Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Reverse (-)

1252005 .. 1257893

Gene Structure 5889 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G04580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4014 bp

ATGGCGGGTGACGATTTGGTGTTTGCTGTTAATGGAGAGAAATTTGAAGTCTTGTCAGTTAATCCTTCTACGACTTTGCTCGAGTTCTTGCGTTCCAATACTTGCTTTAAGAGTGTCAAGCTCAGCTGTGGCGAAGGGGGTTGTGGTGCTTGCATCGTAATTCTATCAAAATACGATCCTGTGTTAGACCAAGTCGAGGAGTATTCTATAAACTCTTGCCTGACCCTTCTTTGCAGCTTAAATGGGTGCTCCATAACTACATCCGATGGACTCGGAAACACTGAAAAAGGGTTCCACCCAATTCACAAGAGATTTGCGGGGTTCCATGCCTCTCAATGTGGTTTCTGTACCCCAGGGATGTGCATCTCTCTCTACTCCGCTCTTTCAAAAGCCCATAACTCACAATCTTCTCCAGACTACCTGACTGCTCTTGCAGCAGAGAAGTCCATTGCTGGAAATCTTTGTCGCTGCACCGGGTATCGTCCCATTGCTGATGCTTGTAAGAGTTTTGCTTCCGATGTCGATATAGAGGATTTGGGCTTCAACTCTTTTTGGAGGAAAGGAGAAAGCAGAGAAGAGATGTTAAAGAAATTGCCTCCTTACAACCCTGAGAAAGATCTTATCACTTTTCCGGATTTCTTGAAGGAAAAGATTAAGTGTCAACACAATGTTTTGGATCAGACGAGGTATCATTGGAGCACTCCTGGTAGCGTCGCAGAGCTTCAGGAAATATTGGCAACAACCAACCCTGGCAAAGATAGAGGTTTGATAAAATTAGTTGTTGGCAACACGGGAACAGGTTACTACAAAGAAGAGAAACAATATGGTAGATATATTGATATTAGCCACATTCCAGAGATGTCAATGATCAAGAAAGATGACAGAGAAATTGAAATTGGAGCAGTTGTTACCATATCTAAAGTTATTGATGCTTTGATGGAGGAAAATACATCTGCTTATGTTTTCAAGAAAATCGGTGTCCATATGGAGAAGGTGGCTAACCATTTTATCAGAAACTCAGGGAGTATCGGTGGTAATCTAGTGATGGCACAGAGCAAAAGTTTTCCTTCTGACATAACCACACTTTTGCTCGCTGCAGATGCATCTGTGCATATGATAAATGCTGGGAGACACGAGAAGCTTAGGATGGGAGAGTATCTTGTGTCGCCTCCAATATTAGACACTAAAACTGTTCTTTTAAAGGTTCATATTCCTAGGTGGATTGCTTCTTCCACTACTGGTTTGCTTTTCGAAACCTATCGGGCTGCACTTCGCCCTATTGGAAGTGCATTGCCTTATATCAACGCTGCTTTCTTAGCAGTAGTCTCTCACGATGCATCATCAAGCGGCATCATTGTGGATAAATGCCGGTTGGCATTTGGTTCTTATGGTGGGTATCATTCCATCAGGGCGAGGGAAGTTGAGGATTTCTTGACGGGTAAAATACTCAGCCACAGTGTTTTATATGAAGCTGTGAGGTTACTTAAAGGGATCATAGTTCCCAGCATAGATACCTCATATTCTGAGTATAAGAAAAGCTTGGCCGTTGGGTTTCTCTTTGATTTTCTCTATCCTCTGATCGAGAGTGGCAGTTGGGATTCCGAAGGAAAGCATATCGATGGGCACATCGATCCAACAATATGTCTACCTTTGCTGTCATCTGCGCAGCAGGTGTTTGAAAGTAAAGAATATCACCCTGTTGGTGAAGCCATTATCAAATTTGGAGCTGAAATGCAGGCTTCTGGTGAAGCTGTTTACGTTGATGATATTCCATCCTTACCCCATTGTCTACATGGAGCATTCATTTATAGCACAAAGCCATTGGCTTGGATAAAGAGCGTTGGTTTCTCAGGAAATGTGACACCAATTGGAGTTTTGGCAGTTATTACTTTCAAAGATATACCTGAAGTGGGGCAAAACATTGGTTACATAACGATGTTTGGCACAGGGCTTCTATTTGCAGATGAAGTTACTATATCTGCCGGACAAATAATTGCTCTTGTGGTTGCAGACACACAAAAGCATGCAGATATGGCAGCACACCTTGCAGTAGTTGAGTACGATTCAAGGAATATAGGAACACCAGTATTATCTGTGGAGGATGCAGTCAAAAGGTCCAGCCTTTTTGAGGTTCCTCCCGAGTATCAGCCCGAACCTGTTGGCGATATTTCAAAAGGAATGGCTGAAGCTGATCGCAAGATACGCTCAGTAGAGTTGAGACTTGGGTCACAGTACTTCTTCTATATGGAGACACAAACAGCACTTGCCTTGCCAGATGAAGACAACTGTTTAGTGGTTTACAGTTCAACTCAAGCACCCGAGTTCACACAAACAGTAATTGCTACATGTCTTGGAATCCCGGAGCATAACGTTCGAGTCATCACCAGAAGAGTTGGAGGTGGCTTTGGTGGAAAAGCTATAAAGTCTATGCCTGTTGCGACAGCATGTGCGCTTGCTGCAAAAAAAATGCAGCGTCCTGTAAGGATATACGTCAACCGTAAGACTGATATGATAATGGCTGGAGGAAGGCATCCCCTGAAAATAACTTACAGTGTAGGATTCAGATCTGACGGTAAACTCACAGCACTAGACCTAAATTTGTTTATAGATGCAGGGTCAGATGTGGATGTAAGTCTAGTGATGCCACAGAATATAATGAACTCCCTGAGGAAATATGACTGGGGTGCATTGTCATTTGACATCAAAGTGTGTAAGACAAACCTTCCGAGCAGAACGTCTTTGAGGGCACCCGGTGAAGTACAAGGTTCATACATTGCTGAATCCATTATTGAGAACGTAGCATCTTCCCTGAAGATGGACGTGGATGTTGTGAGAAGGATAAATCTTCATACGTACGAAAGCCTCCGCAAGTTTTATAAGCAAGCTGCTGGTGAGCCTGATGAATATACTTTGCCTTTATTGTGGGACAAATTGGAGGTATCTGCAGATTTCAGGCGAAGGGCTGAATCTGTGAAAGAGTTTAACCGATGTAACATATGGCGCAAAAGGGGGATTTCTCGAGTACCAATCATCCATCTAGTTATACATAGGCCAACTCCAGGAAAAGTAAGCATTTTGAATGATGGTTCAGTTGCGGTTGAGGTTGCAGGGATTGAGGTGGGACAAGGGTTATGGACAAAGGTACAACAGATGGTTGCTTATGGTCTTGGTATGATAAAATGCGAGGGAAGTGATGATCTGCTTGAAAGAATACGGCTTCTTCAGACTGACACGCTCAGTATGTCTCAAAGCAGTTACACTGCGGGTAGCACAACATCAGAGAACTGTTGTGAAGCAGTGAGGCTTTGCTGCGGTATCTTGGTGGAGAGGCTGAGACCTACCATGAATCAGATTCTGGAGAATGCTCGATCCGTGACATGGGACATGCTCATTCAACAGGCGAATGCTCAATCTGTGGACCTATCAGCTCGAACCTTTTATAAGCCAGAGTCCTCTTCTGCAGAATATCTCAACTATGGAGTTGGAGCGAGTGAGGTGGAGGTTGACCTTGTGACAGGAAGAACAGAAATTATAAGATCAGATATTATCTATGACTGTGGAAAAAGCCTTAATCCTGCTGTTGATTTAGGACAGATTGAAGGAGCGTTTGTTCAAGGAATCGGATTTTTCATGTATGAGGAGTACACGACAAATGAAAACGGGCTTGTGAATGAAGAAGGTACATGGGACTACAAAATACCTACAATAGACACAATTCCCAAACAATTCAACGTTCAGATTCTCAACAGTGGTCATCACAAGAACCGCGTTCTCTCCTCTAAAGCTTCAGGTGAGCCTCCGTTGCTTGTGGCGGCTTCAGTGCACTGTGCAACAAGATCAGCCATTAGAGAAGCTCGAAAACAGTACCTATCATGGAATTGCATTGATGATGATCATAGAGAAAGATGTGATCTTGGTTTTGAGTTACCGGTTCCAGCGACTATGCCGGTGGTGAAGCAGCTTTGTGGCCTTGAAAGCATAGAGAAGTACTTAGAATGGAAAACATATCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1337

Amino Acids

147.3

Weight (kDa)

5.93

Isoelectric Point (pI)

38.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	9 - 77	6.8e-08	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	87 - 168	1.2e-21	[2Fe-2S] binding domain
FAD_binding_5	PF00941	229 - 407	6.5e-42	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	419 - 524	6.9e-15	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	581 - 690	1.9e-32	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	720 - 949	7.8e-80	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	979 - 1240	1.2e-71	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	2877, 3441, 3533
AarI	CACCTGC	1 cut(s)	1660
Acc16I	TGCGCA	1 cut(s)	1665
Acc36I	ACCTGC	1 cut(s)	1660
AccB1I	GGYRCC	1 cut(s)	2746
AccBSI	CCGCTC	1 cut(s)	380
AccI	GTMKAC	2 cut(s)	1645, 1789
AccII	CGCG	1 cut(s)	3768
AccIII	TCCGGA	1 cut(s)	631
AclI	AACGTT	2 cut(s)	2370, 3732
AclWI	GGATC	5 cut(s)	170, 684, 1499, 1625, 3363
AcoI	YGGCCR	1 cut(s)	1542
AcsI	RAATTY	3 cut(s)	41, 1718, 2596
AcuI	CTGAAG	7 cut(s)	707, 1926, 2205, 2831, 3206, 3771, 3801
AdeI	CACNNNGTG	1 cut(s)	2753
AfiI	CCNNNNNNNGG	6 cut(s)	196, 1910, 2128, 2815, 3059, 3378
AflIII	ACRYGT	1 cut(s)	2345
AgeI	ACCGGT	1 cut(s)	3928
AjiI	CACGTC	1 cut(s)	2821
AjnI	CCWGG	4 cut(s)	352, 703, 748, 3058
AjuI	GAANNNNNNNTTGG	2 cut(s)	879, 911
AloI	GAACNNNNNNTCC	2 cut(s)	3594, 3626
Alw21I	GWGCWC	3 cut(s)	251, 701, 3825
Alw26I	GTCTC	6 cut(s)	1123, 1330, 2212, 2243, 3249, 3328
Alw44I	GTGCAC	1 cut(s)	3821
AlwI	GGATC	5 cut(s)	170, 684, 1499, 1625, 3363
AlwNI	CAGNNNCTG	1 cut(s)	3355
Ama87I	CYCGRG	4 cut(s)	80, 2138, 2318, 3018
Aor13HI	TCCGGA	1 cut(s)	631
AoxI	GGCC	3 cut(s)	1542, 3050, 3967
ApaLI	GTGCAC	1 cut(s)	3821
ApoI	RAATTY	3 cut(s)	41, 1718, 2596
AsiGI	ACCGGT	1 cut(s)	3928
AspA2I	CCTAGG	1 cut(s)	1214
AspLEI	GCGC	3 cut(s)	1666, 2452, 3003
AspS9I	GGNCC	2 cut(s)	2115, 3418
AsuC2I	CCSGG	3 cut(s)	475, 2360, 2751
AsuHPI	GGTGA	9 cut(s)	20, 1685, 1715, 1757, 2374, 2765, 2903, 3803, 3964
AsuII	TTCGAA	1 cut(s)	1251
AvaI	CYCGRG	4 cut(s)	80, 2138, 2318, 3018
AvaII	GGWCC	2 cut(s)	2115, 3418
AvrII	CCTAGG	1 cut(s)	1214
AxyI	CCTNAGG	1 cut(s)	2666
BaeGI	GKGCMC	3 cut(s)	1626, 2749, 3825
BanI	GGYRCC	1 cut(s)	2746
BauI	CACGAG	1 cut(s)	1133
BbsI	GAAGAC	1 cut(s)	2286
Bbv12I	GWGCWC	3 cut(s)	251, 701, 3825
BceAI	ACGGC	2 cut(s)	1529, 3230
BcgI	CGANNNNNNTGC	2 cut(s)	2354, 2388
BciT130I	CCWGG	4 cut(s)	354, 705, 750, 3060
BclI	TGATCA	2 cut(s)	867, 3892
BcnI	CCSGG	3 cut(s)	475, 2360, 2751
BcoDI	GTCTC	6 cut(s)	1123, 1330, 2212, 2243, 3249, 3328
BfaI	CTAG	5 cut(s)	1040, 1215, 2588, 2636, 3038
BfmI	CTRYAG	3 cut(s)	1095, 2943, 3459
BfuAI	ACCTGC	1 cut(s)	1660
BglII	AGATCT	2 cut(s)	616, 2564
BlnI	CCTAGG	1 cut(s)	1214
BlpI	GCTNAGC	1 cut(s)	122
BmcAI	AGTACT	2 cut(s)	2234, 3989
Bme1390I	CCNGG	7 cut(s)	354, 475, 705, 750, 2360, 2751, 3060
Bme18I	GGWCC	2 cut(s)	2115, 3418
BmeT110I	CYCGRG	4 cut(s)	80, 2138, 2318, 3018
BmgBI	CACGTC	1 cut(s)	2821
BmgT120I	GGNCC	2 cut(s)	2115, 3418
BmiI	GGNNCC	5 cut(s)	293, 323, 2133, 2748, 3933
BmrFI	CCNGG	7 cut(s)	354, 475, 705, 750, 2360, 2751, 3060
BmrI	ACTGGG	1 cut(s)	2689
BmuI	ACTGGG	1 cut(s)	2689
BoxI	GACNNNNGTC	1 cut(s)	2224
BpiI	GAAGAC	1 cut(s)	2286
BpmI	CTGGAG	4 cut(s)	396, 2541, 3042, 3377
Bpu10I	CCTNAGC	1 cut(s)	1142
Bpu1102I	GCTNAGC	1 cut(s)	122
Bpu14I	TTCGAA	1 cut(s)	1251
BpuEI	CTTGAG	1 cut(s)	2295
BpuMI	CCSGG	3 cut(s)	475, 2360, 2751
Bsa29I	ATCGAT	2 cut(s)	1617, 1629
BsaAI	YACGTR	1 cut(s)	2853
BsaBI	GATNNNNATC	3 cut(s)	362, 2563, 3891
BsaI	GGTCTC	1 cut(s)	3328
BsaJI	CCNNGG	4 cut(s)	352, 353, 748, 1214
BsaWI	WCCGGW	2 cut(s)	631, 3928
BsaXI	ACNNNNNCTCC	6 cut(s)	891, 921, 1015, 1045, 3594, 3624
Bsc4I	CCNNNNNNNGG	6 cut(s)	196, 1910, 2128, 2815, 3059, 3378
Bse118I	RCCGGY	3 cut(s)	1368, 3928, 3946
Bse1I	ACTGG	3 cut(s)	1243, 2084, 2684
Bse21I	CCTNAGG	1 cut(s)	2666
Bse3DI	GCAATG	4 cut(s)	447, 486, 1289, 2772
Bse8I	GATNNNNATC	3 cut(s)	362, 2563, 3891
BseAI	TCCGGA	1 cut(s)	631
BseBI	CCWGG	4 cut(s)	354, 705, 750, 3060
BseCI	ATCGAT	2 cut(s)	1617, 1629
BseDI	CCNNGG	4 cut(s)	352, 353, 748, 1214
BseGI	GGATG	9 cut(s)	260, 363, 1152, 1772, 2107, 2530, 2632, 2830, 3030
BseJI	GATNNNNATC	3 cut(s)	362, 2563, 3891
BseLI	CCNNNNNNNGG	6 cut(s)	196, 1910, 2128, 2815, 3059, 3378
BseMI	GCAATG	4 cut(s)	447, 486, 1289, 2772
BseNI	ACTGG	3 cut(s)	1243, 2084, 2684
BseRI	GAGGAG	3 cut(s)	212, 3653, 3766
BseSI	GKGCMC	3 cut(s)	1626, 2749, 3825
BseYI	CCCAGC	2 cut(s)	1124, 1502
BsgI	GTGCAG	2 cut(s)	454, 1251
Bsh1236I	CGCG	1 cut(s)	3768
BshFI	GGCC	3 cut(s)	1544, 3052, 3969
BshNI	GGYRCC	1 cut(s)	2746
BshTI	ACCGGT	1 cut(s)	3928
BshVI	ATCGAT	2 cut(s)	1617, 1629
BsiHKAI	GWGCWC	3 cut(s)	251, 701, 3825
BsiHKCI	CYCGRG	4 cut(s)	80, 2138, 2318, 3018
BsiSI	CCGG	8 cut(s)	474, 632, 1369, 1983, 2360, 2751, 3929, 3947
BsiWI	CGTACG	1 cut(s)	2853
BslFI	GGGAC	5 cut(s)	468, 2939, 3135, 3395, 3701
BslI	CCNNNNNNNGG	6 cut(s)	196, 1910, 2128, 2815, 3059, 3378
BsmAI	GTCTC	6 cut(s)	1123, 1330, 2212, 2243, 3249, 3328
BsmFI	GGGAC	5 cut(s)	468, 2939, 3135, 3395, 3701
BsmI	GAATGC	3 cut(s)	1799, 3367, 3409
BsnI	GGCC	3 cut(s)	1544, 3052, 3969
Bso31I	GGTCTC	1 cut(s)	3328
BsoBI	CYCGRG	4 cut(s)	80, 2138, 2318, 3018
Bsp119I	TTCGAA	1 cut(s)	1251
Bsp1286I	GDGCHC	5 cut(s)	251, 701, 1626, 2749, 3825
Bsp13I	TCCGGA	1 cut(s)	631
Bsp1720I	GCTNAGC	1 cut(s)	122
BspANI	GGCC	3 cut(s)	1544, 3052, 3969
BspDI	ATCGAT	2 cut(s)	1617, 1629
BspEI	TCCGGA	1 cut(s)	631
BspFNI	CGCG	1 cut(s)	3768
BspLI	GGNNCC	5 cut(s)	293, 323, 2133, 2748, 3933
BspMAI	CTGCAG	3 cut(s)	1099, 2947, 3463
BspMI	ACCTGC	1 cut(s)	1660
BspPI	GGATC	5 cut(s)	170, 684, 1499, 1625, 3363
BspT104I	TTCGAA	1 cut(s)	1251
BspT107I	GGYRCC	1 cut(s)	2746
BspTNI	GGTCTC	1 cut(s)	3328
BsrBI	CCGCTC	1 cut(s)	380
BsrDI	GCAATG	4 cut(s)	447, 486, 1289, 2772
BsrFI	RCCGGY	3 cut(s)	1368, 3928, 3946
BsrI	ACTGG	3 cut(s)	1243, 2084, 2684
BssAI	RCCGGY	3 cut(s)	1368, 3928, 3946
BssECI	CCNNGG	4 cut(s)	352, 353, 748, 1214
BssSI	CACGAG	1 cut(s)	1133
BssT1I	CCWWGG	1 cut(s)	1214
Bst2BI	CACGAG	1 cut(s)	1133
Bst2UI	CCWGG	4 cut(s)	354, 705, 750, 3060
Bst6I	CTCTTC	4 cut(s)	570, 807, 2383, 3460
BstAPI	GCANNNNNTGC	1 cut(s)	2045
BstBAI	YACGTR	1 cut(s)	2853
BstBI	TTCGAA	1 cut(s)	1251
BstC8I	GCNNGC	6 cut(s)	151, 1092, 1737, 2025, 2454, 2883
BstENI	CCTNNNNNAGG	1 cut(s)	2126
BstF5I	GGATG	9 cut(s)	260, 363, 1152, 1772, 2107, 2530, 2632, 2830, 3030
BstFNI	CGCG	1 cut(s)	3768
BstHHI	GCGC	3 cut(s)	1666, 2452, 3003
BstMAI	GTCTC	6 cut(s)	1123, 1330, 2212, 2243, 3249, 3328
BstMWI	GCNNNNNNNGC	9 cut(s)	434, 1100, 1316, 2033, 2045, 2202, 2456, 3802, 3975
BstNI	CCWGG	4 cut(s)	354, 705, 750, 3060
BstNSI	RCATGY	4 cut(s)	2027, 2349, 2448, 3388
BstPAI	GACNNNNGTC	1 cut(s)	2224
BstSCI	CCNGG	7 cut(s)	352, 473, 703, 748, 2358, 2749, 3058
BstSFI	CTRYAG	3 cut(s)	1095, 2943, 3459
BstSLI	GKGCMC	3 cut(s)	1626, 2749, 3825
BstSNI	TACGTA	1 cut(s)	2853
BstUI	CGCG	1 cut(s)	3768
BstV2I	GAAGAC	1 cut(s)	2286
BstX2I	RGATCY	2 cut(s)	616, 2564
BstXI	CCANNNNNNTGG	1 cut(s)	1827
BstYI	RGATCY	2 cut(s)	616, 2564
Bsu15I	ATCGAT	2 cut(s)	1617, 1629
Bsu36I	CCTNAGG	1 cut(s)	2666
BsuRI	GGCC	3 cut(s)	1544, 3052, 3969
BsuTUI	ATCGAT	2 cut(s)	1617, 1629
BtrI	CACGTC	1 cut(s)	2821
BtsCI	GGATG	9 cut(s)	260, 363, 1152, 1772, 2107, 2530, 2632, 2830, 3030
BtsI	GCAGTG	2 cut(s)	3258, 3303
BtsIMutI	CAGTG	8 cut(s)	279, 1462, 2557, 3258, 3303, 3754, 3823, 3825
BveI	ACCTGC	1 cut(s)	1660
Cac8I	GCNNGC	6 cut(s)	151, 1092, 1737, 2025, 2454, 2883
CaiI	CAGNNNCTG	1 cut(s)	3355
CfoI	GCGC	3 cut(s)	1666, 2452, 3003
Cfr10I	RCCGGY	3 cut(s)	1368, 3928, 3946
Cfr13I	GGNCC	2 cut(s)	2115, 3418
ClaI	ATCGAT	2 cut(s)	1617, 1629
CseI	GACGC	2 cut(s)	700, 2462
CspAI	ACCGGT	1 cut(s)	3928
DraI	TTTAAA	1 cut(s)	1200
DraIII	CACNNNGTG	1 cut(s)	2753
EaeI	YGGCCR	1 cut(s)	1542
Eam1104I	CTCTTC	4 cut(s)	570, 807, 2383, 3460
EarI	CTCTTC	4 cut(s)	570, 807, 2383, 3460
Eco105I	TACGTA	1 cut(s)	2853
Eco130I	CCWWGG	1 cut(s)	1214
Eco31I	GGTCTC	1 cut(s)	3328
Eco47I	GGWCC	2 cut(s)	2115, 3418
Eco57I	CTGAAG	7 cut(s)	707, 1926, 2205, 2831, 3206, 3771, 3801
Eco81I	CCTNAGG	1 cut(s)	2666
Eco88I	CYCGRG	4 cut(s)	80, 2138, 2318, 3018
EcoNI	CCTNNNNNAGG	1 cut(s)	2126
EcoRII	CCWGG	4 cut(s)	352, 703, 748, 3058
EcoT14I	CCWWGG	1 cut(s)	1214
EcoT22I	ATGCAT	2 cut(s)	1105, 1339
ErhI	CCWWGG	1 cut(s)	1214
FalI	AAGNNNNNCTT	4 cut(s)	1051, 1083, 1523, 1555
FaqI	GGGAC	5 cut(s)	468, 2939, 3135, 3395, 3701
FauI	CCCGC	2 cut(s)	310, 3256
FauNDI	CATATG	3 cut(s)	984, 1113, 2996
FbaI	TGATCA	2 cut(s)	867, 3892
FblI	GTMKAC	2 cut(s)	1645, 1789
FokI	GGATG	9 cut(s)	247, 370, 1159, 1759, 2114, 2517, 2639, 2837, 3017
FspBI	CTAG	5 cut(s)	1040, 1215, 2588, 2636, 3038
FspI	TGCGCA	1 cut(s)	1665
GlaI	GCGC	3 cut(s)	1665, 2451, 3002
GsaI	CCCAGC	2 cut(s)	1128, 1506
GsuI	CTGGAG	4 cut(s)	396, 2541, 3042, 3377
HaeIII	GGCC	3 cut(s)	1544, 3052, 3969
HapII	CCGG	8 cut(s)	474, 632, 1369, 1983, 2360, 2751, 3929, 3947
HgaI	GACGC	2 cut(s)	700, 2462
HhaI	GCGC	3 cut(s)	1666, 2452, 3003
Hin6I	GCGC	3 cut(s)	1664, 2450, 3001
HinP1I	GCGC	3 cut(s)	1664, 2450, 3001
HincII	GTYRAC	3 cut(s)	662, 2494, 3505
HindII	GTYRAC	3 cut(s)	662, 2494, 3505
HindIII	AAGCTT	3 cut(s)	1139, 1537, 3783
HpaII	CCGG	8 cut(s)	474, 632, 1369, 1983, 2360, 2751, 3929, 3947
HphI	GGTGA	9 cut(s)	20, 1685, 1715, 1757, 2374, 2765, 2903, 3803, 3964
Hpy99I	CGWCG	1 cut(s)	716
HpyCH4IV	ACGT	8 cut(s)	1758, 2370, 2490, 2735, 2796, 2820, 2852, 3732
HpyF10VI	GCNNNNNNNGC	9 cut(s)	434, 1100, 1316, 2033, 2045, 2202, 2456, 3802, 3975
HpySE526I	ACGT	8 cut(s)	1758, 2370, 2490, 2735, 2796, 2820, 2852, 3732
HspAI	GCGC	3 cut(s)	1664, 2450, 3001
Kpn2I	TCCGGA	1 cut(s)	631
Ksp22I	TGATCA	2 cut(s)	867, 3892
LmnI	GCTCC	8 cut(s)	254, 696, 899, 1724, 1796, 2362, 3485, 3602
MaeI	CTAG	5 cut(s)	1040, 1215, 2588, 2636, 3038
MaeII	ACGT	8 cut(s)	1758, 2370, 2490, 2735, 2796, 2820, 2852, 3732
MbiI	CCGCTC	1 cut(s)	380
MfeI	CAATTG	1 cut(s)	1866
MflI	RGATCY	2 cut(s)	616, 2564
MhlI	GDGCHC	5 cut(s)	251, 701, 1626, 2749, 3825
MlyI	GAGTC	3 cut(s)	264, 2385, 3458
MmeI	TCCRAC	3 cut(s)	1658, 2374, 3463
Mph1103I	ATGCAT	2 cut(s)	1105, 1339
MroI	TCCGGA	1 cut(s)	631
MslI	CAYNNNNRTG	4 cut(s)	1355, 1617, 1934, 2705
MspA1I	CMGCKG	1 cut(s)	126
MspI	CCGG	8 cut(s)	474, 632, 1369, 1983, 2360, 2751, 3929, 3947
MspR9I	CCNGG	7 cut(s)	354, 475, 705, 750, 2360, 2751, 3060
MunI	CAATTG	1 cut(s)	1866
Mva1269I	GAATGC	3 cut(s)	1799, 3367, 3409
MvaI	CCWGG	4 cut(s)	354, 705, 750, 3060
MvnI	CGCG	1 cut(s)	3768
MwoI	GCNNNNNNNGC	9 cut(s)	434, 1100, 1316, 2033, 2045, 2202, 2456, 3802, 3975
NciI	CCSGG	3 cut(s)	475, 2360, 2751
NdeI	CATATG	3 cut(s)	984, 1113, 2996
NlaIV	GGNNCC	5 cut(s)	293, 323, 2133, 2748, 3933
NmuCI	GTSAC	5 cut(s)	8, 1858, 2226, 3373, 3511
NsbI	TGCGCA	1 cut(s)	1665
NsiI	ATGCAT	2 cut(s)	1105, 1339
NspI	RCATGY	4 cut(s)	2027, 2349, 2448, 3388
NspV	TTCGAA	1 cut(s)	1251
PaeI	GCATGC	1 cut(s)	2027
PaeR7I	CTCGAG	2 cut(s)	80, 3018
PaqCI	CACCTGC	1 cut(s)	1660
PasI	CCCWGGG	1 cut(s)	353
PciI	ACATGT	1 cut(s)	2345
PctI	GAATGC	3 cut(s)	1799, 3367, 3409
Pfl23II	CGTACG	1 cut(s)	2853
PflFI	GACNNNGTC	1 cut(s)	191
PfoI	TCCNGGA	2 cut(s)	2358, 3058
PinAI	ACCGGT	1 cut(s)	3928
PleI	GAGTC	3 cut(s)	264, 2384, 3457
PpsI	GAGTC	3 cut(s)	264, 2384, 3457
Ppu21I	YACGTR	1 cut(s)	2853
PscI	ACATGT	1 cut(s)	2345
PshAI	GACNNNNGTC	1 cut(s)	2224
PsiI	TTATAA	3 cut(s)	2877, 3441, 3533
Psp1406I	AACGTT	2 cut(s)	2370, 3732
Psp6I	CCWGG	4 cut(s)	352, 703, 748, 3058
PspFI	CCCAGC	2 cut(s)	1124, 1502
PspGI	CCWGG	4 cut(s)	352, 703, 748, 3058
PspLI	CGTACG	1 cut(s)	2853
PspN4I	GGNNCC	5 cut(s)	293, 323, 2133, 2748, 3933
PspPI	GGNCC	2 cut(s)	2115, 3418
PspXI	VCTCGAGB	1 cut(s)	80
PstI	CTGCAG	3 cut(s)	1099, 2947, 3463
PstNI	CAGNNNCTG	1 cut(s)	3355
PsuI	RGATCY	2 cut(s)	616, 2564
PsyI	GACNNNGTC	1 cut(s)	191
PvuII	CAGCTG	1 cut(s)	126
RseI	CAYNNNNRTG	4 cut(s)	1355, 1617, 1934, 2705
Sau96I	GGNCC	2 cut(s)	2115, 3418
ScaI	AGTACT	2 cut(s)	2234, 3989
SchI	GAGTC	3 cut(s)	264, 2385, 3458
ScrFI	CCNGG	7 cut(s)	354, 475, 705, 750, 2360, 2751, 3060
SduI	GDGCHC	5 cut(s)	251, 701, 1626, 2749, 3825
SfcI	CTRYAG	3 cut(s)	1095, 2943, 3459
Sfr274I	CTCGAG	2 cut(s)	80, 3018
SfuI	TTCGAA	1 cut(s)	1251
SinI	GGWCC	2 cut(s)	2115, 3418
SlaI	CTCGAG	2 cut(s)	80, 3018
SmiMI	CAYNNNNRTG	4 cut(s)	1355, 1617, 1934, 2705
SmlI	CTYRAG	3 cut(s)	80, 2310, 3018
SmoI	CTYRAG	3 cut(s)	80, 2310, 3018
SnaBI	TACGTA	1 cut(s)	2853
SphI	GCATGC	1 cut(s)	2027
SspI	AATATT	2 cut(s)	732, 1176
SspMI	CTAG	5 cut(s)	1040, 1215, 2588, 2636, 3038
StyD4I	CCNGG	7 cut(s)	352, 473, 703, 748, 2358, 2749, 3058
StyI	CCWWGG	1 cut(s)	1214
TaiI	ACGT	8 cut(s)	1761, 2373, 2493, 2738, 2799, 2823, 2855, 3735
TatI	WGTACW	4 cut(s)	2232, 2758, 3642, 3987
TauI	GCSGC	2 cut(s)	1350, 3815
TscAI	CASTG	8 cut(s)	286, 1462, 2557, 3265, 3303, 3754, 3825, 3830
TseFI	GTSAC	5 cut(s)	8, 1858, 2226, 3373, 3511
Tsp45I	GTSAC	5 cut(s)	8, 1858, 2226, 3373, 3511
TspGWI	ACGGA	2 cut(s)	3361, 3789
TspRI	CASTG	8 cut(s)	286, 1462, 2557, 3265, 3303, 3754, 3825, 3830
Tth111I	GACNNNGTC	1 cut(s)	191
VneI	GTGCAC	1 cut(s)	3821
VpaK11BI	GGWCC	2 cut(s)	2115, 3418
XagI	CCTNNNNNAGG	1 cut(s)	2126
XapI	RAATTY	3 cut(s)	41, 1718, 2596
XceI	RCATGY	4 cut(s)	2027, 2349, 2448, 3388
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1790
XhoI	CTCGAG	2 cut(s)	80, 3018
XmaJI	CCTAGG	1 cut(s)	1214
XmiI	GTMKAC	2 cut(s)	1645, 1789
XspI	CTAG	5 cut(s)	1040, 1215, 2588, 2636, 3038
ZrmI	AGTACT	2 cut(s)	2234, 3989
Zsp2I	ATGCAT	2 cut(s)	1105, 1339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.