FvH4_5g35180

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004669
Physical Location & Seq
Forward (+)
25748728 .. 25753310
4583 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35180.t6

Sequence Viewer

Length: 1326 bp
ATGGCTAAGAGTGAAGAATTTCAAGGTGTTTCAAAGTGCTATGTATTCAAGAGCCGGTTGCAAGAGTATGCACAGAAAATGGGGATAAAGATACCAGTATATGAGACTACAAAAGAAGGTCCTTCACATGAGCCATCCTTCAGGTCTACTGTGGTAGTAAATGATGTTAGATACGACTCATTGGTTGGGTTTACTCATCGTAAGGCAGCTGAACAGTCAGCTGCCGAAGTTGCTCTGCGGGAGTTATCTAAATCTGGCGACGCTGCCAAATGCATCTCTCAACCTGTTCAAGAAATTGGCTTATGCAAGAATCTGCTTCAAGAGTATGCACAAAAGATGAATTTTTCTGTTCCTGTATATCAGTGTAAGACGGATTTTTCCTCTCGCAAAACACCAGTATATTCATGTACCGTTGAGATTGGGGGAATTCGTTATATTGGAGCTACAGCAACAACCAAAAAGGAAGCTGAGATAAAAGTGGCTAGAACTGCTCTCTTAGCTATCTGGTTAAGTAGTAATGAGTCATCCATGGGATCATTTGAAACCTCTGAGTTAACAGTGCTTCCATCTAAGAAAAGGCGGTTAGAATCTGAAGAGGCTTCTAATAAACCTAAACGAAAAAAAGTTCCATTTAGTAGGAAGAGAATGCTCAAACGGAAACCTAATGAAGAAAATGCAGTACCAGGATTAGAGAGTAATGAAGCTATGCCACAAGACTCAGTGTCTGAGGATATTCCCCATGCTAATAATGTTTATGCTGAGCATGGGCCATCAACAGCTTCAGATCTCACCAACGGGAGCAATGGGAATGGGTTTGTGGGCTGTGGGGACATAACTACTTTGGCCAAGGAAGTGAATATGGTGTCTGCTGTTAAGCCAATGACTGCAGAAGCTATGCCACAGGACTCAGTGTCCGAGGATATTCCCCATGCTAATAATGTTTATGCTGAGCATGGGCCATCAACAGCTTCAGATCTCACCAACGGGAGCAATGGGAATGGGTTTGTGGGCTGTGGGGACTTAACTACTTTGGCCAAGGAAGTGAATATGGTGTCTGCTGTTAAACCAATGACCGCAGAAGCTATGCCACAGGACTCAGTGTTTGAGCATATTCCCCATGCTAACAATGTCAATGCTGAACACGGGCTGTCAACTACTTCAGATTTCAACGCCACCAATGGGAGTTGCGCTGTAGGGGATTCAATTTCACCGTCTTCTGAAAACACAACTACTTTGGCCAAGGAAGGGAATATGGTGTCTGCTGGACCTTATTTGGAGGCTTCAAGTGTCATGCCTGGACCTGAGTCACTGGACCAATCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009892 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010267 GO:0010305 GO:0010445 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031053 GO:0031054 GO:0031974 GO:0031981 GO:0032296 GO:0032501 GO:0032502 GO:0033993 GO:0034470 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0035198 GO:0035279 GO:0040029 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097305 GO:0098795 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

442

Amino Acids

47.38

Weight (kDa)

5.53

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 17 - 82 5e-17 Double-stranded RNA binding motif
dsrm PF00035 103 - 166 4e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 146
AciI CCGC 3 cut(s) 238, 580, 1074
AclWI GGATC 1 cut(s) 541
AcoI YGGCCR 3 cut(s) 843, 1032, 1236
AcsI RAATTY 3 cut(s) 17, 340, 426
AcuI CTGAAG 5 cut(s) 124, 612, 765, 954, 1143
AfaI GTAC 2 cut(s) 409, 681
AfiI CCNNNNNNNGG 2 cut(s) 1179, 1245
AgsI TTSAA 9 cut(s) 23, 33, 49, 290, 320, 542, 1168, 1203, 1284
AhdI GACNNNNNGTC 2 cut(s) 721, 910
AjnI CCWGG 2 cut(s) 682, 1294
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 1 cut(s) 541
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 5 cut(s) 767, 843, 956, 1032, 1236
ApeKI GCWGC 3 cut(s) 206, 221, 263
ApoI RAATTY 3 cut(s) 17, 340, 426
ArsI GACNNNNNNTTYG 2 cut(s) 1085, 1117
Asp700I GAANNNNTTC 1 cut(s) 18
AspLEI GCGC 1 cut(s) 1190
AspS9I GGNCC 6 cut(s) 119, 767, 956, 1265, 1298, 1312
AsuHPI GGTGA 3 cut(s) 781, 970, 1200
AvaII GGWCC 4 cut(s) 119, 1265, 1298, 1312
BalI TGGCCA 3 cut(s) 845, 1034, 1238
BbsI GAAGAC 1 cut(s) 1206
BbvI GCAGC 3 cut(s) 208, 218, 250
BccI CCATC 4 cut(s) 142, 574, 778, 967
BciT130I CCWGG 2 cut(s) 684, 1296
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 3 cut(s) 444, 885, 1191
BglII AGATCT 2 cut(s) 784, 973
BisI GCNGC 3 cut(s) 207, 222, 264
BlpI GCTNAGC 2 cut(s) 759, 948
BlsI GCNGC 3 cut(s) 208, 223, 265
Bme1390I CCNGG 2 cut(s) 684, 1296
Bme18I GGWCC 4 cut(s) 119, 1265, 1298, 1312
BmeRI GACNNNNNGTC 2 cut(s) 721, 910
BmgT120I GGNCC 6 cut(s) 119, 767, 956, 1265, 1298, 1312
BmrFI CCNGG 2 cut(s) 684, 1296
BmsI GCATC 1 cut(s) 282
BoxI GACNNNNGTC 1 cut(s) 1303
BpiI GAAGAC 1 cut(s) 1206
Bpu1102I GCTNAGC 2 cut(s) 759, 948
BsaJI CCNNGG 5 cut(s) 528, 846, 915, 1035, 1239
Bsc4I CCNNNNNNNGG 2 cut(s) 1179, 1245
Bse118I RCCGGY 1 cut(s) 54
Bse1I ACTGG 3 cut(s) 95, 395, 1314
Bse3DI GCAATG 2 cut(s) 808, 997
BseBI CCWGG 2 cut(s) 684, 1296
BseDI CCNNGG 5 cut(s) 528, 846, 915, 1035, 1239
BseGI GGATG 2 cut(s) 134, 524
BseLI CCNNNNNNNGG 2 cut(s) 1179, 1245
BseMI GCAATG 2 cut(s) 808, 997
BseMII CTCAG 9 cut(s) 459, 540, 717, 732, 750, 921, 939, 1110, 1293
BseNI ACTGG 3 cut(s) 95, 395, 1314
BseXI GCAGC 3 cut(s) 208, 218, 250
BshFI GGCC 5 cut(s) 769, 845, 958, 1034, 1238
BsiSI CCGG 1 cut(s) 55
BslFI GGGAC 2 cut(s) 842, 1031
BslI CCNNNNNNNGG 2 cut(s) 1179, 1245
BsmAI GTCTC 1 cut(s) 98
BsmFI GGGAC 2 cut(s) 842, 1031
BsmI GAATGC 1 cut(s) 651
BsnI GGCC 5 cut(s) 769, 845, 958, 1034, 1238
Bsp143I GATC 3 cut(s) 533, 784, 973
Bsp1720I GCTNAGC 2 cut(s) 759, 948
Bsp19I CCATGG 1 cut(s) 528
BspACI CCGC 3 cut(s) 238, 580, 1074
BspANI GGCC 5 cut(s) 769, 845, 958, 1034, 1238
BspCNI CTCAG 9 cut(s) 460, 541, 718, 731, 751, 920, 940, 1109, 1294
BspMAI CTGCAG 1 cut(s) 889
BspPI GGATC 1 cut(s) 541
BsrDI GCAATG 2 cut(s) 808, 997
BsrFI RCCGGY 1 cut(s) 54
BsrI ACTGG 3 cut(s) 95, 395, 1314
BssAI RCCGGY 1 cut(s) 54
BssECI CCNNGG 5 cut(s) 528, 846, 915, 1035, 1239
BssMI GATC 3 cut(s) 533, 784, 973
BssT1I CCWWGG 4 cut(s) 528, 846, 1035, 1239
Bst2UI CCWGG 2 cut(s) 684, 1296
Bst4CI ACNGT 6 cut(s) 151, 216, 412, 559, 1212, 1323
Bst6I CTCTTC 2 cut(s) 588, 635
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 2 cut(s) 134, 524
BstHHI GCGC 1 cut(s) 1190
BstKTI GATC 3 cut(s) 536, 787, 976
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 3 cut(s) 533, 784, 973
BstMWI GCNNNNNNNGC 3 cut(s) 230, 488, 497
BstNI CCWGG 2 cut(s) 684, 1296
BstPAI GACNNNNGTC 1 cut(s) 1303
BstSCI CCNGG 2 cut(s) 682, 1294
BstSFI CTRYAG 3 cut(s) 444, 885, 1191
BstV1I GCAGC 3 cut(s) 208, 218, 250
BstV2I GAAGAC 1 cut(s) 1206
BstX2I RGATCY 2 cut(s) 784, 973
BstYI RGATCY 2 cut(s) 784, 973
BsuRI GGCC 5 cut(s) 769, 845, 958, 1034, 1238
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 2 cut(s) 134, 524
BtsIMutI CAGTG 6 cut(s) 368, 564, 726, 915, 1104, 1307
CaiI CAGNNNCTG 1 cut(s) 725
CfoI GCGC 1 cut(s) 1190
Cfr10I RCCGGY 1 cut(s) 54
Cfr13I GGNCC 6 cut(s) 119, 767, 956, 1265, 1298, 1312
CseI GACGC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 408, 680
CviAII CATG 9 cut(s) 128, 405, 529, 740, 764, 929, 953, 1118, 1291
CviQI GTAC 2 cut(s) 408, 680
DpnI GATC 3 cut(s) 535, 786, 975
DpnII GATC 3 cut(s) 533, 784, 973
DriI GACNNNNNGTC 2 cut(s) 721, 910
EaeI YGGCCR 3 cut(s) 843, 1032, 1236
Eam1104I CTCTTC 2 cut(s) 588, 635
Eam1105I GACNNNNNGTC 2 cut(s) 721, 910
EarI CTCTTC 2 cut(s) 588, 635
Eco130I CCWWGG 4 cut(s) 528, 846, 1035, 1239
Eco47I GGWCC 4 cut(s) 119, 1265, 1298, 1312
Eco57I CTGAAG 5 cut(s) 124, 612, 765, 954, 1143
EcoO109I RGGNCCY 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 2 cut(s) 682, 1294
EcoT14I CCWWGG 4 cut(s) 528, 846, 1035, 1239
EcoT22I ATGCAT 1 cut(s) 275
ErhI CCWWGG 4 cut(s) 528, 846, 1035, 1239
FaeI CATG 9 cut(s) 131, 408, 532, 743, 767, 932, 956, 1121, 1294
FaqI GGGAC 2 cut(s) 842, 1031
FatI CATG 9 cut(s) 127, 404, 528, 739, 763, 928, 952, 1117, 1290
FauI CCCGC 1 cut(s) 231
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 3 cut(s) 207, 222, 264
FokI GGATG 2 cut(s) 121, 511
Fsp4HI GCNGC 3 cut(s) 207, 222, 264
FspBI CTAG 1 cut(s) 483
GlaI GCGC 1 cut(s) 1189
GluI GCNGC 3 cut(s) 207, 222, 264
HaeIII GGCC 5 cut(s) 769, 845, 958, 1034, 1238
HapII CCGG 1 cut(s) 55
HgaI GACGC 1 cut(s) 269
HhaI GCGC 1 cut(s) 1190
Hin1II CATG 9 cut(s) 131, 408, 532, 743, 767, 932, 956, 1121, 1294
Hin6I GCGC 1 cut(s) 1188
HinP1I GCGC 1 cut(s) 1188
HincII GTYRAC 2 cut(s) 555, 1152
HindII GTYRAC 2 cut(s) 555, 1152
HinfI GANTC 9 cut(s) 176, 310, 521, 587, 716, 905, 1094, 1199, 1304
HpaI GTTAAC 1 cut(s) 555
HpaII CCGG 1 cut(s) 55
HphI GGTGA 3 cut(s) 781, 970, 1200
Hpy166II GTNNAC 4 cut(s) 147, 192, 555, 1152
Hpy188I TCNGA 8 cut(s) 550, 592, 727, 784, 916, 973, 1162, 1219
Hpy188III TCNNGA 3 cut(s) 49, 290, 320
Hpy8I GTNNAC 4 cut(s) 147, 192, 555, 1152
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 4 cut(s) 110, 132, 148, 1238
HpyCH4III ACNGT 6 cut(s) 151, 216, 412, 559, 1212, 1323
HpyCH4V TGCA 7 cut(s) 61, 71, 273, 306, 329, 677, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 488, 497
Hsp92II CATG 9 cut(s) 131, 408, 532, 743, 767, 932, 956, 1121, 1294
HspAI GCGC 1 cut(s) 1188
KspAI GTTAAC 1 cut(s) 555
Kzo9I GATC 3 cut(s) 533, 784, 973
LmnI GCTCC 3 cut(s) 440, 798, 987
Lsp1109I GCAGC 3 cut(s) 208, 218, 250
LweI GCATC 1 cut(s) 282
MaeI CTAG 1 cut(s) 483
MaeIII GTNAC 1 cut(s) 1305
MalI GATC 3 cut(s) 535, 786, 975
MboI GATC 3 cut(s) 533, 784, 973
MboII GAAGA 5 cut(s) 26, 605, 652, 680, 1206
MflI RGATCY 2 cut(s) 784, 973
MlsI TGGCCA 3 cut(s) 845, 1034, 1238
MluCI AATT 5 cut(s) 17, 294, 340, 426, 1203
MluNI TGGCCA 3 cut(s) 845, 1034, 1238
MlyI GAGTC 6 cut(s) 170, 530, 710, 899, 1088, 1313
MnlI CCTC 6 cut(s) 391, 556, 589, 721, 910, 1270
Mox20I TGGCCA 3 cut(s) 845, 1034, 1238
Mph1103I ATGCAT 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 18
MscI TGGCCA 3 cut(s) 845, 1034, 1238
MseI TTAA 5 cut(s) 509, 554, 873, 1022, 1062
Msp20I TGGCCA 3 cut(s) 845, 1034, 1238
MspA1I CMGCKG 2 cut(s) 209, 221
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 2 cut(s) 684, 1296
Mva1269I GAATGC 1 cut(s) 651
MvaI CCWGG 2 cut(s) 684, 1296
MwoI GCNNNNNNNGC 3 cut(s) 230, 488, 497
NcoI CCATGG 1 cut(s) 528
NdeII GATC 3 cut(s) 533, 784, 973
NlaIII CATG 9 cut(s) 131, 408, 532, 743, 767, 932, 956, 1121, 1294
NmuCI GTSAC 1 cut(s) 1305
NsiI ATGCAT 1 cut(s) 275
PctI GAATGC 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 3 cut(s) 310, 587, 1199
PkrI GCNGC 3 cut(s) 208, 223, 265
PleI GAGTC 6 cut(s) 170, 529, 710, 899, 1088, 1312
PpsI GAGTC 6 cut(s) 170, 529, 710, 899, 1088, 1312
PpuMI RGGWCCY 1 cut(s) 119
PshAI GACNNNNGTC 1 cut(s) 1303
Psp5II RGGWCCY 1 cut(s) 119
Psp6I CCWGG 2 cut(s) 682, 1294
PspGI CCWGG 2 cut(s) 682, 1294
PspPI GGNCC 6 cut(s) 119, 767, 956, 1265, 1298, 1312
PspPPI RGGWCCY 1 cut(s) 119
PstI CTGCAG 1 cut(s) 889
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 2 cut(s) 784, 973
PvuII CAGCTG 2 cut(s) 209, 221
RsaI GTAC 2 cut(s) 409, 681
RsaNI GTAC 2 cut(s) 408, 680
SaqAI TTAA 5 cut(s) 509, 554, 873, 1022, 1062
SatI GCNGC 3 cut(s) 207, 222, 264
Sau3AI GATC 3 cut(s) 533, 784, 973
Sau96I GGNCC 6 cut(s) 119, 767, 956, 1265, 1298, 1312
SchI GAGTC 6 cut(s) 170, 530, 710, 899, 1088, 1313
ScrFI CCNGG 2 cut(s) 684, 1296
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 3 cut(s) 444, 885, 1191
SinI GGWCC 4 cut(s) 119, 1265, 1298, 1312
Sse9I AATT 5 cut(s) 17, 294, 340, 426, 1203
SsiI CCGC 3 cut(s) 238, 580, 1074
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 2 cut(s) 682, 1294
StyI CCWWGG 4 cut(s) 528, 846, 1035, 1239
TaaI ACNGT 6 cut(s) 151, 216, 412, 559, 1212, 1323
TasI AATT 5 cut(s) 17, 294, 340, 426, 1203
TfiI GAWTC 3 cut(s) 310, 587, 1199
Tru1I TTAA 5 cut(s) 509, 554, 873, 1022, 1062
Tru9I TTAA 5 cut(s) 509, 554, 873, 1022, 1062
TscAI CASTG 6 cut(s) 368, 564, 726, 915, 1104, 1314
TseFI GTSAC 1 cut(s) 1305
TseI GCWGC 3 cut(s) 206, 221, 263
Tsp45I GTSAC 1 cut(s) 1305
TspDTI ATGAA 4 cut(s) 353, 393, 681, 714
TspGWI ACGGA 2 cut(s) 386, 670
TspRI CASTG 6 cut(s) 368, 564, 726, 915, 1104, 1314
VpaK11BI GGWCC 4 cut(s) 119, 1265, 1298, 1312
XapI RAATTY 3 cut(s) 17, 340, 426
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 1 cut(s) 483
Zsp2I ATGCAT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.