FvH4_6g07980

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009487
Physical Location & Seq
Forward (+)
4764255 .. 4765046
792 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07980.t1

Sequence Viewer

Length: 594 bp
ATGGCTTTTGCTAGCAAGTTTGTACTTGTTTCATCACTGTTGGTTGTGTTCCTCATGATCAACATCAAACCATCATCGAGTCAAACTACAGACGAACTGATCGATGAGATATGCCGGGCCACAGAGGACTTCGGGTTTTGCACGGGAACTTTCAATACACACTTGAAGGCCCCAAATGCCGATATAGTTGCCCTGACCCAGATAACCATAGAGGTAGCATCTGATCAGGCAACCCAAACCCTTAGCTTTATCCGGCAGACACTTACCACCACAACAGACCCGGCATTGAAGAAAGCTCTTACTGAGTGTCAGGATGCTTATGCTCTAATCACGCAGCAATATGGCTCGGCAGCGGTGGCATTTTTCCAGAAGGACTACAGTAGTGTGGAGAAGGATGAGCGCCCAACCGCTCGAGTAGAAGCGAGCTGCGAAGCGACACTCAGCGCGCCGCCGAATAATCAGCAGCACATTCTGGACGATAGAAGCAAGCAGATGAGGATTTTCATTGTCATGTCTGTGAATGCAGTGGAGACACTCCTTCGAATGAAAACAAGTGTTAATTTAGCACAGGCTGGTGCACCTGCTCCGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

198

Amino Acids

21.52

Weight (kDa)

5.02

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 147 7.5e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 589
Acc36I ACCTGC 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 410
AccII CGCG 1 cut(s) 446
AciI CCGC 3 cut(s) 353, 408, 449
AfaI GTAC 1 cut(s) 24
AgsI TTSAA 3 cut(s) 154, 166, 289
AluBI AGCT 3 cut(s) 246, 296, 426
AluI AGCT 3 cut(s) 246, 296, 426
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 1 cut(s) 524
Alw44I GTGCAC 1 cut(s) 576
Ama87I CYCGRG 1 cut(s) 411
AoxI GGCC 2 cut(s) 117, 168
ApaLI GTGCAC 1 cut(s) 576
ApeKI GCWGC 4 cut(s) 334, 350, 426, 463
AspLEI GCGC 3 cut(s) 402, 446, 448
AspS9I GGNCC 2 cut(s) 117, 169
AsuC2I CCSGG 2 cut(s) 116, 281
AsuII TTCGAA 1 cut(s) 541
AsuNHI GCTAGC 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 411
BaeGI GKGCMC 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 4 cut(s) 346, 362, 413, 475
BccI CCATC 1 cut(s) 79
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BclI TGATCA 2 cut(s) 57, 223
BcnI CCSGG 2 cut(s) 116, 281
BcoDI GTCTC 1 cut(s) 524
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 2 cut(s) 87, 376
BfoI RGCGCY 1 cut(s) 403
BfuAI ACCTGC 1 cut(s) 589
BisI GCNGC 5 cut(s) 335, 351, 427, 449, 464
BlsI GCNGC 5 cut(s) 336, 352, 428, 450, 465
Bme1390I CCNGG 2 cut(s) 116, 281
BmeT110I CYCGRG 1 cut(s) 411
BmgT120I GGNCC 2 cut(s) 117, 169
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 2 cut(s) 116, 281
BmsI GCATC 2 cut(s) 227, 304
BmtI GCTAGC 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 242
Bpu14I TTCGAA 1 cut(s) 541
BpuMI CCSGG 2 cut(s) 116, 281
Bsa29I ATCGAT 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 62
Bse8I GATNNNNATC 1 cut(s) 62
BseCI ATCGAT 1 cut(s) 102
BseGI GGATG 2 cut(s) 319, 400
BseJI GATNNNNATC 1 cut(s) 62
BseMII CTCAG 2 cut(s) 294, 454
BsePI GCGCGC 1 cut(s) 444
BseSI GKGCMC 1 cut(s) 580
BseXI GCAGC 4 cut(s) 346, 362, 413, 475
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 2 cut(s) 119, 170
BshVI ATCGAT 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 580
BsiHKCI CYCGRG 1 cut(s) 411
BsiSI CCGG 3 cut(s) 115, 253, 281
BsmAI GTCTC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 2 cut(s) 119, 170
BsoBI CYCGRG 1 cut(s) 411
Bsp119I TTCGAA 1 cut(s) 541
Bsp1286I GDGCHC 1 cut(s) 580
Bsp143I GATC 3 cut(s) 57, 99, 223
BspACI CCGC 3 cut(s) 353, 408, 449
BspANI GGCC 2 cut(s) 119, 170
BspCNI CTCAG 2 cut(s) 295, 453
BspDI ATCGAT 1 cut(s) 102
BspFNI CGCG 1 cut(s) 446
BspHI TCATGA 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 589
BspOI GCTAGC 1 cut(s) 15
BspT104I TTCGAA 1 cut(s) 541
BsrBI CCGCTC 1 cut(s) 410
BssHII GCGCGC 1 cut(s) 444
BssMI GATC 3 cut(s) 57, 99, 223
Bst4CI ACNGT 2 cut(s) 39, 380
BstBI TTCGAA 1 cut(s) 541
BstC8I GCNNGC 4 cut(s) 13, 424, 446, 488
BstDEI CTNAG 3 cut(s) 242, 303, 440
BstF5I GGATG 2 cut(s) 319, 400
BstFNI CGCG 1 cut(s) 446
BstH2I RGCGCY 1 cut(s) 403
BstHHI GCGC 3 cut(s) 402, 446, 448
BstKTI GATC 3 cut(s) 60, 102, 226
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 3 cut(s) 57, 99, 223
BstMWI GCNNNNNNNGC 2 cut(s) 176, 356
BstSCI CCNGG 2 cut(s) 114, 279
BstSFI CTRYAG 2 cut(s) 87, 376
BstSLI GKGCMC 1 cut(s) 580
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 4 cut(s) 346, 362, 413, 475
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 2 cut(s) 119, 170
BsuTUI ATCGAT 1 cut(s) 102
BtsCI GGATG 2 cut(s) 319, 400
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 2 cut(s) 35, 531
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 4 cut(s) 13, 424, 446, 488
CciI TCATGA 1 cut(s) 54
CfoI GCGC 3 cut(s) 402, 446, 448
Cfr13I GGNCC 2 cut(s) 117, 169
ClaI ATCGAT 1 cut(s) 102
Csp6I GTAC 1 cut(s) 23
CviAII CATG 2 cut(s) 55, 511
CviJI RGCY 8 cut(s) 5, 119, 170, 246, 296, 345, 426, 572
CviKI_1 RGCY 8 cut(s) 5, 119, 170, 246, 296, 345, 426, 572
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 3 cut(s) 242, 303, 440
DpnI GATC 3 cut(s) 59, 101, 225
DpnII GATC 3 cut(s) 57, 99, 223
Eco88I CYCGRG 1 cut(s) 411
EcoO109I RGGNCCY 1 cut(s) 169
FaeI CATG 2 cut(s) 58, 514
FaiI YATR 7 cut(s) 56, 112, 185, 209, 321, 342, 512
FatI CATG 2 cut(s) 54, 510
FbaI TGATCA 2 cut(s) 57, 223
Fnu4HI GCNGC 5 cut(s) 335, 351, 427, 449, 464
FokI GGATG 2 cut(s) 326, 407
Fsp4HI GCNGC 5 cut(s) 335, 351, 427, 449, 464
FspBI CTAG 1 cut(s) 12
GlaI GCGC 3 cut(s) 401, 445, 447
GluI GCNGC 5 cut(s) 335, 351, 427, 449, 464
HaeII RGCGCY 1 cut(s) 403
HaeIII GGCC 2 cut(s) 119, 170
HapII CCGG 3 cut(s) 115, 253, 281
HhaI GCGC 3 cut(s) 402, 446, 448
Hin1II CATG 2 cut(s) 58, 514
Hin6I GCGC 3 cut(s) 400, 444, 446
HinP1I GCGC 3 cut(s) 400, 444, 446
HinfI GANTC 1 cut(s) 79
HpaII CCGG 3 cut(s) 115, 253, 281
Hpy166II GTNNAC 1 cut(s) 578
Hpy188I TCNGA 2 cut(s) 223, 588
Hpy188III TCNNGA 4 cut(s) 55, 311, 367, 473
Hpy8I GTNNAC 1 cut(s) 578
HpyAV CCTTC 4 cut(s) 160, 364, 385, 548
HpyCH4III ACNGT 2 cut(s) 39, 380
HpyCH4V TGCA 3 cut(s) 141, 524, 578
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 356
HpyF3I CTNAG 3 cut(s) 242, 303, 440
Hsp92II CATG 2 cut(s) 58, 514
HspAI GCGC 3 cut(s) 400, 444, 446
Ksp22I TGATCA 2 cut(s) 57, 223
Kzo9I GATC 3 cut(s) 57, 99, 223
LmnI GCTCC 1 cut(s) 589
Lsp1109I GCAGC 4 cut(s) 346, 362, 413, 475
LweI GCATC 2 cut(s) 227, 304
MaeI CTAG 1 cut(s) 12
MalI GATC 3 cut(s) 59, 101, 225
MbiI CCGCTC 1 cut(s) 410
MboI GATC 3 cut(s) 57, 99, 223
MboII GAAGA 1 cut(s) 301
MhlI GDGCHC 1 cut(s) 580
MluCI AATT 1 cut(s) 559
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 4 cut(s) 62, 118, 205, 489
MseI TTAA 1 cut(s) 558
MslI CAYNNNNRTG 2 cut(s) 509, 515
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 3 cut(s) 115, 253, 281
MspR9I CCNGG 2 cut(s) 116, 281
Mva1269I GAATGC 1 cut(s) 526
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 2 cut(s) 176, 356
NciI CCSGG 2 cut(s) 116, 281
NdeII GATC 3 cut(s) 57, 99, 223
NheI GCTAGC 1 cut(s) 11
NlaIII CATG 2 cut(s) 58, 514
NlaIV GGNNCC 1 cut(s) 171
NmeAIII GCCGAG 1 cut(s) 326
NspV TTCGAA 1 cut(s) 541
PaeR7I CTCGAG 1 cut(s) 411
PagI TCATGA 1 cut(s) 54
PaqCI CACCTGC 1 cut(s) 589
PauI GCGCGC 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 526
PkrI GCNGC 5 cut(s) 336, 352, 428, 450, 465
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 2 cut(s) 117, 169
PspXI VCTCGAGB 1 cut(s) 411
PsrI GAACNNNNNNTAC 2 cut(s) 139, 171
PteI GCGCGC 1 cut(s) 444
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
RseI CAYNNNNRTG 2 cut(s) 509, 515
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 5 cut(s) 335, 351, 427, 449, 464
Sau3AI GATC 3 cut(s) 57, 99, 223
Sau96I GGNCC 2 cut(s) 117, 169
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 116, 281
SduI GDGCHC 1 cut(s) 580
SetI ASST 5 cut(s) 216, 248, 298, 428, 583
SfaNI GCATC 2 cut(s) 227, 304
SfcI CTRYAG 2 cut(s) 87, 376
Sfr274I CTCGAG 1 cut(s) 411
SfuI TTCGAA 1 cut(s) 541
SlaI CTCGAG 1 cut(s) 411
SmiMI CAYNNNNRTG 2 cut(s) 509, 515
SmlI CTYRAG 1 cut(s) 411
SmoI CTYRAG 1 cut(s) 411
Sse9I AATT 1 cut(s) 559
SsiI CCGC 3 cut(s) 353, 408, 449
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 2 cut(s) 114, 279
TaaI ACNGT 2 cut(s) 39, 380
TaqI TCGA 4 cut(s) 77, 102, 412, 541
TasI AATT 1 cut(s) 559
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 451
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TscAI CASTG 2 cut(s) 42, 531
TseI GCWGC 4 cut(s) 334, 350, 426, 463
TspDTI ATGAA 3 cut(s) 21, 493, 560
TspRI CASTG 2 cut(s) 42, 531
VneI GTGCAC 1 cut(s) 576
XhoI CTCGAG 1 cut(s) 411
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.