MD12G1203400.v1.1

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009487
Physical Location & Seq
Reverse (-)
28346193 .. 28346756
564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1203400.v1.1.491

Sequence Viewer

Length: 564 bp
ATGAAAATGGCTTCTGCTTCATTCCCAGGTGTTCCAGTATTACTGGTTCTCTTCTTCCTCGTGATCAATATCACTCCATCGTTGACTCAAGACACCGAACAACTGATCGAGCGTATCTGCAGGCAAATGGAGCAGTATGGATTTTGCAGCCAAACCTTTAAAGAAAACCTGAAGTCTCCTAATGCTGATATCTTGGCCCTCACCCAGATTACCGTAGAGCGTGCTTTAGACAATGCCACCCAAACACACGACTTCATTAGGCAACTGCTTGAAAGCACAACCGACACAGCACTCAAAAGCGCTCTGGAAACATGCGAGTATAGTTACCGTTTGGTGATGGAGGCGTTTCAATCGGCTGCGGTGGCGTTTTTCCAGAAGGATTACGACAGCGTAGAGAAGTCTGAGAGTGGGACGCCGAGAGCAGAAGCTAGCTGCGCAGACTCACTGAGCAAGATGCCGCCAAATACTGCGAACCCACTGACTGAGAGAAATAATCAGATGAGGATTCTACTTGCCATGGCTTTGGTTTCAGTGCATGAACTAAAGTCTACCCAGAACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

188

Amino Acids

20.88

Weight (kDa)

4.96

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 175 3e-15 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 436
AccI GTMKAC 1 cut(s) 548
AciI CCGC 2 cut(s) 359, 458
AcuI CTGAAG 1 cut(s) 191
AcyI GRCGYC 1 cut(s) 413
AfeI AGCGCT 1 cut(s) 301
AgsI TTSAA 2 cut(s) 272, 350
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 2 cut(s) 428, 432
AluI AGCT 2 cut(s) 428, 432
Alw26I GTCTC 1 cut(s) 180
Aor51HI AGCGCT 1 cut(s) 301
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 3 cut(s) 147, 356, 432
AspLEI GCGC 2 cut(s) 302, 437
AspS9I GGNCC 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 193, 346
AsuNHI GCTAGC 1 cut(s) 428
BauI CACGAG 1 cut(s) 59
BbvI GCAGC 3 cut(s) 159, 343, 419
BccI CCATC 2 cut(s) 85, 331
BciT130I CCWGG 1 cut(s) 27
BclI TGATCA 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 2 cut(s) 429, 562
BfmI CTRYAG 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 303
BisI GCNGC 4 cut(s) 148, 357, 433, 458
BlsI GCNGC 4 cut(s) 149, 358, 434, 459
Bme1390I CCNGG 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 1 cut(s) 444
BmtI GCTAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 25, 516
Bse1I ACTGG 2 cut(s) 35, 48
Bse8I GATNNNNATC 1 cut(s) 68
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 25, 516
BseJI GATNNNNATC 1 cut(s) 68
BseMII CTCAG 3 cut(s) 393, 437, 474
BseNI ACTGG 2 cut(s) 35, 48
BseXI GCAGC 3 cut(s) 159, 343, 419
BshFI GGCC 1 cut(s) 197
BslFI GGGAC 1 cut(s) 424
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 424
BsnI GGCC 1 cut(s) 197
Bsp143I GATC 2 cut(s) 63, 105
Bsp19I CCATGG 1 cut(s) 516
BspACI CCGC 2 cut(s) 359, 458
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 3 cut(s) 394, 438, 475
BspMAI CTGCAG 1 cut(s) 122
BspOI GCTAGC 1 cut(s) 432
BsrI ACTGG 2 cut(s) 35, 48
BssECI CCNNGG 2 cut(s) 25, 516
BssMI GATC 2 cut(s) 63, 105
BssNI GRCGYC 1 cut(s) 413
BssSI CACGAG 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 516
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 214, 329
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 122, 222, 430
BstDEI CTNAG 3 cut(s) 402, 446, 483
BstDSI CCRYGG 1 cut(s) 516
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 2 cut(s) 302, 437
BstKTI GATC 2 cut(s) 66, 108
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 2 cut(s) 63, 105
BstMWI GCNNNNNNNGC 3 cut(s) 130, 362, 434
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 25
BstSFI CTRYAG 1 cut(s) 118
BstV1I GCAGC 3 cut(s) 159, 343, 419
BstXI CCANNNNNNTGG 1 cut(s) 523
BsuRI GGCC 1 cut(s) 197
BtgI CCRYGG 1 cut(s) 516
BtsIMutI CAGTG 3 cut(s) 443, 476, 537
Cac8I GCNNGC 3 cut(s) 122, 222, 430
CfoI GCGC 2 cut(s) 302, 437
Cfr13I GGNCC 1 cut(s) 196
CseI GACGC 1 cut(s) 421
CviAII CATG 3 cut(s) 312, 517, 536
CviJI RGCY 7 cut(s) 11, 150, 197, 356, 428, 432, 521
CviKI_1 RGCY 7 cut(s) 11, 150, 197, 356, 428, 432, 521
DdeI CTNAG 3 cut(s) 402, 446, 483
DpnI GATC 2 cut(s) 65, 107
DpnII GATC 2 cut(s) 63, 105
DraI TTTAAA 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 516
Eco32I GATATC 1 cut(s) 190
Eco47III AGCGCT 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 191
EcoRII CCWGG 1 cut(s) 25
EcoRV GATATC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 3 cut(s) 315, 520, 539
FaiI YATR 5 cut(s) 138, 313, 321, 518, 537
FaqI GGGAC 1 cut(s) 424
FatI CATG 3 cut(s) 311, 516, 535
FbaI TGATCA 1 cut(s) 63
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 4 cut(s) 148, 357, 433, 458
Fsp4HI GCNGC 4 cut(s) 148, 357, 433, 458
FspBI CTAG 2 cut(s) 429, 562
FspI TGCGCA 1 cut(s) 436
GlaI GCGC 2 cut(s) 301, 436
GluI GCNGC 4 cut(s) 148, 357, 433, 458
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 1 cut(s) 197
HgaI GACGC 1 cut(s) 421
HhaI GCGC 2 cut(s) 302, 437
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 3 cut(s) 315, 520, 539
Hin6I GCGC 2 cut(s) 300, 435
HinP1I GCGC 2 cut(s) 300, 435
HincII GTYRAC 1 cut(s) 84
HindII GTYRAC 1 cut(s) 84
HinfI GANTC 3 cut(s) 85, 440, 505
HphI GGTGA 2 cut(s) 193, 346
Hpy166II GTNNAC 2 cut(s) 84, 549
Hpy188I TCNGA 2 cut(s) 403, 498
Hpy188III TCNNGA 4 cut(s) 61, 89, 305, 373
Hpy8I GTNNAC 2 cut(s) 84, 549
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 2 cut(s) 214, 329
HpyCH4V TGCA 3 cut(s) 120, 147, 535
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 362, 434
HpyF3I CTNAG 3 cut(s) 402, 446, 483
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 3 cut(s) 315, 520, 539
HspAI GCGC 2 cut(s) 300, 435
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 2 cut(s) 63, 105
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 9 cut(s) 12, 29, 39, 48, 106, 182, 218, 290, 386
Lsp1109I GCAGC 3 cut(s) 159, 343, 419
LweI GCATC 1 cut(s) 444
MaeI CTAG 2 cut(s) 429, 562
MaeIII GTNAC 1 cut(s) 323
MalI GATC 2 cut(s) 65, 107
MboI GATC 2 cut(s) 63, 105
MboII GAAGA 2 cut(s) 43, 46
MlyI GAGTC 2 cut(s) 79, 434
MnlI CCTC 4 cut(s) 68, 209, 334, 495
MseI TTAA 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 3 cut(s) 130, 362, 434
NcoI CCATGG 1 cut(s) 516
NdeII GATC 2 cut(s) 63, 105
NheI GCTAGC 1 cut(s) 428
NlaIII CATG 3 cut(s) 315, 520, 539
NmeAIII GCCGAG 1 cut(s) 441
NsbI TGCGCA 1 cut(s) 436
NspI RCATGY 1 cut(s) 315
PfeI GAWTC 1 cut(s) 505
PkrI GCNGC 4 cut(s) 149, 358, 434, 459
PleI GAGTC 2 cut(s) 79, 434
PpsI GAGTC 2 cut(s) 79, 434
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspPI GGNCC 1 cut(s) 196
PsrI GAACNNNNNNTAC 2 cut(s) 30, 62
PstI CTGCAG 1 cut(s) 122
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 4 cut(s) 148, 357, 433, 458
Sau3AI GATC 2 cut(s) 63, 105
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 2 cut(s) 79, 434
ScrFI CCNGG 1 cut(s) 27
SetI ASST 5 cut(s) 31, 158, 171, 430, 434
SfaNI GCATC 1 cut(s) 444
SfcI CTRYAG 1 cut(s) 118
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
SsiI CCGC 2 cut(s) 359, 458
SspMI CTAG 2 cut(s) 429, 562
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 2 cut(s) 214, 329
TaqI TCGA 1 cut(s) 108
TauI GCSGC 1 cut(s) 460
TfiI GAWTC 1 cut(s) 505
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 3 cut(s) 450, 483, 537
TseI GCWGC 3 cut(s) 147, 356, 432
TspDTI ATGAA 4 cut(s) 9, 17, 244, 552
TspRI CASTG 3 cut(s) 450, 483, 537
XceI RCATGY 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 548
XspI CTAG 2 cut(s) 429, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.