488
BspFNI CGCG 6 cut(s) 242, 278, 335, 351, 716, 3729
BspMI ACCTGC 1 cut(s) 354
BspOI GCTAGC 1 cut(s) 5040
BspQI GCTCTTC 2 cut(s) 2252, 3480
BspT107I GGYRCC 2 cut(s) 374, 652
BspTNI GGTCTC 4 cut(s) 821, 836, 2486, 3265
BsrDI GCAATG 3 cut(s) 1214, 2730, 4453
BsrFI RCCGGY 1 cut(s) 4031
BsrI ACTGG 6 cut(s) 908, 1637, 1782, 2496, 2769, 4663
BssAI RCCGGY 1 cut(s) 4031
BssNI GRCGYC 3 cut(s) 375, 593, 791
BssSI CACGAG 1 cut(s) 3609
BssT1I CCWWGG 5 cut(s) 1525, 1722, 3037, 4058, 4114
Bst2BI CACGAG 1 cut(s) 3609
Bst2UI CCWGG 6 cut(s) 79, 1539, 1710, 2106, 4270, 5156
Bst6I CTCTTC 8 cut(s) 649, 2252, 3480, 3632, 4070, 4527, 4582, 4778
BstACI GRCGYC 3 cut(s) 375, 593, 791
BstAPI GCANNNNNTGC 3 cut(s) 3062, 3437, 4802
BstBAI YACGTR 3 cut(s) 4109, 4892, 5129
BstDSI CCRYGG 4 cut(s) 309, 834, 1722, 4114
BstENI CCTNNNNNAGG 2 cut(s) 1752, 2324
BstFNI CGCG 6 cut(s) 242, 278, 335, 351, 716, 3729
BstH2I RGCGCY 2 cut(s) 378, 2155
BstHHI GCGC 8 cut(s) 84, 337, 351, 377, 1653, 2154, 3331, 4191
BstMCI CGRYCG 2 cut(s) 127, 2128
BstNI CCWGG 6 cut(s) 79, 1539, 1710, 2106, 4270, 5156
BstNSI RCATGY 7 cut(s) 2027, 2521, 2898, 3380, 4287, 4805, 5281
BstSCI CCNGG 8 cut(s) 77, 193, 1151, 1537, 1708, 2104, 4268, 5154
BstSFI CTRYAG 1 cut(s) 5015
BstSLI GKGCMC 1 cut(s) 5134
BstSNI TACGTA 2 cut(s) 4109, 4892
BstUI CGCG 6 cut(s) 242, 278, 335, 351, 716, 3729
BstV2I GAAGAC 7 cut(s) 470, 1067, 2007, 2741, 3646, 3933, 3973
BstX2I RGATCY 5 cut(s) 688, 1087, 1434, 1660, 4456
BstXI CCANNNNNNTGG 2 cut(s) 673, 838
BstYI RGATCY 5 cut(s) 688, 1087, 1434, 1660, 4456
Bsu15I ATCGAT 1 cut(s) 488
Bsu36I CCTNAGG 1 cut(s) 1071
BsuI GTATCC 1 cut(s) 335
BsuTUI ATCGAT 1 cut(s) 488
BtgI CCRYGG 4 cut(s) 309, 834, 1722, 4114
BtsI GCAGTG 2 cut(s) 486, 5213
BtuMI TCGCGA 1 cut(s) 3729
BveI ACCTGC 1 cut(s) 354
CaiI CAGNNNCTG 2 cut(s) 610, 3062
CfoI GCGC 8 cut(s) 84, 337, 351, 377, 1653, 2154, 3331, 4191
Cfr10I RCCGGY 1 cut(s) 4031
Cfr13I GGNCC 6 cut(s) 197, 1167, 2079, 3034, 5257, 5297
ClaI ATCGAT 1 cut(s) 488
Csp6I GTAC 6 cut(s) 226, 901, 2332, 3380, 3692, 3742
CspCI CAANNNNNGTGG 6 cut(s) 1890, 1925, 2562, 2597, 2941, 2976
CviQI GTAC 6 cut(s) 226, 901, 2332, 3380, 3692, 3742
DinI GGCGCC 1 cut(s) 376
DraI TTTAAA 1 cut(s) 3024
DraIII CACNNNGTG 1 cut(s) 4993
DrdI GACNNNNNNGTC 1 cut(s) 472
DriI GACNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 4029
Eam1104I CTCTTC 8 cut(s) 649, 2252, 3480, 3632, 4070, 4527, 4582, 4778
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 8 cut(s) 649, 2252, 3480, 3632, 4070, 4527, 4582, 4778
EciI GGCGGA 4 cut(s) 692, 872, 971, 1100
Ecl136II GAGCTC 2 cut(s) 1040, 1715
Eco105I TACGTA 2 cut(s) 4109, 4892
Eco130I CCWWGG 5 cut(s) 1525, 1722, 3037, 4058, 4114
Eco147I AGGCCT 4 cut(s) 149, 4063, 4615, 4954
Eco24I GRGCYC 5 cut(s) 180, 195, 1042, 1717, 2620
Eco31I GGTCTC 4 cut(s) 821, 836, 2486, 3265
Eco32I GATATC 4 cut(s) 547, 2358, 2800, 5052
Eco47I GGWCC 4 cut(s) 1167, 2079, 5257, 5297
Eco47III AGCGCT 1 cut(s) 2153
Eco53kI GAGCTC 2 cut(s) 1040, 1715
Eco57I CTGAAG 6 cut(s) 311, 482, 506, 1794, 1823, 2620
Eco72I CACGTG 1 cut(s) 5129
Eco81I CCTNAGG 1 cut(s) 1071
Eco88I CYCGRG 2 cut(s) 1922, 3294
EcoICRI GAGCTC 2 cut(s) 1040, 1715
EcoNI CCTNNNNNAGG 2 cut(s) 1752, 2324
EcoO109I RGGNCCY 1 cut(s) 2079
EcoRII CCWGG 6 cut(s) 77, 1537, 1708, 2104, 4268, 5154
EcoRV GATATC 4 cut(s) 547, 2358, 2800, 5052
EcoT14I CCWWGG 5 cut(s) 1525, 1722, 3037, 4058, 4114
EcoT22I ATGCAT 3 cut(s) 1954, 2025, 3922
EcoT38I GRGCYC 5 cut(s) 180, 195, 1042, 1717, 2620
EgeI GGCGCC 1 cut(s) 376
EheI GGCGCC 1 cut(s) 376
ErhI CCWWGG 5 cut(s) 1525, 1722, 3037, 4058, 4114
Esp3I CGTCTC 3 cut(s) 32, 429, 600
FalI AAGNNNNNCTT 4 cut(s) 1187, 1219, 4067, 4099
FaqI GGGAC 6 cut(s) 1517, 1643, 2492, 4226, 4969, 5283
FauI CCCGC 3 cut(s) 166, 313, 2912
FauNDI CATATG 1 cut(s) 3082
FbaI TGATCA 1 cut(s) 4258
FblI GTMKAC 1 cut(s) 1443
FriOI GRGCYC 5 cut(s) 180, 195, 1042, 1717, 2620
FspI TGCGCA 2 cut(s) 1652, 3330
GlaI GCGC 8 cut(s) 83, 336, 350, 376, 1652, 2153, 3330, 4190
GsaI CCCAGC 1 cut(s) 2617
GsuI CTGGAG 3 cut(s) 30, 2928, 5138
HaeII RGCGCY 2 cut(s) 378, 2155
HapII CCGG 7 cut(s) 195, 282, 369, 625, 854, 1152, 4032
HhaI GCGC 8 cut(s) 84, 337, 351, 377, 1653, 2154, 3331, 4191
Hin1I GRCGYC 3 cut(s) 375, 593, 791
Hin6I GCGC 8 cut(s) 82, 335, 349, 375, 1651, 2152, 3329, 4189
HinP1I GCGC 8 cut(s) 82, 335, 349, 375, 1651, 2152, 3329, 4189
HincII GTYRAC 1 cut(s) 1236
HindII GTYRAC 1 cut(s) 1236
HindIII AAGCTT 3 cut(s) 800, 1776, 2874
HpaII CCGG 7 cut(s) 195, 282, 369, 625, 854, 1152, 4032
HphI GGTGA 9 cut(s) 625, 1540, 1699, 1911, 1925, 2120, 2266, 4726, 4736
Hpy99I CGWCG 7 cut(s) 212, 242, 586, 652, 793, 2279, 5112
HpyCH4IV ACGT 6 cut(s) 1553, 4108, 4671, 4891, 4989, 5128
HpySE526I ACGT 6 cut(s) 1553, 4108, 4671, 4891, 4989, 5128
Hsp92I GRCGYC 3 cut(s) 375, 593, 791
HspAI GCGC 8 cut(s) 82, 335, 349, 375, 1651, 2152, 3329, 4189
KasI GGCGCC 1 cut(s) 374
Ksp22I TGATCA 1 cut(s) 4258
LguI GCTCTTC 2 cut(s) 2252, 3480
MaeII ACGT 6 cut(s) 1553, 4108, 4671, 4891, 4989, 5128
MfeI CAATTG 5 cut(s) 1646, 1806, 2486, 3402, 3458
MflI RGATCY 5 cut(s) 688, 1087, 1434, 1660, 4456
MhlI GDGCHC 7 cut(s) 180, 195, 1042, 1717, 2620, 2941, 5134
MluI ACGCGT 1 cut(s) 714
Mly113I GGCGCC 1 cut(s) 375
MmeI TCCRAC 6 cut(s) 55, 159, 870, 1122, 2421, 3157
Mph1103I ATGCAT 3 cut(s) 1954, 2025, 3922
MroXI GAANNNNTTC 2 cut(s) 2694, 3527
MslI CAYNNNNRTG 7 cut(s) 671, 725, 836, 2028, 2745, 3314, 3539
MspA1I CMGCKG 1 cut(s) 3059
MspI CCGG 7 cut(s) 195, 282, 369, 625, 854, 1152, 4032
MspR9I CCNGG 8 cut(s) 79, 195, 1153, 1539, 1710, 2106, 4270, 5156
MunI CAATTG 5 cut(s) 1646, 1806, 2486, 3402, 3458
Mva1269I GAATGC 6 cut(s) 113, 1153, 1653, 3112, 3797, 3990
MvaI CCWGG 6 cut(s) 79, 1539, 1710, 2106, 4270, 5156
MvnI CGCG 6 cut(s) 242, 278, 335, 351, 716, 3729
NarI GGCGCC 1 cut(s) 375
NciI CCSGG 2 cut(s) 195, 1153
NcoI CCATGG 2 cut(s) 1722, 4114
NdeI CATATG 1 cut(s) 3082
NheI GCTAGC 1 cut(s) 5036
NmeAIII GCCGAG 3 cut(s) 403, 1844, 4706
NmuCI GTSAC 7 cut(s) 481, 1546, 2498, 2755, 2836, 3397, 5273
NruI TCGCGA 1 cut(s) 3729
NsbI TGCGCA 2 cut(s) 1652, 3330
NsiI ATGCAT 3 cut(s) 1954, 2025, 3922
NspI RCATGY 7 cut(s) 2027, 2521, 2898, 3380, 4287, 4805, 5281
OliI CACNNNNGTG 1 cut(s) 836
PaeI GCATGC 1 cut(s) 2027
PceI AGGCCT 4 cut(s) 149, 4063, 4615, 4954
PciI ACATGT 1 cut(s) 2894
PciSI GCTCTTC 2 cut(s) 2252, 3480
PcsI WCGNNNNNNNCGW 4 cut(s) 216, 590, 653, 1737
PctI GAATGC 6 cut(s) 113, 1153, 1653, 3112, 3797, 3990
PdmI GAANNNNTTC 2 cut(s) 2694, 3527
PflFI GACNNNGTC 1 cut(s) 4337
PflMI CCANNNNNTGG 4 cut(s) 430, 610, 903, 4993
PluTI GGCGCC 1 cut(s) 378
PmaCI CACGTG 1 cut(s) 5129
PmlI CACGTG 1 cut(s) 5129
Ppu21I YACGTR 3 cut(s) 4109, 4892, 5129
PpuMI RGGWCCY 1 cut(s) 2079
PscI ACATGT 1 cut(s) 2894
PshBI ATTAAT 3 cut(s) 660, 3575, 4766
PsiI TTATAA 3 cut(s) 2285, 3336, 3501
Psp124BI GAGCTC 2 cut(s) 1042, 1717
Psp5II RGGWCCY 1 cut(s) 2079
Psp6I CCWGG 6 cut(s) 77, 1537, 1708, 2104, 4268, 5154
PspCI CACGTG 1 cut(s) 5129
PspFI CCCAGC 1 cut(s) 2613
PspGI CCWGG 6 cut(s) 77, 1537, 1708, 2104, 4268, 5154
PspPI GGNCC 6 cut(s) 197, 1167, 2079, 3034, 5257, 5297
PspPPI RGGWCCY 1 cut(s) 2079
PstNI CAGNNNCTG 2 cut(s) 610, 3062
PsuI RGATCY 5 cut(s) 688, 1087, 1434, 1660, 4456
PsyI GACNNNGTC 1 cut(s) 4337
PvuII CAGCTG 1 cut(s) 3059
RruI TCGCGA 1 cut(s) 3729
RsaI GTAC 6 cut(s) 227, 902, 2333, 3381, 3693, 3743
RsaNI GTAC 6 cut(s) 226, 901, 2332, 3380, 3692, 3742
RseI CAYNNNNRTG 7 cut(s) 671, 725, 836, 2028, 2745, 3314, 3539
SacI GAGCTC 2 cut(s) 1042, 1717
SapI GCTCTTC 2 cut(s) 2252, 3480
Sau96I GGNCC 6 cut(s) 197, 1167, 2079, 3034, 5257, 5297
ScaI AGTACT 3 cut(s) 227, 902, 2333
ScrFI CCNGG 8 cut(s) 79, 195, 1153, 1539, 1710, 2106, 4270, 5156
SduI GDGCHC 7 cut(s) 180, 195, 1042, 1717, 2620, 2941, 5134
SfcI CTRYAG 1 cut(s) 5015
SfoI GGCGCC 1 cut(s) 376
SinI GGWCC 4 cut(s) 1167, 2079, 5257, 5297
SmiMI CAYNNNNRTG 7 cut(s) 671, 725, 836, 2028, 2745, 3314, 3539
SmlI CTYRAG 5 cut(s) 1409, 1566, 1594, 2335, 3948
SmoI CTYRAG 5 cut(s) 1409, 1566, 1594, 2335, 3948
SnaBI TACGTA 2 cut(s) 4109, 4892
SpeI ACTAGT 1 cut(s) 2183
SphI GCATGC 1 cut(s) 2027
SseBI AGGCCT 4 cut(s) 149, 4063, 4615, 4954
SspDI GGCGCC 1 cut(s) 374
SspI AATATT 3 cut(s) 2458, 3688, 4176
SstI GAGCTC 2 cut(s) 1042, 1717
StuI AGGCCT 4 cut(s) 149, 4063, 4615, 4954
StyD4I CCNGG 8 cut(s) 77, 193, 1151, 1537, 1708, 2104, 4268, 5154
StyI CCWWGG 5 cut(s) 1525, 1722, 3037, 4058, 4114
TaiI ACGT 6 cut(s) 1556, 4111, 4674, 4894, 4992, 5131
TaqI TCGA 8 cut(s) 123, 248, 405, 488, 647, 1884, 2124, 3568
TaqII GACCGA 2 cut(s) 2142, 5285
TatI WGTACW 5 cut(s) 225, 900, 2331, 3379, 3691
TauI GCSGC 6 cut(s) 275, 278, 354, 2723, 3192, 5036
TseFI GTSAC 7 cut(s) 481, 1546, 2498, 2755, 2836, 3397, 5273
Tsp45I GTSAC 7 cut(s) 481, 1546, 2498, 2755, 2836, 3397, 5273
TspGWI ACGGA 9 cut(s) 354, 2206, 2368, 2456, 3217, 3729, 3927, 4769, 5125
Tth111I GACNNNGTC 1 cut(s) 4337
Van91I CCANNNNNTGG 4 cut(s) 430, 610, 903, 4993
VneI GTGCAC 1 cut(s) 5130
VpaK11BI GGWCC 4 cut(s) 1167, 2079, 5257, 5297
VspI ATTAAT 3 cut(s) 660, 3575, 4766
XagI CCTNNNNNAGG 2 cut(s) 1752, 2324
XapI RAATTY 2 cut(s) 169, 3515
XceI RCATGY 7 cut(s) 2027, 2521, 2898, 3380, 4287, 4805, 5281
XcmI CCANNNNNNNNNTGG 2 cut(s) 4655, 5167
XmaJI CCTAGG 1 cut(s) 4058
XmiI GTMKAC 1 cut(s) 1443
XmnI GAANNNNTTC 2 cut(s) 2694, 3527
ZrmI AGTACT 3 cut(s) 227, 902, 2333
Zsp2I ATGCAT 3 cut(s) 1954, 2025, 3922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.