AT1G08650
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004122
Physical Location & Seq
Forward (+)
2752124 .. 2753809
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G08650.1

Sequence Viewer

Length: 855 bp
ATGACTTGCAGCCAAACACTCGGAAACAACAACACCAACAAATACCAGATCTGCGAGGAGATCGGCCGTGGCCGTTTCGGCACCGTCTCTCGCGTCTACGCTCCGGCCACCGGTGATTTCTTCGCTTGCAAAACCATCGACAAAGCCTCTCTCTCCGACGACCTTGACCGTGCCTGTCTCGACAACGAGCCTAAGCTCATGGCTCTCTTGTCCTATCACCCTAACATCGTTCAAATCCATGATCTCATCGACACTGATTCGACTCTCTCCATCTTCATGGAGCTCGTCCATCCCTCTGTTTCGATCTACGACCGTCTCGTCTCTTCTGGAACCTTCTTTGAGCCTCAGACGGCGTCGTTCGCTAAACAGATTCTCCAGGCGCTTTCGCATTGTCACCGATACGGCGTCGTTCACAGGGATATTAAACCGGAGAACATTCTGGTAGATCTACGAAACGATACGGTTAAGATCTGCGATTTCGGTTCAGGGATTTGGTTAGGTGAGGGAGAGACCACCGAAGGAGTCGTCGGAACGCCGTATTACGTGGCGCCGGAGGTTTTAATGGGATATTCTTACGGAGAGAAGGTCGATCTATGGAGCGCCGGCGTCGTTTTGTACACGATGCTCGCTGGAACTCCACCGTTTTACGGCGAGACAGCTGAAGAGATATTCGAAGCGGTACTCAGAGGAAATCTCCGTTTTCCGACGAAGATATTCCGGGGGGTTTCATCTATGGCTAAAGATTTCCTAAGGAAATTGATATGCAAAGATGCTTCTAGAAGATTCTCAGCCGAACAAGCATTAAGGCACCCATGGATCCAAAGAGCAGGAGAAACAGAGGAGAGATTCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009267 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009636 GO:0009931 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010857 GO:0014070 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0035556 GO:0036211 GO:0042221 GO:0042493 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046677 GO:0046777 GO:0046898 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060992 GO:0065007 GO:0071496 GO:0071704 GO:0097305 GO:0140096 GO:1901564 GO:1901654 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04012 ko04020 ko04024 ko04066 ko04114 ko04217 ko04261 ko04310 ko04360 ko04713 ko04720 ko04722 ko04725 ko04728 ko04740 ko04745 ko04750 ko04911 ko04912 ko04916 ko04921 ko04922 ko04925 ko04934 ko04971 ko05031 ko05152 ko05200 ko05205 ko05214 map04012 map04020 map04024 map04066 map04114 map04217 map04261 map04310 map04360 map04713 map04720 map04722 map04725 map04728 map04740 map04745 map04750 map04911 map04912 map04916 map04921 map04922 map04925 map04934 map04971 map05031 map05152 map05200 map05205 map05214
Pfam Domains
Protein Families
Pkinase

Protein Analysis

284

Amino Acids

31.83

Weight (kDa)

5.55

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 273 1.8e-70 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 261 1e-27 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 317
AccB1I GGYRCC 3 cut(s) 80, 547, 807
AccI GTMKAC 1 cut(s) 96
AccII CGCG 1 cut(s) 93
AciI CCGC 1 cut(s) 677
AclWI GGATC 2 cut(s) 811, 824
AcoI YGGCCR 3 cut(s) 64, 70, 105
AcuI CTGAAG 1 cut(s) 681
AcyI GRCGYC 4 cut(s) 353, 405, 548, 606
AfaI GTAC 2 cut(s) 617, 681
AfiI CCNNNNNNNGG 2 cut(s) 110, 647
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 3 cut(s) 196, 283, 659
AluI AGCT 3 cut(s) 196, 283, 659
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 6 cut(s) 91, 182, 320, 325, 503, 647
AlwI GGATC 2 cut(s) 811, 824
AoxI GGCC 3 cut(s) 64, 70, 105
ApeKI GCWGC 1 cut(s) 9
ArsI GACNNNNNNTTYG 2 cut(s) 510, 542
AsiGI ACCGGT 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 713
AspLEI GCGC 3 cut(s) 382, 550, 602
AsuC2I CCSGG 1 cut(s) 719
AsuHPI GGTGA 4 cut(s) 125, 209, 386, 512
AsuII TTCGAA 1 cut(s) 672
AxyI CCTNAGG 1 cut(s) 749
BamHI GGATCC 1 cut(s) 816
BanI GGYRCC 3 cut(s) 80, 547, 807
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 21
BccI CCATC 3 cut(s) 143, 278, 297
BceAI ACGGC 6 cut(s) 51, 57, 366, 418, 520, 664
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 377
BcnI CCSGG 1 cut(s) 719
BcoDI GTCTC 6 cut(s) 91, 182, 320, 325, 503, 647
BfaI CTAG 2 cut(s) 777, 853
BfoI RGCGCY 3 cut(s) 383, 551, 603
BglI GCCNNNNNGGC 1 cut(s) 78
BglII AGATCT 3 cut(s) 48, 445, 468
BisI GCNGC 1 cut(s) 10
BlsI GCNGC 1 cut(s) 11
Bme1390I CCNGG 2 cut(s) 377, 719
BmiI GGNNCC 5 cut(s) 82, 331, 549, 809, 818
BmrFI CCNGG 2 cut(s) 377, 719
BmsI GCATC 2 cut(s) 612, 760
BplI GAGNNNNNCTC 2 cut(s) 678, 710
BpmI CTGGAG 1 cut(s) 359
Bpu10I CCTNAGC 1 cut(s) 192
Bpu14I TTCGAA 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 719
BsaAI YACGTR 1 cut(s) 544
BsaHI GRCGYC 4 cut(s) 353, 405, 548, 606
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 3 cut(s) 67, 718, 812
BsaWI WCCGGW 2 cut(s) 110, 427
BsaXI ACNNNNNCTCC 4 cut(s) 357, 387, 570, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 647
Bse118I RCCGGY 2 cut(s) 110, 602
Bse21I CCTNAGG 1 cut(s) 749
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 3 cut(s) 67, 718, 812
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 110, 647
BseMII CTCAG 3 cut(s) 359, 697, 801
BseRI GAGGAG 2 cut(s) 71, 854
BseX3I CGGCCG 1 cut(s) 64
BseXI GCAGC 1 cut(s) 21
Bsh1236I CGCG 1 cut(s) 93
Bsh1285I CGRYCG 2 cut(s) 67, 313
BshFI GGCC 3 cut(s) 66, 72, 107
BshNI GGYRCC 3 cut(s) 80, 547, 807
BshTI ACCGGT 1 cut(s) 110
BsiEI CGRYCG 2 cut(s) 67, 313
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 6 cut(s) 104, 111, 428, 551, 603, 718
BslI CCNNNNNNNGG 2 cut(s) 110, 647
BsmAI GTCTC 6 cut(s) 91, 182, 320, 325, 503, 647
BsmBI CGTCTC 3 cut(s) 91, 320, 325
BsnI GGCC 3 cut(s) 66, 72, 107
Bso31I GGTCTC 1 cut(s) 503
Bsp119I TTCGAA 1 cut(s) 672
Bsp1286I GDGCHC 1 cut(s) 285
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 8 cut(s) 48, 60, 241, 303, 445, 468, 589, 816
Bsp19I CCATGG 1 cut(s) 812
BspACI CCGC 1 cut(s) 677
BspANI GGCC 3 cut(s) 66, 72, 107
BspCNI CTCAG 3 cut(s) 358, 696, 800
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 5 cut(s) 82, 331, 549, 809, 818
BspPI GGATC 2 cut(s) 811, 824
BspT104I TTCGAA 1 cut(s) 672
BspT107I GGYRCC 3 cut(s) 80, 547, 807
BspTNI GGTCTC 1 cut(s) 503
BsrFI RCCGGY 2 cut(s) 110, 602
BsrGI TGTACA 1 cut(s) 615
BssAI RCCGGY 2 cut(s) 110, 602
BssECI CCNNGG 3 cut(s) 67, 718, 812
BssMI GATC 8 cut(s) 48, 60, 241, 303, 445, 468, 589, 816
BssNI GRCGYC 4 cut(s) 353, 405, 548, 606
BssT1I CCWWGG 1 cut(s) 812
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 5 cut(s) 85, 170, 314, 463, 642
Bst6I CTCTTC 2 cut(s) 328, 657
BstACI GRCGYC 4 cut(s) 353, 405, 548, 606
BstAUI TGTACA 1 cut(s) 615
BstBAI YACGTR 1 cut(s) 544
BstBI TTCGAA 1 cut(s) 672
BstC8I GCNNGC 3 cut(s) 127, 604, 627
BstDEI CTNAG 5 cut(s) 192, 345, 683, 749, 787
BstDSI CCRYGG 2 cut(s) 67, 812
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 3 cut(s) 383, 551, 603
BstHHI GCGC 3 cut(s) 382, 550, 602
BstKTI GATC 8 cut(s) 51, 63, 244, 306, 448, 471, 592, 819
BstMAI GTCTC 6 cut(s) 91, 182, 320, 325, 503, 647
BstMBI GATC 8 cut(s) 48, 60, 241, 303, 445, 468, 589, 816
BstMCI CGRYCG 2 cut(s) 67, 313
BstMWI GCNNNNNNNGC 3 cut(s) 78, 359, 797
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 375, 717
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 21
BstX2I RGATCY 4 cut(s) 48, 445, 468, 816
BstXI CCANNNNNNTGG 1 cut(s) 277
BstYI RGATCY 4 cut(s) 48, 445, 468, 816
BstZI CGGCCG 1 cut(s) 64
Bsu36I CCTNAGG 1 cut(s) 749
BsuRI GGCC 3 cut(s) 66, 72, 107
BtgI CCRYGG 2 cut(s) 67, 812
BtsCI GGATG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 252
Cac8I GCNNGC 3 cut(s) 127, 604, 627
CfoI GCGC 3 cut(s) 382, 550, 602
Cfr10I RCCGGY 2 cut(s) 110, 602
CseI GACGC 4 cut(s) 82, 342, 394, 595
Csp6I GTAC 2 cut(s) 616, 680
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 4 cut(s) 199, 239, 277, 813
CviQI GTAC 2 cut(s) 616, 680
DdeI CTNAG 5 cut(s) 192, 345, 683, 749, 787
DinI GGCGCC 1 cut(s) 549
DpnI GATC 8 cut(s) 50, 62, 243, 305, 447, 470, 591, 818
DpnII GATC 8 cut(s) 48, 60, 241, 303, 445, 468, 589, 816
DrdI GACNNNNNNGTC 1 cut(s) 317
DseDI GACNNNNNNGTC 1 cut(s) 317
EaeI YGGCCR 3 cut(s) 64, 70, 105
EagI CGGCCG 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 328, 657
EarI CTCTTC 2 cut(s) 328, 657
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 812
Eco24I GRGCYC 1 cut(s) 285
Eco31I GGTCTC 1 cut(s) 503
Eco52I CGGCCG 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 681
Eco81I CCTNAGG 1 cut(s) 749
EcoICRI GAGCTC 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 812
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 1 cut(s) 549
EheI GGCGCC 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 812
Esp3I CGTCTC 3 cut(s) 91, 320, 325
FaeI CATG 4 cut(s) 202, 242, 280, 816
FaiI YATR 7 cut(s) 200, 240, 278, 595, 734, 763, 814
FatI CATG 4 cut(s) 198, 238, 276, 812
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 10
FokI GGATG 1 cut(s) 276
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 1 cut(s) 10
FspBI CTAG 2 cut(s) 777, 853
GlaI GCGC 3 cut(s) 381, 549, 601
GluI GCNGC 1 cut(s) 10
GsuI CTGGAG 1 cut(s) 359
HaeII RGCGCY 3 cut(s) 383, 551, 603
HaeIII GGCC 3 cut(s) 66, 72, 107
HapII CCGG 6 cut(s) 104, 111, 428, 551, 603, 718
HgaI GACGC 4 cut(s) 82, 342, 394, 595
HhaI GCGC 3 cut(s) 382, 550, 602
Hin1I GRCGYC 4 cut(s) 353, 405, 548, 606
Hin1II CATG 4 cut(s) 202, 242, 280, 816
Hin6I GCGC 3 cut(s) 380, 548, 600
HinP1I GCGC 3 cut(s) 380, 548, 600
HinfI GANTC 6 cut(s) 257, 262, 370, 522, 783, 846
HpaII CCGG 6 cut(s) 104, 111, 428, 551, 603, 718
HphI GGTGA 4 cut(s) 125, 209, 386, 512
Hpy166II GTNNAC 3 cut(s) 97, 412, 618
Hpy188I TCNGA 6 cut(s) 23, 157, 348, 530, 686, 705
Hpy188III TCNNGA 3 cut(s) 179, 327, 777
Hpy8I GTNNAC 3 cut(s) 97, 412, 618
Hpy99I CGWCG 6 cut(s) 161, 358, 410, 530, 611, 709
HpyAV CCTTC 3 cut(s) 343, 512, 577
HpyCH4III ACNGT 5 cut(s) 85, 170, 314, 463, 642
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 3 cut(s) 9, 129, 765
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 359, 797
HpyF3I CTNAG 5 cut(s) 192, 345, 683, 749, 787
HpySE526I ACGT 1 cut(s) 543
Hsp92I GRCGYC 4 cut(s) 353, 405, 548, 606
Hsp92II CATG 4 cut(s) 202, 242, 280, 816
HspAI GCGC 3 cut(s) 380, 548, 600
KasI GGCGCC 1 cut(s) 547
KroI GCCGGC 1 cut(s) 602
KroNI GCCGGC 1 cut(s) 604
Kzo9I GATC 8 cut(s) 48, 60, 241, 303, 445, 468, 589, 816
LmnI GCTCC 3 cut(s) 106, 280, 597
Lsp1109I GCAGC 1 cut(s) 21
LweI GCATC 2 cut(s) 612, 760
MaeI CTAG 2 cut(s) 777, 853
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 1 cut(s) 392
MalI GATC 8 cut(s) 50, 62, 243, 305, 447, 470, 591, 818
MboI GATC 8 cut(s) 48, 60, 241, 303, 445, 468, 589, 816
MboII GAAGA 6 cut(s) 112, 265, 315, 674, 721, 792
MflI RGATCY 4 cut(s) 48, 445, 468, 816
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 1 cut(s) 755
Mly113I GGCGCC 1 cut(s) 548
MlyI GAGTC 2 cut(s) 256, 531
MmeI TCCRAC 3 cut(s) 180, 508, 728
MnlI CCTC 8 cut(s) 49, 157, 304, 354, 496, 547, 680, 832
MreI CGCCGGCG 1 cut(s) 602
MroNI GCCGGC 1 cut(s) 602
MroXI GAANNNNTTC 1 cut(s) 713
MseI TTAA 4 cut(s) 423, 465, 560, 803
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 6 cut(s) 104, 111, 428, 551, 603, 718
MspR9I CCNGG 2 cut(s) 377, 719
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 78, 359, 797
NaeI GCCGGC 1 cut(s) 604
NarI GGCGCC 1 cut(s) 548
NciI CCSGG 1 cut(s) 719
NcoI CCATGG 1 cut(s) 812
NdeII GATC 8 cut(s) 48, 60, 241, 303, 445, 468, 589, 816
NgoMIV GCCGGC 1 cut(s) 602
NlaIII CATG 4 cut(s) 202, 242, 280, 816
NlaIV GGNNCC 5 cut(s) 82, 331, 549, 809, 818
NmuCI GTSAC 1 cut(s) 392
NspV TTCGAA 1 cut(s) 672
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PdiI GCCGGC 1 cut(s) 604
PdmI GAANNNNTTC 1 cut(s) 713
PfeI GAWTC 4 cut(s) 257, 370, 783, 846
PflFI GACNNNGTC 1 cut(s) 352
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 1 cut(s) 11
PleI GAGTC 2 cut(s) 256, 530
PluTI GGCGCC 1 cut(s) 551
PpsI GAGTC 2 cut(s) 256, 530
Ppu21I YACGTR 1 cut(s) 544
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 5 cut(s) 82, 331, 549, 809, 818
PsuI RGATCY 4 cut(s) 48, 445, 468, 816
PsyI GACNNNGTC 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 2 cut(s) 617, 681
RsaNI GTAC 2 cut(s) 616, 680
RseI CAYNNNNRTG 1 cut(s) 275
SacI GAGCTC 1 cut(s) 285
SaqAI TTAA 4 cut(s) 423, 465, 560, 803
SatI GCNGC 1 cut(s) 10
Sau3AI GATC 8 cut(s) 48, 60, 241, 303, 445, 468, 589, 816
SchI GAGTC 2 cut(s) 256, 531
ScrFI CCNGG 2 cut(s) 377, 719
SduI GDGCHC 1 cut(s) 285
SetI ASST 9 cut(s) 165, 198, 285, 335, 502, 546, 558, 588, 661
SfaNI GCATC 2 cut(s) 612, 760
SfoI GGCGCC 1 cut(s) 549
SfuI TTCGAA 1 cut(s) 672
SgrAI CRCCGGYG 2 cut(s) 110, 602
SmiMI CAYNNNNRTG 1 cut(s) 275
Sse9I AATT 1 cut(s) 755
SsiI CCGC 1 cut(s) 677
SspDI GGCGCC 1 cut(s) 547
SspMI CTAG 2 cut(s) 777, 853
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 375, 717
StyI CCWWGG 1 cut(s) 812
TaaI ACNGT 5 cut(s) 85, 170, 314, 463, 642
TaiI ACGT 1 cut(s) 546
TaqI TCGA 7 cut(s) 138, 180, 249, 260, 302, 588, 672
TasI AATT 1 cut(s) 755
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 4 cut(s) 257, 370, 783, 846
Tru1I TTAA 4 cut(s) 423, 465, 560, 803
Tru9I TTAA 4 cut(s) 423, 465, 560, 803
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 1 cut(s) 9
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 3 cut(s) 265, 717, 838
TspGWI ACGGA 2 cut(s) 591, 686
TspRI CASTG 1 cut(s) 259
Tth111I GACNNNGTC 1 cut(s) 352
XbaI TCTAGA 1 cut(s) 776
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 713
XspI CTAG 2 cut(s) 777, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.