AT1G15350

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003996
Physical Location & Seq
Reverse (-)
5278027 .. 5279988
1962 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G15350.4

Sequence Viewer

Length: 465 bp
ATGGGTGGCTGCGTGGGTTGCTACAGAGAACACAGATCAACTGCCGCCAGTCTGAAGGATCCACCTTCCAATTCCATTGCTCGACCGTGTAAGAAACCGAGTGTCTCTGAAGATTTCTGGTCAACAAGCACAGTTGATATGGACAACATCACCTTCCCTTCACAGGGGAGCTTATCATCATCCAATCAGACTTTTGATTCTCAATCTGCTGCTAGAAACTCTAATGCTCCTCCTGAATATGTAAATCAAGGTCTTCTTCTTTGGAATCAGACACGGGAGCGTTGGGTGGGAAAGGATAAACCCAACAACCCGGTGGATCACAATCAAGGAGCCAAGTTAAATTGGAACACAGCAACGTATGATAGCTTGCTGGGGAGCAACAAGTTATTTCCCCAACCCATTCCTCTCACCGAAATGGTGGATTTTCTAGTGGACATTTGGGAACAAGAAGGTCTATATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

154

Amino Acids

17.16

Weight (kDa)

4.95

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 8 - 62 5.7e-09 Gag1-like, clamp domain
clamp_Gag1-like PF13259 66 - 108 1.5e-12 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 45
AclWI GGATC 3 cut(s) 53, 66, 324
AcuI CTGAAG 2 cut(s) 74, 129
AfiI CCNNNNNNNGG 2 cut(s) 163, 164
AluBI AGCT 2 cut(s) 171, 366
AluI AGCT 2 cut(s) 171, 366
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 3 cut(s) 53, 66, 324
ApeKI GCWGC 2 cut(s) 9, 209
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 2 cut(s) 142, 400
BamHI GGATCC 1 cut(s) 58
BbsI GAAGAC 1 cut(s) 245
BbvI GCAGC 1 cut(s) 196
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 2 cut(s) 213, 428
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 3 cut(s) 10, 45, 210
BlsI GCNGC 3 cut(s) 11, 46, 211
Bme1390I CCNGG 1 cut(s) 311
BmiI GGNNCC 2 cut(s) 60, 331
BmrFI CCNGG 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 321
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 164
Bse1I ACTGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 75
Bse8I GATNNNNATC 1 cut(s) 321
BseGI GGATG 1 cut(s) 179
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 163, 164
BseMI GCAATG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 219
BseXI GCAGC 1 cut(s) 196
BseYI CCCAGC 1 cut(s) 370
Bsh1285I CGRYCG 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 86
BsiSI CCGG 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 163, 164
BsmAI GTCTC 1 cut(s) 109
Bsp143I GATC 3 cut(s) 35, 58, 316
BspACI CCGC 1 cut(s) 45
BspLI GGNNCC 2 cut(s) 60, 331
BspPI GGATC 3 cut(s) 53, 66, 324
BsrDI GCAATG 1 cut(s) 75
BsrI ACTGG 1 cut(s) 48
BssMI GATC 3 cut(s) 35, 58, 316
Bst4CI ACNGT 2 cut(s) 87, 133
BstC8I GCNNGC 1 cut(s) 368
BstF5I GGATG 1 cut(s) 179
BstKTI GATC 3 cut(s) 38, 61, 319
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 3 cut(s) 35, 58, 316
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 309
BstSFI CTRYAG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BtsCI GGATG 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 368
CviJI RGCY 4 cut(s) 9, 171, 332, 366
CviKI_1 RGCY 4 cut(s) 9, 171, 332, 366
DpnI GATC 3 cut(s) 37, 60, 318
DpnII GATC 3 cut(s) 35, 58, 316
Eco57I CTGAAG 2 cut(s) 74, 129
FaiI YATR 5 cut(s) 140, 240, 360, 457, 459
FalI AAGNNNNNCTT 2 cut(s) 240, 272
Fnu4HI GCNGC 3 cut(s) 10, 45, 210
FokI GGATG 1 cut(s) 166
Fsp4HI GCNGC 3 cut(s) 10, 45, 210
FspBI CTAG 2 cut(s) 213, 428
GluI GCNGC 3 cut(s) 10, 45, 210
GsaI CCCAGC 1 cut(s) 374
HapII CCGG 1 cut(s) 311
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 2 cut(s) 197, 265
HpaII CCGG 1 cut(s) 311
HphI GGTGA 2 cut(s) 142, 400
Hpy166II GTNNAC 2 cut(s) 123, 433
Hpy188I TCNGA 4 cut(s) 54, 109, 189, 270
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 2 cut(s) 123, 433
HpyAV CCTTC 5 cut(s) 49, 75, 163, 168, 443
HpyCH4III ACNGT 2 cut(s) 87, 133
HpyCH4IV ACGT 1 cut(s) 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 18
HpySE526I ACGT 1 cut(s) 356
Kzo9I GATC 3 cut(s) 35, 58, 316
LmnI GCTCC 5 cut(s) 168, 232, 277, 329, 375
LpnPI CCDG 6 cut(s) 61, 103, 149, 246, 324, 356
Lsp1109I GCAGC 1 cut(s) 196
MaeI CTAG 2 cut(s) 213, 428
MaeII ACGT 1 cut(s) 356
MalI GATC 3 cut(s) 37, 60, 318
MboI GATC 3 cut(s) 35, 58, 316
MboII GAAGA 3 cut(s) 122, 245, 248
MflI RGATCY 1 cut(s) 58
MluCI AATT 2 cut(s) 70, 340
MnlI CCTC 2 cut(s) 240, 414
MseI TTAA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 413
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 18
NciI CCSGG 1 cut(s) 311
NdeII GATC 3 cut(s) 35, 58, 316
NlaIV GGNNCC 2 cut(s) 60, 331
PfeI GAWTC 2 cut(s) 197, 265
PkrI GCNGC 3 cut(s) 11, 46, 211
PspFI CCCAGC 1 cut(s) 370
PspN4I GGNNCC 2 cut(s) 60, 331
PsuI RGATCY 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 3 cut(s) 10, 45, 210
Sau3AI GATC 3 cut(s) 35, 58, 316
ScrFI CCNGG 1 cut(s) 311
SetI ASST 7 cut(s) 67, 155, 173, 253, 359, 368, 454
SfcI CTRYAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 2 cut(s) 70, 340
SsiI CCGC 1 cut(s) 45
SspMI CTAG 2 cut(s) 213, 428
StyD4I CCNGG 1 cut(s) 309
TaaI ACNGT 2 cut(s) 87, 133
TaiI ACGT 1 cut(s) 359
TaqI TCGA 1 cut(s) 82
TasI AATT 2 cut(s) 70, 340
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 197, 265
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TseI GCWGC 2 cut(s) 9, 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XspI CTAG 2 cut(s) 213, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.