Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Reverse (-)

46373461 .. 46384477

Gene Structure 11017 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034622

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4584 bp

ATGGCCGGAACTAGCTTGGTCTTTGCTGTTAATCGCCAGAGGTTTCACCTCCCTACTGTTGATCCTTCTACCACTCTGCTTGAGTTTCTCCGTTCCCACACTCGCTTCAAGAGCGTCAAGCTCAGCTGTGGCGAAGGTGGTTGTGGTGCTTGTGTTGTGCTACTGTCCAAGTATGATCCTGTTCTTGACAGAGTGGAGGATTTTGCTGTCAGTTCATGTCTCACTTTACTCTGCAGCGTAAACCTATGCTCTATTACGACCTCTGAAGGTTTTGGGAATAGTAAAGATGGCTTTCATGCAATTCACCAGAGGTTCAGCGGTTTCCACGCTTCGCAATGCGGGTTTTGTACTCCGGGGATGTGTGTTTCACTCTTTTCGGCTCTTGTCAATGCTGAGAAGAAGGCCGATCGAGTGGAGGCCCCTCCCGGGTTCTCCAAGCTGACTGTGTCTGAAGCTGAGAAGGCTATTGCAGGAAACCTCTGTCGCTGTACTGGGTATCGTTCCATTGCTGATGCCTGCAAGAGCTTTTCGGCTGATGTTGACATGGAGGATTTGGGGTTCAACTCCTTTTGGAGGAAGGGAGACAGTAAGCAAGCCAAGATAGATAGTTTGCCTCCTTATAATCCTGAGAGTGAGGTTTGTACATTCCCGGAGTTTTTGAAAAATGAAATCAGGTCCTTGTCGTCGTTTTTGGATTCCAAGAGATATGGTTGGTACAGTCCAGCTACTATTGAGGAGCTGAAGAGCTTAGTGGAAGCAAATGATTCTGGTGATAGTATGAAGTTGGTTGTTGGTAACACAGGGACGGGGTATTACAAGGAGTTAACAGGTTATGACAAATACATTGATCTCAGATATGTTCCTGAACTATCAATAATTAGGATGGATCCGACGGGTCTTAGTGTAGGAGCAATTGTAACCATCACTAAAGTTATTGAAGCTTTAAAGAAGAAAACCAAAGGTGAACTTATCTCAAGAGGTGAGCTAGTGTTTGAAAGAATTGCCAGGCATATGGATAAAATTGCTTCAGGGTTTCTCAGAAATACAGCTAGTATAGGAGGGAATTTGGTGATGGCACAAAGAAAGAACTTTCCTTCAGATATTGCTACAGTACTTCTCGCTGTGGATTCAACAGTGAATATCATGAGTGGTTCTAGATCTGAAATTATTATGTTGGAGGATTTTCTGAAAAGAGCCCCATTGGATCCCAAAAGTGTACTTTTAAGTGTTAAAATCCCAAACTGGGAAGCAGTTACAACATTTTCTGCAGGATCCAATACTATGTTGCTATTTGAAACCTATAGAGCTGCACCCAGACCGCTAGGAAATGCATTGCCTTATTTGAATGCTGCTTTCTTGGCTGAAGTTTCTTCATGTAAAACTTCTACTGGATTTGTGGTACATCACTGCTGTCTGGCTTTTGGTGCTTATGGGACTAAACATGCAATTAGAGCAAGAAAGGTTGAGGACTTTTTAAACGGAAAAACCTTGAGTGCTGGTGTTTTGTATGAAGCGATTAAGTTAGTTCGAGCTACTGTGGTTCCTGAAGAAGGCACTACGAGTCCTGCTTACAGGTCAAGCTTGGCTTGTGGTTTTCTTTTTGAGTTCTTTAGCCCCTATATTAACAGTGACACTGAAATTTCTGATGGTTTCTTGGAGAATATATTGTTCCCTACTTCTAAGATGAATAAGAACCAATGTAGCAATGATGAGATTCCATCTGTTCTATCATCAGCTAAACAAGTGCTTAATTTAAGTGCAGACTATGATCCAGTCGGCAAGCCAATTATAAAATCTGGAGCTGCCATCCAAGCTTCAGGTGAGGCTGTGTATGTAGATGACATTCCGTCCCCGACAAATTGCTTGCATGGAGCGTTCATTTATAGCACAAAGCCTCTTGTACGGGTTAAGGGAATAAAATTCAAAACTAAACCACAGCCAGATGGAGTTTCAGCTGTCTTATCTTTTAAAGATATCCCCAATGGTGGGGAGAATGTGGGATCTATGACTATTTTTGGTACTGAACCTCTTTTTGCTGATGATCTTACTCAATGCGCTGGTCAGCGTCTTGCCTTTTTGGTTGCAGATACCCAGAAAAATGCAGATTTGGCTGCAAATTTTGTGGATGTTGATTATGAAATGGAAAGTATAGAGCAACCTATACTATCCGTAGAGGAGGCTATTAAGAGGTCTAGTTTTTTTGAGGTCCCTCCTTTCTTATACCCGAAGCAAGTTGGTGACATATCAAAAGGAATGGCTTTAGCTGATCACAAGATTACATCTGCTGAGATCAAACTTGGTTCACAGTATTATTTCTACATGGAGACACAAACTGCCCTTGCTGTACCAGATGAAGACAACTGCATAGTGGTTTATACTTCAAGTCAGTGTCCTCAGTTTCAACACTCAGTCATCGCAAAGTGTCTTGGCATTCCTGAAAGTAATGTCCGTGTGATTACAAGGAGGGTTGGTGGGGGTTTTGGTGGAAAGGCCGTAAAATCCATTCCTGTCGCAACTGCATGTGCACTAGCAGCACACAAATTACACCGCCCTGTCAGGATTTATGTCAATCGCAAGACTGATATGATAATGGCGGGAGGAAGGCATCCCATGAAGATAATTTACAGTGTGGGATTCAAGTCTGATGGGAAGATTACAGCTCTACAACTTGATATATTAATTGATGCAGGGATGTCCCCAGATATAAGTCCAATTCTGCCAAACAATGTTCTGGGTGCACTTAAGAAGTATGATTGGGGTGCTCTGTCTTTTGATGTAAAGGTATGCAAAACTAACAATCCAAGTAGGTCTGCTATGCGGGGTCCTGGGGAGGTGCAGGGGTCATTTATTGCTGAAGCTGTAATTGAACATGTAGCATCAACCCTTTCTATGGAAGTGGATTGTGTGAGAAATATAAATCTTCACACACATTGCAGCCTTGATTTATTTTATGAGAATAGTGCTGGTGAACCTCACGAGTATACTTTACCTTTAATTTGGGACAAGCTGGCCATGTCTTCAAGCTTTAACGAAAGGACTGAAATGGTAAAAGGATTTAATAAATGCAATAAATGGCGGAAAAGAGGAATTTCTCGAGTACCTATAGTACATGAAGTTTCATTGAGACCAACTCCAGGGAAAGTGAGCATTCTAAGCGATGGTTCTGTTGTTGTTGAAGTTGGAGGGATTGAACTGGGTCAGGGTCTCTGGACAAAAGTAAAACAGATGGCTGCTTTCGCTCTTGGTTCCATTCAATGTAATGATAGTGGAGACCTTTTGGATAAAGTACGGGTGGTACAATCTGATACAGTTAGCTTAATTCAAGGAGGATTTACTGCTGGAAGCACTACATCTGAGTCAAGTTGCCAGGCAGTAAGGTTATCCTGTGATATTTTGGTTGAGAGGCTAGCTCCTCTCAAGAAAATATTGCAGGAGCAAATGGGTTCTATTAAATGGGAGATGCTTATTGAAAAGGCGTACCTGCAAGCCTTGAATTTATCAGCAAGTTCTTTCTACGTTCCTGATTTTACTTCCATGCAATATCTCAACTATGGTGCTGCAGTGAGTGAGGTTGAAGTAGATCTTCTGACTGGAGAAACTAGAATATTGCAATCAGATATTATCTACGATTGCGGTCAGAGTCTCAACCCTGCTGTGGATTTAGGACAGATCGAAGGTGCTTTTGTTCAAGGAATTGGGTTTTTCATGCTTGAAGAATACATGGAAAATTCTGAGGGATTGGTGATTTCTAATGGTACATGGACGTATAAAATTCCTTCCATAGACACTATACCCAAGCAGTTCAATGTCGAAATACTAAACAGTGGACAACACAGTAAACGTGTTCTCTCTTCAAAAGCTTCTGGTGAGCCTCCATTACTTCTAGCAGTTTCAGTTCACTGCGCTGCAAGAGCTGCTATCAAAGAATCCAGAAAACAGCTTCTTCAATGGGGAGGCCTGGATGGGTCTGCTTCAATGTTCCAGATGGCCGTTCCAGCTACCATGCCTGTTGTGAAGGAGTTATGTGGGCTGGAAAGCGTGGAGAGCTACTTGGAATGGATTGCAGGCAGAAACAATTCTCAACATCATGATGCAGTTAGTGGCACATCTCGGCAGCATGGAGCTGATGATTATACAAAACGACTTTCAATAGGTTTCAAGGAGGTTGTCAGTGAAAATGTCACTGACAAGGACTCCACAGGAAAGAAACTTGAATCACAGATTCTACCCCTGCTTAATGCATCTGTCGGCTTCAGAAATGACCGTGTTAAGGCATACCTGGTCATAGCTCTGAATGTGACTCCAGGTTATTGGTTTGCATTTTCAGCAACTGTGCCGCCTCTTGGTTTTAGCACTTACATTGTCTCAAGTGCCTCCACACAGACAGCTACTACTTCAGAGAGACGTACAGTGTACAAGACTGAAACAAGTCAAAATGATACAATCAAAGTCGGGCCAGGAAACTTGAAACTCATTTACTCTGGAAATGATGGAAAACTAATTCAGTACAAGAACAGCAGAAGCTCAAGCAAGTCCACCGGTCTGTTCTTGACCGGCCTCCAGCTGGGATTAATGATGTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1528

Amino Acids

166.32

Weight (kDa)

6.61

Isoelectric Point (pI)

36.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	9 - 75	4.1e-07	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	87 - 174	2.1e-21	[2Fe-2S] binding domain
FAD_binding_5	PF00941	235 - 415	2.9e-39	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	433 - 538	1.2e-19	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	606 - 714	3.6e-28	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	743 - 974	8.7e-81	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1003 - 1265	9.3e-74	Molybdopterin cofactor-binding domain
Laman-like_dom	PF21260	1432 - 1455	3.5e-06	Lysosomal alpha-mannosidase-like, central domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	621, 1790
Acc36I	ACCTGC	1 cut(s)	3489
AccI	GTMKAC	1 cut(s)	2981
AciI	CCGC	9 cut(s)	318, 339, 1319, 2548, 2594, 2818, 3076, 3633, 4337
AcoI	YGGCCR	3 cut(s)	3, 3009, 3987
AcsI	RAATTY	8 cut(s)	1063, 1638, 1919, 2116, 3087, 3493, 3727, 3771
AflII	CTTAAG	1 cut(s)	2741
AflIII	ACRYGT	2 cut(s)	2869, 3841
AgeI	ACCGGT	1 cut(s)	4538
AhdI	GACNNNNNGTC	1 cut(s)	1846
AjnI	CCWGG	8 cut(s)	1004, 2824, 3133, 3366, 3957, 4278, 4303, 4456
AloI	GAACNNNNNNTCC	2 cut(s)	542, 574
Alw21I	GWGCWC	3 cut(s)	2527, 2740, 2764
Alw26I	GTCTC	9 cut(s)	224, 576, 2318, 3118, 3209, 3264, 3647, 4369, 4396
Alw44I	GTGCAC	2 cut(s)	2523, 2736
AlwNI	CAGNNNCTG	1 cut(s)	4331
Ama87I	CYCGRG	2 cut(s)	425, 3093
AoxI	GGCC	9 cut(s)	3, 402, 417, 2490, 3009, 3955, 3987, 4454, 4555
ApaLI	GTGCAC	2 cut(s)	2523, 2736
ApoI	RAATTY	8 cut(s)	1063, 1638, 1919, 2116, 3087, 3493, 3727, 3771
AseI	ATTAAT	2 cut(s)	2678, 4571
AsiGI	ACCGGT	1 cut(s)	4538
Asp700I	GAANNNNTTC	1 cut(s)	4075
AspLEI	GCGC	2 cut(s)	2057, 3905
AspS9I	GGNCC	5 cut(s)	418, 675, 2206, 2822, 4454
AsuC2I	CCSGG	4 cut(s)	354, 426, 427, 650
AsuNHI	GCTAGC	1 cut(s)	3406
AvaI	CYCGRG	2 cut(s)	425, 3093
AvaII	GGWCC	3 cut(s)	675, 2206, 2822
BaeGI	GKGCMC	2 cut(s)	2527, 2740
BalI	TGGCCA	1 cut(s)	3011
BamHI	GGATCC	3 cut(s)	886, 1204, 1271
BanII	GRGCYC	1 cut(s)	1198
BarI	GAAGNNNNNNTAC	2 cut(s)	2606, 2638
BauI	CACGAG	1 cut(s)	2975
BbsI	GAAGAC	2 cut(s)	2361, 3009
Bbv12I	GWGCWC	3 cut(s)	2527, 2740, 2764
BceAI	ACGGC	2 cut(s)	2477, 3974
BciT130I	CCWGG	8 cut(s)	1006, 2826, 3135, 3368, 3959, 4280, 4305, 4458
BclI	TGATCA	1 cut(s)	2266
BcnI	CCSGG	4 cut(s)	354, 426, 427, 650
BcoDI	GTCTC	9 cut(s)	224, 576, 2318, 3118, 3209, 3264, 3647, 4369, 4396
BfmI	CTRYAG	6 cut(s)	232, 1107, 1266, 1300, 3102, 3558
BfrI	CTTAAG	1 cut(s)	2741
BfuAI	ACCTGC	1 cut(s)	3489
BglII	AGATCT	2 cut(s)	1157, 3580
BlpI	GCTNAGC	1 cut(s)	122
BmcAI	AGTACT	1 cut(s)	1113
Bme18I	GGWCC	3 cut(s)	675, 2206, 2822
BmeRI	GACNNNNNGTC	1 cut(s)	1846
BmeT110I	CYCGRG	2 cut(s)	425, 3093
BmgT120I	GGNCC	5 cut(s)	418, 675, 2206, 2822, 4454
BmiI	GGNNCC	8 cut(s)	420, 888, 1206, 1273, 1542, 2208, 2823, 3247
BmrI	ACTGGG	3 cut(s)	501, 1252, 3203
BmsI	GCATC	7 cut(s)	502, 2614, 2674, 2885, 3450, 4081, 4250
BmtI	GCTAGC	1 cut(s)	3410
BmuI	ACTGGG	3 cut(s)	501, 1252, 3203
BpiI	GAAGAC	2 cut(s)	2361, 3009
BplI	GAGNNNNNCTC	4 cut(s)	3115, 3147, 3394, 3426
BpmI	CTGGAG	5 cut(s)	1818, 3117, 3612, 4287, 4544
Bpu1102I	GCTNAGC	1 cut(s)	122
BpuEI	CTTGAG	6 cut(s)	101, 958, 1510, 3401, 4351, 4510
BpuMI	CCSGG	4 cut(s)	354, 426, 427, 650
BsaI	GGTCTC	3 cut(s)	3118, 3209, 3264
BsaJI	CCNNGG	4 cut(s)	353, 425, 2825, 3134
BsaWI	WCCGGW	1 cut(s)	4538
BsaXI	ACNNNNNCTCC	6 cut(s)	900, 930, 2455, 2485, 4178, 4208
Bse118I	RCCGGY	2 cut(s)	4538, 4553
Bse1I	ACTGG	6 cut(s)	496, 1247, 1393, 1772, 3198, 3595
Bse3DI	GCAATG	5 cut(s)	341, 504, 1331, 1711, 2929
BseBI	CCWGG	8 cut(s)	1006, 2826, 3135, 3368, 3959, 4280, 4305, 4458
BseDI	CCNNGG	4 cut(s)	353, 425, 2825, 3134
BseGI	GGATG	7 cut(s)	363, 888, 1806, 2131, 2605, 2697, 3967
BseMI	GCAATG	5 cut(s)	341, 504, 1331, 1711, 2929
BseNI	ACTGG	6 cut(s)	496, 1247, 1393, 1772, 3198, 3595
BseRI	GAGGAG	3 cut(s)	749, 2189, 3402
BseSI	GKGCMC	2 cut(s)	2527, 2740
BseYI	CCCAGC	1 cut(s)	4564
BsgI	GTGCAG	3 cut(s)	1293, 1779, 2855
Bsh1285I	CGRYCG	1 cut(s)	409
BshFI	GGCC	9 cut(s)	5, 404, 419, 2492, 3011, 3957, 3989, 4456, 4557
BshTI	ACCGGT	1 cut(s)	4538
BsiEI	CGRYCG	1 cut(s)	409
BsiHKAI	GWGCWC	3 cut(s)	2527, 2740, 2764
BsiHKCI	CYCGRG	2 cut(s)	425, 3093
BsiSI	CCGG	6 cut(s)	6, 353, 426, 650, 4539, 4554
BslFI	GGGAC	6 cut(s)	817, 1447, 1834, 2192, 2680, 3014
BsmAI	GTCTC	9 cut(s)	224, 576, 2318, 3118, 3209, 3264, 3647, 4369, 4396
BsmBI	CGTCTC	1 cut(s)	4396
BsmFI	GGGAC	6 cut(s)	817, 1447, 1834, 2192, 2680, 3014
BsmI	GAATGC	3 cut(s)	1351, 2430, 3147
BsnI	GGCC	9 cut(s)	5, 404, 419, 2492, 3011, 3957, 3989, 4456, 4557
Bso31I	GGTCTC	3 cut(s)	3118, 3209, 3264
BsoBI	CYCGRG	2 cut(s)	425, 3093
Bsp1286I	GDGCHC	4 cut(s)	1198, 2527, 2740, 2764
Bsp1407I	TGTACA	2 cut(s)	641, 4413
Bsp1720I	GCTNAGC	1 cut(s)	122
BspACI	CCGC	9 cut(s)	318, 339, 1319, 2548, 2594, 2818, 3076, 3633, 4337
BspANI	GGCC	9 cut(s)	5, 404, 419, 2492, 3011, 3957, 3989, 4456, 4557
BspHI	TCATGA	2 cut(s)	1143, 4087
BspLI	GGNNCC	8 cut(s)	420, 888, 1206, 1273, 1542, 2208, 2823, 3247
BspMAI	CTGCAG	3 cut(s)	236, 1270, 3562
BspMI	ACCTGC	1 cut(s)	3489
BspOI	GCTAGC	1 cut(s)	3410
BspQI	GCTCTTC	1 cut(s)	737
BspTI	CTTAAG	1 cut(s)	2741
BspTNI	GGTCTC	3 cut(s)	3118, 3209, 3264
BsrDI	GCAATG	5 cut(s)	341, 504, 1331, 1711, 2929
BsrFI	RCCGGY	2 cut(s)	4538, 4553
BsrGI	TGTACA	2 cut(s)	641, 4413
BsrI	ACTGG	6 cut(s)	496, 1247, 1393, 1772, 3198, 3595
BssAI	RCCGGY	2 cut(s)	4538, 4553
BssECI	CCNNGG	4 cut(s)	353, 425, 2825, 3134
BssNAI	GTATAC	1 cut(s)	2982
BssSI	CACGAG	1 cut(s)	2975
Bst1107I	GTATAC	1 cut(s)	2982
Bst2BI	CACGAG	1 cut(s)	2975
Bst2UI	CCWGG	8 cut(s)	1006, 2826, 3135, 3368, 3959, 4280, 4305, 4458
Bst6I	CTCTTC	2 cut(s)	737, 3856
BstAFI	CTTAAG	1 cut(s)	2741
BstAPI	GCANNNNNTGC	1 cut(s)	3914
BstAUI	TGTACA	2 cut(s)	641, 4413
BstC8I	GCNNGC	8 cut(s)	517, 594, 1781, 1865, 3009, 3408, 3486, 4066
BstENI	CCTNNNNNAGG	2 cut(s)	571, 1548
BstF5I	GGATG	7 cut(s)	363, 888, 1806, 2131, 2605, 2697, 3967
BstHHI	GCGC	2 cut(s)	2057, 3905
BstMAI	GTCTC	9 cut(s)	224, 576, 2318, 3118, 3209, 3264, 3647, 4369, 4396
BstMCI	CGRYCG	1 cut(s)	409
BstNI	CCWGG	8 cut(s)	1006, 2826, 3135, 3368, 3959, 4280, 4305, 4458
BstNSI	RCATGY	3 cut(s)	1445, 2523, 2873
BstSFI	CTRYAG	6 cut(s)	232, 1107, 1266, 1300, 3102, 3558
BstSLI	GKGCMC	2 cut(s)	2527, 2740
BstV2I	GAAGAC	2 cut(s)	2361, 3009
BstX2I	RGATCY	6 cut(s)	886, 1157, 1204, 1271, 2000, 3580
BstXI	CCANNNNNNTGG	2 cut(s)	1012, 4311
BstYI	RGATCY	6 cut(s)	886, 1157, 1204, 1271, 2000, 3580
BstZ17I	GTATAC	1 cut(s)	2982
BsuRI	GGCC	9 cut(s)	5, 404, 419, 2492, 3011, 3957, 3989, 4456, 4557
BtgZI	GCGATG	2 cut(s)	2398, 3171
BtsCI	GGATG	7 cut(s)	363, 888, 1806, 2131, 2605, 2697, 3967
BtsI	GCAGTG	3 cut(s)	1405, 3567, 3898
BveI	ACCTGC	1 cut(s)	3489
Cac8I	GCNNGC	8 cut(s)	517, 594, 1781, 1865, 3009, 3408, 3486, 4066
CaiI	CAGNNNCTG	1 cut(s)	4331
CciI	TCATGA	2 cut(s)	1143, 4087
CfoI	GCGC	2 cut(s)	2057, 3905
Cfr10I	RCCGGY	2 cut(s)	4538, 4553
Cfr13I	GGNCC	5 cut(s)	418, 675, 2206, 2822, 4454
Cfr9I	CCCGGG	1 cut(s)	425
CseI	GACGC	2 cut(s)	103, 2054
CsiI	ACCWGGT	1 cut(s)	4278
CspAI	ACCGGT	1 cut(s)	4538
CspCI	CAANNNNNGTGG	4 cut(s)	61, 96, 2103, 2138
DraI	TTTAAA	3 cut(s)	945, 1476, 1969
DriI	GACNNNNNGTC	1 cut(s)	1846
EaeI	YGGCCR	3 cut(s)	3, 3009, 3987
Eam1104I	CTCTTC	2 cut(s)	737, 3856
Eam1105I	GACNNNNNGTC	1 cut(s)	1846
EarI	CTCTTC	2 cut(s)	737, 3856
EciI	GGCGGA	1 cut(s)	3091
Eco147I	AGGCCT	1 cut(s)	3957
Eco24I	GRGCYC	1 cut(s)	1198
Eco31I	GGTCTC	3 cut(s)	3118, 3209, 3264
Eco32I	GATATC	1 cut(s)	1975
Eco47I	GGWCC	3 cut(s)	675, 2206, 2822
Eco88I	CYCGRG	2 cut(s)	425, 3093
EcoNI	CCTNNNNNAGG	2 cut(s)	571, 1548
EcoO109I	RGGNCCY	4 cut(s)	418, 675, 2206, 2822
EcoRII	CCWGG	8 cut(s)	1004, 2824, 3133, 3366, 3957, 4278, 4303, 4456
EcoRV	GATATC	1 cut(s)	1975
EcoT22I	ATGCAT	2 cut(s)	1333, 4243
EcoT38I	GRGCYC	1 cut(s)	1198
Esp3I	CGTCTC	1 cut(s)	4396
FalI	AAGNNNNNCTT	6 cut(s)	951, 983, 1570, 1602, 3567, 3599
FaqI	GGGAC	6 cut(s)	817, 1447, 1834, 2192, 2680, 3014
FauI	CCCGC	3 cut(s)	332, 2587, 2811
FauNDI	CATATG	1 cut(s)	1011
FbaI	TGATCA	1 cut(s)	2266
FblI	GTMKAC	1 cut(s)	2981
FokI	GGATG	7 cut(s)	370, 895, 1793, 2138, 2592, 2704, 3974
FriOI	GRGCYC	1 cut(s)	1198
GlaI	GCGC	2 cut(s)	2056, 3904
GsaI	CCCAGC	1 cut(s)	4568
GsuI	CTGGAG	5 cut(s)	1818, 3117, 3612, 4287, 4544
HaeIII	GGCC	9 cut(s)	5, 404, 419, 2492, 3011, 3957, 3989, 4456, 4557
HapII	CCGG	6 cut(s)	6, 353, 426, 650, 4539, 4554
HgaI	GACGC	2 cut(s)	103, 2054
HhaI	GCGC	2 cut(s)	2057, 3905
Hin6I	GCGC	2 cut(s)	2055, 3903
HinP1I	GCGC	2 cut(s)	2055, 3903
HincII	GTYRAC	2 cut(s)	541, 825
HindII	GTYRAC	2 cut(s)	541, 825
HindIII	AAGCTT	5 cut(s)	939, 1579, 1812, 3022, 3858
HpaI	GTTAAC	1 cut(s)	825
HpaII	CCGG	6 cut(s)	6, 353, 426, 650, 4539, 4554
Hpy99I	CGWCG	2 cut(s)	688, 895
HpyCH4IV	ACGT	4 cut(s)	3516, 3764, 3841, 4405
HpySE526I	ACGT	4 cut(s)	3516, 3764, 3841, 4405
HspAI	GCGC	2 cut(s)	2055, 3903
Ksp22I	TGATCA	1 cut(s)	2266
KspAI	GTTAAC	1 cut(s)	825
LguI	GCTCTTC	1 cut(s)	737
LmnI	GCTCC	7 cut(s)	736, 908, 1799, 1871, 3415, 3433, 4121
LweI	GCATC	7 cut(s)	502, 2614, 2674, 2885, 3450, 4081, 4250
MabI	ACCWGGT	1 cut(s)	4278
MaeII	ACGT	4 cut(s)	3516, 3764, 3841, 4405
MaeIII	GTNAC	7 cut(s)	794, 916, 1252, 1628, 2237, 4180, 4297
MfeI	CAATTG	1 cut(s)	912
MflI	RGATCY	6 cut(s)	886, 1157, 1204, 1271, 2000, 3580
MhlI	GDGCHC	4 cut(s)	1198, 2527, 2740, 2764
MlsI	TGGCCA	1 cut(s)	3011
MluNI	TGGCCA	1 cut(s)	3011
MlyI	GAGTC	5 cut(s)	1570, 3365, 3649, 4187, 4294
MmeI	TCCRAC	3 cut(s)	914, 1155, 3160
Mox20I	TGGCCA	1 cut(s)	3011
Mph1103I	ATGCAT	2 cut(s)	1333, 4243
MroXI	GAANNNNTTC	1 cut(s)	4075
MscI	TGGCCA	1 cut(s)	3011
MslI	CAYNNNNRTG	1 cut(s)	4089
Msp20I	TGGCCA	1 cut(s)	3011
MspA1I	CMGCKG	4 cut(s)	126, 318, 1955, 4564
MspCI	CTTAAG	1 cut(s)	2741
MspI	CCGG	6 cut(s)	6, 353, 426, 650, 4539, 4554
MunI	CAATTG	1 cut(s)	912
Mva1269I	GAATGC	3 cut(s)	1351, 2430, 3147
MvaI	CCWGG	8 cut(s)	1006, 2826, 3135, 3368, 3959, 4280, 4305, 4458
NciI	CCSGG	4 cut(s)	354, 426, 427, 650
NdeI	CATATG	1 cut(s)	1011
NheI	GCTAGC	1 cut(s)	3406
NlaIV	GGNNCC	8 cut(s)	420, 888, 1206, 1273, 1542, 2208, 2823, 3247
NmeAIII	GCCGAG	1 cut(s)	4090
NmuCI	GTSAC	4 cut(s)	1628, 2237, 4180, 4297
NsiI	ATGCAT	2 cut(s)	1333, 4243
NspI	RCATGY	3 cut(s)	1445, 2523, 2873
PaeR7I	CTCGAG	1 cut(s)	3093
PagI	TCATGA	2 cut(s)	1143, 4087
PceI	AGGCCT	1 cut(s)	3957
PciI	ACATGT	1 cut(s)	2869
PciSI	GCTCTTC	1 cut(s)	737
PctI	GAATGC	3 cut(s)	1351, 2430, 3147
PdmI	GAANNNNTTC	1 cut(s)	4075
PfeI	GAWTC	8 cut(s)	695, 764, 1127, 1714, 2634, 3926, 4214, 4222
PflFI	GACNNNGTC	1 cut(s)	445
PfoI	TCCNGGA	1 cut(s)	648
PinAI	ACCGGT	1 cut(s)	4538
Ple19I	CGATCG	1 cut(s)	409
PleI	GAGTC	5 cut(s)	1569, 3364, 3648, 4187, 4294
PpsI	GAGTC	5 cut(s)	1569, 3364, 3648, 4187, 4294
PpuMI	RGGWCCY	3 cut(s)	675, 2206, 2822
PscI	ACATGT	1 cut(s)	2869
PshBI	ATTAAT	2 cut(s)	2678, 4571
PsiI	TTATAA	2 cut(s)	621, 1790
Psp5II	RGGWCCY	3 cut(s)	675, 2206, 2822
Psp6I	CCWGG	8 cut(s)	1004, 2824, 3133, 3366, 3957, 4278, 4303, 4456
PspFI	CCCAGC	1 cut(s)	4564
PspGI	CCWGG	8 cut(s)	1004, 2824, 3133, 3366, 3957, 4278, 4303, 4456
PspN4I	GGNNCC	8 cut(s)	420, 888, 1206, 1273, 1542, 2208, 2823, 3247
PspPI	GGNCC	5 cut(s)	418, 675, 2206, 2822, 4454
PspPPI	RGGWCCY	3 cut(s)	675, 2206, 2822
PstI	CTGCAG	3 cut(s)	236, 1270, 3562
PstNI	CAGNNNCTG	1 cut(s)	4331
PsuI	RGATCY	6 cut(s)	886, 1157, 1204, 1271, 2000, 3580
PsyI	GACNNNGTC	1 cut(s)	445
PvuI	CGATCG	1 cut(s)	409
PvuII	CAGCTG	3 cut(s)	126, 1955, 4564
RseI	CAYNNNNRTG	1 cut(s)	4089
SapI	GCTCTTC	1 cut(s)	737
Sau96I	GGNCC	5 cut(s)	418, 675, 2206, 2822, 4454
ScaI	AGTACT	1 cut(s)	1113
SchI	GAGTC	5 cut(s)	1570, 3365, 3649, 4187, 4294
SduI	GDGCHC	4 cut(s)	1198, 2527, 2740, 2764
SexAI	ACCWGGT	1 cut(s)	4278
SfaNI	GCATC	7 cut(s)	502, 2614, 2674, 2885, 3450, 4081, 4250
SfcI	CTRYAG	6 cut(s)	232, 1107, 1266, 1300, 3102, 3558
Sfr274I	CTCGAG	1 cut(s)	3093
SinI	GGWCC	3 cut(s)	675, 2206, 2822
SlaI	CTCGAG	1 cut(s)	3093
SmaI	CCCGGG	1 cut(s)	427
SmiMI	CAYNNNNRTG	1 cut(s)	4089
SmlI	CTYRAG	8 cut(s)	80, 973, 1489, 2741, 3093, 3416, 4366, 4525
SmoI	CTYRAG	8 cut(s)	80, 973, 1489, 2741, 3093, 3416, 4366, 4525
SseBI	AGGCCT	1 cut(s)	3957
SsiI	CCGC	9 cut(s)	318, 339, 1319, 2548, 2594, 2818, 3076, 3633, 4337
SspI	AATATT	2 cut(s)	3426, 3606
StuI	AGGCCT	1 cut(s)	3957
TaiI	ACGT	4 cut(s)	3519, 3767, 3844, 4408
TaqI	TCGA	5 cut(s)	409, 1528, 3094, 3672, 3810
TatI	WGTACW	8 cut(s)	347, 488, 641, 1111, 1216, 3106, 4413, 4506
TauI	GCSGC	1 cut(s)	4339
TfiI	GAWTC	8 cut(s)	695, 764, 1127, 1714, 2634, 3926, 4214, 4222
TseFI	GTSAC	4 cut(s)	1628, 2237, 4180, 4297
Tsp45I	GTSAC	4 cut(s)	1628, 2237, 4180, 4297
TspGWI	ACGGA	5 cut(s)	80, 1494, 1836, 2158, 2438
TspMI	CCCGGG	1 cut(s)	425
Tth111I	GACNNNGTC	1 cut(s)	445
Vha464I	CTTAAG	1 cut(s)	2741
VneI	GTGCAC	2 cut(s)	2523, 2736
VpaK11BI	GGWCC	3 cut(s)	675, 2206, 2822
VspI	ATTAAT	2 cut(s)	2678, 4571
XagI	CCTNNNNNAGG	2 cut(s)	571, 1548
XapI	RAATTY	8 cut(s)	1063, 1638, 1919, 2116, 3087, 3493, 3727, 3771
XbaI	TCTAGA	1 cut(s)	1154
XceI	RCATGY	3 cut(s)	1445, 2523, 2873
XhoI	CTCGAG	1 cut(s)	3093
XmaI	CCCGGG	1 cut(s)	425
XmiI	GTMKAC	1 cut(s)	2981
XmnI	GAANNNNTTC	1 cut(s)	4075
ZrmI	AGTACT	1 cut(s)	1113
Zsp2I	ATGCAT	2 cut(s)	1333, 4243

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.