pycom07g11180

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007735
Physical Location & Seq
Forward (+)
11654965 .. 11657961
2997 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g11180.4

Sequence Viewer

Length: 1728 bp
ATGGAGCTTTTGGTGTTGAAAACGCTGTTATCGGCAGTGACATCGATATCGCTGCGAATTCACGGCGATTGCTTGCTGCAGATAGTGAGGACTTGCTACGACATCTACTTGGGGAGTAAGAATGTCGTTAATCAGACTACGGCGAAGGCGTCGCTGATCCAGATGCTGGTGATCGTGTTCAGAAGAATGGAGGCTGACTCGTCGACGGTTCCAATTCACCCAATCGTGGTGGCTGAGCTGATGGATCCGGTTGAGAAAGCCGATGCTGATGGCTCGATGACAATGTTCGTGCAGGGGTTTATCACCAAGATTATGTCCGACATTGATGGAGTTTTGAACCCTTCGACACCTACTAAGGTGTCATTGCGGCACGACGGGGCGTTTGAGACCACCACAGTGGAGACCACGAACCCTGCCGATTTGCTGGATTCGACTGATAAGGACATGTTGGATGCCAAATACTGGGAGATTAGTATGTACAAGACGGCATTGGAGGGCCGAAAAGGCGAATTGGCGGACGGAGAATTGGAGAGAGATGAGGATTTGGAGGTTCAGATTGGGAATAAGTTGAGAAGAGATGTGTTGTTGGTGTTGAGAGCTCTGTGTAAGCTGTCTATGAAGACTCCACCAAAAGAGGCATTGGCGGATCCGGAGTTGATGAAGGGAAAGATTGTTGCTTTGGAGCTTTTGAAGATTTTGTTGGAGAATGCCGGTGCTGTCTTTAGGACCAGCGAAAGGTTTTTAGGTGCGATTAAGCAATATTTATGTCTGTCACTGTTAAAGAACAGTGCTTCAACTCTTATGATAGTTTTTCAGCTATCTTGCTCCATCTTCATTAGTCTGGTGTCAAGATTTAGAGCTGGATTGAAAGCAGAGATTGGAGTATTTTTTCCTATGATTGTTCTCAGAGTTTTGGAAAATGTCGCACAACCCAATTTTCAGCAGAAGATGATAGTACTTCGGTTTCTTGAGAAGCTCTGTGTTGATTCCCAGATCTTGGTTGACATATTTATCAACTATGATTGTGATGTCAATTCATCGAACATATTTGAGAGGATGGTAAATGGCCTTCTTAAAACTGCACAAGGTGTCCCTCCTGGTGTTGCCACCACATTATTACCACCTCAGGAGGCAACTATGAAACTTGAAGCTATGAAGTGCTTAGTTGGTGTTTTGAGGTCGATCGGAGATTGGATGAACAAACAATTGCGTATTCCAGATCCTCATTCTAACAAGAGATTTGAGCCAACTGAGAATAATCCTGAACCAGGAGGTCTTCCCATGGCAAATGGGAACAGCGAGGAGCCTGTTGAAGGGTCAGACACTCATTCTGAAGCCTCCAGTGAGGCCTCTGATGCTTTGACAATTGAACAACGCCGGGCTTACAAGCTGGAACTTCAGGAAGGTATATCTCTTTTCAATCGGAAACCTAAAAAAGGAATTGAGTTTCTCATTAATGCAAACAAGGTGGGTAGTTCACCTGAGGAGATAGCTGCTTTTCTCAAAAATGGATCTGGTTTGAACAAGACTTTGATTGGGGATTACCTTGGAGAAAGAGAAGACTTATCGCTAAAAGTAATGCATGCTTATGTGGATTCCTTTGAATTTCAAGGCTTGGAGTTTGATGAGGCCATCAGGGCCTTTCTTCAAGGCTTTAGGTTGCCTGGTGAGGCAGAGATAGATCGACAGAATAATGGAAAAGTTTGCGGAACGCTATTGCAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

576

Amino Acids

63.75

Weight (kDa)

5.2

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 166, 462, 997
AccI GTMKAC 1 cut(s) 203
AccIII TCCGGA 1 cut(s) 649
AciI CCGC 4 cut(s) 367, 515, 644, 1707
AclWI GGATC 7 cut(s) 151, 239, 252, 641, 654, 1214, 1519
AcsI RAATTY 2 cut(s) 57, 1604
AcuI CTGAAG 2 cut(s) 1353, 1382
AcyI GRCGYC 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 1088
AfaI GTAC 2 cut(s) 479, 957
AfiI CCNNNNNNNGG 8 cut(s) 166, 226, 462, 735, 997, 1268, 1313, 1436
AflIII ACRYGT 1 cut(s) 444
AjnI CCWGG 3 cut(s) 1096, 1267, 1663
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AleI CACNNNNGTG 1 cut(s) 395
Alw21I GWGCWC 1 cut(s) 601
Alw26I GTCTC 2 cut(s) 380, 395
AlwI GGATC 7 cut(s) 151, 239, 252, 641, 654, 1214, 1519
AlwNI CAGNNNCTG 1 cut(s) 166
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 5 cut(s) 496, 1066, 1347, 1629, 1638
ApeKI GCWGC 3 cut(s) 52, 76, 1493
ApoI RAATTY 2 cut(s) 57, 1604
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AseI ATTAAT 1 cut(s) 1455
AspS9I GGNCC 3 cut(s) 496, 726, 1638
AsuC2I CCSGG 1 cut(s) 1379
AsuHPI GGTGA 5 cut(s) 181, 209, 295, 1470, 1679
AvaII GGWCC 1 cut(s) 726
AxyI CCTNAGG 2 cut(s) 1125, 1482
BamHI GGATCC 2 cut(s) 244, 646
BanII GRGCYC 1 cut(s) 601
BarI GAAGNNNNNNTAC 2 cut(s) 1053, 1085
BbsI GAAGAC 3 cut(s) 626, 1268, 1566
Bbv12I GWGCWC 1 cut(s) 601
BbvI GCAGC 3 cut(s) 39, 63, 1480
BccI CCATC 6 cut(s) 235, 263, 320, 836, 1051, 1640
BceAI ACGGC 3 cut(s) 79, 156, 501
BcgI CGANNNNNNTGC 4 cut(s) 24, 34, 58, 68
BciT130I CCWGG 3 cut(s) 1098, 1269, 1665
BcnI CCSGG 1 cut(s) 1379
BcoDI GTCTC 2 cut(s) 380, 395
BfmI CTRYAG 1 cut(s) 77
BglI GCCNNNNNGGC 2 cut(s) 504, 1637
BglII AGATCT 1 cut(s) 993
BisI GCNGC 4 cut(s) 53, 77, 368, 1494
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 4 cut(s) 54, 78, 369, 1495
BmcAI AGTACT 1 cut(s) 957
Bme1390I CCNGG 4 cut(s) 1098, 1269, 1379, 1665
Bme18I GGWCC 1 cut(s) 726
BmgT120I GGNCC 3 cut(s) 496, 726, 1638
BmiI GGNNCC 4 cut(s) 210, 246, 648, 1305
BmrFI CCNGG 4 cut(s) 1098, 1269, 1379, 1665
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 4 cut(s) 153, 253, 442, 1345
BmuI ACTGGG 1 cut(s) 472
BpiI GAAGAC 3 cut(s) 626, 1268, 1566
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 1 cut(s) 1324
Bpu1102I GCTNAGC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 989
BpuMI CCSGG 1 cut(s) 1379
Bsa29I ATCGAT 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 149
BsaI GGTCTC 2 cut(s) 380, 395
BsaJI CCNNGG 2 cut(s) 1281, 1546
BsaWI WCCGGW 2 cut(s) 247, 649
Bsc4I CCNNNNNNNGG 8 cut(s) 166, 226, 462, 735, 997, 1268, 1313, 1436
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 2 cut(s) 467, 1341
Bse21I CCTNAGG 2 cut(s) 1125, 1482
Bse3DI GCAATG 1 cut(s) 362
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 3 cut(s) 1098, 1269, 1665
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 2 cut(s) 1281, 1546
BseGI GGATG 3 cut(s) 457, 1062, 1200
BseLI CCNNNNNNNGG 8 cut(s) 166, 226, 462, 735, 997, 1268, 1313, 1436
BseMI GCAATG 1 cut(s) 362
BseMII CTCAG 5 cut(s) 225, 919, 1139, 1242, 1473
BseNI ACTGG 2 cut(s) 467, 1341
BseRI GAGGAG 2 cut(s) 1316, 1499
BseXI GCAGC 3 cut(s) 39, 63, 1480
BsgI GTGCAG 2 cut(s) 311, 1065
Bsh1285I CGRYCG 1 cut(s) 1185
BshFI GGCC 5 cut(s) 498, 1068, 1349, 1631, 1640
BshVI ATCGAT 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 1185
BsiHKAI GWGCWC 1 cut(s) 601
BsiSI CCGG 4 cut(s) 248, 650, 711, 1378
BslFI GGGAC 1 cut(s) 1076
BslI CCNNNNNNNGG 8 cut(s) 166, 226, 462, 735, 997, 1268, 1313, 1436
BsmAI GTCTC 2 cut(s) 380, 395
BsmFI GGGAC 1 cut(s) 1076
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 5 cut(s) 498, 1068, 1349, 1631, 1640
Bso31I GGTCTC 2 cut(s) 380, 395
Bsp1286I GDGCHC 1 cut(s) 601
Bsp13I TCCGGA 1 cut(s) 649
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 9 cut(s) 156, 171, 244, 646, 993, 1182, 1219, 1511, 1681
Bsp1720I GCTNAGC 1 cut(s) 234
Bsp19I CCATGG 1 cut(s) 1281
BspACI CCGC 4 cut(s) 367, 515, 644, 1707
BspANI GGCC 5 cut(s) 498, 1068, 1349, 1631, 1640
BspCNI CTCAG 5 cut(s) 226, 918, 1138, 1243, 1474
BspDI ATCGAT 1 cut(s) 44
BspEI TCCGGA 1 cut(s) 649
BspLI GGNNCC 4 cut(s) 210, 246, 648, 1305
BspMAI CTGCAG 1 cut(s) 81
BspPI GGATC 7 cut(s) 151, 239, 252, 641, 654, 1214, 1519
BspTNI GGTCTC 2 cut(s) 380, 395
BsrDI GCAATG 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 710
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 2 cut(s) 467, 1341
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 2 cut(s) 1281, 1546
BssMI GATC 9 cut(s) 156, 171, 244, 646, 993, 1182, 1219, 1511, 1681
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 2 cut(s) 1281, 1546
Bst2UI CCWGG 3 cut(s) 1098, 1269, 1665
Bst4CI ACNGT 4 cut(s) 208, 397, 777, 788
Bst6I CTCTTC 1 cut(s) 568
BstACI GRCGYC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 74, 1584
BstDEI CTNAG 7 cut(s) 234, 354, 905, 1125, 1162, 1251, 1482
BstDSI CCRYGG 1 cut(s) 1281
BstENI CCTNNNNNAGG 3 cut(s) 1266, 1311, 1434
BstF5I GGATG 3 cut(s) 457, 1062, 1200
BstKTI GATC 9 cut(s) 159, 174, 247, 649, 996, 1185, 1222, 1514, 1684
BstMAI GTCTC 2 cut(s) 380, 395
BstMBI GATC 9 cut(s) 156, 171, 244, 646, 993, 1182, 1219, 1511, 1681
BstMCI CGRYCG 1 cut(s) 1185
BstMWI GCNNNNNNNGC 3 cut(s) 504, 1355, 1637
BstNI CCWGG 3 cut(s) 1098, 1269, 1665
BstNSI RCATGY 2 cut(s) 448, 1586
BstSCI CCNGG 4 cut(s) 1096, 1267, 1377, 1663
BstSFI CTRYAG 1 cut(s) 77
BstV1I GCAGC 3 cut(s) 39, 63, 1480
BstV2I GAAGAC 3 cut(s) 626, 1268, 1566
BstX2I RGATCY 5 cut(s) 244, 646, 993, 1219, 1511
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 5 cut(s) 244, 646, 993, 1219, 1511
Bsu15I ATCGAT 1 cut(s) 44
Bsu36I CCTNAGG 2 cut(s) 1125, 1482
BsuRI GGCC 5 cut(s) 498, 1068, 1349, 1631, 1640
BsuTUI ATCGAT 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 1281
BtsCI GGATG 3 cut(s) 457, 1062, 1200
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 5 cut(s) 42, 402, 773, 793, 1348
Cac8I GCNNGC 2 cut(s) 74, 1584
CaiI CAGNNNCTG 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 3 cut(s) 496, 726, 1638
ClaI ATCGAT 1 cut(s) 44
CseI GACGC 1 cut(s) 138
Csp6I GTAC 2 cut(s) 478, 956
CspCI CAANNNNNGTGG 4 cut(s) 210, 245, 1449, 1484
CviAII CATG 3 cut(s) 445, 1282, 1583
CviQI GTAC 2 cut(s) 478, 956
DdeI CTNAG 7 cut(s) 234, 354, 905, 1125, 1162, 1251, 1482
DpnI GATC 9 cut(s) 158, 173, 246, 648, 995, 1184, 1221, 1513, 1683
DpnII GATC 9 cut(s) 156, 171, 244, 646, 993, 1182, 1219, 1511, 1681
DraIII CACNNNGTG 1 cut(s) 1088
Eam1104I CTCTTC 1 cut(s) 568
EarI CTCTTC 1 cut(s) 568
EciI GGCGGA 2 cut(s) 530, 659
Ecl136II GAGCTC 1 cut(s) 599
Eco130I CCWWGG 2 cut(s) 1281, 1546
Eco147I AGGCCT 1 cut(s) 1349
Eco24I GRGCYC 1 cut(s) 601
Eco31I GGTCTC 2 cut(s) 380, 395
Eco32I GATATC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 726
Eco53kI GAGCTC 1 cut(s) 599
Eco57I CTGAAG 2 cut(s) 1353, 1382
Eco81I CCTNAGG 2 cut(s) 1125, 1482
EcoICRI GAGCTC 1 cut(s) 599
EcoNI CCTNNNNNAGG 3 cut(s) 1266, 1311, 1434
EcoO109I RGGNCCY 1 cut(s) 1638
EcoRI GAATTC 1 cut(s) 57
EcoRII CCWGG 3 cut(s) 1096, 1267, 1663
EcoRV GATATC 1 cut(s) 48
EcoT14I CCWWGG 2 cut(s) 1281, 1546
EcoT22I ATGCAT 1 cut(s) 1584
EcoT38I GRGCYC 1 cut(s) 601
ErhI CCWWGG 2 cut(s) 1281, 1546
FaeI CATG 3 cut(s) 448, 1285, 1586
FaqI GGGAC 1 cut(s) 1076
FatI CATG 3 cut(s) 444, 1281, 1582
FblI GTMKAC 1 cut(s) 203
Fnu4HI GCNGC 4 cut(s) 53, 77, 368, 1494
FokI GGATG 3 cut(s) 464, 1069, 1207
FriOI GRGCYC 1 cut(s) 601
Fsp4HI GCNGC 4 cut(s) 53, 77, 368, 1494
GluI GCNGC 4 cut(s) 53, 77, 368, 1494
GsuI CTGGAG 1 cut(s) 1324
HaeIII GGCC 5 cut(s) 498, 1068, 1349, 1631, 1640
HapII CCGG 4 cut(s) 248, 650, 711, 1378
HgaI GACGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 3 cut(s) 448, 1285, 1586
HincII GTYRAC 2 cut(s) 204, 1003
HindII GTYRAC 2 cut(s) 204, 1003
HinfI GANTC 5 cut(s) 197, 428, 622, 986, 1595
HpaII CCGG 4 cut(s) 248, 650, 711, 1378
HphI GGTGA 5 cut(s) 181, 209, 295, 1470, 1679
Hpy166II GTNNAC 3 cut(s) 204, 1003, 1478
Hpy188III TCNNGA 8 cut(s) 160, 650, 849, 968, 1127, 1217, 1262, 1400
Hpy8I GTNNAC 3 cut(s) 204, 1003, 1478
Hpy99I CGWCG 4 cut(s) 154, 205, 208, 377
HpyAV CCTTC 6 cut(s) 139, 351, 655, 1079, 1307, 1397
HpyCH4III ACNGT 4 cut(s) 208, 397, 777, 788
HpyCH4V TGCA 6 cut(s) 79, 292, 1082, 1460, 1582, 1720
HpyF10VI GCNNNNNNNGC 3 cut(s) 504, 1355, 1637
HpyF3I CTNAG 7 cut(s) 234, 354, 905, 1125, 1162, 1251, 1482
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 3 cut(s) 448, 1285, 1586
Kpn2I TCCGGA 1 cut(s) 649
Kzo9I GATC 9 cut(s) 156, 171, 244, 646, 993, 1182, 1219, 1511, 1681
LmnI GCTCC 4 cut(s) 4, 682, 830, 1303
Lsp1109I GCAGC 3 cut(s) 39, 63, 1480
LweI GCATC 4 cut(s) 153, 253, 442, 1345
MaeIII GTNAC 2 cut(s) 37, 771
MalI GATC 9 cut(s) 158, 173, 246, 648, 995, 1184, 1221, 1513, 1683
MboI GATC 9 cut(s) 156, 171, 244, 646, 993, 1182, 1219, 1511, 1681
MboII GAAGA 9 cut(s) 195, 585, 631, 703, 823, 958, 1268, 1571, 1637
MfeI CAATTG 2 cut(s) 1205, 1365
MflI RGATCY 5 cut(s) 244, 646, 993, 1219, 1511
MhlI GDGCHC 1 cut(s) 601
MlyI GAGTC 2 cut(s) 191, 616
MmeI TCCRAC 3 cut(s) 342, 429, 681
Mph1103I ATGCAT 1 cut(s) 1584
MroI TCCGGA 1 cut(s) 649
MseI TTAA 5 cut(s) 129, 753, 779, 1074, 1455
MslI CAYNNNNRTG 2 cut(s) 395, 1587
MspI CCGG 4 cut(s) 248, 650, 711, 1378
MspR9I CCNGG 4 cut(s) 1098, 1269, 1379, 1665
MunI CAATTG 2 cut(s) 1205, 1365
Mva1269I GAATGC 1 cut(s) 712
MvaI CCWGG 3 cut(s) 1098, 1269, 1665
MwoI GCNNNNNNNGC 3 cut(s) 504, 1355, 1637
NciI CCSGG 1 cut(s) 1379
NcoI CCATGG 1 cut(s) 1281
NdeII GATC 9 cut(s) 156, 171, 244, 646, 993, 1182, 1219, 1511, 1681
NlaIII CATG 3 cut(s) 448, 1285, 1586
NlaIV GGNNCC 4 cut(s) 210, 246, 648, 1305
NmuCI GTSAC 2 cut(s) 37, 771
NsiI ATGCAT 1 cut(s) 1584
NspI RCATGY 2 cut(s) 448, 1586
OliI CACNNNNGTG 1 cut(s) 395
PaeI GCATGC 1 cut(s) 1586
PceI AGGCCT 1 cut(s) 1349
PciI ACATGT 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PctI GAATGC 1 cut(s) 712
PfeI GAWTC 3 cut(s) 428, 986, 1595
PflMI CCANNNNNTGG 3 cut(s) 166, 462, 997
PkrI GCNGC 4 cut(s) 54, 78, 369, 1495
Ple19I CGATCG 1 cut(s) 1185
PleI GAGTC 2 cut(s) 191, 616
PpsI GAGTC 2 cut(s) 191, 616
PscI ACATGT 1 cut(s) 444
PshBI ATTAAT 1 cut(s) 1455
Psp124BI GAGCTC 1 cut(s) 601
Psp6I CCWGG 3 cut(s) 1096, 1267, 1663
PspGI CCWGG 3 cut(s) 1096, 1267, 1663
PspN4I GGNNCC 4 cut(s) 210, 246, 648, 1305
PspPI GGNCC 3 cut(s) 496, 726, 1638
PstI CTGCAG 1 cut(s) 81
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 5 cut(s) 244, 646, 993, 1219, 1511
PvuI CGATCG 1 cut(s) 1185
RsaI GTAC 2 cut(s) 479, 957
RsaNI GTAC 2 cut(s) 478, 956
RseI CAYNNNNRTG 2 cut(s) 395, 1587
SacI GAGCTC 1 cut(s) 601
SalI GTCGAC 1 cut(s) 202
SaqAI TTAA 5 cut(s) 129, 753, 779, 1074, 1455
SatI GCNGC 4 cut(s) 53, 77, 368, 1494
Sau3AI GATC 9 cut(s) 156, 171, 244, 646, 993, 1182, 1219, 1511, 1681
Sau96I GGNCC 3 cut(s) 496, 726, 1638
ScaI AGTACT 1 cut(s) 957
SchI GAGTC 2 cut(s) 191, 616
ScrFI CCNGG 4 cut(s) 1098, 1269, 1379, 1665
SduI GDGCHC 1 cut(s) 601
SfaNI GCATC 4 cut(s) 153, 253, 442, 1345
SfcI CTRYAG 1 cut(s) 77
SfiI GGCCNNNNNGGCC 1 cut(s) 1637
SgrDI CGTCGACG 1 cut(s) 202
SinI GGWCC 1 cut(s) 726
SmiMI CAYNNNNRTG 2 cut(s) 395, 1587
SmlI CTYRAG 1 cut(s) 968
SmoI CTYRAG 1 cut(s) 968
SphI GCATGC 1 cut(s) 1586
SseBI AGGCCT 1 cut(s) 1349
SsiI CCGC 4 cut(s) 367, 515, 644, 1707
SspI AATATT 1 cut(s) 761
SstI GAGCTC 1 cut(s) 601
StuI AGGCCT 1 cut(s) 1349
StyD4I CCNGG 4 cut(s) 1096, 1267, 1377, 1663
StyI CCWWGG 2 cut(s) 1281, 1546
TaaI ACNGT 4 cut(s) 208, 397, 777, 788
TaqI TCGA 8 cut(s) 44, 203, 275, 344, 431, 1040, 1181, 1684
TatI WGTACW 2 cut(s) 477, 955
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 3 cut(s) 428, 986, 1595
Tru1I TTAA 5 cut(s) 129, 753, 779, 1074, 1455
Tru9I TTAA 5 cut(s) 129, 753, 779, 1074, 1455
TscAI CASTG 5 cut(s) 42, 402, 780, 793, 1348
TseFI GTSAC 2 cut(s) 37, 771
TseI GCWGC 3 cut(s) 52, 76, 1493
Tsp45I GTSAC 2 cut(s) 37, 771
TspDTI ATGAA 7 cut(s) 632, 674, 823, 1026, 1154, 1169, 1211
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 5 cut(s) 42, 402, 780, 793, 1348
Van91I CCANNNNNTGG 3 cut(s) 166, 462, 997
VpaK11BI GGWCC 1 cut(s) 726
VspI ATTAAT 1 cut(s) 1455
XagI CCTNNNNNAGG 3 cut(s) 1266, 1311, 1434
XapI RAATTY 2 cut(s) 57, 1604
XceI RCATGY 2 cut(s) 448, 1586
XmiI GTMKAC 1 cut(s) 203
ZrmI AGTACT 1 cut(s) 957
Zsp2I ATGCAT 1 cut(s) 1584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.