RLG00000011545

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003996
Physical Location & Seq
Forward (+)
10419936 .. 10423567
3632 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011545

Sequence Viewer

Length: 594 bp
ATGCATGGGAGCAGAAGTTGTCTTGGAAGCTGTAGCCAACCCAGATTTTCTGTGGTAGATGAGCCATCAAGCGGATTGAGAATTCAAGGTCAACCAGTGAGGAAAAACAGCATGTCAGAGGATTTCTGGAGCACTAGCACTATTGAAATGGATAACAGTGCAATTCAGTCTCAGATAAGCATCTCATCAATTAGCACTTCAAACAATCCCCTTGACACCCGTGGTGCGGGCAGCACAAGCAATACTCCTGAATTTGTAAATCACGGTCTCTTACTCTGGAACCAGACAAGACAGCAGTGGCGTGGAAATAAAAAGTCTCGACCCCAGGCACAACTTCGAGAACCTACAATAAGTTGGAATGCAACTTATGAGAGCTTACTTAGTACCAACAAGCCCTTTCGCCAGCCAATCCCTCTTCCTCCTCTTCACCTCCCTGTAGTAATTACTCCTTGCTTGATGGGTTGCAACAAAGTCTGTTTTGGCAAAGTAAACCCAGAAACTGAAACCACCTTGGCCAGAGAAAAATGGCCGAAGGTGGTTAACTTGGAAATTGGGAAGAAGAAGAAGAAGAAGAAAAATTATAGAAGTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

198

Amino Acids

22.07

Weight (kDa)

9.89

Isoelectric Point (pI)

55.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 47 - 140 1.9e-14 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 72, 227
AcoI YGGCCR 2 cut(s) 513, 527
AcsI RAATTY 2 cut(s) 81, 251
AfaI GTAC 1 cut(s) 385
AfiI CCNNNNNNNGG 2 cut(s) 71, 226
AgsI TTSAA 3 cut(s) 86, 146, 201
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 2 cut(s) 30, 375
AluI AGCT 2 cut(s) 30, 375
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 3 cut(s) 174, 272, 321
AlwNI CAGNNNCTG 1 cut(s) 500
AoxI GGCC 2 cut(s) 513, 527
ApeKI GCWGC 1 cut(s) 231
ApoI RAATTY 2 cut(s) 81, 251
AsuHPI GGTGA 1 cut(s) 419
BalI TGGCCA 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 243
BccI CCATC 2 cut(s) 73, 451
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 3 cut(s) 174, 272, 321
BfaI CTAG 1 cut(s) 135
BfmI CTRYAG 2 cut(s) 31, 435
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 326
BmiI GGNNCC 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 1 cut(s) 189
BpmI CTGGAG 1 cut(s) 148
BsaBI GATNNNNATC 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 3 cut(s) 220, 324, 510
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 226
Bse1I ACTGG 1 cut(s) 95
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 3 cut(s) 220, 324, 510
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 2 cut(s) 71, 226
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 1 cut(s) 411
BseXI GCAGC 1 cut(s) 243
BshFI GGCC 2 cut(s) 515, 529
BsiHKAI GWGCWC 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 71, 226
BsmAI GTCTC 3 cut(s) 174, 272, 321
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 2 cut(s) 515, 529
Bso31I GGTCTC 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 134
BspACI CCGC 2 cut(s) 72, 227
BspANI GGCC 2 cut(s) 515, 529
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 272
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 220, 324, 510
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 2 cut(s) 158, 266
Bst6I CTCTTC 2 cut(s) 420, 429
BstC8I GCNNGC 2 cut(s) 229, 404
BstDEI CTNAG 2 cut(s) 171, 380
BstDSI CCRYGG 1 cut(s) 220
BstMAI GTCTC 3 cut(s) 174, 272, 321
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 115
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 2 cut(s) 31, 435
BstV1I GCAGC 1 cut(s) 243
BsuRI GGCC 2 cut(s) 515, 529
BtgI CCRYGG 1 cut(s) 220
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 3 cut(s) 102, 163, 302
Cac8I GCNNGC 2 cut(s) 229, 404
CaiI CAGNNNCTG 1 cut(s) 500
Csp6I GTAC 1 cut(s) 384
CviAII CATG 2 cut(s) 5, 112
CviJI RGCY 8 cut(s) 30, 36, 64, 375, 394, 406, 515, 529
CviKI_1 RGCY 8 cut(s) 30, 36, 64, 375, 394, 406, 515, 529
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 2 cut(s) 171, 380
EaeI YGGCCR 2 cut(s) 513, 527
Eam1104I CTCTTC 2 cut(s) 420, 429
EarI CTCTTC 2 cut(s) 420, 429
Eco130I CCWWGG 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 510
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 2 cut(s) 8, 115
FaiI YATR 4 cut(s) 6, 113, 369, 582
FatI CATG 2 cut(s) 4, 111
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 232
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 1 cut(s) 135
GluI GCNGC 1 cut(s) 232
GsuI CTGGAG 1 cut(s) 148
HaeIII GGCC 2 cut(s) 515, 529
Hin1II CATG 2 cut(s) 8, 115
HincII GTYRAC 2 cut(s) 92, 541
HindII GTYRAC 2 cut(s) 92, 541
HpaI GTTAAC 1 cut(s) 541
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 3 cut(s) 92, 490, 541
Hpy188I TCNGA 3 cut(s) 118, 174, 593
Hpy188III TCNNGA 5 cut(s) 127, 248, 277, 318, 338
Hpy8I GTNNAC 3 cut(s) 92, 490, 541
HpyAV CCTTC 1 cut(s) 526
HpyCH4III ACNGT 2 cut(s) 158, 266
HpyCH4V TGCA 4 cut(s) 4, 161, 362, 465
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 2 cut(s) 171, 380
Hsp92II CATG 2 cut(s) 8, 115
KspAI GTTAAC 1 cut(s) 541
LmnI GCTCC 2 cut(s) 9, 129
Lsp1109I GCAGC 1 cut(s) 243
LweI GCATC 1 cut(s) 189
MaeI CTAG 1 cut(s) 135
MboII GAAGA 8 cut(s) 407, 416, 568, 571, 574, 577, 580, 583
MhlI GDGCHC 1 cut(s) 134
MlsI TGGCCA 1 cut(s) 515
MluCI AATT 7 cut(s) 81, 162, 189, 251, 441, 549, 577
MluNI TGGCCA 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 335
MnlI CCTC 6 cut(s) 93, 112, 423, 429, 432, 440
Mox20I TGGCCA 1 cut(s) 515
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 515
MseI TTAA 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 515
MspR9I CCNGG 1 cut(s) 326
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 237
NlaIII CATG 2 cut(s) 8, 115
NlaIV GGNNCC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 115
PctI GAATGC 1 cut(s) 364
PkrI GCNGC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 281
PstNI CAGNNNCTG 1 cut(s) 500
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 1 cut(s) 232
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 1 cut(s) 134
SetI ASST 7 cut(s) 32, 91, 346, 377, 432, 512, 537
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 2 cut(s) 31, 435
Sse9I AATT 7 cut(s) 81, 162, 189, 251, 441, 549, 577
SsiI CCGC 2 cut(s) 72, 227
SspMI CTAG 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 158, 266
TaqI TCGA 2 cut(s) 319, 337
TasI AATT 7 cut(s) 81, 162, 189, 251, 441, 549, 577
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 3 cut(s) 102, 163, 302
TseI GCWGC 1 cut(s) 231
TspRI CASTG 3 cut(s) 102, 163, 302
XapI RAATTY 2 cut(s) 81, 251
XceI RCATGY 1 cut(s) 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XspI CTAG 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.