Rroxscaffold_7G00178850

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003996
Physical Location & Seq
Forward (+)
18494363 .. 18498406
4044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00178850.1

Sequence Viewer

Length: 474 bp
ATGCATGGGAGCAGAGGTTGTCTTGGAAGCTGTAGCCAACCCAGATTTACTGTGGTAGATGAGCCATCAAGCGGACTGAGAATTCAAGGTCAACCAGTGAGGAAAAACAGCATGTCAGAGGATTTCTGGAGCACTAGCACTATTGAAATGGATAACAGTGCGATTCAGTCTCAGATAAGCATCTCATCAATTAGCACTTCAAACAATCCCCTTGACGCCTGTGGTGCGGGCAGCACAAGCAATACTCCTGAATTTGTAAATCACGGTCTCTTACTCTGGAACCAGACAAGACAGCAGTGGTGTGGAAATAAAAAGTCTCGACCCCAGACACAACTTCGAGAACCTACAATAAGTTGGAATGCAACTTATGAGAGCTTACTTAGTACCAACAAGCCCTTTCGCCAGCCAATCCCTCTTCCTGAAATGATCGATTTTCTTGTTGATATATGGGAGCAAGAGGGTTTGTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

157

Amino Acids

17.57

Weight (kDa)

5.14

Isoelectric Point (pI)

60.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 47 - 157 3.1e-25 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 72, 227
AcsI RAATTY 2 cut(s) 81, 251
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 1 cut(s) 385
AfiI CCNNNNNNNGG 1 cut(s) 71
AgsI TTSAA 3 cut(s) 86, 146, 201
AluBI AGCT 2 cut(s) 30, 375
AluI AGCT 2 cut(s) 30, 375
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 3 cut(s) 174, 272, 321
ApeKI GCWGC 1 cut(s) 231
ApoI RAATTY 2 cut(s) 81, 251
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 243
BccI CCATC 1 cut(s) 73
BcoDI GTCTC 3 cut(s) 174, 272, 321
BfaI CTAG 1 cut(s) 135
BfmI CTRYAG 1 cut(s) 31
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 281
BmsI GCATC 1 cut(s) 189
BpmI CTGGAG 1 cut(s) 148
Bsa29I ATCGAT 1 cut(s) 429
BsaBI GATNNNNATC 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 272
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 95
Bse8I GATNNNNATC 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 429
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMII CTCAG 2 cut(s) 68, 185
BseNI ACTGG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 243
BshVI ATCGAT 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 3 cut(s) 174, 272, 321
BsmI GAATGC 1 cut(s) 364
Bso31I GGTCTC 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 1 cut(s) 426
BspACI CCGC 2 cut(s) 72, 227
BspCNI CTCAG 2 cut(s) 69, 184
BspDI ATCGAT 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 272
BsrI ACTGG 1 cut(s) 95
BssMI GATC 1 cut(s) 426
BssNI GRCGYC 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 52, 158, 266
Bst6I CTCTTC 1 cut(s) 420
BstACI GRCGYC 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 229, 404
BstDEI CTNAG 3 cut(s) 77, 171, 380
BstKTI GATC 1 cut(s) 429
BstMAI GTCTC 3 cut(s) 174, 272, 321
BstMBI GATC 1 cut(s) 426
BstMWI GCNNNNNNNGC 2 cut(s) 224, 237
BstNSI RCATGY 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 243
Bsu15I ATCGAT 1 cut(s) 429
BsuTUI ATCGAT 1 cut(s) 429
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 3 cut(s) 102, 163, 302
Cac8I GCNNGC 2 cut(s) 229, 404
ClaI ATCGAT 1 cut(s) 429
CseI GACGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 384
CviAII CATG 2 cut(s) 5, 112
CviJI RGCY 6 cut(s) 30, 36, 64, 375, 394, 406
CviKI_1 RGCY 6 cut(s) 30, 36, 64, 375, 394, 406
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 3 cut(s) 77, 171, 380
DpnI GATC 1 cut(s) 428
DpnII GATC 1 cut(s) 426
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 8, 115
FaiI YATR 6 cut(s) 6, 113, 369, 446, 448, 468
FatI CATG 2 cut(s) 4, 111
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 232
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 1 cut(s) 135
GluI GCNGC 1 cut(s) 232
GsuI CTGGAG 1 cut(s) 148
HgaI GACGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 2 cut(s) 8, 115
HincII GTYRAC 1 cut(s) 92
HindII GTYRAC 1 cut(s) 92
HinfI GANTC 1 cut(s) 163
Hpy166II GTNNAC 1 cut(s) 92
Hpy188I TCNGA 2 cut(s) 118, 174
Hpy188III TCNNGA 6 cut(s) 127, 248, 277, 318, 338, 419
Hpy8I GTNNAC 1 cut(s) 92
HpyCH4III ACNGT 3 cut(s) 52, 158, 266
HpyCH4V TGCA 2 cut(s) 4, 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 237
HpyF3I CTNAG 3 cut(s) 77, 171, 380
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 8, 115
Kzo9I GATC 1 cut(s) 426
LmnI GCTCC 3 cut(s) 9, 129, 451
Lsp1109I GCAGC 1 cut(s) 243
LweI GCATC 1 cut(s) 189
MaeI CTAG 1 cut(s) 135
MalI GATC 1 cut(s) 428
MboI GATC 1 cut(s) 426
MboII GAAGA 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 3 cut(s) 81, 189, 251
MmeI TCCRAC 1 cut(s) 335
MnlI CCTC 5 cut(s) 8, 93, 112, 423, 451
Mph1103I ATGCAT 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 364
MwoI GCNNNNNNNGC 2 cut(s) 224, 237
NdeII GATC 1 cut(s) 426
NlaIII CATG 2 cut(s) 8, 115
NlaIV GGNNCC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 115
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 281
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SatI GCNGC 1 cut(s) 232
Sau3AI GATC 1 cut(s) 426
SduI GDGCHC 1 cut(s) 134
SetI ASST 5 cut(s) 19, 32, 91, 346, 377
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 1 cut(s) 31
Sse9I AATT 3 cut(s) 81, 189, 251
SsiI CCGC 2 cut(s) 72, 227
SspMI CTAG 1 cut(s) 135
TaaI ACNGT 3 cut(s) 52, 158, 266
TaqI TCGA 3 cut(s) 319, 337, 429
TasI AATT 3 cut(s) 81, 189, 251
TfiI GAWTC 1 cut(s) 163
TscAI CASTG 3 cut(s) 102, 163, 302
TseI GCWGC 1 cut(s) 231
TspRI CASTG 3 cut(s) 102, 163, 302
XapI RAATTY 2 cut(s) 81, 251
XceI RCATGY 1 cut(s) 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XspI CTAG 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.