Rroxscaffold_4G00304550

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007735
Physical Location & Seq
Reverse (-)
25202830 .. 25211853
9024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304550.1

Sequence Viewer

Length: 2817 bp
ATGGCTTCTTCGGAAGCCGATTCCCGCCTCCGACAGGTCGTTTCTCCAGCCCTAGAGAAGATCATCAAGAACGCCTCCTGGCGCAAGCACGCCAAGCTCGCCAACGAATGCAAGGCCGTCATCGAGCGCCTCTCCAACCCCGACAAGGCGGCCCAGCCCGCCGGCCCGGATTCCGAACCCGACGCCTCCGAGCCCGGCCCGCTCCACGACGGAGGCTCCGGTGAGTACTCCCTCGCCGACGCCGAGTCGATTCTCAGCCCCCTCATCGGCGCCGCCGCATCCGGAGTGCTGAAGATCGCCGATCCCGCCGTCGATTGCATCCAGAAGCTCATCGCGCATGGCTACTTGCGCGGCGAGGCCGACGCCTCCGGTGGCGCCGAGGCGAAGTTGCTGACGAAATTGATCGAGTCCGTTTGTAAATGCCACGATTTGGGGGACGATCAGATGGAGCTGCTGGTGCTGAAGACGCTGCTGTCAGCAGTGACGTCGATTTCGCTGCGGATTCACGGCGATTGCTTGCTTCAGATAGTGAGGACTTGCTATGATATCTATTTGGGGAGTAAGAATATAGTGAATCAGACGACGGCGAAGGCCTCGCTGATCCAGATGCTGGTGATTGTGTTCCGGCGAATGGAGGCGGACTCTTCTACGGTGCCGATTAATCCCATTGTGGTGGCGGAGTTGATGGATCCCATTGAGAAATCTGATGCTGATGGCTCCATGACTATGTTCGTGCAAGGGTTTATTACCAAAATTATGTCTGACATTGATGGGGTGTTGAATCCGACGACGCCTACTAAGCTTTCCGGGCATGATGGGGCGTTCGAGACCACCACGGTGGAGACCACGAATCCGGCGGACTTGCTGGACTCGACTGATAAGGATATGTTGGATGCCAAGTACTGGGAGATTAGTATGTACAAGACGGCATTGGAGGGCAGAAAGGGAGAGCTGGCGGATGGGGAAGTAGAGAGAGATGAAGATTTGGAGGTTCAGATTGGTAATAAGTTGCGCAGAGATGCTTTTTTGGTGTTTAGAGCTCTTTGTAAGCTGTCTATGAAGACCCCGCCCAAGGAGGCATTGGCGGATCCGGAGTTGATGAAGGGAAAGATTGTGGCTTTGGAGCTTTTGAAGATTTTGTTGGAGAATGCTGGTGCTGTGTTTAGGACCAGCGACAGGTTTTTAGGTGCAATTAAGCAATACTTATGTCTGTCATTGTTAAAGAACAGTGCGTCAACTCTTATGATAGTTTTTCAGCTATCTTGTTCCATCTTCATTAGTCTGGTGTCAAGATTTAGAGCTGGATTGAAAGCAGAGATTGGAGTATTCTTTCCTATGATTGTTCTCAGAGTTTTAGAGAATGTTGCACAACCTAATTTTCAGCAGAAGATGATAGTGCTTCGGTTTCTTGAGAAGCTCTGTGTTGATTCCCAGATCTTGGTCGACATATTTATCAATTATGATTGCGATGTTAATTCATCAAACATATTTGAGAGGATGGTAAATGGCCTTCTTAAAACTGCCCAAGGCGTCCCTCCTGGTGTTGCCACCACGTTATTACCTCCTCAAGAGGCAAATATGAAACTTGAAGCCATGAGGTGTTTAGTTGCTATTCTGAGGTCAATCGGGGACTGGATGAACAAACAATTGCGCATTCCAGATCCTCATTCTACCAAGAAATCTGAAGTAAATGAGAATAGTTCTGAACCAGGAGCTCTTCCCATGGCAAATGGGAATGGTGAGGAGTCTGTTGAAGGATCAGATTCTCATTCTGAAGCCTCCAGTGAAGCCTCTGATGCTTTGACAATCGAGCAACGCCGAGCTTACAAACTGGAACTTCAGGAAGGTATATCTCTTTTCAATCGGAAACCTAAGAAAGGAATTGAGTTTCTTATCAATGCAAATAAGGTGGGCAATTCACCCGAGGAGATAGCTGCTTTTCTAAAAAATGCATCTGGTTTGAACAAGACTTTGATTGGAGACTACCTTGGAGAAAGAGAAGACTTATCGCTAAAAGTAATGCATGCTTATGTGGATTCCTTTGATTTTCAAGACTTGGAGTTTGATGAGGCAATCAGGTCCTTTCTTCAAGGCTTTAGGTTGCCTGGTGAAGCACAGAAGATTGACAGAATAATGGAAAAGTTTGCTGAGCGCTATTGCAAGTGCAATCCAAAGGCTTTTACTAGTGCTGATACAGCCTATGTCCTTGCTTACTCCGTGATACTGCTCAATACTGATGCGCATAACCCGATGGTGAAGAGCAAGATGTCAGCTGATGATTTTATAAGAAACAATCGTGGCATTGATGATGGCAAAGATCTACCTGAGCAGTACTTGAGGTCATTGTATGAACGAATATCAAGAAAGGAGATAAAAATGAAAGAATATGATTTGGCTCCTCAGCAGATACAGTCGGTGAACCCTAACCGTCTTTTGGGCTTGGATAGTATCTTGAATATTGTGATCCGTAAACGGGGGGATAGTCAATTGGAAACCAGTGATGATCTTATCAAGCATATGCAAGAGCAATTCCAAGAAAAAGCTCGGAAATCTGAGTCAGTTTACTATGCTGCAACAGATGTGGTAATTCTTAGATTCATGGTTGAAGTCTGCTGGGCTCCTATGCTGGCTGCCTTCAGTGTTCCACTTGACCAAACTGATGATGAAGTAGTTATTTCTCTGTGTCTTGAAGGGATTCGTTATGCTATCCATGTAACTGCCGCAATGTCCATGAAGACCCATAGAGATGCTTTTGTGACTTCACTGGCAAAGTTTACTTCCTCCACTCTCCTGCTGATATCAAGCAGAAAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

938

Amino Acids

103.67

Weight (kDa)

5.31

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 6 - 214 4.5e-38 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 338 - 496 7e-48 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 610 - 791 2.4e-78 Sec7 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2285
AasI GACNNNNNNGTC 1 cut(s) 472
AatII GACGTC 1 cut(s) 488
Acc16I TGCGCA 3 cut(s) 1013, 1652, 2241
AccB1I GGYRCC 3 cut(s) 269, 374, 652
AccB7I CCANNNNNTGG 4 cut(s) 430, 610, 903, 1438
AccBSI CCGCTC 1 cut(s) 202
AccI GTMKAC 1 cut(s) 1443
AccII CGCG 2 cut(s) 335, 351
AccIII TCCGGA 2 cut(s) 281, 1090
AclWI GGATC 9 cut(s) 296, 595, 683, 696, 1082, 1095, 1655, 1765, 2458
AcuI CTGAAG 7 cut(s) 311, 482, 506, 1704, 1794, 1823, 2620
AcyI GRCGYC 8 cut(s) 183, 240, 270, 363, 375, 485, 791, 1530
AfaI GTAC 4 cut(s) 227, 902, 920, 2333
AfeI AGCGCT 1 cut(s) 2153
AhdI GACNNNNNGTC 1 cut(s) 244
AhlI ACTAGT 1 cut(s) 2183
AjnI CCWGG 4 cut(s) 77, 1537, 1708, 2104
AjuI GAANNNNNNNTTGG 2 cut(s) 1124, 1156
AleI CACNNNNGTG 1 cut(s) 836
Alw21I GWGCWC 2 cut(s) 1042, 1717
Alw26I GTCTC 3 cut(s) 821, 836, 1974
AlwI GGATC 9 cut(s) 296, 595, 683, 696, 1082, 1095, 1655, 1765, 2458
AlwNI CAGNNNCTG 1 cut(s) 610
Ama87I CYCGRG 1 cut(s) 1922
Aor13HI TCCGGA 2 cut(s) 281, 1090
Aor51HI AGCGCT 1 cut(s) 2153
AoxI GGCC 7 cut(s) 114, 150, 163, 196, 357, 591, 1507
ApeKI GCWGC 6 cut(s) 451, 469, 496, 1934, 2570, 2630
ArsI GACNNNNNNTTYG 4 cut(s) 475, 507, 2045, 2077
AseI ATTAAT 1 cut(s) 660
Asp700I GAANNNNTTC 1 cut(s) 2694
AspS9I GGNCC 5 cut(s) 151, 164, 197, 1167, 2079
AsuC2I CCSGG 3 cut(s) 167, 195, 808
AsuHPI GGTGA 7 cut(s) 233, 625, 1751, 1911, 2120, 2266, 2428
AvaI CYCGRG 1 cut(s) 1922
AvaII GGWCC 2 cut(s) 1167, 2079
BamHI GGATCC 2 cut(s) 688, 1087
BanI GGYRCC 3 cut(s) 269, 374, 652
BanII GRGCYC 4 cut(s) 195, 1042, 1717, 2620
BarI GAAGNNNNNNTAC 2 cut(s) 1494, 1526
BbsI GAAGAC 4 cut(s) 470, 1067, 2007, 2741
Bbv12I GWGCWC 2 cut(s) 1042, 1717
BbvCI CCTCAGC 1 cut(s) 2400
BbvI GCAGC 6 cut(s) 438, 456, 483, 1921, 2557, 2617
BceAI ACGGC 5 cut(s) 101, 293, 523, 600, 942
BcgI CGANNNNNNTGC 4 cut(s) 468, 478, 502, 512
BciT130I CCWGG 4 cut(s) 79, 1539, 1710, 2106
BcnI CCSGG 3 cut(s) 167, 195, 808
BcoDI GTCTC 3 cut(s) 821, 836, 1974
BcuI ACTAGT 1 cut(s) 2183
BfaI CTAG 2 cut(s) 53, 2184
BfoI RGCGCY 4 cut(s) 130, 273, 378, 2155
BglII AGATCT 2 cut(s) 1434, 2317
BlpI GCTNAGC 1 cut(s) 2148
BmcAI AGTACT 3 cut(s) 227, 902, 2333
Bme1390I CCNGG 7 cut(s) 79, 167, 195, 808, 1539, 1710, 2106
Bme18I GGWCC 2 cut(s) 1167, 2079
BmeRI GACNNNNNGTC 1 cut(s) 244
BmeT110I CYCGRG 1 cut(s) 1922
BmgT120I GGNCC 5 cut(s) 151, 164, 197, 1167, 2079
BmiI GGNNCC 9 cut(s) 217, 271, 376, 654, 690, 718, 1089, 2397, 2619
BmrFI CCNGG 7 cut(s) 79, 167, 195, 808, 1539, 1710, 2106
BmrI ACTGGG 1 cut(s) 913
BmuI ACTGGG 1 cut(s) 913
BpiI GAAGAC 4 cut(s) 470, 1067, 2007, 2741
BplI GAGNNNNNCTC 4 cut(s) 116, 148, 626, 658
BpmI CTGGAG 2 cut(s) 30, 1765
Bpu10I CCTNAGC 2 cut(s) 2325, 2400
Bpu1102I GCTNAGC 1 cut(s) 2148
BpuEI CTTGAG 3 cut(s) 1430, 1551, 2356
BpuMI CCSGG 3 cut(s) 167, 195, 808
BsaHI GRCGYC 8 cut(s) 183, 240, 270, 363, 375, 485, 791, 1530
BsaI GGTCTC 2 cut(s) 821, 836
BsaJI CCNNGG 7 cut(s) 378, 834, 1071, 1525, 1722, 1923, 1987
BsaWI WCCGGW 4 cut(s) 218, 281, 368, 1090
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 6 cut(s) 908, 1637, 1782, 1836, 2496, 2769
Bse3DI GCAATG 1 cut(s) 2730
BseAI TCCGGA 2 cut(s) 281, 1090
BseBI CCWGG 4 cut(s) 79, 1539, 1710, 2106
BseDI CCNNGG 7 cut(s) 378, 834, 1071, 1525, 1722, 1923, 1987
BseGI GGATG 6 cut(s) 278, 318, 898, 964, 1503, 1641
BseMI GCAATG 1 cut(s) 2730
BseMII CTCAG 7 cut(s) 268, 1360, 1607, 2139, 2316, 2414, 2544
BseNI ACTGG 6 cut(s) 908, 1637, 1782, 1836, 2496, 2769
BseRI GAGGAG 4 cut(s) 1554, 1757, 1940, 2388
BseXI GCAGC 6 cut(s) 438, 456, 483, 1921, 2557, 2617
BseYI CCCAGC 2 cut(s) 153, 2613
Bsh1236I CGCG 2 cut(s) 335, 351
BshFI GGCC 7 cut(s) 116, 152, 165, 198, 359, 593, 1509
BshNI GGYRCC 3 cut(s) 269, 374, 652
BsiHKAI GWGCWC 2 cut(s) 1042, 1717
BsiHKCI CYCGRG 1 cut(s) 1922
BslFI GGGAC 3 cut(s) 449, 1517, 1643
BsmAI GTCTC 3 cut(s) 821, 836, 1974
BsmFI GGGAC 3 cut(s) 449, 1517, 1643
BsmI GAATGC 3 cut(s) 113, 1153, 1653
BsnI GGCC 7 cut(s) 116, 152, 165, 198, 359, 593, 1509
Bso31I GGTCTC 2 cut(s) 821, 836
BsoBI CYCGRG 1 cut(s) 1922
Bsp1286I GDGCHC 4 cut(s) 195, 1042, 1717, 2620
Bsp13I TCCGGA 2 cut(s) 281, 1090
Bsp1407I TGTACA 1 cut(s) 918
Bsp1720I GCTNAGC 1 cut(s) 2148
Bsp19I CCATGG 1 cut(s) 1722
BspANI GGCC 7 cut(s) 116, 152, 165, 198, 359, 593, 1509
BspCNI CTCAG 7 cut(s) 267, 1359, 1608, 2140, 2317, 2413, 2545
BspEI TCCGGA 2 cut(s) 281, 1090
BspFNI CGCG 2 cut(s) 335, 351
BspLI GGNNCC 9 cut(s) 217, 271, 376, 654, 690, 718, 1089, 2397, 2619
BspPI GGATC 9 cut(s) 296, 595, 683, 696, 1082, 1095, 1655, 1765, 2458
BspQI GCTCTTC 2 cut(s) 1722, 2252
BspT107I GGYRCC 3 cut(s) 269, 374, 652
BspTNI GGTCTC 2 cut(s) 821, 836
BsrBI CCGCTC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 2730
BsrFI RCCGGY 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 918
BsrI ACTGG 6 cut(s) 908, 1637, 1782, 1836, 2496, 2769
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 7 cut(s) 378, 834, 1071, 1525, 1722, 1923, 1987
BssNI GRCGYC 8 cut(s) 183, 240, 270, 363, 375, 485, 791, 1530
BssT1I CCWWGG 4 cut(s) 1071, 1525, 1722, 1987
Bst2UI CCWGG 4 cut(s) 79, 1539, 1710, 2106
Bst4CI ACNGT 5 cut(s) 652, 838, 1229, 2412, 2429
Bst6I CTCTTC 3 cut(s) 649, 1722, 2252
BstACI GRCGYC 8 cut(s) 183, 240, 270, 363, 375, 485, 791, 1530
BstAUI TGTACA 1 cut(s) 918
BstDSI CCRYGG 2 cut(s) 834, 1722
BstENI CCTNNNNNAGG 2 cut(s) 32, 1875
BstF5I GGATG 6 cut(s) 278, 318, 898, 964, 1503, 1641
BstFNI CGCG 2 cut(s) 335, 351
BstH2I RGCGCY 4 cut(s) 130, 273, 378, 2155
BstMAI GTCTC 3 cut(s) 821, 836, 1974
BstNI CCWGG 4 cut(s) 79, 1539, 1710, 2106
BstNSI RCATGY 1 cut(s) 2027
BstSCI CCNGG 7 cut(s) 77, 165, 193, 806, 1537, 1708, 2104
BstUI CGCG 2 cut(s) 335, 351
BstV1I GCAGC 6 cut(s) 438, 456, 483, 1921, 2557, 2617
BstV2I GAAGAC 4 cut(s) 470, 1067, 2007, 2741
BstX2I RGATCY 5 cut(s) 688, 1087, 1434, 1660, 2317
BstXI CCANNNNNNTGG 2 cut(s) 673, 838
BstYI RGATCY 5 cut(s) 688, 1087, 1434, 1660, 2317
BsuRI GGCC 7 cut(s) 116, 152, 165, 198, 359, 593, 1509
BtgI CCRYGG 2 cut(s) 834, 1722
BtgZI GCGATG 2 cut(s) 316, 1482
BtsCI GGATG 6 cut(s) 278, 318, 898, 964, 1503, 1641
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 6 cut(s) 486, 1234, 1789, 2503, 2644, 2762
CaiI CAGNNNCTG 1 cut(s) 610
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 5 cut(s) 151, 164, 197, 1167, 2079
CseI GACGC 7 cut(s) 191, 248, 371, 475, 799, 1221, 1519
Csp6I GTAC 4 cut(s) 226, 901, 919, 2332
CspCI CAANNNNNGTGG 4 cut(s) 1890, 1925, 2562, 2597
CviAII CATG 9 cut(s) 338, 721, 812, 1594, 1723, 2024, 2599, 2711, 2731
CviQI GTAC 4 cut(s) 226, 901, 919, 2332
DinI GGCGCC 2 cut(s) 271, 376
DrdI GACNNNNNNGTC 1 cut(s) 472
DriI GACNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 472
Eam1104I CTCTTC 3 cut(s) 649, 1722, 2252
Eam1105I GACNNNNNGTC 1 cut(s) 244
EarI CTCTTC 3 cut(s) 649, 1722, 2252
EciI GGCGGA 5 cut(s) 653, 692, 872, 971, 1100
Ecl136II GAGCTC 2 cut(s) 1040, 1715
Eco130I CCWWGG 4 cut(s) 1071, 1525, 1722, 1987
Eco147I AGGCCT 1 cut(s) 593
Eco24I GRGCYC 4 cut(s) 195, 1042, 1717, 2620
Eco31I GGTCTC 2 cut(s) 821, 836
Eco32I GATATC 2 cut(s) 547, 2799
Eco47I GGWCC 2 cut(s) 1167, 2079
Eco47III AGCGCT 1 cut(s) 2153
Eco53kI GAGCTC 2 cut(s) 1040, 1715
Eco57I CTGAAG 7 cut(s) 311, 482, 506, 1704, 1794, 1823, 2620
Eco88I CYCGRG 1 cut(s) 1922
EcoICRI GAGCTC 2 cut(s) 1040, 1715
EcoNI CCTNNNNNAGG 2 cut(s) 32, 1875
EcoO109I RGGNCCY 1 cut(s) 2079
EcoRII CCWGG 4 cut(s) 77, 1537, 1708, 2104
EcoRV GATATC 2 cut(s) 547, 2799
EcoT14I CCWWGG 4 cut(s) 1071, 1525, 1722, 1987
EcoT22I ATGCAT 2 cut(s) 1954, 2025
EcoT38I GRGCYC 4 cut(s) 195, 1042, 1717, 2620
EgeI GGCGCC 2 cut(s) 271, 376
EheI GGCGCC 2 cut(s) 271, 376
ErhI CCWWGG 4 cut(s) 1071, 1525, 1722, 1987
FaeI CATG 9 cut(s) 341, 724, 815, 1597, 1726, 2027, 2602, 2714, 2734
FalI AAGNNNNNCTT 2 cut(s) 1187, 1219
FaqI GGGAC 3 cut(s) 449, 1517, 1643
FatI CATG 9 cut(s) 337, 720, 811, 1593, 1722, 2023, 2598, 2710, 2730
FauI CCCGC 5 cut(s) 32, 166, 207, 313, 1074
FauNDI CATATG 1 cut(s) 2517
FblI GTMKAC 1 cut(s) 1443
FokI GGATG 6 cut(s) 265, 305, 905, 971, 1510, 1648
FriOI GRGCYC 4 cut(s) 195, 1042, 1717, 2620
FspAI RTGCGCAY 1 cut(s) 2241
FspBI CTAG 2 cut(s) 53, 2184
FspI TGCGCA 3 cut(s) 1013, 1652, 2241
GsaI CCCAGC 2 cut(s) 157, 2617
GsuI CTGGAG 2 cut(s) 30, 1765
HaeII RGCGCY 4 cut(s) 130, 273, 378, 2155
HaeIII GGCC 7 cut(s) 116, 152, 165, 198, 359, 593, 1509
HgaI GACGC 7 cut(s) 191, 248, 371, 475, 799, 1221, 1519
Hin1I GRCGYC 8 cut(s) 183, 240, 270, 363, 375, 485, 791, 1530
Hin1II CATG 9 cut(s) 341, 724, 815, 1597, 1726, 2027, 2602, 2714, 2734
HincII GTYRAC 2 cut(s) 1236, 1444
HindII GTYRAC 2 cut(s) 1236, 1444
HindIII AAGCTT 1 cut(s) 800
HphI GGTGA 7 cut(s) 233, 625, 1751, 1911, 2120, 2266, 2428
Hpy166II GTNNAC 6 cut(s) 1236, 1444, 2419, 2471, 2563, 2775
Hpy8I GTNNAC 6 cut(s) 1236, 1444, 2419, 2471, 2563, 2775
Hpy99I CGWCG 9 cut(s) 185, 212, 242, 314, 365, 490, 586, 790, 793
HpyAV CCTTC 7 cut(s) 583, 1096, 1520, 1748, 1838, 2644, 2684
HpyCH4III ACNGT 5 cut(s) 652, 838, 1229, 2412, 2429
HpyCH4IV ACGT 2 cut(s) 485, 1553
HpySE526I ACGT 2 cut(s) 485, 1553
Hsp92I GRCGYC 8 cut(s) 183, 240, 270, 363, 375, 485, 791, 1530
Hsp92II CATG 9 cut(s) 341, 724, 815, 1597, 1726, 2027, 2602, 2714, 2734
KasI GGCGCC 2 cut(s) 269, 374
Kpn2I TCCGGA 2 cut(s) 281, 1090
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
LguI GCTCTTC 2 cut(s) 1722, 2252
LmnI GCTCC 8 cut(s) 207, 221, 448, 722, 1123, 1712, 2401, 2623
Lsp1109I GCAGC 6 cut(s) 438, 456, 483, 1921, 2557, 2617
MaeI CTAG 2 cut(s) 53, 2184
MaeII ACGT 2 cut(s) 485, 1553
MaeIII GTNAC 3 cut(s) 481, 2713, 2755
MbiI CCGCTC 1 cut(s) 202
MfeI CAATTG 2 cut(s) 1646, 2486
MflI RGATCY 5 cut(s) 688, 1087, 1434, 1660, 2317
MhlI GDGCHC 4 cut(s) 195, 1042, 1717, 2620
Mly113I GGCGCC 2 cut(s) 270, 375
MlyI GAGTC 6 cut(s) 254, 416, 635, 863, 1754, 2564
MmeI TCCRAC 5 cut(s) 55, 159, 809, 870, 1122
Mph1103I ATGCAT 2 cut(s) 1954, 2025
MroI TCCGGA 2 cut(s) 281, 1090
MroNI GCCGGC 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 2694
MseI TTAA 5 cut(s) 660, 1194, 1220, 1473, 1515
MslI CAYNNNNRTG 5 cut(s) 671, 725, 836, 2028, 2745
MspA1I CMGCKG 1 cut(s) 2273
MspR9I CCNGG 7 cut(s) 79, 167, 195, 808, 1539, 1710, 2106
MunI CAATTG 2 cut(s) 1646, 2486
Mva1269I GAATGC 3 cut(s) 113, 1153, 1653
MvaI CCWGG 4 cut(s) 79, 1539, 1710, 2106
MvnI CGCG 2 cut(s) 335, 351
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 2 cut(s) 270, 375
NciI CCSGG 3 cut(s) 167, 195, 808
NcoI CCATGG 1 cut(s) 1722
NdeI CATATG 1 cut(s) 2517
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 9 cut(s) 341, 724, 815, 1597, 1726, 2027, 2602, 2714, 2734
NlaIV GGNNCC 9 cut(s) 217, 271, 376, 654, 690, 718, 1089, 2397, 2619
NmeAIII GCCGAG 3 cut(s) 268, 403, 1844
NmuCI GTSAC 2 cut(s) 481, 2755
NsbI TGCGCA 3 cut(s) 1013, 1652, 2241
NsiI ATGCAT 2 cut(s) 1954, 2025
NspI RCATGY 1 cut(s) 2027
OliI CACNNNNGTG 1 cut(s) 836
PaeI GCATGC 1 cut(s) 2027
PceI AGGCCT 1 cut(s) 593
PciSI GCTCTTC 2 cut(s) 1722, 2252
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 3 cut(s) 113, 1153, 1653
PdiI GCCGGC 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 2694
PflMI CCANNNNNTGG 4 cut(s) 430, 610, 903, 1438
PleI GAGTC 6 cut(s) 253, 415, 635, 863, 1753, 2563
PluTI GGCGCC 2 cut(s) 273, 378
PpsI GAGTC 6 cut(s) 253, 415, 635, 863, 1753, 2563
PpuMI RGGWCCY 1 cut(s) 2079
PshBI ATTAAT 1 cut(s) 660
PsiI TTATAA 1 cut(s) 2285
Psp124BI GAGCTC 2 cut(s) 1042, 1717
Psp5II RGGWCCY 1 cut(s) 2079
Psp6I CCWGG 4 cut(s) 77, 1537, 1708, 2104
PspFI CCCAGC 2 cut(s) 153, 2613
PspGI CCWGG 4 cut(s) 77, 1537, 1708, 2104
PspN4I GGNNCC 9 cut(s) 217, 271, 376, 654, 690, 718, 1089, 2397, 2619
PspPI GGNCC 5 cut(s) 151, 164, 197, 1167, 2079
PspPPI RGGWCCY 1 cut(s) 2079
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 5 cut(s) 688, 1087, 1434, 1660, 2317
PvuII CAGCTG 1 cut(s) 2273
RsaI GTAC 4 cut(s) 227, 902, 920, 2333
RsaNI GTAC 4 cut(s) 226, 901, 919, 2332
RseI CAYNNNNRTG 5 cut(s) 671, 725, 836, 2028, 2745
SacI GAGCTC 2 cut(s) 1042, 1717
SalI GTCGAC 1 cut(s) 1442
SapI GCTCTTC 2 cut(s) 1722, 2252
SaqAI TTAA 5 cut(s) 660, 1194, 1220, 1473, 1515
Sau96I GGNCC 5 cut(s) 151, 164, 197, 1167, 2079
ScaI AGTACT 3 cut(s) 227, 902, 2333
SchI GAGTC 6 cut(s) 254, 416, 635, 863, 1754, 2564
ScrFI CCNGG 7 cut(s) 79, 167, 195, 808, 1539, 1710, 2106
SduI GDGCHC 4 cut(s) 195, 1042, 1717, 2620
SfoI GGCGCC 2 cut(s) 271, 376
SinI GGWCC 2 cut(s) 1167, 2079
SmiMI CAYNNNNRTG 5 cut(s) 671, 725, 836, 2028, 2745
SmlI CTYRAG 3 cut(s) 1409, 1566, 2335
SmoI CTYRAG 3 cut(s) 1409, 1566, 2335
SpeI ACTAGT 1 cut(s) 2183
SphI GCATGC 1 cut(s) 2027
SseBI AGGCCT 1 cut(s) 593
SspDI GGCGCC 2 cut(s) 269, 374
SspI AATATT 1 cut(s) 2458
SspMI CTAG 2 cut(s) 53, 2184
SstI GAGCTC 2 cut(s) 1042, 1717
StuI AGGCCT 1 cut(s) 593
StyD4I CCNGG 7 cut(s) 77, 165, 193, 806, 1537, 1708, 2104
StyI CCWWGG 4 cut(s) 1071, 1525, 1722, 1987
TaaI ACNGT 5 cut(s) 652, 838, 1229, 2412, 2429
TaiI ACGT 2 cut(s) 488, 1556
TaqI TCGA 9 cut(s) 123, 248, 312, 405, 488, 825, 872, 1443, 1809
TatI WGTACW 4 cut(s) 225, 900, 918, 2331
TauI GCSGC 5 cut(s) 152, 275, 278, 354, 2723
Tru1I TTAA 5 cut(s) 660, 1194, 1220, 1473, 1515
Tru9I TTAA 5 cut(s) 660, 1194, 1220, 1473, 1515
TscAI CASTG 6 cut(s) 486, 1234, 1789, 2503, 2644, 2769
TseFI GTSAC 2 cut(s) 481, 2755
TseI GCWGC 6 cut(s) 451, 469, 496, 1934, 2570, 2630
Tsp45I GTSAC 2 cut(s) 481, 2755
TspGWI ACGGA 4 cut(s) 225, 400, 2206, 2456
TspRI CASTG 6 cut(s) 486, 1234, 1789, 2503, 2644, 2769
Van91I CCANNNNNTGG 4 cut(s) 430, 610, 903, 1438
VpaK11BI GGWCC 2 cut(s) 1167, 2079
VspI ATTAAT 1 cut(s) 660
XagI CCTNNNNNAGG 2 cut(s) 32, 1875
XceI RCATGY 1 cut(s) 2027
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XmiI GTMKAC 1 cut(s) 1443
XmnI GAANNNNTTC 1 cut(s) 2694
XspI CTAG 2 cut(s) 53, 2184
ZraI GACGTC 1 cut(s) 486
ZrmI AGTACT 3 cut(s) 227, 902, 2333
Zsp2I ATGCAT 2 cut(s) 1954, 2025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.