Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004122

Physical Location & Seq

Genomic Coordinates Reverse (-)

21495466 .. 21496869

Gene Structure 1404 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1217500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 828 bp

ATGACCGAGGCACTGAAGCGGGACTACCAGCTGTGCGAGGAGCTCGGCCGCGGACGGTTCGGCGTCGTTTTCAAAGCGATCAGAAACGGCGACGCTTTCGCCGTCAAATCTATCGACAAGCGAATCGCCGACTCGGGCGACTCGCTCGACGCCCAATGCCTCTTGACGGAGCCCAAGATTCTGAAGCTCCTGGGTTCGCACCCGAACGTTGTCAACCTCCACGACCTCTACGAGGACGACGACCACCTCCACATGGTCCTCGACCTCTGCGACTCGCCCGATCTCTACCGTCGAGTCACTCTCCGGGTCTTCTCCGAGTCCGAGGCCTCCTCGGTCATGACTCAGCTCTTGCACGCTGTCGCGCACTGCCACAGCCTCGGCGTCGCTCACCGTGACATCAAGCCGGACAACATCCTCTTCGACGGCCAGGAACGGCTCCGGCTCGCCGATTTCGGATCGGCCGAGAATTTCGGCGAGGGGGAGAAGATGGGCGGTGTTGTCGGAACGCCGTACTACGTGGCGCCGGAGGTGGTGGCGGGAAGGGAGTACGGGGAGAAAGTCGACGTCTGGAGCTGCGGCGTCGTTTTGTACATAATGCTGGCCGGGTTCCCGCCGTTTTACGGCGAAACAGCGGCGGAGATCTTCGATGCGGTGCTAAGAGCGAATTTGAGATTTCCGGCAAGGGTTTTTAACTCCTTGTCGGCCGCCCTTAAGGATTTGTTGCGGTGCATGCTGTGTAAGGACGTTTCTAGAAGGTTCTCTGCTGAACAAGTCCTCAGGCATCCATGGATTACAAGTGGGGGAGGAGGGGGCCAGAGCTGTGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009636 GO:0009931 GO:0009987 GO:0010033 GO:0010243 GO:0010857 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046677 GO:0046777 GO:0046898 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060992 GO:0065007 GO:0071704 GO:0097305 GO:0140096 GO:1901564 GO:1901654 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04012 ko04020 ko04024 ko04066 ko04114 ko04217 ko04261 ko04310 ko04360 ko04713 ko04720 ko04722 ko04725 ko04728 ko04740 ko04745 ko04750 ko04911 ko04912 ko04916 ko04921 ko04922 ko04925 ko04934 ko04971 ko05031 ko05152 ko05200 ko05205 ko05214 map04012 map04020 map04024 map04066 map04114 map04217 map04261 map04310 map04360 map04713 map04720 map04722 map04725 map04728 map04740 map04745 map04750 map04911 map04912 map04916 map04921 map04922 map04925 map04934 map04971 map05031 map05152 map05200 map05205 map05214

Pfam Domains

Protein Families

Pkinase

Protein Analysis

276

Amino Acids

30.34

Weight (kDa)

5.6

Isoelectric Point (pI)

44.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	9 - 264	9.4e-74	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	10 - 259	3e-29	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08650 AT1G08650 AT3G04530
fragaria_vesca	FvH4_4g03560 FvH4_6g02090 FvH4_6g08930
malus_domestica	MD04G1229200.v1.1 MD16G1217500.v1.1
prunus_persica	Prupe.1G038800_v2.0.a1 Prupe.6G349100_v2.0.a1
pyrus_communis	pycom12g22590
rosa_chinensis	RchiOBHm_Chr3g0449921 RchiOBHm_Chr4g0392911
rosa_laevigata	RLG00000009782 RLG00000025775
rosa_multiflora	Rmu_co8401231.1_g000001 Rmu_sc0002646.1_g000001 Rmu_sc0005524.1_g000005 Rmu_sc0008339.1_g000031
rosa_roxburghii	Rroxscaffold_5G00338030 Rroxscaffold_6G00427720
rosa_rugosa	Rorug02G0624000 Rorug03G0341100
rosa_samantha	Rh3AG023100 Rh3BG023700 Rh3CG022800 Rh3DG023600 Rh4AG044800 Rh4BG040600 Rh4CG048200 Rh4DG042400
rosa_wichuraiana	Rw3G001850 Rw4G003510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	567
AccB1I	GGYRCC	1 cut(s)	520
AccI	GTMKAC	1 cut(s)	561
AccII	CGCG	2 cut(s)	51, 362
AclI	AACGTT	1 cut(s)	207
AclWI	GGATC	1 cut(s)	463
AcoI	YGGCCR	5 cut(s)	46, 424, 459, 600, 702
AcsI	RAATTY	2 cut(s)	466, 664
AcuI	CTGAAG	2 cut(s)	35, 203
AcyI	GRCGYC	6 cut(s)	63, 150, 381, 521, 564, 579
AfaI	GTAC	3 cut(s)	512, 548, 590
AfiI	CCNNNNNNNGG	4 cut(s)	166, 232, 253, 620
AflII	CTTAAG	1 cut(s)	710
AgsI	TTSAA	1 cut(s)	73
AjnI	CCWGG	2 cut(s)	189, 426
AluBI	AGCT	6 cut(s)	31, 43, 187, 346, 573, 819
AluI	AGCT	6 cut(s)	31, 43, 187, 346, 573, 819
Alw21I	GWGCWC	1 cut(s)	45
AlwI	GGATC	1 cut(s)	463
Ama87I	CYCGRG	1 cut(s)	133
AoxI	GGCC	7 cut(s)	46, 324, 424, 459, 600, 702, 811
ApeKI	GCWGC	1 cut(s)	573
ApoI	RAATTY	2 cut(s)	466, 664
AspLEI	GCGC	2 cut(s)	364, 523
AspS9I	GGNCC	2 cut(s)	256, 811
AsuC2I	CCSGG	2 cut(s)	305, 604
AsuHPI	GGTGA	1 cut(s)	380
AvaI	CYCGRG	1 cut(s)	133
AvaII	GGWCC	1 cut(s)	256
AxyI	CCTNAGG	1 cut(s)	776
BanI	GGYRCC	1 cut(s)	520
BanII	GRGCYC	2 cut(s)	45, 174
BarI	GAAGNNNNNNTAC	2 cut(s)	8, 40
BbsI	GAAGAC	1 cut(s)	301
Bbv12I	GWGCWC	1 cut(s)	45
BbvI	GCAGC	1 cut(s)	560
BccI	CCATC	1 cut(s)	481
BceAI	ACGGC	7 cut(s)	86, 103, 439, 449, 493, 598, 637
BciT130I	CCWGG	2 cut(s)	191, 428
BcnI	CCSGG	2 cut(s)	305, 604
BfaI	CTAG	1 cut(s)	750
BfoI	RGCGCY	1 cut(s)	524
BfrI	CTTAAG	1 cut(s)	710
BglII	AGATCT	1 cut(s)	639
BisI	GCNGC	5 cut(s)	49, 574, 577, 633, 705
BlsI	GCNGC	5 cut(s)	50, 575, 578, 634, 706
Bme1390I	CCNGG	4 cut(s)	191, 305, 428, 604
Bme18I	GGWCC	1 cut(s)	256
BmeT110I	CYCGRG	1 cut(s)	133
BmgT120I	GGNCC	2 cut(s)	256, 811
BmiI	GGNNCC	5 cut(s)	171, 437, 522, 608, 812
BmrFI	CCNGG	4 cut(s)	191, 305, 428, 604
BmsI	GCATC	2 cut(s)	637, 790
BpiI	GAAGAC	1 cut(s)	301
BplI	GAGNNNNNCTC	4 cut(s)	285, 317, 314, 346
BpmI	CTGGAG	1 cut(s)	589
BpuMI	CCSGG	2 cut(s)	305, 604
BsaAI	YACGTR	1 cut(s)	517
BsaHI	GRCGYC	6 cut(s)	63, 150, 381, 521, 564, 579
BsaJI	CCNNGG	7 cut(s)	6, 49, 190, 321, 330, 376, 785
Bsc4I	CCNNNNNNNGG	4 cut(s)	166, 232, 253, 620
Bse21I	CCTNAGG	1 cut(s)	776
BseBI	CCWGG	2 cut(s)	191, 428
BseDI	CCNNGG	7 cut(s)	6, 49, 190, 321, 330, 376, 785
BseGI	GGATG	2 cut(s)	411, 781
BseLI	CCNNNNNNNGG	4 cut(s)	166, 232, 253, 620
BseMII	CTCAG	2 cut(s)	356, 790
BseRI	GAGGAG	3 cut(s)	53, 319, 819
BseX3I	CGGCCG	3 cut(s)	46, 459, 702
BseXI	GCAGC	1 cut(s)	560
Bsh1236I	CGCG	2 cut(s)	51, 362
Bsh1285I	CGRYCG	3 cut(s)	49, 462, 705
BshFI	GGCC	7 cut(s)	48, 326, 426, 461, 602, 704, 813
BshNI	GGYRCC	1 cut(s)	520
BsiEI	CGRYCG	3 cut(s)	49, 462, 705
BsiHKAI	GWGCWC	1 cut(s)	45
BsiHKCI	CYCGRG	1 cut(s)	133
BsiSI	CCGG	6 cut(s)	304, 404, 439, 524, 603, 677
BslFI	GGGAC	1 cut(s)	35
BslI	CCNNNNNNNGG	4 cut(s)	166, 232, 253, 620
BsmFI	GGGAC	1 cut(s)	35
BsnI	GGCC	7 cut(s)	48, 326, 426, 461, 602, 704, 813
BsoBI	CYCGRG	1 cut(s)	133
Bsp1286I	GDGCHC	2 cut(s)	45, 174
Bsp1407I	TGTACA	1 cut(s)	588
Bsp143I	GATC	4 cut(s)	78, 280, 455, 639
Bsp19I	CCATGG	1 cut(s)	785
BspANI	GGCC	7 cut(s)	48, 326, 426, 461, 602, 704, 813
BspCNI	CTCAG	2 cut(s)	355, 789
BspFNI	CGCG	2 cut(s)	51, 362
BspHI	TCATGA	1 cut(s)	336
BspLI	GGNNCC	5 cut(s)	171, 437, 522, 608, 812
BspPI	GGATC	1 cut(s)	463
BspT107I	GGYRCC	1 cut(s)	520
BspTI	CTTAAG	1 cut(s)	710
BsrGI	TGTACA	1 cut(s)	588
BssECI	CCNNGG	7 cut(s)	6, 49, 190, 321, 330, 376, 785
BssMI	GATC	4 cut(s)	78, 280, 455, 639
BssNI	GRCGYC	6 cut(s)	63, 150, 381, 521, 564, 579
BssT1I	CCWWGG	1 cut(s)	785
Bst2UI	CCWGG	2 cut(s)	191, 428
Bst4CI	ACNGT	3 cut(s)	57, 290, 392
Bst6I	CTCTTC	1 cut(s)	422
BstACI	GRCGYC	6 cut(s)	63, 150, 381, 521, 564, 579
BstAFI	CTTAAG	1 cut(s)	710
BstAUI	TGTACA	1 cut(s)	588
BstBAI	YACGTR	1 cut(s)	517
BstC8I	GCNNGC	4 cut(s)	354, 444, 600, 731
BstDEI	CTNAG	3 cut(s)	342, 656, 776
BstDSI	CCRYGG	2 cut(s)	49, 785
BstENI	CCTNNNNNAGG	1 cut(s)	230
BstF5I	GGATG	2 cut(s)	411, 781
BstFNI	CGCG	2 cut(s)	51, 362
BstH2I	RGCGCY	1 cut(s)	524
BstHHI	GCGC	2 cut(s)	364, 523
BstKTI	GATC	4 cut(s)	81, 283, 458, 642
BstMBI	GATC	4 cut(s)	78, 280, 455, 639
BstMCI	CGRYCG	3 cut(s)	49, 462, 705
BstMWI	GCNNNNNNNGC	1 cut(s)	730
BstNI	CCWGG	2 cut(s)	191, 428
BstNSI	RCATGY	1 cut(s)	733
BstSCI	CCNGG	4 cut(s)	189, 303, 426, 602
BstUI	CGCG	2 cut(s)	51, 362
BstV1I	GCAGC	1 cut(s)	560
BstV2I	GAAGAC	1 cut(s)	301
BstX2I	RGATCY	1 cut(s)	639
BstXI	CCANNNNNNTGG	1 cut(s)	821
BstYI	RGATCY	1 cut(s)	639
BstZI	CGGCCG	3 cut(s)	46, 459, 702
Bsu36I	CCTNAGG	1 cut(s)	776
BsuRI	GGCC	7 cut(s)	48, 326, 426, 461, 602, 704, 813
BtgI	CCRYGG	2 cut(s)	49, 785
BtsCI	GGATG	2 cut(s)	411, 781
BtsI	GCAGTG	1 cut(s)	364
BtsIMutI	CAGTG	2 cut(s)	11, 364
Cac8I	GCNNGC	4 cut(s)	354, 444, 600, 731
CciI	TCATGA	1 cut(s)	336
CfoI	GCGC	2 cut(s)	364, 523
Cfr13I	GGNCC	2 cut(s)	256, 811
Cfr42I	CCGCGG	1 cut(s)	52
CseI	GACGC	5 cut(s)	52, 101, 158, 370, 568
Csp6I	GTAC	3 cut(s)	511, 547, 589
CviAII	CATG	4 cut(s)	253, 337, 730, 786
CviQI	GTAC	3 cut(s)	511, 547, 589
DdeI	CTNAG	3 cut(s)	342, 656, 776
DinI	GGCGCC	1 cut(s)	522
DpnI	GATC	4 cut(s)	80, 282, 457, 641
DpnII	GATC	4 cut(s)	78, 280, 455, 639
EaeI	YGGCCR	5 cut(s)	46, 424, 459, 600, 702
EagI	CGGCCG	3 cut(s)	46, 459, 702
Eam1104I	CTCTTC	1 cut(s)	422
EarI	CTCTTC	1 cut(s)	422
EciI	GGCGGA	1 cut(s)	650
Ecl136II	GAGCTC	1 cut(s)	43
EclXI	CGGCCG	3 cut(s)	46, 459, 702
Eco130I	CCWWGG	1 cut(s)	785
Eco147I	AGGCCT	1 cut(s)	326
Eco24I	GRGCYC	2 cut(s)	45, 174
Eco47I	GGWCC	1 cut(s)	256
Eco52I	CGGCCG	3 cut(s)	46, 459, 702
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	2 cut(s)	35, 203
Eco81I	CCTNAGG	1 cut(s)	776
Eco88I	CYCGRG	1 cut(s)	133
EcoICRI	GAGCTC	1 cut(s)	43
EcoNI	CCTNNNNNAGG	1 cut(s)	230
EcoRII	CCWGG	2 cut(s)	189, 426
EcoT14I	CCWWGG	1 cut(s)	785
EcoT38I	GRGCYC	2 cut(s)	45, 174
EgeI	GGCGCC	1 cut(s)	522
EheI	GGCGCC	1 cut(s)	522
ErhI	CCWWGG	1 cut(s)	785
FaeI	CATG	4 cut(s)	256, 340, 733, 789
FaiI	YATR	5 cut(s)	254, 338, 593, 731, 787
FaqI	GGGAC	1 cut(s)	35
FatI	CATG	4 cut(s)	252, 336, 729, 785
FauI	CCCGC	3 cut(s)	12, 529, 618
FblI	GTMKAC	1 cut(s)	561
Fnu4HI	GCNGC	5 cut(s)	49, 574, 577, 633, 705
FokI	GGATG	2 cut(s)	398, 768
FriOI	GRGCYC	2 cut(s)	45, 174
Fsp4HI	GCNGC	5 cut(s)	49, 574, 577, 633, 705
FspBI	CTAG	1 cut(s)	750
GlaI	GCGC	2 cut(s)	363, 522
GluI	GCNGC	5 cut(s)	49, 574, 577, 633, 705
GsuI	CTGGAG	1 cut(s)	589
HaeII	RGCGCY	1 cut(s)	524
HaeIII	GGCC	7 cut(s)	48, 326, 426, 461, 602, 704, 813
HapII	CCGG	6 cut(s)	304, 404, 439, 524, 603, 677
HgaI	GACGC	5 cut(s)	52, 101, 158, 370, 568
HhaI	GCGC	2 cut(s)	364, 523
Hin1I	GRCGYC	6 cut(s)	63, 150, 381, 521, 564, 579
Hin1II	CATG	4 cut(s)	256, 340, 733, 789
Hin6I	GCGC	2 cut(s)	362, 521
HinP1I	GCGC	2 cut(s)	362, 521
HincII	GTYRAC	2 cut(s)	214, 562
HindII	GTYRAC	2 cut(s)	214, 562
HinfI	GANTC	8 cut(s)	123, 131, 140, 178, 272, 294, 317, 340
HpaII	CCGG	6 cut(s)	304, 404, 439, 524, 603, 677
HphI	GGTGA	1 cut(s)	380
Hpy166II	GTNNAC	2 cut(s)	214, 562
Hpy188I	TCNGA	6 cut(s)	83, 183, 316, 322, 455, 503
Hpy188III	TCNNGA	4 cut(s)	163, 337, 568, 750
Hpy8I	GTNNAC	2 cut(s)	214, 562
Hpy99I	CGWCG	9 cut(s)	68, 95, 152, 242, 294, 386, 425, 566, 584
HpyAV	CCTTC	2 cut(s)	534, 747
HpyCH4III	ACNGT	3 cut(s)	57, 290, 392
HpyCH4IV	ACGT	4 cut(s)	207, 516, 564, 744
HpyCH4V	TGCA	2 cut(s)	352, 729
HpyF10VI	GCNNNNNNNGC	1 cut(s)	730
HpyF3I	CTNAG	3 cut(s)	342, 656, 776
HpySE526I	ACGT	4 cut(s)	207, 516, 564, 744
Hsp92I	GRCGYC	6 cut(s)	63, 150, 381, 521, 564, 579
Hsp92II	CATG	4 cut(s)	256, 340, 733, 789
HspAI	GCGC	2 cut(s)	362, 521
KasI	GGCGCC	1 cut(s)	520
KspI	CCGCGG	1 cut(s)	52
Kzo9I	GATC	4 cut(s)	78, 280, 455, 639
LmnI	GCTCC	5 cut(s)	40, 169, 192, 441, 570
Lsp1109I	GCAGC	1 cut(s)	560
LweI	GCATC	2 cut(s)	637, 790
MaeI	CTAG	1 cut(s)	750
MaeII	ACGT	4 cut(s)	207, 516, 564, 744
MaeIII	GTNAC	2 cut(s)	295, 392
MalI	GATC	4 cut(s)	80, 282, 457, 641
MboI	GATC	4 cut(s)	78, 280, 455, 639
MboII	GAAGA	4 cut(s)	301, 409, 496, 634
MflI	RGATCY	1 cut(s)	639
MhlI	GDGCHC	2 cut(s)	45, 174
MluCI	AATT	2 cut(s)	466, 664
Mly113I	GGCGCC	1 cut(s)	521
MlyI	GAGTC	6 cut(s)	125, 134, 266, 303, 326, 334
MmeI	TCCRAC	1 cut(s)	481
MseI	TTAA	2 cut(s)	690, 711
MspA1I	CMGCKG	3 cut(s)	31, 51, 632
MspCI	CTTAAG	1 cut(s)	710
MspI	CCGG	6 cut(s)	304, 404, 439, 524, 603, 677
MspR9I	CCNGG	4 cut(s)	191, 305, 428, 604
MvaI	CCWGG	2 cut(s)	191, 428
MvnI	CGCG	2 cut(s)	51, 362
MwoI	GCNNNNNNNGC	1 cut(s)	730
NarI	GGCGCC	1 cut(s)	521
NciI	CCSGG	2 cut(s)	305, 604
NcoI	CCATGG	1 cut(s)	785
NdeII	GATC	4 cut(s)	78, 280, 455, 639
NlaIII	CATG	4 cut(s)	256, 340, 733, 789
NlaIV	GGNNCC	5 cut(s)	171, 437, 522, 608, 812
NmeAIII	GCCGAG	3 cut(s)	24, 357, 487
NmuCI	GTSAC	2 cut(s)	295, 392
NspI	RCATGY	1 cut(s)	733
PaeI	GCATGC	1 cut(s)	733
PagI	TCATGA	1 cut(s)	336
PceI	AGGCCT	1 cut(s)	326
PcsI	WCGNNNNNNNCGW	5 cut(s)	99, 228, 237, 267, 459
PfeI	GAWTC	2 cut(s)	123, 178
PkrI	GCNGC	5 cut(s)	50, 575, 578, 634, 706
PleI	GAGTC	6 cut(s)	125, 134, 266, 302, 325, 334
PluTI	GGCGCC	1 cut(s)	524
PpsI	GAGTC	6 cut(s)	125, 134, 266, 302, 325, 334
Ppu21I	YACGTR	1 cut(s)	517
Psp124BI	GAGCTC	1 cut(s)	45
Psp1406I	AACGTT	1 cut(s)	207
Psp6I	CCWGG	2 cut(s)	189, 426
PspGI	CCWGG	2 cut(s)	189, 426
PspN4I	GGNNCC	5 cut(s)	171, 437, 522, 608, 812
PspPI	GGNCC	2 cut(s)	256, 811
PsuI	RGATCY	1 cut(s)	639
PvuII	CAGCTG	1 cut(s)	31
RsaI	GTAC	3 cut(s)	512, 548, 590
RsaNI	GTAC	3 cut(s)	511, 547, 589
SacI	GAGCTC	1 cut(s)	45
SacII	CCGCGG	1 cut(s)	52
SalI	GTCGAC	1 cut(s)	560
SaqAI	TTAA	2 cut(s)	690, 711
SatI	GCNGC	5 cut(s)	49, 574, 577, 633, 705
Sau3AI	GATC	4 cut(s)	78, 280, 455, 639
Sau96I	GGNCC	2 cut(s)	256, 811
SchI	GAGTC	6 cut(s)	125, 134, 266, 303, 326, 334
ScrFI	CCNGG	4 cut(s)	191, 305, 428, 604
SduI	GDGCHC	2 cut(s)	45, 174
SfaNI	GCATC	2 cut(s)	637, 790
SfoI	GGCGCC	1 cut(s)	522
Sfr303I	CCGCGG	1 cut(s)	52
SgrBI	CCGCGG	1 cut(s)	52
SinI	GGWCC	1 cut(s)	256
SmlI	CTYRAG	1 cut(s)	710
SmoI	CTYRAG	1 cut(s)	710
SphI	GCATGC	1 cut(s)	733
Sse9I	AATT	2 cut(s)	466, 664
SseBI	AGGCCT	1 cut(s)	326
SspDI	GGCGCC	1 cut(s)	520
SspMI	CTAG	1 cut(s)	750
SstI	GAGCTC	1 cut(s)	45
StuI	AGGCCT	1 cut(s)	326
StyD4I	CCNGG	4 cut(s)	189, 303, 426, 602
StyI	CCWWGG	1 cut(s)	785
TaaI	ACNGT	3 cut(s)	57, 290, 392
TaiI	ACGT	4 cut(s)	210, 519, 567, 747
TaqI	TCGA	7 cut(s)	114, 147, 261, 292, 420, 561, 645
TaqII	GACCGA	2 cut(s)	20, 322
TasI	AATT	2 cut(s)	466, 664
TatI	WGTACW	1 cut(s)	588
TauI	GCSGC	4 cut(s)	51, 579, 635, 707
TfiI	GAWTC	2 cut(s)	123, 178
Tru1I	TTAA	2 cut(s)	690, 711
Tru9I	TTAA	2 cut(s)	690, 711
TscAI	CASTG	2 cut(s)	18, 371
TseFI	GTSAC	2 cut(s)	295, 392
TseI	GCWGC	1 cut(s)	573
Tsp45I	GTSAC	2 cut(s)	295, 392
TspGWI	ACGGA	1 cut(s)	182
TspRI	CASTG	2 cut(s)	18, 371
Vha464I	CTTAAG	1 cut(s)	710
VpaK11BI	GGWCC	1 cut(s)	256
XagI	CCTNNNNNAGG	1 cut(s)	230
XapI	RAATTY	2 cut(s)	466, 664
XbaI	TCTAGA	1 cut(s)	749
XceI	RCATGY	1 cut(s)	733
XmiI	GTMKAC	1 cut(s)	561
XspI	CTAG	1 cut(s)	750
ZraI	GACGTC	1 cut(s)	565

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.