FvH4_6g08020

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009487
Physical Location & Seq
Forward (+)
4778563 .. 4779312
750 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08020.t1

Sequence Viewer

Length: 588 bp
ATGGCTTCTGCTAGTAAGTCTGTGCTTGTTTCATTACTTTTGGTGGTGTTCCTCACGATCAATGTCGAACCATCACTGAGTCAAAGCACACAGCAACTGATCGAGAAGATATGCCGGTCCACAGAGGACTATGGCTTTTGCAGGACAACATTCCAAGCACACTTGAAGTCCCCAAATGCTGATATAGTCGCTCTAACACAGATAACCGTAGAGGTTGCAGTAGATCACTCAACCAAAACTCACAACTTTATCAGGCAGACCATTGCAACAACAAAAGACCCGGTATTGAAGAAAGCTCTCTCCGAGTGTGAGAATGCTTATGGTCTAATCATGCAGGCATTTGACTTGGCAGCGGTGTCGTTTTTCAGCAGGGACTACAGCAGTGTGGAGAAGGAGGAGCGCATAACGCCGCGAGCAGAAGCGAGCTGCAGGGCAACACTCACTACGCCACCGATCAGTAAGCAGCATGGCATTCTGGACGAAAGAAGCAGGGAAATGAGGATTTTCATTGTCATGTCTCTGAATGCTGTGCAGGATCTCCTTCGACCAAGGTCTTTAGGACCTGCTGCTGCACCTGCTCTGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

196

Amino Acids

21.51

Weight (kDa)

7.7

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 144 1.9e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 583
Acc36I ACCTGC 2 cut(s) 571, 583
AccII CGCG 1 cut(s) 412
AciI CCGC 2 cut(s) 353, 410
AclWI GGATC 1 cut(s) 543
AgsI TTSAA 2 cut(s) 166, 289
AluBI AGCT 2 cut(s) 296, 426
AluI AGCT 2 cut(s) 296, 426
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 1 cut(s) 543
AlwNI CAGNNNCTG 1 cut(s) 97
ApeKI GCWGC 5 cut(s) 350, 426, 463, 566, 569
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 2 cut(s) 117, 560
AsuC2I CCSGG 1 cut(s) 281
AvaII GGWCC 2 cut(s) 117, 560
BbvI GCAGC 5 cut(s) 362, 413, 475, 553, 556
BccI CCATC 1 cut(s) 79
BcgI CGANNNNNNTGC 2 cut(s) 339, 373
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 2 cut(s) 376, 427
BfuAI ACCTGC 2 cut(s) 571, 583
BisI GCNGC 6 cut(s) 351, 410, 427, 464, 567, 570
BlsI GCNGC 6 cut(s) 352, 411, 428, 465, 568, 571
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 2 cut(s) 117, 560
BmgT120I GGNCC 2 cut(s) 117, 560
BmrFI CCNGG 1 cut(s) 281
BoxI GACNNNNGTC 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 548
Bse118I RCCGGY 1 cut(s) 114
Bse3DI GCAATG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 548
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 68
BseRI GAGGAG 1 cut(s) 410
BseXI GCAGC 5 cut(s) 362, 413, 475, 553, 556
BsgI GTGCAG 2 cut(s) 551, 555
Bsh1236I CGCG 1 cut(s) 412
BsiSI CCGG 2 cut(s) 115, 281
BslFI GGGAC 2 cut(s) 154, 386
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 2 cut(s) 154, 386
BsmI GAATGC 3 cut(s) 319, 471, 529
Bsp143I GATC 5 cut(s) 57, 99, 223, 453, 535
BspACI CCGC 2 cut(s) 353, 410
BspCNI CTCAG 1 cut(s) 69
BspFNI CGCG 1 cut(s) 412
BspMAI CTGCAG 1 cut(s) 431
BspMI ACCTGC 2 cut(s) 571, 583
BspPI GGATC 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 5 cut(s) 57, 99, 223, 453, 535
BssT1I CCWWGG 1 cut(s) 548
Bst4CI ACNGT 1 cut(s) 208
BstC8I GCNNGC 3 cut(s) 336, 414, 424
BstDEI CTNAG 1 cut(s) 77
BstFNI CGCG 1 cut(s) 412
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 5 cut(s) 60, 102, 226, 456, 538
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 5 cut(s) 57, 99, 223, 453, 535
BstMWI GCNNNNNNNGC 2 cut(s) 406, 575
BstPAI GACNNNNGTC 1 cut(s) 550
BstSCI CCNGG 1 cut(s) 279
BstSFI CTRYAG 2 cut(s) 376, 427
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 5 cut(s) 362, 413, 475, 553, 556
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 2 cut(s) 74, 388
BveI ACCTGC 2 cut(s) 571, 583
Cac8I GCNNGC 3 cut(s) 336, 414, 424
CaiI CAGNNNCTG 1 cut(s) 97
CfoI GCGC 1 cut(s) 402
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 2 cut(s) 117, 560
CviAII CATG 3 cut(s) 331, 467, 514
CviJI RGCY 4 cut(s) 5, 135, 296, 426
CviKI_1 RGCY 4 cut(s) 5, 135, 296, 426
DdeI CTNAG 1 cut(s) 77
DpnI GATC 5 cut(s) 59, 101, 225, 455, 537
DpnII GATC 5 cut(s) 57, 99, 223, 453, 535
Eco130I CCWWGG 1 cut(s) 548
Eco47I GGWCC 2 cut(s) 117, 560
EcoO109I RGGNCCY 1 cut(s) 560
EcoT14I CCWWGG 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 548
FaeI CATG 3 cut(s) 334, 470, 517
FaiI YATR 8 cut(s) 112, 132, 185, 321, 332, 404, 468, 515
FaqI GGGAC 2 cut(s) 154, 386
FatI CATG 3 cut(s) 330, 466, 513
Fnu4HI GCNGC 6 cut(s) 351, 410, 427, 464, 567, 570
Fsp4HI GCNGC 6 cut(s) 351, 410, 427, 464, 567, 570
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 401
GluI GCNGC 6 cut(s) 351, 410, 427, 464, 567, 570
HapII CCGG 2 cut(s) 115, 281
HhaI GCGC 1 cut(s) 402
Hin1II CATG 3 cut(s) 334, 470, 517
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HinfI GANTC 1 cut(s) 79
HpaII CCGG 2 cut(s) 115, 281
Hpy166II GTNNAC 1 cut(s) 120
Hpy188I TCNGA 2 cut(s) 304, 522
Hpy188III TCNNGA 3 cut(s) 55, 103, 476
Hpy8I GTNNAC 1 cut(s) 120
HpyAV CCTTC 2 cut(s) 385, 551
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4V TGCA 7 cut(s) 141, 218, 266, 334, 429, 532, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 406, 575
HpyF3I CTNAG 1 cut(s) 77
Hsp92II CATG 3 cut(s) 334, 470, 517
HspAI GCGC 1 cut(s) 400
Kzo9I GATC 5 cut(s) 57, 99, 223, 453, 535
LmnI GCTCC 1 cut(s) 397
Lsp1109I GCAGC 5 cut(s) 362, 413, 475, 553, 556
MaeI CTAG 1 cut(s) 12
MalI GATC 5 cut(s) 59, 101, 225, 455, 537
MboI GATC 5 cut(s) 57, 99, 223, 453, 535
MboII GAAGA 2 cut(s) 118, 301
MflI RGATCY 1 cut(s) 535
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 5 cut(s) 62, 118, 205, 388, 492
MslI CAYNNNNRTG 1 cut(s) 512
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 115, 281
MspR9I CCNGG 1 cut(s) 281
Mva1269I GAATGC 3 cut(s) 319, 471, 529
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 2 cut(s) 406, 575
NciI CCSGG 1 cut(s) 281
NdeII GATC 5 cut(s) 57, 99, 223, 453, 535
NlaIII CATG 3 cut(s) 334, 470, 517
PaqCI CACCTGC 1 cut(s) 583
PctI GAATGC 3 cut(s) 319, 471, 529
PkrI GCNGC 6 cut(s) 352, 411, 428, 465, 568, 571
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PpuMI RGGWCCY 1 cut(s) 560
PshAI GACNNNNGTC 1 cut(s) 550
Psp5II RGGWCCY 1 cut(s) 560
PspPI GGNCC 2 cut(s) 117, 560
PspPPI RGGWCCY 1 cut(s) 560
PstI CTGCAG 1 cut(s) 431
PstNI CAGNNNCTG 1 cut(s) 97
PsuI RGATCY 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 512
SatI GCNGC 6 cut(s) 351, 410, 427, 464, 567, 570
Sau3AI GATC 5 cut(s) 57, 99, 223, 453, 535
Sau96I GGNCC 2 cut(s) 117, 560
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 281
SetI ASST 6 cut(s) 216, 298, 428, 554, 565, 577
SfcI CTRYAG 2 cut(s) 376, 427
SinI GGWCC 2 cut(s) 117, 560
SmiMI CAYNNNNRTG 1 cut(s) 512
SsiI CCGC 2 cut(s) 353, 410
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 1 cut(s) 208
TaqI TCGA 3 cut(s) 66, 102, 544
TauI GCSGC 1 cut(s) 412
TscAI CASTG 2 cut(s) 81, 388
TseI GCWGC 5 cut(s) 350, 426, 463, 566, 569
TspDTI ATGAA 2 cut(s) 21, 496
TspRI CASTG 2 cut(s) 81, 388
VpaK11BI GGWCC 2 cut(s) 117, 560
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.