Rroxscaffold_3G00225270

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004669
Physical Location & Seq
Reverse (-)
8461341 .. 8465758
4418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225270.1

Sequence Viewer

Length: 1785 bp
ATGGCTACCAATGCAGAATTTAAAGGTGTTTCAAAATGCTATGTATTCAAGAGCCGGTTGCAAGAGTATGCACAGAAAATGGGAATAAAGACACCAGTATATCAGACTACAAAAGAAGGTCCTTCACATGAGCCTTCCTTCAGGTCTACTGTGATATTAAATGATGTTAGATACGACTCGTTGCTTGGGTTTACTAATCGTAAGGCAGCTGAACAGTCAGCTGCTGAAGTTGCTCTGGTGGAGTTATCTAAATCTGGTGATGCTGCACAATGCATCTCTCAACCTGTTCATGAAATTGGCTTATGCAAAAATCTGCTTCAAGAGTATGCACAGAAGATGAATTATGCTGTTCCTGTATATCAGTGTCAGAGGGATATTTCTTCTGGCAAAGTACCACTGTATTCATGTACTGTCGAGATTGGGGGAATTCGTTATATTGGAGCTGCAGCAACAACCAAAAAGGAATCTGAGATAAAAGTGGCTAGAACTGCTCTTCTAGCTCTCTTGTTAAGTGATACTGAGTCATCCATGGGATCAGTTGGCAGCTCTGAGTTAACAGTGCTTCCATCTAAAAGAAAGCGGTCAGAATGTGAAGAGATTTCTAATGAACCTAAACCAAAGAAACCCCCATATAAGAGGAGAATGTTCAAAAAGAAAGCCTATGAAGAAAAGGTGGGCCAGACTGAAGTTGGAAATATAGCACCAGGATCAGAGAGTAATGAATCCATTAAGCCAATGACTGCAGAAGCTATGCCACAAGAGCCAGTGTCCGAAGATATTCCCCATGCTAATAATGTCAATGCTGAACATGGGCCATCAATAGCTTCAGATTTCACCAACAGCAGCATTGTGAGTGGGAATGTGGGTTCAGTTTCACTGTCCTGTGGAAACATAACTACTTTGGCCAAGGAAGTGGATATGGTGTCTGCCGTTAAGCCAATGACTGCGGAAGCTTTGCCACAAGACTCAGTGTCCGAGTATATTACCCGTGCTAATAATCTCAACGCTCAACATGGGCTGACAACTGCTTCAGATTTCACCAACAGCAGCAATGGAAGTGGGGATGTGGGTTCAGTTTCACTGTCCTGTGGAAACACAACTGCTTCGGGTAAGGAAGTGAATATGGTGTCTTCTGTTAAGCCAATGACTGCAGGAACTATGCCACAAGATTCAGTGTCTGAGGATATTCCCCATGCTAATATTGTCAATGCTGAACATGGGCTGTCAACAGCTTCAGATTTCACCAACAGCAGCAATGGGAGTGGAGATGTGGGTTCAGTTTCACTGCCCTCTGGAAACATAACTACTTTGGCCAAGGAAGCGGATATGGTGTCTGCCGTTAAGCCAATGACTGCAGAAGCTATGCCACAAGACTCAGTGTCTGAGGATATTCCCCATGCTAATAATGTTAATGCTGAGCCGTTAACAGCTTCAGATTTCACCAACGGCAGCAATGGGAGTTGGGATGTGGGTTCAATGTCACTGTCCTGCGGAAACACAACTACTTCGGCCAAGGAAGTGAATATGGTTGCTGCTGTTAAGCCAGTGTCTGCAGAAATGATGCCACAAGATTCGGTGTCTGAGGATATTCCCCATGCTAATAATGTGAATGCTGAACATGGGCCGTCAGCAGCTTCAGATTTCACCAACGGCAGCAATGTGAGTGGAGATGTGGGTTCAGTTTCACTGTCCTCTGGAGACACAACTGCTTCGGCCAAGGAAGTGAATATGGTGTCTTCTGGACCTTGTTTGGAGGCTTCAAGCGTCATGCCTGGACCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003002 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009892 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010267 GO:0010305 GO:0010445 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031053 GO:0031054 GO:0031974 GO:0031981 GO:0032296 GO:0032501 GO:0032502 GO:0033993 GO:0034470 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0035198 GO:0035279 GO:0040029 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0097305 GO:0098795 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

594

Amino Acids

62.17

Weight (kDa)

4.94

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 17 - 79 6.3e-16 Double-stranded RNA binding motif
dsrm PF00035 103 - 167 5e-12 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 146
AciI CCGC 4 cut(s) 580, 947, 1322, 1491
AclWI GGATC 2 cut(s) 541, 715
AcoI YGGCCR 4 cut(s) 903, 1311, 1509, 1713
AcsI RAATTY 2 cut(s) 17, 426
AcuI CTGAAG 8 cut(s) 124, 246, 705, 810, 1014, 1218, 1416, 1620
AfaI GTAC 2 cut(s) 393, 409
AgsI TTSAA 6 cut(s) 33, 49, 320, 649, 1476, 1761
AhdI GACNNNNNGTC 2 cut(s) 970, 1378
AjnI CCWGG 2 cut(s) 703, 1771
AloI GAACNNNNNNTCC 2 cut(s) 850, 882
Alw26I GTCTC 1 cut(s) 1692
AlwI GGATC 2 cut(s) 541, 715
AlwNI CAGNNNCTG 4 cut(s) 224, 1178, 1382, 1550
AoxI GGCC 7 cut(s) 676, 812, 903, 1311, 1509, 1622, 1713
ApoI RAATTY 2 cut(s) 17, 426
Asp700I GAANNNNTTC 1 cut(s) 777
AspS9I GGNCC 6 cut(s) 119, 676, 812, 1622, 1742, 1775
AsuHPI GGTGA 6 cut(s) 269, 826, 1030, 1234, 1432, 1636
AvaII GGWCC 3 cut(s) 119, 1742, 1775
BalI TGGCCA 2 cut(s) 905, 1313
BbsI GAAGAC 2 cut(s) 1122, 1728
BccI CCATC 2 cut(s) 574, 823
BceAI ACGGC 6 cut(s) 914, 1322, 1405, 1462, 1609, 1666
BciT130I CCWGG 2 cut(s) 705, 1773
BcoDI GTCTC 1 cut(s) 1692
BfaI CTAG 2 cut(s) 483, 497
BfmI CTRYAG 5 cut(s) 444, 741, 1149, 1353, 1551
BlpI GCTNAGC 1 cut(s) 1416
Bme1390I CCNGG 2 cut(s) 705, 1773
Bme18I GGWCC 3 cut(s) 119, 1742, 1775
BmeRI GACNNNNNGTC 2 cut(s) 970, 1378
BmgT120I GGNCC 6 cut(s) 119, 676, 812, 1622, 1742, 1775
BmrFI CCNGG 2 cut(s) 705, 1773
BmsI GCATC 3 cut(s) 250, 282, 1551
BpiI GAAGAC 2 cut(s) 1122, 1728
BpmI CTGGAG 1 cut(s) 1716
Bpu1102I GCTNAGC 1 cut(s) 1416
BsaJI CCNNGG 5 cut(s) 528, 906, 1314, 1512, 1716
Bse118I RCCGGY 1 cut(s) 54
Bse1I ACTGG 3 cut(s) 95, 764, 1544
Bse3DI GCAATG 4 cut(s) 1057, 1261, 1459, 1663
BseBI CCWGG 2 cut(s) 705, 1773
BseDI CCNNGG 5 cut(s) 528, 906, 1314, 1512, 1716
BseGI GGATG 3 cut(s) 524, 1069, 1471
BseMI GCAATG 4 cut(s) 1057, 1261, 1459, 1663
BseMII CTCAG 9 cut(s) 459, 510, 540, 981, 1170, 1374, 1389, 1407, 1572
BseNI ACTGG 3 cut(s) 95, 764, 1544
BseRI GAGGAG 1 cut(s) 652
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 7 cut(s) 678, 814, 905, 1313, 1511, 1624, 1715
BsiSI CCGG 1 cut(s) 55
BsmAI GTCTC 1 cut(s) 1692
BsmI GAATGC 1 cut(s) 1615
BsnI GGCC 7 cut(s) 678, 814, 905, 1313, 1511, 1624, 1715
Bsp143I GATC 2 cut(s) 533, 707
Bsp1720I GCTNAGC 1 cut(s) 1416
Bsp19I CCATGG 1 cut(s) 528
BspACI CCGC 4 cut(s) 580, 947, 1322, 1491
BspANI GGCC 7 cut(s) 678, 814, 905, 1313, 1511, 1624, 1715
BspCNI CTCAG 9 cut(s) 460, 511, 541, 980, 1171, 1375, 1388, 1408, 1573
BspHI TCATGA 1 cut(s) 289
BspMAI CTGCAG 5 cut(s) 448, 745, 1153, 1357, 1555
BspPI GGATC 2 cut(s) 541, 715
BspQI GCTCTTC 1 cut(s) 498
BsrDI GCAATG 4 cut(s) 1057, 1261, 1459, 1663
BsrFI RCCGGY 1 cut(s) 54
BsrI ACTGG 3 cut(s) 95, 764, 1544
BssAI RCCGGY 1 cut(s) 54
BssECI CCNNGG 5 cut(s) 528, 906, 1314, 1512, 1716
BssMI GATC 2 cut(s) 533, 707
BssT1I CCWWGG 5 cut(s) 528, 906, 1314, 1512, 1716
Bst2UI CCWGG 2 cut(s) 705, 1773
Bst4CI ACNGT 9 cut(s) 151, 216, 399, 412, 559, 879, 1083, 1485, 1689
Bst6I CTCTTC 2 cut(s) 498, 588
BstDEI CTNAG 9 cut(s) 468, 519, 549, 967, 1179, 1375, 1383, 1416, 1581
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 3 cut(s) 524, 1069, 1471
BstKTI GATC 2 cut(s) 536, 710
BstMAI GTCTC 1 cut(s) 1692
BstMBI GATC 2 cut(s) 533, 707
BstMWI GCNNNNNNNGC 6 cut(s) 11, 230, 488, 497, 760, 1319
BstNI CCWGG 2 cut(s) 705, 1773
BstSCI CCNGG 2 cut(s) 703, 1771
BstSFI CTRYAG 5 cut(s) 444, 741, 1149, 1353, 1551
BstV2I GAAGAC 2 cut(s) 1122, 1728
BstXI CCANNNNNNTGG 1 cut(s) 913
BsuRI GGCC 7 cut(s) 678, 814, 905, 1313, 1511, 1624, 1715
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 3 cut(s) 524, 1069, 1471
BtsI GCAGTG 1 cut(s) 1283
CaiI CAGNNNCTG 4 cut(s) 224, 1178, 1382, 1550
CciI TCATGA 1 cut(s) 289
Cfr10I RCCGGY 1 cut(s) 54
Cfr13I GGNCC 6 cut(s) 119, 676, 812, 1622, 1742, 1775
CseI GACGC 1 cut(s) 1753
Csp6I GTAC 2 cut(s) 392, 408
CspCI CAANNNNNGTGG 6 cut(s) 1039, 1074, 1243, 1278, 1645, 1680
CviQI GTAC 2 cut(s) 392, 408
DdeI CTNAG 9 cut(s) 468, 519, 549, 967, 1179, 1375, 1383, 1416, 1581
DpnI GATC 2 cut(s) 535, 709
DpnII GATC 2 cut(s) 533, 707
DraI TTTAAA 1 cut(s) 22
DriI GACNNNNNGTC 2 cut(s) 970, 1378
EaeI YGGCCR 4 cut(s) 903, 1311, 1509, 1713
Eam1104I CTCTTC 2 cut(s) 498, 588
Eam1105I GACNNNNNGTC 2 cut(s) 970, 1378
EarI CTCTTC 2 cut(s) 498, 588
Eco130I CCWWGG 5 cut(s) 528, 906, 1314, 1512, 1716
Eco47I GGWCC 3 cut(s) 119, 1742, 1775
Eco57I CTGAAG 8 cut(s) 124, 246, 705, 810, 1014, 1218, 1416, 1620
EcoO109I RGGNCCY 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 2 cut(s) 703, 1771
EcoT14I CCWWGG 5 cut(s) 528, 906, 1314, 1512, 1716
EcoT22I ATGCAT 1 cut(s) 275
ErhI CCWWGG 5 cut(s) 528, 906, 1314, 1512, 1716
FblI GTMKAC 1 cut(s) 146
FokI GGATG 3 cut(s) 511, 1076, 1478
FspBI CTAG 2 cut(s) 483, 497
GsuI CTGGAG 1 cut(s) 1716
HaeIII GGCC 7 cut(s) 678, 814, 905, 1313, 1511, 1624, 1715
HapII CCGG 1 cut(s) 55
HgaI GACGC 1 cut(s) 1753
HincII GTYRAC 3 cut(s) 555, 1227, 1425
HindII GTYRAC 3 cut(s) 555, 1227, 1425
HindIII AAGCTT 1 cut(s) 951
HinfI GANTC 8 cut(s) 176, 464, 521, 722, 965, 1169, 1373, 1571
HpaI GTTAAC 2 cut(s) 555, 1425
HpaII CCGG 1 cut(s) 55
HphI GGTGA 6 cut(s) 269, 826, 1030, 1234, 1432, 1636
Hpy166II GTNNAC 5 cut(s) 147, 192, 555, 1227, 1425
Hpy188III TCNNGA 7 cut(s) 49, 290, 320, 415, 1293, 1695, 1740
Hpy8I GTNNAC 5 cut(s) 147, 192, 555, 1227, 1425
HpyAV CCTTC 4 cut(s) 110, 132, 144, 148
HpyCH4III ACNGT 9 cut(s) 151, 216, 399, 412, 559, 879, 1083, 1485, 1689
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 230, 488, 497, 760, 1319
HpyF3I CTNAG 9 cut(s) 468, 519, 549, 967, 1179, 1375, 1383, 1416, 1581
KspAI GTTAAC 2 cut(s) 555, 1425
Kzo9I GATC 2 cut(s) 533, 707
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 1 cut(s) 440
LweI GCATC 3 cut(s) 250, 282, 1551
MaeI CTAG 2 cut(s) 483, 497
MaeIII GTNAC 1 cut(s) 1479
MalI GATC 2 cut(s) 535, 709
MboI GATC 2 cut(s) 533, 707
MboII GAAGA 8 cut(s) 346, 372, 485, 605, 677, 785, 1122, 1728
MlsI TGGCCA 2 cut(s) 905, 1313
MluCI AATT 4 cut(s) 17, 294, 340, 426
MluNI TGGCCA 2 cut(s) 905, 1313
MlyI GAGTC 4 cut(s) 170, 530, 959, 1367
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 8 cut(s) 363, 630, 1174, 1300, 1378, 1576, 1702, 1747
Mox20I TGGCCA 2 cut(s) 905, 1313
Mph1103I ATGCAT 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 777
MscI TGGCCA 2 cut(s) 905, 1313
Msp20I TGGCCA 2 cut(s) 905, 1313
MspA1I CMGCKG 2 cut(s) 209, 221
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 2 cut(s) 705, 1773
Mva1269I GAATGC 1 cut(s) 1615
MvaI CCWGG 2 cut(s) 705, 1773
MwoI GCNNNNNNNGC 6 cut(s) 11, 230, 488, 497, 760, 1319
NcoI CCATGG 1 cut(s) 528
NdeII GATC 2 cut(s) 533, 707
NmuCI GTSAC 1 cut(s) 1479
NsiI ATGCAT 1 cut(s) 275
PagI TCATGA 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 498
PctI GAATGC 1 cut(s) 1615
PdmI GAANNNNTTC 1 cut(s) 777
PfeI GAWTC 4 cut(s) 464, 722, 1169, 1571
PleI GAGTC 4 cut(s) 170, 529, 959, 1367
PpsI GAGTC 4 cut(s) 170, 529, 959, 1367
PpuMI RGGWCCY 1 cut(s) 119
Psp5II RGGWCCY 1 cut(s) 119
Psp6I CCWGG 2 cut(s) 703, 1771
PspGI CCWGG 2 cut(s) 703, 1771
PspPI GGNCC 6 cut(s) 119, 676, 812, 1622, 1742, 1775
PspPPI RGGWCCY 1 cut(s) 119
PstI CTGCAG 5 cut(s) 448, 745, 1153, 1357, 1555
PstNI CAGNNNCTG 4 cut(s) 224, 1178, 1382, 1550
PvuII CAGCTG 2 cut(s) 209, 221
RsaI GTAC 2 cut(s) 393, 409
RsaNI GTAC 2 cut(s) 392, 408
SapI GCTCTTC 1 cut(s) 498
Sau3AI GATC 2 cut(s) 533, 707
Sau96I GGNCC 6 cut(s) 119, 676, 812, 1622, 1742, 1775
SchI GAGTC 4 cut(s) 170, 530, 959, 1367
ScrFI CCNGG 2 cut(s) 705, 1773
SfaNI GCATC 3 cut(s) 250, 282, 1551
SfcI CTRYAG 5 cut(s) 444, 741, 1149, 1353, 1551
SinI GGWCC 3 cut(s) 119, 1742, 1775
Sse9I AATT 4 cut(s) 17, 294, 340, 426
SsiI CCGC 4 cut(s) 580, 947, 1322, 1491
SspI AATATT 1 cut(s) 1201
SspMI CTAG 2 cut(s) 483, 497
StyD4I CCNGG 2 cut(s) 703, 1771
StyI CCWWGG 5 cut(s) 528, 906, 1314, 1512, 1716
TaaI ACNGT 9 cut(s) 151, 216, 399, 412, 559, 879, 1083, 1485, 1689
TaqI TCGA 1 cut(s) 414
TasI AATT 4 cut(s) 17, 294, 340, 426
TatI WGTACW 1 cut(s) 407
TfiI GAWTC 4 cut(s) 464, 722, 1169, 1571
TseFI GTSAC 1 cut(s) 1479
Tsp45I GTSAC 1 cut(s) 1479
TspDTI ATGAA 7 cut(s) 278, 306, 353, 393, 621, 678, 735
VpaK11BI GGWCC 3 cut(s) 119, 1742, 1775
XapI RAATTY 2 cut(s) 17, 426
XcmI CCANNNNNNNNNTGG 1 cut(s) 686
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 777
XspI CTAG 2 cut(s) 483, 497
Zsp2I ATGCAT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.