Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Reverse (-)

48760968 .. 48770892

Gene Structure 9925 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG325300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3906 bp

ATGGCCGGAACTAGCTTGGTCTTCGCTGTTAATCGCCAGAGGTTTCACCTCCCTACTGTCGATCCTTCCACCACTCTGCTCGAGTTTCTCCGTTCCCACACTCGCTTCAAGAGCGTCAAGCTCTCCTGTGGCGAAGGTGGTTGTGGTGCTTGTGTTGTGCTACTGTCCAAGTATGATCCTGTTCTTGACAGAGTGGAGGATTTTGCTGTTAGTTCATGTCTCACTCTACTCTGCAGCGTAAACCTATGCTCTATTACAACCTCTGAAGGGTTTGGGAATAGTAAAGATGGTTTTCATGCAATTCACCAGAGGTTCAGCGGTTTCCACGCTTCTCAATGCGGGTTTTGTACTCCCGGGATGTGTGTTTCCCTCTTTTCGGCTCTTGTCAATGCTGAAAAGAAGGCCGATCGAGTGGAGGCCCCTCCCGGGTTCTCCAAGCTGACTGTGTCTGAAGCTGAGAAGGCTGTTGCAGGAAACCTCTGTCGCTGTACTGGGTATCGTTCCATCGCTGATGCCTGCAAGAGCTTTTCGGCTGATGTTGACATGGAGGATTTGGGGTTCAACTCCTTCTGGAGGAAGGGAGACAGTAAGCAAGCGAAGATAGATAGTTTGCCTCCTTATAATCCTGAGAGTGAGGTTTGTACATTCCCTGAGTTTTTGAAAAATGAAATCAGGTCCTTGTCGTCGTTTTTGGATTCCAAGAGATATGGTTGGTACAGTCCAGCTACTATTGAGGAGCTGAAGAGCTTAGTGGATGCAAATGATTCTGGTGATAGTATGAAGTTGGTTGTTGGTAACACAGGGACGGGGTATTACAAGGAGTTAACAGGTTATGACAAATACATTGATCTCAGATATGTTCCTGAACTATCAATAATTAGGATGGATCCGACGGGTCTTGATGTAGGAGCAATTGTAACCATCACTAAAGTTATTGAAGCTTTAAAGAAGAAAACCAAAGGTGAACTTATCTCAAGAGGTGAGCTAGTGTTTGAAAGAATTGCCAGGCATATGGATAAAATTGCTTCAGGGTTTCTCAGAAATACAGCTAGTATAGGAGGGAATTTGGTGATGGCACAAAGAAAGAACTTTCCTTCAGATATTGCTACAATACTTCTCGCTGTGGATTCAACAGTGAATATCATGAGTGGTTCTAGATCTGAAATTATTATGTTGGAGGATTTTCTGAAAAGAGCCCCATTGGATCCCAAAAGTGTACTTTTAAGTGTTAAAATCCCAAACTGGGAAGCAGTTACAAAAGTTTCTGCAGGATCCAATACTATGTTGCTATTTGAAACCTATAGAGCTGCACCCAGACCGCTAGGAAATGCATTGCCTTATTTGAATGCTGCTTTCTTGGCTGAAGTTTCTTCATGTAAAACTTCTACTGGATTTATGGTACATCACTGCTGTCTGGCTTTTGGTGCTTATGGGACTAAACATGCAATTAGAGCAAGAAAGGTTGAGGACTTTTTAAACGGAAAAACCTTGAGTGCTGGTGTTTTGTATGAAGCGATTAAGTTAGTTCGAGCTACTGTGGTTCCTGAAGAAGGCACTACGAGTCCTGCTTACAGGTCAAGCTTGGCTTGTGGTTTTCTTTTTGAGTTCTTTAGCCCCTTTATTAACAGTGACACCGAAATTTCTGGTGGTTTCTTGGAGAATATATTGTTCCCTACTTCTAAGATGAATAAGAACCAATGTTGCAATGATGAGATTCCATATGTTTCATCATCAGCTAAACAAGTGCTTAATTTAAGTGCAGACTATGATCCAGTCGGCAAGCCAATTATAAAATCTGGAGCTGCTATCCAAGCTTCAGGTGAGGCTGTGTATGTAGATGACATTCCATCACCAACAAATTGCTTGCATGGAGCGTTCATTTATAGCACAAAGCCTTTCGTACGGGTTAAGGGAATAAAATTCAAAACTAAACCACAGCCAGATGGAGTTTCTGCTGTCTTATCTTTTAAAGATATCCCCAATGGTGGGGAGAATGTCGGATCTAAGACTATTTTTGGTACTGAACCTCTTTTTGCTGATGATCTTACTCAATGTGCTGGTCAGCGTCTTGCCTTTTTGGTTGCAGATACACAGAAAAATGCAGATTTGGCTGCAAATTTTGTGGATGTTGATTATGAAATGGAAACTATAGAACAACCTATACTATCCGTAGAGGAGGCTGTTAAGAGGTCTAGTTTTTTTGAGGTCCCTCCTTTCTTATACCCGAAGCAAGTTGGTGACATATCAAAAGGAATGGCTTTAGCTGATCACAAGATTACATCTGCTGAGATCAAACTTGGTTCACAATACTATTTCTACATGGAGACACAAACTGCCCTTGCTGTACCAGATGAAGACAACTGCATAGTGGTTTATACTTCAAGTCAGTGTCCTCAGTTTCAACACTCAGTCATCGCAAAGTGTCTTGGCATTCCTGAAAGTAATGTCCGTGTAATTACAAGGAGGGTTGGTGGGGGTTTTGGTGGAAAGGCCGTAAAATCCATTCCTGTCGCAACTGCATGTGCACTAGCGGCACACAAATTACACCGCCCTGTCAGGATATATGTCAATCGCAAGACTGATATGATAATGGCAGGAGGAAGGCATCCCATGAAGATAATTTACAGTGTGGGATTCAAGTCTGATGGGAAGATTACAGCTTTACAACTTGATATATTAATCGATGCAGGGATGGCCCTAGATATAAGTCCAATTATGCCATACAATGTTCTGGGTGCACTTAAGAAGTATGATTGGGGTGCTCTGTCTTTTGATGTAAAGGTATGCAAAACTAACAATCCAAGTAGGTCTGCTATGCGGGGTCCTGGGGAGGTGCAGGGTTCATTTATTGCTGAAGCTGTAATTGAACATGTAGCATCTACCCTTTCCATGGAAGTGGATTGTGTGAGAAATATAAATCTTCACACACATTGCAGCCTTGATTTATTTTATGAGAATAGTGCTGGTGAACCTCTTGAGTATACTTTACCTTTAATTTGGGACAAGCTGGCCATGTCTTCAAGCTTTAACCAAAGGACTGAAATGGTAAAAGAATTTAATAAATGCAATAAATGGCGGAAAAGAGGAATTTCTCGAGTACCTATAGTATATGAAGTTTCATTGAGACCAACTCCAGGGAAAGTGAGCATTCTAAGCGATGGTTCTGTTGTTGTTGAAGTTGGAGGGATTGAACTGGGTCAGGGTCTCTGGACAAAAGTAAAACAGACGGCTGCTTTCGCTCTTGGTTCCATTCAATGTGATGATAGTGGAGATCTTTTGGATAAAGTACGGGTGGTACAATCTGATACAGTAAGCTTAATTCAAGGAGGATTTACTGCTGGAAGCACTACATCTGAGTCAAGTTGCCAGGCAGTTAGGTTATCCTGTGATATTTTGGTTGAGAGGCTAGCTCCTCTCAAGCAAATATTTCAGGAGCAAATGGGTTCTATTAAATGGGAGACGCTTATTGAAAAGGCATACTTGCAAGCTTTGAACTTATCAGCAAGTTCTTTCTATGTTCCTGATTTTACTTCTATGCAATACCTTAACTATGGTGCTGCAGTGAGTGAGGTTGAGGTAAATCTTCTGACTGGAGAAACTAGAATATTGCAATCAGATATTATCTACGATTGTGGTCAGAGTCTCAACCCTGCTGTGGATTTAGGACAGATCGAAGGTGCTTTTGTTCAAGGAATTGGGTTTTTCATGCTTGAAGAATACATGGAAAATTCTGAGGGATTGGTGATTTCTAATGGTACATGGACATATAAAATTCCTTCCATAGACACTATACCCAAGCAGTTCAATGTCGAAATACTAAATAGTGGACAACACAGTAATCGTGTTCTCTCTTCAAAAGGTATGTCATTCAGATTTTTGCTCAGAGTTCTCATGTTAATGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1301

Amino Acids

142.18

Weight (kDa)

6.32

Isoelectric Point (pI)

37.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	9 - 75	3.4e-07	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	87 - 174	2.7e-21	[2Fe-2S] binding domain
FAD_binding_5	PF00941	235 - 415	1e-38	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	433 - 538	2.6e-19	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	606 - 714	3.7e-28	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	743 - 974	1.1e-80	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1003 - 1265	1.6e-73	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	621, 1790
AccI	GTMKAC	1 cut(s)	2981
AciI	CCGC	7 cut(s)	318, 339, 1319, 2531, 2548, 2818, 3076
AcoI	YGGCCR	2 cut(s)	3, 3009
AcsI	RAATTY	8 cut(s)	1063, 1638, 1919, 2116, 3053, 3087, 3727, 3771
AcuI	CTGAAG	9 cut(s)	285, 471, 761, 1011, 1080, 1383, 1566, 1800, 2874
AflII	CTTAAG	1 cut(s)	2741
AflIII	ACRYGT	1 cut(s)	2869
AjnI	CCWGG	4 cut(s)	1004, 2824, 3133, 3366
AloI	GAACNNNNNNTCC	2 cut(s)	542, 574
Alw21I	GWGCWC	3 cut(s)	2527, 2740, 2764
Alw26I	GTCTC	7 cut(s)	224, 576, 2318, 3118, 3209, 3452, 3647
Alw44I	GTGCAC	2 cut(s)	2523, 2736
Ama87I	CYCGRG	4 cut(s)	80, 353, 425, 3093
AoxI	GGCC	6 cut(s)	3, 402, 417, 2490, 2694, 3009
ApaLI	GTGCAC	2 cut(s)	2523, 2736
ApeKI	GCWGC	8 cut(s)	234, 1307, 1349, 1802, 2111, 2934, 3230, 3557
ApoI	RAATTY	8 cut(s)	1063, 1638, 1919, 2116, 3053, 3087, 3727, 3771
ArsI	GACNNNNNNTTYG	2 cut(s)	3848, 3880
AseI	ATTAAT	1 cut(s)	2678
AspS9I	GGNCC	5 cut(s)	418, 675, 2206, 2695, 2822
AsuC2I	CCSGG	4 cut(s)	354, 355, 426, 427
AsuNHI	GCTAGC	1 cut(s)	3406
AvaI	CYCGRG	4 cut(s)	80, 353, 425, 3093
AvaII	GGWCC	3 cut(s)	675, 2206, 2822
BaeGI	GKGCMC	2 cut(s)	2527, 2740
BalI	TGGCCA	1 cut(s)	3011
BamHI	GGATCC	3 cut(s)	886, 1204, 1271
BanII	GRGCYC	1 cut(s)	1198
BarI	GAAGNNNNNNTAC	2 cut(s)	2606, 2638
BbsI	GAAGAC	3 cut(s)	13, 2361, 3009
Bbv12I	GWGCWC	3 cut(s)	2527, 2740, 2764
BbvI	GCAGC	8 cut(s)	246, 1294, 1336, 1789, 2098, 2946, 3217, 3544
BceAI	ACGGC	2 cut(s)	2477, 3243
BciT130I	CCWGG	4 cut(s)	1006, 2826, 3135, 3368
BclI	TGATCA	1 cut(s)	2266
BcnI	CCSGG	4 cut(s)	354, 355, 426, 427
BcoDI	GTCTC	7 cut(s)	224, 576, 2318, 3118, 3209, 3452, 3647
BfmI	CTRYAG	6 cut(s)	232, 1266, 1300, 2148, 3102, 3558
BfrI	CTTAAG	1 cut(s)	2741
BglII	AGATCT	2 cut(s)	1157, 3271
BisI	GCNGC	9 cut(s)	235, 1308, 1350, 1803, 2112, 2532, 2935, 3231, 3558
BlsI	GCNGC	9 cut(s)	236, 1309, 1351, 1804, 2113, 2533, 2936, 3232, 3559
Bme1390I	CCNGG	8 cut(s)	354, 355, 426, 427, 1006, 2826, 3135, 3368
Bme18I	GGWCC	3 cut(s)	675, 2206, 2822
BmeT110I	CYCGRG	4 cut(s)	80, 353, 425, 3093
BmgT120I	GGNCC	5 cut(s)	418, 675, 2206, 2695, 2822
BmiI	GGNNCC	8 cut(s)	420, 888, 1206, 1273, 1542, 2208, 2823, 3247
BmrFI	CCNGG	8 cut(s)	354, 355, 426, 427, 1006, 2826, 3135, 3368
BmrI	ACTGGG	3 cut(s)	501, 1252, 3203
BmsI	GCATC	5 cut(s)	502, 745, 2614, 2674, 2885
BmtI	GCTAGC	1 cut(s)	3410
BmuI	ACTGGG	3 cut(s)	501, 1252, 3203
BpiI	GAAGAC	3 cut(s)	13, 2361, 3009
BplI	GAGNNNNNCTC	4 cut(s)	3115, 3147, 3394, 3426
BpmI	CTGGAG	4 cut(s)	592, 1818, 3117, 3612
BpuEI	CTTGAG	4 cut(s)	958, 1510, 2996, 3401
BpuMI	CCSGG	4 cut(s)	354, 355, 426, 427
Bsa29I	ATCGAT	1 cut(s)	2682
BsaI	GGTCTC	2 cut(s)	3118, 3209
BsaJI	CCNNGG	5 cut(s)	353, 425, 2825, 2889, 3134
BsaXI	ACNNNNNCTCC	4 cut(s)	900, 930, 2455, 2485
Bse1I	ACTGG	6 cut(s)	496, 1247, 1393, 1772, 3198, 3595
Bse3DI	GCAATG	3 cut(s)	1331, 1711, 2929
BseBI	CCWGG	4 cut(s)	1006, 2826, 3135, 3368
BseCI	ATCGAT	1 cut(s)	2682
BseDI	CCNNGG	5 cut(s)	353, 425, 2825, 2889, 3134
BseGI	GGATG	6 cut(s)	363, 760, 888, 2131, 2605, 2697
BseMI	GCAATG	3 cut(s)	1331, 1711, 2929
BseNI	ACTGG	6 cut(s)	496, 1247, 1393, 1772, 3198, 3595
BseRI	GAGGAG	3 cut(s)	749, 2189, 3402
BseSI	GKGCMC	2 cut(s)	2527, 2740
BseXI	GCAGC	8 cut(s)	246, 1294, 1336, 1789, 2098, 2946, 3217, 3544
BsgI	GTGCAG	3 cut(s)	1293, 1779, 2855
Bsh1285I	CGRYCG	1 cut(s)	409
BshFI	GGCC	6 cut(s)	5, 404, 419, 2492, 2696, 3011
BshVI	ATCGAT	1 cut(s)	2682
BsiEI	CGRYCG	1 cut(s)	409
BsiHKAI	GWGCWC	3 cut(s)	2527, 2740, 2764
BsiHKCI	CYCGRG	4 cut(s)	80, 353, 425, 3093
BsiSI	CCGG	3 cut(s)	6, 354, 426
BsiWI	CGTACG	1 cut(s)	1900
BslFI	GGGAC	4 cut(s)	817, 1447, 2192, 3014
BsmAI	GTCTC	7 cut(s)	224, 576, 2318, 3118, 3209, 3452, 3647
BsmBI	CGTCTC	1 cut(s)	3452
BsmFI	GGGAC	4 cut(s)	817, 1447, 2192, 3014
BsmI	GAATGC	3 cut(s)	1351, 2430, 3147
BsnI	GGCC	6 cut(s)	5, 404, 419, 2492, 2696, 3011
Bso31I	GGTCTC	2 cut(s)	3118, 3209
BsoBI	CYCGRG	4 cut(s)	80, 353, 425, 3093
Bsp1286I	GDGCHC	4 cut(s)	1198, 2527, 2740, 2764
Bsp1407I	TGTACA	1 cut(s)	641
Bsp19I	CCATGG	1 cut(s)	2889
BspACI	CCGC	7 cut(s)	318, 339, 1319, 2531, 2548, 2818, 3076
BspANI	GGCC	6 cut(s)	5, 404, 419, 2492, 2696, 3011
BspDI	ATCGAT	1 cut(s)	2682
BspHI	TCATGA	1 cut(s)	1143
BspLI	GGNNCC	8 cut(s)	420, 888, 1206, 1273, 1542, 2208, 2823, 3247
BspMAI	CTGCAG	3 cut(s)	236, 1270, 3562
BspOI	GCTAGC	1 cut(s)	3410
BspQI	GCTCTTC	1 cut(s)	737
BspTI	CTTAAG	1 cut(s)	2741
BspTNI	GGTCTC	2 cut(s)	3118, 3209
BsrDI	GCAATG	3 cut(s)	1331, 1711, 2929
BsrGI	TGTACA	1 cut(s)	641
BsrI	ACTGG	6 cut(s)	496, 1247, 1393, 1772, 3198, 3595
BssECI	CCNNGG	5 cut(s)	353, 425, 2825, 2889, 3134
BssNAI	GTATAC	1 cut(s)	2982
BssT1I	CCWWGG	1 cut(s)	2889
Bst1107I	GTATAC	1 cut(s)	2982
Bst2UI	CCWGG	4 cut(s)	1006, 2826, 3135, 3368
Bst6I	CTCTTC	2 cut(s)	737, 3856
BstAFI	CTTAAG	1 cut(s)	2741
BstAUI	TGTACA	1 cut(s)	641
BstC8I	GCNNGC	7 cut(s)	517, 594, 1781, 1865, 3009, 3408, 3486
BstDSI	CCRYGG	1 cut(s)	2889
BstENI	CCTNNNNNAGG	2 cut(s)	571, 1548
BstF5I	GGATG	6 cut(s)	363, 760, 888, 2131, 2605, 2697
BstMAI	GTCTC	7 cut(s)	224, 576, 2318, 3118, 3209, 3452, 3647
BstMCI	CGRYCG	1 cut(s)	409
BstNI	CCWGG	4 cut(s)	1006, 2826, 3135, 3368
BstNSI	RCATGY	3 cut(s)	1445, 2523, 2873
BstSCI	CCNGG	8 cut(s)	352, 353, 424, 425, 1004, 2824, 3133, 3366
BstSFI	CTRYAG	6 cut(s)	232, 1266, 1300, 2148, 3102, 3558
BstSLI	GKGCMC	2 cut(s)	2527, 2740
BstV1I	GCAGC	8 cut(s)	246, 1294, 1336, 1789, 2098, 2946, 3217, 3544
BstV2I	GAAGAC	3 cut(s)	13, 2361, 3009
BstX2I	RGATCY	6 cut(s)	886, 1157, 1204, 1271, 2000, 3271
BstXI	CCANNNNNNTGG	2 cut(s)	1012, 2896
BstYI	RGATCY	6 cut(s)	886, 1157, 1204, 1271, 2000, 3271
BstZ17I	GTATAC	1 cut(s)	2982
Bsu15I	ATCGAT	1 cut(s)	2682
BsuRI	GGCC	6 cut(s)	5, 404, 419, 2492, 2696, 3011
BsuTUI	ATCGAT	1 cut(s)	2682
BtgI	CCRYGG	1 cut(s)	2889
BtgZI	GCGATG	3 cut(s)	490, 2398, 3171
BtsCI	GGATG	6 cut(s)	363, 760, 888, 2131, 2605, 2697
BtsI	GCAGTG	2 cut(s)	1405, 3567
BtsIMutI	CAGTG	6 cut(s)	1140, 1405, 1633, 2393, 2632, 3567
Cac8I	GCNNGC	7 cut(s)	517, 594, 1781, 1865, 3009, 3408, 3486
CciI	TCATGA	1 cut(s)	1143
Cfr13I	GGNCC	5 cut(s)	418, 675, 2206, 2695, 2822
Cfr9I	CCCGGG	2 cut(s)	353, 425
ClaI	ATCGAT	1 cut(s)	2682
CseI	GACGC	3 cut(s)	103, 2054, 3469
CspCI	CAANNNNNGTGG	2 cut(s)	2103, 2138
DraI	TTTAAA	3 cut(s)	945, 1476, 1969
EaeI	YGGCCR	2 cut(s)	3, 3009
Eam1104I	CTCTTC	2 cut(s)	737, 3856
EarI	CTCTTC	2 cut(s)	737, 3856
EciI	GGCGGA	1 cut(s)	3091
Eco130I	CCWWGG	1 cut(s)	2889
Eco24I	GRGCYC	1 cut(s)	1198
Eco31I	GGTCTC	2 cut(s)	3118, 3209
Eco32I	GATATC	1 cut(s)	1975
Eco47I	GGWCC	3 cut(s)	675, 2206, 2822
Eco57I	CTGAAG	9 cut(s)	285, 471, 761, 1011, 1080, 1383, 1566, 1800, 2874
Eco88I	CYCGRG	4 cut(s)	80, 353, 425, 3093
EcoNI	CCTNNNNNAGG	2 cut(s)	571, 1548
EcoO109I	RGGNCCY	4 cut(s)	418, 675, 2206, 2822
EcoRII	CCWGG	4 cut(s)	1004, 2824, 3133, 3366
EcoRV	GATATC	1 cut(s)	1975
EcoT14I	CCWWGG	1 cut(s)	2889
EcoT22I	ATGCAT	1 cut(s)	1333
EcoT38I	GRGCYC	1 cut(s)	1198
ErhI	CCWWGG	1 cut(s)	2889
Esp3I	CGTCTC	1 cut(s)	3452
FalI	AAGNNNNNCTT	6 cut(s)	951, 983, 1570, 1602, 3464, 3496
FaqI	GGGAC	4 cut(s)	817, 1447, 2192, 3014
FauI	CCCGC	2 cut(s)	332, 2811
FauNDI	CATATG	2 cut(s)	1011, 1720
FbaI	TGATCA	1 cut(s)	2266
FblI	GTMKAC	1 cut(s)	2981
Fnu4HI	GCNGC	9 cut(s)	235, 1308, 1350, 1803, 2112, 2532, 2935, 3231, 3558
FokI	GGATG	6 cut(s)	370, 767, 895, 2138, 2592, 2704
FriOI	GRGCYC	1 cut(s)	1198
Fsp4HI	GCNGC	9 cut(s)	235, 1308, 1350, 1803, 2112, 2532, 2935, 3231, 3558
GluI	GCNGC	9 cut(s)	235, 1308, 1350, 1803, 2112, 2532, 2935, 3231, 3558
GsuI	CTGGAG	4 cut(s)	592, 1818, 3117, 3612
HaeIII	GGCC	6 cut(s)	5, 404, 419, 2492, 2696, 3011
HapII	CCGG	3 cut(s)	6, 354, 426
HgaI	GACGC	3 cut(s)	103, 2054, 3469
HincII	GTYRAC	2 cut(s)	541, 825
HindII	GTYRAC	2 cut(s)	541, 825
HindIII	AAGCTT	6 cut(s)	939, 1579, 1812, 3022, 3313, 3486
HinfI	GANTC	8 cut(s)	695, 764, 1127, 1561, 1714, 2634, 3356, 3640
HpaI	GTTAAC	1 cut(s)	825
HpaII	CCGG	3 cut(s)	6, 354, 426
Hpy99I	CGWCG	2 cut(s)	688, 895
Ksp22I	TGATCA	1 cut(s)	2266
KspAI	GTTAAC	1 cut(s)	825
LguI	GCTCTTC	1 cut(s)	737
LmnI	GCTCC	6 cut(s)	736, 908, 1799, 1871, 3415, 3433
Lsp1109I	GCAGC	8 cut(s)	246, 1294, 1336, 1789, 2098, 2946, 3217, 3544
LweI	GCATC	5 cut(s)	502, 745, 2614, 2674, 2885
MaeIII	GTNAC	5 cut(s)	794, 916, 1252, 1628, 2237
MfeI	CAATTG	1 cut(s)	912
MflI	RGATCY	6 cut(s)	886, 1157, 1204, 1271, 2000, 3271
MhlI	GDGCHC	4 cut(s)	1198, 2527, 2740, 2764
MlsI	TGGCCA	1 cut(s)	3011
MluNI	TGGCCA	1 cut(s)	3011
MlyI	GAGTC	3 cut(s)	1570, 3365, 3649
MmeI	TCCRAC	4 cut(s)	914, 1155, 1978, 3160
Mox20I	TGGCCA	1 cut(s)	3011
Mph1103I	ATGCAT	1 cut(s)	1333
MscI	TGGCCA	1 cut(s)	3011
MslI	CAYNNNNRTG	2 cut(s)	2894, 3896
Msp20I	TGGCCA	1 cut(s)	3011
MspA1I	CMGCKG	1 cut(s)	318
MspCI	CTTAAG	1 cut(s)	2741
MspI	CCGG	3 cut(s)	6, 354, 426
MspR9I	CCNGG	8 cut(s)	354, 355, 426, 427, 1006, 2826, 3135, 3368
MunI	CAATTG	1 cut(s)	912
Mva1269I	GAATGC	3 cut(s)	1351, 2430, 3147
MvaI	CCWGG	4 cut(s)	1006, 2826, 3135, 3368
NciI	CCSGG	4 cut(s)	354, 355, 426, 427
NcoI	CCATGG	1 cut(s)	2889
NdeI	CATATG	2 cut(s)	1011, 1720
NheI	GCTAGC	1 cut(s)	3406
NlaIV	GGNNCC	8 cut(s)	420, 888, 1206, 1273, 1542, 2208, 2823, 3247
NmuCI	GTSAC	2 cut(s)	1628, 2237
NsiI	ATGCAT	1 cut(s)	1333
NspI	RCATGY	3 cut(s)	1445, 2523, 2873
PaeR7I	CTCGAG	2 cut(s)	80, 3093
PagI	TCATGA	1 cut(s)	1143
PciI	ACATGT	1 cut(s)	2869
PciSI	GCTCTTC	1 cut(s)	737
PctI	GAATGC	3 cut(s)	1351, 2430, 3147
PfeI	GAWTC	5 cut(s)	695, 764, 1127, 1714, 2634
Pfl23II	CGTACG	1 cut(s)	1900
PflFI	GACNNNGTC	1 cut(s)	445
PkrI	GCNGC	9 cut(s)	236, 1309, 1351, 1804, 2113, 2533, 2936, 3232, 3559
Ple19I	CGATCG	1 cut(s)	409
PleI	GAGTC	3 cut(s)	1569, 3364, 3648
PpsI	GAGTC	3 cut(s)	1569, 3364, 3648
PpuMI	RGGWCCY	3 cut(s)	675, 2206, 2822
PscI	ACATGT	1 cut(s)	2869
PshBI	ATTAAT	1 cut(s)	2678
PsiI	TTATAA	2 cut(s)	621, 1790
Psp5II	RGGWCCY	3 cut(s)	675, 2206, 2822
Psp6I	CCWGG	4 cut(s)	1004, 2824, 3133, 3366
PspGI	CCWGG	4 cut(s)	1004, 2824, 3133, 3366
PspLI	CGTACG	1 cut(s)	1900
PspN4I	GGNNCC	8 cut(s)	420, 888, 1206, 1273, 1542, 2208, 2823, 3247
PspPI	GGNCC	5 cut(s)	418, 675, 2206, 2695, 2822
PspPPI	RGGWCCY	3 cut(s)	675, 2206, 2822
PspXI	VCTCGAGB	1 cut(s)	80
PsrI	GAACNNNNNNTAC	2 cut(s)	2145, 2177
PstI	CTGCAG	3 cut(s)	236, 1270, 3562
PsuI	RGATCY	6 cut(s)	886, 1157, 1204, 1271, 2000, 3271
PsyI	GACNNNGTC	1 cut(s)	445
PvuI	CGATCG	1 cut(s)	409
RseI	CAYNNNNRTG	2 cut(s)	2894, 3896
SapI	GCTCTTC	1 cut(s)	737
SatI	GCNGC	9 cut(s)	235, 1308, 1350, 1803, 2112, 2532, 2935, 3231, 3558
Sau96I	GGNCC	5 cut(s)	418, 675, 2206, 2695, 2822
SchI	GAGTC	3 cut(s)	1570, 3365, 3649
ScrFI	CCNGG	8 cut(s)	354, 355, 426, 427, 1006, 2826, 3135, 3368
SduI	GDGCHC	4 cut(s)	1198, 2527, 2740, 2764
SfaNI	GCATC	5 cut(s)	502, 745, 2614, 2674, 2885
SfcI	CTRYAG	6 cut(s)	232, 1266, 1300, 2148, 3102, 3558
Sfr274I	CTCGAG	2 cut(s)	80, 3093
SinI	GGWCC	3 cut(s)	675, 2206, 2822
SlaI	CTCGAG	2 cut(s)	80, 3093
SmaI	CCCGGG	2 cut(s)	355, 427
SmiMI	CAYNNNNRTG	2 cut(s)	2894, 3896
SmlI	CTYRAG	7 cut(s)	80, 973, 1489, 2741, 2975, 3093, 3416
SmoI	CTYRAG	7 cut(s)	80, 973, 1489, 2741, 2975, 3093, 3416
SsiI	CCGC	7 cut(s)	318, 339, 1319, 2531, 2548, 2818, 3076
SspI	AATATT	2 cut(s)	3426, 3606
StyD4I	CCNGG	8 cut(s)	352, 353, 424, 425, 1004, 2824, 3133, 3366
StyI	CCWWGG	1 cut(s)	2889
TaqI	TCGA	8 cut(s)	60, 81, 409, 1528, 2682, 3094, 3672, 3810
TatI	WGTACW	4 cut(s)	347, 488, 641, 1216
TauI	GCSGC	1 cut(s)	2534
TfiI	GAWTC	5 cut(s)	695, 764, 1127, 1714, 2634
TscAI	CASTG	6 cut(s)	1140, 1412, 1633, 2393, 2632, 3567
TseFI	GTSAC	2 cut(s)	1628, 2237
TseI	GCWGC	8 cut(s)	234, 1307, 1349, 1802, 2111, 2934, 3230, 3557
Tsp45I	GTSAC	2 cut(s)	1628, 2237
TspGWI	ACGGA	4 cut(s)	80, 1494, 2158, 2438
TspMI	CCCGGG	2 cut(s)	353, 425
TspRI	CASTG	6 cut(s)	1140, 1412, 1633, 2393, 2632, 3567
Tth111I	GACNNNGTC	1 cut(s)	445
Vha464I	CTTAAG	1 cut(s)	2741
VneI	GTGCAC	2 cut(s)	2523, 2736
VpaK11BI	GGWCC	3 cut(s)	675, 2206, 2822
VspI	ATTAAT	1 cut(s)	2678
XagI	CCTNNNNNAGG	2 cut(s)	571, 1548
XapI	RAATTY	8 cut(s)	1063, 1638, 1919, 2116, 3053, 3087, 3727, 3771
XbaI	TCTAGA	1 cut(s)	1154
XceI	RCATGY	3 cut(s)	1445, 2523, 2873
XhoI	CTCGAG	2 cut(s)	80, 3093
XmaI	CCCGGG	2 cut(s)	353, 425
XmiI	GTMKAC	1 cut(s)	2981
Zsp2I	ATGCAT	1 cut(s)	1333

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.