Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003184

Physical Location & Seq

Genomic Coordinates Reverse (-)

21372145 .. 21386878

Gene Structure 14734 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1178400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4098 bp

ATGGCCGACGAAAGAGACGCAGCAGCAGGAAGCGGTGGCAGCCTGGTTTTTGCAGTTAACGGAGAGAGGTTTGAGCTCCCGACCGTTGATCCTTCAACCACTCTGCTCGCCTTCTTGCGTTCTCAGACGAGATTCAAAAGTGTCAAGCTCAGCTGCGGTGAAGGTGGTTGCGGTGCTTGTGTTGTGCTACTGTCCAAGTACGACCCTGTTGTCGACGAGGTGAAAGATTTTACTGCAAGTTCATGTCTCACTCTGCTTTGTAGTATAAATGGATGTTCAATTACAACATCCGAAGGCCTTGGAAATATCAAAGACGGGTTCCACCCAATTCACCAAAGGATTGCTGGTTTCCATGCTTCTCAATGCGGCTTTTGTACTCCTGGCATGTGTGTTTCACTCTTTGGAGCTCTTGTCAATGCTGAAAAGGCGAATAGACCTGATCCCCCTCCTGGGTTCTCCAAGCTTTCTGTTTCCGAAGCTGAAAAGTCTATTGCAGGTAACCTCTGTCGATGTACGGGGTATCGACCAATTGCTGATGCCTGCAAGAGTTTCGCAGCTGATGTTGATATGGAGGATTTGGGGTTCAACTCCTTTTGGAAAAAGGGAGATAGTAAGGAAGTAAAAGTAAAGAGTTTGCCTCTATATAACCGTAATGGTGACATCTGTACATTTCCGGACTTCTTAAAAAAAGAAATTTGTTCTTCCATGTCTTTGGATCCTACGAGATATGGTTGGTACAATCCTGTTAGAGTTGAGGAGCTTCAAAACTTATTGAAAGATAATGATTTTGATAATGCAAACGAGATGAAATTAGTTGTTGGAAACACAGGGACAGGCTATTACAAGGAGCTAAAGCGCTATGACAGATACATTGATCTCAGATGTGTTCCTGAATTATCAATGGTTAAGAAGGATCCTGTGGGGGTTCAGTTCGGAGCAACTGTAACAATCTCTAAAGTTATTGAGTCTTTGAGGAAGAAAGACAACAGTGGATCTCCATCAAGAGGTGGTGATGTGTTAAAAAAAATTGCCAATCATATGGAGAAAATTGCTTCAGGGTTCATCAGAAATACAGGTAGTATAGGAGGGAATTTGGTGATGGCACAGAGGAAATGTTTTCCTTCAGATATTGCTACAATACTTCTTGCTGTGGATTCAGAGGTAGATATAATGAATGGTGCTAGATCTGAAACGGTTATGTTAGAGGATTTTCTGAAAAGGCCTCCATTGGATCCGAAAAGTGTACTTTTAAGTGTTAAAATCCCAAATTGGGAAGCAGTTAGAAAAGTTTCTCCAGAAACCAACACCATGTTGCTTTTTGAAACCTATCGAGCTGCACCAAGACCCCTAGGAAGGGCATTAGCTTATTTAAATTCTGCCTTCCTAGCAGAGGTTTCGTTTGGTAAGATTTCCAATGGAATTATGGTGGATCATTGTCGTCTGGCTTTTGGTGCTTATGGAACTAAACATGCAATTAGAGCAAGAAAGGTTGAGGAATTTTTAACTGGAAAAGTGCTGAGTCCTGGTGTTCTATATGACGCTATTAAATTAGTTAGGGATGTTGTGGTTCCTGAAGAAGGAACTACGAGCCCTGCTTATAGGTCAAGCATGGCTGCTGGTTTTCTTTTCGAGTTCTTTAGCCCCTTAATTGACAGTGAAAGTTCCAATGGATTCTTAGGAAATACTTTGTTGGCCGATTCTTCCAAGTTAAAAAGGAACAAAAGTGCTAGCGATAAGATGAGAACTGTTCTGTCATCTGGTAAACAGGTGATTGAATTAAGTACAGAGTATGATCCGGTTGGTAAGCCAATTACAAAATCTGGAGGTCTCATACAAGCTTCAGGTGAGGCAGTCTATGTAGACGACATTCCATCTCCGAAAAATTGCTTACATGGAGCGTTCATTTATAGTACAAAGCCTTTGGCTAGAGTGAAGGAAATAAAATTTGAACCTAAACCACAGCCAGGAGTTACTGCACTGATATCTTTCAAAGATATTCCCAAGTCTGGGGAGAACATAGGGTCTAAGACTTTTCTTGGTACAGAACCTCTGTTTGCTAATGATTTTACAGAATGGGCTGGTCAGCGTCTTGCTTTCGTGGTAGCAGATACGCAGAAACATGCGGATATGGCTACAAACTTTGCGGTGGTTGATTATAACATGGAAGATGTAGATCCACCTATCTTATCAGTCGAAGAGGCAGTTAAGAGAGCGAGTTTTTTTGAGGTCCCTCCTTTCTTGTATCCGAAACAGGTTGGTGACATATCAAATGGAATGGCTGCAGCTGATCACAAGATTATTTCTGCTGAGATCAAACTTGGTTCACAATACTATTTCTACATGGAGACACAAACAGCCCTTGCTGTACCAGATGAAGACAACTGCATGGTGGTTTACACTTCAACTCAAGTGCCTGAGTATACACACTCTACAATTGCAAAATGTCTTGGTATTCCTGAAAATAATGTTCGTGTAATTACAAGAAGGGTCGGAGGGGGCTTTGGTGGAAAGGCCATGCTATCAATGCCTGTCGCAACTGCATGTGCTCTTGCAGCACACAAATTACATCGACCTGTACGGATATATCTCAATCGCAAGACTGATATGATAATGGCTGGAGGAAGGCATCCCATGAAGATAACTTACAGTGTGGGATTCAAGTCTGATGGGAAGATTACAGCTTTAGAACTTGAGATATTGATAAATGCAGGGATTTCCATAGACTTTAGTCCATTATTGCCAAAAAATATTGTGACTGCACTTAAGAAGTATGACTGGGGTGCTCTGGCTTTCGATATCAAGGTGTGCAAAACAAACACTCCAAGTAGGTCTGCTATGCGGGCCCCTGGGGAGGTGCAGGGGTCATTTATCGCTGAAGCTGTAATTGAACATGTAGCATCCACCCTTTCTATGGAAGTAGATTCTGTGAGAAGCGTTAATCTTCACACATCTCACAGCCTTGATTTGTTTTACGAGCAGAGCGCTGGTGAACCCCTGGAGTATACTTTACCATTGATATGGAACAAGTTGGCCATGTCTTCAAGCTTTAACCAAAGGACTGAGATGGTAAAAGAATTTAATAGGTGTAATAAATGGCAGAAAAGAGGAATTTCTCGAGTACCGATAGTGTATGAAGTGTCATTGAGGCCAACTCCGGGAAAAGTAAGCATTTTAAGTGATGGGTCTGTTGTTGTTGAAGTTGGAGGAATTGAACTGGGTCAGGGGCTCTGGACGAAGGTAAAACAGGTGACAGCTTTTGCTCTTGGTTCGATTCAATGTGATGGTAGTGGAGATTTCTTGGATAAAATACGGGTGGTGCAATCAGATACATTGAGCTTAATTCAAGGGGGAATGACTGCTGGGAGCACTACATCTGAGACAAGCTGTGAGGCAGTTAGACTTTGCTGTAATATTTTGGTTGAGAGGTTAGCTACTCTCAAGGGGAGATTGAAGGAGCAAATGGGCTCTATTAACTGGGAGACTCTTATTCAGCAGGCATCCTTGCAAGCTGTGAACTTATCAGCAAGTTCTTATTATGTCCCTGATTCTGCTTCCATGGCATACCTTAACTATGGTGCTGCTGTGAGTGAGGTGGAGGTAAATGTTCTGACTGGACAAACCACAATTCTGCAATCAGATATTATCTACGATTGTGGTCAGAGTCTCAACCCTGCTGTGGACTTAGGACAGATCGAAGGTTCTTTTGTTCAAGGTATTGGGTTTTTCATGCTTGAAGAATACTTGGAAAACTCTGATGGGTTGGTGATTTCAGAAGGTACATGGACATATAAGATTCCTACGGTGGACACCATACCCAAACAGTTCAACGTTCAAGTATTGAACAGTGGGCATCATAAAAAACACGTGCTCTCTTCAAAAGCTTCTGGTGAGCCACCATTACTTCTTGCGGTTTCTGTTCACTGCGCTACAAGGGCTGCCATTAAAGAATCAAGAAAACAGCTTCTTCAGTGGGGCGGCCTAGATGGATCGGCATCAATATTCCAATTAGATGTTCCCGCTACCATGCCTGTTGTGAAGGAGCTGTGCGGACTGGAAGCTGTGGAGAGGTACTTGGAATGGATTGTAGGCAGAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004029 GO:0004031 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009687 GO:0009688 GO:0009850 GO:0009851 GO:0009987 GO:0010293 GO:0010817 GO:0016053 GO:0016106 GO:0016114 GO:0016143 GO:0016491 GO:0016620 GO:0016623 GO:0016903 GO:0018479 GO:0018488 GO:0019748 GO:0019752 GO:0019757 GO:0019760 GO:0019899 GO:0031625 GO:0032787 GO:0036094 GO:0042445 GO:0042446 GO:0043167 GO:0043168 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0046165 GO:0046394 GO:0048037 GO:0050302 GO:0050660 GO:0050662 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0097159 GO:1901135 GO:1901265 GO:1901363 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901657 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00380 ko00906 ko01100 ko01110 map00380 map00906 map01100 map01110

Pfam Domains

Protein Families

Ald_Xan_dh_C Ald_Xan_dh_C2 CO_deh_flav_C FAD_binding_5 Fer2 Fer2_2

Protein Analysis

1366

Amino Acids

148.66

Weight (kDa)

6.44

Isoelectric Point (pI)

34.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	18 - 84	8.8e-08	2Fe-2S iron-sulfur cluster binding domain
Fer2_2	PF01799	96 - 182	6.8e-21	[2Fe-2S] binding domain
FAD_binding_5	PF00941	242 - 424	1.2e-39	FAD binding domain in molybdopterin dehydrogenase
CO_deh_flav_C	PF03450	442 - 547	2.2e-18	CO dehydrogenase flavoprotein C-terminal domain
Ald_Xan_dh_C	PF01315	614 - 721	6.1e-28	Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead domain
MoCoBD_1	PF02738	750 - 981	2.4e-84	Molybdopterin cofactor-binding domain
MoCoBD_2	PF20256	1010 - 1272	1.4e-72	Molybdopterin cofactor-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04580 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT2G27150 AT3G43600
fragaria_vesca	FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980 FvH4_3g26980
malus_domestica	MD00G1162900.v1.1 MD03G1162200.v1.1 MD11G1178400.v1.1 MD11G1178500.v1.1
prunus_persica	Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1 Prupe.6G150900_v2.0.a1
pyrus_communis	pycom03g11520 pycom11g15240 pycom11g15280 pycom11g15360
rosa_chinensis	RchiOBHm_Chr5g0049511
rosa_laevigata	RLG00000034622
rosa_multiflora	Rmu_sc0001307.1_g000031
rosa_roxburghii	Rroxscaffold_1G00032200
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh5AG325300 Rh5BG336500 Rh5CG361200 Rh5DG348600
rosa_wichuraiana	Rw5G030710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2157
AasI	GACNNNNNNGTC	1 cut(s)	209
Acc36I	ACCTGC	1 cut(s)	485
AccI	GTMKAC	4 cut(s)	213, 1860, 2420, 3002
AccIII	TCCGGA	1 cut(s)	673
AclI	AACGTT	1 cut(s)	3828
AcoI	YGGCCR	3 cut(s)	3, 1692, 3030
AcsI	RAATTY	7 cut(s)	693, 1090, 1372, 1496, 1943, 3074, 3108
AcuI	CTGAAG	6 cut(s)	1038, 1107, 1593, 1824, 2895, 3950
AcvI	CACGTG	1 cut(s)	3865
AfeI	AGCGCT	2 cut(s)	857, 2983
AflII	CTTAAG	1 cut(s)	2762
AflIII	ACRYGT	2 cut(s)	2890, 3862
AjnI	CCWGG	7 cut(s)	42, 379, 448, 1522, 1963, 2845, 2994
AjuI	GAANNNNNNNTTGG	2 cut(s)	3996, 4028
AloI	GAACNNNNNNTCC	2 cut(s)	566, 598
Alw21I	GWGCWC	6 cut(s)	78, 409, 2548, 2785, 3368, 3870
Alw26I	GTCTC	7 cut(s)	9, 251, 1832, 2339, 3371, 3473, 3668
Ama87I	CYCGRG	1 cut(s)	3114
Aor13HI	TCCGGA	1 cut(s)	673
Aor51HI	AGCGCT	2 cut(s)	857, 2983
AoxI	GGCC	9 cut(s)	3, 295, 1220, 1692, 2511, 2841, 3030, 3146, 3976
ApaI	GGGCCC	1 cut(s)	2845
ApoI	RAATTY	7 cut(s)	693, 1090, 1372, 1496, 1943, 3074, 3108
Asp700I	GAANNNNTTC	1 cut(s)	1288
AspA2I	CCTAGG	1 cut(s)	1348
AspLEI	GCGC	3 cut(s)	858, 2984, 3926
AspS9I	GGNCC	3 cut(s)	2227, 2841, 2842
AsuC2I	CCSGG	1 cut(s)	3156
AsuNHI	GCTAGC	1 cut(s)	1727
AvaI	CYCGRG	1 cut(s)	3114
AvaII	GGWCC	1 cut(s)	2227
AvrII	CCTAGG	1 cut(s)	1348
BaeGI	GKGCMC	1 cut(s)	2845
BalI	TGGCCA	1 cut(s)	3032
BamHI	GGATCC	3 cut(s)	715, 913, 1231
BanII	GRGCYC	6 cut(s)	78, 409, 1592, 2845, 3228, 3467
BarI	GAAGNNNNNNTAC	2 cut(s)	2627, 2659
BbrPI	CACGTG	1 cut(s)	3865
BbsI	GAAGAC	2 cut(s)	2382, 3030
Bbv12I	GWGCWC	6 cut(s)	78, 409, 2548, 2785, 3368, 3870
BccI	CCATC	9 cut(s)	1006, 1093, 1879, 2660, 3058, 3173, 3275, 3749, 3977
BcgI	CGANNNNNNTGC	4 cut(s)	532, 566, 1377, 1411
BciT130I	CCWGG	7 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996
BciVI	GTATCC	1 cut(s)	2253
BclI	TGATCA	1 cut(s)	2287
BcnI	CCSGG	1 cut(s)	3156
BcoDI	GTCTC	7 cut(s)	9, 251, 1832, 2339, 3371, 3473, 3668
BfaI	CTAG	6 cut(s)	1182, 1349, 1385, 1728, 1926, 3980
BfmI	CTRYAG	1 cut(s)	2280
BfoI	RGCGCY	2 cut(s)	859, 2985
BfrI	CTTAAG	1 cut(s)	2762
BfuAI	ACCTGC	1 cut(s)	485
BfuI	GTATCC	1 cut(s)	2253
BglII	AGATCT	1 cut(s)	1184
BlnI	CCTAGG	1 cut(s)	1348
BlpI	GCTNAGC	1 cut(s)	149
Bme1390I	CCNGG	8 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996, 3156
Bme18I	GGWCC	1 cut(s)	2227
BmeT110I	CYCGRG	1 cut(s)	3114
BmgT120I	GGNCC	3 cut(s)	2227, 2841, 2842
BmiI	GGNNCC	8 cut(s)	320, 717, 915, 1233, 1569, 2229, 2843, 2844
BmrFI	CCNGG	8 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996, 3156
BmrI	ACTGGG	3 cut(s)	2785, 3224, 3484
BmsI	GCATC	6 cut(s)	526, 2635, 2906, 3506, 3859, 4001
BmtI	GCTAGC	1 cut(s)	1731
BmuI	ACTGGG	3 cut(s)	2785, 3224, 3484
BoxI	GACNNNNGTC	1 cut(s)	2727
BpiI	GAAGAC	2 cut(s)	2382, 3030
BplI	GAGNNNNNCTC	4 cut(s)	622, 654, 3136, 3168
BpmI	CTGGAG	4 cut(s)	1278, 1842, 2637, 3017
Bpu1102I	GCTNAGC	1 cut(s)	149
BpuEI	CTTGAG	3 cut(s)	2391, 2711, 3422
BpuMI	CCSGG	1 cut(s)	3156
BsaAI	YACGTR	1 cut(s)	3865
BsaBI	GATNNNNATC	3 cut(s)	997, 2172, 3991
BsaI	GGTCTC	1 cut(s)	1832
BsaJI	CCNNGG	7 cut(s)	298, 449, 1348, 2845, 2846, 2994, 3555
BsaWI	WCCGGW	2 cut(s)	673, 1795
BsaXI	ACNNNNNCTCC	6 cut(s)	396, 426, 927, 957, 2803, 2833
Bse1I	ACTGG	6 cut(s)	1510, 2780, 3219, 3479, 3616, 4056
Bse8I	GATNNNNATC	3 cut(s)	997, 2172, 3991
BseAI	TCCGGA	1 cut(s)	673
BseBI	CCWGG	7 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996
BseDI	CCNNGG	7 cut(s)	298, 449, 1348, 2845, 2846, 2994, 3555
BseGI	GGATG	6 cut(s)	278, 287, 1564, 2626, 2897, 3497
BseJI	GATNNNNATC	3 cut(s)	997, 2172, 3991
BseMII	CTCAG	8 cut(s)	137, 163, 892, 1508, 2298, 2406, 3051, 3366
BseNI	ACTGG	6 cut(s)	1510, 2780, 3219, 3479, 3616, 4056
BseRI	GAGGAG	1 cut(s)	770
BseSI	GKGCMC	1 cut(s)	2845
BseYI	CCCAGC	1 cut(s)	3359
BsgI	GTGCAG	4 cut(s)	1320, 1959, 2742, 2876
Bsh1285I	CGRYCG	1 cut(s)	84
BshFI	GGCC	9 cut(s)	5, 297, 1222, 1694, 2513, 2843, 3032, 3148, 3978
BsiEI	CGRYCG	1 cut(s)	84
BsiHKAI	GWGCWC	6 cut(s)	78, 409, 2548, 2785, 3368, 3870
BsiHKCI	CYCGRG	1 cut(s)	3114
BsiSI	CCGG	3 cut(s)	674, 1796, 3155
BslFI	GGGAC	3 cut(s)	844, 2213, 3524
BsmAI	GTCTC	7 cut(s)	9, 251, 1832, 2339, 3371, 3473, 3668
BsmBI	CGTCTC	1 cut(s)	9
BsmFI	GGGAC	3 cut(s)	844, 2213, 3524
BsnI	GGCC	9 cut(s)	5, 297, 1222, 1694, 2513, 2843, 3032, 3148, 3978
Bso31I	GGTCTC	1 cut(s)	1832
BsoBI	CYCGRG	1 cut(s)	3114
Bsp120I	GGGCCC	1 cut(s)	2841
Bsp13I	TCCGGA	1 cut(s)	673
Bsp1407I	TGTACA	1 cut(s)	665
Bsp1720I	GCTNAGC	1 cut(s)	149
Bsp19I	CCATGG	1 cut(s)	3555
BspANI	GGCC	9 cut(s)	5, 297, 1222, 1694, 2513, 2843, 3032, 3148, 3978
BspCNI	CTCAG	8 cut(s)	136, 162, 891, 1509, 2299, 2407, 3052, 3367
BspEI	TCCGGA	1 cut(s)	673
BspLI	GGNNCC	8 cut(s)	320, 717, 915, 1233, 1569, 2229, 2843, 2844
BspMAI	CTGCAG	1 cut(s)	2284
BspMI	ACCTGC	1 cut(s)	485
BspOI	GCTAGC	1 cut(s)	1731
BspTI	CTTAAG	1 cut(s)	2762
BspTNI	GGTCTC	1 cut(s)	1832
BsrGI	TGTACA	1 cut(s)	665
BsrI	ACTGG	6 cut(s)	1510, 2780, 3219, 3479, 3616, 4056
BssECI	CCNNGG	7 cut(s)	298, 449, 1348, 2845, 2846, 2994, 3555
BssNAI	GTATAC	2 cut(s)	2421, 3003
BssT1I	CCWWGG	3 cut(s)	298, 1348, 3555
Bst1107I	GTATAC	2 cut(s)	2421, 3003
Bst2UI	CCWGG	7 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996
Bst6I	CTCTTC	2 cut(s)	2190, 3877
BstAFI	CTTAAG	1 cut(s)	2762
BstAUI	TGTACA	1 cut(s)	665
BstBAI	YACGTR	1 cut(s)	3865
BstC8I	GCNNGC	6 cut(s)	108, 541, 1729, 2841, 3495, 3507
BstDSI	CCRYGG	1 cut(s)	3555
BstEII	GGTNACC	1 cut(s)	497
BstENI	CCTNNNNNAGG	2 cut(s)	1388, 1575
BstF5I	GGATG	6 cut(s)	278, 287, 1564, 2626, 2897, 3497
BstH2I	RGCGCY	2 cut(s)	859, 2985
BstHHI	GCGC	3 cut(s)	858, 2984, 3926
BstMAI	GTCTC	7 cut(s)	9, 251, 1832, 2339, 3371, 3473, 3668
BstMCI	CGRYCG	1 cut(s)	84
BstNI	CCWGG	7 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996
BstNSI	RCATGY	5 cut(s)	388, 1472, 2123, 2544, 2894
BstPAI	GACNNNNGTC	1 cut(s)	2727
BstPI	GGTNACC	1 cut(s)	497
BstSCI	CCNGG	8 cut(s)	42, 379, 448, 1522, 1963, 2845, 2994, 3154
BstSFI	CTRYAG	1 cut(s)	2280
BstSLI	GKGCMC	1 cut(s)	2845
BstV2I	GAAGAC	2 cut(s)	2382, 3030
BstX2I	RGATCY	6 cut(s)	715, 913, 992, 1184, 1231, 2173
BstXI	CCANNNNNNTGG	3 cut(s)	712, 1039, 3018
BstYI	RGATCY	6 cut(s)	715, 913, 992, 1184, 1231, 2173
BstZ17I	GTATAC	2 cut(s)	2421, 3003
BsuI	GTATCC	1 cut(s)	2253
BsuRI	GGCC	9 cut(s)	5, 297, 1222, 1694, 2513, 2843, 3032, 3148, 3978
BtgI	CCRYGG	1 cut(s)	3555
BtsCI	GGATG	6 cut(s)	278, 287, 1564, 2626, 2897, 3497
BtsI	GCAGTG	1 cut(s)	3919
BtsIMutI	CAGTG	7 cut(s)	994, 1660, 1976, 2653, 3850, 3919, 3974
BveI	ACCTGC	1 cut(s)	485
Cac8I	GCNNGC	6 cut(s)	108, 541, 1729, 2841, 3495, 3507
CfoI	GCGC	3 cut(s)	858, 2984, 3926
Cfr13I	GGNCC	3 cut(s)	2227, 2841, 2842
CseI	GACGC	3 cut(s)	26, 1547, 2075
DraI	TTTAAA	1 cut(s)	1371
DrdI	GACNNNNNNGTC	1 cut(s)	209
DseDI	GACNNNNNNGTC	1 cut(s)	209
EaeI	YGGCCR	3 cut(s)	3, 1692, 3030
Eam1104I	CTCTTC	2 cut(s)	2190, 3877
EarI	CTCTTC	2 cut(s)	2190, 3877
Ecl136II	GAGCTC	2 cut(s)	76, 407
Eco130I	CCWWGG	3 cut(s)	298, 1348, 3555
Eco147I	AGGCCT	2 cut(s)	297, 1222
Eco24I	GRGCYC	6 cut(s)	78, 409, 1592, 2845, 3228, 3467
Eco31I	GGTCTC	1 cut(s)	1832
Eco32I	GATATC	2 cut(s)	1983, 2797
Eco47I	GGWCC	1 cut(s)	2227
Eco47III	AGCGCT	2 cut(s)	857, 2983
Eco53kI	GAGCTC	2 cut(s)	76, 407
Eco57I	CTGAAG	6 cut(s)	1038, 1107, 1593, 1824, 2895, 3950
Eco72I	CACGTG	1 cut(s)	3865
Eco88I	CYCGRG	1 cut(s)	3114
Eco91I	GGTNACC	1 cut(s)	497
EcoICRI	GAGCTC	2 cut(s)	76, 407
EcoNI	CCTNNNNNAGG	2 cut(s)	1388, 1575
EcoO109I	RGGNCCY	2 cut(s)	2227, 2842
EcoO65I	GGTNACC	1 cut(s)	497
EcoRII	CCWGG	7 cut(s)	42, 379, 448, 1522, 1963, 2845, 2994
EcoRV	GATATC	2 cut(s)	1983, 2797
EcoT14I	CCWWGG	3 cut(s)	298, 1348, 3555
EcoT38I	GRGCYC	6 cut(s)	78, 409, 1592, 2845, 3228, 3467
ErhI	CCWWGG	3 cut(s)	298, 1348, 3555
Esp3I	CGTCTC	1 cut(s)	9
FaqI	GGGAC	3 cut(s)	844, 2213, 3524
FauI	CCCGC	2 cut(s)	2832, 4024
FauNDI	CATATG	1 cut(s)	1038
FbaI	TGATCA	1 cut(s)	2287
FblI	GTMKAC	4 cut(s)	213, 1860, 2420, 3002
FokI	GGATG	6 cut(s)	274, 285, 1571, 2613, 2884, 3484
FriOI	GRGCYC	6 cut(s)	78, 409, 1592, 2845, 3228, 3467
FspBI	CTAG	6 cut(s)	1182, 1349, 1385, 1728, 1926, 3980
GlaI	GCGC	3 cut(s)	857, 2983, 3925
GsaI	CCCAGC	1 cut(s)	3363
GsuI	CTGGAG	4 cut(s)	1278, 1842, 2637, 3017
HaeII	RGCGCY	2 cut(s)	859, 2985
HaeIII	GGCC	9 cut(s)	5, 297, 1222, 1694, 2513, 2843, 3032, 3148, 3978
HapII	CCGG	3 cut(s)	674, 1796, 3155
HgaI	GACGC	3 cut(s)	26, 1547, 2075
HhaI	GCGC	3 cut(s)	858, 2984, 3926
Hin6I	GCGC	3 cut(s)	856, 2982, 3924
HinP1I	GCGC	3 cut(s)	856, 2982, 3924
HincII	GTYRAC	2 cut(s)	58, 214
HindII	GTYRAC	2 cut(s)	58, 214
HindIII	AAGCTT	4 cut(s)	461, 1836, 3043, 3879
HpaI	GTTAAC	1 cut(s)	58
HpaII	CCGG	3 cut(s)	674, 1796, 3155
Hpy99I	CGWCG	2 cut(s)	11, 218
HpyCH4IV	ACGT	2 cut(s)	3828, 3864
HpySE526I	ACGT	2 cut(s)	3828, 3864
HspAI	GCGC	3 cut(s)	856, 2982, 3924
Kpn2I	TCCGGA	1 cut(s)	673
Ksp22I	TGATCA	1 cut(s)	2287
KspAI	GTTAAC	1 cut(s)	58
LmnI	GCTCC	9 cut(s)	81, 404, 757, 847, 935, 1895, 3363, 3454, 4039
LweI	GCATC	6 cut(s)	526, 2635, 2906, 3506, 3859, 4001
MaeI	CTAG	6 cut(s)	1182, 1349, 1385, 1728, 1926, 3980
MaeII	ACGT	2 cut(s)	3828, 3864
MaeIII	GTNAC	7 cut(s)	497, 656, 943, 1969, 2258, 2752, 3247
MfeI	CAATTG	2 cut(s)	528, 2433
MflI	RGATCY	6 cut(s)	715, 913, 992, 1184, 1231, 2173
MlsI	TGGCCA	1 cut(s)	3032
MluNI	TGGCCA	1 cut(s)	3032
MlyI	GAGTC	4 cut(s)	974, 1528, 3475, 3670
MmeI	TCCRAC	3 cut(s)	799, 2470, 3181
Mox20I	TGGCCA	1 cut(s)	3032
MroI	TCCGGA	1 cut(s)	673
MroXI	GAANNNNTTC	1 cut(s)	1288
MscI	TGGCCA	1 cut(s)	3032
MslI	CAYNNNNRTG	1 cut(s)	3016
Msp20I	TGGCCA	1 cut(s)	3032
MspA1I	CMGCKG	3 cut(s)	153, 557, 2285
MspCI	CTTAAG	1 cut(s)	2762
MspI	CCGG	3 cut(s)	674, 1796, 3155
MspR9I	CCNGG	8 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996, 3156
MunI	CAATTG	2 cut(s)	528, 2433
MvaI	CCWGG	7 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996
NciI	CCSGG	1 cut(s)	3156
NcoI	CCATGG	1 cut(s)	3555
NdeI	CATATG	1 cut(s)	1038
NheI	GCTAGC	1 cut(s)	1727
NlaIV	GGNNCC	8 cut(s)	320, 717, 915, 1233, 1569, 2229, 2843, 2844
NmuCI	GTSAC	4 cut(s)	656, 2258, 2752, 3247
NspI	RCATGY	5 cut(s)	388, 1472, 2123, 2544, 2894
PaeR7I	CTCGAG	1 cut(s)	3114
PasI	CCCWGGG	1 cut(s)	2846
PceI	AGGCCT	2 cut(s)	297, 1222
PciI	ACATGT	1 cut(s)	2890
PdmI	GAANNNNTTC	1 cut(s)	1288
PfoI	TCCNGGA	1 cut(s)	3154
PleI	GAGTC	4 cut(s)	973, 1527, 3475, 3669
PmaCI	CACGTG	1 cut(s)	3865
PmlI	CACGTG	1 cut(s)	3865
PpsI	GAGTC	4 cut(s)	973, 1527, 3475, 3669
Ppu21I	YACGTR	1 cut(s)	3865
PpuMI	RGGWCCY	1 cut(s)	2227
PscI	ACATGT	1 cut(s)	2890
PshAI	GACNNNNGTC	1 cut(s)	2727
PsiI	TTATAA	1 cut(s)	2157
Psp124BI	GAGCTC	2 cut(s)	78, 409
Psp1406I	AACGTT	1 cut(s)	3828
Psp5II	RGGWCCY	1 cut(s)	2227
Psp6I	CCWGG	7 cut(s)	42, 379, 448, 1522, 1963, 2845, 2994
PspCI	CACGTG	1 cut(s)	3865
PspEI	GGTNACC	1 cut(s)	497
PspFI	CCCAGC	1 cut(s)	3359
PspGI	CCWGG	7 cut(s)	42, 379, 448, 1522, 1963, 2845, 2994
PspN4I	GGNNCC	8 cut(s)	320, 717, 915, 1233, 1569, 2229, 2843, 2844
PspOMI	GGGCCC	1 cut(s)	2841
PspPI	GGNCC	3 cut(s)	2227, 2841, 2842
PspPPI	RGGWCCY	1 cut(s)	2227
PstI	CTGCAG	1 cut(s)	2284
PsuI	RGATCY	6 cut(s)	715, 913, 992, 1184, 1231, 2173
PvuII	CAGCTG	3 cut(s)	153, 557, 2285
RseI	CAYNNNNRTG	1 cut(s)	3016
SacI	GAGCTC	2 cut(s)	78, 409
SalI	GTCGAC	1 cut(s)	212
Sau96I	GGNCC	3 cut(s)	2227, 2841, 2842
SchI	GAGTC	4 cut(s)	974, 1528, 3475, 3670
ScrFI	CCNGG	8 cut(s)	44, 381, 450, 1524, 1965, 2847, 2996, 3156
SfaNI	GCATC	6 cut(s)	526, 2635, 2906, 3506, 3859, 4001
SfcI	CTRYAG	1 cut(s)	2280
Sfr274I	CTCGAG	1 cut(s)	3114
SinI	GGWCC	1 cut(s)	2227
SlaI	CTCGAG	1 cut(s)	3114
SmiI	ATTTAAAT	1 cut(s)	1371
SmiMI	CAYNNNNRTG	1 cut(s)	3016
SmlI	CTYRAG	5 cut(s)	2406, 2690, 2762, 3114, 3437
SmoI	CTYRAG	5 cut(s)	2406, 2690, 2762, 3114, 3437
SseBI	AGGCCT	2 cut(s)	297, 1222
SspI	AATATT	3 cut(s)	2749, 3412, 3999
SspMI	CTAG	6 cut(s)	1182, 1349, 1385, 1728, 1926, 3980
SstI	GAGCTC	2 cut(s)	78, 409
StuI	AGGCCT	2 cut(s)	297, 1222
StyD4I	CCNGG	8 cut(s)	42, 379, 448, 1522, 1963, 2845, 2994, 3154
StyI	CCWWGG	3 cut(s)	298, 1348, 3555
SwaI	ATTTAAAT	1 cut(s)	1371
TaiI	ACGT	2 cut(s)	3831, 3867
TatI	WGTACW	5 cut(s)	374, 665, 1243, 1781, 1910
TauI	GCSGC	2 cut(s)	369, 3978
TscAI	CASTG	7 cut(s)	994, 1660, 1983, 2653, 3850, 3926, 3974
TseFI	GTSAC	4 cut(s)	656, 2258, 2752, 3247
Tsp45I	GTSAC	4 cut(s)	656, 2258, 2752, 3247
TspDTI	ATGAA	9 cut(s)	231, 821, 1051, 1187, 1891, 2388, 2648, 3147, 3715
TspGWI	ACGGA	2 cut(s)	75, 2593
TspRI	CASTG	7 cut(s)	994, 1660, 1983, 2653, 3850, 3926, 3974
Vha464I	CTTAAG	1 cut(s)	2762
VpaK11BI	GGWCC	1 cut(s)	2227
XagI	CCTNNNNNAGG	2 cut(s)	1388, 1575
XapI	RAATTY	7 cut(s)	693, 1090, 1372, 1496, 1943, 3074, 3108
XceI	RCATGY	5 cut(s)	388, 1472, 2123, 2544, 2894
XcmI	CCANNNNNNNNNTGG	3 cut(s)	341, 1420, 2908
XhoI	CTCGAG	1 cut(s)	3114
XmaJI	CCTAGG	1 cut(s)	1348
XmiI	GTMKAC	4 cut(s)	213, 1860, 2420, 3002
XmnI	GAANNNNTTC	1 cut(s)	1288
XspI	CTAG	6 cut(s)	1182, 1349, 1385, 1728, 1926, 3980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.