Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Reverse (-)

19588103 .. 19596342

Gene Structure 8240 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1162200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4134 bp

ATGGCCGACGAAAGAGAAGCAGCAGGAAGCGGTGGCAGCCTGGTTTTTGCAGTTAACGGGGAGAGGTTTGAGCTCTCGACCCTTGACCCTTCCACCACTCTCCTCGACTTCTTGCGTTCTCACACCACATTCAAAAGTGTCAAGCTCAGCTGCGGCGAAGGTCGTGGTTGCGGTGCTTGTGTTGTGCTTCTGTCCAAGTACGACCCTGTTGCGGACCAAGTGAAAGATTTTACTGTAAGTTCATGTCTCACTCTGCTTTGCAGTGTAAATGGAGGTTCAATTACGACATCCGAAGGCCTTGGAAATCTCAAAGACGGGTTCCACCCAATTCACCAAAGGATTACTGGTTTCCATGCTTCTCAATGTGGCTTTTGTACTCCTGGCATGTGTGTTTCACTCTTTGGAGCTCTGGTCAATGCTAAAAAGACGAATAGACCTGAACCCCCTCATGGGTTCTCCAAGCTGACTGTTTCCGAAGCTGAAAAGTCTATTGCAGGTAACCTCTGTCGATGTACTGGGTATCGATCAATTGCTGATGCCTGCAAGAGTTTCGCAGCTGATGTTGATATGGAGGATTTGGGGTTCAACTCTTTTTGGAACAAGGGAGAGAGTAAGGAAGTAAAGATAAATAGATTGCCCCTATATAACCATAACGATGACATCTGTACATTTCCGGACTTTTTAAAAAATGAAATCCGTTCTTCTATGTCTTTGGATCCAAAGAGATATTGCTGGTACAGTCCTGTTAGAGTTGAGGAGCTTCAAAACTTATTGACAGCTACTGATTTTGACAATGCAGACGATATGAAATTAGTTGTTGGAAACACAGGGACGGGCTATTACAAGGAGCTAAAGCGCTATGACAGATACATTGATCTCAGATATGTTCCTGAATTATCAATGATTAAGATAGATCCGACAGGGGTTGAGTTTGGAGCAATTGTAACAATCTCTAAGGTTATTGAAGCTTTGAGGAAGAAGGACAACGGTGAATCTCCATCAAGAGGTGAGGTTGTGTTGAAAGAAATTGCCAATCATATGGGGAAAATTGCTTCAGGGTTCATCAGAAATACAGCTAGTATAGGAGGGAATTTGGTGATGGCACAGAGAAAATGCTTTCCTTCAGATATTGCTACAATACTTCTTGCTGTGGATTCAGAGGTAAATATAATGGATGGTTCTAGATCTGAAACGATTATGTTGGAGGATTTTCTGAAACAGTCTCCACTGGATCCAAAAAGTGTACTTCTAAGTGTTAAAATCCCAAAATGGGAAGCAGTTGGAAACGTTACCAACACCGTATTGCTGTTTGAAACCTATCGAGCTGCACCAAGACCCCTAGGAAACGCATTGGCTTATTTAAATGCTGCCTTCCTAGCTGAAGTATCGTTTTGTAAGATTTCCAATGGAATTATGGTGGATAATTGTCGTCTGGCTTTTGGTGCTTATGGAACTAAACATGCAATTAGAGCAAGGAAGGTTGAGGAAATTTTAACTGGGAAAGTGCTGAGTCCTGGTGTTCTGTATGACGCTATTAAATTAGTTAAAGATGTTGTCGTTCCCGAAGAAGGAACTACTAGCCCTGCTTACAGGTCAAGCTTGGCTGCTGGTTTTCTTTTCGAGTTCTTTAGCCCCTTAATTGACAGTGAATATGATATTTCCAATGGTTTCTTGGGAACTACTTTGTTGGCCGATGCTTCTAAGTTAAAAAGGAACCAAGGTGCTAATGATAAGATGACAACTGTTCTGTCATCTGCTAAACAGGTGCTTGAATTAGGTACAGAGTATGATCCGGTTGGTAAGCCAATTACAAAATCTGGAGGTCTCATACAAGCTTCAGGTGAGGCAGTCTATGTAGACGACATTCCATCTCCGAAAAATTGTTTACATGGAGCGTTCATTTATAGTACAAAGCCTTTGGCTAGAGTGAAGGGAATAAATTTTGAACCTAAACGACATCCAGGAGTTGCTGCACTTATATCTTTAAAAGATATTCCCAAGTCTGGGGAGAACATAGGGTCTAAGACTATATTTGGTACAGAACCTCTTTTCGCCGATGATCTTACCGAATGTGCTGGTCAGCGTCTTGCTTTCGTGGTAGCCGATACGCAGAAACATGCAGACTTGGCTACAAACTTTGTGGTGGTTGATTATGACATGGAAGATATAGACCCACCTATTCTATCAGTCGAAGAGGCAGTTAAGAGATCTAATTTTTTTGAGGTCCCTCCTTTCTTGTATCCGAAACAGGTTGGTGACATATCAAATGGAATGGCTGCAGCTGATCGCAAGATTATTTCTGCTGAGATCAAACTTGGTTCACAATACTATTTCTACATGGAGACACAAACCGCCCTTGCTGTGCCAGATGAAGACAACTGCATGGTGGTTTACACTTCAAGTCAGTGCCCTGAGATTGCACACTCTTCAATTGCAAAATGCCTTGGTATTCCTGAAAATAATGTTCGTGTAATTACAAGAAGGGTTGGAGGGGGCTTTGGTGGAAAGGCCATAAAGTCCATGCCTGTGGCAACTGCATGTGCTCTTGCAGCACACCAATTACATAGACCTGTAAGGATGTATCTCAATCGGAAGACTGATATGATAATGGCTGGAGGAAGGCATCCCATGAAGATAACTTACAGTGTGGGATTCAAGTCTGATGGGAAGATTACTGCTCTAGATCTTGAGATATTAATAAATGCAGGGATTTCCCTAGATATAAGTCCAATAATGCCAAACAATATTCTGTCTGCACTTAAGAAGTATGACTGGGGTGCTCTAGCTTTCGATTTCAAGGTGTGTAAAACAAACACTCCGAGTAGGTCTGCTATGCGGGCCCCTGGGGAGGTGCAGGGGTCTTTTATTGCTGAAGCTGTAATTGAACATGTAGCATCCACCCTTTCTATAGAAGTGGATTCTGTGAGAAGCATTAATCTTCACACCTCTCACAGCCTTGATTTGTTTTATGAGCACAGCGCTGGTGAACCTCTGGAGTATACTTTACCATTGATATGGGACAAGTTGGCCATGTCTTCAAGCTTTAACCCAAGGACTGAGATGGTAAAAGAATTTAATAGGTGTAATAAATGGCAGAAAAGAGGAATTTCTCGAGTACCGATATTGCATGAAGTGACACTGAGGCCAACTCCAGCAAGAGTAAGCATTTTAGGTGATGGGTCTGTTGTCGTTGAAGTTGGAGGAATTGAACTGGGTCAGGGGCTCTGGACGAAGGTAAAACAGATGGCAGCTTTTGCTCTTGGTTCAATTCAATGTGATGGTAGTGGAGACCTTTTGGATAAACTACGGGTGGTGCAATCTGATACATTGAGCTTAATTCAAGGGGGATTTACTTCCGGGAGCACTACATCTGAGGCAAGCTGTGAGGCAGTTAGACTTTGCTGTAATATTTTGGTTGAGAGGTTAGCTACTCTCAAGGGGAGATTGAAGGAGCAAATGGGCTCTATTAACTGGGAAACTCTTATTCAGCAGGCATCCTTGGAAGCTGTGAACTTATCAGCAAGTTCTTACTATGTCCCTGATTTTGCTTCCATGAAGTACCTTAACTATGGCGCTGCTGTGAGTGAGGTGGAGGTAAATGTCCTGACTGGAGAAACCAGAATTCTGCGATCAGATATTATCTACGATTGTGGTCAGAGTCTGAACCCGGCTGTGGACTTAGGACAGATCGAAGGTGCTTTTGTTCAAGGTATTGGGTTTTTCATGCTTGAAGAATACTCGGAAAACTCAGATGGGTTGGTGATTTCAGAAGGTACGTGGACTTATAAAATTCCTACAATGGACACCATACCCAAACAGTTCAACGTTGAAGTATTGAACAGCGGGCATCACAAAAAGCGTGTGCTCTCTTCAAAAGCTTCTGGTGAGCCACCATTACTTCTTGCGGTTTCTGTTCACTGTGCTACAAGGGCTGCCATTAAAGAATCAAGAAAACAGCTTCTCCAGTGGGGCGGCTTAGATGGATCAGCTTCGATATTCCAGTTGGATGTCCCCGCTACCATGCCGGTTGTGAAGGAGCTGTGTGGGCTGGAAGCTGTGGAGAGGTACTTGGAATGGATCACTGGCAGAAACCAAGGTTCACTCGACCGGCAAATGGTTCGTCATTCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1378

Amino Acids

150.09

Weight (kDa)

5.99

Isoelectric Point (pI)

36.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	17 - 85	2.7e-06	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	96 - 182	1.4e-20	[2Fe-2S] binding domain
FAD_binding_5	PF00941	242 - 424	4.7e-40	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	439 - 544	1.9e-19	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	613 - 721	1.7e-29	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	749 - 980	1.3e-82	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1010 - 1271	1.6e-71	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3786
Acc36I	ACCTGC	1 cut(s)	485
AccI	GTMKAC	2 cut(s)	1857, 2999
AccIII	TCCGGA	1 cut(s)	673
AclI	AACGTT	2 cut(s)	1287, 3825
AclWI	GGATC	8 cut(s)	710, 723, 908, 1226, 1239, 1784, 3991, 4085
AcoI	YGGCCR	3 cut(s)	3, 1689, 3027
AcsI	RAATTY	7 cut(s)	1090, 1488, 1939, 3071, 3105, 3621, 3789
AcuI	CTGAAG	5 cut(s)	1038, 1107, 1401, 1821, 2892
AfeI	AGCGCT	2 cut(s)	857, 2980
AflII	CTTAAG	1 cut(s)	2759
AflIII	ACRYGT	1 cut(s)	2887
AjnI	CCWGG	5 cut(s)	39, 379, 1513, 1960, 2842
AloI	GAACNNNNNNTCC	2 cut(s)	566, 598
Alw21I	GWGCWC	7 cut(s)	75, 409, 2545, 2782, 2976, 3365, 3867
Alw26I	GTCTC	5 cut(s)	251, 1227, 1829, 2336, 3282
AlwI	GGATC	8 cut(s)	710, 723, 908, 1226, 1239, 1784, 3991, 4085
AlwNI	CAGNNNCTG	2 cut(s)	782, 3661
Ama87I	CYCGRG	1 cut(s)	3111
Aor13HI	TCCGGA	1 cut(s)	673
Aor51HI	AGCGCT	2 cut(s)	857, 2980
AoxI	GGCC	7 cut(s)	3, 295, 1689, 2508, 2838, 3027, 3143
ApaI	GGGCCC	1 cut(s)	2842
ApoI	RAATTY	7 cut(s)	1090, 1488, 1939, 3071, 3105, 3621, 3789
ArsI	GACNNNNNNTTYG	1 cut(s)	4105
AseI	ATTAAT	2 cut(s)	2696, 2934
AspA2I	CCTAGG	1 cut(s)	1339
AspLEI	GCGC	3 cut(s)	858, 2981, 3575
AspS9I	GGNCC	4 cut(s)	214, 2224, 2838, 2839
AsuC2I	CCSGG	2 cut(s)	3358, 3668
AvaI	CYCGRG	1 cut(s)	3111
AvaII	GGWCC	2 cut(s)	214, 2224
AvrII	CCTAGG	1 cut(s)	1339
BaeGI	GKGCMC	2 cut(s)	2411, 2842
BalI	TGGCCA	1 cut(s)	3029
BamHI	GGATCC	2 cut(s)	715, 1231
BanII	GRGCYC	5 cut(s)	75, 409, 2842, 3225, 3464
BarI	GAAGNNNNNNTAC	2 cut(s)	2624, 2656
BbsI	GAAGAC	3 cut(s)	2379, 2600, 3027
Bbv12I	GWGCWC	7 cut(s)	75, 409, 2545, 2782, 2976, 3365, 3867
BcgI	CGANNNNNNTGC	6 cut(s)	191, 225, 532, 566, 4100, 4134
BciT130I	CCWGG	5 cut(s)	41, 381, 1515, 1962, 2844
BciVI	GTATCC	1 cut(s)	2250
BcnI	CCSGG	2 cut(s)	3358, 3668
BcoDI	GTCTC	5 cut(s)	251, 1227, 1829, 2336, 3282
BfaI	CTAG	9 cut(s)	1077, 1182, 1340, 1376, 1578, 1923, 2681, 2717, 2783
BfmI	CTRYAG	2 cut(s)	2277, 2907
BfoI	RGCGCY	3 cut(s)	859, 2982, 3576
BfrI	CTTAAG	1 cut(s)	2759
BfuAI	ACCTGC	1 cut(s)	485
BfuI	GTATCC	1 cut(s)	2250
BglII	AGATCT	3 cut(s)	1184, 2207, 2683
BlnI	CCTAGG	1 cut(s)	1339
BlpI	GCTNAGC	1 cut(s)	146
Bme1390I	CCNGG	7 cut(s)	41, 381, 1515, 1962, 2844, 3358, 3668
Bme18I	GGWCC	2 cut(s)	214, 2224
BmeT110I	CYCGRG	1 cut(s)	3111
BmgT120I	GGNCC	4 cut(s)	214, 2224, 2838, 2839
BmiI	GGNNCC	7 cut(s)	320, 717, 1233, 1715, 2226, 2840, 2841
BmrFI	CCNGG	7 cut(s)	41, 381, 1515, 1962, 2844, 3358, 3668
BmrI	ACTGGG	5 cut(s)	525, 1506, 2782, 3221, 3481
BmsI	GCATC	6 cut(s)	526, 1684, 2632, 2903, 3503, 3856
BmuI	ACTGGG	5 cut(s)	525, 1506, 2782, 3221, 3481
BpiI	GAAGAC	3 cut(s)	2379, 2600, 3027
BplI	GAGNNNNNCTC	2 cut(s)	3133, 3165
BpmI	CTGGAG	6 cut(s)	1839, 2634, 3014, 3135, 3630, 3947
Bpu1102I	GCTNAGC	1 cut(s)	146
BpuEI	CTTGAG	2 cut(s)	2708, 3419
BpuMI	CCSGG	2 cut(s)	3358, 3668
Bsa29I	ATCGAT	1 cut(s)	523
BsaAI	YACGTR	1 cut(s)	3777
BsaI	GGTCTC	2 cut(s)	1829, 3282
BsaJI	CCNNGG	9 cut(s)	298, 1339, 1717, 2443, 2842, 2843, 3050, 3498, 4093
BsaWI	WCCGGW	2 cut(s)	673, 1792
BsaXI	ACNNNNNCTCC	8 cut(s)	396, 426, 927, 957, 2800, 2830, 3945, 3975
Bse118I	RCCGGY	2 cut(s)	4024, 4107
BseAI	TCCGGA	1 cut(s)	673
BseBI	CCWGG	5 cut(s)	41, 381, 1515, 1962, 2844
BseCI	ATCGAT	1 cut(s)	523
BseDI	CCNNGG	9 cut(s)	298, 1339, 1717, 2443, 2842, 2843, 3050, 3498, 4093
BseGI	GGATG	8 cut(s)	287, 1180, 1957, 2583, 2623, 2894, 3494, 4012
BseMII	CTCAG	9 cut(s)	160, 892, 1499, 2295, 2403, 3048, 3131, 3363, 3762
BseRI	GAGGAG	2 cut(s)	92, 770
BseSI	GKGCMC	2 cut(s)	2411, 2842
BsgI	GTGCAG	4 cut(s)	1311, 1956, 2739, 2873
Bsh1285I	CGRYCG	1 cut(s)	4108
BshFI	GGCC	7 cut(s)	5, 297, 1691, 2510, 2840, 3029, 3145
BshVI	ATCGAT	1 cut(s)	523
BsiEI	CGRYCG	1 cut(s)	4108
BsiHKAI	GWGCWC	7 cut(s)	75, 409, 2545, 2782, 2976, 3365, 3867
BsiHKCI	CYCGRG	1 cut(s)	3111
BsiSI	CCGG	6 cut(s)	674, 1793, 3357, 3668, 4025, 4108
BslFI	GGGAC	5 cut(s)	844, 2210, 3032, 3521, 3995
BsmAI	GTCTC	5 cut(s)	251, 1227, 1829, 2336, 3282
BsmFI	GGGAC	5 cut(s)	844, 2210, 3032, 3521, 3995
BsnI	GGCC	7 cut(s)	5, 297, 1691, 2510, 2840, 3029, 3145
Bso31I	GGTCTC	2 cut(s)	1829, 3282
BsoBI	CYCGRG	1 cut(s)	3111
Bsp120I	GGGCCC	1 cut(s)	2838
Bsp13I	TCCGGA	1 cut(s)	673
Bsp1407I	TGTACA	1 cut(s)	665
Bsp1720I	GCTNAGC	1 cut(s)	146
BspANI	GGCC	7 cut(s)	5, 297, 1691, 2510, 2840, 3029, 3145
BspCNI	CTCAG	9 cut(s)	159, 891, 1500, 2296, 2404, 3049, 3132, 3364, 3761
BspDI	ATCGAT	1 cut(s)	523
BspEI	TCCGGA	1 cut(s)	673
BspLI	GGNNCC	7 cut(s)	320, 717, 1233, 1715, 2226, 2840, 2841
BspMAI	CTGCAG	1 cut(s)	2281
BspMI	ACCTGC	1 cut(s)	485
BspPI	GGATC	8 cut(s)	710, 723, 908, 1226, 1239, 1784, 3991, 4085
BspTI	CTTAAG	1 cut(s)	2759
BspTNI	GGTCTC	2 cut(s)	1829, 3282
BsrFI	RCCGGY	2 cut(s)	4024, 4107
BsrGI	TGTACA	1 cut(s)	665
BssAI	RCCGGY	2 cut(s)	4024, 4107
BssECI	CCNNGG	9 cut(s)	298, 1339, 1717, 2443, 2842, 2843, 3050, 3498, 4093
BssNAI	GTATAC	1 cut(s)	3000
BssT1I	CCWWGG	7 cut(s)	298, 1339, 1717, 2443, 3050, 3498, 4093
Bst1107I	GTATAC	1 cut(s)	3000
Bst2UI	CCWGG	5 cut(s)	41, 381, 1515, 1962, 2844
Bst6I	CTCTTC	3 cut(s)	2187, 2431, 3874
BstAFI	CTTAAG	1 cut(s)	2759
BstAPI	GCANNNNNTGC	1 cut(s)	3254
BstAUI	TGTACA	1 cut(s)	665
BstBAI	YACGTR	1 cut(s)	3777
BstC8I	GCNNGC	5 cut(s)	541, 2838, 3379, 3492, 3845
BstEII	GGTNACC	1 cut(s)	497
BstF5I	GGATG	8 cut(s)	287, 1180, 1957, 2583, 2623, 2894, 3494, 4012
BstH2I	RGCGCY	3 cut(s)	859, 2982, 3576
BstHHI	GCGC	3 cut(s)	858, 2981, 3575
BstMAI	GTCTC	5 cut(s)	251, 1227, 1829, 2336, 3282
BstMCI	CGRYCG	1 cut(s)	4108
BstNI	CCWGG	5 cut(s)	41, 381, 1515, 1962, 2844
BstNSI	RCATGY	5 cut(s)	388, 1463, 2120, 2541, 2891
BstPI	GGTNACC	1 cut(s)	497
BstSCI	CCNGG	7 cut(s)	39, 379, 1513, 1960, 2842, 3356, 3666
BstSFI	CTRYAG	2 cut(s)	2277, 2907
BstSLI	GKGCMC	2 cut(s)	2411, 2842
BstV2I	GAAGAC	3 cut(s)	2379, 2600, 3027
BstX2I	RGATCY	6 cut(s)	715, 913, 1184, 1231, 2207, 2683
BstXI	CCANNNNNNTGG	3 cut(s)	1039, 2527, 3015
BstYI	RGATCY	6 cut(s)	715, 913, 1184, 1231, 2207, 2683
BstZ17I	GTATAC	1 cut(s)	3000
Bsu15I	ATCGAT	1 cut(s)	523
BsuI	GTATCC	1 cut(s)	2250
BsuRI	GGCC	7 cut(s)	5, 297, 1691, 2510, 2840, 3029, 3145
BsuTUI	ATCGAT	1 cut(s)	523
BtsCI	GGATG	8 cut(s)	287, 1180, 1957, 2583, 2623, 2894, 3494, 4012
BtsI	GCAGTG	1 cut(s)	268
BtsIMutI	CAGTG	9 cut(s)	268, 1226, 1651, 2411, 2650, 3137, 3916, 3971, 4080
BveI	ACCTGC	1 cut(s)	485
Cac8I	GCNNGC	5 cut(s)	541, 2838, 3379, 3492, 3845
CaiI	CAGNNNCTG	2 cut(s)	782, 3661
CfoI	GCGC	3 cut(s)	858, 2981, 3575
Cfr10I	RCCGGY	2 cut(s)	4024, 4107
Cfr13I	GGNCC	4 cut(s)	214, 2224, 2838, 2839
ClaI	ATCGAT	1 cut(s)	523
CseI	GACGC	2 cut(s)	1538, 2072
CspCI	CAANNNNNGTGG	2 cut(s)	2121, 2156
DraI	TTTAAA	3 cut(s)	684, 1362, 1986
EaeI	YGGCCR	3 cut(s)	3, 1689, 3027
Eam1104I	CTCTTC	3 cut(s)	2187, 2431, 3874
EarI	CTCTTC	3 cut(s)	2187, 2431, 3874
Ecl136II	GAGCTC	2 cut(s)	73, 407
Eco130I	CCWWGG	7 cut(s)	298, 1339, 1717, 2443, 3050, 3498, 4093
Eco147I	AGGCCT	1 cut(s)	297
Eco24I	GRGCYC	5 cut(s)	75, 409, 2842, 3225, 3464
Eco31I	GGTCTC	2 cut(s)	1829, 3282
Eco47I	GGWCC	2 cut(s)	214, 2224
Eco47III	AGCGCT	2 cut(s)	857, 2980
Eco53kI	GAGCTC	2 cut(s)	73, 407
Eco57I	CTGAAG	5 cut(s)	1038, 1107, 1401, 1821, 2892
Eco88I	CYCGRG	1 cut(s)	3111
Eco91I	GGTNACC	1 cut(s)	497
EcoICRI	GAGCTC	2 cut(s)	73, 407
EcoO109I	RGGNCCY	2 cut(s)	2224, 2839
EcoO65I	GGTNACC	1 cut(s)	497
EcoRI	GAATTC	1 cut(s)	3621
EcoRII	CCWGG	5 cut(s)	39, 379, 1513, 1960, 2842
EcoT14I	CCWWGG	7 cut(s)	298, 1339, 1717, 2443, 3050, 3498, 4093
EcoT38I	GRGCYC	5 cut(s)	75, 409, 2842, 3225, 3464
ErhI	CCWWGG	7 cut(s)	298, 1339, 1717, 2443, 3050, 3498, 4093
FaqI	GGGAC	5 cut(s)	844, 2210, 3032, 3521, 3995
FauI	CCCGC	3 cut(s)	2829, 3836, 4021
FauNDI	CATATG	1 cut(s)	1038
FblI	GTMKAC	2 cut(s)	1857, 2999
FokI	GGATG	8 cut(s)	274, 1187, 1944, 2590, 2610, 2881, 3481, 4019
FriOI	GRGCYC	5 cut(s)	75, 409, 2842, 3225, 3464
FspBI	CTAG	9 cut(s)	1077, 1182, 1340, 1376, 1578, 1923, 2681, 2717, 2783
GlaI	GCGC	3 cut(s)	857, 2980, 3574
GsuI	CTGGAG	6 cut(s)	1839, 2634, 3014, 3135, 3630, 3947
HaeII	RGCGCY	3 cut(s)	859, 2982, 3576
HaeIII	GGCC	7 cut(s)	5, 297, 1691, 2510, 2840, 3029, 3145
HapII	CCGG	6 cut(s)	674, 1793, 3357, 3668, 4025, 4108
HgaI	GACGC	2 cut(s)	1538, 2072
HhaI	GCGC	3 cut(s)	858, 2981, 3575
Hin6I	GCGC	3 cut(s)	856, 2979, 3573
HinP1I	GCGC	3 cut(s)	856, 2979, 3573
HincII	GTYRAC	1 cut(s)	55
HindII	GTYRAC	1 cut(s)	55
HindIII	AAGCTT	5 cut(s)	966, 1597, 1833, 3040, 3876
HinfI	GANTC	7 cut(s)	992, 1154, 1510, 2652, 2918, 3658, 3944
HpaI	GTTAAC	1 cut(s)	55
HpaII	CCGG	6 cut(s)	674, 1793, 3357, 3668, 4025, 4108
Hpy99I	CGWCG	1 cut(s)	11
HpyCH4IV	ACGT	3 cut(s)	1287, 3776, 3825
HpySE526I	ACGT	3 cut(s)	1287, 3776, 3825
HspAI	GCGC	3 cut(s)	856, 2979, 3573
Kpn2I	TCCGGA	1 cut(s)	673
KspAI	GTTAAC	1 cut(s)	55
LmnI	GCTCC	8 cut(s)	404, 757, 847, 935, 1892, 3360, 3451, 4036
LweI	GCATC	6 cut(s)	526, 1684, 2632, 2903, 3503, 3856
MaeI	CTAG	9 cut(s)	1077, 1182, 1340, 1376, 1578, 1923, 2681, 2717, 2783
MaeII	ACGT	3 cut(s)	1287, 3776, 3825
MaeIII	GTNAC	5 cut(s)	497, 943, 1288, 2255, 3133
MfeI	CAATTG	3 cut(s)	528, 939, 2430
MflI	RGATCY	6 cut(s)	715, 913, 1184, 1231, 2207, 2683
MlsI	TGGCCA	1 cut(s)	3029
MluNI	TGGCCA	1 cut(s)	3029
MlyI	GAGTC	2 cut(s)	1519, 3667
MmeI	TCCRAC	7 cut(s)	799, 941, 1182, 1261, 2467, 3178, 3984
Mox20I	TGGCCA	1 cut(s)	3029
MroI	TCCGGA	1 cut(s)	673
MscI	TGGCCA	1 cut(s)	3029
MslI	CAYNNNNRTG	3 cut(s)	654, 2525, 3013
Msp20I	TGGCCA	1 cut(s)	3029
MspA1I	CMGCKG	4 cut(s)	150, 557, 2282, 3843
MspCI	CTTAAG	1 cut(s)	2759
MspI	CCGG	6 cut(s)	674, 1793, 3357, 3668, 4025, 4108
MspR9I	CCNGG	7 cut(s)	41, 381, 1515, 1962, 2844, 3358, 3668
MunI	CAATTG	3 cut(s)	528, 939, 2430
MvaI	CCWGG	5 cut(s)	41, 381, 1515, 1962, 2844
NciI	CCSGG	2 cut(s)	3358, 3668
NdeI	CATATG	1 cut(s)	1038
NlaIV	GGNNCC	7 cut(s)	320, 717, 1233, 1715, 2226, 2840, 2841
NmuCI	GTSAC	2 cut(s)	2255, 3133
NspI	RCATGY	5 cut(s)	388, 1463, 2120, 2541, 2891
PaeR7I	CTCGAG	1 cut(s)	3111
PasI	CCCWGGG	1 cut(s)	2843
PceI	AGGCCT	1 cut(s)	297
PciI	ACATGT	1 cut(s)	2887
PcsI	WCGNNNNNNNCGW	1 cut(s)	2100
PfeI	GAWTC	5 cut(s)	992, 1154, 2652, 2918, 3944
PfoI	TCCNGGA	2 cut(s)	1960, 3356
PleI	GAGTC	2 cut(s)	1518, 3666
PpsI	GAGTC	2 cut(s)	1518, 3666
Ppu21I	YACGTR	1 cut(s)	3777
PpuMI	RGGWCCY	1 cut(s)	2224
PscI	ACATGT	1 cut(s)	2887
PshBI	ATTAAT	2 cut(s)	2696, 2934
PsiI	TTATAA	1 cut(s)	3786
Psp124BI	GAGCTC	2 cut(s)	75, 409
Psp1406I	AACGTT	2 cut(s)	1287, 3825
Psp5II	RGGWCCY	1 cut(s)	2224
Psp6I	CCWGG	5 cut(s)	39, 379, 1513, 1960, 2842
PspEI	GGTNACC	1 cut(s)	497
PspGI	CCWGG	5 cut(s)	39, 379, 1513, 1960, 2842
PspN4I	GGNNCC	7 cut(s)	320, 717, 1233, 1715, 2226, 2840, 2841
PspOMI	GGGCCC	1 cut(s)	2838
PspPI	GGNCC	4 cut(s)	214, 2224, 2838, 2839
PspPPI	RGGWCCY	1 cut(s)	2224
PstI	CTGCAG	1 cut(s)	2281
PstNI	CAGNNNCTG	2 cut(s)	782, 3661
PsuI	RGATCY	6 cut(s)	715, 913, 1184, 1231, 2207, 2683
PvuII	CAGCTG	3 cut(s)	150, 557, 2282
RseI	CAYNNNNRTG	3 cut(s)	654, 2525, 3013
SacI	GAGCTC	2 cut(s)	75, 409
Sau96I	GGNCC	4 cut(s)	214, 2224, 2838, 2839
SchI	GAGTC	2 cut(s)	1519, 3667
ScrFI	CCNGG	7 cut(s)	41, 381, 1515, 1962, 2844, 3358, 3668
SfaNI	GCATC	6 cut(s)	526, 1684, 2632, 2903, 3503, 3856
SfcI	CTRYAG	2 cut(s)	2277, 2907
Sfr274I	CTCGAG	1 cut(s)	3111
SinI	GGWCC	2 cut(s)	214, 2224
SlaI	CTCGAG	1 cut(s)	3111
SmiI	ATTTAAAT	1 cut(s)	1362
SmiMI	CAYNNNNRTG	3 cut(s)	654, 2525, 3013
SmlI	CTYRAG	4 cut(s)	2687, 2759, 3111, 3434
SmoI	CTYRAG	4 cut(s)	2687, 2759, 3111, 3434
SseBI	AGGCCT	1 cut(s)	297
SspI	AATATT	2 cut(s)	2746, 3409
SspMI	CTAG	9 cut(s)	1077, 1182, 1340, 1376, 1578, 1923, 2681, 2717, 2783
SstI	GAGCTC	2 cut(s)	75, 409
StuI	AGGCCT	1 cut(s)	297
StyD4I	CCNGG	7 cut(s)	39, 379, 1513, 1960, 2842, 3356, 3666
StyI	CCWWGG	7 cut(s)	298, 1339, 1717, 2443, 3050, 3498, 4093
SwaI	ATTTAAAT	1 cut(s)	1362
TaiI	ACGT	3 cut(s)	1290, 3779, 3828
TatI	WGTACW	5 cut(s)	374, 512, 665, 1243, 1907
TauI	GCSGC	2 cut(s)	156, 3975
TfiI	GAWTC	5 cut(s)	992, 1154, 2652, 2918, 3944
TscAI	CASTG	9 cut(s)	268, 1233, 1651, 2411, 2650, 3144, 3923, 3971, 4087
TseFI	GTSAC	2 cut(s)	2255, 3133
Tsp45I	GTSAC	2 cut(s)	2255, 3133
TspGWI	ACGGA	1 cut(s)	686
TspRI	CASTG	9 cut(s)	268, 1233, 1651, 2411, 2650, 3144, 3923, 3971, 4087
Vha464I	CTTAAG	1 cut(s)	2759
VpaK11BI	GGWCC	2 cut(s)	214, 2224
VspI	ATTAAT	2 cut(s)	2696, 2934
XapI	RAATTY	7 cut(s)	1090, 1488, 1939, 3071, 3105, 3621, 3789
XbaI	TCTAGA	2 cut(s)	1181, 2680
XceI	RCATGY	5 cut(s)	388, 1463, 2120, 2541, 2891
XcmI	CCANNNNNNNNNTGG	3 cut(s)	341, 1411, 1669
XhoI	CTCGAG	1 cut(s)	3111
XmaJI	CCTAGG	1 cut(s)	1339
XmiI	GTMKAC	2 cut(s)	1857, 2999
XspI	CTAG	9 cut(s)	1077, 1182, 1340, 1376, 1578, 1923, 2681, 2717, 2783

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.