pycom02g16550

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007735
Physical Location & Seq
Reverse (-)
13702434 .. 13707052
4619 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g16550.2

Sequence Viewer

Length: 2811 bp
ATGCAAGAACAATTTAAAGAAAAAGCTCGCAAATCTGAGTCAGTTTATTATGCTGCAACAGATGTGATAATTCTTAGATTCATGGTTGAGGTATGCTGGGCCCCTATGCTGGCTGCCTTCAGTGTTCCACTTGATCAAAGTGACGATGAAGTAGTTATTTCACTGTGTCTGGAAGGCTTTCGTCATGCCATTCATGTTACTGCTGTAATGTCCATGAAGACCCATAGAGACGCTTTTGTGACTTCACTAGCAAAGTTTACTTCTCTCCACTCTCCTGCCGATATCAAGCAGAAGAATATTGACGCAATTAAGGCAATAGTGACAATTGCAGATGAAGATGGAAACTACCTACAAGAAGCTTGGGAACATATTTTGACTTGTGTTTCCCGGTTTGAACATCTACATCTCTTGGGAGAGGGTGCTCCTCCAGATGCTACGTTCTTTGCCTTTCCTCAGAGTGAGTCAGAAAAAACAAAACAGACTAAGTCCACTATCCTTCCTGTTTTAAAAAAGAAGGGGCAAGGAAGGATGCAGTATGCAGCTGCTGCTGTGTTGCGAGGTTCATATGATAGTGCTGGTATTGGTGGGAATGCTTCTGGGGCTGTCACATCAGAACAGATGAACAATTTGGTTTCTAATTTAAACATGCTAGAACAAGTTGGTGAAGTGAGCCGCATATTCACACGGAGTCAGAAACTAAACAGTGAGGCGATTGTGGACTTTGTTAAAGCACTTTGCAAGGTCTCTATGGAAGAATTGCGATCGGCCTCTGATCCTCGGGTTTTCAGCCTTACAAAGATTGTTGAGATTGCGCATTATAATATGAACCGCATCAGGCTTGTCTGGTCTAGCATCTGGCATGTGCTCTCTAATTTCTTTGTGACAATTGGCTGTTCTGAAAACCTGTCAATCGCCATTTTTGCTATGGATTCTCTACGCCAGTTGTCAATGAAATTCTTGGACCGAGAAGAGTTGGCTAATTATAATTTTCAGAATGAGTTCATGAAACCTTTTGTCATTGTTATGCGTAAAAGCAGTGCAGTTGAAATACGTGAATTAATCATCAGATGTGTCTCACAAATGGTATTATCTCGTGTCAACAATGTCAAATCTGGATGGAAGAGCATGTTCATGGTATTCACTACTGCAGCTTATGACGACCACAAAAACATTGTACTCTTGGCCTTTGAAATAATTGAGAAGATTATTCGAGACTATTTTCCATACATTACTGAGACTGAAACCACTACCTTTACTGATTGCGTGAATTGCCTAATTGCATTTACGAATAGTAGATTCAACAAGGACATTAGCCTCAATGCTATTGCTTTTCTTCAATTCTGTGCTACAAAACTTGCAGATGGAGGTCTTGGTTCCTCATCAAAAAATAAGGACAAGGAAGCTTCTGGAAAAATCTCGCCATCTTCACCTCAAGCCTGGAAAGATGGAAAACAAGAAAATGGTGAAATGCCAGATAAGGATGACCATATCTATTTCTGGTTCCCTTTATTGGCTGGTTTATCCGAGCTTAGCTTTGACCCTAGGCCTGAAATCAGGAGGAGTGCCCTACAAGTTCTGTTTGAAACTTTACGTAACCATGGTCACCTTTTCTCACTACCTTTGTGGGAAAGGGTGTTTGAATCAGTGCTATTTCCAATATTTGACTATGTGCGGCATGCTATCGATCCATCTGGGGAAGGTTCACCAGGGCAAGGGACTGATGGTGATGTGGGTGATCTTGATCAAGATGCCTGGCTCTATGAGACATGCACATTGGCACTTCAATTAGTTGTTGATCTCTTTGTCAAATTTTATAACACTGTCAATCCGCTATTGAAGAAAGTTTTGGTGCTTCTAGTCAGTTTTATTCGACGACCACACCAAAGCTTAGCTGGTATTGGTATTGCTGCATTTGTGCGATTGATGAGCAATGCAGGGGATCTCTATTCTCATGAAAAGTGGCTAGAAGTGGTTTCATCATTAAAAGAAGCAGCCAATTCAACGCTTCCTGATTTCTCTTTCCTTAGTGGAGATGGCATCATTAGAAGCCATGAACATGCTTTGAGCAGAGAAGAGAATGGAGAGTCCACCGTGTCTGGCAGGAGTGATGAGGATTCAGAGAGACTGAGGACAAACCACATGTATGCTGGAATATCTGACGTCAAGTGCCGAGCTGCTGTTCAACTTTTATTGATTCAGGCGGTGATGGAGATTTACACCATGTATCGCTCTCACCTTTCCACGAGAAACACATTAGTCCTTTTTGATGCTTTGCACGATGTTGCATCTCACGCTCACAAGATTAACACCGATGCCACTTTAAGAGCAAGGTTACAAGAATTTGGTGCCGTGACCCAAATGCAAGACCCTCCACTGTTGCGCATCGAGAATGAATCTTACCAAATATGCCTTACATTCCTACAGAACCTTGTAGAGGATAGCCCAGCAGGTTACAATGAAGTGGAGGTGGAGTCCTGCATCGTGGACCTCTGCAGAGAGGTTTTACAGTTTTATATTGAAGCTGCTAGCTCTGGAAAGGTATCTGAATCATCCAAAGGGCAGCAGCTCCAGTGGCTAATTCCTCTAGGTTCTGGGAGACGAAGAGAATTGGCCCAACGTGCCCCGCTTATGGTGGCAACTCTCCAGACTATCTGTAGTTTGGGAGAGTCCTCATTTGAAAACAACTTGTCTCAGTTCTTCCCCCTCCTTTCCAGCTTGATAAGTTGTGAACACGGTTCAAATGAGGTCCAGATTGCCCTCGGTGACATGCTCAGCTCATCTGTGGGGCCTGTGTTGCTTCGGTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

937

Amino Acids

104.74

Weight (kDa)

5.58

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 819, 984, 1815
AatII GACGTC 1 cut(s) 2163
Acc16I TGCGCA 2 cut(s) 813, 2381
Acc36I ACCTGC 1 cut(s) 2438
AccB1I GGYRCC 1 cut(s) 2345
AciI CCGC 6 cut(s) 673, 829, 1672, 1829, 2201, 2624
AclWI GGATC 3 cut(s) 767, 1679, 1947
AcsI RAATTY 3 cut(s) 953, 1808, 2339
AcuI CTGAAG 1 cut(s) 103
AcyI GRCGYC 1 cut(s) 2160
AfaI GTAC 1 cut(s) 1176
AfiI CCNNNNNNNGG 5 cut(s) 109, 1510, 1712, 2434, 2629
AflIII ACRYGT 1 cut(s) 2139
AjnI CCWGG 3 cut(s) 1436, 1705, 1751
AjuI GAANNNNNNNTTGG 2 cut(s) 1829, 1861
Alw21I GWGCWC 2 cut(s) 424, 867
Alw26I GTCTC 9 cut(s) 222, 748, 1078, 1206, 1229, 1757, 2116, 2590, 2694
AlwI GGATC 3 cut(s) 767, 1679, 1947
AlwNI CAGNNNCTG 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 777
AoxI GGCC 6 cut(s) 99, 765, 1182, 1544, 2610, 2786
ApaI GGGCCC 1 cut(s) 103
ApoI RAATTY 3 cut(s) 953, 1808, 2339
AseI ATTAAT 1 cut(s) 1058
Asp700I GAANNNNTTC 2 cut(s) 177, 998
AspA2I CCTAGG 1 cut(s) 1541
AspLEI GCGC 2 cut(s) 814, 2382
AspS9I GGNCC 7 cut(s) 99, 100, 961, 2485, 2611, 2746, 2786
AsuC2I CCSGG 1 cut(s) 388
AsuNHI GCTAGC 1 cut(s) 2525
AvaI CYCGRG 1 cut(s) 777
AvaII GGWCC 3 cut(s) 961, 2485, 2746
AvrII CCTAGG 1 cut(s) 1541
BaeGI GKGCMC 3 cut(s) 103, 1567, 2623
BanI GGYRCC 1 cut(s) 2345
BanII GRGCYC 1 cut(s) 103
BauI CACGAG 2 cut(s) 1092, 2242
BbsI GAAGAC 1 cut(s) 224
Bbv12I GWGCWC 2 cut(s) 424, 867
BccI CCATC 9 cut(s) 332, 1110, 1355, 1429, 1439, 1696, 1715, 2027, 2200
BceAI ACGGC 1 cut(s) 2333
BciT130I CCWGG 3 cut(s) 1438, 1707, 1753
BclI TGATCA 2 cut(s) 133, 1741
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 9 cut(s) 222, 748, 1078, 1206, 1229, 1757, 2116, 2590, 2694
BfaI CTAG 8 cut(s) 248, 650, 849, 1542, 1856, 1964, 2526, 2585
BfmI CTRYAG 4 cut(s) 1146, 2420, 2491, 2653
BfuAI ACCTGC 1 cut(s) 2438
BlnI CCTAGG 1 cut(s) 1541
BlpI GCTNAGC 3 cut(s) 1529, 1888, 2771
Bme1390I CCNGG 4 cut(s) 388, 1438, 1707, 1753
Bme18I GGWCC 3 cut(s) 961, 2485, 2746
BmeT110I CYCGRG 1 cut(s) 777
BmgT120I GGNCC 7 cut(s) 99, 100, 961, 2485, 2611, 2746, 2786
BmiI GGNNCC 6 cut(s) 101, 102, 1375, 1502, 2347, 2787
BmrFI CCNGG 4 cut(s) 388, 1438, 1707, 1753
BmtI GCTAGC 1 cut(s) 2529
BpiI GAAGAC 1 cut(s) 224
BpmI CTGGAG 3 cut(s) 411, 2552, 2627
Bpu1102I GCTNAGC 3 cut(s) 1529, 1888, 2771
BpuEI CTTGAG 1 cut(s) 1416
BpuMI CCSGG 1 cut(s) 388
Bsa29I ATCGAT 1 cut(s) 1683
BsaAI YACGTR 2 cut(s) 1052, 1592
BsaBI GATNNNNATC 1 cut(s) 1740
BsaHI GRCGYC 1 cut(s) 2160
BsaI GGTCTC 1 cut(s) 748
BsaJI CCNNGG 5 cut(s) 776, 1541, 1597, 1706, 2758
BsaXI ACNNNNNCTCC 2 cut(s) 2456, 2486
Bsc4I CCNNNNNNNGG 5 cut(s) 109, 1510, 1712, 2434, 2629
Bse1I ACTGG 2 cut(s) 940, 2569
Bse3DI GCAATG 1 cut(s) 1936
Bse8I GATNNNNATC 1 cut(s) 1740
BseBI CCWGG 3 cut(s) 1438, 1707, 1753
BseCI ATCGAT 1 cut(s) 1683
BseDI CCNNGG 5 cut(s) 776, 1541, 1597, 1706, 2758
BseGI GGATG 4 cut(s) 534, 1121, 1486, 2549
BseJI GATNNNNATC 1 cut(s) 1740
BseLI CCNNNNNNNGG 5 cut(s) 109, 1510, 1712, 2434, 2629
BseMI GCAATG 1 cut(s) 1936
BseMII CTCAG 6 cut(s) 27, 467, 1224, 2117, 2705, 2785
BseNI ACTGG 2 cut(s) 940, 2569
BseRI GAGGAG 2 cut(s) 414, 1573
BseSI GKGCMC 3 cut(s) 103, 1567, 2623
BseYI CCCAGC 2 cut(s) 96, 2443
BsgI GTGCAG 1 cut(s) 1059
Bsh1285I CGRYCG 2 cut(s) 764, 2804
BshFI GGCC 6 cut(s) 101, 767, 1184, 1546, 2612, 2788
BshNI GGYRCC 1 cut(s) 2345
BshVI ATCGAT 1 cut(s) 1683
BsiEI CGRYCG 2 cut(s) 764, 2804
BsiHKAI GWGCWC 2 cut(s) 424, 867
BsiHKCI CYCGRG 1 cut(s) 777
BsiSI CCGG 1 cut(s) 388
BslFI GGGAC 1 cut(s) 1729
BslI CCNNNNNNNGG 5 cut(s) 109, 1510, 1712, 2434, 2629
BsmAI GTCTC 9 cut(s) 222, 748, 1078, 1206, 1229, 1757, 2116, 2590, 2694
BsmBI CGTCTC 2 cut(s) 222, 2590
BsmFI GGGAC 1 cut(s) 1729
BsmI GAATGC 1 cut(s) 595
BsnI GGCC 6 cut(s) 101, 767, 1184, 1546, 2612, 2788
Bso31I GGTCTC 1 cut(s) 748
BsoBI CYCGRG 1 cut(s) 777
Bsp120I GGGCCC 1 cut(s) 99
Bsp1286I GDGCHC 5 cut(s) 103, 424, 867, 1567, 2623
Bsp143I GATC 8 cut(s) 133, 761, 772, 1684, 1735, 1741, 1795, 1939
Bsp1720I GCTNAGC 3 cut(s) 1529, 1888, 2771
Bsp19I CCATGG 1 cut(s) 1597
BspACI CCGC 6 cut(s) 673, 829, 1672, 1829, 2201, 2624
BspANI GGCC 6 cut(s) 101, 767, 1184, 1546, 2612, 2788
BspCNI CTCAG 6 cut(s) 28, 466, 1225, 2118, 2704, 2784
BspDI ATCGAT 1 cut(s) 1683
BspHI TCATGA 2 cut(s) 1002, 1951
BspLI GGNNCC 6 cut(s) 101, 102, 1375, 1502, 2347, 2787
BspMAI CTGCAG 2 cut(s) 1150, 2495
BspMI ACCTGC 1 cut(s) 2438
BspOI GCTAGC 1 cut(s) 2529
BspPI GGATC 3 cut(s) 767, 1679, 1947
BspQI GCTCTTC 1 cut(s) 1115
BspT107I GGYRCC 1 cut(s) 2345
BspTNI GGTCTC 1 cut(s) 748
BsrDI GCAATG 1 cut(s) 1936
BsrI ACTGG 2 cut(s) 940, 2569
BssECI CCNNGG 5 cut(s) 776, 1541, 1597, 1706, 2758
BssMI GATC 8 cut(s) 133, 761, 772, 1684, 1735, 1741, 1795, 1939
BssNI GRCGYC 1 cut(s) 2160
BssSI CACGAG 2 cut(s) 1092, 2242
BssT1I CCWWGG 2 cut(s) 1541, 1597
Bst2BI CACGAG 2 cut(s) 1092, 2242
Bst2UI CCWGG 3 cut(s) 1438, 1707, 1753
Bst4CI ACNGT 7 cut(s) 165, 704, 1822, 2092, 2376, 2508, 2735
Bst6I CTCTTC 4 cut(s) 963, 1115, 2067, 2596
BstACI GRCGYC 1 cut(s) 2160
BstAPI GCANNNNNTGC 1 cut(s) 545
BstBAI YACGTR 2 cut(s) 1052, 1592
BstC8I GCNNGC 4 cut(s) 28, 111, 1677, 2527
BstDSI CCRYGG 1 cut(s) 1597
BstEII GGTNACC 1 cut(s) 1601
BstENI CCTNNNNNAGG 1 cut(s) 2432
BstF5I GGATG 4 cut(s) 534, 1121, 1486, 2549
BstHHI GCGC 2 cut(s) 814, 2382
BstKTI GATC 8 cut(s) 136, 764, 775, 1687, 1738, 1744, 1798, 1942
BstMAI GTCTC 9 cut(s) 222, 748, 1078, 1206, 1229, 1757, 2116, 2590, 2694
BstMBI GATC 8 cut(s) 133, 761, 772, 1684, 1735, 1741, 1795, 1939
BstMCI CGRYCG 2 cut(s) 764, 2804
BstMWI GCNNNNNNNGC 9 cut(s) 311, 545, 599, 920, 1269, 2291, 2572, 2618, 2794
BstNI CCWGG 3 cut(s) 1438, 1707, 1753
BstNSI RCATGY 8 cut(s) 649, 863, 1129, 1679, 1770, 2060, 2143, 2770
BstPI GGTNACC 1 cut(s) 1601
BstSCI CCNGG 4 cut(s) 386, 1436, 1705, 1751
BstSFI CTRYAG 4 cut(s) 1146, 2420, 2491, 2653
BstSLI GKGCMC 3 cut(s) 103, 1567, 2623
BstSNI TACGTA 1 cut(s) 1592
BstV2I GAAGAC 1 cut(s) 224
BstX2I RGATCY 1 cut(s) 1939
BstYI RGATCY 1 cut(s) 1939
Bsu15I ATCGAT 1 cut(s) 1683
BsuRI GGCC 6 cut(s) 101, 767, 1184, 1546, 2612, 2788
BsuTUI ATCGAT 1 cut(s) 1683
BtgI CCRYGG 1 cut(s) 1597
BtsCI GGATG 4 cut(s) 534, 1121, 1486, 2549
BtsI GCAGTG 1 cut(s) 1042
BtsIMutI CAGTG 8 cut(s) 127, 161, 709, 1042, 1650, 1818, 2372, 2576
BveI ACCTGC 1 cut(s) 2438
Cac8I GCNNGC 4 cut(s) 28, 111, 1677, 2527
CaiI CAGNNNCTG 1 cut(s) 545
CciI TCATGA 2 cut(s) 1002, 1951
CfoI GCGC 2 cut(s) 814, 2382
Cfr13I GGNCC 7 cut(s) 99, 100, 961, 2485, 2611, 2746, 2786
ClaI ATCGAT 1 cut(s) 1683
CseI GACGC 2 cut(s) 239, 311
Csp6I GTAC 1 cut(s) 1175
CviQI GTAC 1 cut(s) 1175
DpnI GATC 8 cut(s) 135, 763, 774, 1686, 1737, 1743, 1797, 1941
DpnII GATC 8 cut(s) 133, 761, 772, 1684, 1735, 1741, 1795, 1939
DraI TTTAAA 3 cut(s) 16, 507, 642
Eam1104I CTCTTC 4 cut(s) 963, 1115, 2067, 2596
EarI CTCTTC 4 cut(s) 963, 1115, 2067, 2596
Eco105I TACGTA 1 cut(s) 1592
Eco130I CCWWGG 2 cut(s) 1541, 1597
Eco147I AGGCCT 1 cut(s) 1546
Eco24I GRGCYC 1 cut(s) 103
Eco31I GGTCTC 1 cut(s) 748
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 3 cut(s) 961, 2485, 2746
Eco57I CTGAAG 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 777
Eco91I GGTNACC 1 cut(s) 1601
EcoNI CCTNNNNNAGG 1 cut(s) 2432
EcoO109I RGGNCCY 2 cut(s) 100, 2786
EcoO65I GGTNACC 1 cut(s) 1601
EcoRII CCWGG 3 cut(s) 1436, 1705, 1751
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 2 cut(s) 1541, 1597
EcoT38I GRGCYC 1 cut(s) 103
ErhI CCWWGG 2 cut(s) 1541, 1597
Esp3I CGTCTC 2 cut(s) 222, 2590
FaqI GGGAC 1 cut(s) 1729
FauI CCCGC 1 cut(s) 2631
FauNDI CATATG 1 cut(s) 565
FbaI TGATCA 2 cut(s) 133, 1741
FokI GGATG 4 cut(s) 541, 1128, 1493, 2536
FriOI GRGCYC 1 cut(s) 103
FspBI CTAG 8 cut(s) 248, 650, 849, 1542, 1856, 1964, 2526, 2585
FspI TGCGCA 2 cut(s) 813, 2381
GlaI GCGC 2 cut(s) 813, 2381
GsaI CCCAGC 2 cut(s) 100, 2447
GsuI CTGGAG 3 cut(s) 411, 2552, 2627
HaeIII GGCC 6 cut(s) 101, 767, 1184, 1546, 2612, 2788
HapII CCGG 1 cut(s) 388
HgaI GACGC 2 cut(s) 239, 311
HhaI GCGC 2 cut(s) 814, 2382
Hin1I GRCGYC 1 cut(s) 2160
Hin6I GCGC 2 cut(s) 812, 2380
HinP1I GCGC 2 cut(s) 812, 2380
HincII GTYRAC 1 cut(s) 1099
HindII GTYRAC 1 cut(s) 1099
HindIII AAGCTT 3 cut(s) 357, 1401, 1885
HpaII CCGG 1 cut(s) 388
Hpy166II GTNNAC 8 cut(s) 258, 489, 718, 1099, 1703, 2088, 2485, 2729
Hpy8I GTNNAC 8 cut(s) 258, 489, 718, 1099, 1703, 2088, 2485, 2729
Hpy99I CGWCG 1 cut(s) 1875
HpyAV CCTTC 6 cut(s) 127, 167, 506, 508, 519, 1691
HpyCH4III ACNGT 7 cut(s) 165, 704, 1822, 2092, 2376, 2508, 2735
HpyCH4IV ACGT 5 cut(s) 437, 1051, 1591, 2160, 2617
HpyF10VI GCNNNNNNNGC 9 cut(s) 311, 545, 599, 920, 1269, 2291, 2572, 2618, 2794
HpySE526I ACGT 5 cut(s) 437, 1051, 1591, 2160, 2617
Hsp92I GRCGYC 1 cut(s) 2160
HspAI GCGC 2 cut(s) 812, 2380
Ksp22I TGATCA 2 cut(s) 133, 1741
Kzo9I GATC 8 cut(s) 133, 761, 772, 1684, 1735, 1741, 1795, 1939
LguI GCTCTTC 1 cut(s) 1115
LmnI GCTCC 2 cut(s) 427, 2571
MaeI CTAG 8 cut(s) 248, 650, 849, 1542, 1856, 1964, 2526, 2585
MaeII ACGT 5 cut(s) 437, 1051, 1591, 2160, 2617
MalI GATC 8 cut(s) 135, 763, 774, 1686, 1737, 1743, 1797, 1941
MboI GATC 8 cut(s) 133, 761, 772, 1684, 1735, 1741, 1795, 1939
MfeI CAATTG 2 cut(s) 324, 885
MflI RGATCY 1 cut(s) 1939
MhlI GDGCHC 5 cut(s) 103, 424, 867, 1567, 2623
MlyI GAGTC 6 cut(s) 47, 470, 697, 2093, 2480, 2675
MroXI GAANNNNTTC 2 cut(s) 177, 998
MseI TTAA 9 cut(s) 15, 309, 506, 641, 726, 1058, 1982, 2304, 2321
MslI CAYNNNNRTG 4 cut(s) 1022, 1130, 2055, 2142
MspA1I CMGCKG 1 cut(s) 542
MspI CCGG 1 cut(s) 388
MspR9I CCNGG 4 cut(s) 388, 1438, 1707, 1753
MunI CAATTG 2 cut(s) 324, 885
Mva1269I GAATGC 1 cut(s) 595
MvaI CCWGG 3 cut(s) 1438, 1707, 1753
MwoI GCNNNNNNNGC 9 cut(s) 311, 545, 599, 920, 1269, 2291, 2572, 2618, 2794
NciI CCSGG 1 cut(s) 388
NcoI CCATGG 1 cut(s) 1597
NdeI CATATG 1 cut(s) 565
NdeII GATC 8 cut(s) 133, 761, 772, 1684, 1735, 1741, 1795, 1939
NheI GCTAGC 1 cut(s) 2525
NlaIV GGNNCC 6 cut(s) 101, 102, 1375, 1502, 2347, 2787
NmeAIII GCCGAG 1 cut(s) 2195
NmuCI GTSAC 8 cut(s) 140, 238, 319, 604, 880, 1601, 2350, 2762
NsbI TGCGCA 2 cut(s) 813, 2381
NspI RCATGY 8 cut(s) 649, 863, 1129, 1679, 1770, 2060, 2143, 2770
PaeI GCATGC 1 cut(s) 1679
PagI TCATGA 2 cut(s) 1002, 1951
PceI AGGCCT 1 cut(s) 1546
PciI ACATGT 1 cut(s) 2139
PciSI GCTCTTC 1 cut(s) 1115
PctI GAATGC 1 cut(s) 595
PdmI GAANNNNTTC 2 cut(s) 177, 998
PfeI GAWTC 8 cut(s) 78, 929, 1296, 1640, 2114, 2194, 2393, 2546
PflFI GACNNNGTC 1 cut(s) 484
Ple19I CGATCG 1 cut(s) 764
PleI GAGTC 6 cut(s) 46, 469, 696, 2092, 2479, 2674
PpsI GAGTC 6 cut(s) 46, 469, 696, 2092, 2479, 2674
Ppu21I YACGTR 2 cut(s) 1052, 1592
PscI ACATGT 1 cut(s) 2139
PshBI ATTAAT 1 cut(s) 1058
PsiI TTATAA 3 cut(s) 819, 984, 1815
Psp6I CCWGG 3 cut(s) 1436, 1705, 1751
PspEI GGTNACC 1 cut(s) 1601
PspFI CCCAGC 2 cut(s) 96, 2443
PspGI CCWGG 3 cut(s) 1436, 1705, 1751
PspN4I GGNNCC 6 cut(s) 101, 102, 1375, 1502, 2347, 2787
PspOMI GGGCCC 1 cut(s) 99
PspPI GGNCC 7 cut(s) 99, 100, 961, 2485, 2611, 2746, 2786
PstI CTGCAG 2 cut(s) 1150, 2495
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 1 cut(s) 1939
PsyI GACNNNGTC 1 cut(s) 484
PvuI CGATCG 1 cut(s) 764
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 1 cut(s) 1176
RsaNI GTAC 1 cut(s) 1175
RseI CAYNNNNRTG 4 cut(s) 1022, 1130, 2055, 2142
SapI GCTCTTC 1 cut(s) 1115
SaqAI TTAA 9 cut(s) 15, 309, 506, 641, 726, 1058, 1982, 2304, 2321
Sau3AI GATC 8 cut(s) 133, 761, 772, 1684, 1735, 1741, 1795, 1939
Sau96I GGNCC 7 cut(s) 99, 100, 961, 2485, 2611, 2746, 2786
SchI GAGTC 6 cut(s) 47, 470, 697, 2093, 2480, 2675
ScrFI CCNGG 4 cut(s) 388, 1438, 1707, 1753
SduI GDGCHC 5 cut(s) 103, 424, 867, 1567, 2623
SfcI CTRYAG 4 cut(s) 1146, 2420, 2491, 2653
SinI GGWCC 3 cut(s) 961, 2485, 2746
SmiMI CAYNNNNRTG 4 cut(s) 1022, 1130, 2055, 2142
SmlI CTYRAG 1 cut(s) 1431
SmoI CTYRAG 1 cut(s) 1431
SnaBI TACGTA 1 cut(s) 1592
SphI GCATGC 1 cut(s) 1679
SseBI AGGCCT 1 cut(s) 1546
SsiI CCGC 6 cut(s) 673, 829, 1672, 1829, 2201, 2624
SspI AATATT 2 cut(s) 298, 1659
SspMI CTAG 8 cut(s) 248, 650, 849, 1542, 1856, 1964, 2526, 2585
StuI AGGCCT 1 cut(s) 1546
StyD4I CCNGG 4 cut(s) 386, 1436, 1705, 1751
StyI CCWWGG 2 cut(s) 1541, 1597
TaaI ACNGT 7 cut(s) 165, 704, 1822, 2092, 2376, 2508, 2735
TaiI ACGT 5 cut(s) 440, 1054, 1594, 2163, 2620
TaqI TCGA 4 cut(s) 1210, 1683, 1870, 2385
TaqII GACCGA 2 cut(s) 978, 2790
TatI WGTACW 1 cut(s) 1174
TauI GCSGC 2 cut(s) 675, 1675
TfiI GAWTC 8 cut(s) 78, 929, 1296, 1640, 2114, 2194, 2393, 2546
Tru1I TTAA 9 cut(s) 15, 309, 506, 641, 726, 1058, 1982, 2304, 2321
Tru9I TTAA 9 cut(s) 15, 309, 506, 641, 726, 1058, 1982, 2304, 2321
TscAI CASTG 8 cut(s) 127, 168, 709, 1042, 1650, 1825, 2379, 2576
TseFI GTSAC 8 cut(s) 140, 238, 319, 604, 880, 1601, 2350, 2762
Tsp45I GTSAC 8 cut(s) 140, 238, 319, 604, 880, 1601, 2350, 2762
TspGWI ACGGA 1 cut(s) 700
TspRI CASTG 8 cut(s) 127, 168, 709, 1042, 1650, 1825, 2379, 2576
Tth111I GACNNNGTC 1 cut(s) 484
VpaK11BI GGWCC 3 cut(s) 961, 2485, 2746
VspI ATTAAT 1 cut(s) 1058
XagI CCTNNNNNAGG 1 cut(s) 2432
XapI RAATTY 3 cut(s) 953, 1808, 2339
XceI RCATGY 8 cut(s) 649, 863, 1129, 1679, 1770, 2060, 2143, 2770
XcmI CCANNNNNNNNNTGG 3 cut(s) 922, 1889, 2144
XmaJI CCTAGG 1 cut(s) 1541
XmnI GAANNNNTTC 2 cut(s) 177, 998
XspI CTAG 8 cut(s) 248, 650, 849, 1542, 1856, 1964, 2526, 2585
ZraI GACGTC 1 cut(s) 2161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.