Rh1BG197300

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0007735
Physical Location & Seq
Forward (+)
31400542 .. 31410060
9519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG197300.1

Sequence Viewer

Length: 4350 bp
ATGATTTACACAAATTTCCGTCTTGTTACCCCGCTCCTCCTCCACTCCCCTTTCTGTTCAATTAGATTTGCACTTCATATTGTTGGATGCAGTGAAATGACTAATACGTTATGTACTTGTCTTCAGCATATTTCTGAATTTAGCTTGTTTTCTCCTACCCTGAGGTCTCTAAAGAATTTTTTTTTTCTTTTTTTTATATGGAACAGGTTTTTAGGTGCAATTAAGCAATACTTATGTCTGTCACTGTTAAAGAACAGCGCGTCAACTCTTATGATAGTTTTTCAGCTATCTTGTTCCATCTTCATTAGTCTGGTTTCAAGATTTAGAGCTGGATTGAAAGCAGAGATTGGAGTATTCTTTCCTATGATTGTTCTCAGAGTTTTAGAGAATGTTGCACAACCTAATTTTCAGCAGAAGATGATAGTGCTTCGGTTTCTTGAGAAGCTCTGTGTTGATTCCCAGATCTTGGTCGACATATTTATCAATTATGATTGCGATGTCAATTCATCAAACATATTTGAGAGGATGGTAAATGGCCTTCTTAAAACTGCCCAAGGTGTCCCTCCTGGTGTTGCCACCACGTTATTACCTCCTCAAGAGGCAAATATGAAACTTGAAGCCATGAGGTGTTTAGTTGCTATTCTGAGGTCAATCGGGGACTGGATGAACAAACAATTGCGCATTCCAGATCCTCATTCTACCAAGAAATCTGAAGTAAATGAGAATAGTTCTGAACCAGGAGCTCTTCCCATGGCAAATGGGAATGGTGAGGAGTCTGTTGAAGGATCAGATTCTCATTCTGAAGCCTCCAGTGAAGCCTCTGATGCTTTGACAATCGAGCAACGCCGAGCTTACAAGCTGGAACTTCAGGAAGGTATATCTCTTTTCAATCGGAAACCTAAGAAAGGAATTGAGTTTCTTATCAATGCAAATAAGGTGGGCAATTCACCCGAGGAGATAGCTGCTTTTCTAAAAAATGCATCTGGTTTGAACAAGACTTTGATTGGAGACTACCTTGGAGAAAGAGAAGAATTATCTCTAAAAGTAATGCATGCTTATGTGGATTCCTTTGATTTTCAAGACTTGGAGTTTGATGAGGCAATCAGGTCCTTTCTTCAAGGCTTTAGGTTGCCTGGTGAGGCACAGAAGATTGACAGAATAATGGAAAAGTTTGCTGAGCGCTATTGCAAGTGCAATCCAAAGGCTTTTACTAGTGCTGATACAGCCTATGTCCTTGCTTACTCCGTGATACTGCTCAATACTGATGCTCATAACCCGATGGTGAAGAGCAAGATGTCGGCTGATGATTTTATAAGAAACAATCGTGGCATAGATGATGGAAAAGATCTACCTGAGCAGTACTTGAGGTCATTGTATGAACGAATATCAAGAAAGGAGATAAAAATGAAAGAATATGATTTGGCTCCTCAGCAGATACAGTCGGTGAACTCTAACCGTCTTTTGGGCTTGGATAGTATCTTGAATATTGTGATCCGTAAACGGGGGGATAGTCAATTGGAAACCAGTGATGATCTTATCAAGCATATGCAAGAGCAATTCCAAGAAAAAGCTCGGAAATCTGAGTCAGTTTACTATGCTGCAACAGATGTGGTAATTCTTAGATTCATGGTTGAAGTCTGCTGGGCTCCTATGCTGGCTGCCTTCAGTGTTCCACTTGACCAAACTGATGATGAAGTAGTCATTTCTCTGTGTCTTGAAGGGATTCGTTATGCTATCCATGTAACTGCCGCAATGTCCATGAAGACCCATAGAGATGCTTTTGTGACTTCACTGGCGAAGTTTACTTCCCTTCACTCTCCTGCTGATATCAAGCAGAAAAACATAGAAGCAATCAAGGCAATAGTGACAATTGCAGATGAAGATGGGAACTACCTGCAAGAAGCTTGGGAGCATATTTTGACATGTGTTTCCCGCTTTGAACATCTTCATCTCCTGGGAGAGGGTGCTCCTCCAGATGCTACTTTCTTTGCTTTTCCTCAGAATGAATCAGAAAAATCAAAGCAAGCCAAGTCAACTGTCCTTCCTGTTTTAAAAAAGAAGGGCCAAGGGAAAATGCAGTATGCTGCTGCTGCCGTGTTGAGGGGTTCATATGATAGTGCTGGTATTGGTGGGAATTCTTCTGGGATGGTCACATCAGAACAGATGAACAATTTGGTTTCTAACTTAAATATGCTAGAACAAGTTGGAGACATGAGCCGCATATTCACACGGAGTCAAAAGCTGAACAGTGAGGCAATTATAGATTTTGTTAAGGCTCTTTGCAAGGTCTCTATGGAGGAATTGCGATCTGCATCTGATCCTCGGGTTTTCAGCCTTACGAAGATGGTTGAGATTGCGCATTATAACATGAACCGCATCAGGCTTGTGTGGTCTAGCATCTGGCATGTACTCTCTAATTTCTTCGTGACAATTGGCTGTTCTGAAAACCTGTCTATTGCAATTTTTGCTATGGATTCTCTACGCCAATTGTCAATGAAATTCTTGGAGCGAGAAGAGCTGGCTAATTATAATTTTCAGAATGAGTTTATGAAACCTTTTGTCATTGTTATGCGTAAAAGTAGTGCAGTTGAGATACGAGAATTAATCATCAGATGTGTCTCCCAAATGGTTTTATCTCGTGTGAATAGAGTCAAATCTGGATGGAAGAGCATGTTCATGGTCTTCACTACGGCAGCGTATGATGACCACAAAAATATTGTACTCTTGGCCTTTGAGATAATTGAGAAGATTATTCGCGACTACTTTCCGTACATCACTGAGACTGAAACCACTACTTTTACTGATTGCGTAAATTGTCTAATTGCATTCACCAATAGTAGATTTAACAAAGACATTAGCCTCAATGCTATTGCTTTTCTTCGGTTCTGTGCAACAAAACTGGCTGAGGGAGGTCTTAGTTCCTCATCAAGAAATAAGGACAAGGATGCATCTGGAAAAATATCTCCAACGTCACCTCAAGGATGGAAAGAAGGAAGACAAGATAATGGGGGAATGCCAGACAAGGATGACCATCTTTATTTCTGGTTCCCTTTATTGGCTGGTTTGTCCGAACTTAGCTTTGATCCTAGGCCTGAAATCAGGAAGAGTGCCTTACAAGTGCTGTTTGAAACTCTACGTAACCATGGTCACCTTTTCTCTCTACCTTTGTGGGAAAAGGTGTTTGAATCAGTGCTATTTCCAATATTTGACTATGTGCGCCATGCTATTGATCCTTCTGGGGAAAGTTCACCAGAGCAAGGGATTGACAGTGAAACAGGCGAGCTTGATCAAGATGCCTGGATGTACGAGACATGCACATTGGCACTCCAATTAGTTGTTGATCTTTTTGTCAAGTTTTATGACACTGTCAATCCTCTTTTGAAGAAAGTTTTGGATCTTCTAGTCAGTTTTATTGGACGACCTCACCAAAGCCTAGCTGGCATAGGTATTGCTGCATTTGTGCGATTGATGAGCAATGCAGGGGATCTCTTTTCTGATGAGAAGTGGCTTGAAGTGGTTTCATCATTAAAAGAAGCTGCCAATTCAACTCTTCCTGATTTTTCTTTCATTCTTAACGGAGATAGCATCATGGGAATCCTAGACTCTTCTTCTAGTAGAGAAGATCATGGAGGGTCTGGGAGGCCTGATGATGATTCAGAGAGACTAAGGACAAACCATCTTTATGCTGGCTTGGCTGATGTTAAGTGCCGAGCTGCTGTTCAGCTATTATTGATTCAGGCAGTGATGGAGATTTACACCATGTATCGTTCTCACCTTTCATCGAATAACACATTAATCCTCTTCAATGCTCTGCATGATTTGGCATCCCATGCTCACAAAGTTAACACTGATACCACTTTACGGGCAAGGCTACAAGAGTTTGGCTCCATGACGCAAATGCAAGACCCTCCACTGTTACGTATTGAGAACGAATCTTACCAAATCTGCCTTACATTCTTACAGAATCTTGTAGAGGACAGGCCTCCAAGTTATGATGAGGTGGAGGTAGAGTCCCACATTGTGGACCTTTGCAAGGAGGTTTTACAGTTCTATATTGAAGCCGCTAGCTCTGGGAAAATATCTGAATCATCTAATGGGCAGCAGCACCATTGGCTGATCCCTCTAGGTTCTGGGCGACGGAGAGAATTGGCTGCACGCGCACCGCTTATTGTGGCGACTCTCCAGGCCATCTGTTGCTTGGGAGAGACTTCATTTGAAAATAACTTGTCTCATTTCTTCCCGCTGCTTGCAACCTTGATAACTTGTGAACATGGGTCCGATGAGGTCCAGGCTGCCCTTAGTGACATGCTCAGCTCATCTGTGGGTCCTGTTTTGCTTCGATCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001817 GO:0001819 GO:0001881 GO:0003674 GO:0005085 GO:0005086 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005815 GO:0005829 GO:0005856 GO:0005874 GO:0005879 GO:0005881 GO:0005929 GO:0005930 GO:0006810 GO:0006887 GO:0006892 GO:0006893 GO:0006996 GO:0007032 GO:0007154 GO:0007165 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009553 GO:0009561 GO:0009987 GO:0010256 GO:0012505 GO:0015031 GO:0015630 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019899 GO:0023051 GO:0023052 GO:0030425 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032279 GO:0032280 GO:0032501 GO:0032502 GO:0032588 GO:0032680 GO:0032760 GO:0032838 GO:0032940 GO:0033036 GO:0034237 GO:0034613 GO:0035556 GO:0036477 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043112 GO:0043170 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044260 GO:0044309 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044441 GO:0044444 GO:0044446 GO:0044447 GO:0044456 GO:0044463 GO:0044464 GO:0045184 GO:0045202 GO:0046903 GO:0048193 GO:0048229 GO:0048518 GO:0048856 GO:0050789 GO:0050794 GO:0050811 GO:0050896 GO:0051018 GO:0051020 GO:0051179 GO:0051234 GO:0051239 GO:0051240 GO:0051641 GO:0051716 GO:0055037 GO:0061951 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072657 GO:0072659 GO:0090150 GO:0097014 GO:0097447 GO:0097458 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098794 GO:0098876 GO:0098984 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0120025 GO:0120038 GO:1903555 GO:1903557 GO:1990778
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1449

Amino Acids

162.92

Weight (kDa)

5.66

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 63 - 172 9.9e-31 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 286 - 467 2.9e-78 Sec7 domain
Sec7-like_HDS PF09324 833 - 915 1.3e-29 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1005 - 1093 2e-12 C-terminal region of Mon2 protein
BIG2_C PF20252 1239 - 1438 4.2e-43 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1313, 2364, 2529
Acc16I TGCGCA 2 cut(s) 680, 2358
Acc36I ACCTGC 1 cut(s) 1902
AccB7I CCANNNNNTGG 2 cut(s) 466, 4021
AccBSI CCGCTC 1 cut(s) 34
AccI GTMKAC 1 cut(s) 471
AccII CGCG 3 cut(s) 260, 2757, 4158
AciI CCGC 8 cut(s) 32, 1749, 1933, 2218, 2374, 4062, 4163, 4241
AclWI GGATC 9 cut(s) 683, 793, 1486, 2312, 3077, 3224, 3402, 3492, 4111
AcsI RAATTY 5 cut(s) 13, 137, 175, 2134, 2498
AcuI CTGAAG 5 cut(s) 107, 732, 822, 851, 1648
AdeI CACNNNGTG 1 cut(s) 4021
AfaI GTAC 6 cut(s) 115, 1361, 2409, 2721, 2771, 3305
AfeI AGCGCT 1 cut(s) 1181
AflIII ACRYGT 1 cut(s) 1922
AhlI ACTAGT 1 cut(s) 1211
AjnI CCWGG 7 cut(s) 565, 736, 1132, 1953, 3296, 4182, 4287
AjuI GAANNNNNNNTTGG 2 cut(s) 3374, 3406
Alw21I GWGCWC 2 cut(s) 745, 1969
AlwI GGATC 9 cut(s) 683, 793, 1486, 2312, 3077, 3224, 3402, 3492, 4111
Ama87I CYCGRG 2 cut(s) 950, 2322
Aor51HI AGCGCT 1 cut(s) 1181
AoxI GGCC 7 cut(s) 535, 2062, 2727, 3089, 3641, 3980, 4185
ApoI RAATTY 5 cut(s) 13, 137, 175, 2134, 2498
ArsI GACNNNNNNTTYG 2 cut(s) 1073, 1105
AseI ATTAAT 2 cut(s) 2603, 3794
Asp700I GAANNNNTTC 2 cut(s) 1722, 1944
AspA2I CCTAGG 1 cut(s) 3086
AspLEI GCGC 6 cut(s) 260, 681, 1182, 2359, 3219, 4160
AspS9I GGNCC 6 cut(s) 1107, 2062, 4024, 4275, 4285, 4325
AsuNHI GCTAGC 1 cut(s) 4064
AvaI CYCGRG 2 cut(s) 950, 2322
AvaII GGWCC 5 cut(s) 1107, 4024, 4275, 4285, 4325
AvrII CCTAGG 1 cut(s) 3086
AxyI CCTNAGG 1 cut(s) 161
BanII GRGCYC 2 cut(s) 745, 1648
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
BauI CACGAG 1 cut(s) 2637
BbsI GAAGAC 4 cut(s) 113, 1769, 2674, 3001
Bbv12I GWGCWC 2 cut(s) 745, 1969
BbvCI CCTCAGC 2 cut(s) 1428, 2904
BceAI ACGGC 2 cut(s) 2078, 2706
BciT130I CCWGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
BclI TGATCA 1 cut(s) 3286
BcuI ACTAGT 1 cut(s) 1211
BfoI RGCGCY 1 cut(s) 1183
BfuAI ACCTGC 1 cut(s) 1902
BglI GCCNNNNNGGC 1 cut(s) 3438
BglII AGATCT 2 cut(s) 462, 1345
BlnI CCTAGG 1 cut(s) 3086
BlpI GCTNAGC 2 cut(s) 1176, 4310
BmcAI AGTACT 1 cut(s) 1361
Bme1390I CCNGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
Bme18I GGWCC 5 cut(s) 1107, 4024, 4275, 4285, 4325
BmeT110I CYCGRG 2 cut(s) 950, 2322
BmgT120I GGNCC 6 cut(s) 1107, 2062, 4024, 4275, 4285, 4325
BmiI GGNNCC 6 cut(s) 1425, 1647, 3047, 3886, 4276, 4326
BmrFI CCNGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
BmtI GCTAGC 1 cut(s) 4068
BpiI GAAGAC 4 cut(s) 113, 1769, 2674, 3001
BplI GAGNNNNNCTC 2 cut(s) 3869, 3901
BpmI CTGGAG 3 cut(s) 793, 1956, 4166
Bpu10I CCTNAGC 3 cut(s) 1353, 1428, 2904
Bpu1102I GCTNAGC 2 cut(s) 1176, 4310
BpuEI CTTGAG 4 cut(s) 458, 579, 1384, 2961
BsaAI YACGTR 2 cut(s) 3137, 3920
BsaBI GATNNNNATC 2 cut(s) 2311, 3030
BsaI GGTCTC 2 cut(s) 171, 2293
BsaJI CCNNGG 9 cut(s) 553, 750, 951, 1015, 1954, 2065, 2321, 3086, 3142
BsaXI ACNNNNNCTCC 2 cut(s) 3995, 4025
Bse1I ACTGG 5 cut(s) 665, 810, 1524, 1797, 2904
Bse21I CCTNAGG 1 cut(s) 161
Bse3DI GCAATG 2 cut(s) 1758, 3481
Bse8I GATNNNNATC 2 cut(s) 2311, 3030
BseBI CCWGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
BseDI CCNNGG 9 cut(s) 553, 750, 951, 1015, 1954, 2065, 2321, 3086, 3142
BseJI GATNNNNATC 2 cut(s) 2311, 3030
BseMI GCAATG 2 cut(s) 1758, 3481
BseNI ACTGG 5 cut(s) 665, 810, 1524, 1797, 2904
BseRI GAGGAG 7 cut(s) 26, 29, 582, 785, 968, 1416, 1959
BseYI CCCAGC 1 cut(s) 1641
BsgI GTGCAG 2 cut(s) 2604, 4137
Bsh1236I CGCG 3 cut(s) 260, 2757, 4158
BshFI GGCC 7 cut(s) 537, 2064, 2729, 3091, 3643, 3982, 4187
BsiHKAI GWGCWC 2 cut(s) 745, 1969
BsiHKCI CYCGRG 2 cut(s) 950, 2322
BslFI GGGAC 3 cut(s) 545, 671, 3997
BsmFI GGGAC 3 cut(s) 545, 671, 3997
BsmI GAATGC 3 cut(s) 681, 2825, 3018
BsnI GGCC 7 cut(s) 537, 2064, 2729, 3091, 3643, 3982, 4187
Bso31I GGTCTC 2 cut(s) 171, 2293
BsoBI CYCGRG 2 cut(s) 950, 2322
Bsp1286I GDGCHC 3 cut(s) 745, 1648, 1969
Bsp1720I GCTNAGC 2 cut(s) 1176, 4310
Bsp19I CCATGG 2 cut(s) 750, 3142
Bsp68I TCGCGA 1 cut(s) 2757
BspACI CCGC 8 cut(s) 32, 1749, 1933, 2218, 2374, 4062, 4163, 4241
BspANI GGCC 7 cut(s) 537, 2064, 2729, 3091, 3643, 3982, 4187
BspFNI CGCG 3 cut(s) 260, 2757, 4158
BspLI GGNNCC 6 cut(s) 1425, 1647, 3047, 3886, 4276, 4326
BspMI ACCTGC 1 cut(s) 1902
BspOI GCTAGC 1 cut(s) 4068
BspPI GGATC 9 cut(s) 683, 793, 1486, 2312, 3077, 3224, 3402, 3492, 4111
BspQI GCTCTTC 4 cut(s) 750, 1280, 2508, 2660
BspTNI GGTCTC 2 cut(s) 171, 2293
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 2 cut(s) 1758, 3481
BsrI ACTGG 5 cut(s) 665, 810, 1524, 1797, 2904
BssECI CCNNGG 9 cut(s) 553, 750, 951, 1015, 1954, 2065, 2321, 3086, 3142
BssSI CACGAG 1 cut(s) 2637
BssT1I CCWWGG 6 cut(s) 553, 750, 1015, 2065, 3086, 3142
Bst2BI CACGAG 1 cut(s) 2637
Bst2UI CCWGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
Bst4CI ACNGT 9 cut(s) 246, 1440, 1457, 2038, 2249, 3269, 3367, 3915, 4047
Bst6I CTCTTC 8 cut(s) 750, 1280, 2508, 2660, 3098, 3555, 3610, 3806
BstAPI GCANNNNNTGC 2 cut(s) 2465, 3830
BstBAI YACGTR 2 cut(s) 3137, 3920
BstDSI CCRYGG 2 cut(s) 750, 3142
BstEII GGTNACC 1 cut(s) 3146
BstENI CCTNNNNNAGG 3 cut(s) 903, 1958, 4031
BstFNI CGCG 3 cut(s) 260, 2757, 4158
BstH2I RGCGCY 1 cut(s) 1183
BstHHI GCGC 6 cut(s) 260, 681, 1182, 2359, 3219, 4160
BstNI CCWGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
BstNSI RCATGY 6 cut(s) 1055, 1926, 2408, 2674, 3315, 4309
BstPI GGTNACC 1 cut(s) 3146
BstSCI CCNGG 7 cut(s) 565, 736, 1132, 1953, 3296, 4182, 4287
BstSNI TACGTA 2 cut(s) 3137, 3920
BstUI CGCG 3 cut(s) 260, 2757, 4158
BstV2I GAAGAC 4 cut(s) 113, 1769, 2674, 3001
BstX2I RGATCY 5 cut(s) 462, 688, 1345, 3394, 3484
BstYI RGATCY 5 cut(s) 462, 688, 1345, 3394, 3484
Bsu36I CCTNAGG 1 cut(s) 161
BsuRI GGCC 7 cut(s) 537, 2064, 2729, 3091, 3643, 3982, 4187
BtgI CCRYGG 2 cut(s) 750, 3142
BtgZI GCGATG 1 cut(s) 510
BtsI GCAGTG 2 cut(s) 97, 3747
BtuMI TCGCGA 1 cut(s) 2757
BveI ACCTGC 1 cut(s) 1902
CfoI GCGC 6 cut(s) 260, 681, 1182, 2359, 3219, 4160
Cfr13I GGNCC 6 cut(s) 1107, 2062, 4024, 4275, 4285, 4325
CseI GACGC 2 cut(s) 249, 3901
Csp6I GTAC 6 cut(s) 114, 1360, 2408, 2720, 2770, 3304
CspCI CAANNNNNGTGG 4 cut(s) 918, 953, 1590, 1625
CviQI GTAC 6 cut(s) 114, 1360, 2408, 2720, 2770, 3304
DraI TTTAAA 1 cut(s) 2052
DraIII CACNNNGTG 1 cut(s) 4021
Eam1104I CTCTTC 8 cut(s) 750, 1280, 2508, 2660, 3098, 3555, 3610, 3806
EarI CTCTTC 8 cut(s) 750, 1280, 2508, 2660, 3098, 3555, 3610, 3806
Ecl136II GAGCTC 1 cut(s) 743
Eco105I TACGTA 2 cut(s) 3137, 3920
Eco130I CCWWGG 6 cut(s) 553, 750, 1015, 2065, 3086, 3142
Eco147I AGGCCT 3 cut(s) 3091, 3643, 3982
Eco24I GRGCYC 2 cut(s) 745, 1648
Eco31I GGTCTC 2 cut(s) 171, 2293
Eco32I GATATC 1 cut(s) 1828
Eco47I GGWCC 5 cut(s) 1107, 4024, 4275, 4285, 4325
Eco47III AGCGCT 1 cut(s) 1181
Eco53kI GAGCTC 1 cut(s) 743
Eco57I CTGAAG 5 cut(s) 107, 732, 822, 851, 1648
Eco81I CCTNAGG 1 cut(s) 161
Eco88I CYCGRG 2 cut(s) 950, 2322
Eco91I GGTNACC 1 cut(s) 3146
EcoICRI GAGCTC 1 cut(s) 743
EcoNI CCTNNNNNAGG 3 cut(s) 903, 1958, 4031
EcoO109I RGGNCCY 2 cut(s) 1107, 4325
EcoO65I GGTNACC 1 cut(s) 3146
EcoRI GAATTC 1 cut(s) 2134
EcoRII CCWGG 7 cut(s) 565, 736, 1132, 1953, 3296, 4182, 4287
EcoRV GATATC 1 cut(s) 1828
EcoT14I CCWWGG 6 cut(s) 553, 750, 1015, 2065, 3086, 3142
EcoT22I ATGCAT 3 cut(s) 982, 1053, 2950
EcoT38I GRGCYC 2 cut(s) 745, 1648
ErhI CCWWGG 6 cut(s) 553, 750, 1015, 2065, 3086, 3142
FalI AAGNNNNNCTT 4 cut(s) 215, 247, 3095, 3127
FaqI GGGAC 3 cut(s) 545, 671, 3997
FauI CCCGC 3 cut(s) 39, 1940, 4248
FauNDI CATATG 2 cut(s) 1545, 2110
FbaI TGATCA 1 cut(s) 3286
FblI GTMKAC 1 cut(s) 471
FriOI GRGCYC 2 cut(s) 745, 1648
FspI TGCGCA 2 cut(s) 680, 2358
GlaI GCGC 6 cut(s) 259, 680, 1181, 2358, 3218, 4159
GsaI CCCAGC 1 cut(s) 1645
GsuI CTGGAG 3 cut(s) 793, 1956, 4166
HaeII RGCGCY 1 cut(s) 1183
HaeIII GGCC 7 cut(s) 537, 2064, 2729, 3091, 3643, 3982, 4187
HgaI GACGC 2 cut(s) 249, 3901
HhaI GCGC 6 cut(s) 260, 681, 1182, 2359, 3219, 4160
Hin6I GCGC 6 cut(s) 258, 679, 1180, 2357, 3217, 4158
HinP1I GCGC 6 cut(s) 258, 679, 1180, 2357, 3217, 4158
HincII GTYRAC 4 cut(s) 264, 472, 2034, 3844
HindII GTYRAC 4 cut(s) 264, 472, 2034, 3844
HindIII AAGCTT 1 cut(s) 1902
HpaI GTTAAC 1 cut(s) 3844
Hpy99I CGWCG 1 cut(s) 4140
HpyCH4III ACNGT 9 cut(s) 246, 1440, 1457, 2038, 2249, 3269, 3367, 3915, 4047
HpyCH4IV ACGT 5 cut(s) 107, 581, 2969, 3136, 3919
HpySE526I ACGT 5 cut(s) 107, 581, 2969, 3136, 3919
HspAI GCGC 6 cut(s) 258, 679, 1180, 2357, 3217, 4158
Ksp22I TGATCA 1 cut(s) 3286
KspAI GTTAAC 1 cut(s) 3844
LguI GCTCTTC 4 cut(s) 750, 1280, 2508, 2660
LmnI GCTCC 8 cut(s) 39, 740, 1429, 1651, 1909, 1972, 2506, 3890
MaeII ACGT 5 cut(s) 107, 581, 2969, 3136, 3919
MbiI CCGCTC 1 cut(s) 34
MfeI CAATTG 5 cut(s) 674, 1514, 1869, 2430, 2486
MflI RGATCY 5 cut(s) 462, 688, 1345, 3394, 3484
MhlI GDGCHC 3 cut(s) 745, 1648, 1969
MlyI GAGTC 7 cut(s) 782, 1592, 2242, 2658, 3596, 4019, 4171
MmeI TCCRAC 3 cut(s) 64, 2185, 2990
Mph1103I ATGCAT 3 cut(s) 982, 1053, 2950
MroXI GAANNNNTTC 2 cut(s) 1722, 1944
MslI CAYNNNNRTG 5 cut(s) 1056, 1773, 2567, 2675, 3681
MspA1I CMGCKG 1 cut(s) 4243
MspR9I CCNGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
MunI CAATTG 5 cut(s) 674, 1514, 1869, 2430, 2486
Mva1269I GAATGC 3 cut(s) 681, 2825, 3018
MvaI CCWGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
MvnI CGCG 3 cut(s) 260, 2757, 4158
NcoI CCATGG 2 cut(s) 750, 3142
NdeI CATATG 2 cut(s) 1545, 2110
NheI GCTAGC 1 cut(s) 4064
NlaIV GGNNCC 6 cut(s) 1425, 1647, 3047, 3886, 4276, 4326
NmeAIII GCCGAG 2 cut(s) 872, 3734
NmuCI GTSAC 8 cut(s) 240, 1783, 1864, 2149, 2425, 2970, 3146, 4301
NruI TCGCGA 1 cut(s) 2757
NsbI TGCGCA 2 cut(s) 680, 2358
NsiI ATGCAT 3 cut(s) 982, 1053, 2950
NspI RCATGY 6 cut(s) 1055, 1926, 2408, 2674, 3315, 4309
PaeI GCATGC 1 cut(s) 1055
PceI AGGCCT 3 cut(s) 3091, 3643, 3982
PciI ACATGT 1 cut(s) 1922
PciSI GCTCTTC 4 cut(s) 750, 1280, 2508, 2660
PctI GAATGC 3 cut(s) 681, 2825, 3018
PdmI GAANNNNTTC 2 cut(s) 1722, 1944
PflFI GACNNNGTC 1 cut(s) 3365
PflMI CCANNNNNTGG 2 cut(s) 466, 4021
PleI GAGTC 7 cut(s) 781, 1591, 2241, 2657, 3596, 4018, 4171
PpsI GAGTC 7 cut(s) 781, 1591, 2241, 2657, 3596, 4018, 4171
Ppu21I YACGTR 2 cut(s) 3137, 3920
PpuMI RGGWCCY 2 cut(s) 1107, 4325
PscI ACATGT 1 cut(s) 1922
PshBI ATTAAT 2 cut(s) 2603, 3794
PsiI TTATAA 3 cut(s) 1313, 2364, 2529
Psp124BI GAGCTC 1 cut(s) 745
Psp5II RGGWCCY 2 cut(s) 1107, 4325
Psp6I CCWGG 7 cut(s) 565, 736, 1132, 1953, 3296, 4182, 4287
PspEI GGTNACC 1 cut(s) 3146
PspFI CCCAGC 1 cut(s) 1641
PspGI CCWGG 7 cut(s) 565, 736, 1132, 1953, 3296, 4182, 4287
PspN4I GGNNCC 6 cut(s) 1425, 1647, 3047, 3886, 4276, 4326
PspPI GGNCC 6 cut(s) 1107, 2062, 4024, 4275, 4285, 4325
PspPPI RGGWCCY 2 cut(s) 1107, 4325
PsrI GAACNNNNNNTAC 2 cut(s) 3920, 3952
PsuI RGATCY 5 cut(s) 462, 688, 1345, 3394, 3484
PsyI GACNNNGTC 1 cut(s) 3365
RruI TCGCGA 1 cut(s) 2757
RsaI GTAC 6 cut(s) 115, 1361, 2409, 2721, 2771, 3305
RsaNI GTAC 6 cut(s) 114, 1360, 2408, 2720, 2770, 3304
RseI CAYNNNNRTG 5 cut(s) 1056, 1773, 2567, 2675, 3681
SacI GAGCTC 1 cut(s) 745
SalI GTCGAC 1 cut(s) 470
SapI GCTCTTC 4 cut(s) 750, 1280, 2508, 2660
Sau96I GGNCC 6 cut(s) 1107, 2062, 4024, 4275, 4285, 4325
ScaI AGTACT 1 cut(s) 1361
SchI GAGTC 7 cut(s) 782, 1592, 2242, 2658, 3596, 4019, 4171
ScrFI CCNGG 7 cut(s) 567, 738, 1134, 1955, 3298, 4184, 4289
SduI GDGCHC 3 cut(s) 745, 1648, 1969
SinI GGWCC 5 cut(s) 1107, 4024, 4275, 4285, 4325
SmiMI CAYNNNNRTG 5 cut(s) 1056, 1773, 2567, 2675, 3681
SmlI CTYRAG 4 cut(s) 437, 594, 1363, 2976
SmoI CTYRAG 4 cut(s) 437, 594, 1363, 2976
SnaBI TACGTA 2 cut(s) 3137, 3920
SpeI ACTAGT 1 cut(s) 1211
SphI GCATGC 1 cut(s) 1055
SseBI AGGCCT 3 cut(s) 3091, 3643, 3982
SsiI CCGC 8 cut(s) 32, 1749, 1933, 2218, 2374, 4062, 4163, 4241
SspI AATATT 3 cut(s) 1486, 2716, 3204
SstI GAGCTC 1 cut(s) 745
StuI AGGCCT 3 cut(s) 3091, 3643, 3982
StyD4I CCNGG 7 cut(s) 565, 736, 1132, 1953, 3296, 4182, 4287
StyI CCWWGG 6 cut(s) 553, 750, 1015, 2065, 3086, 3142
TaaI ACNGT 9 cut(s) 246, 1440, 1457, 2038, 2249, 3269, 3367, 3915, 4047
TaiI ACGT 5 cut(s) 110, 584, 2972, 3139, 3922
TaqI TCGA 4 cut(s) 471, 837, 3782, 4339
TatI WGTACW 4 cut(s) 113, 1359, 2407, 2719
TauI GCSGC 3 cut(s) 1751, 2220, 4064
TseFI GTSAC 8 cut(s) 240, 1783, 1864, 2149, 2425, 2970, 3146, 4301
Tsp45I GTSAC 8 cut(s) 240, 1783, 1864, 2149, 2425, 2970, 3146, 4301
TspGWI ACGGA 7 cut(s) 8, 1234, 1484, 2245, 2757, 3591, 4153
Tth111I GACNNNGTC 1 cut(s) 3365
Van91I CCANNNNNTGG 2 cut(s) 466, 4021
VpaK11BI GGWCC 5 cut(s) 1107, 4024, 4275, 4285, 4325
VspI ATTAAT 2 cut(s) 2603, 3794
XagI CCTNNNNNAGG 3 cut(s) 903, 1958, 4031
XapI RAATTY 5 cut(s) 13, 137, 175, 2134, 2498
XceI RCATGY 6 cut(s) 1055, 1926, 2408, 2674, 3315, 4309
XcmI CCANNNNNNNNNTGG 3 cut(s) 3434, 3683, 4195
XmaJI CCTAGG 1 cut(s) 3086
XmiI GTMKAC 1 cut(s) 471
XmnI GAANNNNTTC 2 cut(s) 1722, 1944
ZrmI AGTACT 1 cut(s) 1361
Zsp2I ATGCAT 3 cut(s) 982, 1053, 2950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.