MD07G1123200.v1.1

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007735
Physical Location & Seq
Forward (+)
15877749 .. 15887711
9963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1123200.v1.1.491

Sequence Viewer

Length: 5322 bp
ATGGCTTCTTCGGAAGCCGATTCCCGTCTGAGGCAGGTGTTGTCCCCAGCCCTCGATAAGATCATCAAGAACGCCTCATGGCGGAAGCACGCCAAGCTCGCCAGCGAATGCAAAGCCGTGCTCGAGCGGCTCAGCAAGTCAAAACCCGACTCGAATTCTGACCCGGACAACTCTGAGCCGGGCCCTCTCCATGACGGAGGCTCCGAAGAGTACTCTCTTGCCGACTCCGAGTCCATTCTCAGCCCCGTGATCAACGCCGCCGGCTCCGGCGTATTGAAGATCGCCGATCCGGCTGTCGATTGCATCCAGAAGCTCATCGCCCATGGCTACTTGCGCGGCGAGGCTGACGCATCCGGTGGCGCCGCGGAAGCGAAGTTGCTCACGAGATTGATCGAGTCTGTGTGCAAATGCCACGATTTGGGGGACGATCAGATGGAGCTTCTGGTGTTGAAAACGCTGTTATCGGCAGTGACATCGATGTCGCTGCGAATTCATGGCGATTGCTTGCTGCAGATGGTGAGGACTTGCTACGACATCTACTTGGGGAGTAAGAATGTCGTTAATCAGACTACGGCGAAGGCGTCGCTGATCCAAATGCTGGTGATCGTGTTCAGAAGAATGGAGGCTGACTCGTCGACGGTTCCGATTCACCCGATCGTGGTGGCTGAGCTGATGGGTCCGATTGAGAAAGCCGATGCTGACGGCTCGATGACAATGTTCGTGCAGGGGTTTATCACCAAGATTATGTCCGACATTGATGGAGTTTTGAACCCTTCGACACCTACTAAGGTGTCATTGCGGCACGACGGGGCGTTCGAGACCACCACAGTGGAGACCACGAACCCTGCCGATTTGCTGGATTCGACTGATAAGGACATGTTGGATGCCAAATACTGGGAGATTAGTATGTACAAGACGGCATTGGAGGGCCGAAAAGGCGAATTGGCGGACGGAGAATTGGAGAGAGATGAGGATTTGGAGGTTCGGATTGGGAATAAGTTGAGAAGAGATGCGTTTTTGGTGTTTAGAGCTCTGTGTAAGCTGTCTATGAAGACTCCACCAAAAGAGGCATTGGCGGATCCGGAGTTGATGAAGGGAAAGATTGTTGCTTTGGAGCTTTTGAAGATTTTGTTGGAGAATGCTGGTGCTGTCTTTAGGACCAGCGAAAGGTTTTTAGGTGCGATTAAGCAATATTTATGTCTGTCACTGTTAAAGAACAGTGCTTCAACTCTTATGATAGTTTTTCAGCTATCTTGCTCCATCTTCATTAGTCTGGTGTCAAGATTTAGAGCTGGATTGAAAGCAGAGATTGGAGTATTTTTTCCTATGATTGTTCTCAGAGTTTTGGAAAATGTTGCACAACCCAATTTTCAGCAGAAGATGATAGTACTTCGGTTTCTTGAGAAGCTCTGTGTTGATTCCCAGATCTTGGTAGACGTATTTATCAACTATGATTGTGATGTCAATTCATCGAACATATTTGAGAGGATGGTAAATGGCCTTCTTAAGACTGCCCAAGGTGTCCCTCCTGGTGTTGCCACCACATTCTTACCACCTCAGGAGGCAACTATGAAACTTGAAGCTATGAAGTGCTTAGTTGGTGTTTTGAGGTCGATTGGAGATTGGATGAATAAACAATTGCGTATTCCTGATCCTCATTCTAACAAGAAATTTGAGCCAACTGAGAATATTCCTGAACCAGGAGGTCTTCCCATGTCAAATGGGAACAGCGAGGAGCCTGTTGAAGGGTCAGACACTCATTCTGAAGCCTCCAGTGAGGCCTCTGACGCATTGACAATTGAACAACGCCGGGCTTACAAGCTGGAACTTCAGGAAGGTATATCTCTTTTCAATCGGAAACCTAAAAAAGGAATTGAGTTTCTCATTAATGCAAACAAGGTGGGTAGTTCACCTGAGGAGATAGCTGCTTTTCTCAAAAATGGATCTGGTTTGAACAAGACTTTGATTGGGGATTACCTTGGAGAAAGAGAAGACTTATCGCTAAAAGTAATGCATGCTTATGTGGATTCCTTTGAATTTCGAGGCTTGGAGTTTGATGAGGCCATCAGGACCTTTCTTCAAGACTTTAGGTTGCCTGGTGAGGCACAGAAGATCGACAGAATAATGGAAAAGTTTGCGGAACGCTATTGCAAATGTAATCAGAAGGCTTTTACTAGTGCTGACACAGCCTATGTCCTTGCTTACTCCGTGATAATGCTCAACACTGATGCTCATAACCCTATGGTGAAGAACAAGATGTCAGCTGATGATTTTATAAGAAACAATCGTGGCATAGATGATGGAAAAGATCTACCTGAGGAGTACTTGAGGTCATTGTTTGAGCGAATATCAAGAAATGAGATTAAAATGAAAGAATATGAATTGGCTCCTCAAATACAGTCGGTGAACCCAAATCGGCTTTTGGGCTTGGACAGTATCTTGAATATTGTGATCCGTAAACGGGGGGAAGGAAATCAATTGGAAACCAGTGATGATCTTATCAAGCATATGCAAGAACAATTTAAAGAAAAAGCTCGCAAATCTGAGTCAGTTTATTATGCTGCCACAGATGTGATAATTCTTAGATTCATGGTTGAGGTATGCTGGGCCCCTATGTTGGCTGCCTTCAGTGTTCCACTTGATCAAAGTGATGATGAAGTAGTTATTTCACTGTGTCTGGAAGGCTTTCGTCAGGCCATTCATGTTACTGCTGTAATGTCCATGAAGACCCATAGAGATGCTTTTGTCACTTCACTAGCAAAGTTTACTGCTCTCCACTCTCCTGCTGACATCAAGCAGAAGAATATTGATGCAATTAAGGCAATAGTGACAATTGCAGATGAAGATGGGAACTACCTCCAAGAAGCTTGGGAGCATATTTTGACATGTGTTTCTCGGTTTGAACATCTACATCTCTTGGGAGAGGGTGCTCCTCCAGATGCCACGTTCTTTGCTTTTCCTCAGAATGAGTCAGAAAAATCAAAACAGGCTAAGTCAACTATCCTTCCTGTTTTGAAAAAGAAGGGGCAAGGAAGGATGCAGTATGCAGCAGCTGCTGTGTTGCGAGGTTCATATGATAGTGCTGGTATTGGTGGGAATGCTTCTGGGGCGGTCACATCAGAACAGATGAACAATTTGGTTTCTAATTTAAACATGCTAGAACAAGTTGGTGAAATGAGCCGCATATTCACACGGAGTGAGAAGCTAAACAGTGAGGCAATTGTAGATTTTGTTAAGGCACTTTGCAAGGTCTCTATGGAAGAATTGCGATCGGCATCTGATCCTCGAGTTTTCAGCCTTACAAAGATTGTTGAGATTGCGCATTATAACATGAATCGCATCAGGCTTGTGTGGTCTAGCATCTGGCATGTGCTCTCTAATTTCTTCGTGACAATTGGCTGTTCTGAAAACCTGTCAATTGCCATTTTTGCTATGGATTCTCTACGCCAGTTGTCAATGAAATTTTTGGACCGAGAAGAGTTGGCTAATTATAATTTTCAGAATGAGTTTATGAAACCTTTTGTCATTGTTATGCGTAAAAGCAGTGCAGTTGAAATACGTGAATTGATCATCAGATGTGTTTCACAAATGGTATTTTCTCGTGTCAACAATGTCAAATCTGGATGGAAGAGCATGTTCATGGTCTTCACTACGGCAGCTTATGACGACCACAAAAACATTGTACTCTTGGCCTTTGAAATAATTGAGAAGATTATTCGAGACTATTTTCCGTACATTACTGAGACTGAAACCACTACCTTTACCGACTGCGTGAATTGCCTAATTGCATTTACCAATAGTAGATTCAACAGAGACATTAGCCTCAATGCTATTGCTTTTCTTCGATTTTGTGCTACAAAACTTGCAGATGGAGGTCTTGGTTCCACATCAAAAAATAAGGACAAGGAGGCTTCTGTAAAAATCTCTCCATCTTCACCTCAAGCCTGGAAAGGTGGAAAACAAGAAAATGGTGAAATACCAGATAAGGACGACCATATCTATTGCTGGTTCCCCTTATTGGCTGGTTTATCCGAGCTTAGCTTTGATCCTAGGCCTGAAATCAGGAGGAGTGCCTTACAAGTACTGTTTGAAACTTTACGTAACCATGGTCACCTTTTCTCACTACCTTTGTGGGAAAGGGTGTTTGAATCAGTGCTATTTCCAATATTTGATTATGTGCGGCATGCTATTGATCCTTCTGGGGAAGGTTCACCAGGGCAAGGGACTGATGGTGATGTGGACGACCTTGACCAAGATGCCTGGCTCTATGAGACATGCACATTGGCACTTCAATTAGTTGTTGATCTCTTTGTCAAATTTTATACCACAGTCAATCCGCTATTGAAGAAAGTTTTGGTGCTTTTAGTCAGTTTTATTGGACGACCACACCAAAGCTTAGCTGGTATAGGTATAGCTGCATTTGTGCGGTTGATGAGCAATGCAGGGGATCTATTTTCTGATGAAAAGTGGCTACAAGTGGTTTCATCATTAAAAGAAGCAGCCAATTCAGCGCTTCCTGATTTCTCTTTCATTGTTAGTGGAGATAGTATCATTAGAAGCCACGAACATGCTTTGAGCAGAGAAGAGAATGGAGAGGCCACTGTGTCTGGCAGGAGGGATGAGGATTCAGAGAGATTGAGGACAAACCACCTGTATGCTGGAATATCTGACGTCAAGTGCCGAGCTGCTGTTCAACTTTTACTGATTCAGGCGGTGATGGAGATTTACACCATGTACCGGTCTCACCTTTCTGCGAGGAACACGTTAGTCCTTTTTGATGCTTTGCATGATGTTGCATCTCACGCTCACAAGATTAACACTGATACCACTTTACGAGCAAGGTTACAAGAATTTGGCTCCGTGACCCAAATGCAAGACCCTCCACTGTTGCGCATCGAGAACGAATCTTACCAAATATGCCTTACATTGTTACAGAACATTATAGAGGATACACCGACAGGTTACAATGAGGCGGAGGTGGAGTCCTACATCGTGGACCTCTGTAGAGAGGTTTTACAGTTCTATATTGAAGCTGCTAGCTCTGGAAAGGTATCTGAATCATCCAAGGGGCAGCAGCACCATTGGCTAATTCCTCTAGGTTCTGGGGGACGAAGAGAGTTGGCTCAACGTGCCCCGCTTATTGTGGCAACTCTCCAGACTATCTGTAGTTTGGGAGAGACCTCATTTGAAAACAACCTGCCTCAGTTCTTCCCCCTTCTTTCCAGCTTGATAAGTTGTGAACATGGTTCAAATGAGGTCCAGATTGCCCTCGGTGACATGCTCAGCTCATCCGTGGGGCCTGTGTTGCTTCGGTCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1774

Amino Acids

197.52

Weight (kDa)

5.48

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 6 - 210 1.6e-37 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 335 - 493 2.6e-47 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 607 - 788 4.6e-78 Sec7 domain
Sec7-like_HDS PF09324 1154 - 1236 9.8e-30 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1334 - 1396 7.6e-11 C-terminal region of Mon2 protein
BIG2_C PF20252 1563 - 1762 1.2e-42 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2276, 3327, 3492
AarI CACCTGC 1 cut(s) 25
AasI GACNNNNNNGTC 1 cut(s) 478
AatII GACGTC 1 cut(s) 4674
Acc16I TGCGCA 2 cut(s) 3321, 4892
Acc36I ACCTGC 2 cut(s) 25, 5205
AccB1I GGYRCC 1 cut(s) 359
AccB7I CCANNNNNTGG 4 cut(s) 418, 598, 894, 1429
AccBSI CCGCTC 1 cut(s) 127
AccI GTMKAC 2 cut(s) 635, 1434
AccII CGCG 2 cut(s) 336, 365
AccIII TCCGGA 1 cut(s) 1081
AcsI RAATTY 7 cut(s) 154, 489, 1670, 2036, 3461, 4316, 4850
AcuI CTGAAG 3 cut(s) 1785, 1814, 2611
AcyI GRCGYC 3 cut(s) 360, 581, 4671
AdeI CACNNNGTG 1 cut(s) 3197
AfaI GTAC 8 cut(s) 212, 911, 1389, 2324, 3684, 3734, 4083, 4736
AfeI AGCGCT 1 cut(s) 4512
AflII CTTAAG 1 cut(s) 1505
AflIII ACRYGT 3 cut(s) 876, 2885, 4761
AgeI ACCGGT 1 cut(s) 4737
AhdI GACNNNNNGTC 1 cut(s) 229
AhlI ACTAGT 1 cut(s) 2174
AjnI CCWGG 6 cut(s) 1528, 1699, 2095, 3944, 4213, 4259
AjuI GAANNNNNNNTTGG 6 cut(s) 1115, 1147, 3788, 3820, 4337, 4369
AleI CACNNNNGTG 2 cut(s) 827, 2570
Alw21I GWGCWC 4 cut(s) 123, 1033, 2932, 3375
Alw26I GTCTC 9 cut(s) 812, 827, 3256, 3714, 3737, 3807, 4265, 4746, 5171
AlwNI CAGNNNCTG 2 cut(s) 3053, 3056
Ama87I CYCGRG 2 cut(s) 122, 3285
Aor13HI TCCGGA 1 cut(s) 1081
Aor51HI AGCGCT 1 cut(s) 4512
ApaI GGGCCC 2 cut(s) 185, 2611
ApoI RAATTY 7 cut(s) 154, 489, 1670, 2036, 3461, 4316, 4850
ArsI GACNNNNNNTTYG 2 cut(s) 797, 829
AseI ATTAAT 1 cut(s) 1887
AsiGI ACCGGT 1 cut(s) 4737
Asp700I GAANNNNTTC 1 cut(s) 2685
AspA2I CCTAGG 1 cut(s) 4049
AspLEI GCGC 5 cut(s) 336, 362, 3322, 4513, 4893
AsuC2I CCSGG 3 cut(s) 164, 180, 1811
AsuNHI GCTAGC 1 cut(s) 5036
AvaI CYCGRG 2 cut(s) 122, 3285
AvaII GGWCC 6 cut(s) 677, 1158, 2070, 3469, 4996, 5257
AvrII CCTAGG 1 cut(s) 4049
AxyI CCTNAGG 3 cut(s) 1557, 1914, 2316
BaeGI GKGCMC 3 cut(s) 185, 2611, 5134
BamHI GGATCC 1 cut(s) 1078
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 3 cut(s) 185, 1033, 2611
BarI GAAGNNNNNNTAC 2 cut(s) 1485, 1517
BauI CACGAG 2 cut(s) 382, 3600
BbsI GAAGAC 5 cut(s) 1058, 1700, 1998, 2732, 3637
Bbv12I GWGCWC 4 cut(s) 123, 1033, 2932, 3375
BceAI ACGGC 5 cut(s) 101, 588, 718, 933, 3669
BcgI CGANNNNNNTGC 4 cut(s) 456, 466, 490, 500
BciT130I CCWGG 6 cut(s) 1530, 1701, 2097, 3946, 4215, 4261
BciVI GTATCC 1 cut(s) 4942
BclI TGATCA 3 cut(s) 249, 2641, 3567
BcnI CCSGG 3 cut(s) 164, 180, 1811
BcoDI GTCTC 9 cut(s) 812, 827, 3256, 3714, 3737, 3807, 4265, 4746, 5171
BcuI ACTAGT 1 cut(s) 2174
BfaI CTAG 7 cut(s) 2175, 2756, 3158, 3357, 4050, 5037, 5096
BfmI CTRYAG 3 cut(s) 509, 5002, 5164
BfoI RGCGCY 2 cut(s) 363, 4514
BfrI CTTAAG 1 cut(s) 1505
BfuAI ACCTGC 2 cut(s) 25, 5205
BfuI GTATCC 1 cut(s) 4942
BglI GCCNNNNNGGC 2 cut(s) 290, 936
BglII AGATCT 2 cut(s) 1425, 2308
BlnI CCTAGG 1 cut(s) 4049
BlpI GCTNAGC 5 cut(s) 131, 666, 4037, 4396, 5282
BmcAI AGTACT 4 cut(s) 212, 1389, 2324, 4083
Bme1390I CCNGG 9 cut(s) 164, 180, 1530, 1701, 1811, 2097, 3946, 4215, 4261
Bme18I GGWCC 6 cut(s) 677, 1158, 2070, 3469, 4996, 5257
BmeRI GACNNNNNGTC 1 cut(s) 229
BmeT110I CYCGRG 2 cut(s) 122, 3285
BmrFI CCNGG 9 cut(s) 164, 180, 1530, 1701, 1811, 2097, 3946, 4215, 4261
BmrI ACTGGG 1 cut(s) 904
BmtI GCTAGC 1 cut(s) 5040
BmuI ACTGGG 1 cut(s) 904
BpiI GAAGAC 5 cut(s) 1058, 1700, 1998, 2732, 3637
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 3 cut(s) 1756, 2919, 5138
Bpu1102I GCTNAGC 5 cut(s) 131, 666, 4037, 4396, 5282
BpuEI CTTGAG 3 cut(s) 1421, 2347, 3924
BpuMI CCSGG 3 cut(s) 164, 180, 1811
Bsa29I ATCGAT 1 cut(s) 476
BsaAI YACGTR 2 cut(s) 3560, 4100
BsaBI GATNNNNATC 1 cut(s) 3274
BsaHI GRCGYC 3 cut(s) 360, 581, 4671
BsaI GGTCTC 5 cut(s) 812, 827, 3256, 4746, 5171
BsaWI WCCGGW 3 cut(s) 353, 1081, 4737
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 4967, 4997
Bse118I RCCGGY 2 cut(s) 260, 4737
Bse1I ACTGG 4 cut(s) 899, 1773, 2487, 3448
Bse21I CCTNAGG 3 cut(s) 1557, 1914, 2316
Bse3DI GCAATG 2 cut(s) 794, 4444
Bse8I GATNNNNATC 1 cut(s) 3274
BseAI TCCGGA 1 cut(s) 1081
BseBI CCWGG 6 cut(s) 1530, 1701, 2097, 3946, 4215, 4261
BseCI ATCGAT 1 cut(s) 476
BseJI GATNNNNATC 1 cut(s) 3274
BseMI GCAATG 2 cut(s) 794, 4444
BseNI ACTGG 4 cut(s) 899, 1773, 2487, 3448
BseRI GAGGAG 6 cut(s) 1748, 1931, 2333, 2379, 2922, 4081
BseSI GKGCMC 3 cut(s) 185, 2611, 5134
BseYI CCCAGC 2 cut(s) 46, 2604
BsgI GTGCAG 2 cut(s) 743, 3567
Bsh1236I CGCG 2 cut(s) 336, 365
Bsh1285I CGRYCG 2 cut(s) 657, 3272
BshNI GGYRCC 1 cut(s) 359
BshTI ACCGGT 1 cut(s) 4737
BshVI ATCGAT 1 cut(s) 476
BsiEI CGRYCG 2 cut(s) 657, 3272
BsiHKAI GWGCWC 4 cut(s) 123, 1033, 2932, 3375
BsiHKCI CYCGRG 2 cut(s) 122, 3285
BsiSI CCGG 9 cut(s) 164, 179, 261, 267, 290, 354, 1082, 1810, 4738
BslFI GGGAC 5 cut(s) 28, 437, 1508, 4237, 5121
BsmAI GTCTC 9 cut(s) 812, 827, 3256, 3714, 3737, 3807, 4265, 4746, 5171
BsmFI GGGAC 5 cut(s) 28, 437, 1508, 4237, 5121
BsmI GAATGC 3 cut(s) 113, 1144, 3103
Bso31I GGTCTC 5 cut(s) 812, 827, 3256, 4746, 5171
BsoBI CYCGRG 2 cut(s) 122, 3285
Bsp120I GGGCCC 2 cut(s) 181, 2607
Bsp1286I GDGCHC 7 cut(s) 123, 185, 1033, 2611, 2932, 3375, 5134
Bsp13I TCCGGA 1 cut(s) 1081
Bsp1407I TGTACA 1 cut(s) 909
Bsp1720I GCTNAGC 5 cut(s) 131, 666, 4037, 4396, 5282
Bsp19I CCATGG 2 cut(s) 322, 4105
BspDI ATCGAT 1 cut(s) 476
BspEI TCCGGA 1 cut(s) 1081
BspFNI CGCG 2 cut(s) 336, 365
BspMAI CTGCAG 1 cut(s) 513
BspMI ACCTGC 2 cut(s) 25, 5205
BspOI GCTAGC 1 cut(s) 5040
BspQI GCTCTTC 1 cut(s) 3623
BspT107I GGYRCC 1 cut(s) 359
BspTI CTTAAG 1 cut(s) 1505
BspTNI GGTCTC 5 cut(s) 812, 827, 3256, 4746, 5171
BsrBI CCGCTC 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 794, 4444
BsrFI RCCGGY 2 cut(s) 260, 4737
BsrGI TGTACA 1 cut(s) 909
BsrI ACTGG 4 cut(s) 899, 1773, 2487, 3448
BssAI RCCGGY 2 cut(s) 260, 4737
BssNI GRCGYC 3 cut(s) 360, 581, 4671
BssSI CACGAG 2 cut(s) 382, 3600
BssT1I CCWWGG 6 cut(s) 322, 1516, 1978, 4049, 4105, 5064
Bst2BI CACGAG 2 cut(s) 382, 3600
Bst2UI CCWGG 6 cut(s) 1530, 1701, 2097, 3946, 4215, 4261
Bst6I CTCTTC 6 cut(s) 201, 1000, 3471, 3623, 4578, 5107
BstACI GRCGYC 3 cut(s) 360, 581, 4671
BstAFI CTTAAG 1 cut(s) 1505
BstAPI GCANNNNNTGC 1 cut(s) 3053
BstAUI TGTACA 1 cut(s) 909
BstBAI YACGTR 2 cut(s) 3560, 4100
BstC8I GCNNGC 9 cut(s) 90, 99, 103, 262, 506, 2016, 2536, 4185, 5038
BstDSI CCRYGG 4 cut(s) 322, 363, 4105, 5292
BstEII GGTNACC 1 cut(s) 4109
BstENI CCTNNNNNAGG 3 cut(s) 1698, 1743, 1866
BstFNI CGCG 2 cut(s) 336, 365
BstH2I RGCGCY 2 cut(s) 363, 4514
BstHHI GCGC 5 cut(s) 336, 362, 3322, 4513, 4893
BstMAI GTCTC 9 cut(s) 812, 827, 3256, 3714, 3737, 3807, 4265, 4746, 5171
BstMCI CGRYCG 2 cut(s) 657, 3272
BstNI CCWGG 6 cut(s) 1530, 1701, 2097, 3946, 4215, 4261
BstPI GGTNACC 1 cut(s) 4109
BstSCI CCNGG 9 cut(s) 162, 178, 1528, 1699, 1809, 2095, 3944, 4213, 4259
BstSFI CTRYAG 3 cut(s) 509, 5002, 5164
BstSLI GKGCMC 3 cut(s) 185, 2611, 5134
BstSNI TACGTA 1 cut(s) 4100
BstUI CGCG 2 cut(s) 336, 365
BstV2I GAAGAC 5 cut(s) 1058, 1700, 1998, 2732, 3637
BstX2I RGATCY 5 cut(s) 1078, 1425, 1943, 2308, 4447
BstXI CCANNNNNNTGG 1 cut(s) 829
BstYI RGATCY 5 cut(s) 1078, 1425, 1943, 2308, 4447
Bsu15I ATCGAT 1 cut(s) 476
Bsu36I CCTNAGG 3 cut(s) 1557, 1914, 2316
BsuI GTATCC 1 cut(s) 4942
BsuTUI ATCGAT 1 cut(s) 476
BtgI CCRYGG 4 cut(s) 322, 363, 4105, 5292
BtgZI GCGATG 1 cut(s) 301
BtsI GCAGTG 2 cut(s) 474, 3550
BveI ACCTGC 2 cut(s) 25, 5205
Cac8I GCNNGC 9 cut(s) 90, 99, 103, 262, 506, 2016, 2536, 4185, 5038
CaiI CAGNNNCTG 2 cut(s) 3053, 3056
CfoI GCGC 5 cut(s) 336, 362, 3322, 4513, 4893
Cfr10I RCCGGY 2 cut(s) 260, 4737
Cfr42I CCGCGG 1 cut(s) 366
ClaI ATCGAT 1 cut(s) 476
CseI GACGC 3 cut(s) 356, 570, 1796
Csp6I GTAC 8 cut(s) 211, 910, 1388, 2323, 3683, 3733, 4082, 4735
CspAI ACCGGT 1 cut(s) 4737
CspCI CAANNNNNGTGG 4 cut(s) 1881, 1916, 2932, 2967
CviQI GTAC 8 cut(s) 211, 910, 1388, 2323, 3683, 3733, 4082, 4735
DinI GGCGCC 1 cut(s) 361
DraI TTTAAA 2 cut(s) 2524, 3150
DraIII CACNNNGTG 1 cut(s) 3197
DrdI GACNNNNNNGTC 1 cut(s) 478
DriI GACNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 478
Eam1104I CTCTTC 6 cut(s) 201, 1000, 3471, 3623, 4578, 5107
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 6 cut(s) 201, 1000, 3471, 3623, 4578, 5107
EciI GGCGGA 4 cut(s) 97, 962, 1091, 4988
Ecl136II GAGCTC 1 cut(s) 1031
Eco105I TACGTA 1 cut(s) 4100
Eco130I CCWWGG 6 cut(s) 322, 1516, 1978, 4049, 4105, 5064
Eco147I AGGCCT 2 cut(s) 1781, 4054
Eco24I GRGCYC 3 cut(s) 185, 1033, 2611
Eco31I GGTCTC 5 cut(s) 812, 827, 3256, 4746, 5171
Eco47I GGWCC 6 cut(s) 677, 1158, 2070, 3469, 4996, 5257
Eco47III AGCGCT 1 cut(s) 4512
Eco53kI GAGCTC 1 cut(s) 1031
Eco57I CTGAAG 3 cut(s) 1785, 1814, 2611
Eco81I CCTNAGG 3 cut(s) 1557, 1914, 2316
Eco88I CYCGRG 2 cut(s) 122, 3285
Eco91I GGTNACC 1 cut(s) 4109
EcoICRI GAGCTC 1 cut(s) 1031
EcoNI CCTNNNNNAGG 3 cut(s) 1698, 1743, 1866
EcoO109I RGGNCCY 4 cut(s) 182, 2070, 2608, 5297
EcoO65I GGTNACC 1 cut(s) 4109
EcoRI GAATTC 2 cut(s) 154, 489
EcoRII CCWGG 6 cut(s) 1528, 1699, 2095, 3944, 4213, 4259
EcoT14I CCWWGG 6 cut(s) 322, 1516, 1978, 4049, 4105, 5064
EcoT22I ATGCAT 1 cut(s) 2016
EcoT38I GRGCYC 3 cut(s) 185, 1033, 2611
EgeI GGCGCC 1 cut(s) 361
EheI GGCGCC 1 cut(s) 361
ErhI CCWWGG 6 cut(s) 322, 1516, 1978, 4049, 4105, 5064
FaqI GGGAC 5 cut(s) 28, 437, 1508, 4237, 5121
FauI CCCGC 1 cut(s) 5142
FauNDI CATATG 2 cut(s) 2508, 3073
FbaI TGATCA 3 cut(s) 249, 2641, 3567
FblI GTMKAC 2 cut(s) 635, 1434
FriOI GRGCYC 3 cut(s) 185, 1033, 2611
FspBI CTAG 7 cut(s) 2175, 2756, 3158, 3357, 4050, 5037, 5096
FspI TGCGCA 2 cut(s) 3321, 4892
GlaI GCGC 5 cut(s) 335, 361, 3321, 4512, 4892
GsaI CCCAGC 2 cut(s) 50, 2608
GsuI CTGGAG 3 cut(s) 1756, 2919, 5138
HaeII RGCGCY 2 cut(s) 363, 4514
HapII CCGG 9 cut(s) 164, 179, 261, 267, 290, 354, 1082, 1810, 4738
HgaI GACGC 3 cut(s) 356, 570, 1796
HhaI GCGC 5 cut(s) 336, 362, 3322, 4513, 4893
Hin1I GRCGYC 3 cut(s) 360, 581, 4671
Hin6I GCGC 5 cut(s) 334, 360, 3320, 4511, 4891
HinP1I GCGC 5 cut(s) 334, 360, 3320, 4511, 4891
HincII GTYRAC 3 cut(s) 636, 2997, 3607
HindII GTYRAC 3 cut(s) 636, 2997, 3607
HindIII AAGCTT 2 cut(s) 2865, 4393
HpaII CCGG 9 cut(s) 164, 179, 261, 267, 290, 354, 1082, 1810, 4738
Hpy99I CGWCG 4 cut(s) 586, 637, 640, 809
HpyCH4IV ACGT 7 cut(s) 1437, 2945, 3559, 4099, 4671, 4763, 5128
HpySE526I ACGT 7 cut(s) 1437, 2945, 3559, 4099, 4671, 4763, 5128
Hsp92I GRCGYC 3 cut(s) 360, 581, 4671
HspAI GCGC 5 cut(s) 334, 360, 3320, 4511, 4891
KasI GGCGCC 1 cut(s) 359
Kpn2I TCCGGA 1 cut(s) 1081
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Ksp22I TGATCA 3 cut(s) 249, 2641, 3567
KspI CCGCGG 1 cut(s) 366
LguI GCTCTTC 1 cut(s) 3623
MaeI CTAG 7 cut(s) 2175, 2756, 3158, 3357, 4050, 5037, 5096
MaeII ACGT 7 cut(s) 1437, 2945, 3559, 4099, 4671, 4763, 5128
MbiI CCGCTC 1 cut(s) 127
MfeI CAATTG 7 cut(s) 1637, 1797, 2477, 2832, 3219, 3393, 3417
MflI RGATCY 5 cut(s) 1078, 1425, 1943, 2308, 4447
MhlI GDGCHC 7 cut(s) 123, 185, 1033, 2611, 2932, 3375, 5134
Mly113I GGCGCC 1 cut(s) 360
MlyI GAGTC 9 cut(s) 143, 218, 239, 404, 623, 1048, 2555, 2978, 4991
MmeI TCCRAC 3 cut(s) 774, 861, 1113
Mph1103I ATGCAT 1 cut(s) 2016
MroI TCCGGA 1 cut(s) 1081
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 2685
MslI CAYNNNNRTG 8 cut(s) 827, 2019, 2570, 2736, 3530, 3638, 4566, 4653
MspA1I CMGCKG 3 cut(s) 365, 2264, 3053
MspCI CTTAAG 1 cut(s) 1505
MspI CCGG 9 cut(s) 164, 179, 261, 267, 290, 354, 1082, 1810, 4738
MspR9I CCNGG 9 cut(s) 164, 180, 1530, 1701, 1811, 2097, 3946, 4215, 4261
MunI CAATTG 7 cut(s) 1637, 1797, 2477, 2832, 3219, 3393, 3417
Mva1269I GAATGC 3 cut(s) 113, 1144, 3103
MvaI CCWGG 6 cut(s) 1530, 1701, 2097, 3946, 4215, 4261
MvnI CGCG 2 cut(s) 336, 365
NaeI GCCGGC 1 cut(s) 262
NarI GGCGCC 1 cut(s) 360
NciI CCSGG 3 cut(s) 164, 180, 1811
NcoI CCATGG 2 cut(s) 322, 4105
NdeI CATATG 2 cut(s) 2508, 3073
NgoMIV GCCGGC 1 cut(s) 260
NheI GCTAGC 1 cut(s) 5036
NmeAIII GCCGAG 1 cut(s) 4706
NmuCI GTSAC 9 cut(s) 469, 1203, 2746, 2827, 3112, 3388, 4109, 4861, 5273
NsbI TGCGCA 2 cut(s) 3321, 4892
NsiI ATGCAT 1 cut(s) 2016
OliI CACNNNNGTG 2 cut(s) 827, 2570
PaeI GCATGC 2 cut(s) 2018, 4187
PaeR7I CTCGAG 2 cut(s) 122, 3285
PaqCI CACCTGC 1 cut(s) 25
PceI AGGCCT 2 cut(s) 1781, 4054
PciI ACATGT 2 cut(s) 876, 2885
PciSI GCTCTTC 1 cut(s) 3623
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 578, 641, 813
PctI GAATGC 3 cut(s) 113, 1144, 3103
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 2685
PflMI CCANNNNNTGG 4 cut(s) 418, 598, 894, 1429
PinAI ACCGGT 1 cut(s) 4737
Ple19I CGATCG 2 cut(s) 657, 3272
PleI GAGTC 9 cut(s) 143, 218, 238, 403, 623, 1048, 2554, 2977, 4990
PluTI GGCGCC 1 cut(s) 363
PpsI GAGTC 9 cut(s) 143, 218, 238, 403, 623, 1048, 2554, 2977, 4990
Ppu21I YACGTR 2 cut(s) 3560, 4100
PpuMI RGGWCCY 1 cut(s) 2070
PscI ACATGT 2 cut(s) 876, 2885
PshBI ATTAAT 1 cut(s) 1887
PsiI TTATAA 3 cut(s) 2276, 3327, 3492
Psp124BI GAGCTC 1 cut(s) 1033
Psp5II RGGWCCY 1 cut(s) 2070
Psp6I CCWGG 6 cut(s) 1528, 1699, 2095, 3944, 4213, 4259
PspEI GGTNACC 1 cut(s) 4109
PspFI CCCAGC 2 cut(s) 46, 2604
PspGI CCWGG 6 cut(s) 1528, 1699, 2095, 3944, 4213, 4259
PspOMI GGGCCC 2 cut(s) 181, 2607
PspPPI RGGWCCY 1 cut(s) 2070
PspXI VCTCGAGB 2 cut(s) 122, 3285
PsrI GAACNNNNNNTAC 2 cut(s) 4892, 4924
PstI CTGCAG 1 cut(s) 513
PstNI CAGNNNCTG 2 cut(s) 3053, 3056
PsuI RGATCY 5 cut(s) 1078, 1425, 1943, 2308, 4447
PvuI CGATCG 2 cut(s) 657, 3272
PvuII CAGCTG 2 cut(s) 2264, 3053
RsaI GTAC 8 cut(s) 212, 911, 1389, 2324, 3684, 3734, 4083, 4736
RsaNI GTAC 8 cut(s) 211, 910, 1388, 2323, 3683, 3733, 4082, 4735
RseI CAYNNNNRTG 8 cut(s) 827, 2019, 2570, 2736, 3530, 3638, 4566, 4653
SacI GAGCTC 1 cut(s) 1033
SacII CCGCGG 1 cut(s) 366
SalI GTCGAC 1 cut(s) 634
SapI GCTCTTC 1 cut(s) 3623
ScaI AGTACT 4 cut(s) 212, 1389, 2324, 4083
SchI GAGTC 9 cut(s) 143, 218, 239, 404, 623, 1048, 2555, 2978, 4991
ScrFI CCNGG 9 cut(s) 164, 180, 1530, 1701, 1811, 2097, 3946, 4215, 4261
SduI GDGCHC 7 cut(s) 123, 185, 1033, 2611, 2932, 3375, 5134
SfcI CTRYAG 3 cut(s) 509, 5002, 5164
SfoI GGCGCC 1 cut(s) 361
Sfr274I CTCGAG 2 cut(s) 122, 3285
Sfr303I CCGCGG 1 cut(s) 366
SgrBI CCGCGG 1 cut(s) 366
SgrDI CGTCGACG 1 cut(s) 634
SinI GGWCC 6 cut(s) 677, 1158, 2070, 3469, 4996, 5257
SlaI CTCGAG 2 cut(s) 122, 3285
SmiMI CAYNNNNRTG 8 cut(s) 827, 2019, 2570, 2736, 3530, 3638, 4566, 4653
SmlI CTYRAG 6 cut(s) 122, 1400, 1505, 2326, 3285, 3939
SmoI CTYRAG 6 cut(s) 122, 1400, 1505, 2326, 3285, 3939
SnaBI TACGTA 1 cut(s) 4100
SpeI ACTAGT 1 cut(s) 2174
SphI GCATGC 2 cut(s) 2018, 4187
SseBI AGGCCT 2 cut(s) 1781, 4054
SspDI GGCGCC 1 cut(s) 359
SspI AATATT 5 cut(s) 1193, 1690, 2446, 2806, 4167
SspMI CTAG 7 cut(s) 2175, 2756, 3158, 3357, 4050, 5037, 5096
SstI GAGCTC 1 cut(s) 1033
StuI AGGCCT 2 cut(s) 1781, 4054
StyD4I CCNGG 9 cut(s) 162, 178, 1528, 1699, 1809, 2095, 3944, 4213, 4259
StyI CCWWGG 6 cut(s) 322, 1516, 1978, 4049, 4105, 5064
TaiI ACGT 7 cut(s) 1440, 2948, 3562, 4102, 4674, 4766, 5131
TaqII GACCGA 2 cut(s) 3486, 5301
TatI WGTACW 6 cut(s) 210, 909, 1387, 2322, 3682, 4081
TauI GCSGC 7 cut(s) 130, 260, 339, 365, 802, 3183, 4183
TseFI GTSAC 9 cut(s) 469, 1203, 2746, 2827, 3112, 3388, 4109, 4861, 5273
Tsp45I GTSAC 9 cut(s) 469, 1203, 2746, 2827, 3112, 3388, 4109, 4861, 5273
TspGWI ACGGA 8 cut(s) 210, 966, 2197, 2444, 3208, 3720, 4849, 5281
Van91I CCANNNNNTGG 4 cut(s) 418, 598, 894, 1429
Vha464I CTTAAG 1 cut(s) 1505
VpaK11BI GGWCC 6 cut(s) 677, 1158, 2070, 3469, 4996, 5257
VspI ATTAAT 1 cut(s) 1887
XagI CCTNNNNNAGG 3 cut(s) 1698, 1743, 1866
XapI RAATTY 7 cut(s) 154, 489, 1670, 2036, 3461, 4316, 4850
XcmI CCANNNNNNNNNTGG 3 cut(s) 3430, 4397, 4655
XhoI CTCGAG 2 cut(s) 122, 3285
XmaJI CCTAGG 1 cut(s) 4049
XmiI GTMKAC 2 cut(s) 635, 1434
XmnI GAANNNNTTC 1 cut(s) 2685
XspI CTAG 7 cut(s) 2175, 2756, 3158, 3357, 4050, 5037, 5096
ZraI GACGTC 1 cut(s) 4672
ZrmI AGTACT 4 cut(s) 212, 1389, 2324, 4083
Zsp2I ATGCAT 1 cut(s) 2016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.