AT1G18950

DDT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007850
Physical Location & Seq
Forward (+)
6546177 .. 6552108
5932 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18950.1

Sequence Viewer

Length: 2253 bp
ATGGGTTCCTCCTCCGATATCGTCCCTGACCGATCTCCAGCTGACGATGTAGCTCCGGTTACAGATACCAAAATTCCAAAGGAGGAGCCTTTGACGCTTAGGAGAACGCGTCCTTCTCGGGCTTGTACTGTTAGGGCTCAGCAGCGGCTTCAAGAGCTTCAAGCGGCGGAGAGAAAGCTTAAGCCGCCTAAGAAGGAGTATAAACGAGAGCAGCATCGACGGAGAGAAGAGGTGGTGGAGGAAGATGAGGATTCGGAGGATGACGATCAAGAGGATGAAGAGAACGACGGAGATGATGAGAGTAATCCGAAGCAATGCGTTGCTGGAGGAAGTTCTACTAAAATCATTACTTCTCTTGTTCCGCCACCGGAACCTTCACAGATGCCGCGGTGGAATCTTCGTTCTATGTGGGAACTTGCTTCTGTGCTCAATTTCTTGCATGTATTTAGACCGCTTTTGAAAATCAATGCGGAGTTTTCAGCGGAGGAGTTTGAAACGGCTTTGCTTACTCCAAACGATACTTTAAGTGATATTCACATTCCTCTGCTAAAGGCCATTCCGCCTGTAACTCGAATGGCTCTCACCCGTGATACCTGGGTAACTGTCCTGTGCAGAAAAATAAGAGATTGCTGGCATTGGGTTGCAGAAGGGGACCTTCCTATTGTTGCCTTGCAAGGGCGGGAGATTGAAGTTTACAAAAATCTTGATCCAGCCATCCGTGTGGTGATTTTAAAAGCGTTATGTGATATTCGTGTTGAGCAAGAGGATATCAGGAGCTACATTGACAACTCTCTTAAAACTGGCGTTCACCTGTCAGTTTTTCGCAAAGATCGTGTTGGGGGTGATTCACATGGAGTTAATTTTTGGTATGAGGAAGATCCCTTAATTGGTCACCGTTTATATCGGGAAATAAGGAAAGCAGAGGTGTTGAAGGTCAAAACAAAGGGTTCCAAGATTCTTCCTAACATAACATACCAATGGGAAACTGTTGCCACTAATTTCGATGAATTCCAAGATGTTTCTGAAAAACTTCTTCAGAGTAGTAGTAGAATTGAAGTTTCTCTAGGGAAGAAGTTAGTAAAAGATATGCTTCCTGAGATAGAAAAAGAACACAAGAGGAAAGAAAAATTGTTGAAAAAGCAACACAGACAGGCTCTTCTCCTTGATAACTTTGTGGTTGTTGATGGTCTTGCTGGGCGATCTCTCCGTGATAGGAAGCCAGTTAGATACACTTTTGATGATTATGATAAGTCCATAAATGATGCTATCAAGATAACAAAGAAGAAACATCCATCACCAGAACATCCTCTCCACAGAAGAGAATCTGCCAGGTTGGACGCTCTTGCGAATGGCAGATCGACCAGTTCAACCCACCCTACTGAGCCTGTGAACGATACAGCATCTGGGAGATCTTCTGATTTTGCTGATTATGATGATTTCGATGAGCATAGAGATGAATCTTTGGACAGAAGGCGCAGACAACGACCTCAACGATATTCAGCTACGGACTTTGTTGAAACTGTTTCAGACAATGAGGTAGAATTTCAAAGTGACGATGACATTTATGGGGAAGCAGTTTATGATGAAGAATATCTGAAGAAGCGTAAACAGAAAAAGCTCTCTAGCGGTTCTGAGGGAGATGAAGAAAAGGGAGATGAAGAATACAAATGGGATGAAGACAATGCTGAGTATGAGGAAGAAGAAGAAGAGGAAGAAGAAGAAGATTCCCTAAGTGCAAGCGAAGAGGATAGTGATGAACCCCGGAGGGCTAAGAAAATGCCACGGAGAGAAACTAAGTTAAGATCAAGATCAAATGATTTTCGGCCAGGCCTGAGACGAAGTAAAAGAGCCACGCGAATTGATTACCAACAATATGAGTTCTCAGACTCGGACAAAGAAGCAACAGGACTGGCAAAACGTAAGCGGTTTGTTGAGCCGGACGAACCTTCTGATGAAACAGGGAATGGGGATTTCACAATGGGAAGTCAGGACTCTGAAGAAAATGCAAACGACCCAGAAACAAAATCTGGTGAAGAAGAAGAGCCGAGAGATGTCAATGACAATGCAGATACAACAAATGGAAAAGAGAATAACCAACTTAACAAATCAAACGGCACAACAGACCAAGAAGAAGTTGAGGGTGTAGTAGGCAAAAGGCGTTACCTCGACTTAAACGAGCTGGCCCCGGTATCTGGTTTTGATGATGGCCCAAGTACAGTATTGAAGGATGATGATAAGACAGACAATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1

Protein Analysis

750

Amino Acids

86.25

Weight (kDa)

4.91

Isoelectric Point (pI)

64.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDT PF02791 141 - 186 2.9e-07 DDT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 109, 388, 1855
AclWI GGATC 2 cut(s) 701, 872
AcoI YGGCCR 1 cut(s) 1823
AcsI RAATTY 3 cut(s) 72, 1007, 1541
AcuI CTGAAG 3 cut(s) 1019, 1616, 2016
AfaI GTAC 2 cut(s) 127, 2215
AfiI CCNNNNNNNGG 3 cut(s) 675, 887, 2192
AflII CTTAAG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 107
AjnI CCWGG 3 cut(s) 593, 1328, 1825
AloI GAACNNNNNNTCC 4 cut(s) 385, 417, 1293, 1325
AluBI AGCT 8 cut(s) 41, 53, 157, 178, 777, 1502, 1618, 2179
AluI AGCT 8 cut(s) 41, 53, 157, 178, 777, 1502, 1618, 2179
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 1828
AlwI GGATC 2 cut(s) 701, 872
AlwNI CAGNNNCTG 1 cut(s) 1403
Ama87I CYCGRG 1 cut(s) 117
AoxI GGCC 5 cut(s) 552, 1823, 1828, 2181, 2206
ApeKI GCWGC 2 cut(s) 142, 211
ApoI RAATTY 3 cut(s) 72, 1007, 1541
Asp700I GAANNNNTTC 1 cut(s) 1029
AspLEI GCGC 1 cut(s) 1476
AspS9I GGNCC 3 cut(s) 652, 2182, 2207
AsuC2I CCSGG 2 cut(s) 1762, 2186
AsuHPI GGTGA 7 cut(s) 574, 736, 800, 854, 884, 1287, 2042
AvaI CYCGRG 1 cut(s) 117
AvaII GGWCC 1 cut(s) 652
BaeI ACNNNNGTAYC 2 cut(s) 582, 615
BanII GRGCYC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 1683
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 154, 223
BccI CCATC 4 cut(s) 722, 1178, 1300, 2198
BceAI ACGGC 2 cut(s) 513, 2128
BciT130I CCWGG 3 cut(s) 595, 1330, 1827
BcnI CCSGG 2 cut(s) 1762, 2186
BcoDI GTCTC 1 cut(s) 1828
BfaI CTAG 2 cut(s) 1064, 1623
BfrI CTTAAG 1 cut(s) 179
BglII AGATCT 1 cut(s) 1409
BisI GCNGC 6 cut(s) 143, 146, 165, 185, 212, 386
BlpI GCTNAGC 1 cut(s) 138
BlsI GCNGC 6 cut(s) 144, 147, 166, 186, 213, 387
Bme1390I CCNGG 5 cut(s) 595, 1330, 1762, 1827, 2186
Bme18I GGWCC 1 cut(s) 652
BmeT110I CYCGRG 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 652, 2182, 2207
BmiI GGNNCC 6 cut(s) 7, 87, 372, 653, 949, 2184
BmrFI CCNGG 5 cut(s) 595, 1330, 1762, 1827, 2186
BmsI GCATC 4 cut(s) 223, 372, 1252, 1409
BpiI GAAGAC 1 cut(s) 1683
BpmI CTGGAG 2 cut(s) 21, 345
Bpu10I CCTNAGC 1 cut(s) 98
Bpu1102I GCTNAGC 1 cut(s) 138
BpuMI CCSGG 2 cut(s) 1762, 2186
BsaBI GATNNNNATC 2 cut(s) 264, 1807
BsaJI CCNNGG 5 cut(s) 386, 594, 1760, 1781, 2184
BsaWI WCCGGW 2 cut(s) 55, 367
BsaXI ACNNNNNCTCC 4 cut(s) 94, 124, 1293, 1323
Bsc4I CCNNNNNNNGG 3 cut(s) 675, 887, 2192
Bse1I ACTGG 4 cut(s) 805, 1220, 1362, 1914
Bse3DI GCAATG 1 cut(s) 320
Bse8I GATNNNNATC 2 cut(s) 264, 1807
BseBI CCWGG 3 cut(s) 595, 1330, 1827
BseDI CCNNGG 5 cut(s) 386, 594, 1760, 1781, 2184
BseGI GGATG 7 cut(s) 265, 280, 714, 1288, 1303, 1678, 2233
BseJI GATNNNNATC 2 cut(s) 264, 1807
BseLI CCNNNNNNNGG 3 cut(s) 675, 887, 2192
BseMI GCAATG 1 cut(s) 320
BseMII CTCAG 7 cut(s) 152, 1086, 1371, 1623, 1677, 1823, 1896
BseNI ACTGG 4 cut(s) 805, 1220, 1362, 1914
BseRI GAGGAG 2 cut(s) 98, 500
BseXI GCAGC 2 cut(s) 154, 223
BseYI CCCAGC 1 cut(s) 1193
BsgI GTGCAG 1 cut(s) 631
Bsh1236I CGCG 3 cut(s) 109, 388, 1855
BshFI GGCC 5 cut(s) 554, 1825, 1830, 2183, 2208
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 5 cut(s) 56, 368, 1762, 1937, 2186
BslFI GGGAC 2 cut(s) 8, 665
BslI CCNNNNNNNGG 3 cut(s) 675, 887, 2192
BsmAI GTCTC 1 cut(s) 1828
BsmBI CGTCTC 1 cut(s) 1828
BsmFI GGGAC 2 cut(s) 8, 665
BsnI GGCC 5 cut(s) 554, 1825, 1830, 2183, 2208
BsoBI CYCGRG 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 139, 429
Bsp1720I GCTNAGC 1 cut(s) 138
BspANI GGCC 5 cut(s) 554, 1825, 1830, 2183, 2208
BspCNI CTCAG 7 cut(s) 151, 1087, 1372, 1624, 1678, 1824, 1895
BspFNI CGCG 3 cut(s) 109, 388, 1855
BspLI GGNNCC 6 cut(s) 7, 87, 372, 653, 949, 2184
BspPI GGATC 2 cut(s) 701, 872
BspQI GCTCTTC 2 cut(s) 1161, 2034
BspTI CTTAAG 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 320
BsrI ACTGG 4 cut(s) 805, 1220, 1362, 1914
BssECI CCNNGG 5 cut(s) 386, 594, 1760, 1781, 2184
Bst2UI CCWGG 3 cut(s) 595, 1330, 1827
Bst4CI ACNGT 6 cut(s) 130, 604, 896, 988, 1522, 2218
Bst6I CTCTTC 7 cut(s) 222, 273, 1161, 1312, 1701, 1737, 2034
BstAFI CTTAAG 1 cut(s) 179
BstC8I GCNNGC 3 cut(s) 632, 1738, 2181
BstDSI CCRYGG 2 cut(s) 386, 1781
BstEII GGTNACC 1 cut(s) 890
BstF5I GGATG 7 cut(s) 265, 280, 714, 1288, 1303, 1678, 2233
BstFNI CGCG 3 cut(s) 109, 388, 1855
BstHHI GCGC 1 cut(s) 1476
BstMAI GTCTC 1 cut(s) 1828
BstMWI GCNNNNNNNGC 3 cut(s) 94, 154, 184
BstNI CCWGG 3 cut(s) 595, 1330, 1827
BstNSI RCATGY 1 cut(s) 443
BstPI GGTNACC 1 cut(s) 890
BstSCI CCNGG 5 cut(s) 593, 1328, 1760, 1825, 2184
BstUI CGCG 3 cut(s) 109, 388, 1855
BstV1I GCAGC 2 cut(s) 154, 223
BstV2I GAAGAC 1 cut(s) 1683
BstX2I RGATCY 2 cut(s) 877, 1409
BstXI CCANNNNNNTGG 1 cut(s) 721
BstYI RGATCY 2 cut(s) 877, 1409
BsuRI GGCC 5 cut(s) 554, 1825, 1830, 2183, 2208
BtgI CCRYGG 2 cut(s) 386, 1781
BtsCI GGATG 7 cut(s) 265, 280, 714, 1288, 1303, 1678, 2233
Cac8I GCNNGC 3 cut(s) 632, 1738, 2181
CaiI CAGNNNCTG 1 cut(s) 1403
CfoI GCGC 1 cut(s) 1476
Cfr13I GGNCC 3 cut(s) 652, 2182, 2207
Cfr42I CCGCGG 1 cut(s) 389
CseI GACGC 3 cut(s) 98, 103, 1346
Csp6I GTAC 2 cut(s) 126, 2214
CspCI CAANNNNNGTGG 2 cut(s) 1840, 1875
CviAII CATG 2 cut(s) 440, 851
CviQI GTAC 2 cut(s) 126, 2214
DraI TTTAAA 1 cut(s) 732
EaeI YGGCCR 1 cut(s) 1823
Eam1104I CTCTTC 7 cut(s) 222, 273, 1161, 1312, 1701, 1737, 2034
EarI CTCTTC 7 cut(s) 222, 273, 1161, 1312, 1701, 1737, 2034
EciI GGCGGA 3 cut(s) 182, 351, 549
Eco147I AGGCCT 1 cut(s) 1830
Eco24I GRGCYC 1 cut(s) 139
Eco32I GATATC 2 cut(s) 19, 769
Eco47I GGWCC 1 cut(s) 652
Eco57I CTGAAG 3 cut(s) 1019, 1616, 2016
Eco88I CYCGRG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 890
EcoO109I RGGNCCY 1 cut(s) 652
EcoO65I GGTNACC 1 cut(s) 890
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 3 cut(s) 593, 1328, 1825
EcoRV GATATC 2 cut(s) 19, 769
EcoT38I GRGCYC 1 cut(s) 139
Esp3I CGTCTC 1 cut(s) 1828
FaeI CATG 2 cut(s) 443, 854
FalI AAGNNNNNCTT 4 cut(s) 639, 671, 1074, 1106
FaqI GGGAC 2 cut(s) 8, 665
FatI CATG 2 cut(s) 439, 850
FauI CCCGC 1 cut(s) 672
Fnu4HI GCNGC 6 cut(s) 143, 146, 165, 185, 212, 386
FokI GGATG 7 cut(s) 272, 287, 701, 1275, 1290, 1685, 2240
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 6 cut(s) 143, 146, 165, 185, 212, 386
FspBI CTAG 2 cut(s) 1064, 1623
GlaI GCGC 1 cut(s) 1475
GluI GCNGC 6 cut(s) 143, 146, 165, 185, 212, 386
GsaI CCCAGC 1 cut(s) 1197
GsuI CTGGAG 2 cut(s) 21, 345
HaeIII GGCC 5 cut(s) 554, 1825, 1830, 2183, 2208
HapII CCGG 5 cut(s) 56, 368, 1762, 1937, 2186
HgaI GACGC 3 cut(s) 98, 103, 1346
HhaI GCGC 1 cut(s) 1476
Hin1II CATG 2 cut(s) 443, 854
Hin6I GCGC 1 cut(s) 1474
HinP1I GCGC 1 cut(s) 1474
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 9 cut(s) 251, 394, 845, 955, 1322, 1457, 1724, 1886, 1991
HpaII CCGG 5 cut(s) 56, 368, 1762, 1937, 2186
HphI GGTGA 7 cut(s) 574, 736, 800, 854, 884, 1287, 2042
Hpy166II GTNNAC 4 cut(s) 694, 808, 1390, 1607
Hpy188III TCNNGA 9 cut(s) 152, 269, 704, 772, 905, 1094, 1270, 1806, 1988
Hpy8I GTNNAC 4 cut(s) 694, 808, 1390, 1607
Hpy99I CGWCG 2 cut(s) 222, 290
HpyAV CCTTC 9 cut(s) 123, 187, 384, 641, 665, 925, 1464, 1956, 2218
HpyCH4III ACNGT 6 cut(s) 130, 604, 896, 988, 1522, 2218
HpyCH4IV ACGT 1 cut(s) 1918
HpyCH4V TGCA 7 cut(s) 439, 612, 644, 673, 1736, 2006, 2066
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 154, 184
HpySE526I ACGT 1 cut(s) 1918
Hsp92II CATG 2 cut(s) 443, 854
HspAI GCGC 1 cut(s) 1474
KspI CCGCGG 1 cut(s) 389
LguI GCTCTTC 2 cut(s) 1161, 2034
LmnI GCTCC 3 cut(s) 58, 85, 774
Lsp1109I GCAGC 2 cut(s) 154, 223
LweI GCATC 4 cut(s) 223, 372, 1252, 1409
MaeI CTAG 2 cut(s) 1064, 1623
MaeII ACGT 1 cut(s) 1918
MaeIII GTNAC 6 cut(s) 58, 565, 598, 890, 1550, 2159
MflI RGATCY 2 cut(s) 877, 1409
MhlI GDGCHC 2 cut(s) 139, 429
MluI ACGCGT 1 cut(s) 107
MlyI GAGTC 2 cut(s) 1880, 1985
MmeI TCCRAC 1 cut(s) 1314
MroXI GAANNNNTTC 1 cut(s) 1029
MseI TTAA 9 cut(s) 180, 524, 731, 795, 858, 884, 1799, 2100, 2171
MslI CAYNNNNRTG 3 cut(s) 719, 976, 1452
MspA1I CMGCKG 4 cut(s) 41, 145, 388, 482
MspCI CTTAAG 1 cut(s) 179
MspI CCGG 5 cut(s) 56, 368, 1762, 1937, 2186
MspR9I CCNGG 5 cut(s) 595, 1330, 1762, 1827, 2186
MvaI CCWGG 3 cut(s) 595, 1330, 1827
MvnI CGCG 3 cut(s) 109, 388, 1855
MwoI GCNNNNNNNGC 3 cut(s) 94, 154, 184
NciI CCSGG 2 cut(s) 1762, 2186
NlaIII CATG 2 cut(s) 443, 854
NlaIV GGNNCC 6 cut(s) 7, 87, 372, 653, 949, 2184
NmeAIII GCCGAG 1 cut(s) 2070
NmuCI GTSAC 2 cut(s) 890, 1550
NspI RCATGY 1 cut(s) 443
PceI AGGCCT 1 cut(s) 1830
PciSI GCTCTTC 2 cut(s) 1161, 2034
PcsI WCGNNNNNNNCGW 3 cut(s) 829, 1489, 2172
PdmI GAANNNNTTC 1 cut(s) 1029
PfeI GAWTC 7 cut(s) 251, 394, 845, 955, 1322, 1457, 1724
PkrI GCNGC 6 cut(s) 144, 147, 166, 186, 213, 387
PleI GAGTC 2 cut(s) 1880, 1985
PpsI GAGTC 2 cut(s) 1880, 1985
PpuMI RGGWCCY 1 cut(s) 652
Psp5II RGGWCCY 1 cut(s) 652
Psp6I CCWGG 3 cut(s) 593, 1328, 1825
PspEI GGTNACC 1 cut(s) 890
PspFI CCCAGC 1 cut(s) 1193
PspGI CCWGG 3 cut(s) 593, 1328, 1825
PspN4I GGNNCC 6 cut(s) 7, 87, 372, 653, 949, 2184
PspPI GGNCC 3 cut(s) 652, 2182, 2207
PspPPI RGGWCCY 1 cut(s) 652
PstNI CAGNNNCTG 1 cut(s) 1403
PsuI RGATCY 2 cut(s) 877, 1409
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 2 cut(s) 127, 2215
RsaNI GTAC 2 cut(s) 126, 2214
RseI CAYNNNNRTG 3 cut(s) 719, 976, 1452
SacII CCGCGG 1 cut(s) 389
SapI GCTCTTC 2 cut(s) 1161, 2034
SaqAI TTAA 9 cut(s) 180, 524, 731, 795, 858, 884, 1799, 2100, 2171
SatI GCNGC 6 cut(s) 143, 146, 165, 185, 212, 386
Sau96I GGNCC 3 cut(s) 652, 2182, 2207
SchI GAGTC 2 cut(s) 1880, 1985
ScrFI CCNGG 5 cut(s) 595, 1330, 1762, 1827, 2186
SduI GDGCHC 2 cut(s) 139, 429
SfaNI GCATC 4 cut(s) 223, 372, 1252, 1409
Sfr303I CCGCGG 1 cut(s) 389
SgrBI CCGCGG 1 cut(s) 389
SinI GGWCC 1 cut(s) 652
SmiMI CAYNNNNRTG 3 cut(s) 719, 976, 1452
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SseBI AGGCCT 1 cut(s) 1830
SspMI CTAG 2 cut(s) 1064, 1623
StuI AGGCCT 1 cut(s) 1830
StyD4I CCNGG 5 cut(s) 593, 1328, 1760, 1825, 2184
TaaI ACNGT 6 cut(s) 130, 604, 896, 988, 1522, 2218
TaiI ACGT 1 cut(s) 1921
TaqI TCGA 6 cut(s) 217, 571, 1002, 1358, 1440, 2166
TaqII GACCGA 1 cut(s) 45
TatI WGTACW 2 cut(s) 125, 2213
TauI GCSGC 4 cut(s) 148, 167, 187, 388
TfiI GAWTC 7 cut(s) 251, 394, 845, 955, 1322, 1457, 1724
Tru1I TTAA 9 cut(s) 180, 524, 731, 795, 858, 884, 1799, 2100, 2171
Tru9I TTAA 9 cut(s) 180, 524, 731, 795, 858, 884, 1799, 2100, 2171
TseFI GTSAC 2 cut(s) 890, 1550
TseI GCWGC 2 cut(s) 142, 211
Tsp45I GTSAC 2 cut(s) 890, 1550
TspGWI ACGGA 6 cut(s) 235, 303, 707, 1196, 1520, 1798
Vha464I CTTAAG 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 652
XapI RAATTY 3 cut(s) 72, 1007, 1541
XceI RCATGY 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 1029
XspI CTAG 2 cut(s) 1064, 1623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.