RchiOBHm_Chr6g0297991

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003996
Physical Location & Seq
Reverse (-)
59572502 .. 59576609
4108 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26748

Sequence Viewer

Length: 516 bp
ATGAAAAGCTATAGTTACTTTCCATCTTGGGTCTCTGCCATTTTCACATGCATGGGAGGTTGTCTTGGAAGCTGTAGCCAACCCAGATTTACTGTGGTAGATGAGCCATCAAGCGGACTGAGAATTCAAGGTCAACCAGTGAGGAAAAACAGCATGTCAGAGGATTTCTGGAGCACTAGCACTATTGAAATGGATAACAGTGGGATTCAGTCTCAGATAAGCATCTCATCAATTAGCACTTCAAACAATCCCCTTGACGCCCGTGGTGCGGGCAGCACAAGCAATACTCCTGAATTTGTAAACCACGGTCTCTTACTCTGGAACCAGACAAGACAGCAGTGGCGTGGAAATAAAAAGTCTCGACCCCAGACACAACTTCGAGAACCTACAATAAGTTGGAATGCAACTTATGAGAGCTTACTTAGTACCAACAAGCCCTTTCGCCAGCCAATCCCTCTTCCTGAAATGATCGATTTTCTTGTTGATATATGGGAGCAAGAGGGTTTGTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

171

Amino Acids

19.28

Weight (kDa)

5.43

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 61 - 171 6.2e-25 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 114, 269
AcsI RAATTY 2 cut(s) 123, 293
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 2 cut(s) 427, 509
AfiI CCNNNNNNNGG 2 cut(s) 113, 268
AgsI TTSAA 3 cut(s) 128, 188, 243
AluBI AGCT 3 cut(s) 9, 72, 417
AluI AGCT 3 cut(s) 9, 72, 417
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 4 cut(s) 37, 216, 314, 363
ApeKI GCWGC 1 cut(s) 273
ApoI RAATTY 2 cut(s) 123, 293
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 1 cut(s) 285
BccI CCATC 2 cut(s) 31, 115
BcoDI GTCTC 4 cut(s) 37, 216, 314, 363
BfaI CTAG 1 cut(s) 177
BfmI CTRYAG 2 cut(s) 10, 73
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 323
BmsI GCATC 1 cut(s) 231
BpmI CTGGAG 1 cut(s) 190
Bsa29I ATCGAT 1 cut(s) 471
BsaBI GATNNNNATC 1 cut(s) 221
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 2 cut(s) 37, 314
BsaJI CCNNGG 2 cut(s) 262, 304
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 268
Bse1I ACTGG 1 cut(s) 137
Bse8I GATNNNNATC 1 cut(s) 221
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 2 cut(s) 262, 304
BseJI GATNNNNATC 1 cut(s) 221
BseLI CCNNNNNNNGG 2 cut(s) 113, 268
BseMII CTCAG 2 cut(s) 110, 227
BseNI ACTGG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 285
BshVI ATCGAT 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 113, 268
BsmAI GTCTC 4 cut(s) 37, 216, 314, 363
BsmI GAATGC 1 cut(s) 406
Bso31I GGTCTC 2 cut(s) 37, 314
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 1 cut(s) 468
BspACI CCGC 2 cut(s) 114, 269
BspCNI CTCAG 2 cut(s) 111, 226
BspDI ATCGAT 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 323
BspTNI GGTCTC 2 cut(s) 37, 314
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 262, 304
BssMI GATC 1 cut(s) 468
BssNI GRCGYC 1 cut(s) 258
Bst4CI ACNGT 3 cut(s) 94, 200, 308
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 271, 446
BstDEI CTNAG 3 cut(s) 119, 213, 422
BstDSI CCRYGG 2 cut(s) 262, 304
BstKTI GATC 1 cut(s) 471
BstMAI GTCTC 4 cut(s) 37, 216, 314, 363
BstMBI GATC 1 cut(s) 468
BstMWI GCNNNNNNNGC 2 cut(s) 266, 279
BstNSI RCATGY 2 cut(s) 51, 157
BstSFI CTRYAG 2 cut(s) 10, 73
BstV1I GCAGC 1 cut(s) 285
Bsu15I ATCGAT 1 cut(s) 471
BsuTUI ATCGAT 1 cut(s) 471
BtgI CCRYGG 2 cut(s) 262, 304
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 3 cut(s) 144, 205, 344
Cac8I GCNNGC 2 cut(s) 271, 446
ClaI ATCGAT 1 cut(s) 471
CseI GACGC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 426, 508
CviAII CATG 3 cut(s) 48, 52, 154
CviJI RGCY 7 cut(s) 9, 72, 78, 106, 417, 436, 448
CviKI_1 RGCY 7 cut(s) 9, 72, 78, 106, 417, 436, 448
CviQI GTAC 2 cut(s) 426, 508
DdeI CTNAG 3 cut(s) 119, 213, 422
DpnI GATC 1 cut(s) 470
DpnII GATC 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Eco31I GGTCTC 2 cut(s) 37, 314
EcoRI GAATTC 1 cut(s) 123
EcoT22I ATGCAT 1 cut(s) 53
FaeI CATG 3 cut(s) 51, 55, 157
FaiI YATR 7 cut(s) 12, 49, 53, 155, 411, 488, 490
FatI CATG 3 cut(s) 47, 51, 153
FauI CCCGC 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 274
Fsp4HI GCNGC 1 cut(s) 274
FspBI CTAG 1 cut(s) 177
GluI GCNGC 1 cut(s) 274
GsuI CTGGAG 1 cut(s) 190
HgaI GACGC 1 cut(s) 266
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 3 cut(s) 51, 55, 157
HincII GTYRAC 1 cut(s) 134
HindII GTYRAC 1 cut(s) 134
HinfI GANTC 1 cut(s) 205
Hpy166II GTNNAC 2 cut(s) 134, 301
Hpy188I TCNGA 2 cut(s) 160, 216
Hpy188III TCNNGA 6 cut(s) 169, 290, 319, 360, 380, 461
Hpy8I GTNNAC 2 cut(s) 134, 301
HpyCH4III ACNGT 3 cut(s) 94, 200, 308
HpyCH4V TGCA 2 cut(s) 51, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 279
HpyF3I CTNAG 3 cut(s) 119, 213, 422
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 3 cut(s) 51, 55, 157
Kzo9I GATC 1 cut(s) 468
LmnI GCTCC 2 cut(s) 171, 493
LpnPI CCDG 9 cut(s) 97, 150, 154, 303, 304, 338, 380, 458, 474
Lsp1109I GCAGC 1 cut(s) 285
LweI GCATC 1 cut(s) 231
MaeI CTAG 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 14
MalI GATC 1 cut(s) 470
MboI GATC 1 cut(s) 468
MboII GAAGA 1 cut(s) 449
MhlI GDGCHC 1 cut(s) 176
MluCI AATT 3 cut(s) 123, 231, 293
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 5 cut(s) 50, 135, 154, 465, 493
Mph1103I ATGCAT 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 406
MwoI GCNNNNNNNGC 2 cut(s) 266, 279
NdeII GATC 1 cut(s) 468
NlaIII CATG 3 cut(s) 51, 55, 157
NlaIV GGNNCC 1 cut(s) 323
NsiI ATGCAT 1 cut(s) 53
NspI RCATGY 2 cut(s) 51, 157
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 205
PkrI GCNGC 1 cut(s) 275
PspN4I GGNNCC 1 cut(s) 323
RsaI GTAC 2 cut(s) 427, 509
RsaNI GTAC 2 cut(s) 426, 508
RseI CAYNNNNRTG 1 cut(s) 50
SatI GCNGC 1 cut(s) 274
Sau3AI GATC 1 cut(s) 468
SduI GDGCHC 1 cut(s) 176
SetI ASST 6 cut(s) 11, 61, 74, 133, 388, 419
SfaNI GCATC 1 cut(s) 231
SfcI CTRYAG 2 cut(s) 10, 73
SmiMI CAYNNNNRTG 1 cut(s) 50
Sse9I AATT 3 cut(s) 123, 231, 293
SsiI CCGC 2 cut(s) 114, 269
SspMI CTAG 1 cut(s) 177
TaaI ACNGT 3 cut(s) 94, 200, 308
TaqI TCGA 3 cut(s) 361, 379, 471
TasI AATT 3 cut(s) 123, 231, 293
TfiI GAWTC 1 cut(s) 205
TscAI CASTG 3 cut(s) 144, 205, 344
TseI GCWGC 1 cut(s) 273
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 144, 205, 344
XapI RAATTY 2 cut(s) 123, 293
XceI RCATGY 2 cut(s) 51, 157
XcmI CCANNNNNNNNNTGG 1 cut(s) 91
XspI CTAG 1 cut(s) 177
Zsp2I ATGCAT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.