AT3G60860

Guanine nucleotide exchange family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007735
Physical Location & Seq
Forward (+)
22484397 .. 22491935
7539 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60860.1

Sequence Viewer

Length: 5382 bp
ATGGCTTCCTCGGAAGCTGATTCCCGATTGAGTCGAGTCGTAACACCAGCTTTAGAGAAGATCGTCAAGAACGCGTCGTGGCGTAAACACTCGAAGCTAGCTAACGAATGCAAAGCTGTTATCGAGCGGCTAAATTCGCTGCAGAAATCACCACCTCCTTCGTCCTCTGCTGCTACTGACTCCGAGTCTGAATCCTCTGTTCCCGGTCCACTTAACGACGGTGGTTCGATCGAGTACTCGCTCGCCGATTCGGAACTCATCTTCAGCCCTCTGATCAATGCCTGTGGCACTGGCCTCGCCAAGATCATCGAACCTGCTATTGACTGCATTCAGAAACTTATCGCGCATGGTTACATTCGTGGTGAATCGGACCCGTCCGGTGGAGCGGAGTCCCTTCTCCTTTTTAAATTGATCGATTCCGTTTGTAAATGCCATGATTTGGGAGATGAATCCATTGAATTACCTGTGTTAAAGACTCTGTTATCTGCCATTAACTCCATCTCGCTGAGGATCCATGGCAAATGCTTGCTCCTAGTTGTTCGTACTTGCTACGACATTTACTTGGGAAGCAAAAACGTGGTGAATCAGACGACTGCCAAGGCTTCGCTGATCCAGATTCTGGTGATTGTTTTCCGGAGAATGGAGGCGGATTCGTCAACGGTTCCAATTCAGCCGATTGTAGTGGCTGAGCTCATGGAGCCACTCGAAAAATCAGATGCGGATGGGACAATGACGCAGTTTGTTCAGGGTTTCATCACCAAAATCATGCAAGATATTGACGGAGTGTTGAACCCGACAATGTCAGGATCAGGATCAGGATCAGGGTCAGGAGGCCAAGATGGGGCATATGGGACTACCACAGTGGAGACTACGAATCCTACGGATTTGTTGGACTCCACTGATAAGGACATGTTGGACGCTAAGTACTGGGAGATCAGTATGTACAAGTCAGCTCTAGAGGGAAGGAAGGGGGAGCTAACTGACGGAGACGCTGAGAGAGATGATGATTTAGAAGTTCAGATAGAGAATAAGCTGCGGAGAGATGCCTGCCTGGTTTTCAGGGCGCTTTGTAAGCTCTCCATGAAGGCGCCGCCAAAGGAGTCTTCGGCAGATCCGCAGTCGATGAGAGGGAAGATTCTGGCTCTTGAGCTGTTAAAGATCTTGTTGGAAAATGCTGGAGCTGTCTTTAGAACAAGCGAGAAGTTTTCAGCTGATATCAAACAGTTTTTGTGTTTGTCGTTGTTGAAGAACAGTGCTTCTACTCTTATGATTATCTTCCAGCTTTCTTGCTCTATTTTCATCAGTCTGGTTGCAAGATTCCGGGCTGGATTAAAGGCCGAAATTGGAGTGTTTTTTCCAATGATTGTTCTTAGAGTCGTGGAGAATGTAGCTCAACCTAATTTTCAACAAAAGATGATTGTTCTTCGGTTTCTTGACAAACTTTGTTTGGATTCACAAATCCTAGTTGATATATTCTTGAACTACGATTGCGATGTCAATTCATCAAATATCTTTGAGAGGATGGTCAATGGTCTCCTTAAAACGGCTCAAGGGGTTCCTCCTGGTACAGCTACAACATTGATGCCACCACAGGAGGCAGCTATGAAACTTGAAGCCATGAAATGCTTAGTGGCCATATTGAAATCAATGGGAGATTGGTTGAACAAGCAGTTGCGCCTGCCAGTTTCTAATTCATTGAATAAATCTGACGTAATTGAGATTGATTTGGGACCCGGAAGTCCCCAGTTGGCAAATGGAAATGCTGATGAGTCTGCTGACGGATCAGATACTTATTCTGAATCTTCAGGAGGTACATCTGATGCTCTAGCTATCGAGCAGCGCCGGGCTTACAAACTCGAGCTTCAGGAAGGTATTTCTCTTTTTAATCGGAAGCCAACTAAAGGTATTGAGTTTCTCATCAATGCGGGTAAGGTGGGTGAATCTCCTGAAGAAATAGCAGGTTTTCTCAAAGACGCGTCAGGGTTAAATAAAACTTTGATAGGCGACTATCTTGGAGAAAGAGAAGATTTAGCTCTCAAGGTGATGCATGCATATGTGGATTCTTTTGATTTCCGAGGCATGGAATTTGATGAGGCAATTAGAACCTTTCTTGAAGGTTTTAGGCTGCCAGGAGAAGCACAAAAGATTGACCGAATCATGGAAAAGTTTGCTGAACGTTACTGCAAGTGTAATCCTAAGGTGTTTACTAGCGCAGATTCAGCTTATGTTCTTGCATATTCTGTGATCATGCTCAATACTGATGCCCACAACCCCATGGTGAAGAACAAGATGTCTGCTGATGACTTTATCAGAAACAATCGTGGAATAGACGACGGAAAAGATTTACCTGCGGACTACATGAGATCGCTGTATGAAAGGATAACAAAGCATGAGATAAAGATGAAAGAAGATGATTTACGTTTGCAACAGAAACAGTACGCAAACTCAAACAGAATGCTTGGTTTGGATGGTATATTAAACATAGTGATCCGTAAGCAGTGGGGAGATTCATATGCGGAAACAAGTGATGATCTGATGAAGCATATGCAAGAGCAATTTAAGGAAAAAGCTCGTAAATCAGAGTCAACCTACTATGCTGCTACAGATGTGGTTATTCTCAGATTTATGATTGAGGCTTGTTGGGCTCCTATGCTTGCTGCATTCAGTGTGCCGCTAGACCAGAGTGATGATTTGATAGTGATCAACATATGTCTTGAAGGCTTCCACCATGCAATCCATGCCACTTCGTTGATGTCAATGAAGACCCATAGAGATGCTTTTGTGACTTCACTAGCGAAGTTCACTTCACTTCACTCCCCAGCTGATATCAAGCAGAGAAATATTGAAGCCATTAAGGCGATTCTCAGACTTGCTGATGAAGAGGGCAACTATTTGCAAGATGCCTGGGAGCATATACTAACTTGTGTTTCCCGTTTTGAGCAACTACATCTCTTGGGAGAAGGTGCTCCTCCAGATGCCACTTTTTTTGCTTCCAAGCAAAATGAGTCAGAGAAATCAAAGCAACCCAAACAGTATATACTTCCTGTTCTGAAAAGGAAGGGTCCTGGGAAAAGTCAATATGCAGCTACAGGTGTCTTGAGGGGTTCATATGACAGCATGAGTCTTGGCGGAAAAGGTTCTAAGAACGTGAGACAAGAACAGATGAGCAGTATAGTCTCAAATTTGAACTTGTTGGAGCAGGTTGGTGAAATGAATCAAGTATTTTCTCAGAGTCAGAAATTAAACAGTGAAGCTATAATAGACTTTGTTAAAGCTCTCTGCAAAGTTTCTATGGATGAGTTGCGGTCTCCTTCTAATCCACGCGTTTTTAGCCTCACAAAGATTGTTGAGATAGCGCATTATAACATGAATCGCATAAGGCTTGTTTGGTCAAGCATCTGGCAAGTGCTATCAGGTTTCTTTGTGACCATTGGATGCTCAGAGAATCTTTCTATTGCAATATTTGCAATGGATTCTCTCCGCCAGTTGTCAATGAAATTTCTGGAACGGGAGGAGTTGGCTAATTACAACTTCCAAAATGAATTTATGACACCTTTTGTCATTGTTATGCGTAGAAGTAATGATGTTGAGATCAGAGAACTTATCATCCGATGTGTTTCTCAGATGGTATTGTCACGTGTAAATAATGTTAAGTCAGGATGGAAAAGCATGTTTATGGTTTTTACCACAGCGGCATATGACGACCACAAAAATATTGTGTTTTTGTCCTTTGAAATTATCGAGAAGATTATTCGAGAATATTTCCCATACATCACTGAGACAGAAACCACCACTTTTACAGATTGTGTGAATTGCCTAGTTGCATTCACCAATAACAGATTTAGCAAGGATATCAGTCTCAGTTCTATAGCTTTCCTCCGGTATTGTGCAACAAAGCTTGCAGAAGGAGATCTTAACTCGCCATCAACAAACAAATACAAGGGAACTTCTGGAAAGATTCCTCAGTCATCTCTCCACAGTGGAAAAAGTGGGAAACAAGAGAACGGAGAGATTGTAAACAACAATCATCTTTATTTTTGGTTTCCCTTACTATCAGGTTTATCAGAGCTTAGTTTCGATCCAAGACCAGAAATCAGGAAAAGCGCACTACAGATTATGTTTGATACCTTACGCAACCATGGCCATTTATTTTCTCTGCCGTTATGGGAAAAAGTATTCGAGTCTGTATTATTTCCTATATTCGACTACGTACGCCATTCTATTGATCCTTCTGGCGAAGACGAATCTGCTGATCAAGGAAGTTCTGGTGGTGAAGTGGATGAGCTAGATCATGACGCCTGGCTCTACGAGACCTGTACATTAGCACTACAGCTTGTAGTGGATCTCTTTGTCAAATTTTACACTACAGTCAATCCACTTCTAGAGAAAGTACTAATGCTTCTTGTAAGCTTCATAAAGAGACCTCACCAGAGCCTTGCAGGAATTGGTATTGCTGCGTTTGTTCGTCTGATGAGCGATGCTGATGGCTTGTTCTCTGAAGAAAAGTGGTTAGAGGTGGTGTCAGCTTTAAAAGAAGCGGCGAAAACAACTTGCCCAGATTTTTCGTATTTCCTTAGCGAAGAATATGTAGCAAGAAGCCAGAGAAGTGCTCTCAACATTCAGAACAGTAATGCTGAGTCTGCTGCTCCAACTGCTACCGATGGCAATGAAGAGAGTCAGAGAACAGCAACCCATCTTTACGCTGCTATCTCTGATGCTAAGTGCCGAGCCGCTGTTCAACTTTTGTTGATCCAGGCTGTGATGGAGATTTACAACATGTACAGACCTCAGCTTTCAGCGAAAAACACATTAGTCCTCGTAGATGCATTGCACGGTGTAGCTTTACATGCTCACGGAATAAACAGTAACACAATACTTCGTTCAAGACTACAAGAGCTAGGACCCATGACACAGATGCAAGATCCTCCACTACTTCGTCTTGAAAACGAGTCTTACCAAATCTGCCTGACATTCTTACAGAATCTTGTTGCAGACAAAACCAAAAAAGAAGAAGAAGAAGAAGAAGAGGAAATAGAATCACTTCTAGTCAACATCTGTCAAGAAGTTCTCAACTTCTACATCGAGACTTCTTCTTCTGCGAAAAAATTACAATCAGAATCATCACGGGCCAGCGAATACCGATGGAGAATACCTTTGGGATCAGGAAAACGAAGAGAACTATCAGCAAGAGCTCCTCTTATAGTTGCAACACTTCAAGCAATGTGTACATTGGATGAGGCATCATTTGAGAAGAACCTGAAATGTCTATTTCCACTTCTGGCTAACTTGATTAGCTGCGAACACGGCTCTAACGAGGTTCAGACTGCTCTTGCTGACATGCTTGGCCTATCGGTTGGCCCGGTTTTACTCCAATGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1793

Amino Acids

199.63

Weight (kDa)

5.37

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 6 - 217 7.1e-38 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 346 - 504 2.1e-45 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 618 - 799 5.8e-78 Sec7 domain
Sec7-like_HDS PF09324 1166 - 1248 1.5e-29 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1345 - 1421 1.4e-11 C-terminal region of Mon2 protein
BIG2_C PF20252 1578 - 1781 2.7e-42 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3363
AasI GACNNNNNNGTC 1 cut(s) 1737
Acc36I ACCTGC 4 cut(s) 322, 1949, 2356, 3190
AccB1I GGYRCC 1 cut(s) 1087
AccB7I CCANNNNNTGG 2 cut(s) 439, 619
AccBSI CCGCTC 2 cut(s) 127, 386
AccII CGCG 4 cut(s) 74, 344, 1976, 3324
AccIII TCCGGA 1 cut(s) 633
AclI AACGTT 1 cut(s) 2176
AcoI YGGCCR 2 cut(s) 1632, 4141
AcsI RAATTY 6 cut(s) 133, 2084, 3181, 3497, 3542, 4355
AcuI CTGAAG 5 cut(s) 247, 1788, 1847, 1968, 4518
AcvI CACGTG 1 cut(s) 3638
AcyI GRCGYC 2 cut(s) 1088, 4296
AfiI CCNNNNNNNGG 9 cut(s) 380, 439, 619, 640, 841, 1543, 1901, 2080, 2158
AflIII ACRYGT 6 cut(s) 72, 909, 1974, 3322, 3637, 4776
AhdI GACNNNNNGTC 1 cut(s) 184
AjnI CCWGG 7 cut(s) 1050, 1561, 2128, 2903, 3064, 4298, 4752
AjuI GAANNNNNNNTTGG 2 cut(s) 1087, 1119
AloI GAACNNNNNNTCC 2 cut(s) 3591, 3623
Alw21I GWGCWC 4 cut(s) 693, 2968, 4612, 5194
AlwNI CAGNNNCTG 1 cut(s) 619
Ama87I CYCGRG 1 cut(s) 1856
Aor13HI TCCGGA 1 cut(s) 633
AoxI GGCC 8 cut(s) 292, 832, 1335, 1632, 4141, 5127, 5344, 5356
ApoI RAATTY 6 cut(s) 133, 2084, 3181, 3497, 3542, 4355
Asp700I GAANNNNTTC 2 cut(s) 3136, 5040
AspLEI GCGC 8 cut(s) 346, 1066, 1090, 1677, 1842, 2213, 3358, 4106
AspS9I GGNCC 7 cut(s) 206, 370, 1730, 3062, 4901, 5127, 5357
AsuC2I CCSGG 5 cut(s) 204, 1322, 1734, 1844, 5360
AsuNHI GCTAGC 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 1856
AvaII GGWCC 5 cut(s) 206, 370, 1730, 3062, 4901
AxyI CCTNAGG 1 cut(s) 2196
BalI TGGCCA 2 cut(s) 1634, 4143
BamHI GGATCC 1 cut(s) 510
BanI GGYRCC 1 cut(s) 1087
BanII GRGCYC 3 cut(s) 693, 2647, 5194
BarI GAAGNNNNNNTAC 4 cut(s) 2400, 2432, 4383, 4415
BbrPI CACGTG 1 cut(s) 3638
BbsI GAAGAC 3 cut(s) 1095, 2768, 4245
Bbv12I GWGCWC 4 cut(s) 693, 2968, 4612, 5194
BbvCI CCTCAGC 2 cut(s) 506, 4788
BceAI ACGGC 3 cut(s) 1560, 4144, 5320
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 7 cut(s) 1052, 1563, 2130, 2905, 3066, 4300, 4754
BclI TGATCA 4 cut(s) 273, 2244, 2700, 4252
BcnI CCSGG 5 cut(s) 204, 1322, 1734, 1844, 5360
BfmI CTRYAG 7 cut(s) 140, 2601, 3087, 3867, 4109, 4328, 4365
BfoI RGCGCY 3 cut(s) 1067, 1091, 1843
BfuAI ACCTGC 4 cut(s) 322, 1949, 2356, 3190
BglI GCCNNNNNGGC 1 cut(s) 2855
BglII AGATCT 2 cut(s) 1158, 3910
BlpI GCTNAGC 1 cut(s) 687
BmcAI AGTACT 3 cut(s) 236, 926, 4392
Bme18I GGWCC 5 cut(s) 206, 370, 1730, 3062, 4901
BmeRI GACNNNNNGTC 1 cut(s) 184
BmeT110I CYCGRG 1 cut(s) 1856
BmgT120I GGNCC 7 cut(s) 206, 370, 1730, 3062, 4901, 5127, 5357
BmrI ACTGGG 2 cut(s) 937, 1738
BmtI GCTAGC 1 cut(s) 101
BmuI ACTGGG 2 cut(s) 937, 1738
BpiI GAAGAC 3 cut(s) 1095, 2768, 4245
BplI GAGNNNNNCTC 2 cut(s) 4594, 4626
BpmI CTGGAG 2 cut(s) 1197, 2955
Bpu10I CCTNAGC 3 cut(s) 506, 4574, 4788
Bpu1102I GCTNAGC 1 cut(s) 687
BpuEI CTTGAG 4 cut(s) 1166, 1533, 2021, 3118
BpuMI CCSGG 5 cut(s) 204, 1322, 1734, 1844, 5360
Bsa29I ATCGAT 1 cut(s) 414
BsaAI YACGTR 2 cut(s) 3638, 4210
BsaBI GATNNNNATC 3 cut(s) 811, 817, 2699
BsaHI GRCGYC 2 cut(s) 1088, 4296
BsaI GGTCTC 4 cut(s) 1538, 3312, 4304, 4414
BsaJI CCNNGG 8 cut(s) 9, 514, 597, 2074, 2274, 2904, 3065, 4138
BsaWI WCCGGW 3 cut(s) 377, 633, 3879
BsaXI ACNNNNNCTCC 2 cut(s) 3999, 4029
Bsc4I CCNNNNNNNGG 9 cut(s) 380, 439, 619, 640, 841, 1543, 1901, 2080, 2158
Bse1I ACTGG 5 cut(s) 295, 932, 1682, 1744, 3484
Bse21I CCTNAGG 1 cut(s) 2196
Bse3DI GCAATG 4 cut(s) 3474, 4672, 4826, 5226
Bse8I GATNNNNATC 3 cut(s) 811, 817, 2699
BseAI TCCGGA 1 cut(s) 633
BseBI CCWGG 7 cut(s) 1052, 1563, 2130, 2905, 3066, 4300, 4754
BseCI ATCGAT 1 cut(s) 414
BseDI CCNNGG 8 cut(s) 9, 514, 597, 2074, 2274, 2904, 3065, 4138
BseGI GGATG 9 cut(s) 727, 1527, 2473, 3301, 3440, 3606, 3665, 4285, 5239
BseJI GATNNNNATC 3 cut(s) 811, 817, 2699
BseLI CCNNNNNNNGG 9 cut(s) 380, 439, 619, 640, 841, 1543, 1901, 2080, 2158
BseMI GCAATG 4 cut(s) 3474, 4672, 4826, 5226
BseNI ACTGG 5 cut(s) 295, 932, 1682, 1744, 3484
BseRI GAGGAG 3 cut(s) 2958, 3527, 5184
BseYI CCCAGC 1 cut(s) 2818
Bsh1236I CGCG 4 cut(s) 74, 344, 1976, 3324
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 8 cut(s) 294, 834, 1337, 1634, 4143, 5129, 5346, 5358
BshNI GGYRCC 1 cut(s) 1087
BshVI ATCGAT 1 cut(s) 414
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 4 cut(s) 693, 2968, 4612, 5194
BsiHKCI CYCGRG 1 cut(s) 1856
BsiSI CCGG 8 cut(s) 204, 378, 634, 1321, 1734, 1843, 3880, 5360
BsiWI CGTACG 1 cut(s) 4210
BslFI GGGAC 5 cut(s) 376, 739, 865, 1725, 1743
BslI CCNNNNNNNGG 9 cut(s) 380, 439, 619, 640, 841, 1543, 1901, 2080, 2158
BsmBI CGTCTC 1 cut(s) 981
BsmFI GGGAC 5 cut(s) 376, 739, 865, 1725, 1743
BsmI GAATGC 5 cut(s) 113, 327, 2460, 2660, 3824
BsnI GGCC 8 cut(s) 294, 834, 1337, 1634, 4143, 5129, 5346, 5358
Bso31I GGTCTC 4 cut(s) 1538, 3312, 4304, 4414
BsoBI CYCGRG 1 cut(s) 1856
Bsp1286I GDGCHC 5 cut(s) 693, 2647, 2968, 4612, 5194
Bsp13I TCCGGA 1 cut(s) 633
Bsp1407I TGTACA 4 cut(s) 942, 4316, 4779, 5225
Bsp1720I GCTNAGC 1 cut(s) 687
Bsp19I CCATGG 3 cut(s) 514, 2274, 4138
BspANI GGCC 8 cut(s) 294, 834, 1337, 1634, 4143, 5129, 5346, 5358
BspDI ATCGAT 1 cut(s) 414
BspEI TCCGGA 1 cut(s) 633
BspFNI CGCG 4 cut(s) 74, 344, 1976, 3324
BspHI TCATGA 1 cut(s) 4291
BspMAI CTGCAG 1 cut(s) 144
BspMI ACCTGC 4 cut(s) 322, 1949, 2356, 3190
BspOI GCTAGC 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 1087
BspTNI GGTCTC 4 cut(s) 1538, 3312, 4304, 4414
BsrBI CCGCTC 2 cut(s) 127, 386
BsrDI GCAATG 4 cut(s) 3474, 4672, 4826, 5226
BsrGI TGTACA 4 cut(s) 942, 4316, 4779, 5225
BsrI ACTGG 5 cut(s) 295, 932, 1682, 1744, 3484
BssECI CCNNGG 8 cut(s) 9, 514, 597, 2074, 2274, 2904, 3065, 4138
BssNI GRCGYC 2 cut(s) 1088, 4296
BssT1I CCWWGG 4 cut(s) 514, 597, 2274, 4138
Bst2UI CCWGG 7 cut(s) 1052, 1563, 2130, 2905, 3066, 4300, 4754
Bst6I CTCTTC 4 cut(s) 2874, 4665, 5019, 5167
BstACI GRCGYC 2 cut(s) 1088, 4296
BstAPI GCANNNNNTGC 2 cut(s) 2738, 3464
BstAUI TGTACA 4 cut(s) 942, 4316, 4779, 5225
BstBAI YACGTR 2 cut(s) 3638, 4210
BstC8I GCNNGC 9 cut(s) 99, 243, 527, 1048, 1679, 2049, 2655, 3900, 5131
BstDSI CCRYGG 3 cut(s) 514, 2274, 4138
BstF5I GGATG 9 cut(s) 727, 1527, 2473, 3301, 3440, 3606, 3665, 4285, 5239
BstFNI CGCG 4 cut(s) 74, 344, 1976, 3324
BstH2I RGCGCY 3 cut(s) 1067, 1091, 1843
BstHHI GCGC 8 cut(s) 346, 1066, 1090, 1677, 1842, 2213, 3358, 4106
BstMCI CGRYCG 1 cut(s) 231
BstNI CCWGG 7 cut(s) 1052, 1563, 2130, 2905, 3066, 4300, 4754
BstNSI RCATGY 6 cut(s) 913, 2051, 3673, 4780, 4850, 5341
BstSFI CTRYAG 7 cut(s) 140, 2601, 3087, 3867, 4109, 4328, 4365
BstSNI TACGTA 1 cut(s) 4210
BstUI CGCG 4 cut(s) 74, 344, 1976, 3324
BstV2I GAAGAC 3 cut(s) 1095, 2768, 4245
BstX2I RGATCY 6 cut(s) 510, 1111, 1158, 3910, 4342, 4921
BstYI RGATCY 6 cut(s) 510, 1111, 1158, 3910, 4342, 4921
Bsu15I ATCGAT 1 cut(s) 414
Bsu36I CCTNAGG 1 cut(s) 2196
BsuRI GGCC 8 cut(s) 294, 834, 1337, 1634, 4143, 5129, 5346, 5358
BsuTUI ATCGAT 1 cut(s) 414
BtgI CCRYGG 3 cut(s) 514, 2274, 4138
BtgZI GCGATG 2 cut(s) 1506, 4491
BtsCI GGATG 9 cut(s) 727, 1527, 2473, 3301, 3440, 3606, 3665, 4285, 5239
BtsI GCAGTG 1 cut(s) 2504
BtsIMutI CAGTG 9 cut(s) 288, 867, 897, 1258, 2504, 2671, 3253, 3774, 3985
BveI ACCTGC 4 cut(s) 322, 1949, 2356, 3190
Cac8I GCNNGC 9 cut(s) 99, 243, 527, 1048, 1679, 2049, 2655, 3900, 5131
CaiI CAGNNNCTG 1 cut(s) 619
CciI TCATGA 1 cut(s) 4291
CfoI GCGC 8 cut(s) 346, 1066, 1090, 1677, 1842, 2213, 3358, 4106
Cfr13I GGNCC 7 cut(s) 206, 370, 1730, 3062, 4901, 5127, 5357
ClaI ATCGAT 1 cut(s) 414
CseI GACGC 7 cut(s) 63, 742, 926, 998, 1965, 1982, 4304
CspCI CAANNNNNGTGG 4 cut(s) 265, 300, 2968, 3003
DinI GGCGCC 1 cut(s) 1089
DraI TTTAAA 2 cut(s) 406, 4530
DrdI GACNNNNNNGTC 1 cut(s) 1737
DriI GACNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 1737
EaeI YGGCCR 2 cut(s) 1632, 4141
Eam1104I CTCTTC 4 cut(s) 2874, 4665, 5019, 5167
Eam1105I GACNNNNNGTC 1 cut(s) 184
EarI CTCTTC 4 cut(s) 2874, 4665, 5019, 5167
EciI GGCGGA 3 cut(s) 662, 3144, 3470
Ecl136II GAGCTC 2 cut(s) 691, 5192
Eco105I TACGTA 1 cut(s) 4210
Eco130I CCWWGG 4 cut(s) 514, 597, 2274, 4138
Eco24I GRGCYC 3 cut(s) 693, 2647, 5194
Eco31I GGTCTC 4 cut(s) 1538, 3312, 4304, 4414
Eco32I GATATC 3 cut(s) 1216, 2827, 3853
Eco47I GGWCC 5 cut(s) 206, 370, 1730, 3062, 4901
Eco53kI GAGCTC 2 cut(s) 691, 5192
Eco57I CTGAAG 5 cut(s) 247, 1788, 1847, 1968, 4518
Eco72I CACGTG 1 cut(s) 3638
Eco81I CCTNAGG 1 cut(s) 2196
Eco88I CYCGRG 1 cut(s) 1856
EcoICRI GAGCTC 2 cut(s) 691, 5192
EcoO109I RGGNCCY 3 cut(s) 1730, 3062, 4901
EcoRII CCWGG 7 cut(s) 1050, 1561, 2128, 2903, 3064, 4298, 4752
EcoRV GATATC 3 cut(s) 1216, 2827, 3853
EcoT14I CCWWGG 4 cut(s) 514, 597, 2274, 4138
EcoT22I ATGCAT 3 cut(s) 2049, 2053, 4828
EcoT38I GRGCYC 3 cut(s) 693, 2647, 5194
EgeI GGCGCC 1 cut(s) 1089
EheI GGCGCC 1 cut(s) 1089
ErhI CCWWGG 4 cut(s) 514, 597, 2274, 4138
Esp3I CGTCTC 1 cut(s) 981
FalI AAGNNNNNCTT 2 cut(s) 3897, 3929
FaqI GGGAC 5 cut(s) 376, 739, 865, 1725, 1743
FauI CCCGC 1 cut(s) 1918
FauNDI CATATG 7 cut(s) 847, 2053, 2512, 2544, 2708, 3109, 3697
FbaI TGATCA 4 cut(s) 273, 2244, 2700, 4252
FokI GGATG 9 cut(s) 734, 1534, 2480, 3308, 3447, 3593, 3672, 4292, 5246
FriOI GRGCYC 3 cut(s) 693, 2647, 5194
GlaI GCGC 8 cut(s) 345, 1065, 1089, 1676, 1841, 2212, 3357, 4105
GsaI CCCAGC 1 cut(s) 2822
GsuI CTGGAG 2 cut(s) 1197, 2955
HaeII RGCGCY 3 cut(s) 1067, 1091, 1843
HaeIII GGCC 8 cut(s) 294, 834, 1337, 1634, 4143, 5129, 5346, 5358
HapII CCGG 8 cut(s) 204, 378, 634, 1321, 1734, 1843, 3880, 5360
HgaI GACGC 7 cut(s) 63, 742, 926, 998, 1965, 1982, 4304
HhaI GCGC 8 cut(s) 346, 1066, 1090, 1677, 1842, 2213, 3358, 4106
Hin1I GRCGYC 2 cut(s) 1088, 4296
Hin6I GCGC 8 cut(s) 344, 1064, 1088, 1675, 1840, 2211, 3356, 4104
HinP1I GCGC 8 cut(s) 344, 1064, 1088, 1675, 1840, 2211, 3356, 4104
HincII GTYRAC 3 cut(s) 657, 2586, 5050
HindII GTYRAC 3 cut(s) 657, 2586, 5050
HindIII AAGCTT 2 cut(s) 3896, 4408
HpaII CCGG 8 cut(s) 204, 378, 634, 1321, 1734, 1843, 3880, 5360
Hpy166II GTNNAC 9 cut(s) 86, 209, 657, 2205, 2586, 2802, 4018, 5050, 5226
Hpy8I GTNNAC 9 cut(s) 86, 209, 657, 2205, 2586, 2802, 4018, 5050, 5226
Hpy99I CGWCG 3 cut(s) 79, 221, 2336
HpyCH4IV ACGT 7 cut(s) 576, 1710, 2176, 2419, 3147, 3637, 4209
HpySE526I ACGT 7 cut(s) 576, 1710, 2176, 2419, 3147, 3637, 4209
Hsp92I GRCGYC 2 cut(s) 1088, 4296
HspAI GCGC 8 cut(s) 344, 1064, 1088, 1675, 1840, 2211, 3356, 4104
KasI GGCGCC 1 cut(s) 1087
KflI GGGWCCC 1 cut(s) 1730
Kpn2I TCCGGA 1 cut(s) 633
Ksp22I TGATCA 4 cut(s) 273, 2244, 2700, 4252
MaeII ACGT 7 cut(s) 576, 1710, 2176, 2419, 3147, 3637, 4209
MaeIII GTNAC 7 cut(s) 40, 350, 2177, 2782, 3424, 3633, 4865
MbiI CCGCTC 2 cut(s) 127, 386
MflI RGATCY 6 cut(s) 510, 1111, 1158, 3910, 4342, 4921
MhlI GDGCHC 5 cut(s) 693, 2647, 2968, 4612, 5194
MlsI TGGCCA 2 cut(s) 1634, 4143
MluI ACGCGT 3 cut(s) 72, 1974, 3322
MluNI TGGCCA 2 cut(s) 1634, 4143
Mly113I GGCGCC 1 cut(s) 1088
MmeI TCCRAC 5 cut(s) 870, 894, 1146, 3174, 4673
Mox20I TGGCCA 2 cut(s) 1634, 4143
Mph1103I ATGCAT 3 cut(s) 2049, 2053, 4828
MroI TCCGGA 1 cut(s) 633
MroXI GAANNNNTTC 2 cut(s) 3136, 5040
MscI TGGCCA 2 cut(s) 1634, 4143
MslI CAYNNNNRTG 4 cut(s) 2052, 2772, 3566, 3674
Msp20I TGGCCA 2 cut(s) 1634, 4143
MspA1I CMGCKG 4 cut(s) 1211, 2822, 3692, 4733
MspI CCGG 8 cut(s) 204, 378, 634, 1321, 1734, 1843, 3880, 5360
Mva1269I GAATGC 5 cut(s) 113, 327, 2460, 2660, 3824
MvaI CCWGG 7 cut(s) 1052, 1563, 2130, 2905, 3066, 4300, 4754
MvnI CGCG 4 cut(s) 74, 344, 1976, 3324
NarI GGCGCC 1 cut(s) 1088
NciI CCSGG 5 cut(s) 204, 1322, 1734, 1844, 5360
NcoI CCATGG 3 cut(s) 514, 2274, 4138
NdeI CATATG 7 cut(s) 847, 2053, 2512, 2544, 2708, 3109, 3697
NheI GCTAGC 1 cut(s) 97
NmeAIII GCCGAG 1 cut(s) 4751
NmuCI GTSAC 3 cut(s) 2782, 3424, 3633
NsiI ATGCAT 3 cut(s) 2049, 2053, 4828
NspI RCATGY 6 cut(s) 913, 2051, 3673, 4780, 4850, 5341
PaeI GCATGC 1 cut(s) 2051
PaeR7I CTCGAG 1 cut(s) 1856
PagI TCATGA 1 cut(s) 4291
PciI ACATGT 2 cut(s) 909, 4776
PcsI WCGNNNNNNNCGW 1 cut(s) 5310
PctI GAATGC 5 cut(s) 113, 327, 2460, 2660, 3824
PdmI GAANNNNTTC 2 cut(s) 3136, 5040
Pfl23II CGTACG 1 cut(s) 4210
PflFI GACNNNGTC 1 cut(s) 799
PflMI CCANNNNNTGG 2 cut(s) 439, 619
Ple19I CGATCG 1 cut(s) 231
PluTI GGCGCC 1 cut(s) 1091
PmaCI CACGTG 1 cut(s) 3638
PmlI CACGTG 1 cut(s) 3638
Ppu21I YACGTR 2 cut(s) 3638, 4210
PpuMI RGGWCCY 3 cut(s) 1730, 3062, 4901
PscI ACATGT 2 cut(s) 909, 4776
PsiI TTATAA 1 cut(s) 3363
Psp124BI GAGCTC 2 cut(s) 693, 5194
Psp1406I AACGTT 1 cut(s) 2176
Psp5II RGGWCCY 3 cut(s) 1730, 3062, 4901
Psp6I CCWGG 7 cut(s) 1050, 1561, 2128, 2903, 3064, 4298, 4752
PspCI CACGTG 1 cut(s) 3638
PspFI CCCAGC 1 cut(s) 2818
PspGI CCWGG 7 cut(s) 1050, 1561, 2128, 2903, 3064, 4298, 4752
PspLI CGTACG 1 cut(s) 4210
PspPI GGNCC 7 cut(s) 206, 370, 1730, 3062, 4901, 5127, 5357
PspPPI RGGWCCY 3 cut(s) 1730, 3062, 4901
PspXI VCTCGAGB 1 cut(s) 1856
PstI CTGCAG 1 cut(s) 144
PstNI CAGNNNCTG 1 cut(s) 619
PsuI RGATCY 6 cut(s) 510, 1111, 1158, 3910, 4342, 4921
PsyI GACNNNGTC 1 cut(s) 799
PvuI CGATCG 1 cut(s) 231
PvuII CAGCTG 2 cut(s) 1211, 2822
RseI CAYNNNNRTG 4 cut(s) 2052, 2772, 3566, 3674
SacI GAGCTC 2 cut(s) 693, 5194
Sau96I GGNCC 7 cut(s) 206, 370, 1730, 3062, 4901, 5127, 5357
ScaI AGTACT 3 cut(s) 236, 926, 4392
SduI GDGCHC 5 cut(s) 693, 2647, 2968, 4612, 5194
SfcI CTRYAG 7 cut(s) 140, 2601, 3087, 3867, 4109, 4328, 4365
SfoI GGCGCC 1 cut(s) 1089
Sfr274I CTCGAG 1 cut(s) 1856
SinI GGWCC 5 cut(s) 206, 370, 1730, 3062, 4901
SlaI CTCGAG 1 cut(s) 1856
SmiMI CAYNNNNRTG 4 cut(s) 2052, 2772, 3566, 3674
SmlI CTYRAG 5 cut(s) 1145, 1548, 1856, 2036, 3097
SmoI CTYRAG 5 cut(s) 1145, 1548, 1856, 2036, 3097
SnaBI TACGTA 1 cut(s) 4210
SphI GCATGC 1 cut(s) 2051
SspDI GGCGCC 1 cut(s) 1087
SspI AATATT 4 cut(s) 2842, 3462, 3715, 3761
SstI GAGCTC 2 cut(s) 693, 5194
StyI CCWWGG 4 cut(s) 514, 597, 2274, 4138
TaiI ACGT 7 cut(s) 579, 1713, 2179, 2422, 3150, 3640, 4212
TaqII GACCGA 1 cut(s) 2166
TatI WGTACW 7 cut(s) 234, 924, 942, 4316, 4390, 4779, 5225
TauI GCSGC 6 cut(s) 130, 1093, 2674, 3695, 4541, 4733
TscAI CASTG 9 cut(s) 295, 867, 904, 1258, 2504, 2671, 3253, 3781, 3985
TseFI GTSAC 3 cut(s) 2782, 3424, 3633
Tsp45I GTSAC 3 cut(s) 2782, 3424, 3633
TspGWI ACGGA 9 cut(s) 409, 795, 896, 999, 1794, 2349, 2480, 4020, 4869
TspRI CASTG 9 cut(s) 295, 867, 904, 1258, 2504, 2671, 3253, 3781, 3985
Tth111I GACNNNGTC 1 cut(s) 799
Van91I CCANNNNNTGG 2 cut(s) 439, 619
VpaK11BI GGWCC 5 cut(s) 206, 370, 1730, 3062, 4901
XapI RAATTY 6 cut(s) 133, 2084, 3181, 3497, 3542, 4355
XbaI TCTAGA 2 cut(s) 955, 4381
XceI RCATGY 6 cut(s) 913, 2051, 3673, 4780, 4850, 5341
XcmI CCANNNNNNNNNTGG 1 cut(s) 1751
XhoI CTCGAG 1 cut(s) 1856
XmnI GAANNNNTTC 2 cut(s) 3136, 5040
ZrmI AGTACT 3 cut(s) 236, 926, 4392
Zsp2I ATGCAT 3 cut(s) 2049, 2053, 4828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.