Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Forward (+)

36516515 .. 36525000

Gene Structure 8486 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1162900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3756 bp

ATGCTTAAAGAGTACGACCCTGTTGTCGACGAAGTGAAAGATTTTACTGCAAGTTCATGTCTCACTCTGCTTTGTAGTATAAATGGATGTTCAATTACAACATCTGAAGGCCTTGGAAATATCAAAGACGGGTTCCACCCAATTCACCAAAGGATTGCTGGTTTCCATGCTTCTCAATGCGGCTTTTTGTACTCCTGGCATACGAATAGACCTGATCCCCCTCCTGGGTTCTCCAAGCTTTCTGTTTCCGAAGCTGAAAAGTCTATTGCAGGTAACCTCTGTCGATGTACTGGGTATCGACCGATTGCTGATGCCTGCAAGAGTTTTGCAGCTGATGTTGATATGGAGGATTTGGGGTTCAACTCCTTTTGGAAAAAGGGAGATAGTAAGGAAGTAAAAGTAAAGAGTTTGCCTCCATATAACCGTAATGGAGACATATGTACATTTCCGGACTTCTTAAAGAAAGAAATTCGTTCTTCCATGCCCTTGGATCCTAAGAGATATGGTTGGTACAATCCTGTTAGAGTTGAGGAGCTTCAAAACTTATTGAAAGATAATGATTTTGATAGTGCAAACGAGATGAAATTAGTTGTTGGAAACACAGGGACGGGCTATTACAAGGAACTAAAGCGCTATGACAGATACATTGATCTCAGATGTGTTCCTGAATTATCAATGATTAAGATCGATCCTACGGGGGCTGAGTTCGGAGCAATTGTAACAATCTCTAAAGTTATTGAGTCTTTGAGAAAGAATGACAACGGTGGATCTCCATCAAGAGGTGGTGATAGAAAATGTTTTCCTTCAGATATTGCTACAATACTTCTTGCTGTGGATTCAGAGGTAGATATAATGAATGGTGCTAGATCTGAGACGATTATGTTGGAGGATTTTCTGAGAAGGCCTCCACTGGATCCGAAAAGTGTACTTTTAAGTGTTAAAATCCCAAACTGGGAAGCAATTAGAAAAGGTTCTCGAGAAACCAACACCATGTTGCTTTTTGAAACCTATCGAGCTGCACCAAGACCCCTAGGAAGCGCATTGGCTTATTTGAATGCTGCCTTCCTAGCTGAAGTATCGTTTTGGAAGATTTCCAATGGAATTATGGTGGATCATTGTCGTCTGGCTTTTGGTGCTTATGGAACTAAACATGCAATTAGAGCAAGAAAGGTTGAGGAATTTTTAACTGGGAAAGTGCTGAGTCCTGGTGTTCTGTATGACGCTATTAAATTAGTTAGGGATGCTGTGGTTCCTGAAGAAGGAACTACGAGCCCTGCTTACAGGTCAAGCTTGGCTGCTGGTTTTATTTTCGAGTTCTTTAGCCCCTTAATTGACAGTGAAGGTTCCAATGGATTCTTGGGAAATACTTCGTTGGCCAATGCTTCTAAGTTAAAAAGGAACAAAAGCGCTAACGATAAGATGAGAACTGTTCTGTCATCTGGTAAACAGGTGATTGAACTAAGTACAGAGTATGATCCGGTTGGTAAGCCAATTACAAAATCTGGAGGTCTCATACAAGCTTCAGGTGAGGCAGTCTTTGTAGATGACATTCCATCTCCGAAATATTGCTTACATGGAGCGTTCATTTATAGTACAAAGCCTCTGGCTAGAGTGAAGGGAATAAAATTTGAACCTAAACCACATCCAGGAGTTGAACCTCTGTTTGCTGACGATCTTACCGAATGTGCTGGTCAGCGTCTTGCCTTCCTGGTAGCCGATACGCAGAAACATGCAGATATGGCTACAAACTTTGCGGTGGTTGATTATGGCATGGAAGATACAGATCCACCTATCTTGTCAGTCGAAGAGGCAGTTAAGAGAGCAAGTTTTTTTGAGGTCCCTCCTTTCTTGTATCCAAAACAGGTTGGTGACATATCAAATGGAATGGCTGCAGCTGATCACAAGATTATTTCTGCTGAGGTACTTGAAATCAATATGACATTCTTTCTTTGTTTCCCGATCAAACTTGGTTCACAATACTATTTCTACATGGAGACACAAACAGCCCTTGCTGTACCAGATGAAGAAAACTGCATGGTGGTTTACACTTCAAGTCAAGGGCCTGAGTATGCCCACTCTACAATTGCAAAATGTCTTGGTATTCCTGAAAATAATATTCGTGTAATTACAAGAAGGGTCGGAGGGGGCTTTGGTGGAAAGGGCATACAATCAGTGCCTGTGAGTCGCAACTGCATGTGCTCTTGCAGCACACACAAATTACATCATCGACCAGTGCGGATGTATCTCAATCGCAAGACTGATATGATAATGGCTGGAGGAAGGCATCCCATGAAGATAACTTACAGTGTGGGATTCAAGTCTGATGGGAAGATTACAGCTTTAGAACTTGAGATATTGATAAATGCTGGGATTTCCATAGGCTTTAGTCCAGTATTGCCAAGAACTATTCTGTGGGCACTTAAGAAGTATGACTGGGGTGCTCTGGCTTTCGATATAAAGGTGTGCAAAACAAACACTCCGAGTAGGTCTACTATGCGGGCCCCTGGGGAGGTGCAGGGGTCATTTATCGCTGAAGCTGTAATTGAACATGTAGCATCCACCCTTTCTATAGAAGTAGATTATGTGAGAAGTGTTAATCTTCACACATCTCGCAGCCTTGATTTGTTTTACGAGCACAGCGCTGGTGAACCTCTGGAGTATACTTTACCATTGATATGGAACAAGTTGGCCATGTCTTCAAGCTTTAACCCAAGGACTGAGATGGTAAAAGAATTTAATAGGTGTACTAAATGGCAGAAAAGAGGAATTTCTCGAGTACCGATATTGTGTGAAGTGTCTTTGAGGCCAACTCCGGGAAAAGTAAGCATTTTAAGTGATGGGTCTGTTGTTGTTGAAGTTGGAGGAATTGAACTGGGTCAGGGGCTCTGGACGAAGGTAAAACAGGTGACAGCTTTTGCTCTTGGTTCGATTCAATGCGATGGTAGTGGAGATCTTTCGGATAAAGTACGGGTGGTGCAATCAGATACGTTGAGCTTAATTCAAGGGGGAATTACTGTCGGGAGCACTACATCTGAGGCAAGCTGTGAGGCAGTTAGACTTTGCTGTAATATTTTGGTTGAGAGGTTAGCTACTCTCAAAGGGAGATTGAAGGAGCAAATGGGCTCTATTAACTGGGAGACTCGTATTCAGCAGGCATCCTTGCAAGATGTGAACTTATCAGCAAGTTCTTATTATGTCCCTGATTTTGCTTCCAAGAGATACCTTAACTATGGCGCTGCTGTGAGTGAGGTGGAGGTAAATGTTCTGACTGGACAAACCAGAATTCTGCAATCGGATATTATCTACGATTGTGGTCAGAGTCTCAACCCTGCTGTGGATTTAGGACAGATCGAAGGTGCTTTTGTTCAAGGTATTGGGTTTTTCATGCTTGAAGAATACTCGGAAAACTCTGATGGGTTGGTGATTTCAGAAGGTACATGGACGTATAAGATTCCTACAATGGACACCATACCCAAACGGTTCAACGTTGAAGTATTGAGCAGTGGGCATCACAAAAAACGCGTGCTCTCTTCAAAAGCTTCTGGTGAGCCACCATTACTTCTTGCGGTTTCTGTTCACTGTGCGACAAGGGCTGCCATTAAAGAATCAAGAAAACAGCTTCTTGAGTGGGGCGGCCTAGATGGATCGGCATCAATATTCCAGTTGGATGTTCCCGCTACCATGCCTGTTGTGAAGGAGCTGTGTGGGCTGGAAGCTGTGGAGAGGTACTTGGAATGGATTGTAGGCAGAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1252

Amino Acids

137.57

Weight (kDa)

6.54

Isoelectric Point (pI)

37.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2_2	PF01799	34 - 107	3.1e-10	[2Fe-2S] binding domain
FAD_binding_5	PF00941	167 - 251	3.1e-13	FAD binding domain in molybdopterin dehydrogenase
FAD_binding_5	PF00941	268 - 318	1e-07	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	336 - 441	1.5e-19	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	508 - 545	8.2e-07	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
Ald_Xan_dh_C	PF01315	552 - 590	4.5e-06	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	654 - 867	2.8e-69	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	897 - 1158	9.3e-69	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	23
Acc36I	ACCTGC	1 cut(s)	260
AccI	GTMKAC	3 cut(s)	27, 2489, 2660
AccII	CGCG	1 cut(s)	3522
AccIII	TCCGGA	1 cut(s)	448
AciI	CCGC	7 cut(s)	180, 1754, 2236, 2497, 3566, 3633, 3675
AclI	AACGTT	1 cut(s)	3486
AcoI	YGGCCR	2 cut(s)	1374, 2688
AcsI	RAATTY	6 cut(s)	468, 1178, 1625, 2732, 2766, 3282
AcuI	CTGAAG	6 cut(s)	126, 789, 1092, 1275, 1506, 2553
AfeI	AGCGCT	3 cut(s)	632, 1408, 2641
AfiI	CCNNNNNNNGG	9 cut(s)	224, 225, 779, 952, 1259, 1646, 2509, 2813, 3334
AflII	CTTAAG	1 cut(s)	2420
AflIII	ACRYGT	2 cut(s)	2548, 3520
AjnI	CCWGG	6 cut(s)	194, 223, 1204, 1645, 1707, 2503
AloI	GAACNNNNNNTCC	2 cut(s)	341, 373
Alw21I	GWGCWC	5 cut(s)	2201, 2443, 2637, 3026, 3528
Alw26I	GTCTC	7 cut(s)	65, 426, 866, 1514, 1988, 3131, 3326
AlwNI	CAGNNNCTG	1 cut(s)	2177
Ama87I	CYCGRG	2 cut(s)	975, 2772
Aor13HI	TCCGGA	1 cut(s)	448
Aor51HI	AGCGCT	3 cut(s)	632, 1408, 2641
AoxI	GGCC	8 cut(s)	109, 902, 1374, 2060, 2499, 2688, 2804, 3634
ApaI	GGGCCC	1 cut(s)	2503
ApoI	RAATTY	6 cut(s)	468, 1178, 1625, 2732, 2766, 3282
Asp700I	GAANNNNTTC	2 cut(s)	970, 1366
AspA2I	CCTAGG	1 cut(s)	1030
AspLEI	GCGC	5 cut(s)	633, 1040, 1409, 2642, 3236
AspS9I	GGNCC	4 cut(s)	1837, 2060, 2499, 2500
AsuC2I	CCSGG	1 cut(s)	2814
AsuHPI	GGTGA	9 cut(s)	137, 797, 1462, 1538, 1880, 2657, 2917, 3433, 3557
AvaI	CYCGRG	2 cut(s)	975, 2772
AvaII	GGWCC	1 cut(s)	1837
AvrII	CCTAGG	1 cut(s)	1030
BaeGI	GKGCMC	2 cut(s)	2419, 2503
BalI	TGGCCA	2 cut(s)	1376, 2690
BamHI	GGATCC	2 cut(s)	490, 913
BanII	GRGCYC	4 cut(s)	1274, 2503, 2886, 3125
BarI	GAAGNNNNNNTAC	2 cut(s)	2285, 2317
BbsI	GAAGAC	1 cut(s)	2688
Bbv12I	GWGCWC	5 cut(s)	2201, 2443, 2637, 3026, 3528
BbvCI	CCTCAGC	1 cut(s)	1917
BccI	CCATC	8 cut(s)	781, 1561, 2318, 2716, 2831, 2933, 3407, 3635
BciT130I	CCWGG	6 cut(s)	196, 225, 1206, 1647, 1709, 2505
BciVI	GTATCC	1 cut(s)	1863
BclI	TGATCA	1 cut(s)	1897
BcnI	CCSGG	1 cut(s)	2814
BcoDI	GTCTC	7 cut(s)	65, 426, 866, 1514, 1988, 3131, 3326
BfaI	CTAG	5 cut(s)	864, 1031, 1067, 1608, 3638
BfmI	CTRYAG	2 cut(s)	1890, 2568
BfoI	RGCGCY	4 cut(s)	634, 1410, 2643, 3237
BfrI	CTTAAG	1 cut(s)	2420
BfuAI	ACCTGC	1 cut(s)	260
BfuI	GTATCC	1 cut(s)	1863
BglII	AGATCT	2 cut(s)	866, 2950
BlnI	CCTAGG	1 cut(s)	1030
Bme1390I	CCNGG	7 cut(s)	196, 225, 1206, 1647, 1709, 2505, 2814
Bme18I	GGWCC	1 cut(s)	1837
BmeT110I	CYCGRG	2 cut(s)	975, 2772
BmgT120I	GGNCC	4 cut(s)	1837, 2060, 2499, 2500
BmiI	GGNNCC	8 cut(s)	134, 492, 915, 1251, 1345, 1839, 2501, 2502
BmrFI	CCNGG	7 cut(s)	196, 225, 1206, 1647, 1709, 2505, 2814
BmrI	ACTGGG	6 cut(s)	300, 961, 1197, 2443, 2882, 3142
BmsI	GCATC	7 cut(s)	301, 1231, 2293, 2564, 3164, 3517, 3659
BmuI	ACTGGG	6 cut(s)	300, 961, 1197, 2443, 2882, 3142
BpiI	GAAGAC	1 cut(s)	2688
BplI	GAGNNNNNCTC	6 cut(s)	397, 429, 889, 921, 2794, 2826
BpmI	CTGGAG	3 cut(s)	1524, 2295, 2675
Bpu10I	CCTNAGC	1 cut(s)	1917
BpuEI	CTTGAG	2 cut(s)	2369, 3644
BpuMI	CCSGG	1 cut(s)	2814
Bsa29I	ATCGAT	1 cut(s)	687
BsaBI	GATNNNNATC	4 cut(s)	683, 772, 1782, 3649
BsaI	GGTCTC	1 cut(s)	1514
BsaJI	CCNNGG	7 cut(s)	112, 224, 486, 1030, 2503, 2504, 2711
BsaWI	WCCGGW	2 cut(s)	448, 1477
BsaXI	ACNNNNNCTCC	4 cut(s)	702, 732, 2461, 2491
Bsc4I	CCNNNNNNNGG	9 cut(s)	224, 225, 779, 952, 1259, 1646, 2509, 2813, 3334
Bse8I	GATNNNNATC	4 cut(s)	683, 772, 1782, 3649
BseAI	TCCGGA	1 cut(s)	448
BseBI	CCWGG	6 cut(s)	196, 225, 1206, 1647, 1709, 2505
BseCI	ATCGAT	1 cut(s)	687
BseDI	CCNNGG	7 cut(s)	112, 224, 486, 1030, 2503, 2504, 2711
BseGI	GGATG	8 cut(s)	92, 1246, 1642, 2244, 2284, 2555, 3155, 3673
BseJI	GATNNNNATC	4 cut(s)	683, 772, 1782, 3649
BseLI	CCNNNNNNNGG	9 cut(s)	224, 225, 779, 952, 1259, 1646, 2509, 2813, 3334
BseMII	CTCAG	9 cut(s)	667, 693, 861, 887, 1190, 1908, 2055, 2709, 3024
BseRI	GAGGAG	1 cut(s)	545
BseSI	GKGCMC	2 cut(s)	2419, 2503
BseYI	CCCAGC	1 cut(s)	2366
BsgI	GTGCAG	2 cut(s)	1002, 2534
Bsh1236I	CGCG	1 cut(s)	3522
Bsh1285I	CGRYCG	1 cut(s)	302
BshFI	GGCC	8 cut(s)	111, 904, 1376, 2062, 2501, 2690, 2806, 3636
BshVI	ATCGAT	1 cut(s)	687
BsiEI	CGRYCG	1 cut(s)	302
BsiHKAI	GWGCWC	5 cut(s)	2201, 2443, 2637, 3026, 3528
BsiHKCI	CYCGRG	2 cut(s)	975, 2772
BsiSI	CCGG	3 cut(s)	449, 1478, 2813
BslFI	GGGAC	3 cut(s)	619, 1823, 3182
BslI	CCNNNNNNNGG	9 cut(s)	224, 225, 779, 952, 1259, 1646, 2509, 2813, 3334
BsmAI	GTCTC	7 cut(s)	65, 426, 866, 1514, 1988, 3131, 3326
BsmBI	CGTCTC	1 cut(s)	866
BsmFI	GGGAC	3 cut(s)	619, 1823, 3182
BsmI	GAATGC	1 cut(s)	1060
BsnI	GGCC	8 cut(s)	111, 904, 1376, 2062, 2501, 2690, 2806, 3636
Bso31I	GGTCTC	1 cut(s)	1514
BsoBI	CYCGRG	2 cut(s)	975, 2772
Bsp120I	GGGCCC	1 cut(s)	2499
Bsp13I	TCCGGA	1 cut(s)	448
Bsp1407I	TGTACA	1 cut(s)	440
BspACI	CCGC	7 cut(s)	180, 1754, 2236, 2497, 3566, 3633, 3675
BspANI	GGCC	8 cut(s)	111, 904, 1376, 2062, 2501, 2690, 2806, 3636
BspCNI	CTCAG	9 cut(s)	666, 694, 862, 888, 1191, 1909, 2056, 2710, 3025
BspDI	ATCGAT	1 cut(s)	687
BspEI	TCCGGA	1 cut(s)	448
BspFNI	CGCG	1 cut(s)	3522
BspLI	GGNNCC	8 cut(s)	134, 492, 915, 1251, 1345, 1839, 2501, 2502
BspMAI	CTGCAG	1 cut(s)	1894
BspMI	ACCTGC	1 cut(s)	260
BspTI	CTTAAG	1 cut(s)	2420
BspTNI	GGTCTC	1 cut(s)	1514
BsrGI	TGTACA	1 cut(s)	440
BssECI	CCNNGG	7 cut(s)	112, 224, 486, 1030, 2503, 2504, 2711
BssNAI	GTATAC	1 cut(s)	2661
BssT1I	CCWWGG	4 cut(s)	112, 486, 1030, 2711
Bst1107I	GTATAC	1 cut(s)	2661
Bst2UI	CCWGG	6 cut(s)	196, 225, 1206, 1647, 1709, 2505
Bst4CI	ACNGT	8 cut(s)	425, 764, 1337, 1429, 2306, 3016, 3480, 3581
Bst6I	CTCTTC	2 cut(s)	1800, 3535
BstAFI	CTTAAG	1 cut(s)	2420
BstAUI	TGTACA	1 cut(s)	440
BstC8I	GCNNGC	5 cut(s)	316, 2499, 3040, 3153, 3524
BstEII	GGTNACC	1 cut(s)	272
BstENI	CCTNNNNNAGG	1 cut(s)	1257
BstF5I	GGATG	8 cut(s)	92, 1246, 1642, 2244, 2284, 2555, 3155, 3673
BstFNI	CGCG	1 cut(s)	3522
BstH2I	RGCGCY	4 cut(s)	634, 1410, 2643, 3237
BstHHI	GCGC	5 cut(s)	633, 1040, 1409, 2642, 3236
BstMAI	GTCTC	7 cut(s)	65, 426, 866, 1514, 1988, 3131, 3326
BstMCI	CGRYCG	1 cut(s)	302
BstMWI	GCNNNNNNNGC	7 cut(s)	1067, 1133, 1160, 1739, 2205, 3590, 3706
BstNI	CCWGG	6 cut(s)	196, 225, 1206, 1647, 1709, 2505
BstNSI	RCATGY	4 cut(s)	1154, 1733, 2197, 2552
BstPI	GGTNACC	1 cut(s)	272
BstSCI	CCNGG	7 cut(s)	194, 223, 1204, 1645, 1707, 2503, 2812
BstSFI	CTRYAG	2 cut(s)	1890, 2568
BstSLI	GKGCMC	2 cut(s)	2419, 2503
BstUI	CGCG	1 cut(s)	3522
BstV2I	GAAGAC	1 cut(s)	2688
BstX2I	RGATCY	6 cut(s)	490, 767, 866, 913, 1783, 2950
BstXI	CCANNNNNNTGG	2 cut(s)	487, 2676
BstYI	RGATCY	6 cut(s)	490, 767, 866, 913, 1783, 2950
BstZ17I	GTATAC	1 cut(s)	2661
Bsu15I	ATCGAT	1 cut(s)	687
BsuI	GTATCC	1 cut(s)	1863
BsuRI	GGCC	8 cut(s)	111, 904, 1376, 2062, 2501, 2690, 2806, 3636
BsuTUI	ATCGAT	1 cut(s)	687
BtgZI	GCGATG	1 cut(s)	2952
BtsCI	GGATG	8 cut(s)	92, 1246, 1642, 2244, 2284, 2555, 3155, 3673
BtsI	GCAGTG	1 cut(s)	3508
BtsIMutI	CAGTG	7 cut(s)	908, 1342, 2178, 2238, 2311, 3508, 3577
BveI	ACCTGC	1 cut(s)	260
Cac8I	GCNNGC	5 cut(s)	316, 2499, 3040, 3153, 3524
CaiI	CAGNNNCTG	1 cut(s)	2177
CfoI	GCGC	5 cut(s)	633, 1040, 1409, 2642, 3236
Cfr13I	GGNCC	4 cut(s)	1837, 2060, 2499, 2500
ClaI	ATCGAT	1 cut(s)	687
CseI	GACGC	2 cut(s)	1229, 1685
CspCI	CAANNNNNGTGG	2 cut(s)	1776, 1811
DrdI	GACNNNNNNGTC	1 cut(s)	23
DseDI	GACNNNNNNGTC	1 cut(s)	23
EaeI	YGGCCR	2 cut(s)	1374, 2688
Eam1104I	CTCTTC	2 cut(s)	1800, 3535
EarI	CTCTTC	2 cut(s)	1800, 3535
Eco130I	CCWWGG	4 cut(s)	112, 486, 1030, 2711
Eco147I	AGGCCT	2 cut(s)	111, 904
Eco24I	GRGCYC	4 cut(s)	1274, 2503, 2886, 3125
Eco31I	GGTCTC	1 cut(s)	1514
Eco47I	GGWCC	1 cut(s)	1837
Eco47III	AGCGCT	3 cut(s)	632, 1408, 2641
Eco57I	CTGAAG	6 cut(s)	126, 789, 1092, 1275, 1506, 2553
Eco88I	CYCGRG	2 cut(s)	975, 2772
Eco91I	GGTNACC	1 cut(s)	272
EcoNI	CCTNNNNNAGG	1 cut(s)	1257
EcoO109I	RGGNCCY	3 cut(s)	1837, 2060, 2500
EcoO65I	GGTNACC	1 cut(s)	272
EcoRI	GAATTC	1 cut(s)	3282
EcoRII	CCWGG	6 cut(s)	194, 223, 1204, 1645, 1707, 2503
EcoT14I	CCWWGG	4 cut(s)	112, 486, 1030, 2711
EcoT38I	GRGCYC	4 cut(s)	1274, 2503, 2886, 3125
ErhI	CCWWGG	4 cut(s)	112, 486, 1030, 2711
Esp3I	CGTCTC	1 cut(s)	866
FaqI	GGGAC	3 cut(s)	619, 1823, 3182
FauI	CCCGC	2 cut(s)	2490, 3682
FauNDI	CATATG	1 cut(s)	437
FbaI	TGATCA	1 cut(s)	1897
FblI	GTMKAC	3 cut(s)	27, 2489, 2660
FokI	GGATG	8 cut(s)	99, 1253, 1629, 2251, 2271, 2542, 3142, 3680
FriOI	GRGCYC	4 cut(s)	1274, 2503, 2886, 3125
FspBI	CTAG	5 cut(s)	864, 1031, 1067, 1608, 3638
GlaI	GCGC	5 cut(s)	632, 1039, 1408, 2641, 3235
GsaI	CCCAGC	1 cut(s)	2370
GsuI	CTGGAG	3 cut(s)	1524, 2295, 2675
HaeII	RGCGCY	4 cut(s)	634, 1410, 2643, 3237
HaeIII	GGCC	8 cut(s)	111, 904, 1376, 2062, 2501, 2690, 2806, 3636
HapII	CCGG	3 cut(s)	449, 1478, 2813
HgaI	GACGC	2 cut(s)	1229, 1685
HhaI	GCGC	5 cut(s)	633, 1040, 1409, 2642, 3236
Hin6I	GCGC	5 cut(s)	631, 1038, 1407, 2640, 3234
HinP1I	GCGC	5 cut(s)	631, 1038, 1407, 2640, 3234
HincII	GTYRAC	1 cut(s)	28
HindII	GTYRAC	1 cut(s)	28
HindIII	AAGCTT	5 cut(s)	236, 1288, 1518, 2701, 3537
HpaII	CCGG	3 cut(s)	449, 1478, 2813
HphI	GGTGA	9 cut(s)	137, 797, 1462, 1538, 1880, 2657, 2917, 3433, 3557
Hpy99I	CGWCG	1 cut(s)	32
HpyCH4III	ACNGT	8 cut(s)	425, 764, 1337, 1429, 2306, 3016, 3480, 3581
HpyCH4IV	ACGT	3 cut(s)	2987, 3443, 3486
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1067, 1133, 1160, 1739, 2205, 3590, 3706
HpySE526I	ACGT	3 cut(s)	2987, 3443, 3486
HspAI	GCGC	5 cut(s)	631, 1038, 1407, 2640, 3234
Kpn2I	TCCGGA	1 cut(s)	448
Ksp22I	TGATCA	1 cut(s)	1897
LmnI	GCTCC	6 cut(s)	532, 710, 1577, 3021, 3112, 3697
LweI	GCATC	7 cut(s)	301, 1231, 2293, 2564, 3164, 3517, 3659
MaeI	CTAG	5 cut(s)	864, 1031, 1067, 1608, 3638
MaeII	ACGT	3 cut(s)	2987, 3443, 3486
MaeIII	GTNAC	4 cut(s)	272, 718, 1868, 2905
MfeI	CAATTG	2 cut(s)	714, 2082
MflI	RGATCY	6 cut(s)	490, 767, 866, 913, 1783, 2950
MlsI	TGGCCA	2 cut(s)	1376, 2690
MluI	ACGCGT	1 cut(s)	3520
MluNI	TGGCCA	2 cut(s)	1376, 2690
MlyI	GAGTC	5 cut(s)	749, 1210, 2191, 3133, 3328
MmeI	TCCRAC	5 cut(s)	574, 864, 2119, 2839, 3645
Mox20I	TGGCCA	2 cut(s)	1376, 2690
MroI	TCCGGA	1 cut(s)	448
MroXI	GAANNNNTTC	2 cut(s)	970, 1366
MscI	TGGCCA	2 cut(s)	1376, 2690
MslI	CAYNNNNRTG	1 cut(s)	2674
Msp20I	TGGCCA	2 cut(s)	1376, 2690
MspA1I	CMGCKG	2 cut(s)	332, 1895
MspCI	CTTAAG	1 cut(s)	2420
MspI	CCGG	3 cut(s)	449, 1478, 2813
MspR9I	CCNGG	7 cut(s)	196, 225, 1206, 1647, 1709, 2505, 2814
MunI	CAATTG	2 cut(s)	714, 2082
Mva1269I	GAATGC	1 cut(s)	1060
MvaI	CCWGG	6 cut(s)	196, 225, 1206, 1647, 1709, 2505
MvnI	CGCG	1 cut(s)	3522
MwoI	GCNNNNNNNGC	7 cut(s)	1067, 1133, 1160, 1739, 2205, 3590, 3706
NciI	CCSGG	1 cut(s)	2814
NdeI	CATATG	1 cut(s)	437
NlaIV	GGNNCC	8 cut(s)	134, 492, 915, 1251, 1345, 1839, 2501, 2502
NmuCI	GTSAC	2 cut(s)	1868, 2905
NspI	RCATGY	4 cut(s)	1154, 1733, 2197, 2552
PaeR7I	CTCGAG	2 cut(s)	975, 2772
PasI	CCCWGGG	1 cut(s)	2504
PceI	AGGCCT	2 cut(s)	111, 904
PciI	ACATGT	1 cut(s)	2548
PcsI	WCGNNNNNNNCGW	1 cut(s)	1677
PctI	GAATGC	1 cut(s)	1060
PdmI	GAANNNNTTC	2 cut(s)	970, 1366
PfeI	GAWTC	6 cut(s)	836, 1353, 2313, 2929, 3451, 3605
PfoI	TCCNGGA	2 cut(s)	1645, 2812
PleI	GAGTC	5 cut(s)	748, 1209, 2190, 3133, 3327
PpsI	GAGTC	5 cut(s)	748, 1209, 2190, 3133, 3327
PpuMI	RGGWCCY	1 cut(s)	1837
PscI	ACATGT	1 cut(s)	2548
Psp1406I	AACGTT	1 cut(s)	3486
Psp5II	RGGWCCY	1 cut(s)	1837
Psp6I	CCWGG	6 cut(s)	194, 223, 1204, 1645, 1707, 2503
PspEI	GGTNACC	1 cut(s)	272
PspFI	CCCAGC	1 cut(s)	2366
PspGI	CCWGG	6 cut(s)	194, 223, 1204, 1645, 1707, 2503
PspN4I	GGNNCC	8 cut(s)	134, 492, 915, 1251, 1345, 1839, 2501, 2502
PspOMI	GGGCCC	1 cut(s)	2499
PspPI	GGNCC	4 cut(s)	1837, 2060, 2499, 2500
PspPPI	RGGWCCY	1 cut(s)	1837
PstI	CTGCAG	1 cut(s)	1894
PstNI	CAGNNNCTG	1 cut(s)	2177
PsuI	RGATCY	6 cut(s)	490, 767, 866, 913, 1783, 2950
PvuII	CAGCTG	2 cut(s)	332, 1895
RseI	CAYNNNNRTG	1 cut(s)	2674
SalI	GTCGAC	1 cut(s)	26
Sau96I	GGNCC	4 cut(s)	1837, 2060, 2499, 2500
SchI	GAGTC	5 cut(s)	749, 1210, 2191, 3133, 3328
ScrFI	CCNGG	7 cut(s)	196, 225, 1206, 1647, 1709, 2505, 2814
SfaNI	GCATC	7 cut(s)	301, 1231, 2293, 2564, 3164, 3517, 3659
SfcI	CTRYAG	2 cut(s)	1890, 2568
Sfr274I	CTCGAG	2 cut(s)	975, 2772
SinI	GGWCC	1 cut(s)	1837
SlaI	CTCGAG	2 cut(s)	975, 2772
SmiMI	CAYNNNNRTG	1 cut(s)	2674
SmlI	CTYRAG	5 cut(s)	975, 2348, 2420, 2772, 3623
SmoI	CTYRAG	5 cut(s)	975, 2348, 2420, 2772, 3623
SseBI	AGGCCT	2 cut(s)	111, 904
SsiI	CCGC	7 cut(s)	180, 1754, 2236, 2497, 3566, 3633, 3675
SspI	AATATT	4 cut(s)	1565, 2116, 3070, 3657
SspMI	CTAG	5 cut(s)	864, 1031, 1067, 1608, 3638
StuI	AGGCCT	2 cut(s)	111, 904
StyD4I	CCNGG	7 cut(s)	194, 223, 1204, 1645, 1707, 2503, 2812
StyI	CCWWGG	4 cut(s)	112, 486, 1030, 2711
TaaI	ACNGT	8 cut(s)	425, 764, 1337, 1429, 2306, 3016, 3480, 3581
TaiI	ACGT	3 cut(s)	2990, 3446, 3489
TaqII	GACCGA	1 cut(s)	316
TatI	WGTACW	7 cut(s)	189, 287, 440, 925, 1463, 1592, 2744
TauI	GCSGC	2 cut(s)	183, 3636
TfiI	GAWTC	6 cut(s)	836, 1353, 2313, 2929, 3451, 3605
TscAI	CASTG	7 cut(s)	915, 1342, 2178, 2238, 2311, 3508, 3584
TseFI	GTSAC	2 cut(s)	1868, 2905
Tsp45I	GTSAC	2 cut(s)	1868, 2905
TspDTI	ATGAA	7 cut(s)	45, 596, 869, 1573, 2037, 2306, 3373
TspRI	CASTG	7 cut(s)	915, 1342, 2178, 2238, 2311, 3508, 3584
Vha464I	CTTAAG	1 cut(s)	2420
VpaK11BI	GGWCC	1 cut(s)	1837
XagI	CCTNNNNNAGG	1 cut(s)	1257
XapI	RAATTY	6 cut(s)	468, 1178, 1625, 2732, 2766, 3282
XceI	RCATGY	4 cut(s)	1154, 1733, 2197, 2552
XcmI	CCANNNNNNNNNTGG	3 cut(s)	155, 1102, 1354
XhoI	CTCGAG	2 cut(s)	975, 2772
XmaJI	CCTAGG	1 cut(s)	1030
XmiI	GTMKAC	3 cut(s)	27, 2489, 2660
XmnI	GAANNNNTTC	2 cut(s)	970, 1366
XspI	CTAG	5 cut(s)	864, 1031, 1067, 1608, 3638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.