MD12G1203300.v1.1

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009487
Physical Location & Seq
Reverse (-)
28326317 .. 28326874
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1203300.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGCTTCTGCTAATAAGTTTCACTCATGCCCGAGCGTTCCGATGCTATTGGTTCTCTTCTTCCTCGTGATCAATATCACTCCATCCTCGAGCCAAGACACCCAGCAACTGATCGATCATATTTGCAGGCAGATGGAGGAGTACGGATTTTGCAGCCAAACCTTCAAAGAGAACTTGAAATCTCCAAACTCCGATATCGTCGCCCTCACCCAAATAACCATAGAGCGTGCCACAGACAATGCCACCAAAACGCACGACTTTATCAGGCAACTGCTTGACAACACAACCGACATAGCATTCAAAAACGCCCTCACTGCATGTGAGAATGGTTACCGTGTAGTGATGGAATCGTTCGATTCGGCTGCAGTGTCGTTTTTCCAGAAGGATTACGACAGTGTGGAGAAGGCTGAGAGGGTGACGCCGAGAGCAGAATCCAGCTGCGAAGAATCGCTTAGCACACAGCCTAATACTCAGAATCCGTTGAATGATAGAAATAGGCAGATGAGGATTTTGATTGCTATGGCTTTGGTTTCTGCGCATGAACTAAGTCAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

186

Amino Acids

20.79

Weight (kDa)

5.05

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 176 2.8e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 537
AcyI GRCGYC 1 cut(s) 419
AfaI GTAC 1 cut(s) 143
AgsI TTSAA 4 cut(s) 166, 178, 301, 484
AluBI AGCT 1 cut(s) 438
AluI AGCT 1 cut(s) 438
AlwNI CAGNNNCTG 1 cut(s) 109
Ama87I CYCGRG 2 cut(s) 31, 88
ApeKI GCWGC 3 cut(s) 153, 362, 438
AspLEI GCGC 1 cut(s) 538
AsuHPI GGTGA 2 cut(s) 199, 427
AvaI CYCGRG 2 cut(s) 31, 88
BauI CACGAG 1 cut(s) 65
BbvI GCAGC 3 cut(s) 165, 349, 425
BccI CCATC 3 cut(s) 91, 127, 337
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BclI TGATCA 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 3 cut(s) 154, 363, 439
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 3 cut(s) 155, 364, 440
BmeT110I CYCGRG 2 cut(s) 31, 88
BmsI GCATC 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 452
Bsa29I ATCGAT 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 419
Bse8I GATNNNNATC 1 cut(s) 74
BseCI ATCGAT 1 cut(s) 114
BseGI GGATG 1 cut(s) 83
BseJI GATNNNNATC 1 cut(s) 74
BseMII CTCAG 2 cut(s) 399, 485
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 3 cut(s) 165, 349, 425
BseYI CCCAGC 1 cut(s) 102
BshVI ATCGAT 1 cut(s) 114
BsiHKCI CYCGRG 2 cut(s) 31, 88
BsmI GAATGC 1 cut(s) 296
BsoBI CYCGRG 2 cut(s) 31, 88
Bsp143I GATC 3 cut(s) 69, 111, 115
Bsp1720I GCTNAGC 1 cut(s) 452
BspCNI CTCAG 2 cut(s) 400, 484
BspDI ATCGAT 1 cut(s) 114
BspMAI CTGCAG 1 cut(s) 367
BssMI GATC 3 cut(s) 69, 111, 115
BssNI GRCGYC 1 cut(s) 419
BssSI CACGAG 1 cut(s) 65
Bst2BI CACGAG 1 cut(s) 65
Bst4CI ACNGT 2 cut(s) 335, 395
Bst6I CTCTTC 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 419
BstC8I GCNNGC 2 cut(s) 128, 228
BstDEI CTNAG 4 cut(s) 408, 452, 471, 545
BstEII GGTNACC 1 cut(s) 329
BstF5I GGATG 1 cut(s) 83
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 3 cut(s) 72, 114, 118
BstMBI GATC 3 cut(s) 69, 111, 115
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNSI RCATGY 1 cut(s) 321
BstPI GGTNACC 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 3 cut(s) 165, 349, 425
Bsu15I ATCGAT 1 cut(s) 114
BsuTUI ATCGAT 1 cut(s) 114
BtsCI GGATG 1 cut(s) 83
BtsI GCAGTG 2 cut(s) 312, 372
BtsIMutI CAGTG 3 cut(s) 312, 372, 400
Cac8I GCNNGC 2 cut(s) 128, 228
CaiI CAGNNNCTG 1 cut(s) 109
CfoI GCGC 1 cut(s) 538
ClaI ATCGAT 1 cut(s) 114
CseI GACGC 1 cut(s) 427
Csp6I GTAC 1 cut(s) 142
CviAII CATG 3 cut(s) 27, 318, 539
CviJI RGCY 8 cut(s) 5, 93, 156, 362, 407, 438, 463, 524
CviKI_1 RGCY 8 cut(s) 5, 93, 156, 362, 407, 438, 463, 524
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 4 cut(s) 408, 452, 471, 545
DpnI GATC 3 cut(s) 71, 113, 117
DpnII GATC 3 cut(s) 69, 111, 115
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco32I GATATC 1 cut(s) 196
Eco88I CYCGRG 2 cut(s) 31, 88
Eco91I GGTNACC 1 cut(s) 329
EcoO65I GGTNACC 1 cut(s) 329
EcoRV GATATC 1 cut(s) 196
FaeI CATG 3 cut(s) 30, 321, 542
FaiI YATR 8 cut(s) 28, 120, 221, 293, 319, 521, 540, 556
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FatI CATG 3 cut(s) 26, 317, 538
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 154, 363, 439
FokI GGATG 1 cut(s) 70
Fsp4HI GCNGC 3 cut(s) 154, 363, 439
FspI TGCGCA 1 cut(s) 537
GlaI GCGC 1 cut(s) 537
GluI GCNGC 3 cut(s) 154, 363, 439
GsaI CCCAGC 1 cut(s) 106
HgaI GACGC 1 cut(s) 427
HhaI GCGC 1 cut(s) 538
Hin1I GRCGYC 1 cut(s) 419
Hin1II CATG 3 cut(s) 30, 321, 542
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HinfI GANTC 5 cut(s) 347, 356, 431, 446, 475
HphI GGTGA 2 cut(s) 199, 427
Hpy188I TCNGA 3 cut(s) 42, 193, 474
Hpy188III TCNNGA 2 cut(s) 67, 379
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 172, 376, 397
HpyCH4III ACNGT 2 cut(s) 335, 395
HpyCH4V TGCA 4 cut(s) 126, 153, 317, 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 4 cut(s) 408, 452, 471, 545
Hsp92I GRCGYC 1 cut(s) 419
Hsp92II CATG 3 cut(s) 30, 321, 542
HspAI GCGC 1 cut(s) 536
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 3 cut(s) 69, 111, 115
LpnPI CCDG 5 cut(s) 112, 116, 250, 392, 448
Lsp1109I GCAGC 3 cut(s) 165, 349, 425
LweI GCATC 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 329, 415
MalI GATC 3 cut(s) 71, 113, 117
MboI GATC 3 cut(s) 69, 111, 115
MboII GAAGA 3 cut(s) 49, 52, 455
MnlI CCTC 7 cut(s) 74, 97, 130, 215, 320, 405, 498
MspA1I CMGCKG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 314
NdeII GATC 3 cut(s) 69, 111, 115
NlaIII CATG 3 cut(s) 30, 321, 542
NmeAIII GCCGAG 1 cut(s) 447
NmuCI GTSAC 1 cut(s) 415
NsbI TGCGCA 1 cut(s) 537
NspI RCATGY 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 88
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 5 cut(s) 347, 356, 431, 446, 475
PkrI GCNGC 3 cut(s) 155, 364, 440
PspEI GGTNACC 1 cut(s) 329
PspFI CCCAGC 1 cut(s) 102
PspXI VCTCGAGB 1 cut(s) 88
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 438
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SatI GCNGC 3 cut(s) 154, 363, 439
Sau3AI GATC 3 cut(s) 69, 111, 115
SetI ASST 2 cut(s) 164, 440
SfaNI GCATC 1 cut(s) 33
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 88
SlaI CTCGAG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
TaaI ACNGT 2 cut(s) 335, 395
TaqI TCGA 3 cut(s) 89, 114, 354
TfiI GAWTC 5 cut(s) 347, 356, 431, 446, 475
TscAI CASTG 3 cut(s) 319, 372, 400
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 3 cut(s) 153, 362, 438
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 1 cut(s) 555
TspGWI ACGGA 2 cut(s) 159, 468
TspRI CASTG 3 cut(s) 319, 372, 400
XceI RCATGY 1 cut(s) 321
XhoI CTCGAG 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.