Rorug06G0277700

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003996
Physical Location & Seq
Forward (+)
42537133 .. 42538860
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0277700.1

Sequence Viewer

Length: 1728 bp
ATGGCGTCTTCATGTGTTCCTGGTCTTAATCTTCCATCGGGTTTTAAGTTTCTTCCAACCAACGAAGAACTATTGGGATATTATCTTCTGAACAAGGTCCGCGGCAAACCATTCAAGTATGACTCTAGGGTTATGAATGAGTTCAATCTTTACAAGACGGAACCCTGGGATCTTTGGACTAGGTTTGGCGGCCCAAGACTGAACCAGGGTGAAGACCTCTTTATCTTTACCGAATTGAAGAAGGTGAATGATAATGGATCAAACGTGTCTCGCACGGCAGGTTCTGGCACCTGGAAAGGTGACACTGCAGGGGCCAAAGTATATGATTCAGAGGATAAGAAGAAGATCTTAGGATCGTGGAAAAGGTTTCATTACGTGCCGAATGAACCAAATGAGAATGGTTCTTGGATTATGTTCGAGTACAAGCTTGATGATTCACTGATCCCCAAATCTTGTAGTGGTCGTGATATTGTGCTGTGCCGGATTCGGAAAGACCGAAAGAGAAAGTTGATTGAAGATCAGATGAATGACACTCCTCAGTCCTCCTGTAGAAAGGTTCCAAGGGTAGACAAACATGATGTCATGTCTACTCATGGGAAACATATTATGAATGATGATACTAATCTGCCGGCTTCTTTTACTCAAGGCTTAGGTAGTGAGGATGAGGAGCAACAATCACAACAACGACAACAACTTGATTCTAACAAGAGCTTTGAGGAACAAGAAATACAACAAGAAGATCCGCAACCGAGAAGGCAACATTCTCATTTTCATGAGCGGCAGCAGCAGCTCCTCGGCAATGATCTGATGATCTCAAGTGATCATTACATATTTCAAAGGGGAGAAACTTATGATCCTATGAATATTGTTCGACCTAACCCTAATGCTGTCATCAGTCATCAGCCGAACAATCTTGGCATTCCTTCTTATTTTCATGAGCAGCAGCAGCAGCTCCTTAGTGATGATCTGATGGTTTCTAGTCATCTTGACAAATTTCAGAGGGTAGACAAACAAGAATCTTATGTCATGGCCCCTTACGAGAATCAGATTTTGAATGATGAAGATTATAACCTCATGGCTTCATCTTTTACTCGATGGTTATCCAGTGATGAGGAAAAGCAGCAACCACAACAACAAGAAGAAGTCGAACCTAACAATATGTGTGCCGGACAGCAGTACTCCTCGGTGAGCTTTGAGCGTGAGGAACAACAACTACAACAACAAGATCAGCAACTGCTGCCCTCTACCTCTGATTGTCATGAGAAACAGCAACAGCTCCACGGCAATGATCTGATGGTTTTTAGTGATCATGACATATTTCGAAGGGCGGAAACATATGAACCTATGAATGTTCAACCTAACCCAGATACCCTCATCAGTCATCAGCCGAACCATCTTAGCATTCCCATTGACATAGATGCAATCCAATTTGATGATTATTGGACGCCGAATGAATTACTTTTGGTTGACACCAATAATGATCCTTATACCGCGGCACCTGATTCAGTACTAGACACATCAAAGCCGGTGCAGATGCAAGATTCTGCAGCTAATGATAACAACGAGATGATGAGGATTGATGAGAGTCATGATCAGACTCAACTGGGTTTCCCTCATGATTCAAACACGACGGAACTTTCTGACAGTGCTGCCCATAATGATGCGCTCAACTTTAGCACCCTTGATGACAATGTGTTCAATTTTATTGATCTTGACAACCTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

575

Amino Acids

66.49

Weight (kDa)

4.71

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 12 - 143 1.2e-23 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1068
Acc36I ACCTGC 1 cut(s) 269
AccB1I GGYRCC 2 cut(s) 287, 1495
AccBSI CCGCTC 1 cut(s) 778
AccI GTMKAC 3 cut(s) 567, 587, 1005
AccII CGCG 2 cut(s) 102, 1493
AciI CCGC 8 cut(s) 100, 102, 189, 743, 778, 1328, 1491, 1493
AclWI GGATC 7 cut(s) 177, 265, 361, 436, 734, 848, 1475
AcsI RAATTY 1 cut(s) 992
AcyI GRCGYC 2 cut(s) 5, 1445
AfaI GTAC 3 cut(s) 422, 1178, 1509
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 9 cut(s) 115, 145, 238, 515, 836, 1054, 1355, 1623, 1699
AjnI CCWGG 4 cut(s) 19, 164, 204, 290
AluBI AGCT 7 cut(s) 427, 711, 790, 952, 1191, 1276, 1550
AluI AGCT 7 cut(s) 427, 711, 790, 952, 1191, 1276, 1550
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 7 cut(s) 177, 265, 361, 436, 734, 848, 1475
AlwNI CAGNNNCTG 2 cut(s) 284, 1234
AoxI GGCC 3 cut(s) 190, 312, 1029
ApoI RAATTY 1 cut(s) 992
ArsI GACNNNNNNTTYG 2 cut(s) 564, 596
Asp700I GAANNNNTTC 1 cut(s) 140
AspLEI GCGC 1 cut(s) 1666
AspS9I GGNCC 4 cut(s) 97, 191, 312, 1030
AsuHPI GGTGA 4 cut(s) 221, 256, 311, 1198
AsuII TTCGAA 1 cut(s) 1321
AvaII GGWCC 1 cut(s) 97
BanI GGYRCC 2 cut(s) 287, 1495
BbsI GAAGAC 1 cut(s) 219
BccI CCATC 5 cut(s) 43, 964, 1089, 1288, 1401
BceAI ACGGC 2 cut(s) 291, 1297
BciT130I CCWGG 4 cut(s) 21, 166, 206, 292
BclI TGATCA 3 cut(s) 820, 1306, 1591
BcoDI GTCTC 1 cut(s) 273
BfaI CTAG 4 cut(s) 126, 180, 978, 1511
BfmI CTRYAG 3 cut(s) 306, 547, 1545
BfuAI ACCTGC 1 cut(s) 269
BglII AGATCT 1 cut(s) 345
BmcAI AGTACT 2 cut(s) 1178, 1509
Bme1390I CCNGG 4 cut(s) 21, 166, 206, 292
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 4 cut(s) 97, 191, 312, 1030
BmiI GGNNCC 6 cut(s) 162, 289, 313, 558, 1032, 1497
BmrFI CCNGG 4 cut(s) 21, 166, 206, 292
BmrI ACTGGG 1 cut(s) 1613
BmsI GCATC 3 cut(s) 1408, 1524, 1651
BmuI ACTGGG 1 cut(s) 1613
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 649
Bpu14I TTCGAA 1 cut(s) 1321
BpuEI CTTGAG 2 cut(s) 627, 799
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 2 cut(s) 621, 1099
BsaHI GRCGYC 2 cut(s) 5, 1445
BsaJI CCNNGG 9 cut(s) 100, 164, 165, 205, 560, 793, 1182, 1279, 1491
Bse118I RCCGGY 2 cut(s) 628, 1525
Bse1I ACTGG 2 cut(s) 1104, 1608
Bse3DI GCAATG 2 cut(s) 805, 1291
Bse8I GATNNNNATC 2 cut(s) 621, 1099
BseBI CCWGG 4 cut(s) 21, 166, 206, 292
BseDI CCNNGG 9 cut(s) 100, 164, 165, 205, 560, 793, 1182, 1279, 1491
BseGI GGATG 1 cut(s) 667
BseJI GATNNNNATC 2 cut(s) 621, 1099
BseMI GCAATG 2 cut(s) 805, 1291
BseMII CTCAG 1 cut(s) 551
BseNI ACTGG 2 cut(s) 1104, 1608
BseRI GAGGAG 4 cut(s) 525, 680, 782, 1171
BsgI GTGCAG 1 cut(s) 1550
Bsh1236I CGCG 2 cut(s) 102, 1493
BshFI GGCC 3 cut(s) 192, 314, 1031
BshNI GGYRCC 2 cut(s) 287, 1495
BsiSI CCGG 4 cut(s) 481, 629, 1167, 1526
BsmAI GTCTC 1 cut(s) 273
BsmI GAATGC 2 cut(s) 918, 1401
BsnI GGCC 3 cut(s) 192, 314, 1031
Bsp119I TTCGAA 1 cut(s) 1321
BspACI CCGC 8 cut(s) 100, 102, 189, 743, 778, 1328, 1491, 1493
BspANI GGCC 3 cut(s) 192, 314, 1031
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 2 cut(s) 102, 1493
BspHI TCATGA 6 cut(s) 772, 934, 1258, 1309, 1588, 1615
BspLI GGNNCC 6 cut(s) 162, 289, 313, 558, 1032, 1497
BspMAI CTGCAG 2 cut(s) 310, 1549
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 7 cut(s) 177, 265, 361, 436, 734, 848, 1475
BspT104I TTCGAA 1 cut(s) 1321
BspT107I GGYRCC 2 cut(s) 287, 1495
BsrBI CCGCTC 1 cut(s) 778
BsrDI GCAATG 2 cut(s) 805, 1291
BsrFI RCCGGY 2 cut(s) 628, 1525
BsrI ACTGG 2 cut(s) 1104, 1608
BssAI RCCGGY 2 cut(s) 628, 1525
BssECI CCNNGG 9 cut(s) 100, 164, 165, 205, 560, 793, 1182, 1279, 1491
BssNI GRCGYC 2 cut(s) 5, 1445
BssT1I CCWWGG 1 cut(s) 560
Bst2UI CCWGG 4 cut(s) 21, 166, 206, 292
Bst4CI ACNGT 1 cut(s) 1646
BstACI GRCGYC 2 cut(s) 5, 1445
BstAPI GCANNNNNTGC 1 cut(s) 1237
BstBAI YACGTR 1 cut(s) 376
BstBI TTCGAA 1 cut(s) 1321
BstC8I GCNNGC 1 cut(s) 630
BstDEI CTNAG 5 cut(s) 349, 537, 649, 956, 1397
BstDSI CCRYGG 3 cut(s) 100, 1279, 1491
BstF5I GGATG 1 cut(s) 667
BstFNI CGCG 2 cut(s) 102, 1493
BstHHI GCGC 1 cut(s) 1666
BstMAI GTCTC 1 cut(s) 273
BstMWI GCNNNNNNNGC 5 cut(s) 784, 787, 946, 949, 1237
BstNI CCWGG 4 cut(s) 21, 166, 206, 292
BstSCI CCNGG 4 cut(s) 19, 164, 204, 290
BstSFI CTRYAG 3 cut(s) 306, 547, 1545
BstUI CGCG 2 cut(s) 102, 1493
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 3 cut(s) 169, 345, 739
BstYI RGATCY 3 cut(s) 169, 345, 739
BsuRI GGCC 3 cut(s) 192, 314, 1031
BtgI CCRYGG 3 cut(s) 100, 1279, 1491
BtsCI GGATG 1 cut(s) 667
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 4 cut(s) 303, 437, 1111, 1651
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 630
CaiI CAGNNNCTG 2 cut(s) 284, 1234
CciI TCATGA 6 cut(s) 772, 934, 1258, 1309, 1588, 1615
CfoI GCGC 1 cut(s) 1666
Cfr10I RCCGGY 2 cut(s) 628, 1525
Cfr13I GGNCC 4 cut(s) 97, 191, 312, 1030
Cfr42I CCGCGG 2 cut(s) 103, 1494
CseI GACGC 1 cut(s) 1453
Csp6I GTAC 3 cut(s) 421, 1177, 1508
CviQI GTAC 3 cut(s) 421, 1177, 1508
DdeI CTNAG 5 cut(s) 349, 537, 649, 956, 1397
EciI GGCGGA 1 cut(s) 1343
Eco130I CCWWGG 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 97
EcoRII CCWGG 4 cut(s) 19, 164, 204, 290
EcoT14I CCWWGG 1 cut(s) 560
ErhI CCWWGG 1 cut(s) 560
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FauNDI CATATG 1 cut(s) 1336
FbaI TGATCA 3 cut(s) 820, 1306, 1591
FblI GTMKAC 3 cut(s) 567, 587, 1005
FokI GGATG 1 cut(s) 674
FspBI CTAG 4 cut(s) 126, 180, 978, 1511
GlaI GCGC 1 cut(s) 1665
HaeIII GGCC 3 cut(s) 192, 314, 1031
HapII CCGG 4 cut(s) 481, 629, 1167, 1526
HgaI GACGC 1 cut(s) 1453
HhaI GCGC 1 cut(s) 1666
Hin1I GRCGYC 2 cut(s) 5, 1445
Hin6I GCGC 1 cut(s) 1664
HinP1I GCGC 1 cut(s) 1664
HincII GTYRAC 1 cut(s) 1468
HindII GTYRAC 1 cut(s) 1468
HindIII AAGCTT 1 cut(s) 425
HpaII CCGG 4 cut(s) 481, 629, 1167, 1526
HphI GGTGA 4 cut(s) 221, 256, 311, 1198
Hpy166II GTNNAC 4 cut(s) 568, 588, 1006, 1468
Hpy188III TCNNGA 9 cut(s) 464, 773, 935, 986, 1259, 1310, 1589, 1616, 1712
Hpy8I GTNNAC 4 cut(s) 568, 588, 1006, 1468
Hpy99I CGWCG 1 cut(s) 1633
HpyAV CCTTC 4 cut(s) 235, 747, 933, 1317
HpyCH4III ACNGT 1 cut(s) 1646
HpyCH4IV ACGT 2 cut(s) 264, 375
HpyCH4V TGCA 5 cut(s) 308, 1421, 1531, 1537, 1547
HpyF10VI GCNNNNNNNGC 5 cut(s) 784, 787, 946, 949, 1237
HpyF3I CTNAG 5 cut(s) 349, 537, 649, 956, 1397
HpySE526I ACGT 2 cut(s) 264, 375
Hsp92I GRCGYC 2 cut(s) 5, 1445
HspAI GCGC 1 cut(s) 1664
KroI GCCGGC 1 cut(s) 628
KroNI GCCGGC 1 cut(s) 630
Ksp22I TGATCA 3 cut(s) 820, 1306, 1591
KspI CCGCGG 2 cut(s) 103, 1494
LmnI GCTCC 4 cut(s) 667, 795, 957, 1281
LweI GCATC 3 cut(s) 1408, 1524, 1651
MaeI CTAG 4 cut(s) 126, 180, 978, 1511
MaeII ACGT 2 cut(s) 264, 375
MaeIII GTNAC 1 cut(s) 299
MbiI CCGCTC 1 cut(s) 778
MflI RGATCY 3 cut(s) 169, 345, 739
MluCI AATT 5 cut(s) 233, 992, 1427, 1454, 1699
MlyI GAGTC 3 cut(s) 116, 1591, 1594
MmeI TCCRAC 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 628
MroXI GAANNNNTTC 1 cut(s) 140
MseI TTAA 2 cut(s) 27, 45
MslI CAYNNNNRTG 3 cut(s) 771, 1284, 1659
MspA1I CMGCKG 2 cut(s) 102, 1493
MspI CCGG 4 cut(s) 481, 629, 1167, 1526
MspR9I CCNGG 4 cut(s) 21, 166, 206, 292
Mva1269I GAATGC 2 cut(s) 918, 1401
MvaI CCWGG 4 cut(s) 21, 166, 206, 292
MvnI CGCG 2 cut(s) 102, 1493
MwoI GCNNNNNNNGC 5 cut(s) 784, 787, 946, 949, 1237
NaeI GCCGGC 1 cut(s) 630
NdeI CATATG 1 cut(s) 1336
NgoMIV GCCGGC 1 cut(s) 628
NlaIV GGNNCC 6 cut(s) 162, 289, 313, 558, 1032, 1497
NmeAIII GCCGAG 1 cut(s) 774
NmuCI GTSAC 1 cut(s) 299
NspV TTCGAA 1 cut(s) 1321
PagI TCATGA 6 cut(s) 772, 934, 1258, 1309, 1588, 1615
PasI CCCWGGG 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PctI GAATGC 2 cut(s) 918, 1401
PdiI GCCGGC 1 cut(s) 630
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 9 cut(s) 326, 434, 484, 698, 1016, 1042, 1502, 1541, 1619
PleI GAGTC 3 cut(s) 116, 1591, 1593
PpsI GAGTC 3 cut(s) 116, 1591, 1593
Ppu21I YACGTR 1 cut(s) 376
PsiI TTATAA 1 cut(s) 1068
Psp6I CCWGG 4 cut(s) 19, 164, 204, 290
PspGI CCWGG 4 cut(s) 19, 164, 204, 290
PspN4I GGNNCC 6 cut(s) 162, 289, 313, 558, 1032, 1497
PspPI GGNCC 4 cut(s) 97, 191, 312, 1030
PsrI GAACNNNNNNTAC 4 cut(s) 711, 743, 1197, 1229
PstI CTGCAG 2 cut(s) 310, 1549
PstNI CAGNNNCTG 2 cut(s) 284, 1234
PsuI RGATCY 3 cut(s) 169, 345, 739
RsaI GTAC 3 cut(s) 422, 1178, 1509
RsaNI GTAC 3 cut(s) 421, 1177, 1508
RseI CAYNNNNRTG 3 cut(s) 771, 1284, 1659
SacII CCGCGG 2 cut(s) 103, 1494
SaqAI TTAA 2 cut(s) 27, 45
Sau96I GGNCC 4 cut(s) 97, 191, 312, 1030
ScaI AGTACT 2 cut(s) 1178, 1509
SchI GAGTC 3 cut(s) 116, 1591, 1594
ScrFI CCNGG 4 cut(s) 21, 166, 206, 292
SfaNI GCATC 3 cut(s) 1408, 1524, 1651
SfcI CTRYAG 3 cut(s) 306, 547, 1545
Sfr303I CCGCGG 2 cut(s) 103, 1494
SfuI TTCGAA 1 cut(s) 1321
SgrBI CCGCGG 2 cut(s) 103, 1494
SinI GGWCC 1 cut(s) 97
SmiMI CAYNNNNRTG 3 cut(s) 771, 1284, 1659
SmlI CTYRAG 2 cut(s) 642, 814
SmoI CTYRAG 2 cut(s) 642, 814
Sse9I AATT 5 cut(s) 233, 992, 1427, 1454, 1699
SsiI CCGC 8 cut(s) 100, 102, 189, 743, 778, 1328, 1491, 1493
SspI AATATT 1 cut(s) 865
SspMI CTAG 4 cut(s) 126, 180, 978, 1511
StyD4I CCNGG 4 cut(s) 19, 164, 204, 290
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 1 cut(s) 1646
TaiI ACGT 2 cut(s) 267, 378
TaqI TCGA 5 cut(s) 417, 871, 1093, 1146, 1321
TaqII GACCGA 1 cut(s) 510
TasI AATT 5 cut(s) 233, 992, 1427, 1454, 1699
TatI WGTACW 3 cut(s) 420, 1176, 1507
TauI GCSGC 4 cut(s) 105, 192, 781, 1496
TfiI GAWTC 9 cut(s) 326, 434, 484, 698, 1016, 1042, 1502, 1541, 1619
Tru1I TTAA 2 cut(s) 27, 45
Tru9I TTAA 2 cut(s) 27, 45
TscAI CASTG 4 cut(s) 310, 444, 1111, 1651
TseFI GTSAC 1 cut(s) 299
Tsp45I GTSAC 1 cut(s) 299
TspGWI ACGGA 2 cut(s) 173, 1646
TspRI CASTG 4 cut(s) 310, 444, 1111, 1651
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 1 cut(s) 992
XmiI GTMKAC 3 cut(s) 567, 587, 1005
XmnI GAANNNNTTC 1 cut(s) 140
XspI CTAG 4 cut(s) 126, 180, 978, 1511
ZrmI AGTACT 2 cut(s) 1178, 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.