Rh1CG212700

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0007735
Physical Location & Seq
Forward (+)
45891107 .. 45900682
9576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG212700.1

Sequence Viewer

Length: 4080 bp
ATGATAGTTTTTCAGCTATCTTGTTCCATCTTCATTAGTCTGGTGTCAAGATTTAGAGCTGGATTGAAAGCAGAGATTGGAGTATTCTTTCCTATGATTGTTCTCAGAGTTTTAGAGAATGTTGCACAACCTAATTTTCAGCAGAAGATGATAGTGCTTCGGTTTCTTGAGAAGCTCTGTGTTGATTCCCAGATCTTGGTCGACATATTTATCAATTATGATTGCGATGTCAATTCATCAAACATATTTGAGAGGATGGTAAATGGCCTTCTTAAAACTGCCCAAGGTGTCCCTCCTGGTGTTGCCACCACGTTATTACCTCCTCAAGAGGCAAATATGAAACTTGAAGCCATGAGGTGTTTAGTTGCTATTCTGAGGTCAATCGGGGACTGGATGAACAAACAATTGCGCATTCCAGATCCTCATTCTACCAAGAAATCTGAAGTAAATGAGAATAGTTCTGAACCAGGAGCTCTTCCCATGGCAAATGGGAATGGTGAGGAGTCTGTTGAAGGATCAGATTCTCATTCTGAAGCCTCCAGTGAAGCCTCTGATGCTTTGACAATCGAGCAACGCCGAGCTTACAAGCTGGAACTTCAGGAAGGTATATCTCTTTTCAATCGGAAACCTAAGAAAGGAATTGAGTTTCTTATCAATGCAAATAAGGTGGGCAATTCACCCGAGGAGATAGCTGCTTTTCTAAAAAATGCATCTGGTTTGAACAAGACTTTGATTGGAGACTACCTTGGAGAAAGAGAAGACTTATCGCTAAAAGTAATGCATGCTTATGTGGATTCCTTTGATTTTCAAGACTTGGAGTTTGATGAGGCAATCAGGTCCTTTCTTCAAGGTTTTAGGTTGCCTGGTGAGGCACAGAAGATTGACAGAATAATGGAAAAGTTTGCTGAGCGCTTTTGCAAGTGCAATCCAAAGGCTTTTACTAGTGCTGATACAGCCTATGTCCTTGCTTACTCCGTGATACTGCTCAATACTGATGCTCATAACCCGATGGTGAAGAGCAAGATGTCAGCTGATGATTTTATAAGAAACAATCGTGGCATAGATGATGGAAAAGATCTACCTGAGCAGTACTTGAGGTCATTGTATGAACGAATATCAAGAAAGGAAATAAAAATGAAAGAATATGATTTGGCTCCTCAGCAGATACAGTCGGTGAACCCTAACCGTCTTTTGGGCTTGGATAGTATCTTGAATATTGTGATCCGTAAACGGGGGGATAGTCAATTGGAAACCAGTGATGATCTTATCAAGCATATGCAAGAGCAATTCCAAGAAAAAGCTCGGAAATCTGAGTCAGTTTACTATGCTGCAACAGATGTGGTAATTCTTAGATTCATGGTTGAAGTCTGCTGGGCTCCTATGCTGGCTGCCTTCAGTGTTCCACTTGACCAAACTGATGATGAAGTAGTTATTTCTCTGTGTCTTGAAGGGATTCGTTATGCTATCCATGTAACTGCCGCAATGTCCATGAAGACCCATAGAGATGCTTTTGTGACTTCACTGGCGAAGTTTACTTCCCTCCACTCTCCTGCTGATATCAAGCAGAAAAACATAGAAGCAATCAAGGCAATAGTGACAATTGCAGATGAAGATGGGAACTACCTGCAAGAAGCTTGGGAGCATATTTTGACATGTGTTTCCCGCTTTGAACATCTTCATCTCCTGGGAGAGGGTGCTCCTCCAGATGCTACTTTCTTTGCTTTTCCTCAGAATGAATCAGAAAAATCAAAGCAAGCCAAGTCAACTGTCCTTCCTGTTTTAAAAAAGAAGGGCCAAGGGAAGATGCAGTATGCTGCTGCTGCCGTGTTGAGAGGTTCATATGATAGTGCTGGTATTGGTGGGAATTCTTCTGGGATGGTCACATCAGAACAGATGAACAATTTGGTTTCTAACTTAAATATGTTAGAACAAGTTGGAGACATGAGCCGCATATTCACACGGAGTCAAAAGCTGAACAGTGAGGCAATTATAGATTTTGTTAAGGCTCTTTGCAAGGTCTCTATGGAGGAATTGCGATCTGCATCTGATCCTCGGGTTTTCAGCCTTACGAAGATGGTTGAGATTGCGCATTATAACATGAACCGCATCAGGCTTGTGTGGTCTAGCATCTGGCATGTACTCTCTAATTTCTTCGTGACAATTGGCTGTTCTGAAAACCTGTCTATTGCAATTTTTGCTATGGATTCTCTACGCCAATTGTCAATGAAATTCTTGGAGCGAGAAGAGCTGGCTAATTATAATTTTCAGAATGAGTTTATGAAGCCTTTTGTCATTGTTATGCGTAAAAGTAGTGCAGTTGAGATACGAGAATTAATCATCAGATGTGTCTCCCAAATGGTTTTATCTCGTGTGAATAGAGTCAAATCTGGATGGAAGAGCATGTTCATGGTCTTCACTACGGCAGCTTATGATGACCACAAAAATATTGTACTCTTGGCCTTTGAAATTATTGAGAAGATTATTCGCGACTACTTTCCGTACATCACCGAGACTGAAACCACTACTTTTACTGATTGCGTAAATTGTCTAATTGCATTCACCAATAGTAGATTTAACAAAGACATTAGCCTCAATGCTATTGCTTTTCTTCGGTTCTGTGCAACAAAACTGGCCGAGGGAGGTCTTAGTTCCTCATCAAGAAATAAGGACAAGGATGCATCTGGAAAAATATCTCCAACGTCACCTCAAGGATGGAAAGAAGGAAGACAAGATAATGGGGGAATGCCAGACAAGGATGACCATCTTTATTTCTGGTTCCCTTTATTGGCTGGTTTGTCCGAACTTAGCTTTGATCCTAGGCCTGAAATCAGGAAGAGTGCCTTACAAGTGCTGTTTGAAACTCTACGTAACCATGGTCACCTTTTCTCTCTACCTTTGTGGGAAAAGGTGTTTGAATCAGTGCTATTTCCAATATTTGACTATGTGCGCCATGCTATTGATCCTTCTGGGGAAAGTTCACCAGAGCAAGGGATTGACAGTGAAACAGGCGAGCTTGATCAAGATGCCTGGATGTACGAGACATGCACATTGGCACTCCAATTAGTTGTTGATCTTTTTGTCAAGTTTTATGACACTGTCAATCCTCTTTTGAAGAAAGTTTTGGATCTTCTAGTCAGTTTTATTGGACGACCTCACCAAAGCCTAGCTGGCATAGGTATTGCTGCATTTGTGCGATTGATGAGCAATGCAGGGGATCTCTTTTCTGATGAGAAGTGGCTTGAAGTGGTTTCATCATTAAAAGAAGCTGCCAATTCAACTCTTCCTGATTTTTCTTTCATTCTTAACGGAGATAGCATCATGGGAATCCTAGACTCTTCTTCTAGTAGAGAAGATCATGGAGGGTCTGGGAGGCCTGATGATGATTCAGAGAGACTAAGGACAAACCATCTTTATGCTGGCTTGGCTGACGTTAAGTGCCGAGCTGCTGTTCAGCTATTATTGATTCAGGCAGTGATGGAGATTTACACCATGTATCGTTCTCACCTTTCATCGAATAACACATTAATCCTCTTCAATGCTCTGCATGATTTGGCATCCCATGCTCACAAAGTTAACACTGATACCACTTTACGGGCAAGGCTACAAGAGTTTGGCTCCATGACGCAAATGCAAGACCCTCCACTGTTACGTATTGAGAACGAATCTTACCAAATCTGCCTTACATTCTTACAGAATCTTGTAGAGGACAGGCCTCCAAGTTATGATGAGGTGGAGGTAGAGTCCCACATTGTGGACCTTTGCAAGGAGGTTTTACAGTTCTATATTGAAGCCGCTAGCTCTGGGAAGATATCTGAATCATCTAATGGGCAGCAGCACCATTGGCTGATCCCTCTAGGTTCTGGGCGACGGAGAGAATTGGCTGCACGCGCACCGCTTATTGTGGCAACTCTCCAGGCCATCTGTTGCTTGGGAGACACTTCATTTGAAAATAACTTGTCTCATTTCTTCCCGCTGCTTGCAACCTTGATAACTTGTGAACATGGGTCCGATGAGGTCCAGGCTGCCCTTAGTGACATGCTCAGCTCATCTGTGGGTCCTGTTTTGCTTCGATCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1359

Amino Acids

152.47

Weight (kDa)

5.45

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 3 - 82 6.6e-23 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 196 - 377 1.8e-77 Sec7 domain
Sec7-like_HDS PF09324 743 - 825 1.2e-29 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 915 - 1003 1.8e-12 C-terminal region of Mon2 protein
BIG2_C PF20252 1149 - 1348 3.4e-43 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1043, 2094, 2259
Acc16I TGCGCA 2 cut(s) 410, 2088
Acc36I ACCTGC 1 cut(s) 1632
AccB7I CCANNNNNTGG 2 cut(s) 196, 3751
AccI GTMKAC 1 cut(s) 201
AccII CGCG 2 cut(s) 2487, 3888
AciI CCGC 7 cut(s) 1479, 1663, 1948, 2104, 3792, 3893, 3971
AclWI GGATC 9 cut(s) 413, 523, 1216, 2042, 2807, 2954, 3132, 3222, 3841
AcoI YGGCCR 1 cut(s) 2631
AcsI RAATTY 2 cut(s) 1864, 2228
AcuI CTGAAG 4 cut(s) 462, 552, 581, 1378
AdeI CACNNNGTG 1 cut(s) 3751
AfaI GTAC 5 cut(s) 1091, 2139, 2451, 2501, 3035
AfeI AGCGCT 1 cut(s) 911
AflIII ACRYGT 1 cut(s) 1652
AhlI ACTAGT 1 cut(s) 941
AjnI CCWGG 7 cut(s) 295, 466, 862, 1683, 3026, 3912, 4017
AjuI GAANNNNNNNTTGG 2 cut(s) 3104, 3136
Alw21I GWGCWC 2 cut(s) 475, 1699
Alw26I GTCTC 9 cut(s) 732, 1932, 2023, 2353, 2504, 3032, 3385, 3927, 3963
AlwI GGATC 9 cut(s) 413, 523, 1216, 2042, 2807, 2954, 3132, 3222, 3841
Ama87I CYCGRG 2 cut(s) 680, 2052
Aor51HI AGCGCT 1 cut(s) 911
AoxI GGCC 8 cut(s) 265, 1792, 2457, 2631, 2819, 3371, 3710, 3915
ApoI RAATTY 2 cut(s) 1864, 2228
ArsI GACNNNNNNTTYG 4 cut(s) 803, 835, 3926, 3958
AseI ATTAAT 2 cut(s) 2333, 3524
Asp700I GAANNNNTTC 2 cut(s) 1452, 1674
AspA2I CCTAGG 1 cut(s) 2816
AspLEI GCGC 5 cut(s) 411, 912, 2089, 2949, 3890
AspS9I GGNCC 6 cut(s) 837, 1792, 3754, 4005, 4015, 4055
AsuNHI GCTAGC 1 cut(s) 3794
AvaI CYCGRG 2 cut(s) 680, 2052
AvaII GGWCC 5 cut(s) 837, 3754, 4005, 4015, 4055
AvrII CCTAGG 1 cut(s) 2816
BanII GRGCYC 2 cut(s) 475, 1378
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BauI CACGAG 1 cut(s) 2367
BbsI GAAGAC 4 cut(s) 765, 1499, 2404, 2731
Bbv12I GWGCWC 2 cut(s) 475, 1699
BbvCI CCTCAGC 1 cut(s) 1158
BceAI ACGGC 2 cut(s) 1808, 2436
BciT130I CCWGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
BclI TGATCA 1 cut(s) 3016
BcoDI GTCTC 9 cut(s) 732, 1932, 2023, 2353, 2504, 3032, 3385, 3927, 3963
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 9 cut(s) 942, 2124, 2817, 3131, 3164, 3329, 3342, 3795, 3854
BfoI RGCGCY 1 cut(s) 913
BfuAI ACCTGC 1 cut(s) 1632
BglI GCCNNNNNGGC 1 cut(s) 3168
BglII AGATCT 2 cut(s) 192, 1075
BlnI CCTAGG 1 cut(s) 2816
BlpI GCTNAGC 2 cut(s) 906, 4040
BmcAI AGTACT 1 cut(s) 1091
Bme1390I CCNGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
Bme18I GGWCC 5 cut(s) 837, 3754, 4005, 4015, 4055
BmeT110I CYCGRG 2 cut(s) 680, 2052
BmgT120I GGNCC 6 cut(s) 837, 1792, 3754, 4005, 4015, 4055
BmiI GGNNCC 6 cut(s) 1155, 1377, 2777, 3616, 4006, 4056
BmrFI CCNGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
BmtI GCTAGC 1 cut(s) 3798
BpiI GAAGAC 4 cut(s) 765, 1499, 2404, 2731
BplI GAGNNNNNCTC 2 cut(s) 3599, 3631
BpmI CTGGAG 3 cut(s) 523, 1686, 3896
Bpu10I CCTNAGC 2 cut(s) 1083, 1158
Bpu1102I GCTNAGC 2 cut(s) 906, 4040
BpuEI CTTGAG 4 cut(s) 188, 309, 1114, 2691
BsaAI YACGTR 2 cut(s) 2867, 3650
BsaBI GATNNNNATC 2 cut(s) 2041, 2760
BsaI GGTCTC 1 cut(s) 2023
BsaXI ACNNNNNCTCC 2 cut(s) 3725, 3755
Bse1I ACTGG 5 cut(s) 395, 540, 1254, 1527, 2634
Bse3DI GCAATG 2 cut(s) 1488, 3211
Bse8I GATNNNNATC 2 cut(s) 2041, 2760
BseBI CCWGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
BseGI GGATG 9 cut(s) 261, 399, 1881, 2396, 2680, 2717, 2761, 3036, 3554
BseJI GATNNNNATC 2 cut(s) 2041, 2760
BseMI GCAATG 2 cut(s) 1488, 3211
BseMII CTCAG 8 cut(s) 118, 365, 897, 1074, 1172, 1302, 1742, 4054
BseNI ACTGG 5 cut(s) 395, 540, 1254, 1527, 2634
BseRI GAGGAG 5 cut(s) 312, 515, 698, 1146, 1689
BseYI CCCAGC 1 cut(s) 1371
BsgI GTGCAG 2 cut(s) 2334, 3867
Bsh1236I CGCG 2 cut(s) 2487, 3888
BshFI GGCC 8 cut(s) 267, 1794, 2459, 2633, 2821, 3373, 3712, 3917
BsiHKAI GWGCWC 2 cut(s) 475, 1699
BsiHKCI CYCGRG 2 cut(s) 680, 2052
BslFI GGGAC 3 cut(s) 275, 401, 3727
BsmAI GTCTC 9 cut(s) 732, 1932, 2023, 2353, 2504, 3032, 3385, 3927, 3963
BsmFI GGGAC 3 cut(s) 275, 401, 3727
BsmI GAATGC 3 cut(s) 411, 2555, 2748
BsnI GGCC 8 cut(s) 267, 1794, 2459, 2633, 2821, 3373, 3712, 3917
Bso31I GGTCTC 1 cut(s) 2023
BsoBI CYCGRG 2 cut(s) 680, 2052
Bsp1286I GDGCHC 3 cut(s) 475, 1378, 1699
Bsp1720I GCTNAGC 2 cut(s) 906, 4040
Bsp19I CCATGG 2 cut(s) 480, 2872
Bsp68I TCGCGA 1 cut(s) 2487
BspACI CCGC 7 cut(s) 1479, 1663, 1948, 2104, 3792, 3893, 3971
BspANI GGCC 8 cut(s) 267, 1794, 2459, 2633, 2821, 3373, 3712, 3917
BspCNI CTCAG 8 cut(s) 117, 366, 898, 1075, 1171, 1303, 1741, 4053
BspFNI CGCG 2 cut(s) 2487, 3888
BspLI GGNNCC 6 cut(s) 1155, 1377, 2777, 3616, 4006, 4056
BspMI ACCTGC 1 cut(s) 1632
BspOI GCTAGC 1 cut(s) 3798
BspPI GGATC 9 cut(s) 413, 523, 1216, 2042, 2807, 2954, 3132, 3222, 3841
BspQI GCTCTTC 4 cut(s) 480, 1010, 2238, 2390
BspTNI GGTCTC 1 cut(s) 2023
BsrDI GCAATG 2 cut(s) 1488, 3211
BsrI ACTGG 5 cut(s) 395, 540, 1254, 1527, 2634
BssSI CACGAG 1 cut(s) 2367
BssT1I CCWWGG 6 cut(s) 283, 480, 745, 1795, 2816, 2872
Bst2BI CACGAG 1 cut(s) 2367
Bst2UI CCWGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
Bst4CI ACNGT 8 cut(s) 1170, 1187, 1768, 1979, 2999, 3097, 3645, 3777
Bst6I CTCTTC 8 cut(s) 480, 1010, 2238, 2390, 2828, 3285, 3340, 3536
BstAPI GCANNNNNTGC 2 cut(s) 2195, 3560
BstBAI YACGTR 2 cut(s) 2867, 3650
BstDSI CCRYGG 2 cut(s) 480, 2872
BstEII GGTNACC 1 cut(s) 2876
BstENI CCTNNNNNAGG 3 cut(s) 633, 1688, 3761
BstF5I GGATG 9 cut(s) 261, 399, 1881, 2396, 2680, 2717, 2761, 3036, 3554
BstFNI CGCG 2 cut(s) 2487, 3888
BstH2I RGCGCY 1 cut(s) 913
BstHHI GCGC 5 cut(s) 411, 912, 2089, 2949, 3890
BstMAI GTCTC 9 cut(s) 732, 1932, 2023, 2353, 2504, 3032, 3385, 3927, 3963
BstNI CCWGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
BstNSI RCATGY 6 cut(s) 785, 1656, 2138, 2404, 3045, 4039
BstPI GGTNACC 1 cut(s) 2876
BstSCI CCNGG 7 cut(s) 295, 466, 862, 1683, 3026, 3912, 4017
BstSNI TACGTA 2 cut(s) 2867, 3650
BstUI CGCG 2 cut(s) 2487, 3888
BstV2I GAAGAC 4 cut(s) 765, 1499, 2404, 2731
BstX2I RGATCY 5 cut(s) 192, 418, 1075, 3124, 3214
BstYI RGATCY 5 cut(s) 192, 418, 1075, 3124, 3214
BsuRI GGCC 8 cut(s) 267, 1794, 2459, 2633, 2821, 3373, 3712, 3917
BtgI CCRYGG 2 cut(s) 480, 2872
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 9 cut(s) 261, 399, 1881, 2396, 2680, 2717, 2761, 3036, 3554
BtsI GCAGTG 1 cut(s) 3477
BtuMI TCGCGA 1 cut(s) 2487
BveI ACCTGC 1 cut(s) 1632
CfoI GCGC 5 cut(s) 411, 912, 2089, 2949, 3890
Cfr13I GGNCC 6 cut(s) 837, 1792, 3754, 4005, 4015, 4055
CseI GACGC 1 cut(s) 3631
Csp6I GTAC 5 cut(s) 1090, 2138, 2450, 2500, 3034
CspCI CAANNNNNGTGG 4 cut(s) 648, 683, 1320, 1355
CviQI GTAC 5 cut(s) 1090, 2138, 2450, 2500, 3034
DraI TTTAAA 1 cut(s) 1782
DraIII CACNNNGTG 1 cut(s) 3751
EaeI YGGCCR 1 cut(s) 2631
Eam1104I CTCTTC 8 cut(s) 480, 1010, 2238, 2390, 2828, 3285, 3340, 3536
EarI CTCTTC 8 cut(s) 480, 1010, 2238, 2390, 2828, 3285, 3340, 3536
Ecl136II GAGCTC 1 cut(s) 473
Eco105I TACGTA 2 cut(s) 2867, 3650
Eco130I CCWWGG 6 cut(s) 283, 480, 745, 1795, 2816, 2872
Eco147I AGGCCT 3 cut(s) 2821, 3373, 3712
Eco24I GRGCYC 2 cut(s) 475, 1378
Eco31I GGTCTC 1 cut(s) 2023
Eco32I GATATC 2 cut(s) 1558, 3810
Eco47I GGWCC 5 cut(s) 837, 3754, 4005, 4015, 4055
Eco47III AGCGCT 1 cut(s) 911
Eco53kI GAGCTC 1 cut(s) 473
Eco57I CTGAAG 4 cut(s) 462, 552, 581, 1378
Eco88I CYCGRG 2 cut(s) 680, 2052
Eco91I GGTNACC 1 cut(s) 2876
EcoICRI GAGCTC 1 cut(s) 473
EcoNI CCTNNNNNAGG 3 cut(s) 633, 1688, 3761
EcoO109I RGGNCCY 2 cut(s) 837, 4055
EcoO65I GGTNACC 1 cut(s) 2876
EcoRI GAATTC 1 cut(s) 1864
EcoRII CCWGG 7 cut(s) 295, 466, 862, 1683, 3026, 3912, 4017
EcoRV GATATC 2 cut(s) 1558, 3810
EcoT14I CCWWGG 6 cut(s) 283, 480, 745, 1795, 2816, 2872
EcoT22I ATGCAT 3 cut(s) 712, 783, 2680
EcoT38I GRGCYC 2 cut(s) 475, 1378
ErhI CCWWGG 6 cut(s) 283, 480, 745, 1795, 2816, 2872
FalI AAGNNNNNCTT 2 cut(s) 2825, 2857
FaqI GGGAC 3 cut(s) 275, 401, 3727
FauI CCCGC 2 cut(s) 1670, 3978
FauNDI CATATG 2 cut(s) 1275, 1840
FbaI TGATCA 1 cut(s) 3016
FblI GTMKAC 1 cut(s) 201
FokI GGATG 9 cut(s) 268, 406, 1888, 2403, 2687, 2724, 2768, 3043, 3541
FriOI GRGCYC 2 cut(s) 475, 1378
FspBI CTAG 9 cut(s) 942, 2124, 2817, 3131, 3164, 3329, 3342, 3795, 3854
FspI TGCGCA 2 cut(s) 410, 2088
GlaI GCGC 5 cut(s) 410, 911, 2088, 2948, 3889
GsaI CCCAGC 1 cut(s) 1375
GsuI CTGGAG 3 cut(s) 523, 1686, 3896
HaeII RGCGCY 1 cut(s) 913
HaeIII GGCC 8 cut(s) 267, 1794, 2459, 2633, 2821, 3373, 3712, 3917
HgaI GACGC 1 cut(s) 3631
HhaI GCGC 5 cut(s) 411, 912, 2089, 2949, 3890
Hin6I GCGC 5 cut(s) 409, 910, 2087, 2947, 3888
HinP1I GCGC 5 cut(s) 409, 910, 2087, 2947, 3888
HincII GTYRAC 3 cut(s) 202, 1764, 3574
HindII GTYRAC 3 cut(s) 202, 1764, 3574
HindIII AAGCTT 1 cut(s) 1632
HpaI GTTAAC 1 cut(s) 3574
Hpy99I CGWCG 1 cut(s) 3870
HpyAV CCTTC 9 cut(s) 278, 506, 596, 1402, 1442, 1781, 1783, 2714, 2973
HpyCH4III ACNGT 8 cut(s) 1170, 1187, 1768, 1979, 2999, 3097, 3645, 3777
HpyCH4IV ACGT 5 cut(s) 311, 2699, 2866, 3429, 3649
HpySE526I ACGT 5 cut(s) 311, 2699, 2866, 3429, 3649
HspAI GCGC 5 cut(s) 409, 910, 2087, 2947, 3888
Ksp22I TGATCA 1 cut(s) 3016
KspAI GTTAAC 1 cut(s) 3574
LguI GCTCTTC 4 cut(s) 480, 1010, 2238, 2390
LmnI GCTCC 7 cut(s) 470, 1159, 1381, 1639, 1702, 2236, 3620
MaeI CTAG 9 cut(s) 942, 2124, 2817, 3131, 3164, 3329, 3342, 3795, 3854
MaeII ACGT 5 cut(s) 311, 2699, 2866, 3429, 3649
MfeI CAATTG 5 cut(s) 404, 1244, 1599, 2160, 2216
MflI RGATCY 5 cut(s) 192, 418, 1075, 3124, 3214
MhlI GDGCHC 3 cut(s) 475, 1378, 1699
MlyI GAGTC 6 cut(s) 512, 1322, 1972, 2388, 3326, 3749
MmeI TCCRAC 2 cut(s) 1915, 2720
Mph1103I ATGCAT 3 cut(s) 712, 783, 2680
MroXI GAANNNNTTC 2 cut(s) 1452, 1674
MslI CAYNNNNRTG 5 cut(s) 786, 1503, 2297, 2405, 3411
MspA1I CMGCKG 2 cut(s) 1031, 3973
MspR9I CCNGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
MunI CAATTG 5 cut(s) 404, 1244, 1599, 2160, 2216
Mva1269I GAATGC 3 cut(s) 411, 2555, 2748
MvaI CCWGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
MvnI CGCG 2 cut(s) 2487, 3888
NcoI CCATGG 2 cut(s) 480, 2872
NdeI CATATG 2 cut(s) 1275, 1840
NheI GCTAGC 1 cut(s) 3794
NlaIV GGNNCC 6 cut(s) 1155, 1377, 2777, 3616, 4006, 4056
NmeAIII GCCGAG 3 cut(s) 602, 2659, 3464
NmuCI GTSAC 7 cut(s) 1513, 1594, 1879, 2155, 2700, 2876, 4031
NruI TCGCGA 1 cut(s) 2487
NsbI TGCGCA 2 cut(s) 410, 2088
NsiI ATGCAT 3 cut(s) 712, 783, 2680
NspI RCATGY 6 cut(s) 785, 1656, 2138, 2404, 3045, 4039
PaeI GCATGC 1 cut(s) 785
PceI AGGCCT 3 cut(s) 2821, 3373, 3712
PciI ACATGT 1 cut(s) 1652
PciSI GCTCTTC 4 cut(s) 480, 1010, 2238, 2390
PctI GAATGC 3 cut(s) 411, 2555, 2748
PdmI GAANNNNTTC 2 cut(s) 1452, 1674
PflFI GACNNNGTC 1 cut(s) 3095
PflMI CCANNNNNTGG 2 cut(s) 196, 3751
PleI GAGTC 6 cut(s) 511, 1321, 1971, 2387, 3326, 3748
PpsI GAGTC 6 cut(s) 511, 1321, 1971, 2387, 3326, 3748
Ppu21I YACGTR 2 cut(s) 2867, 3650
PpuMI RGGWCCY 2 cut(s) 837, 4055
PscI ACATGT 1 cut(s) 1652
PshBI ATTAAT 2 cut(s) 2333, 3524
PsiI TTATAA 3 cut(s) 1043, 2094, 2259
Psp124BI GAGCTC 1 cut(s) 475
Psp5II RGGWCCY 2 cut(s) 837, 4055
Psp6I CCWGG 7 cut(s) 295, 466, 862, 1683, 3026, 3912, 4017
PspEI GGTNACC 1 cut(s) 2876
PspFI CCCAGC 1 cut(s) 1371
PspGI CCWGG 7 cut(s) 295, 466, 862, 1683, 3026, 3912, 4017
PspN4I GGNNCC 6 cut(s) 1155, 1377, 2777, 3616, 4006, 4056
PspPI GGNCC 6 cut(s) 837, 1792, 3754, 4005, 4015, 4055
PspPPI RGGWCCY 2 cut(s) 837, 4055
PsrI GAACNNNNNNTAC 2 cut(s) 3650, 3682
PsuI RGATCY 5 cut(s) 192, 418, 1075, 3124, 3214
PsyI GACNNNGTC 1 cut(s) 3095
PvuII CAGCTG 1 cut(s) 1031
RruI TCGCGA 1 cut(s) 2487
RsaI GTAC 5 cut(s) 1091, 2139, 2451, 2501, 3035
RsaNI GTAC 5 cut(s) 1090, 2138, 2450, 2500, 3034
RseI CAYNNNNRTG 5 cut(s) 786, 1503, 2297, 2405, 3411
SacI GAGCTC 1 cut(s) 475
SalI GTCGAC 1 cut(s) 200
SapI GCTCTTC 4 cut(s) 480, 1010, 2238, 2390
Sau96I GGNCC 6 cut(s) 837, 1792, 3754, 4005, 4015, 4055
ScaI AGTACT 1 cut(s) 1091
SchI GAGTC 6 cut(s) 512, 1322, 1972, 2388, 3326, 3749
ScrFI CCNGG 7 cut(s) 297, 468, 864, 1685, 3028, 3914, 4019
SduI GDGCHC 3 cut(s) 475, 1378, 1699
SinI GGWCC 5 cut(s) 837, 3754, 4005, 4015, 4055
SmiMI CAYNNNNRTG 5 cut(s) 786, 1503, 2297, 2405, 3411
SmlI CTYRAG 4 cut(s) 167, 324, 1093, 2706
SmoI CTYRAG 4 cut(s) 167, 324, 1093, 2706
SnaBI TACGTA 2 cut(s) 2867, 3650
SpeI ACTAGT 1 cut(s) 941
SphI GCATGC 1 cut(s) 785
SseBI AGGCCT 3 cut(s) 2821, 3373, 3712
SsiI CCGC 7 cut(s) 1479, 1663, 1948, 2104, 3792, 3893, 3971
SspI AATATT 3 cut(s) 1216, 2446, 2934
SspMI CTAG 9 cut(s) 942, 2124, 2817, 3131, 3164, 3329, 3342, 3795, 3854
SstI GAGCTC 1 cut(s) 475
StuI AGGCCT 3 cut(s) 2821, 3373, 3712
StyD4I CCNGG 7 cut(s) 295, 466, 862, 1683, 3026, 3912, 4017
StyI CCWWGG 6 cut(s) 283, 480, 745, 1795, 2816, 2872
TaaI ACNGT 8 cut(s) 1170, 1187, 1768, 1979, 2999, 3097, 3645, 3777
TaiI ACGT 5 cut(s) 314, 2702, 2869, 3432, 3652
TaqI TCGA 4 cut(s) 201, 567, 3512, 4069
TatI WGTACW 3 cut(s) 1089, 2137, 2449
TauI GCSGC 3 cut(s) 1481, 1950, 3794
TseFI GTSAC 7 cut(s) 1513, 1594, 1879, 2155, 2700, 2876, 4031
Tsp45I GTSAC 7 cut(s) 1513, 1594, 1879, 2155, 2700, 2876, 4031
TspGWI ACGGA 6 cut(s) 964, 1214, 1975, 2487, 3321, 3883
Tth111I GACNNNGTC 1 cut(s) 3095
Van91I CCANNNNNTGG 2 cut(s) 196, 3751
VpaK11BI GGWCC 5 cut(s) 837, 3754, 4005, 4015, 4055
VspI ATTAAT 2 cut(s) 2333, 3524
XagI CCTNNNNNAGG 3 cut(s) 633, 1688, 3761
XapI RAATTY 2 cut(s) 1864, 2228
XceI RCATGY 6 cut(s) 785, 1656, 2138, 2404, 3045, 4039
XcmI CCANNNNNNNNNTGG 3 cut(s) 3164, 3413, 3925
XmaJI CCTAGG 1 cut(s) 2816
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 2 cut(s) 1452, 1674
XspI CTAG 9 cut(s) 942, 2124, 2817, 3131, 3164, 3329, 3342, 3795, 3854
ZrmI AGTACT 1 cut(s) 1091
Zsp2I ATGCAT 3 cut(s) 712, 783, 2680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.