MD12G1203500.v1.1

Inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009487
Physical Location & Seq
Reverse (-)
28358479 .. 28359039
561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1203500.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGCATCTGCATCATGCCCGCGTGTTCCAATATTGCTGGTTATTCTCTTCTTCCTCATGATCAACATCACTCCATCTTTTACTCAAGACACCCAGCAGTTGATTGAGCGTATCTGCAGATCAACGGAGCAGTACGGATTTTGCACCCAAACCTTCAAAGAAAACCTGAAATCCCCAGATGCTGATATCGTCGCCCTCACCCAAATAACCATAGAGCGGTCCGTAGACAATGCTACTCAAACACACAACTTCATCAGGCAAACGATTGATAGCACAAACGACCCAGCAGTCAAAAGCGCTTTGGAAACATGCGAGTATAGTTACGGTTTAGTGATGGAGGCATTTGACTCGGCTGCCGTGGCGTTTTTCCAGAAGGATTACGACACCGTAGAGAAGTCTGAGAGTGGGACGCCGAGAGCAGAAGCCAGCTGTGAAGACTCGCTGAGCAAGACGTCGCCAAATACTGTGAACCCACTGACTGACAGAAATAATCAGATGAGGATTCTGATTGCCATGTCTTTGAATGCTGTGCATGAACTAAAGTCTACCCAGCACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

187

Amino Acids

20.75

Weight (kDa)

4.82

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 174 3.1e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
AatII GACGTC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 217
AccI GTMKAC 2 cut(s) 225, 545
AccII CGCG 1 cut(s) 22
AciI CCGC 2 cut(s) 20, 217
AcyI GRCGYC 2 cut(s) 410, 452
AfaI GTAC 1 cut(s) 134
AfeI AGCGCT 1 cut(s) 298
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 2 cut(s) 157, 523
AluBI AGCT 1 cut(s) 429
AluI AGCT 1 cut(s) 429
AlwNI CAGNNNCTG 1 cut(s) 182
Aor51HI AGCGCT 1 cut(s) 298
ApeKI GCWGC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 219
AsuHPI GGTGA 1 cut(s) 190
AvaII GGWCC 1 cut(s) 219
BbsI GAAGAC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 340
BccI CCATC 2 cut(s) 82, 328
BceAI ACGGC 1 cut(s) 341
BcgI CGANNNNNNTGC 2 cut(s) 330, 364
BclI TGATCA 1 cut(s) 60
BfaI CTAG 1 cut(s) 559
BfmI CTRYAG 1 cut(s) 115
BfoI RGCGCY 1 cut(s) 300
BisI GCNGC 1 cut(s) 354
BlpI GCTNAGC 1 cut(s) 443
BlsI GCNGC 1 cut(s) 355
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 219
BmsI GCATC 3 cut(s) 14, 20, 169
BpiI GAAGAC 1 cut(s) 441
Bpu1102I GCTNAGC 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 69
BsaBI GATNNNNATC 1 cut(s) 65
BsaHI GRCGYC 2 cut(s) 410, 452
BsaJI CCNNGG 1 cut(s) 357
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse8I GATNNNNATC 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 357
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 390, 434
BseXI GCAGC 1 cut(s) 340
BseYI CCCAGC 3 cut(s) 93, 283, 549
Bsh1236I CGCG 1 cut(s) 22
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 1 cut(s) 216
BsmFI GGGAC 1 cut(s) 421
BsmI GAATGC 1 cut(s) 529
Bsp143I GATC 2 cut(s) 60, 119
Bsp1720I GCTNAGC 1 cut(s) 443
BspACI CCGC 2 cut(s) 20, 217
BspCNI CTCAG 2 cut(s) 391, 435
BspFNI CGCG 1 cut(s) 22
BspHI TCATGA 1 cut(s) 57
BspMAI CTGCAG 1 cut(s) 119
BsrBI CCGCTC 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 2 cut(s) 60, 119
BssNI GRCGYC 2 cut(s) 410, 452
Bst4CI ACNGT 3 cut(s) 326, 388, 466
Bst6I CTCTTC 1 cut(s) 53
BstACI GRCGYC 2 cut(s) 410, 452
BstC8I GCNNGC 2 cut(s) 20, 427
BstDEI CTNAG 2 cut(s) 399, 443
BstDSI CCRYGG 1 cut(s) 357
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 2 cut(s) 63, 122
BstMBI GATC 2 cut(s) 60, 119
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNSI RCATGY 1 cut(s) 312
BstSFI CTRYAG 1 cut(s) 115
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 441
BtgI CCRYGG 1 cut(s) 357
BtsIMutI CAGTG 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 20, 427
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 1 cut(s) 57
CfoI GCGC 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 219
CpoI CGGWCCG 1 cut(s) 219
CseI GACGC 1 cut(s) 418
Csp6I GTAC 1 cut(s) 133
CspI CGGWCCG 1 cut(s) 219
CviAII CATG 5 cut(s) 15, 58, 309, 514, 533
CviJI RGCY 3 cut(s) 353, 425, 429
CviKI_1 RGCY 3 cut(s) 353, 425, 429
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 2 cut(s) 399, 443
DpnI GATC 2 cut(s) 62, 121
DpnII GATC 2 cut(s) 60, 119
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 219
Eco47III AGCGCT 1 cut(s) 298
EcoRV GATATC 1 cut(s) 187
FaeI CATG 5 cut(s) 18, 61, 312, 517, 536
FaiI YATR 7 cut(s) 16, 59, 212, 310, 318, 515, 534
FaqI GGGAC 1 cut(s) 421
FatI CATG 5 cut(s) 14, 57, 308, 513, 532
FauI CCCGC 1 cut(s) 27
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 2 cut(s) 225, 545
Fnu4HI GCNGC 1 cut(s) 354
Fsp4HI GCNGC 1 cut(s) 354
FspBI CTAG 1 cut(s) 559
GlaI GCGC 1 cut(s) 298
GluI GCNGC 1 cut(s) 354
GsaI CCCAGC 3 cut(s) 97, 287, 553
HaeII RGCGCY 1 cut(s) 300
HgaI GACGC 1 cut(s) 418
HhaI GCGC 1 cut(s) 299
Hin1I GRCGYC 2 cut(s) 410, 452
Hin1II CATG 5 cut(s) 18, 61, 312, 517, 536
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 3 cut(s) 347, 437, 502
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 3 cut(s) 226, 469, 546
Hpy188I TCNGA 3 cut(s) 400, 495, 507
Hpy188III TCNNGA 3 cut(s) 58, 86, 370
Hpy8I GTNNAC 3 cut(s) 226, 469, 546
Hpy99I CGWCG 2 cut(s) 194, 457
HpyAV CCTTC 2 cut(s) 163, 367
HpyCH4III ACNGT 3 cut(s) 326, 388, 466
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 4 cut(s) 11, 117, 144, 532
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 2 cut(s) 399, 443
HpySE526I ACGT 1 cut(s) 452
Hsp92I GRCGYC 2 cut(s) 410, 452
Hsp92II CATG 5 cut(s) 18, 61, 312, 517, 536
HspAI GCGC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 2 cut(s) 60, 119
LmnI GCTCC 1 cut(s) 127
LpnPI CCDG 8 cut(s) 23, 107, 179, 189, 241, 297, 383, 439
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 3 cut(s) 14, 20, 169
MaeI CTAG 1 cut(s) 559
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 1 cut(s) 320
MalI GATC 2 cut(s) 62, 121
MbiI CCGCTC 1 cut(s) 217
MboI GATC 2 cut(s) 60, 119
MboII GAAGA 3 cut(s) 40, 43, 446
MlyI GAGTC 2 cut(s) 341, 431
MnlI CCTC 4 cut(s) 65, 206, 331, 492
MspA1I CMGCKG 1 cut(s) 429
Mva1269I GAATGC 1 cut(s) 529
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 1 cut(s) 359
NdeII GATC 2 cut(s) 60, 119
NlaIII CATG 5 cut(s) 18, 61, 312, 517, 536
NmeAIII GCCGAG 2 cut(s) 329, 438
NspI RCATGY 1 cut(s) 312
PagI TCATGA 1 cut(s) 57
PctI GAATGC 1 cut(s) 529
PfeI GAWTC 1 cut(s) 502
PkrI GCNGC 1 cut(s) 355
PleI GAGTC 2 cut(s) 341, 431
PpsI GAGTC 2 cut(s) 341, 431
PspFI CCCAGC 3 cut(s) 93, 283, 549
PspPI GGNCC 1 cut(s) 219
PstI CTGCAG 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 429
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
Rsr2I CGGWCCG 1 cut(s) 219
RsrII CGGWCCG 1 cut(s) 219
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 2 cut(s) 60, 119
Sau96I GGNCC 1 cut(s) 219
SchI GAGTC 2 cut(s) 341, 431
SetI ASST 4 cut(s) 155, 168, 431, 455
SfaNI GCATC 3 cut(s) 14, 20, 169
SfcI CTRYAG 1 cut(s) 115
SinI GGWCC 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SsiI CCGC 2 cut(s) 20, 217
SspI AATATT 1 cut(s) 33
SspMI CTAG 1 cut(s) 559
TaaI ACNGT 3 cut(s) 326, 388, 466
TaiI ACGT 1 cut(s) 455
TfiI GAWTC 1 cut(s) 502
TscAI CASTG 1 cut(s) 480
TseI GCWGC 1 cut(s) 353
TspDTI ATGAA 2 cut(s) 241, 549
TspGWI ACGGA 3 cut(s) 140, 150, 211
TspRI CASTG 1 cut(s) 480
VpaK11BI GGWCC 1 cut(s) 219
XceI RCATGY 1 cut(s) 312
XmiI GTMKAC 2 cut(s) 225, 545
XspI CTAG 1 cut(s) 559
ZraI GACGTC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.