MD02G1202100.v1.1

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007735
Physical Location & Seq
Reverse (-)
20019074 .. 20025652
6579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1202100.v1.1.491

Sequence Viewer

Length: 4182 bp
ATGATGAATTTTGTGATGTGGAACAGGTTTTTAGGTGCGATTAAGCAATATTTATGTCTGTCACTGTTAAAGAACAGTGCTTCAACTCTTATGATAGTTTTTCAGCTATCTTGCTCCATCTTCATTAGTCTGGTGTCAAGATTTAGAGCTGGACTGAAAGCAGAGATTGGAGTATTTTTTCCCATGATTGTTCTCAGAGTTTTGGAAAATGTCGCACAACCCAATTTTCAGCAAAAGATGATAGTACTTCGGTTTCTTGAGAAGCTGTGTGTTGATTCCCAGATCTTGGTAGACATATTTATCAATTATGATTGTGATGTCAATTCATCGAACATATTTGAAAGGATGGTAAATGGCCTACTTAAAACTGCTCAAGGTGTCCCTCCTGGTGTTGCCACCACATTATTACCACCTCAGGAGGCAACTATGAAACTTGAAGCTATGAAGTGCTTAGTTGGTGTTTTGAGGTCAATTGGAGATTGGATGAACAAACAATTGCGCATTCCAGATCCTCATTCTATCAAGAGATTTGAGCCAACTGAGAATAGTCCTGAACCAGGAGGTCTTCCCTTGGCAAATGGGAACAGCGAGGAGCCTGTTGATGGGTCAGACACTCATTCTGAAGCCTCCAGTGAGGCCTCTGATGCTTTTTTGACAATTGAACAACGCCGAGCTTACAAGCTGGAACTTCAGGAAGGTATATCTCTTTTTAATCGGAAACCTAAAAAAGGAATTGAGTTTCTTATTAATGCAAACAAGGTGGGTAGTTCACCTGAGGAGATAGCTGCTTTTCTCAAAAATGCATCTGGTTTGAACAAGACTTTGATTGGGGATTACCTTGGAGAAAGAGAAGATTTATCGCTAAAAGTAATGCATGCTTATGTGGATTCATTTGAATTTCAAGGCTTGGAGTTTGATGAGGCCATCAGGGCATTTCTTCAAGGCTTTAGGTTGCCTGGTGAGGCACAGAAGATCGATAGAATAATGGAAAAGTTTGCAGAACGCTATTGCAAGTGTAATCCGAAGGCTTTTACTAGTGCTGACACAGCCTATGTTCTTGCTTATTCCGTGATATTGCTCAACACTGATGCTCATAACCCTATGGTGAAGAACAAGATGTCAGCAGATGATTTTATAAGAAACAATCGCGGCATAGATGATGGAAAAGATCTACCTGAGGAGTACTTGAGGTCATTGTTTGAACGAATATCAAGAAATGAGATTAAAATGAAAGAATATGAATTGGCTCCTCAAATACAGTCGGTGAACCCAAACCGGCTTTTGGGCTTGGACAGTATCTTGAATATTGTGATCCGTAAACGGGGGGAAGGAAATCAATTGGAGACCAGTGATGATCTTATCAAACATATGCAAGAACAATTTAAAGAAAAAGCTCGCAAATCTGAGTCAGTTTATTATGCTGCAACAGATGTGATAATTCTTAGATTCATGGTTGAGGTATGCTGGGCCCCTATGCTGGCTGCCTTCAGTGTTCCACTTGATCAAAGTGACGATGAAGTAGTTATTTCACTGTGTCTGGAAGGCTTTCGTCATGCCATCCATGTTACTGCTGTAATGTCCATGAAGACCCATAGAGACGCTTTTGTGACTTCACTAGCGAAGTTTACTTCTCTCCACTCTCCTGCCGATATCAAGCAGAAGAATATTGATGCAATTAAGGCAATAGTGACAATTGCAGATGAAGATGGAAACTACCTACAAGAAGCTTGGGAACATATTTTGACTTGTGTTTCCCGGTTTGAACATCTACATCTCTTGGGAGAGGGTGCTCCTCCAGATGCTACATTCTTTGCCTTTCCTCAGAATGAGTCAGAAAAAGCAAAACAGACTAAGTCCACTATCCTTCCTGTTTTAAAAAAGAAGGGGCAAGGAAGGATGCAGTATGCAGCTGCTGCTGTGTTGCGAGGTTCATATGATAGTGCTGGTATTGGTGGGAATGCTTCTGGGGCAGTCACATCAGAACAGATGAACAATTTGGTTTCTAATTTAAACATGCTAGAACAAGTTGGTGAAATGAGCCGCATATTCACACGTAGTCAGAAACTAAACAGTGAGGCGATTGTGGACTTTGTTAAGGCACTTTGCAAGGTCTCTATGGAAGAATTGCGATCAGCATCTGATCCTCGGGTTTTCAGCCTTACAAAGATTGTTGAGATTGCGCATTATAATATGAACCGCATCAGGCTTGTCTGGTCTAGCATCTGGCATGTGCTCTCTAATTTCTTTGTGACAATTGGCTGTTCTGAAAACCTGTCAATTGCCATTTTTGCTATGGATTCGCTACGCCAGTTGTCAATGAAGTTCTTGGACCGAGAAGAGTTGGCTAATTATAATTTTCAGAATGAGTTCATGAAACCTTTTGTCATTGTTATGCGTAAAAGCAGTGCAGTTGAAATACGTGAATTAATCATCAGATGTGTCTCACAAATGGTATTATCTCGTGTCAACAATGTCAAATCTGGATGGAAGAGCATGTTCATGGTGTTCACTACTGCAGCTTATGACGACCACAAAAACATTGTACTCTTAGCCTTTGAAATAATTGAGAAGATTATTCGAGACTATTTTCCGTACATTACTGAGACTGAAACCACTACCTTTACTGATTGCGTGAATTGCCTAATTGCATTTACCAATAGTAGATTCAACAAGGACATTAGCCTCAATGCTATTGCTTTTCTTCGATTCTGTGCTACAAAACTTGCAGATGGAGGTCTTGGTTCCTCGTCAAAAAATAAGGACAAGGAAGCTTCTGGAAAAATCTCTCCATCTTCACCTCAAGCCTGGAAAGATGGAAAACAAGAAAATGGTGAAATGCCAGACAAGGATGACCATATCTATTTCTGGTTCCCTTTATTGGCTGGTTTATCTGAGCTTAGCTTTGATCCTAGGCCTGAAATCAGGAGGAGTGCCCTACAAGTTCTGTTTGAAACTTTACGTAACCATGGTCATCTTTTCTCACTACCTTTGTGGGAAAGGGTGTTTGAATCAGTGCTATTTCCAATATTTGACTATGTGCGGCATGCTATCGATCCATCTGGGGAAGGTTCACCAGGGCAAGGGACTGATGGTGATGTGGGTGACCTTGATCAAGATGCCTGGCTCTATGAGACATGCACATTGGCACTTCAATTAGTTGTTGATCTCTTTGTCAAATTTTATAACACTGTCAATCCGCTATTGAAGAAAGTTTTGGTGCTTCTAGTCAGTTTTATTCGACGACCACACCAAAGCTTAGCTGGTATTGGTATTGCTGCATTTGTGCGATTGATGAGCAATGCAGGGGATCTCTTTTCTCATGAAAAGTGGCTAGAAGTGGTTTCATCATTAAAAGAAGCAGCCGATTCAACACTTCCTGATTTCTCTTTCCTTCTTAGTGGAGATGGCATCATTAGAAGCCATGAACATGCTTTGAGCAGAGAAGAGAATGGAGAGTCCACCGTGTCTGGCAGGAGTGATGAGGATTCAGAGAGACTGAGGACAAACCACATGTATGCTGGAATATCTGACGTCAAGTGCCGAGCTGCTGTTCAACTTTTATTGATTCAGGCGGTGATGGAGATTTACACCATGTATCGCTCTCACCTTTCCGCGAGAAACACATTAGTCCTTTTTGATGCTTTGCATGATGTTGCATCTCACGCTCACAAGATTAACACCGATACCACTTTAAGAGCAAGGTTACAAGAATTTGGCTCCGTGACCCAAATGCAAGACCCTCCACTGTTGCGCATCGAGAACGAATCTTACCAAATATGCCTTACATTCTTACAGAACCTTGTAGAGGATAGCCCAGCAGGTTACGATGAAGCGGAGGTGGAGTCCTACATCGTGGACCTCTGCAGAGAGGTTTTACAGTTCTATATTGAAGCTGCTAGCTCTGGAAAGGTATCTGAATCATCCAAAGGGCAGCAGCTCCATTGGCTAATTCCTCTAGGTTCTGGGAGACGAAGAGAATTGGCCCAACGTGCCCCGCTTATTGTGGCAACTCTCCAGACTATCTGTAGTTTGGGAGAGACCTCATTTGAAAACAACTTGTCTCAGTTCTTCCCCCTCCTTTCCAGCTTGATAAGTTGTGAACACGGTTCAAATGAGGTCCAGATTGCCCTCGGTGACATGCTAAGCTCATCTGTGGGGCCTGTGTTGCTTCGGTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1394

Amino Acids

156.34

Weight (kDa)

5.62

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 6 - 112 5.9e-31 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 227 - 408 8.4e-78 Sec7 domain
Sec7-like_HDS PF09324 774 - 856 3.5e-30 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 945 - 1016 2.1e-11 C-terminal region of Mon2 protein
BIG2_C PF20252 1183 - 1382 2.4e-42 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1136, 2187, 2352, 3183
AatII GACGTC 1 cut(s) 3534
Acc16I TGCGCA 3 cut(s) 500, 2181, 3752
Acc36I ACCTGC 1 cut(s) 3809
AccB7I CCANNNNNTGG 1 cut(s) 286
AccI GTMKAC 1 cut(s) 291
AccII CGCG 2 cut(s) 1149, 3614
AciI CCGC 9 cut(s) 1149, 2041, 2197, 3040, 3197, 3572, 3612, 3833, 3995
AclWI GGATC 6 cut(s) 503, 1306, 2135, 2900, 3047, 3315
AcsI RAATTY 4 cut(s) 7, 896, 3176, 3710
AcuI CTGAAG 3 cut(s) 642, 674, 1471
AcyI GRCGYC 1 cut(s) 3531
AfaI GTAC 4 cut(s) 246, 1184, 2544, 2594
AflIII ACRYGT 2 cut(s) 2051, 3510
AhlI ACTAGT 1 cut(s) 1034
AjnI CCWGG 6 cut(s) 385, 556, 955, 2804, 3073, 3119
AjuI GAANNNNNNNTTGG 4 cut(s) 2648, 2680, 3197, 3229
Alw21I GWGCWC 2 cut(s) 1792, 2235
AlwI GGATC 6 cut(s) 503, 1306, 2135, 2900, 3047, 3315
AlwNI CAGNNNCTG 2 cut(s) 1913, 2138
Ama87I CYCGRG 1 cut(s) 2145
AoxI GGCC 7 cut(s) 355, 636, 921, 1467, 2912, 3981, 4157
ApaI GGGCCC 1 cut(s) 1471
ApoI RAATTY 4 cut(s) 7, 896, 3176, 3710
AseI ATTAAT 2 cut(s) 747, 2426
Asp700I GAANNNNTTC 2 cut(s) 1545, 2366
AspA2I CCTAGG 1 cut(s) 2909
AspLEI GCGC 3 cut(s) 501, 2182, 3753
AspS9I GGNCC 7 cut(s) 1467, 1468, 2329, 3856, 3982, 4117, 4157
AsuC2I CCSGG 1 cut(s) 1756
AsuNHI GCTAGC 1 cut(s) 3896
AvaI CYCGRG 1 cut(s) 2145
AvaII GGWCC 3 cut(s) 2329, 3856, 4117
AvrII CCTAGG 1 cut(s) 2909
AxyI CCTNAGG 3 cut(s) 414, 774, 1176
BaeGI GKGCMC 3 cut(s) 1471, 2935, 3994
BanII GRGCYC 1 cut(s) 1471
BauI CACGAG 1 cut(s) 2460
BbsI GAAGAC 2 cut(s) 557, 1592
Bbv12I GWGCWC 2 cut(s) 1792, 2235
BciT130I CCWGG 6 cut(s) 387, 558, 957, 2806, 3075, 3121
BclI TGATCA 2 cut(s) 1501, 3109
BcnI CCSGG 1 cut(s) 1756
BcuI ACTAGT 1 cut(s) 1034
BfaI CTAG 9 cut(s) 1035, 1616, 2018, 2217, 2910, 3224, 3332, 3897, 3956
BfmI CTRYAG 3 cut(s) 2514, 3862, 4024
BfuAI ACCTGC 1 cut(s) 3809
BglI GCCNNNNNGGC 1 cut(s) 929
BglII AGATCT 2 cut(s) 282, 1168
BlnI CCTAGG 1 cut(s) 2909
BlpI GCTNAGC 3 cut(s) 2897, 3256, 4142
BmcAI AGTACT 2 cut(s) 246, 1184
Bme1390I CCNGG 7 cut(s) 387, 558, 957, 1756, 2806, 3075, 3121
Bme18I GGWCC 3 cut(s) 2329, 3856, 4117
BmeT110I CYCGRG 1 cut(s) 2145
BmgT120I GGNCC 7 cut(s) 1467, 1468, 2329, 3856, 3982, 4117, 4157
BmiI GGNNCC 8 cut(s) 594, 1248, 1469, 1470, 2743, 2870, 3718, 4158
BmrFI CCNGG 7 cut(s) 387, 558, 957, 1756, 2806, 3075, 3121
BmtI GCTAGC 1 cut(s) 3900
BpiI GAAGAC 2 cut(s) 557, 1592
BpmI CTGGAG 3 cut(s) 613, 1779, 3998
Bpu1102I GCTNAGC 3 cut(s) 2897, 3256, 4142
BpuEI CTTGAG 4 cut(s) 278, 357, 1207, 2784
BpuMI CCSGG 1 cut(s) 1756
Bsa29I ATCGAT 2 cut(s) 975, 3051
BsaAI YACGTR 3 cut(s) 2054, 2420, 2960
BsaBI GATNNNNATC 1 cut(s) 2134
BsaHI GRCGYC 1 cut(s) 3531
BsaI GGTCTC 3 cut(s) 1337, 2116, 4031
BsaJI CCNNGG 7 cut(s) 570, 838, 2144, 2909, 2965, 3074, 4129
BsaXI ACNNNNNCTCC 2 cut(s) 3827, 3857
Bse118I RCCGGY 1 cut(s) 1275
Bse1I ACTGG 3 cut(s) 630, 1347, 2308
Bse21I CCTNAGG 3 cut(s) 414, 774, 1176
Bse3DI GCAATG 1 cut(s) 3304
Bse8I GATNNNNATC 1 cut(s) 2134
BseBI CCWGG 6 cut(s) 387, 558, 957, 2806, 3075, 3121
BseCI ATCGAT 2 cut(s) 975, 3051
BseDI CCNNGG 7 cut(s) 570, 838, 2144, 2909, 2965, 3074, 4129
BseGI GGATG 7 cut(s) 351, 489, 1557, 1902, 2489, 2854, 3920
BseJI GATNNNNATC 1 cut(s) 2134
BseMI GCAATG 1 cut(s) 3304
BseNI ACTGG 3 cut(s) 630, 1347, 2308
BseRI GAGGAG 6 cut(s) 605, 791, 1193, 1239, 1782, 2941
BseSI GKGCMC 3 cut(s) 1471, 2935, 3994
BseYI CCCAGC 2 cut(s) 1464, 3814
BsgI GTGCAG 1 cut(s) 2427
Bsh1236I CGCG 2 cut(s) 1149, 3614
Bsh1285I CGRYCG 1 cut(s) 4175
BshFI GGCC 7 cut(s) 357, 638, 923, 1469, 2914, 3983, 4159
BshVI ATCGAT 2 cut(s) 975, 3051
BsiEI CGRYCG 1 cut(s) 4175
BsiHKAI GWGCWC 2 cut(s) 1792, 2235
BsiHKCI CYCGRG 1 cut(s) 2145
BsiSI CCGG 2 cut(s) 1276, 1756
BslFI GGGAC 2 cut(s) 365, 3097
BsmBI CGTCTC 2 cut(s) 1590, 3961
BsmFI GGGAC 2 cut(s) 365, 3097
BsmI GAATGC 2 cut(s) 501, 1963
BsnI GGCC 7 cut(s) 357, 638, 923, 1469, 2914, 3983, 4159
Bso31I GGTCTC 3 cut(s) 1337, 2116, 4031
BsoBI CYCGRG 1 cut(s) 2145
Bsp120I GGGCCC 1 cut(s) 1467
Bsp1286I GDGCHC 5 cut(s) 1471, 1792, 2235, 2935, 3994
Bsp1720I GCTNAGC 3 cut(s) 2897, 3256, 4142
Bsp19I CCATGG 1 cut(s) 2965
BspACI CCGC 9 cut(s) 1149, 2041, 2197, 3040, 3197, 3572, 3612, 3833, 3995
BspANI GGCC 7 cut(s) 357, 638, 923, 1469, 2914, 3983, 4159
BspDI ATCGAT 2 cut(s) 975, 3051
BspFNI CGCG 2 cut(s) 1149, 3614
BspHI TCATGA 2 cut(s) 2370, 3319
BspLI GGNNCC 8 cut(s) 594, 1248, 1469, 1470, 2743, 2870, 3718, 4158
BspMAI CTGCAG 2 cut(s) 2518, 3866
BspMI ACCTGC 1 cut(s) 3809
BspOI GCTAGC 1 cut(s) 3900
BspPI GGATC 6 cut(s) 503, 1306, 2135, 2900, 3047, 3315
BspQI GCTCTTC 1 cut(s) 2483
BspTNI GGTCTC 3 cut(s) 1337, 2116, 4031
BsrDI GCAATG 1 cut(s) 3304
BsrFI RCCGGY 1 cut(s) 1275
BsrI ACTGG 3 cut(s) 630, 1347, 2308
BssAI RCCGGY 1 cut(s) 1275
BssECI CCNNGG 7 cut(s) 570, 838, 2144, 2909, 2965, 3074, 4129
BssNI GRCGYC 1 cut(s) 3531
BssSI CACGAG 1 cut(s) 2460
BssT1I CCWWGG 4 cut(s) 570, 838, 2909, 2965
Bst2BI CACGAG 1 cut(s) 2460
Bst2UI CCWGG 6 cut(s) 387, 558, 957, 2806, 3075, 3121
Bst6I CTCTTC 4 cut(s) 2331, 2483, 3438, 3967
BstACI GRCGYC 1 cut(s) 3531
BstAPI GCANNNNNTGC 1 cut(s) 1913
BstBAI YACGTR 3 cut(s) 2054, 2420, 2960
BstC8I GCNNGC 5 cut(s) 876, 1396, 1479, 3045, 3898
BstDSI CCRYGG 1 cut(s) 2965
BstEII GGTNACC 1 cut(s) 3101
BstENI CCTNNNNNAGG 3 cut(s) 555, 726, 3803
BstF5I GGATG 7 cut(s) 351, 489, 1557, 1902, 2489, 2854, 3920
BstFNI CGCG 2 cut(s) 1149, 3614
BstHHI GCGC 3 cut(s) 501, 2182, 3753
BstMCI CGRYCG 1 cut(s) 4175
BstNI CCWGG 6 cut(s) 387, 558, 957, 2806, 3075, 3121
BstNSI RCATGY 9 cut(s) 878, 2017, 2231, 2497, 3047, 3138, 3431, 3514, 4141
BstPI GGTNACC 1 cut(s) 3101
BstSCI CCNGG 7 cut(s) 385, 556, 955, 1754, 2804, 3073, 3119
BstSFI CTRYAG 3 cut(s) 2514, 3862, 4024
BstSLI GKGCMC 3 cut(s) 1471, 2935, 3994
BstSNI TACGTA 1 cut(s) 2960
BstUI CGCG 2 cut(s) 1149, 3614
BstV2I GAAGAC 2 cut(s) 557, 1592
BstX2I RGATCY 4 cut(s) 282, 508, 1168, 3307
BstYI RGATCY 4 cut(s) 282, 508, 1168, 3307
Bsu15I ATCGAT 2 cut(s) 975, 3051
Bsu36I CCTNAGG 3 cut(s) 414, 774, 1176
BsuRI GGCC 7 cut(s) 357, 638, 923, 1469, 2914, 3983, 4159
BsuTUI ATCGAT 2 cut(s) 975, 3051
BtgI CCRYGG 1 cut(s) 2965
BtsCI GGATG 7 cut(s) 351, 489, 1557, 1902, 2489, 2854, 3920
BtsI GCAGTG 1 cut(s) 2410
BveI ACCTGC 1 cut(s) 3809
Cac8I GCNNGC 5 cut(s) 876, 1396, 1479, 3045, 3898
CaiI CAGNNNCTG 2 cut(s) 1913, 2138
CciI TCATGA 2 cut(s) 2370, 3319
CfoI GCGC 3 cut(s) 501, 2182, 3753
Cfr10I RCCGGY 1 cut(s) 1275
Cfr13I GGNCC 7 cut(s) 1467, 1468, 2329, 3856, 3982, 4117, 4157
ClaI ATCGAT 2 cut(s) 975, 3051
CseI GACGC 1 cut(s) 1607
Csp6I GTAC 4 cut(s) 245, 1183, 2543, 2593
CspCI CAANNNNNGTGG 2 cut(s) 741, 776
CviQI GTAC 4 cut(s) 245, 1183, 2543, 2593
DraI TTTAAA 3 cut(s) 1384, 1875, 2010
Eam1104I CTCTTC 4 cut(s) 2331, 2483, 3438, 3967
EarI CTCTTC 4 cut(s) 2331, 2483, 3438, 3967
Eco105I TACGTA 1 cut(s) 2960
Eco130I CCWWGG 4 cut(s) 570, 838, 2909, 2965
Eco147I AGGCCT 2 cut(s) 638, 2914
Eco24I GRGCYC 1 cut(s) 1471
Eco31I GGTCTC 3 cut(s) 1337, 2116, 4031
Eco32I GATATC 1 cut(s) 1651
Eco47I GGWCC 3 cut(s) 2329, 3856, 4117
Eco57I CTGAAG 3 cut(s) 642, 674, 1471
Eco81I CCTNAGG 3 cut(s) 414, 774, 1176
Eco88I CYCGRG 1 cut(s) 2145
Eco91I GGTNACC 1 cut(s) 3101
EcoNI CCTNNNNNAGG 3 cut(s) 555, 726, 3803
EcoO109I RGGNCCY 2 cut(s) 1468, 4157
EcoO65I GGTNACC 1 cut(s) 3101
EcoRII CCWGG 6 cut(s) 385, 556, 955, 2804, 3073, 3119
EcoRV GATATC 1 cut(s) 1651
EcoT14I CCWWGG 4 cut(s) 570, 838, 2909, 2965
EcoT22I ATGCAT 2 cut(s) 805, 876
EcoT38I GRGCYC 1 cut(s) 1471
ErhI CCWWGG 4 cut(s) 570, 838, 2909, 2965
Esp3I CGTCTC 2 cut(s) 1590, 3961
FaqI GGGAC 2 cut(s) 365, 3097
FauI CCCGC 1 cut(s) 4002
FauNDI CATATG 2 cut(s) 1368, 1933
FbaI TGATCA 2 cut(s) 1501, 3109
FblI GTMKAC 1 cut(s) 291
FokI GGATG 7 cut(s) 358, 496, 1544, 1909, 2496, 2861, 3907
FriOI GRGCYC 1 cut(s) 1471
FspBI CTAG 9 cut(s) 1035, 1616, 2018, 2217, 2910, 3224, 3332, 3897, 3956
FspI TGCGCA 3 cut(s) 500, 2181, 3752
GlaI GCGC 3 cut(s) 500, 2181, 3752
GsaI CCCAGC 2 cut(s) 1468, 3818
GsuI CTGGAG 3 cut(s) 613, 1779, 3998
HaeIII GGCC 7 cut(s) 357, 638, 923, 1469, 2914, 3983, 4159
HapII CCGG 2 cut(s) 1276, 1756
HgaI GACGC 1 cut(s) 1607
HhaI GCGC 3 cut(s) 501, 2182, 3753
Hin1I GRCGYC 1 cut(s) 3531
Hin6I GCGC 3 cut(s) 499, 2180, 3751
HinP1I GCGC 3 cut(s) 499, 2180, 3751
HincII GTYRAC 1 cut(s) 2467
HindII GTYRAC 1 cut(s) 2467
HindIII AAGCTT 3 cut(s) 1725, 2769, 3253
HpaII CCGG 2 cut(s) 1276, 1756
Hpy99I CGWCG 1 cut(s) 3243
HpyCH4IV ACGT 5 cut(s) 2053, 2419, 2959, 3531, 3988
HpySE526I ACGT 5 cut(s) 2053, 2419, 2959, 3531, 3988
Hsp92I GRCGYC 1 cut(s) 3531
HspAI GCGC 3 cut(s) 499, 2180, 3751
Ksp22I TGATCA 2 cut(s) 1501, 3109
LguI GCTCTTC 1 cut(s) 2483
LmnI GCTCC 6 cut(s) 119, 592, 1252, 1795, 3722, 3942
MaeI CTAG 9 cut(s) 1035, 1616, 2018, 2217, 2910, 3224, 3332, 3897, 3956
MaeII ACGT 5 cut(s) 2053, 2419, 2959, 3531, 3988
MfeI CAATTG 7 cut(s) 471, 494, 657, 1337, 1692, 2253, 2277
MflI RGATCY 4 cut(s) 282, 508, 1168, 3307
MhlI GDGCHC 5 cut(s) 1471, 1792, 2235, 2935, 3994
MlyI GAGTC 4 cut(s) 1415, 1838, 3464, 3851
Mph1103I ATGCAT 2 cut(s) 805, 876
MroXI GAANNNNTTC 2 cut(s) 1545, 2366
MslI CAYNNNNRTG 5 cut(s) 879, 2390, 2498, 3426, 3513
MspA1I CMGCKG 1 cut(s) 1910
MspI CCGG 2 cut(s) 1276, 1756
MspR9I CCNGG 7 cut(s) 387, 558, 957, 1756, 2806, 3075, 3121
MunI CAATTG 7 cut(s) 471, 494, 657, 1337, 1692, 2253, 2277
Mva1269I GAATGC 2 cut(s) 501, 1963
MvaI CCWGG 6 cut(s) 387, 558, 957, 2806, 3075, 3121
MvnI CGCG 2 cut(s) 1149, 3614
NciI CCSGG 1 cut(s) 1756
NcoI CCATGG 1 cut(s) 2965
NdeI CATATG 2 cut(s) 1368, 1933
NheI GCTAGC 1 cut(s) 3896
NlaIV GGNNCC 8 cut(s) 594, 1248, 1469, 1470, 2743, 2870, 3718, 4158
NmeAIII GCCGAG 2 cut(s) 695, 3566
NmuCI GTSAC 9 cut(s) 60, 1508, 1606, 1687, 1972, 2248, 3101, 3721, 4133
NsbI TGCGCA 3 cut(s) 500, 2181, 3752
NsiI ATGCAT 2 cut(s) 805, 876
NspI RCATGY 9 cut(s) 878, 2017, 2231, 2497, 3047, 3138, 3431, 3514, 4141
PaeI GCATGC 2 cut(s) 878, 3047
PagI TCATGA 2 cut(s) 2370, 3319
PceI AGGCCT 2 cut(s) 638, 2914
PciI ACATGT 1 cut(s) 3510
PciSI GCTCTTC 1 cut(s) 2483
PctI GAATGC 2 cut(s) 501, 1963
PdmI GAANNNNTTC 2 cut(s) 1545, 2366
PflFI GACNNNGTC 1 cut(s) 1852
PflMI CCANNNNNTGG 1 cut(s) 286
PleI GAGTC 4 cut(s) 1414, 1837, 3463, 3850
PpsI GAGTC 4 cut(s) 1414, 1837, 3463, 3850
Ppu21I YACGTR 3 cut(s) 2054, 2420, 2960
PscI ACATGT 1 cut(s) 3510
PshBI ATTAAT 2 cut(s) 747, 2426
PsiI TTATAA 4 cut(s) 1136, 2187, 2352, 3183
Psp6I CCWGG 6 cut(s) 385, 556, 955, 2804, 3073, 3119
PspEI GGTNACC 1 cut(s) 3101
PspFI CCCAGC 2 cut(s) 1464, 3814
PspGI CCWGG 6 cut(s) 385, 556, 955, 2804, 3073, 3119
PspN4I GGNNCC 8 cut(s) 594, 1248, 1469, 1470, 2743, 2870, 3718, 4158
PspOMI GGGCCC 1 cut(s) 1467
PspPI GGNCC 7 cut(s) 1467, 1468, 2329, 3856, 3982, 4117, 4157
PsrI GAACNNNNNNTAC 2 cut(s) 3752, 3784
PstI CTGCAG 2 cut(s) 2518, 3866
PstNI CAGNNNCTG 2 cut(s) 1913, 2138
PsuI RGATCY 4 cut(s) 282, 508, 1168, 3307
PsyI GACNNNGTC 1 cut(s) 1852
PvuII CAGCTG 1 cut(s) 1910
RsaI GTAC 4 cut(s) 246, 1184, 2544, 2594
RsaNI GTAC 4 cut(s) 245, 1183, 2543, 2593
RseI CAYNNNNRTG 5 cut(s) 879, 2390, 2498, 3426, 3513
SapI GCTCTTC 1 cut(s) 2483
Sau96I GGNCC 7 cut(s) 1467, 1468, 2329, 3856, 3982, 4117, 4157
ScaI AGTACT 2 cut(s) 246, 1184
SchI GAGTC 4 cut(s) 1415, 1838, 3464, 3851
ScrFI CCNGG 7 cut(s) 387, 558, 957, 1756, 2806, 3075, 3121
SduI GDGCHC 5 cut(s) 1471, 1792, 2235, 2935, 3994
SfcI CTRYAG 3 cut(s) 2514, 3862, 4024
SinI GGWCC 3 cut(s) 2329, 3856, 4117
SmiMI CAYNNNNRTG 5 cut(s) 879, 2390, 2498, 3426, 3513
SmlI CTYRAG 4 cut(s) 257, 372, 1186, 2799
SmoI CTYRAG 4 cut(s) 257, 372, 1186, 2799
SnaBI TACGTA 1 cut(s) 2960
SpeI ACTAGT 1 cut(s) 1034
SphI GCATGC 2 cut(s) 878, 3047
SseBI AGGCCT 2 cut(s) 638, 2914
SsiI CCGC 9 cut(s) 1149, 2041, 2197, 3040, 3197, 3572, 3612, 3833, 3995
SspI AATATT 4 cut(s) 50, 1306, 1666, 3027
SspMI CTAG 9 cut(s) 1035, 1616, 2018, 2217, 2910, 3224, 3332, 3897, 3956
StuI AGGCCT 2 cut(s) 638, 2914
StyD4I CCNGG 7 cut(s) 385, 556, 955, 1754, 2804, 3073, 3119
StyI CCWWGG 4 cut(s) 570, 838, 2909, 2965
TaiI ACGT 5 cut(s) 2056, 2422, 2962, 3534, 3991
TaqI TCGA 7 cut(s) 329, 975, 2578, 2704, 3051, 3238, 3756
TaqII GACCGA 2 cut(s) 2346, 4161
TatI WGTACW 3 cut(s) 244, 1182, 2542
TauI GCSGC 3 cut(s) 1152, 2043, 3043
TseFI GTSAC 9 cut(s) 60, 1508, 1606, 1687, 1972, 2248, 3101, 3721, 4133
Tsp45I GTSAC 9 cut(s) 60, 1508, 1606, 1687, 1972, 2248, 3101, 3721, 4133
TspGWI ACGGA 4 cut(s) 1057, 1304, 2580, 3709
Tth111I GACNNNGTC 1 cut(s) 1852
Van91I CCANNNNNTGG 1 cut(s) 286
VpaK11BI GGWCC 3 cut(s) 2329, 3856, 4117
VspI ATTAAT 2 cut(s) 747, 2426
XagI CCTNNNNNAGG 3 cut(s) 555, 726, 3803
XapI RAATTY 4 cut(s) 7, 896, 3176, 3710
XceI RCATGY 9 cut(s) 878, 2017, 2231, 2497, 3047, 3138, 3431, 3514, 4141
XcmI CCANNNNNNNNNTGG 3 cut(s) 2290, 3257, 3515
XmaJI CCTAGG 1 cut(s) 2909
XmiI GTMKAC 1 cut(s) 291
XmnI GAANNNNTTC 2 cut(s) 1545, 2366
XspI CTAG 9 cut(s) 1035, 1616, 2018, 2217, 2910, 3224, 3332, 3897, 3956
ZraI GACGTC 1 cut(s) 3532
ZrmI AGTACT 2 cut(s) 246, 1184
Zsp2I ATGCAT 2 cut(s) 805, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.