AT1G09700

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004669
Physical Location & Seq
Reverse (-)
3137649 .. 3140353
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09700.2

Sequence Viewer

Length: 1260 bp
ATGACCTCCACTGATGTTTCCTCTGGTGTTTCCAATTGCTATGTTTTCAAAAGTCGGTTGCAGGAGTATGCTCAGAAGTACAAGCTCCCAACGCCTGTTTATGAGATCGTTAAAGAAGGCCCTTCACACAAATCTTTATTTCAATCGACTGTGATACTGGATGGTGTCAGATATAATTCTTTGCCTGGATTCTTCAATCGTAAGGCTGCAGAGCAATCAGCTGCCGAGGTTGCTCTCCGGGAATTAGCAAAATCCAGTGAGCTAAGCCAATGTGTTTCACAACCTGTTCACGAAACGGGATTATGCAAGAACCTACTTCAAGAATACGCTCAAAAGATGAATTACGCGATTCCATTGTATCAGTGCCAGAAGGTCGAAACTCTTGGGAGAGTTACACAATTCACATGTACTGTAGAGATTGGAGGCATAAAGTACACAGGAGCTGCAACAAGAACTAAAAAAGATGCTGAGATTAGCGCTGGGAGAACTGCTCTTTTAGCGATCCAGTCAGACACTAAAAACAACCTTGCCAACTACAACACTCAGCTTACTGTACTTCCTTGTGAGAAGAAGACAATACAGGCAGCTATCCCGCTTAAAGAAACCGTCAAGACTCTTAAAGCCAGGAAGGCTCAGTTCAAGAAGAAAGCTCAGAAAGGAAAACGCACAGTAGCTAAAAATCCTGAAGATATTATCATCCCTCCTCAACCTACTGATCATTGTCAAAACGATCAGTCAGAGAAAATTGAGACAACGCCAAATCTGGAACCTTCTTCATGCATGAACGGGTTAAAGGAGGCAGCATTTGGGAGTGTGGAGACAGAGAAAATTGAGACAACGCCAAATCTGGAACCTCCTTCATGCATGAACGGGTTAAAGGAGGCAGCATTTGGGAGTGTGGAGACAGAGAAAATTGAGACAACGCCAAATCTGGAACCTCCTTCATGCATGAACGGGTTAAAGGAGGCAGCATTTGGGAGTGTGGAGACAGAGAAAATTGAGACAACGCCAAATCTGGAACCTTCTTCATGCATGAACGGGTTAAAGGAGGCAGCATTTGGGAGTGTGGAGACAGAGAAAATTGAGACAACGCCAAATCTGGAACCTCCTTCATGCATGAACGGGTTAAAGGAGGCAGCATTTGGGAGTGTGGAGACAGAGAAAATTGAGACAACGCCAAATCTGGAATCTTCTTCATGCATGAGCGGGTTAAAGGAGGCAGCATTTGGGAGTGTGGAGACAGAAGCAAGCCACGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009892 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010267 GO:0010305 GO:0010445 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031053 GO:0031054 GO:0031974 GO:0031981 GO:0032296 GO:0032501 GO:0032502 GO:0033993 GO:0034470 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0035198 GO:0035279 GO:0040029 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097305 GO:0098795 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

419

Amino Acids

45.55

Weight (kDa)

5.6

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 17 - 82 4.1e-17 Double-stranded RNA binding motif
dsrm PF00035 103 - 166 2.9e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1206
AccII CGCG 1 cut(s) 347
AciI CCGC 2 cut(s) 593, 1206
AclWI GGATC 1 cut(s) 496
AcuI CTGAAG 1 cut(s) 705
AfaI GTAC 4 cut(s) 80, 409, 434, 555
AfeI AGCGCT 1 cut(s) 478
AflIII ACRYGT 1 cut(s) 404
AgsI TTSAA 5 cut(s) 49, 143, 196, 320, 640
AjnI CCWGG 2 cut(s) 184, 623
AluBI AGCT 8 cut(s) 85, 221, 262, 443, 547, 587, 650, 674
AluI AGCT 8 cut(s) 85, 221, 262, 443, 547, 587, 650, 674
AlwI GGATC 1 cut(s) 496
AlwNI CAGNNNCTG 1 cut(s) 443
Aor51HI AGCGCT 1 cut(s) 478
AoxI GGCC 1 cut(s) 118
AspLEI GCGC 1 cut(s) 479
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 578
BccI CCATC 1 cut(s) 155
BciT130I CCWGG 2 cut(s) 186, 625
BclI TGATCA 1 cut(s) 715
BcnI CCSGG 1 cut(s) 239
BfmI CTRYAG 2 cut(s) 207, 411
BfoI RGCGCY 1 cut(s) 480
BglI GCCNNNNNGGC 1 cut(s) 629
BlpI GCTNAGC 1 cut(s) 263
Bme1390I CCNGG 3 cut(s) 186, 239, 625
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 5 cut(s) 768, 852, 936, 1020, 1104
BmrFI CCNGG 3 cut(s) 186, 239, 625
BmsI GCATC 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 578
BplI GAGNNNNNCTC 2 cut(s) 475, 507
Bpu1102I GCTNAGC 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 225
Bse1I ACTGG 3 cut(s) 162, 255, 505
BseBI CCWGG 2 cut(s) 186, 625
BseDI CCNNGG 1 cut(s) 225
BseGI GGATG 2 cut(s) 166, 696
BseMII CTCAG 5 cut(s) 86, 459, 557, 647, 665
BseNI ACTGG 3 cut(s) 162, 255, 505
BseRI GAGGAG 1 cut(s) 693
BseYI CCCAGC 1 cut(s) 479
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 1 cut(s) 120
BsiSI CCGG 1 cut(s) 238
BsnI GGCC 1 cut(s) 120
Bsp143I GATC 4 cut(s) 105, 501, 715, 730
Bsp1720I GCTNAGC 1 cut(s) 263
BspACI CCGC 2 cut(s) 593, 1206
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 5 cut(s) 85, 460, 556, 646, 664
BspFNI CGCG 1 cut(s) 347
BspLI GGNNCC 5 cut(s) 768, 852, 936, 1020, 1104
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 1 cut(s) 496
BsrBI CCGCTC 1 cut(s) 1206
BsrI ACTGG 3 cut(s) 162, 255, 505
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 4 cut(s) 105, 501, 715, 730
Bst2UI CCWGG 2 cut(s) 186, 625
Bst4CI ACNGT 5 cut(s) 151, 412, 553, 607, 670
BstC8I GCNNGC 1 cut(s) 1249
BstDEI CTNAG 6 cut(s) 72, 263, 468, 543, 633, 651
BstF5I GGATG 2 cut(s) 166, 696
BstFNI CGCG 1 cut(s) 347
BstH2I RGCGCY 1 cut(s) 480
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 4 cut(s) 108, 504, 718, 733
BstMBI GATC 4 cut(s) 105, 501, 715, 730
BstMWI GCNNNNNNNGC 5 cut(s) 91, 230, 497, 629, 1253
BstNI CCWGG 2 cut(s) 186, 625
BstNSI RCATGY 1 cut(s) 408
BstSCI CCNGG 3 cut(s) 184, 237, 623
BstSFI CTRYAG 2 cut(s) 207, 411
BstUI CGCG 1 cut(s) 347
BstV2I GAAGAC 1 cut(s) 578
BsuRI GGCC 1 cut(s) 120
BtsCI GGATG 2 cut(s) 166, 696
BtsIMutI CAGTG 3 cut(s) 9, 262, 368
Cac8I GCNNGC 1 cut(s) 1249
CaiI CAGNNNCTG 1 cut(s) 443
CfoI GCGC 1 cut(s) 479
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 4 cut(s) 79, 408, 433, 554
CviQI GTAC 4 cut(s) 79, 408, 433, 554
DdeI CTNAG 6 cut(s) 72, 263, 468, 543, 633, 651
DpnI GATC 4 cut(s) 107, 503, 717, 732
DpnII GATC 4 cut(s) 105, 501, 715, 730
Eco47III AGCGCT 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 705
EcoO109I RGGNCCY 1 cut(s) 119
EcoRII CCWGG 2 cut(s) 184, 623
EcoT22I ATGCAT 6 cut(s) 782, 866, 950, 1034, 1118, 1202
FauI CCCGC 2 cut(s) 600, 1199
FbaI TGATCA 1 cut(s) 715
FokI GGATG 2 cut(s) 173, 683
GlaI GCGC 1 cut(s) 478
GsaI CCCAGC 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 480
HaeIII GGCC 1 cut(s) 120
HapII CCGG 1 cut(s) 238
HhaI GCGC 1 cut(s) 479
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HinfI GANTC 4 cut(s) 189, 349, 613, 1187
HpaII CCGG 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 289, 435
Hpy188I TCNGA 5 cut(s) 75, 170, 511, 654, 739
Hpy8I GTNNAC 2 cut(s) 289, 435
HpyAV CCTTC 9 cut(s) 110, 132, 364, 622, 780, 867, 951, 1032, 1119
HpyCH4III ACNGT 5 cut(s) 151, 412, 553, 607, 670
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 230, 497, 629, 1253
HpyF3I CTNAG 6 cut(s) 72, 263, 468, 543, 633, 651
HspAI GCGC 1 cut(s) 477
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 4 cut(s) 105, 501, 715, 730
LmnI GCTCC 2 cut(s) 90, 440
LweI GCATC 1 cut(s) 454
MaeIII GTNAC 1 cut(s) 391
MalI GATC 4 cut(s) 107, 503, 717, 732
MbiI CCGCTC 1 cut(s) 1206
MboI GATC 4 cut(s) 105, 501, 715, 730
MboII GAAGA 9 cut(s) 184, 580, 583, 655, 698, 765, 1017, 1182, 1185
MfeI CAATTG 1 cut(s) 34
MlyI GAGTC 1 cut(s) 607
Mph1103I ATGCAT 6 cut(s) 782, 866, 950, 1034, 1118, 1202
MseI TTAA 9 cut(s) 111, 597, 618, 791, 875, 959, 1043, 1127, 1211
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 238
MspR9I CCNGG 3 cut(s) 186, 239, 625
MunI CAATTG 1 cut(s) 34
MvaI CCWGG 2 cut(s) 186, 625
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 5 cut(s) 91, 230, 497, 629, 1253
NciI CCSGG 1 cut(s) 239
NdeII GATC 4 cut(s) 105, 501, 715, 730
NlaIV GGNNCC 5 cut(s) 768, 852, 936, 1020, 1104
NmeAIII GCCGAG 1 cut(s) 250
NsiI ATGCAT 6 cut(s) 782, 866, 950, 1034, 1118, 1202
NspI RCATGY 1 cut(s) 408
PciI ACATGT 1 cut(s) 404
PfeI GAWTC 3 cut(s) 189, 349, 1187
PfoI TCCNGGA 1 cut(s) 237
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
PscI ACATGT 1 cut(s) 404
Psp6I CCWGG 2 cut(s) 184, 623
PspFI CCCAGC 1 cut(s) 479
PspGI CCWGG 2 cut(s) 184, 623
PspN4I GGNNCC 5 cut(s) 768, 852, 936, 1020, 1104
PspPI GGNCC 1 cut(s) 119
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 443
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 4 cut(s) 80, 409, 434, 555
RsaNI GTAC 4 cut(s) 79, 408, 433, 554
SaqAI TTAA 9 cut(s) 111, 597, 618, 791, 875, 959, 1043, 1127, 1211
Sau3AI GATC 4 cut(s) 105, 501, 715, 730
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 1 cut(s) 607
ScrFI CCNGG 3 cut(s) 186, 239, 625
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 2 cut(s) 207, 411
SsiI CCGC 2 cut(s) 593, 1206
StyD4I CCNGG 3 cut(s) 184, 237, 623
TaaI ACNGT 5 cut(s) 151, 412, 553, 607, 670
TaqI TCGA 2 cut(s) 146, 375
TatI WGTACW 4 cut(s) 78, 407, 432, 553
TfiI GAWTC 3 cut(s) 189, 349, 1187
Tru1I TTAA 9 cut(s) 111, 597, 618, 791, 875, 959, 1043, 1127, 1211
Tru9I TTAA 9 cut(s) 111, 597, 618, 791, 875, 959, 1043, 1127, 1211
TscAI CASTG 3 cut(s) 16, 262, 368
TspRI CASTG 3 cut(s) 16, 262, 368
XceI RCATGY 1 cut(s) 408
Zsp2I ATGCAT 6 cut(s) 782, 866, 950, 1034, 1118, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.