AT1G09370

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009487
Physical Location & Seq
Forward (+)
3024877 .. 3025539
663 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09370.1

Sequence Viewer

Length: 531 bp
ATGAAAATGCATCACATTCTCTCGATAGTTTTGGCAATTATACTCGCTTCCTCCGTTGCAGAAGCTTTTCTCGATGAAAAAACTCAACAAAGGGTCAACGGAATCTGCAAGCAGACAATGGATACCAGATTTTGCAGTAGTCTCTTGATCAAGAACCTGAATACTTTTCCCGCAAGTAACAAAGAAATCATGAACGTGACAGTAAGCGAAGCTGAAAGGTTCGCGGCCAACACTTATTTCTTCATCAGCACGCTTCTCAGGAATGCTGGAGACGAGAGGCCTGATCTTCAAGCGTGCGCCGAAGCTTACGCCATTGTGAACTCGGCGTTCACGAAAGCTGTAACCTTCTTCAAGCAAGCTTACTACAGTAAGATTGTCAATATCGAAAAGAAAGTTTCTATGGCTGTTGATATCTGTAAGACAGATTTCAATGTGCTCGGTTATCAGATCAATCCGTTGATTGAGAAAAACAGACAGACGAAGATCTTACTATCCATGGAACAGATTGTTAGTCATATGGTTTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

176

Amino Acids

19.8

Weight (kDa)

8.66

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 175 3.3e-09 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 224
AciI CCGC 2 cut(s) 171, 224
AcoI YGGCCR 1 cut(s) 225
AgsI TTSAA 3 cut(s) 290, 352, 430
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 5 cut(s) 65, 212, 305, 338, 359
AluI AGCT 5 cut(s) 65, 212, 305, 338, 359
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 2 cut(s) 146, 264
AoxI GGCC 2 cut(s) 225, 278
Asp700I GAANNNNTTC 1 cut(s) 66
AspLEI GCGC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 438
BciVI GTATCC 1 cut(s) 115
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 146, 264
BfmI CTRYAG 1 cut(s) 364
BfuI GTATCC 1 cut(s) 115
BglII AGATCT 1 cut(s) 483
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmsI GCATC 1 cut(s) 19
BpmI CTGGAG 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 495
BseDI CCNNGG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 271
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 2 cut(s) 227, 280
BsiHKAI GWGCWC 1 cut(s) 438
BsmAI GTCTC 2 cut(s) 146, 264
BsmBI CGTCTC 1 cut(s) 264
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 2 cut(s) 227, 280
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 4 cut(s) 147, 283, 447, 483
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 2 cut(s) 171, 224
BspANI GGCC 2 cut(s) 227, 280
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 1 cut(s) 224
BspHI TCATGA 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 4 cut(s) 147, 283, 447, 483
BssT1I CCWWGG 1 cut(s) 495
Bst4CI ACNGT 2 cut(s) 202, 368
BstC8I GCNNGC 4 cut(s) 110, 251, 295, 357
BstDEI CTNAG 1 cut(s) 257
BstDSI CCRYGG 1 cut(s) 495
BstFNI CGCG 1 cut(s) 224
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 4 cut(s) 150, 286, 450, 486
BstMAI GTCTC 2 cut(s) 146, 264
BstMBI GATC 4 cut(s) 147, 283, 447, 483
BstSFI CTRYAG 1 cut(s) 364
BstUI CGCG 1 cut(s) 224
BstX2I RGATCY 1 cut(s) 483
BstYI RGATCY 1 cut(s) 483
BsuI GTATCC 1 cut(s) 115
BsuRI GGCC 2 cut(s) 227, 280
BtgI CCRYGG 1 cut(s) 495
Cac8I GCNNGC 4 cut(s) 110, 251, 295, 357
CciI TCATGA 1 cut(s) 189
CfoI GCGC 1 cut(s) 299
CviAII CATG 2 cut(s) 190, 496
CviJI RGCY 8 cut(s) 65, 212, 227, 280, 305, 338, 359, 404
CviKI_1 RGCY 8 cut(s) 65, 212, 227, 280, 305, 338, 359, 404
DdeI CTNAG 1 cut(s) 257
DpnI GATC 4 cut(s) 149, 285, 449, 485
DpnII GATC 4 cut(s) 147, 283, 447, 483
EaeI YGGCCR 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 495
Eco147I AGGCCT 1 cut(s) 280
Eco32I GATATC 1 cut(s) 412
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 495
Esp3I CGTCTC 1 cut(s) 264
FaeI CATG 2 cut(s) 193, 499
FaiI YATR 7 cut(s) 41, 191, 401, 497, 516, 518, 529
FatI CATG 2 cut(s) 189, 495
FauI CCCGC 1 cut(s) 178
FauNDI CATATG 1 cut(s) 516
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 225
GlaI GCGC 1 cut(s) 298
GluI GCNGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 2 cut(s) 227, 280
HhaI GCGC 1 cut(s) 299
Hin1II CATG 2 cut(s) 193, 499
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 3 cut(s) 63, 303, 357
HinfI GANTC 1 cut(s) 102
Hpy166II GTNNAC 3 cut(s) 97, 319, 330
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 7 cut(s) 22, 71, 145, 151, 190, 259, 331
Hpy8I GTNNAC 3 cut(s) 97, 319, 330
HpyAV CCTTC 1 cut(s) 355
HpyCH4III ACNGT 2 cut(s) 202, 368
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 4 cut(s) 10, 59, 108, 135
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 2 cut(s) 193, 499
HspAI GCGC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 4 cut(s) 147, 283, 447, 483
LpnPI CCDG 5 cut(s) 139, 170, 244, 252, 294
LweI GCATC 1 cut(s) 19
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 3 cut(s) 176, 196, 340
MalI GATC 4 cut(s) 149, 285, 449, 485
MboI GATC 4 cut(s) 147, 283, 447, 483
MboII GAAGA 5 cut(s) 232, 278, 340, 493, 516
MflI RGATCY 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 1 cut(s) 36
MnlI CCTC 2 cut(s) 61, 270
Mph1103I ATGCAT 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 194
Mva1269I GAATGC 1 cut(s) 268
MvnI CGCG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 495
NdeI CATATG 1 cut(s) 516
NdeII GATC 4 cut(s) 147, 283, 447, 483
NlaIII CATG 2 cut(s) 193, 499
NmeAIII GCCGAG 1 cut(s) 302
NmuCI GTSAC 1 cut(s) 196
NsiI ATGCAT 1 cut(s) 12
PagI TCATGA 1 cut(s) 189
PceI AGGCCT 1 cut(s) 280
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 329
PctI GAATGC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 1 cut(s) 226
PsuI RGATCY 1 cut(s) 483
RseI CAYNNNNRTG 1 cut(s) 194
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 4 cut(s) 147, 283, 447, 483
SduI GDGCHC 1 cut(s) 438
SetI ASST 9 cut(s) 67, 159, 198, 214, 221, 307, 340, 347, 361
SfaNI GCATC 1 cut(s) 19
SfcI CTRYAG 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 1 cut(s) 36
SseBI AGGCCT 1 cut(s) 280
SsiI CCGC 2 cut(s) 171, 224
StuI AGGCCT 1 cut(s) 280
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 2 cut(s) 202, 368
TaiI ACGT 1 cut(s) 198
TaqI TCGA 3 cut(s) 23, 72, 384
TasI AATT 1 cut(s) 36
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 102
TseFI GTSAC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 5 cut(s) 17, 90, 206, 232, 516
TspGWI ACGGA 3 cut(s) 43, 114, 444
XmnI GAANNNNTTC 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.