AT1G09360

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009487
Physical Location & Seq
Forward (+)
3021802 .. 3022368
567 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09360.1

Sequence Viewer

Length: 567 bp
ATGATCGTTCTTGTTCTTTATATTTTTACTCCTCACATTGAAGGAGTCGAAGCCGATGGACCAACAGAAGCACTGATCAATAGTATTTGCATAGAAAACGAAGATTATGGATTTTGCAATAAAATCATCCATGAAAAATTGAAGACACCAACGGCTACTATCAAAGAACTCACGAGTCTCATAATATACACCACTATGGCTCACGCCAGTGATACTTACATTTTCATCGATAACATCCTCCGCAAATGGCCTGGCCCTAAGGAGACGAGTGGTCTCAAGACTTGTCATGCAGTTTATAAGAGAGAGACCAATAGTTTCTTAGAAATTCGCTTTCTATTTTCAAAAGGAGAGTACGAACTTATGGATGAAGCCATTTTGTCCACAGCCAAGATCTTAGAAGAGTGTAGGGGCGATTTTCTTATTCCTCCTTACAAGGAGCCTCTGCTTGAGAAGAAGAGAGTTATGAGAATATTGATCACCATGTCTGCTGTTTCTGGACATATGGTCAAAAATGAGAAGGCTTCCTTGATTAGCTCAGTAGTTACTGCCCAGTTTTTCAATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

188

Amino Acids

21.35

Weight (kDa)

5.98

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 25 - 105 1.1e-06 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
AciI CCGC 1 cut(s) 241
AcsI RAATTY 1 cut(s) 324
AfaI GTAC 1 cut(s) 353
AgsI TTSAA 4 cut(s) 41, 142, 342, 559
AhdI GACNNNNNGTC 2 cut(s) 270, 503
AjnI CCWGG 1 cut(s) 250
AleI CACNNNNGTG 1 cut(s) 207
AluBI AGCT 1 cut(s) 534
AluI AGCT 1 cut(s) 534
Alw26I GTCTC 4 cut(s) 182, 257, 278, 299
AoxI GGCC 2 cut(s) 248, 253
ApoI RAATTY 1 cut(s) 324
AspS9I GGNCC 2 cut(s) 59, 254
AsuHPI GGTGA 1 cut(s) 469
AvaII GGWCC 1 cut(s) 59
AxyI CCTNAGG 1 cut(s) 258
BauI CACGAG 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 149
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 168
BciT130I CCWGG 1 cut(s) 252
BclI TGATCA 2 cut(s) 75, 474
BcoDI GTCTC 4 cut(s) 182, 257, 278, 299
BglII AGATCT 1 cut(s) 390
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 59
BmeRI GACNNNNNGTC 2 cut(s) 270, 503
BmgT120I GGNCC 2 cut(s) 59, 254
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 1 cut(s) 252
BmrI ACTGGG 1 cut(s) 544
BmuI ACTGGG 1 cut(s) 544
BpiI GAAGAC 1 cut(s) 149
BpuEI CTTGAG 2 cut(s) 260, 467
Bsa29I ATCGAT 1 cut(s) 228
BsaI GGTCTC 2 cut(s) 278, 299
Bse1I ACTGG 2 cut(s) 207, 550
Bse21I CCTNAGG 1 cut(s) 258
BseBI CCWGG 1 cut(s) 252
BseCI ATCGAT 1 cut(s) 228
BseGI GGATG 3 cut(s) 126, 234, 370
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 2 cut(s) 207, 550
BseRI GAGGAG 1 cut(s) 21
BshFI GGCC 2 cut(s) 250, 255
BshVI ATCGAT 1 cut(s) 228
BsmAI GTCTC 4 cut(s) 182, 257, 278, 299
BsmBI CGTCTC 1 cut(s) 257
BsnI GGCC 2 cut(s) 250, 255
Bso31I GGTCTC 2 cut(s) 278, 299
Bsp143I GATC 4 cut(s) 3, 75, 390, 474
BspACI CCGC 1 cut(s) 241
BspANI GGCC 2 cut(s) 250, 255
BspCNI CTCAG 1 cut(s) 548
BspDI ATCGAT 1 cut(s) 228
BspLI GGNNCC 1 cut(s) 438
BspTNI GGTCTC 2 cut(s) 278, 299
BsrI ACTGG 2 cut(s) 207, 550
BssMI GATC 4 cut(s) 3, 75, 390, 474
BssSI CACGAG 1 cut(s) 172
Bst2BI CACGAG 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 252
Bst6I CTCTTC 2 cut(s) 393, 449
BstDEI CTNAG 4 cut(s) 258, 319, 394, 535
BstF5I GGATG 3 cut(s) 126, 234, 370
BstKTI GATC 4 cut(s) 6, 78, 393, 477
BstMAI GTCTC 4 cut(s) 182, 257, 278, 299
BstMBI GATC 4 cut(s) 3, 75, 390, 474
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
Bsu15I ATCGAT 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 258
BsuRI GGCC 2 cut(s) 250, 255
BsuTUI ATCGAT 1 cut(s) 228
BtsCI GGATG 3 cut(s) 126, 234, 370
BtsIMutI CAGTG 2 cut(s) 71, 214
Cfr13I GGNCC 2 cut(s) 59, 254
ClaI ATCGAT 1 cut(s) 228
Csp6I GTAC 1 cut(s) 352
CviAII CATG 3 cut(s) 131, 287, 481
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 4 cut(s) 258, 319, 394, 535
DpnI GATC 4 cut(s) 5, 77, 392, 476
DpnII GATC 4 cut(s) 3, 75, 390, 474
DriI GACNNNNNGTC 2 cut(s) 270, 503
Eam1104I CTCTTC 2 cut(s) 393, 449
Eam1105I GACNNNNNGTC 2 cut(s) 270, 503
EarI CTCTTC 2 cut(s) 393, 449
Eco31I GGTCTC 2 cut(s) 278, 299
Eco47I GGWCC 1 cut(s) 59
Eco81I CCTNAGG 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 250
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 3 cut(s) 134, 290, 484
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FatI CATG 3 cut(s) 130, 286, 480
FauNDI CATATG 1 cut(s) 501
FbaI TGATCA 2 cut(s) 75, 474
FokI GGATG 3 cut(s) 113, 221, 377
HaeIII GGCC 2 cut(s) 250, 255
Hin1II CATG 3 cut(s) 134, 290, 484
HinfI GANTC 2 cut(s) 45, 175
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 1 cut(s) 381
Hpy188III TCNNGA 3 cut(s) 172, 277, 495
Hpy8I GTNNAC 1 cut(s) 381
HpyAV CCTTC 2 cut(s) 35, 511
HpyCH4V TGCA 3 cut(s) 90, 117, 290
HpyF3I CTNAG 4 cut(s) 258, 319, 394, 535
Hsp92II CATG 3 cut(s) 134, 290, 484
Ksp22I TGATCA 2 cut(s) 75, 474
Kzo9I GATC 4 cut(s) 3, 75, 390, 474
LmnI GCTCC 1 cut(s) 436
LpnPI CCDG 5 cut(s) 220, 237, 264, 480, 563
MaeIII GTNAC 1 cut(s) 541
MalI GATC 4 cut(s) 5, 77, 392, 476
MboI GATC 4 cut(s) 3, 75, 390, 474
MboII GAAGA 5 cut(s) 113, 154, 410, 463, 466
MflI RGATCY 1 cut(s) 390
MluCI AATT 2 cut(s) 137, 324
MlyI GAGTC 2 cut(s) 54, 184
MnlI CCTC 4 cut(s) 42, 248, 435, 450
MslI CAYNNNNRTG 2 cut(s) 194, 207
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
NdeI CATATG 1 cut(s) 501
NdeII GATC 4 cut(s) 3, 75, 390, 474
NlaIII CATG 3 cut(s) 134, 290, 484
NlaIV GGNNCC 1 cut(s) 438
OliI CACNNNNGTG 1 cut(s) 207
PleI GAGTC 2 cut(s) 53, 183
PpsI GAGTC 2 cut(s) 53, 183
PsiI TTATAA 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 438
PspPI GGNCC 2 cut(s) 59, 254
PsuI RGATCY 1 cut(s) 390
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 2 cut(s) 194, 207
Sau3AI GATC 4 cut(s) 3, 75, 390, 474
Sau96I GGNCC 2 cut(s) 59, 254
SchI GAGTC 2 cut(s) 54, 184
ScrFI CCNGG 1 cut(s) 252
SetI ASST 1 cut(s) 536
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 194, 207
SmlI CTYRAG 2 cut(s) 275, 446
SmoI CTYRAG 2 cut(s) 275, 446
Sse9I AATT 2 cut(s) 137, 324
SsiI CCGC 1 cut(s) 241
SspI AATATT 2 cut(s) 471, 562
StyD4I CCNGG 1 cut(s) 250
TaqI TCGA 2 cut(s) 48, 228
TasI AATT 2 cut(s) 137, 324
TscAI CASTG 2 cut(s) 78, 214
TspDTI ATGAA 3 cut(s) 147, 214, 381
TspRI CASTG 2 cut(s) 78, 214
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.