Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Forward (+)

11601727 .. 11608450

Gene Structure 6724 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G27150.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3999 bp

ATGGATTTGGAGTTTGCAGTTAATGGGGAACGATTCAAGATTGATTCTGTTGATCCATCAACGACCTTACTTGAGTTCTTGCGATTGAACACTCCTTTCAAGAGTGTCAAGCTCGGCTGTGGCGAAGGAGGGTGTGGTGCTTGTCTTGTAGTGTTATCTAAATATGATCCAGAGTTAGATCAAGTGAAGGAATGTTGTATAAACTCTTGTCTCACTCTTCTCTGTAGTGTAAATGGATGTTCCATTACTACATCTGAAGGTCTTGGAAACACGAAGAAAGGATTTCATCCGATTCATAAGCGGTTCGCGGGTTTTCACGCCTCTCAATGCGGGTTTTGTACACCAGGGATGTGTATTTCTCTCTACTCATCTCTTGCAAATGCTGAGAATAACAGCTCTAAAGATTTTACGGTTTCTGAAGCTGAGAAATCTGTTTCGGGTAATCTTTGTCGATGTACTGGTTATCGTCCCATTGTCGATGCTTGTAAGAGTTTTGCTTCTGATGTTGATATTGAGGACTTAGGGTTAAATTCCTTTTGGAAAAAAGGAGAAAGCAAAGAGGTTATGTTTAAGAATTTGCCTCCTTATAACCCCAAAGACCATCTTGTAACATTCCCTGAGTTCTTGAAGAAGAAAGAGAAGGTTGATAATGGTTCGGATCATTTGAAGTATCGTTGGACCACTCCTTTTAGTGTGGCTGAGCTTCATAACATAATGGAAGCAGCTAACTCTGGAGACTCGTTGAAGTTAGTTGTAGGCAACACGGGTACAGGTTACTATAAAGACGAGGAACGGTTTGATAGATATATTGATATCAGTAACATTCCGGAGATGTCAATGATCAAGAAAGATGAGAAAGGAATTGAAATAGGAGCAGCTGTTACTATATCGAATGCTATTGATGCTTTGGAGAAGGAAAGCAAATCTTCTTATGTTTTCAAGAAAATGGCCACTCATATGGAGAAAATTGGCAACCGTTCTATCCGAAACTCGGGTAGTATTGGTGGTAATCTAGTGATGGCACAGAGCAGAAAGTTTCCTTCTGATGTTACCACTTTGTTGCTCGCTGTAGATGCCTCGGTGTATATGTTAAATGGTAGGAAAACCGAGAAGGTTACGCTACAGGAGTTTCTTGAATTGTCTCCGGTGTTAGACTCCAAACGCGTTCTTTTAAAGGTTGAGATTCCATCATGGACTGCTCCTTCTGGTGATGACACTGAATTTCTCTTTGAAAGCTATCGAGCCGCACCTCGTTCTATTGGAAATGCATTGCCTTACTTGAACGCTGCTTTCTTGGCGCTAGTGTCTCGCCAAGAAGCATCAAGAAAAGGTGTAACGGTAGAAAAATGCTTCCTGGCATTTGGTTCTTATGGCGGGGATCATTCGATCAGGGCGATAGAAGTGGAGACTTTCTTGACAGGTAAATTACTTAGCTACAGTGTTCTATATGAAGCTGTTGGTTTACTTAAAGGAATCATAGTCCCTGGCAAAGACACCTTGCACTCGGAATATAGAAAAAGCTTGGCTGTTGGATATCTTTTTGAGTTTTTTTACCCACTGATCGAGAGTGGCCATAGAATATGTAGTCTTGATTCAGGGAATAAGCATAACAACAGCCATGTTGATACCGTAAAATCTCTTCCCTTCCTCTCATCTTCACAGCAAGTATTAGAGAGCAATGAATTTAAACCTATTGGTGAAGCAGTTATCAAAGTTGGAGCTGCCTTACAAGCTTCTGGTGAGGCGGTTTTTGTTGATGATATACCGACTTTACCAGACTGTCTTCATGGAGCATTCATTTATAGCACAGAGCCTTTGGCTAAGATAAAAAGCTTAAGCTTCAGAGAGAATGTAACTCCTACTGGAGTTTTTGCTGTTCTTACTTTCAAGGATATTCCACAACAAGGACAAAACATTGGTTCCAAGACCCTTTTTGGACCAGGACCTTTATTTGCAGATGAACTAACTCGATGTGCAGGTCAAAGAATTGCTCTTGTGGTTGCAGACACACAGAAACATGCAGACATGGCTGCAAAACTTGCAGTAGTTGAGTATGATACAAAGAATTTAGAACAACCGATATTAACTGTCGAAGATGCAGTTAAAAGATCTAGCTTTTTCGAGGTTCATCCGATGTTTTACCCCGAACCAGTAGGTGATGTTATAAAAGGAATGGAAGAAGCTGAGCGAAAGATAATCTCCTCTGAGTTGAGGCTAGGGTCACAGTACTTCTTCTATATGGAGCCACAAACAGCACTTGCCTTGCCAGACGAAGACAATTGTGTCAAGGTATTTAGTTCATCTCAAGCACCTGAGTACGTACATTCGGTTATTGCTACATGTCTTGGCATTCAAGAGCATAACGTGAGAGTCATCACTAGAAGAGTTGGTGGTGGCTTTGGTGGTAAAGCTGTAAAGTCAATGCCTGTTGCAACCGCATGCGCACTAGGCGCATACAAGTTGCAACGTCCTGTTAAGATGTTTTTGAACCGCAAGACGGATATGATAATGGCTGGAGGAAGACATCCGATGAAGATAAATTACAATGTGGGATTTAGATCAGATGGGAAGCTCACAGCACTGGAGCTTACAATGCTTATAGATGCAGGGCTTGAGCCAGACGTAAGTCCAATCATGCCGCGGAATATAATGGGTCCGCTGAGGAAGTATGACTGGGGAGCTTTGTCATTTGATGTAAAAGTGTGTAAGACGAATTGTCTGAGTAGAACAGCGATGAGAGCTCCCGGGGAAGTTCAAGGTTCTTACATAGCGGAATCCATTATCGAGAATGTAGCTTCTTCTCTTCAAATGGATGTTGATGCAGTGAGAAAAATAAACCTTCATACTTATGATAGCCTTAGAAAATTCTACAATCACATTGCTGGTGATCCTGATGAATATACACTGCCTTTATTATGGGAGAAACTTGAGATATCTTCAAAGTTCAAGGAAAGATCTGAGATGGTGAAGGAGTTTAATCTTTGTAATGTATGGCGAAAGAGAGGGATTTCTCGAGTACCTATAGTCCATCAAGTTATGCAGAGACCGACCCCGGGGAAAGTAAGCATACTGAGTGATGGTTCTGTTGTAGTTGAGGTTGGAGGAATTGAGATAGGGCAAGGATTGTGGACTAAAGTACAACAGATGGTTGCTTATGGTCTCGGTATGGTTAAATGCGAAGGAAACGAAAAGCTCTTGGATAGAATACGTGTAGTTCAGTCCGATACACTAGGTATGATCCAAGGAGGTTTCACTGCTGGTAGCACGACATCCGAGAGCAGTTGTGAAGCCGTTAGGCTTTGCTGTGTTATCTTGGTGGAGAGATTGAAACCTATAATGGATCAGATGATGATGGAGAAGTCAGGTTCCGTGACATGGAACATACTCATTCAACAAGCGTATGGTCAGTATATTAATTTATCGGCGAGTACATTGTATAAGCCAGAGTATTCTTCCATGGAATATCTCAACTATGGAGTTGGAGTCAGCGAGGTGGAAGTGGACCTTGTGACAGGAAAAACCGAGATTTTAAGATCCGATATTATTTATGACTGTGGAAAGAGTCTTAATCCTGCAGTTGATTTAGGACAGACCGAAGGAGCATTTGTTCAAGGCATCGGGTTTTTCATGATGGAAGAGTACACTACTGATGAGAAGGGGCTTGTGGTGCAACAAGGCACTTGGGACTACAAAATACCGACTGTGGACACAATCCCAAAACACTTCAACGTTGAGATTGTCAACACTGGTCATCATAAAAACCGCGTTCTCTCCTCCAAAGCGTCGGGTGAGCCGCCATTGCTTTTAGCAGCTTCTGTTCATTGTGCAACAAGATCAGCCATTAGGGAAGCTCGGAAACATTCCCTTTCATCGAACTTCATTGATGGGTCTGATTCGGAATTTGAGTTACCTGTTCCAGCGACTATGCCTGTGGTGAAGTCTCTTTGCGGATTGTATAGTGTAGAGAAATACTTACAAGGAAAGATCAAAGGACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1332

Amino Acids

146.7

Weight (kDa)

6.23

Isoelectric Point (pI)

36.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	6 - 74	3.7e-08	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	84 - 163	2.1e-24	[2Fe-2S] binding domain
FAD_binding_5	PF00941	223 - 398	9.4e-42	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	412 - 518	2.2e-16	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	579 - 688	4.3e-32	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	718 - 947	2.9e-79	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	977 - 1239	1.1e-74	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	588, 2165
AasI	GACNNNNNNGTC	1 cut(s)	2282
Acc16I	TGCGCA	1 cut(s)	2443
Acc36I	ACCTGC	1 cut(s)	1967
AccII	CGCG	4 cut(s)	308, 1164, 2641, 3765
AccIII	TCCGGA	1 cut(s)	826
AclI	AACGTT	1 cut(s)	3729
AclWI	GGATC	8 cut(s)	47, 161, 666, 1386, 2882, 3232, 3348, 3528
AcoI	YGGCCR	2 cut(s)	948, 1570
AcsI	RAATTY	7 cut(s)	529, 574, 1220, 1682, 2065, 2864, 3899
AcuI	CTGAAG	3 cut(s)	276, 438, 1825
AfiI	CCNNNNNNNGG	5 cut(s)	991, 1904, 2497, 3053, 3375
AflII	CTTAAG	1 cut(s)	1834
AflIII	ACRYGT	3 cut(s)	1162, 2339, 3208
AhdI	GACNNNNNGTC	1 cut(s)	2715
AjnI	CCWGG	4 cut(s)	343, 1353, 1483, 1939
AloI	GAACNNNNNNTCC	2 cut(s)	3591, 3623
Alw21I	GWGCWC	1 cut(s)	2743
Alw26I	GTCTC	8 cut(s)	215, 729, 1146, 1311, 1400, 3037, 3164, 3945
AlwI	GGATC	8 cut(s)	47, 161, 666, 1386, 2882, 3232, 3348, 3528
AlwNI	CAGNNNCTG	1 cut(s)	3815
Ama87I	CYCGRG	4 cut(s)	991, 2744, 3012, 3052
Aor13HI	TCCGGA	1 cut(s)	826
AoxI	GGCC	2 cut(s)	948, 1570
ApeKI	GCWGC	6 cut(s)	722, 875, 1286, 1721, 2030, 3809
ApoI	RAATTY	7 cut(s)	529, 574, 1220, 1682, 2065, 2864, 3899
AseI	ATTAAT	1 cut(s)	3414
AspLEI	GCGC	3 cut(s)	1300, 2444, 2453
AspS9I	GGNCC	5 cut(s)	678, 1937, 1943, 2654, 3502
AsuC2I	CCSGG	4 cut(s)	2745, 2746, 3053, 3054
AsuHPI	GGTGA	8 cut(s)	1220, 1709, 1751, 2168, 2897, 2977, 3800, 3946
AvaI	CYCGRG	4 cut(s)	991, 2744, 3012, 3052
AvaII	GGWCC	5 cut(s)	678, 1937, 1943, 2654, 3502
BalI	TGGCCA	2 cut(s)	950, 1572
BanII	GRGCYC	1 cut(s)	2743
BarI	GAAGNNNNNNTAC	4 cut(s)	1623, 1655, 2525, 2557
BbsI	GAAGAC	3 cut(s)	1775, 2280, 2527
Bbv12I	GWGCWC	1 cut(s)	2743
BbvCI	CCTCAGC	1 cut(s)	2660
BbvI	GCAGC	6 cut(s)	734, 887, 1273, 1708, 2017, 3821
BceAI	ACGGC	1 cut(s)	3275
BciT130I	CCWGG	4 cut(s)	345, 1355, 1485, 1941
BclI	TGATCA	1 cut(s)	840
BcnI	CCSGG	4 cut(s)	2745, 2746, 3053, 3054
BcoDI	GTCTC	8 cut(s)	215, 729, 1146, 1311, 1400, 3037, 3164, 3945
BfaI	CTAG	7 cut(s)	1013, 1301, 2112, 2216, 2379, 2447, 3230
BfmI	CTRYAG	6 cut(s)	223, 1068, 1121, 1435, 3021, 3573
BfoI	RGCGCY	1 cut(s)	1301
BfrI	CTTAAG	1 cut(s)	1834
BfuAI	ACCTGC	1 cut(s)	1967
BglII	AGATCT	2 cut(s)	2108, 2954
BisI	GCNGC	9 cut(s)	723, 876, 1245, 1287, 1722, 2031, 2639, 3794, 3810
BlpI	GCTNAGC	2 cut(s)	699, 2184
BlsI	GCNGC	9 cut(s)	724, 877, 1246, 1288, 1723, 2032, 2640, 3795, 3811
BmcAI	AGTACT	1 cut(s)	2228
Bme1390I	CCNGG	8 cut(s)	345, 1355, 1485, 1941, 2745, 2746, 3053, 3054
Bme18I	GGWCC	5 cut(s)	678, 1937, 1943, 2654, 3502
BmeRI	GACNNNNNGTC	1 cut(s)	2715
BmeT110I	CYCGRG	4 cut(s)	991, 2744, 3012, 3052
BmgT120I	GGNCC	5 cut(s)	678, 1937, 1943, 2654, 3502
BmiI	GGNNCC	4 cut(s)	1921, 2244, 2655, 3367
BmrFI	CCNGG	8 cut(s)	345, 1355, 1485, 1941, 2745, 2746, 3053, 3054
BmrI	ACTGGG	1 cut(s)	2683
BmsI	GCATC	8 cut(s)	469, 892, 1063, 1328, 2086, 2593, 2809, 3624
BmuI	ACTGGG	1 cut(s)	2683
BoxI	GACNNNNGTC	1 cut(s)	2625
BpiI	GAAGAC	3 cut(s)	1775, 2280, 2527
BpmI	CTGGAG	4 cut(s)	753, 1884, 2535, 2603
Bpu10I	CCTNAGC	1 cut(s)	2660
Bpu1102I	GCTNAGC	2 cut(s)	699, 2184
BpuEI	CTTGAG	4 cut(s)	92, 2289, 2633, 2948
BpuMI	CCSGG	4 cut(s)	2745, 2746, 3053, 3054
BsaAI	YACGTR	2 cut(s)	2320, 3209
BsaBI	GATNNNNATC	1 cut(s)	2557
BsaI	GGTCTC	2 cut(s)	3037, 3164
BsaWI	WCCGGW	2 cut(s)	826, 1144
BsaXI	ACNNNNNCTCC	6 cut(s)	864, 894, 3468, 3498, 3591, 3621
Bsc4I	CCNNNNNNNGG	5 cut(s)	991, 1904, 2497, 3053, 3375
Bse1I	ACTGG	6 cut(s)	463, 1867, 2150, 2586, 2678, 3751
Bse3DI	GCAATG	4 cut(s)	1268, 1684, 2877, 3797
Bse8I	GATNNNNATC	1 cut(s)	2557
BseAI	TCCGGA	1 cut(s)	826
BseBI	CCWGG	4 cut(s)	345, 1355, 1485, 1941
BseGI	GGATG	7 cut(s)	242, 286, 354, 2128, 2524, 2818, 3269
BseJI	GATNNNNATC	1 cut(s)	2557
BseLI	CCNNNNNNNGG	5 cut(s)	991, 1904, 2497, 3053, 3375
BseMI	GCAATG	4 cut(s)	1268, 1684, 2877, 3797
BseNI	ACTGG	6 cut(s)	463, 1867, 2150, 2586, 2678, 3751
BseRI	GAGGAG	2 cut(s)	2191, 3763
BseXI	GCAGC	6 cut(s)	734, 887, 1273, 1708, 2017, 3821
BsgI	GTGCAG	1 cut(s)	1995
Bsh1236I	CGCG	4 cut(s)	308, 1164, 2641, 3765
BshFI	GGCC	2 cut(s)	950, 1572
BsiHKAI	GWGCWC	1 cut(s)	2743
BsiHKCI	CYCGRG	4 cut(s)	991, 2744, 3012, 3052
BsiSI	CCGG	4 cut(s)	827, 1145, 2745, 3053
BslFI	GGGAC	3 cut(s)	453, 1466, 3698
BslI	CCNNNNNNNGG	5 cut(s)	991, 1904, 2497, 3053, 3375
BsmAI	GTCTC	8 cut(s)	215, 729, 1146, 1311, 1400, 3037, 3164, 3945
BsmFI	GGGAC	3 cut(s)	453, 1466, 3698
BsmI	GAATGC	3 cut(s)	898, 1793, 2349
BsnI	GGCC	2 cut(s)	950, 1572
Bso31I	GGTCTC	2 cut(s)	3037, 3164
BsoBI	CYCGRG	4 cut(s)	991, 2744, 3012, 3052
Bsp1286I	GDGCHC	1 cut(s)	2743
Bsp13I	TCCGGA	1 cut(s)	826
Bsp1407I	TGTACA	1 cut(s)	338
Bsp1720I	GCTNAGC	2 cut(s)	699, 2184
Bsp19I	CCATGG	1 cut(s)	3456
BspANI	GGCC	2 cut(s)	950, 1572
BspEI	TCCGGA	1 cut(s)	826
BspFNI	CGCG	4 cut(s)	308, 1164, 2641, 3765
BspHI	TCATGA	1 cut(s)	3627
BspLI	GGNNCC	4 cut(s)	1921, 2244, 2655, 3367
BspMAI	CTGCAG	1 cut(s)	3577
BspMI	ACCTGC	1 cut(s)	1967
BspPI	GGATC	8 cut(s)	47, 161, 666, 1386, 2882, 3232, 3348, 3528
BspTI	CTTAAG	1 cut(s)	1834
BspTNI	GGTCTC	2 cut(s)	3037, 3164
BsrDI	GCAATG	4 cut(s)	1268, 1684, 2877, 3797
BsrGI	TGTACA	1 cut(s)	338
BsrI	ACTGG	6 cut(s)	463, 1867, 2150, 2586, 2678, 3751
BssT1I	CCWWGG	2 cut(s)	3241, 3456
Bst2UI	CCWGG	4 cut(s)	345, 1355, 1485, 1941
Bst6I	CTCTTC	5 cut(s)	222, 1644, 2377, 2808, 3630
BstAFI	CTTAAG	1 cut(s)	1834
BstAPI	GCANNNNNTGC	1 cut(s)	2039
BstAUI	TGTACA	1 cut(s)	338
BstBAI	YACGTR	2 cut(s)	2320, 3209
BstC8I	GCNNGC	2 cut(s)	1065, 2440
BstDSI	CCRYGG	2 cut(s)	2639, 3456
BstF5I	GGATG	7 cut(s)	242, 286, 354, 2128, 2524, 2818, 3269
BstFNI	CGCG	4 cut(s)	308, 1164, 2641, 3765
BstH2I	RGCGCY	1 cut(s)	1301
BstHHI	GCGC	3 cut(s)	1300, 2444, 2453
BstMAI	GTCTC	8 cut(s)	215, 729, 1146, 1311, 1400, 3037, 3164, 3945
BstNI	CCWGG	4 cut(s)	345, 1355, 1485, 1941
BstNSI	RCATGY	3 cut(s)	2021, 2343, 2442
BstPAI	GACNNNNGTC	1 cut(s)	2625
BstSCI	CCNGG	8 cut(s)	343, 1353, 1483, 1939, 2743, 2744, 3051, 3052
BstSFI	CTRYAG	6 cut(s)	223, 1068, 1121, 1435, 3021, 3573
BstSNI	TACGTA	1 cut(s)	2320
BstUI	CGCG	4 cut(s)	308, 1164, 2641, 3765
BstV1I	GCAGC	6 cut(s)	734, 887, 1273, 1708, 2017, 3821
BstV2I	GAAGAC	3 cut(s)	1775, 2280, 2527
BstX2I	RGATCY	3 cut(s)	2108, 2954, 3533
BstXI	CCANNNNNNTGG	1 cut(s)	958
BstYI	RGATCY	3 cut(s)	2108, 2954, 3533
BsuRI	GGCC	2 cut(s)	950, 1572
BtgI	CCRYGG	2 cut(s)	2639, 3456
BtgZI	GCGATG	1 cut(s)	2747
BtsCI	GGATG	7 cut(s)	242, 286, 354, 2128, 2524, 2818, 3269
BtsI	GCAGTG	3 cut(s)	2829, 2903, 3252
BtsIMutI	CAGTG	8 cut(s)	1215, 1444, 1556, 2579, 2829, 2903, 3252, 3744
BveI	ACCTGC	1 cut(s)	1967
Cac8I	GCNNGC	2 cut(s)	1065, 2440
CaiI	CAGNNNCTG	1 cut(s)	3815
CciI	TCATGA	1 cut(s)	3627
CfoI	GCGC	3 cut(s)	1300, 2444, 2453
Cfr13I	GGNCC	5 cut(s)	678, 1937, 1943, 2654, 3502
Cfr42I	CCGCGG	1 cut(s)	2642
Cfr9I	CCCGGG	2 cut(s)	2744, 3052
CseI	GACGC	1 cut(s)	3771
CspCI	CAANNNNNGTGG	2 cut(s)	3107, 3142
DraI	TTTAAA	2 cut(s)	1173, 1687
DrdI	GACNNNNNNGTC	1 cut(s)	2282
DriI	GACNNNNNGTC	1 cut(s)	2715
DseDI	GACNNNNNNGTC	1 cut(s)	2282
EaeI	YGGCCR	2 cut(s)	948, 1570
Eam1104I	CTCTTC	5 cut(s)	222, 1644, 2377, 2808, 3630
Eam1105I	GACNNNNNGTC	1 cut(s)	2715
EarI	CTCTTC	5 cut(s)	222, 1644, 2377, 2808, 3630
Ecl136II	GAGCTC	1 cut(s)	2741
Eco105I	TACGTA	1 cut(s)	2320
Eco130I	CCWWGG	2 cut(s)	3241, 3456
Eco24I	GRGCYC	1 cut(s)	2743
Eco31I	GGTCTC	2 cut(s)	3037, 3164
Eco32I	GATATC	3 cut(s)	814, 1535, 2934
Eco47I	GGWCC	5 cut(s)	678, 1937, 1943, 2654, 3502
Eco53kI	GAGCTC	1 cut(s)	2741
Eco57I	CTGAAG	3 cut(s)	276, 438, 1825
Eco88I	CYCGRG	4 cut(s)	991, 2744, 3012, 3052
EcoICRI	GAGCTC	1 cut(s)	2741
EcoO109I	RGGNCCY	1 cut(s)	1943
EcoRII	CCWGG	4 cut(s)	343, 1353, 1483, 1939
EcoRV	GATATC	3 cut(s)	814, 1535, 2934
EcoT14I	CCWWGG	2 cut(s)	3241, 3456
EcoT22I	ATGCAT	1 cut(s)	1270
EcoT38I	GRGCYC	1 cut(s)	2743
ErhI	CCWWGG	2 cut(s)	3241, 3456
FalI	AAGNNNNNCTT	6 cut(s)	588, 620, 910, 942, 3489, 3521
FaqI	GGGAC	3 cut(s)	453, 1466, 3698
FauI	CCCGC	3 cut(s)	301, 323, 1367
FauNDI	CATATG	1 cut(s)	957
FbaI	TGATCA	1 cut(s)	840
Fnu4HI	GCNGC	9 cut(s)	723, 876, 1245, 1287, 1722, 2031, 2639, 3794, 3810
FokI	GGATG	7 cut(s)	249, 273, 361, 2115, 2511, 2825, 3256
FriOI	GRGCYC	1 cut(s)	2743
Fsp4HI	GCNGC	9 cut(s)	723, 876, 1245, 1287, 1722, 2031, 2639, 3794, 3810
FspAI	RTGCGCAY	1 cut(s)	2443
FspBI	CTAG	7 cut(s)	1013, 1301, 2112, 2216, 2379, 2447, 3230
FspI	TGCGCA	1 cut(s)	2443
GlaI	GCGC	3 cut(s)	1299, 2443, 2452
GluI	GCNGC	9 cut(s)	723, 876, 1245, 1287, 1722, 2031, 2639, 3794, 3810
GsuI	CTGGAG	4 cut(s)	753, 1884, 2535, 2603
HaeII	RGCGCY	1 cut(s)	1301
HaeIII	GGCC	2 cut(s)	950, 1572
HapII	CCGG	4 cut(s)	827, 1145, 2745, 3053
HgaI	GACGC	1 cut(s)	3771
HhaI	GCGC	3 cut(s)	1300, 2444, 2453
Hin6I	GCGC	3 cut(s)	1298, 2442, 2451
HinP1I	GCGC	3 cut(s)	1298, 2442, 2451
HincII	GTYRAC	1 cut(s)	3742
HindII	GTYRAC	1 cut(s)	3742
HindIII	AAGCTT	4 cut(s)	1519, 1731, 1831, 1837
HpaII	CCGG	4 cut(s)	827, 1145, 2745, 3053
HphI	GGTGA	8 cut(s)	1220, 1709, 1751, 2168, 2897, 2977, 3800, 3946
Hpy166II	GTNNAC	7 cut(s)	341, 1463, 3129, 3502, 3642, 3706, 3742
Hpy8I	GTNNAC	7 cut(s)	341, 1463, 3129, 3502, 3642, 3706, 3742
Hpy99I	CGWCG	1 cut(s)	3787
HpyCH4IV	ACGT	6 cut(s)	2319, 2364, 2467, 2622, 3208, 3729
HpySE526I	ACGT	6 cut(s)	2319, 2364, 2467, 2622, 3208, 3729
HspAI	GCGC	3 cut(s)	1298, 2442, 2451
Kpn2I	TCCGGA	1 cut(s)	826
Ksp22I	TGATCA	1 cut(s)	840
KspI	CCGCGG	1 cut(s)	2642
LmnI	GCTCC	9 cut(s)	872, 1204, 1718, 1790, 2242, 2584, 2678, 2746, 3599
Lsp1109I	GCAGC	6 cut(s)	734, 887, 1273, 1708, 2017, 3821
LweI	GCATC	8 cut(s)	469, 892, 1063, 1328, 2086, 2593, 2809, 3624
MaeI	CTAG	7 cut(s)	1013, 1301, 2112, 2216, 2379, 2447, 3230
MaeII	ACGT	6 cut(s)	2319, 2364, 2467, 2622, 3208, 3729
MfeI	CAATTG	1 cut(s)	2278
MflI	RGATCY	3 cut(s)	2108, 2954, 3533
MhlI	GDGCHC	1 cut(s)	2743
MlsI	TGGCCA	2 cut(s)	950, 1572
MluI	ACGCGT	1 cut(s)	1162
MluNI	TGGCCA	2 cut(s)	950, 1572
MlyI	GAGTC	5 cut(s)	731, 1148, 2379, 3492, 3571
MmeI	TCCRAC	5 cut(s)	656, 1510, 1696, 3079, 3460
Mox20I	TGGCCA	2 cut(s)	950, 1572
Mph1103I	ATGCAT	1 cut(s)	1270
MroI	TCCGGA	1 cut(s)	826
MscI	TGGCCA	2 cut(s)	950, 1572
MslI	CAYNNNNRTG	2 cut(s)	956, 2847
Msp20I	TGGCCA	2 cut(s)	950, 1572
MspA1I	CMGCKG	3 cut(s)	878, 2641, 2659
MspCI	CTTAAG	1 cut(s)	1834
MspI	CCGG	4 cut(s)	827, 1145, 2745, 3053
MspR9I	CCNGG	8 cut(s)	345, 1355, 1485, 1941, 2745, 2746, 3053, 3054
MunI	CAATTG	1 cut(s)	2278
Mva1269I	GAATGC	3 cut(s)	898, 1793, 2349
MvaI	CCWGG	4 cut(s)	345, 1355, 1485, 1941
MvnI	CGCG	4 cut(s)	308, 1164, 2641, 3765
NciI	CCSGG	4 cut(s)	2745, 2746, 3053, 3054
NcoI	CCATGG	1 cut(s)	3456
NdeI	CATATG	1 cut(s)	957
NlaIV	GGNNCC	4 cut(s)	1921, 2244, 2655, 3367
NmeAIII	GCCGAG	1 cut(s)	93
NmuCI	GTSAC	3 cut(s)	2220, 3370, 3508
NsbI	TGCGCA	1 cut(s)	2443
NsiI	ATGCAT	1 cut(s)	1270
NspI	RCATGY	3 cut(s)	2021, 2343, 2442
PaeI	GCATGC	1 cut(s)	2442
PaeR7I	CTCGAG	1 cut(s)	3012
PagI	TCATGA	1 cut(s)	3627
PciI	ACATGT	1 cut(s)	2339
PcsI	WCGNNNNNNNCGW	2 cut(s)	120, 1391
PctI	GAATGC	3 cut(s)	898, 1793, 2349
PfeI	GAWTC	8 cut(s)	33, 44, 292, 1183, 1473, 1592, 2774, 3893
PkrI	GCNGC	9 cut(s)	724, 877, 1246, 1288, 1723, 2032, 2640, 3795, 3811
PleI	GAGTC	5 cut(s)	731, 1148, 2378, 3491, 3570
PpsI	GAGTC	5 cut(s)	731, 1148, 2378, 3491, 3570
Ppu21I	YACGTR	2 cut(s)	2320, 3209
PpuMI	RGGWCCY	1 cut(s)	1943
PscI	ACATGT	1 cut(s)	2339
PshAI	GACNNNNGTC	1 cut(s)	2625
PshBI	ATTAAT	1 cut(s)	3414
PsiI	TTATAA	2 cut(s)	588, 2165
Psp124BI	GAGCTC	1 cut(s)	2743
Psp1406I	AACGTT	1 cut(s)	3729
Psp5II	RGGWCCY	1 cut(s)	1943
Psp6I	CCWGG	4 cut(s)	343, 1353, 1483, 1939
PspGI	CCWGG	4 cut(s)	343, 1353, 1483, 1939
PspN4I	GGNNCC	4 cut(s)	1921, 2244, 2655, 3367
PspPI	GGNCC	5 cut(s)	678, 1937, 1943, 2654, 3502
PspPPI	RGGWCCY	1 cut(s)	1943
PstI	CTGCAG	1 cut(s)	3577
PstNI	CAGNNNCTG	1 cut(s)	3815
PsuI	RGATCY	3 cut(s)	2108, 2954, 3533
PvuII	CAGCTG	1 cut(s)	878
RseI	CAYNNNNRTG	2 cut(s)	956, 2847
SacI	GAGCTC	1 cut(s)	2743
SacII	CCGCGG	1 cut(s)	2642
SatI	GCNGC	9 cut(s)	723, 876, 1245, 1287, 1722, 2031, 2639, 3794, 3810
Sau96I	GGNCC	5 cut(s)	678, 1937, 1943, 2654, 3502
ScaI	AGTACT	1 cut(s)	2228
SchI	GAGTC	5 cut(s)	731, 1148, 2379, 3492, 3571
ScrFI	CCNGG	8 cut(s)	345, 1355, 1485, 1941, 2745, 2746, 3053, 3054
SduI	GDGCHC	1 cut(s)	2743
SfaNI	GCATC	8 cut(s)	469, 892, 1063, 1328, 2086, 2593, 2809, 3624
SfcI	CTRYAG	6 cut(s)	223, 1068, 1121, 1435, 3021, 3573
Sfr274I	CTCGAG	1 cut(s)	3012
Sfr303I	CCGCGG	1 cut(s)	2642
SgrBI	CCGCGG	1 cut(s)	2642
SinI	GGWCC	5 cut(s)	678, 1937, 1943, 2654, 3502
SlaI	CTCGAG	1 cut(s)	3012
SmaI	CCCGGG	2 cut(s)	2746, 3054
SmiMI	CAYNNNNRTG	2 cut(s)	956, 2847
SmlI	CTYRAG	6 cut(s)	71, 1834, 2304, 2612, 2927, 3012
SmoI	CTYRAG	6 cut(s)	71, 1834, 2304, 2612, 2927, 3012
SnaBI	TACGTA	1 cut(s)	2320
SphI	GCATGC	1 cut(s)	2442
SspMI	CTAG	7 cut(s)	1013, 1301, 2112, 2216, 2379, 2447, 3230
SstI	GAGCTC	1 cut(s)	2743
StyD4I	CCNGG	8 cut(s)	343, 1353, 1483, 1939, 2743, 2744, 3051, 3052
StyI	CCWWGG	2 cut(s)	3241, 3456
TaiI	ACGT	6 cut(s)	2322, 2367, 2470, 2625, 3211, 3732
TaqII	GACCGA	2 cut(s)	3061, 3608
TatI	WGTACW	6 cut(s)	338, 455, 2226, 3136, 3428, 3639
TauI	GCSGC	3 cut(s)	1247, 2641, 3796
TfiI	GAWTC	8 cut(s)	33, 44, 292, 1183, 1473, 1592, 2774, 3893
TscAI	CASTG	8 cut(s)	1222, 1444, 1563, 2586, 2829, 2910, 3259, 3751
TseFI	GTSAC	3 cut(s)	2220, 3370, 3508
TseI	GCWGC	6 cut(s)	722, 875, 1286, 1721, 2030, 3809
Tsp45I	GTSAC	3 cut(s)	2220, 3370, 3508
TspGWI	ACGGA	2 cut(s)	2513, 3358
TspMI	CCCGGG	2 cut(s)	2744, 3052
TspRI	CASTG	8 cut(s)	1222, 1444, 1563, 2586, 2829, 2910, 3259, 3751
Vha464I	CTTAAG	1 cut(s)	1834
VpaK11BI	GGWCC	5 cut(s)	678, 1937, 1943, 2654, 3502
VspI	ATTAAT	1 cut(s)	3414
XapI	RAATTY	7 cut(s)	529, 574, 1220, 1682, 2065, 2864, 3899
XceI	RCATGY	3 cut(s)	2021, 2343, 2442
XhoI	CTCGAG	1 cut(s)	3012
XmaI	CCCGGG	2 cut(s)	2744, 3052
XspI	CTAG	7 cut(s)	1013, 1301, 2112, 2216, 2379, 2447, 3230
ZrmI	AGTACT	1 cut(s)	2228
Zsp2I	ATGCAT	1 cut(s)	1270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.