Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Reverse (-)

21454202 .. 21461501

Gene Structure 7300 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1178500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3432 bp

ATGGCCGACGAAAGAGACGCAGCAGCAGCAAGCGATGGCAGCCTTGTTTTTGCAGTTAACGGGGAGAGGTTTGAGCTCTCGACTGTTGACCCTTCAACCACTTTGCTCCACTTCTTGCGTTCTCAGACGAGATTCGAAAGTGTCAAGCTCAGCTGTGGTGAAGGTGGTTGCGGTGCTTGTGTTGTGCTACTGTCCAAGTACGACCCTGTTGTCGACGAAGTGAAAGATTTTACTGCAAGTTCATGTCTCACTCTGCTTTGTAGTATAAATGGATGTTCAATTACAACATCTGAAGGCCTTGGAAATATCAAAGACGGGTTCCACCCAATTCACCAAAGGATTGCTGGTTTCCATGCTTCTCAATGCGGCTTTTGTACTCCTGGCATGTGTGTTTCACTCTTTGGAGCTCTTGTCAATGCTGAAAAGACGAATAGACCTGATCCCCCTCCTGGGTTCTCCAAGCTTTCTGTTTCCGAAGCTGAAAAGTCTATTGCAGGTAACCTCTGTCGATGTACTGGGTATCGACCGATTGCTGATGCCTGCAAGAGTTTTGCAGCTGATGTTGATATGGAGGATTTGGGGTTCAACTCCTTTTGGAAAAAGGGAGATAGTAAGGAAGTAAAAGTAAAGAGTTTGCCTCCATATAACCGTAATGGAGACATATGTACATTTCCGGACTTCTTAAAGAAAGAAATTCGTTCTTCCATGCCCTTGGATCCTAAGAGATATGGTTGGTACAATCCTGTTAGAGTTGAGGAGCTTCAAAACTTATTGAAAGATAATGATTTTGATAGTGCAAACGAGATGAAATTAGTTGTTGGAAACACAGGGACGGGCTATTACAAGGAACTAAAGCGCTATGACAGATACATTGATCTCAGATGTGTTCCTGAATTATCAATGATTAAGATCGATCCTACGGGGGCTGAGTTCGGAGCAATTGTAACAATCTCTAAAGTTATTGAGTCTTTGAGAAAGAATGACAACGGTGGATCTCCATCAAGAGGTGGTGATGTGTTAAAAAATATTGCCATTCATATGGAGAAAATTGCTTCCGGGTTCATCAGAAATACAGCTAGTATAGGAGGCAATTTGGTGATGGCACAGAGAAAATGTTTTCCTTCAGATATTGCTACAATACTTCTTGCTGTGGATTCAGAGGTAGATATAATGAATGGTGCTAGATCTGAGACGATTATGTTGGAGGATTTTCTGAGAAGGCCTCCACTGGATCCGAAAAGTGTACTTTTAAGTGTTAAAATCCCAAACTGGGAAGCAATTAGAAAAGGTTCTCGAGAAACCAACACCATGTTGCTTTTTGAAACCTATCGAGCTGCACCAAGACCCCTAGGAAGCGCATTGGCTTATTTGAATGCTGCCTTCCTAGCTGAAGTATCGTTTTGGAAGATTTCCAATGGAATTATGGTGGATCATTGTCGTCTGGCTTTTGGTGCTTATGGAACTAAACATGCAATTAGAGCAAGAAAGGTTGAGGAATTTTTAACTGGGAAAGTGCTGAGTCCTGGTGTTCTGTATGACGCTATTAAATTAGTTAGGGATGCTGTGGTTCCTGAAGAAGGAACTACGAGCCCTGCTTACAGGTCAAGCTTGGCTGCTGGTTTTATTTTCGAGTTCTTTAGCCCCTTAATTGACAGTGAAGGTTCCAATGGATTCTTGGGAAATACTTCGTTGGCCAATGCTTCTAAGTTAAAAAGGAACAAAAGCGCTAACGATAAGATGAGAACTGTTCTGTCATCTGGTAAACAGGTGATTGAACTAAGTACAGAGTATGATCCGGTTGGTAAGCCAATTACAAAATCTGGAGGTCTCATACAAGCTTCAGGTGAGGCAGTCTTTGTAGATGACATTCCATCTCCGAAATATTGCTTACATGGAGCGTTCATTTATAGTACAAAGCCTCTGGCTAGAGTGAAGGGAATAAAATTTGAACCTAAACCACATCCAGGAGTTGGTGCACTGATATCTTTTAAAGATATTCCCAAGTCTGGGGAGAACATAGGGTCTAAGACTATGTTTGGTACAGAACCTCTGTTTGCTGACGATCTTACCGAATGTGCTGGTCAGCGTCTTGCCTTCCTGGTAGCCGATACGCAGAAACATGCAGATATGGCTACAAACTTTGCGGTGGTTGATTATGGCATGGAAGATACAGATCCACCTATCTTGTCAGTCGAAGAGGCAGTTAAGAGAGCAAGTTTTTTTGAGGTCCCTCCTTTCTTGTATCCAAAACAGGTTGGTGACATATCAAATGGAATGGCTGCAGCTGATCACAAGATTATTTCTGCTGAGATCAAACTTGGTTCACAATACTATTTCTACATGGAGACACAAACAGCCCTTGCTGTACCAGATGAAGAAAACTGCATGGTGGTTTACACTTCAAGTCAAGGGCCTGAGTATGCCCACTCTACAATTGCAAAATGTCTTGGTATTCCTGAAAATAATATTCGTGTAATTACAAGAAGGGTCGGAGGGGGCTTTGGTGGAAAGGGCATACAATCAGTGCCTGTCGCAACTGCATGTGCTCTTGCAGCACACAAATTACATCGACCAGTGCGGATGTATCTCAATCGCAAGACTGATATGATAATGGCTGGAGGAAGGCATCCCATGAAGATAACTTACAGTGTGGGATTCAAGTCTGATGGGAAGATTACAGCTTTAGAACTTGAGATATTGATAAATGCTGGGATTTCCATAGGCTTTAGTCCAGTATTGCCAAGAACTATTCTGTGGGCACTTAAGAAGTATGACTGGGGTGCTCTGGCTTTCGATATAAAGGTGTGCAAAACAAACACTCCGAGTAGGTCTACTATGCGGGCCCCTGGGGAGGTGCAGGGGTCATTTATCGCTGAAGCTGTAATTGAACATGTAGCATCCACCCTTTCTATAGAAGTAGATTATGTGAGAAGTGTTAATCTTCACACATCTCGCAGCCTTGATTTGTTTTACGAGCACAGCGCTGGTGAACCTCTGGAGTATACTTTACCATTGATATGGAACAAGTTGGCCATGTCTTCAAGCTTTAACCCAAGGACTGAGATGGTAAAAGAATTTAATAGGTGTACTAAATGGCAGAAAAGAGGAATTTCTCGAGTACCGATATTGTGTGAAGTGTCTTTGAGGCCAACTCCGGGAAAAGTAAGCATTTTAAGTGATGGGTCTGTTGTTGTTGAAGTTGGAGGAATTGAACTGGGTCAGGGGCTCTGGACGAAGGTAAAACAGGTGACAGCTTTTGCTCTTGGTTCGATTCAATGCGATGGTAGTGGAGATCTTTCGGATAAAGTACGGGTGGTGCAATCAGATACGTTGAGCTTAATTCAAGGGGGAATTACTGTCGGGAGCACTACATCTCTGAGGCAAGCTGTGAGGCAGTTAGACTTTGCTGTAATATTTTGGTTGAGAGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1144

Amino Acids

124.51

Weight (kDa)

8.0

Isoelectric Point (pI)

36.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	18 - 84	3e-07	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	96 - 182	5.4e-21	[2Fe-2S] binding domain
FAD_binding_5	PF00941	242 - 424	5.2e-38	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	442 - 547	1.4e-19	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	614 - 720	1.8e-25	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	750 - 981	3.8e-81	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1011 - 1125	2.8e-19	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	209
Acc36I	ACCTGC	1 cut(s)	485
AccB7I	CCANNNNNTGG	1 cut(s)	1970
AccI	GTMKAC	3 cut(s)	213, 2831, 3002
AccIII	TCCGGA	1 cut(s)	673
AciI	CCGC	5 cut(s)	171, 366, 2144, 2578, 2839
AcoI	YGGCCR	3 cut(s)	3, 1692, 3030
AcsI	RAATTY	5 cut(s)	693, 1496, 1943, 3074, 3108
AcuI	CTGAAG	6 cut(s)	312, 1107, 1410, 1593, 1824, 2895
AfeI	AGCGCT	3 cut(s)	857, 1726, 2983
AflII	CTTAAG	1 cut(s)	2762
AflIII	ACRYGT	1 cut(s)	2890
AjnI	CCWGG	6 cut(s)	379, 448, 1522, 1963, 2097, 2845
AloI	GAACNNNNNNTCC	2 cut(s)	566, 598
Alw21I	GWGCWC	7 cut(s)	78, 409, 1978, 2548, 2785, 2979, 3368
Alw26I	GTCTC	6 cut(s)	9, 251, 651, 1184, 1832, 2339
Alw44I	GTGCAC	1 cut(s)	1974
AlwNI	CAGNNNCTG	1 cut(s)	2528
Ama87I	CYCGRG	2 cut(s)	1293, 3114
Aor13HI	TCCGGA	1 cut(s)	673
Aor51HI	AGCGCT	3 cut(s)	857, 1726, 2983
AoxI	GGCC	8 cut(s)	3, 295, 1220, 1692, 2411, 2841, 3030, 3146
ApaI	GGGCCC	1 cut(s)	2845
ApaLI	GTGCAC	1 cut(s)	1974
ApoI	RAATTY	5 cut(s)	693, 1496, 1943, 3074, 3108
Asp700I	GAANNNNTTC	2 cut(s)	1288, 1684
AspA2I	CCTAGG	1 cut(s)	1348
AspLEI	GCGC	4 cut(s)	858, 1358, 1727, 2984
AspS9I	GGNCC	4 cut(s)	2227, 2411, 2841, 2842
AsuC2I	CCSGG	2 cut(s)	1057, 3156
AsuHPI	GGTGA	9 cut(s)	170, 323, 1022, 1108, 1780, 1856, 2270, 2999, 3259
AsuII	TTCGAA	1 cut(s)	135
AvaI	CYCGRG	2 cut(s)	1293, 3114
AvaII	GGWCC	1 cut(s)	2227
AvrII	CCTAGG	1 cut(s)	1348
BaeGI	GKGCMC	3 cut(s)	1978, 2761, 2845
BalI	TGGCCA	2 cut(s)	1694, 3032
BamHI	GGATCC	2 cut(s)	715, 1231
BanII	GRGCYC	5 cut(s)	78, 409, 1592, 2845, 3228
BarI	GAAGNNNNNNTAC	2 cut(s)	2627, 2659
BbsI	GAAGAC	1 cut(s)	3030
Bbv12I	GWGCWC	7 cut(s)	78, 409, 1978, 2548, 2785, 2979, 3368
BccI	CCATC	8 cut(s)	29, 1006, 1093, 1879, 2660, 3058, 3173, 3275
BciT130I	CCWGG	6 cut(s)	381, 450, 1524, 1965, 2099, 2847
BciVI	GTATCC	1 cut(s)	2253
BclI	TGATCA	1 cut(s)	2287
BcnI	CCSGG	2 cut(s)	1057, 3156
BcoDI	GTCTC	6 cut(s)	9, 251, 651, 1184, 1832, 2339
BfaI	CTAG	5 cut(s)	1077, 1182, 1349, 1385, 1926
BfmI	CTRYAG	2 cut(s)	2280, 2910
BfoI	RGCGCY	3 cut(s)	859, 1728, 2985
BfrI	CTTAAG	1 cut(s)	2762
BfuAI	ACCTGC	1 cut(s)	485
BfuI	GTATCC	1 cut(s)	2253
BglII	AGATCT	2 cut(s)	1184, 3292
BlnI	CCTAGG	1 cut(s)	1348
BlpI	GCTNAGC	1 cut(s)	149
Bme1390I	CCNGG	8 cut(s)	381, 450, 1057, 1524, 1965, 2099, 2847, 3156
Bme18I	GGWCC	1 cut(s)	2227
BmeT110I	CYCGRG	2 cut(s)	1293, 3114
BmgT120I	GGNCC	4 cut(s)	2227, 2411, 2841, 2842
BmiI	GGNNCC	8 cut(s)	320, 717, 1233, 1569, 1663, 2229, 2843, 2844
BmrFI	CCNGG	8 cut(s)	381, 450, 1057, 1524, 1965, 2099, 2847, 3156
BmrI	ACTGGG	5 cut(s)	525, 1279, 1515, 2785, 3224
BmsI	GCATC	4 cut(s)	526, 1549, 2635, 2906
BmuI	ACTGGG	5 cut(s)	525, 1279, 1515, 2785, 3224
BpiI	GAAGAC	1 cut(s)	3030
BplI	GAGNNNNNCTC	6 cut(s)	622, 654, 1207, 1239, 3136, 3168
BpmI	CTGGAG	3 cut(s)	1842, 2637, 3017
Bpu1102I	GCTNAGC	1 cut(s)	149
Bpu14I	TTCGAA	1 cut(s)	135
BpuEI	CTTGAG	1 cut(s)	2711
BpuMI	CCSGG	2 cut(s)	1057, 3156
Bsa29I	ATCGAT	1 cut(s)	912
BsaBI	GATNNNNATC	3 cut(s)	908, 997, 2172
BsaI	GGTCTC	1 cut(s)	1832
BsaJI	CCNNGG	7 cut(s)	298, 449, 711, 1348, 2845, 2846, 3053
BsaWI	WCCGGW	2 cut(s)	673, 1795
BsaXI	ACNNNNNCTCC	6 cut(s)	396, 426, 927, 957, 2803, 2833
Bse1I	ACTGG	8 cut(s)	520, 1233, 1274, 1510, 2573, 2732, 2780, 3219
Bse8I	GATNNNNATC	3 cut(s)	908, 997, 2172
BseAI	TCCGGA	1 cut(s)	673
BseBI	CCWGG	6 cut(s)	381, 450, 1524, 1965, 2099, 2847
BseCI	ATCGAT	1 cut(s)	912
BseDI	CCNNGG	7 cut(s)	298, 449, 711, 1348, 2845, 2846, 3053
BseGI	GGATG	6 cut(s)	278, 1564, 1960, 2586, 2626, 2897
BseJI	GATNNNNATC	3 cut(s)	908, 997, 2172
BseNI	ACTGG	8 cut(s)	520, 1233, 1274, 1510, 2573, 2732, 2780, 3219
BseRI	GAGGAG	1 cut(s)	770
BseSI	GKGCMC	3 cut(s)	1978, 2761, 2845
BseYI	CCCAGC	1 cut(s)	2708
BsgI	GTGCAG	2 cut(s)	1320, 2876
Bsh1285I	CGRYCG	1 cut(s)	527
BshFI	GGCC	8 cut(s)	5, 297, 1222, 1694, 2413, 2843, 3032, 3148
BshVI	ATCGAT	1 cut(s)	912
BsiEI	CGRYCG	1 cut(s)	527
BsiHKAI	GWGCWC	7 cut(s)	78, 409, 1978, 2548, 2785, 2979, 3368
BsiHKCI	CYCGRG	2 cut(s)	1293, 3114
BsiSI	CCGG	4 cut(s)	674, 1056, 1796, 3155
BslFI	GGGAC	2 cut(s)	844, 2213
BsmAI	GTCTC	6 cut(s)	9, 251, 651, 1184, 1832, 2339
BsmBI	CGTCTC	2 cut(s)	9, 1184
BsmFI	GGGAC	2 cut(s)	844, 2213
BsmI	GAATGC	1 cut(s)	1378
BsnI	GGCC	8 cut(s)	5, 297, 1222, 1694, 2413, 2843, 3032, 3148
Bso31I	GGTCTC	1 cut(s)	1832
BsoBI	CYCGRG	2 cut(s)	1293, 3114
Bsp119I	TTCGAA	1 cut(s)	135
Bsp120I	GGGCCC	1 cut(s)	2841
Bsp13I	TCCGGA	1 cut(s)	673
Bsp1407I	TGTACA	1 cut(s)	665
Bsp1720I	GCTNAGC	1 cut(s)	149
BspACI	CCGC	5 cut(s)	171, 366, 2144, 2578, 2839
BspANI	GGCC	8 cut(s)	5, 297, 1222, 1694, 2413, 2843, 3032, 3148
BspDI	ATCGAT	1 cut(s)	912
BspEI	TCCGGA	1 cut(s)	673
BspLI	GGNNCC	8 cut(s)	320, 717, 1233, 1569, 1663, 2229, 2843, 2844
BspMAI	CTGCAG	1 cut(s)	2284
BspMI	ACCTGC	1 cut(s)	485
BspT104I	TTCGAA	1 cut(s)	135
BspTI	CTTAAG	1 cut(s)	2762
BspTNI	GGTCTC	1 cut(s)	1832
BsrGI	TGTACA	1 cut(s)	665
BsrI	ACTGG	8 cut(s)	520, 1233, 1274, 1510, 2573, 2732, 2780, 3219
BssECI	CCNNGG	7 cut(s)	298, 449, 711, 1348, 2845, 2846, 3053
BssNAI	GTATAC	1 cut(s)	3003
BssT1I	CCWWGG	4 cut(s)	298, 711, 1348, 3053
Bst1107I	GTATAC	1 cut(s)	3003
Bst2UI	CCWGG	6 cut(s)	381, 450, 1524, 1965, 2099, 2847
Bst4CI	ACNGT	8 cut(s)	85, 192, 650, 989, 1655, 1747, 2648, 3358
Bst6I	CTCTTC	1 cut(s)	2190
BstAFI	CTTAAG	1 cut(s)	2762
BstAUI	TGTACA	1 cut(s)	665
BstBI	TTCGAA	1 cut(s)	135
BstC8I	GCNNGC	4 cut(s)	31, 541, 2841, 3384
BstEII	GGTNACC	1 cut(s)	497
BstENI	CCTNNNNNAGG	1 cut(s)	1575
BstF5I	GGATG	6 cut(s)	278, 1564, 1960, 2586, 2626, 2897
BstH2I	RGCGCY	3 cut(s)	859, 1728, 2985
BstHHI	GCGC	4 cut(s)	858, 1358, 1727, 2984
BstMAI	GTCTC	6 cut(s)	9, 251, 651, 1184, 1832, 2339
BstMCI	CGRYCG	1 cut(s)	527
BstMWI	GCNNNNNNNGC	7 cut(s)	26, 39, 1385, 1451, 1478, 2129, 2552
BstNI	CCWGG	6 cut(s)	381, 450, 1524, 1965, 2099, 2847
BstNSI	RCATGY	5 cut(s)	388, 1472, 2123, 2544, 2894
BstPI	GGTNACC	1 cut(s)	497
BstSCI	CCNGG	8 cut(s)	379, 448, 1055, 1522, 1963, 2097, 2845, 3154
BstSFI	CTRYAG	2 cut(s)	2280, 2910
BstSLI	GKGCMC	3 cut(s)	1978, 2761, 2845
BstV2I	GAAGAC	1 cut(s)	3030
BstX2I	RGATCY	6 cut(s)	715, 992, 1184, 1231, 2173, 3292
BstXI	CCANNNNNNTGG	3 cut(s)	712, 1039, 3018
BstYI	RGATCY	6 cut(s)	715, 992, 1184, 1231, 2173, 3292
BstZ17I	GTATAC	1 cut(s)	3003
Bsu15I	ATCGAT	1 cut(s)	912
BsuI	GTATCC	1 cut(s)	2253
BsuRI	GGCC	8 cut(s)	5, 297, 1222, 1694, 2413, 2843, 3032, 3148
BsuTUI	ATCGAT	1 cut(s)	912
BtgZI	GCGATG	2 cut(s)	48, 3294
BtsCI	GGATG	6 cut(s)	278, 1564, 1960, 2586, 2626, 2897
BtsIMutI	CAGTG	6 cut(s)	1226, 1660, 1976, 2529, 2580, 2653
BveI	ACCTGC	1 cut(s)	485
Cac8I	GCNNGC	4 cut(s)	31, 541, 2841, 3384
CaiI	CAGNNNCTG	1 cut(s)	2528
CfoI	GCGC	4 cut(s)	858, 1358, 1727, 2984
Cfr13I	GGNCC	4 cut(s)	2227, 2411, 2841, 2842
ClaI	ATCGAT	1 cut(s)	912
CseI	GACGC	3 cut(s)	26, 1547, 2075
CspCI	CAANNNNNGTGG	2 cut(s)	2166, 2201
DraI	TTTAAA	1 cut(s)	1990
DrdI	GACNNNNNNGTC	1 cut(s)	209
DseDI	GACNNNNNNGTC	1 cut(s)	209
EaeI	YGGCCR	3 cut(s)	3, 1692, 3030
Eam1104I	CTCTTC	1 cut(s)	2190
EarI	CTCTTC	1 cut(s)	2190
Ecl136II	GAGCTC	2 cut(s)	76, 407
Eco130I	CCWWGG	4 cut(s)	298, 711, 1348, 3053
Eco147I	AGGCCT	2 cut(s)	297, 1222
Eco24I	GRGCYC	5 cut(s)	78, 409, 1592, 2845, 3228
Eco31I	GGTCTC	1 cut(s)	1832
Eco32I	GATATC	1 cut(s)	1983
Eco47I	GGWCC	1 cut(s)	2227
Eco47III	AGCGCT	3 cut(s)	857, 1726, 2983
Eco53kI	GAGCTC	2 cut(s)	76, 407
Eco57I	CTGAAG	6 cut(s)	312, 1107, 1410, 1593, 1824, 2895
Eco88I	CYCGRG	2 cut(s)	1293, 3114
Eco91I	GGTNACC	1 cut(s)	497
EcoICRI	GAGCTC	2 cut(s)	76, 407
EcoNI	CCTNNNNNAGG	1 cut(s)	1575
EcoO109I	RGGNCCY	3 cut(s)	2227, 2411, 2842
EcoO65I	GGTNACC	1 cut(s)	497
EcoRII	CCWGG	6 cut(s)	379, 448, 1522, 1963, 2097, 2845
EcoRV	GATATC	1 cut(s)	1983
EcoT14I	CCWWGG	4 cut(s)	298, 711, 1348, 3053
EcoT38I	GRGCYC	5 cut(s)	78, 409, 1592, 2845, 3228
ErhI	CCWWGG	4 cut(s)	298, 711, 1348, 3053
Esp3I	CGTCTC	2 cut(s)	9, 1184
FaqI	GGGAC	2 cut(s)	844, 2213
FauI	CCCGC	1 cut(s)	2832
FauNDI	CATATG	2 cut(s)	662, 1038
FbaI	TGATCA	1 cut(s)	2287
FblI	GTMKAC	3 cut(s)	213, 2831, 3002
FokI	GGATG	6 cut(s)	285, 1571, 1947, 2593, 2613, 2884
FriOI	GRGCYC	5 cut(s)	78, 409, 1592, 2845, 3228
FspBI	CTAG	5 cut(s)	1077, 1182, 1349, 1385, 1926
GlaI	GCGC	4 cut(s)	857, 1357, 1726, 2983
GsaI	CCCAGC	1 cut(s)	2712
GsuI	CTGGAG	3 cut(s)	1842, 2637, 3017
HaeII	RGCGCY	3 cut(s)	859, 1728, 2985
HaeIII	GGCC	8 cut(s)	5, 297, 1222, 1694, 2413, 2843, 3032, 3148
HapII	CCGG	4 cut(s)	674, 1056, 1796, 3155
HgaI	GACGC	3 cut(s)	26, 1547, 2075
HhaI	GCGC	4 cut(s)	858, 1358, 1727, 2984
Hin6I	GCGC	4 cut(s)	856, 1356, 1725, 2982
HinP1I	GCGC	4 cut(s)	856, 1356, 1725, 2982
HincII	GTYRAC	3 cut(s)	58, 88, 214
HindII	GTYRAC	3 cut(s)	58, 88, 214
HindIII	AAGCTT	4 cut(s)	461, 1606, 1836, 3043
HinfI	GANTC	7 cut(s)	132, 965, 1154, 1519, 1671, 2655, 3271
HpaI	GTTAAC	1 cut(s)	58
HpaII	CCGG	4 cut(s)	674, 1056, 1796, 3155
HphI	GGTGA	9 cut(s)	170, 323, 1022, 1108, 1780, 1856, 2270, 2999, 3259
Hpy99I	CGWCG	2 cut(s)	11, 218
HpyCH4III	ACNGT	8 cut(s)	85, 192, 650, 989, 1655, 1747, 2648, 3358
HpyCH4IV	ACGT	1 cut(s)	3329
HpyF10VI	GCNNNNNNNGC	7 cut(s)	26, 39, 1385, 1451, 1478, 2129, 2552
HpySE526I	ACGT	1 cut(s)	3329
HspAI	GCGC	4 cut(s)	856, 1356, 1725, 2982
Kpn2I	TCCGGA	1 cut(s)	673
Ksp22I	TGATCA	1 cut(s)	2287
KspAI	GTTAAC	1 cut(s)	58
LmnI	GCTCC	6 cut(s)	111, 404, 757, 935, 1895, 3363
LweI	GCATC	4 cut(s)	526, 1549, 2635, 2906
MaeI	CTAG	5 cut(s)	1077, 1182, 1349, 1385, 1926
MaeII	ACGT	1 cut(s)	3329
MaeIII	GTNAC	4 cut(s)	497, 943, 2258, 3247
MfeI	CAATTG	2 cut(s)	939, 2433
MflI	RGATCY	6 cut(s)	715, 992, 1184, 1231, 2173, 3292
MlsI	TGGCCA	2 cut(s)	1694, 3032
MluNI	TGGCCA	2 cut(s)	1694, 3032
MlyI	GAGTC	2 cut(s)	974, 1528
MmeI	TCCRAC	4 cut(s)	799, 1182, 2470, 3181
Mox20I	TGGCCA	2 cut(s)	1694, 3032
MroI	TCCGGA	1 cut(s)	673
MroXI	GAANNNNTTC	2 cut(s)	1288, 1684
MscI	TGGCCA	2 cut(s)	1694, 3032
MslI	CAYNNNNRTG	2 cut(s)	1037, 3016
Msp20I	TGGCCA	2 cut(s)	1694, 3032
MspA1I	CMGCKG	3 cut(s)	153, 557, 2285
MspCI	CTTAAG	1 cut(s)	2762
MspI	CCGG	4 cut(s)	674, 1056, 1796, 3155
MspR9I	CCNGG	8 cut(s)	381, 450, 1057, 1524, 1965, 2099, 2847, 3156
MunI	CAATTG	2 cut(s)	939, 2433
Mva1269I	GAATGC	1 cut(s)	1378
MvaI	CCWGG	6 cut(s)	381, 450, 1524, 1965, 2099, 2847
MwoI	GCNNNNNNNGC	7 cut(s)	26, 39, 1385, 1451, 1478, 2129, 2552
NciI	CCSGG	2 cut(s)	1057, 3156
NdeI	CATATG	2 cut(s)	662, 1038
NlaIV	GGNNCC	8 cut(s)	320, 717, 1233, 1569, 1663, 2229, 2843, 2844
NmuCI	GTSAC	2 cut(s)	2258, 3247
NspI	RCATGY	5 cut(s)	388, 1472, 2123, 2544, 2894
NspV	TTCGAA	1 cut(s)	135
PaeR7I	CTCGAG	2 cut(s)	1293, 3114
PasI	CCCWGGG	1 cut(s)	2846
PceI	AGGCCT	2 cut(s)	297, 1222
PciI	ACATGT	1 cut(s)	2890
PcsI	WCGNNNNNNNCGW	1 cut(s)	2067
PctI	GAATGC	1 cut(s)	1378
PdmI	GAANNNNTTC	2 cut(s)	1288, 1684
PfeI	GAWTC	5 cut(s)	132, 1154, 1671, 2655, 3271
PflMI	CCANNNNNTGG	1 cut(s)	1970
PfoI	TCCNGGA	2 cut(s)	1963, 3154
PleI	GAGTC	2 cut(s)	973, 1527
PpsI	GAGTC	2 cut(s)	973, 1527
PpuMI	RGGWCCY	1 cut(s)	2227
PscI	ACATGT	1 cut(s)	2890
Psp124BI	GAGCTC	2 cut(s)	78, 409
Psp5II	RGGWCCY	1 cut(s)	2227
Psp6I	CCWGG	6 cut(s)	379, 448, 1522, 1963, 2097, 2845
PspEI	GGTNACC	1 cut(s)	497
PspFI	CCCAGC	1 cut(s)	2708
PspGI	CCWGG	6 cut(s)	379, 448, 1522, 1963, 2097, 2845
PspN4I	GGNNCC	8 cut(s)	320, 717, 1233, 1569, 1663, 2229, 2843, 2844
PspOMI	GGGCCC	1 cut(s)	2841
PspPI	GGNCC	4 cut(s)	2227, 2411, 2841, 2842
PspPPI	RGGWCCY	1 cut(s)	2227
PstI	CTGCAG	1 cut(s)	2284
PstNI	CAGNNNCTG	1 cut(s)	2528
PsuI	RGATCY	6 cut(s)	715, 992, 1184, 1231, 2173, 3292
PvuII	CAGCTG	3 cut(s)	153, 557, 2285
RseI	CAYNNNNRTG	2 cut(s)	1037, 3016
SacI	GAGCTC	2 cut(s)	78, 409
SalI	GTCGAC	1 cut(s)	212
Sau96I	GGNCC	4 cut(s)	2227, 2411, 2841, 2842
SchI	GAGTC	2 cut(s)	974, 1528
ScrFI	CCNGG	8 cut(s)	381, 450, 1057, 1524, 1965, 2099, 2847, 3156
SfaNI	GCATC	4 cut(s)	526, 1549, 2635, 2906
SfcI	CTRYAG	2 cut(s)	2280, 2910
Sfr274I	CTCGAG	2 cut(s)	1293, 3114
SfuI	TTCGAA	1 cut(s)	135
SinI	GGWCC	1 cut(s)	2227
SlaI	CTCGAG	2 cut(s)	1293, 3114
SmiMI	CAYNNNNRTG	2 cut(s)	1037, 3016
SmlI	CTYRAG	4 cut(s)	1293, 2690, 2762, 3114
SmoI	CTYRAG	4 cut(s)	1293, 2690, 2762, 3114
SseBI	AGGCCT	2 cut(s)	297, 1222
SsiI	CCGC	5 cut(s)	171, 366, 2144, 2578, 2839
SspI	AATATT	4 cut(s)	1027, 1883, 2467, 3414
SspMI	CTAG	5 cut(s)	1077, 1182, 1349, 1385, 1926
SstI	GAGCTC	2 cut(s)	78, 409
StuI	AGGCCT	2 cut(s)	297, 1222
StyD4I	CCNGG	8 cut(s)	379, 448, 1055, 1522, 1963, 2097, 2845, 3154
StyI	CCWWGG	4 cut(s)	298, 711, 1348, 3053
TaaI	ACNGT	8 cut(s)	85, 192, 650, 989, 1655, 1747, 2648, 3358
TaiI	ACGT	1 cut(s)	3332
TaqII	GACCGA	1 cut(s)	541
TatI	WGTACW	7 cut(s)	374, 512, 665, 1243, 1781, 1910, 3086
TauI	GCSGC	1 cut(s)	369
TfiI	GAWTC	5 cut(s)	132, 1154, 1671, 2655, 3271
TscAI	CASTG	6 cut(s)	1233, 1660, 1983, 2529, 2580, 2653
TseFI	GTSAC	2 cut(s)	2258, 3247
Tsp45I	GTSAC	2 cut(s)	2258, 3247
TspDTI	ATGAA	8 cut(s)	231, 821, 1025, 1051, 1187, 1891, 2388, 2648
TspRI	CASTG	6 cut(s)	1233, 1660, 1983, 2529, 2580, 2653
Van91I	CCANNNNNTGG	1 cut(s)	1970
Vha464I	CTTAAG	1 cut(s)	2762
VneI	GTGCAC	1 cut(s)	1974
VpaK11BI	GGWCC	1 cut(s)	2227
XagI	CCTNNNNNAGG	1 cut(s)	1575
XapI	RAATTY	5 cut(s)	693, 1496, 1943, 3074, 3108
XceI	RCATGY	5 cut(s)	388, 1472, 2123, 2544, 2894
XcmI	CCANNNNNNNNNTGG	3 cut(s)	341, 1420, 1672
XhoI	CTCGAG	2 cut(s)	1293, 3114
XmaJI	CCTAGG	1 cut(s)	1348
XmiI	GTMKAC	3 cut(s)	213, 2831, 3002
XmnI	GAANNNNTTC	2 cut(s)	1288, 1684
XspI	CTAG	5 cut(s)	1077, 1182, 1349, 1385, 1926

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.