RchiOBHm_Chr7g0180231

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007850
Physical Location & Seq
Reverse (-)
2216771 .. 2222979
6209 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16070

Sequence Viewer

Length: 2220 bp
ATGTCTCTCGAGTCTTCTCCTCCGATCCCGTCCAACCGATCCTCGCCGGAACCCCAGCAACCACCACAACCCCCTCAAAACGACGACGTCGCGGCCACGACGACAACGACAACAGCCCCGGCCCCCACGCCGCCGCTGTCCCGCAGCAACCGCCCCTCCCGCTCTTGCACCCTACGCGCCGCGGCTAGGTTGCAGCAGCAGCAGCAGTACCAGCACGCCGAGCCGAGGAAGCCGAAGGCGGCGAGGAAGGAGCAGCCGCCGCAGCAGGAGAACGACGAGTCGTCGTCGCCGCAGCAATGCGGGAGCGCCAGCAAGATCGTGACGTCGCTGGTGGACCCGCCCTCGCAGTCGCAATTGCCGCGGTGGACGCTCCGGTCCATGTGGGAATTGGCCTCCGTGCTCAATTTTCTACATGCTTTTCGGCCGCTGTTGAATATACACGCCGAGTTCTCGGCTGAGGAATTGGAGACTGCTTTGATCACACCCAATGATAGCTTGAGTGACGTTCACATTCCCTTGTTGAAGGCGATTCCCCCTGTGACAAGAATGGCACTTACACGTGATACTTGGATAACTGTTTTATGCAGAAAGTTGAGAGACTGGTGGCATTGGGTTGCAGATGGAGATCTACCTATTGTTGCTTCACATGGGGCGGAGATTGAAGTATATAAAACACTTGATCCCGGTGTCCGTGTGGTCATTTTGAAAGCACTTTGTGATATTCGTGTTGAGCAAGAGGATATCCGGAACTATATTGACAATTCACTGAAACATGGTGTCCAGATTTCCACATTTCGTAAAGAACGCCTTGGGGGTGATTCACATGGAATCTCTTACTGGTATGAAGATGATCCAATTATTGGTTATCGGTTATATCGGGAGATAAGGAAGACTGAGGTGAAGAAAGTAAGAGCAAAGGGTTCCCATGTCCTTCCTAGTACAACATACCAGTGGGAAACAGTTGCAACCAATTTTGATGAATTTCAAGATGTTGGAGAAAAACTTTTCTCCAGTATCAATAGAACAGAGGCTGCTGTAGGAAAAAAGTTGAAAGGTGACATGCTTTTAGAGATTGAGAAGGTTCACAAGAGGAAAGAGAAGTTGCTGAAGAAGCAACACAGACAAGCTCTGCTCCTTGATAATTATGTGTCTGTGGATGGGCTTGGTCCAGGGCGCTCTCTTCGTGACAGAAAACCTGTCACATACACTTTTGATGATTATGACCGGTCAATCAATGAGGCCATCAAGATAACCAAAACGAAACAGCCATCCCCTGAGCCTTTACAGAAACGAGATGTTATGAAACATGAAGCTTCTAGCAATGGTAAATGGAGTGGTACTTCAAATTCCCATGAGCATGTGGGATTTAGCCCTCTGTCTCCTAAATCACCTGATTACGAAGACGAAGAAGATGATGTTGAGGAAGATGACAAATCTGAACAAATGGATAGAAGCAATCGGCGAAGACAGAGGCCCCAACGGTATTCTGCAAAAGAATTTGTTGAAGCAGTTTCAGATAATGATGCAGACTTTGACAGTGATGATGAAATAGTTGGTGAAGCTATTTATGATGAAGAATACTTAAAGAAACGTAAGGAGAGGAGAAAGTTTTCTAGTAGCTCTGAAGGAGATGAGGAGTATCGTTGGGAGGAAGAAAATCCAGAAGAGGAGGAAGAGGAGGAAGAAGATTCTGCTAGTGCCAGTGAGGAAAGTGATGAACCTCGAAAGTTTAAGAAACTGCGCGGTCGTCCTAAGGAATTGCCAGGACGTACTAGGAGGGAAGCTAAATTGAGGTCGGTTGATGAGCTCCAGTCAGGTCTAAGACGCAGTAAGAGGGCCACCAGAAATCGTATTGATTATCGGCAGTATGAGTTTTCAGATTCAGATACAGAGCCTATGAAACCTGAGAAGTCAAATGCATCAGATCGACACTCTGATGCAACTGAGAATGGTGACTATTCAATGGAAAGTCAAGATTCAGATGTTAATGATGGTCACCATGAAATGGAAGTTGGTCAGGCTGATCAGGCTGTTGAAAATTACCCAGAAACAGTAGAAAAAGAGGAAAACCAGCCACCTGAAAAATCAAATAGCCCAGGGGAAAATGAAGTTGGAGGTGTACAGAAAAGACGTTTCCTTGACCTAAACGAACTTGCCCCTGGTTCAGGTTTTGACGATGGTCCAAATACGATAATGAAAGATGATGCGGATGATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1

Protein Analysis

739

Amino Acids

84.34

Weight (kDa)

5.14

Isoelectric Point (pI)

64.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDT PF02791 132 - 177 8.6e-06 DDT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 373
AatII GACGTC 2 cut(s) 90, 326
AccB7I CCANNNNNTGG 2 cut(s) 860, 2005
AccBSI CCGCTC 1 cut(s) 162
AccII CGCG 5 cut(s) 92, 177, 182, 361, 1743
AccIII TCCGGA 1 cut(s) 744
AclWI GGATC 4 cut(s) 19, 33, 674, 845
AcoI YGGCCR 2 cut(s) 93, 422
AcsI RAATTY 3 cut(s) 980, 1345, 1496
AcuI CTGAAG 2 cut(s) 1127, 1644
AcvI CACGTG 1 cut(s) 560
AcyI GRCGYC 2 cut(s) 87, 323
AdeI CACNNNGTG 1 cut(s) 716
AfaI GTAC 5 cut(s) 209, 940, 1339, 1771, 2121
AfiI CCNNNNNNNGG 5 cut(s) 186, 225, 860, 2005, 2165
AflIII ACRYGT 1 cut(s) 557
AgeI ACCGGT 1 cut(s) 1224
AhdI GACNNNNNGTC 1 cut(s) 280
AjnI CCWGG 4 cut(s) 1168, 1762, 2095, 2158
AjuI GAANNNNNNNTTGG 4 cut(s) 1995, 2027, 2094, 2126
AluBI AGCT 7 cut(s) 495, 1127, 1313, 1562, 1620, 1784, 1807
AluI AGCT 7 cut(s) 495, 1127, 1313, 1562, 1620, 1784, 1807
Alw21I GWGCWC 2 cut(s) 402, 1809
Alw26I GTCTC 4 cut(s) 9, 461, 591, 1383
AlwI GGATC 4 cut(s) 19, 33, 674, 845
AlwNI CAGNNNCTG 1 cut(s) 1031
Ama87I CYCGRG 1 cut(s) 8
Aor13HI TCCGGA 1 cut(s) 744
AoxI GGCC 7 cut(s) 93, 120, 390, 422, 1239, 1472, 1836
ApeKI GCWGC 9 cut(s) 144, 193, 196, 199, 202, 253, 262, 292, 1031
ApoI RAATTY 3 cut(s) 980, 1345, 1496
AsiGI ACCGGT 1 cut(s) 1224
Asp700I GAANNNNTTC 1 cut(s) 1609
AspLEI GCGC 4 cut(s) 179, 308, 1176, 1743
AspS9I GGNCC 7 cut(s) 121, 334, 375, 1166, 1473, 1836, 2180
AsuC2I CCSGG 2 cut(s) 119, 684
AsuHPI GGTGA 7 cut(s) 827, 910, 1067, 1380, 1568, 1964, 1988
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 4 cut(s) 334, 375, 1166, 2180
AxyI CCTNAGG 1 cut(s) 1752
BanII GRGCYC 1 cut(s) 1809
BbrPI CACGTG 1 cut(s) 560
BbsI GAAGAC 4 cut(s) 6, 896, 1407, 1471
Bbv12I GWGCWC 2 cut(s) 402, 1809
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 9 cut(s) 156, 205, 208, 211, 214, 265, 274, 304, 1018
BccI CCATC 6 cut(s) 614, 1151, 1250, 1276, 1985, 2171
BciT130I CCWGG 4 cut(s) 1170, 1764, 2097, 2160
BclI TGATCA 2 cut(s) 477, 2023
BcnI CCSGG 2 cut(s) 119, 684
BcoDI GTCTC 4 cut(s) 9, 461, 591, 1383
BfaI CTAG 6 cut(s) 186, 936, 1317, 1614, 1695, 1773
BfmI CTRYAG 1 cut(s) 1035
BfoI RGCGCY 2 cut(s) 309, 1177
BglII AGATCT 1 cut(s) 625
Bme1390I CCNGG 6 cut(s) 119, 684, 1170, 1764, 2097, 2160
Bme18I GGWCC 4 cut(s) 334, 375, 1166, 2180
BmeRI GACNNNNNGTC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 7 cut(s) 121, 334, 375, 1166, 1473, 1836, 2180
BmiI GGNNCC 5 cut(s) 51, 123, 336, 922, 1475
BmrFI CCNGG 6 cut(s) 119, 684, 1170, 1764, 2097, 2160
BmsI GCATC 4 cut(s) 1513, 1927, 1928, 2194
BoxI GACNNNNGTC 1 cut(s) 2178
BpiI GAAGAC 4 cut(s) 6, 896, 1407, 1471
BpmI CTGGAG 2 cut(s) 994, 1793
Bpu10I CCTNAGC 2 cut(s) 456, 1275
BpuEI CTTGAG 1 cut(s) 517
BpuMI CCSGG 2 cut(s) 119, 684
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 624
BsaHI GRCGYC 2 cut(s) 87, 323
BsaJI CCNNGG 9 cut(s) 117, 180, 224, 359, 808, 1169, 2095, 2096, 2158
BsaWI WCCGGW 3 cut(s) 372, 744, 1224
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 5 cut(s) 186, 225, 860, 2005, 2165
Bse118I RCCGGY 1 cut(s) 1224
Bse1I ACTGG 6 cut(s) 605, 842, 949, 1011, 1701, 1810
Bse21I CCTNAGG 1 cut(s) 1752
Bse3DI GCAATG 2 cut(s) 302, 1327
Bse8I GATNNNNATC 1 cut(s) 624
BseAI TCCGGA 1 cut(s) 744
BseBI CCWGG 4 cut(s) 1170, 1764, 2097, 2160
BseDI CCNNGG 9 cut(s) 117, 180, 224, 359, 808, 1169, 2095, 2096, 2158
BseGI GGATG 3 cut(s) 1162, 1268, 2215
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 5 cut(s) 186, 225, 860, 2005, 2165
BseMI GCAATG 2 cut(s) 302, 1327
BseMII CTCAG 5 cut(s) 447, 885, 1266, 1896, 1935
BseNI ACTGG 6 cut(s) 605, 842, 949, 1011, 1701, 1810
BseRI GAGGAG 5 cut(s) 9, 1615, 1649, 1682, 1691
BseX3I CGGCCG 1 cut(s) 422
BseXI GCAGC 9 cut(s) 156, 205, 208, 211, 214, 265, 274, 304, 1018
BseYI CCCAGC 1 cut(s) 54
Bsh1236I CGCG 5 cut(s) 92, 177, 182, 361, 1743
Bsh1285I CGRYCG 2 cut(s) 425, 1747
BshFI GGCC 7 cut(s) 95, 122, 392, 424, 1241, 1474, 1838
BshTI ACCGGT 1 cut(s) 1224
BsiEI CGRYCG 2 cut(s) 425, 1747
BsiHKAI GWGCWC 2 cut(s) 402, 1809
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 6 cut(s) 47, 119, 373, 684, 745, 1225
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 5 cut(s) 186, 225, 860, 2005, 2165
BsmAI GTCTC 4 cut(s) 9, 461, 591, 1383
BsmFI GGGAC 1 cut(s) 124
BsnI GGCC 7 cut(s) 95, 122, 392, 424, 1241, 1474, 1838
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 402, 1809
Bsp13I TCCGGA 1 cut(s) 744
Bsp1407I TGTACA 1 cut(s) 2119
Bsp143I GATC 9 cut(s) 24, 38, 315, 477, 625, 679, 850, 1924, 2023
BspANI GGCC 7 cut(s) 95, 122, 392, 424, 1241, 1474, 1838
BspCNI CTCAG 5 cut(s) 448, 886, 1267, 1897, 1936
BspEI TCCGGA 1 cut(s) 744
BspFNI CGCG 5 cut(s) 92, 177, 182, 361, 1743
BspLI GGNNCC 5 cut(s) 51, 123, 336, 922, 1475
BspPI GGATC 4 cut(s) 19, 33, 674, 845
BsrBI CCGCTC 1 cut(s) 162
BsrDI GCAATG 2 cut(s) 302, 1327
BsrFI RCCGGY 1 cut(s) 1224
BsrGI TGTACA 1 cut(s) 2119
BsrI ACTGG 6 cut(s) 605, 842, 949, 1011, 1701, 1810
BssAI RCCGGY 1 cut(s) 1224
BssECI CCNNGG 9 cut(s) 117, 180, 224, 359, 808, 1169, 2095, 2096, 2158
BssMI GATC 9 cut(s) 24, 38, 315, 477, 625, 679, 850, 1924, 2023
BssNI GRCGYC 2 cut(s) 87, 323
BssT1I CCWWGG 1 cut(s) 808
Bst2UI CCWGG 4 cut(s) 1170, 1764, 2097, 2160
Bst4CI ACNGT 5 cut(s) 577, 961, 1482, 1538, 2053
Bst6I CTCTTC 3 cut(s) 1185, 1659, 1668
BstACI GRCGYC 2 cut(s) 87, 323
BstAUI TGTACA 1 cut(s) 2119
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 2 cut(s) 216, 310
BstDEI CTNAG 7 cut(s) 456, 894, 1275, 1752, 1820, 1905, 1944
BstDSI CCRYGG 2 cut(s) 180, 359
BstEII GGTNACC 1 cut(s) 1994
BstENI CCTNNNNNAGG 1 cut(s) 2163
BstF5I GGATG 3 cut(s) 1162, 1268, 2215
BstFNI CGCG 5 cut(s) 92, 177, 182, 361, 1743
BstH2I RGCGCY 2 cut(s) 309, 1177
BstHHI GCGC 4 cut(s) 179, 308, 1176, 1743
BstKTI GATC 9 cut(s) 27, 41, 318, 480, 628, 682, 853, 1927, 2026
BstMAI GTCTC 4 cut(s) 9, 461, 591, 1383
BstMBI GATC 9 cut(s) 24, 38, 315, 477, 625, 679, 850, 1924, 2023
BstMCI CGRYCG 2 cut(s) 425, 1747
BstNI CCWGG 4 cut(s) 1170, 1764, 2097, 2160
BstNSI RCATGY 3 cut(s) 416, 1063, 1361
BstPAI GACNNNNGTC 1 cut(s) 2178
BstPI GGTNACC 1 cut(s) 1994
BstSCI CCNGG 6 cut(s) 117, 682, 1168, 1762, 2095, 2158
BstSFI CTRYAG 1 cut(s) 1035
BstUI CGCG 5 cut(s) 92, 177, 182, 361, 1743
BstV1I GCAGC 9 cut(s) 156, 205, 208, 211, 214, 265, 274, 304, 1018
BstV2I GAAGAC 4 cut(s) 6, 896, 1407, 1471
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BstZI CGGCCG 1 cut(s) 422
Bsu36I CCTNAGG 1 cut(s) 1752
BsuRI GGCC 7 cut(s) 95, 122, 392, 424, 1241, 1474, 1838
BtgI CCRYGG 2 cut(s) 180, 359
BtsCI GGATG 3 cut(s) 1162, 1268, 2215
BtsIMutI CAGTG 4 cut(s) 764, 956, 1543, 1708
Cac8I GCNNGC 2 cut(s) 216, 310
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 4 cut(s) 179, 308, 1176, 1743
Cfr10I RCCGGY 1 cut(s) 1224
Cfr13I GGNCC 7 cut(s) 121, 334, 375, 1166, 1473, 1836, 2180
Cfr42I CCGCGG 2 cut(s) 183, 362
CseI GACGC 2 cut(s) 376, 1833
Csp6I GTAC 5 cut(s) 208, 939, 1338, 1770, 2120
CspAI ACCGGT 1 cut(s) 1224
CviQI GTAC 5 cut(s) 208, 939, 1338, 1770, 2120
DdeI CTNAG 7 cut(s) 456, 894, 1275, 1752, 1820, 1905, 1944
DpnI GATC 9 cut(s) 26, 40, 317, 479, 627, 681, 852, 1926, 2025
DpnII GATC 9 cut(s) 24, 38, 315, 477, 625, 679, 850, 1924, 2023
DraIII CACNNNGTG 1 cut(s) 716
DrdI GACNNNNNNGTC 1 cut(s) 373
DriI GACNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 373
EaeI YGGCCR 2 cut(s) 93, 422
EagI CGGCCG 1 cut(s) 422
Eam1104I CTCTTC 3 cut(s) 1185, 1659, 1668
Eam1105I GACNNNNNGTC 1 cut(s) 280
EarI CTCTTC 3 cut(s) 1185, 1659, 1668
EciI GGCGGA 1 cut(s) 668
Ecl136II GAGCTC 1 cut(s) 1807
EclXI CGGCCG 1 cut(s) 422
Eco130I CCWWGG 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 1809
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 4 cut(s) 334, 375, 1166, 2180
Eco52I CGGCCG 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 1807
Eco57I CTGAAG 2 cut(s) 1127, 1644
Eco72I CACGTG 1 cut(s) 560
Eco81I CCTNAGG 1 cut(s) 1752
Eco88I CYCGRG 1 cut(s) 8
Eco91I GGTNACC 1 cut(s) 1994
EcoICRI GAGCTC 1 cut(s) 1807
EcoNI CCTNNNNNAGG 1 cut(s) 2163
EcoO109I RGGNCCY 1 cut(s) 1473
EcoO65I GGTNACC 1 cut(s) 1994
EcoRII CCWGG 4 cut(s) 1168, 1762, 2095, 2158
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 808
EcoT22I ATGCAT 1 cut(s) 1921
EcoT38I GRGCYC 1 cut(s) 1809
ErhI CCWWGG 1 cut(s) 808
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 1 cut(s) 124
FauI CCCGC 4 cut(s) 149, 167, 293, 345
FbaI TGATCA 2 cut(s) 477, 2023
FokI GGATG 2 cut(s) 1169, 1255
FriOI GRGCYC 1 cut(s) 1809
FspBI CTAG 6 cut(s) 186, 936, 1317, 1614, 1695, 1773
GlaI GCGC 4 cut(s) 178, 307, 1175, 1742
GsaI CCCAGC 1 cut(s) 58
GsuI CTGGAG 2 cut(s) 994, 1793
HaeII RGCGCY 2 cut(s) 309, 1177
HaeIII GGCC 7 cut(s) 95, 122, 392, 424, 1241, 1474, 1838
HapII CCGG 6 cut(s) 47, 119, 373, 684, 745, 1225
HgaI GACGC 2 cut(s) 376, 1833
HhaI GCGC 4 cut(s) 179, 308, 1176, 1743
Hin1I GRCGYC 2 cut(s) 87, 323
Hin6I GCGC 4 cut(s) 177, 306, 1174, 1741
HinP1I GCGC 4 cut(s) 177, 306, 1174, 1741
HindIII AAGCTT 1 cut(s) 1311
HinfI GANTC 8 cut(s) 11, 278, 529, 818, 828, 1688, 1880, 1976
HpaII CCGG 6 cut(s) 47, 119, 373, 684, 745, 1225
HphI GGTGA 7 cut(s) 827, 910, 1067, 1380, 1568, 1964, 1988
Hpy166II GTNNAC 5 cut(s) 334, 366, 508, 1084, 2120
Hpy188I TCNGA 9 cut(s) 24, 1438, 1516, 1624, 1879, 1885, 1924, 1936, 1981
Hpy8I GTNNAC 5 cut(s) 334, 366, 508, 1084, 2120
Hpy99I CGWCG 8 cut(s) 86, 89, 92, 103, 278, 286, 289, 328
HpyAV CCTTC 6 cut(s) 229, 241, 517, 941, 1072, 1619
HpyCH4III ACNGT 5 cut(s) 577, 961, 1482, 1538, 2053
HpyCH4IV ACGT 7 cut(s) 87, 323, 504, 559, 1591, 1768, 2131
HpyCH4V TGCA 9 cut(s) 168, 193, 585, 617, 965, 1490, 1526, 1919, 1940
HpyF3I CTNAG 7 cut(s) 456, 894, 1275, 1752, 1820, 1905, 1944
HpySE526I ACGT 7 cut(s) 87, 323, 504, 559, 1591, 1768, 2131
Hsp92I GRCGYC 2 cut(s) 87, 323
HspAI GCGC 4 cut(s) 177, 306, 1174, 1741
Kpn2I TCCGGA 1 cut(s) 744
Ksp22I TGATCA 2 cut(s) 477, 2023
KspI CCGCGG 2 cut(s) 183, 362
Kzo9I GATC 9 cut(s) 24, 38, 315, 477, 625, 679, 850, 1924, 2023
LmnI GCTCC 5 cut(s) 250, 303, 375, 1137, 1812
Lsp1109I GCAGC 9 cut(s) 156, 205, 208, 211, 214, 265, 274, 304, 1018
LweI GCATC 4 cut(s) 1513, 1927, 1928, 2194
MaeI CTAG 6 cut(s) 186, 936, 1317, 1614, 1695, 1773
MaeII ACGT 7 cut(s) 87, 323, 504, 559, 1591, 1768, 2131
MaeIII GTNAC 8 cut(s) 319, 500, 538, 1055, 1184, 1198, 1952, 1994
MalI GATC 9 cut(s) 26, 40, 317, 479, 627, 681, 852, 1926, 2025
MbiI CCGCTC 1 cut(s) 162
MboI GATC 9 cut(s) 24, 38, 315, 477, 625, 679, 850, 1924, 2023
MfeI CAATTG 1 cut(s) 353
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 2 cut(s) 402, 1809
MlyI GAGTC 2 cut(s) 20, 287
MmeI TCCRAC 3 cut(s) 57, 973, 2092
Mph1103I ATGCAT 1 cut(s) 1921
MroI TCCGGA 1 cut(s) 744
MroXI GAANNNNTTC 1 cut(s) 1609
MseI TTAA 3 cut(s) 1583, 1731, 1986
MslI CAYNNNNRTG 3 cut(s) 949, 1356, 1935
MspA1I CMGCKG 4 cut(s) 136, 182, 361, 427
MspI CCGG 6 cut(s) 47, 119, 373, 684, 745, 1225
MspR9I CCNGG 6 cut(s) 119, 684, 1170, 1764, 2097, 2160
MunI CAATTG 1 cut(s) 353
MvaI CCWGG 4 cut(s) 1170, 1764, 2097, 2160
MvnI CGCG 5 cut(s) 92, 177, 182, 361, 1743
NciI CCSGG 2 cut(s) 119, 684
NdeII GATC 9 cut(s) 24, 38, 315, 477, 625, 679, 850, 1924, 2023
NlaIV GGNNCC 5 cut(s) 51, 123, 336, 922, 1475
NmeAIII GCCGAG 4 cut(s) 244, 249, 431, 469
NmuCI GTSAC 8 cut(s) 319, 500, 538, 1055, 1184, 1198, 1952, 1994
NsiI ATGCAT 1 cut(s) 1921
NspI RCATGY 3 cut(s) 416, 1063, 1361
PaeR7I CTCGAG 1 cut(s) 8
PasI CCCWGGG 1 cut(s) 2096
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 1609
PfeI GAWTC 6 cut(s) 529, 818, 828, 1688, 1880, 1976
PflFI GACNNNGTC 1 cut(s) 86
PflMI CCANNNNNTGG 2 cut(s) 860, 2005
PinAI ACCGGT 1 cut(s) 1224
PleI GAGTC 2 cut(s) 19, 286
PmaCI CACGTG 1 cut(s) 560
PmlI CACGTG 1 cut(s) 560
PpsI GAGTC 2 cut(s) 19, 286
Ppu21I YACGTR 1 cut(s) 560
PshAI GACNNNNGTC 1 cut(s) 2178
Psp124BI GAGCTC 1 cut(s) 1809
Psp6I CCWGG 4 cut(s) 1168, 1762, 2095, 2158
PspCI CACGTG 1 cut(s) 560
PspEI GGTNACC 1 cut(s) 1994
PspFI CCCAGC 1 cut(s) 54
PspGI CCWGG 4 cut(s) 1168, 1762, 2095, 2158
PspN4I GGNNCC 5 cut(s) 51, 123, 336, 922, 1475
PspPI GGNCC 7 cut(s) 121, 334, 375, 1166, 1473, 1836, 2180
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 1 cut(s) 625
PsyI GACNNNGTC 1 cut(s) 86
RsaI GTAC 5 cut(s) 209, 940, 1339, 1771, 2121
RsaNI GTAC 5 cut(s) 208, 939, 1338, 1770, 2120
RseI CAYNNNNRTG 3 cut(s) 949, 1356, 1935
SacI GAGCTC 1 cut(s) 1809
SacII CCGCGG 2 cut(s) 183, 362
SaqAI TTAA 3 cut(s) 1583, 1731, 1986
Sau3AI GATC 9 cut(s) 24, 38, 315, 477, 625, 679, 850, 1924, 2023
Sau96I GGNCC 7 cut(s) 121, 334, 375, 1166, 1473, 1836, 2180
SchI GAGTC 2 cut(s) 20, 287
ScrFI CCNGG 6 cut(s) 119, 684, 1170, 1764, 2097, 2160
SduI GDGCHC 2 cut(s) 402, 1809
SfaNI GCATC 4 cut(s) 1513, 1927, 1928, 2194
SfcI CTRYAG 1 cut(s) 1035
Sfr274I CTCGAG 1 cut(s) 8
Sfr303I CCGCGG 2 cut(s) 183, 362
SgrBI CCGCGG 2 cut(s) 183, 362
SinI GGWCC 4 cut(s) 334, 375, 1166, 2180
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 3 cut(s) 949, 1356, 1935
SmlI CTYRAG 2 cut(s) 8, 496
SmoI CTYRAG 2 cut(s) 8, 496
SspMI CTAG 6 cut(s) 186, 936, 1317, 1614, 1695, 1773
SstI GAGCTC 1 cut(s) 1809
StyD4I CCNGG 6 cut(s) 117, 682, 1168, 1762, 2095, 2158
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 5 cut(s) 577, 961, 1482, 1538, 2053
TaiI ACGT 7 cut(s) 90, 326, 507, 562, 1594, 1771, 2134
TaqI TCGA 3 cut(s) 9, 1723, 1927
TatI WGTACW 2 cut(s) 938, 2119
TfiI GAWTC 6 cut(s) 529, 818, 828, 1688, 1880, 1976
Tru1I TTAA 3 cut(s) 1583, 1731, 1986
Tru9I TTAA 3 cut(s) 1583, 1731, 1986
TscAI CASTG 4 cut(s) 771, 956, 1543, 1708
TseFI GTSAC 8 cut(s) 319, 500, 538, 1055, 1184, 1198, 1952, 1994
TseI GCWGC 9 cut(s) 144, 193, 196, 199, 202, 253, 262, 292, 1031
Tsp45I GTSAC 8 cut(s) 319, 500, 538, 1055, 1184, 1198, 1952, 1994
TspGWI ACGGA 2 cut(s) 385, 680
TspRI CASTG 4 cut(s) 771, 956, 1543, 1708
Tth111I GACNNNGTC 1 cut(s) 86
Van91I CCANNNNNTGG 2 cut(s) 860, 2005
VpaK11BI GGWCC 4 cut(s) 334, 375, 1166, 2180
XagI CCTNNNNNAGG 1 cut(s) 2163
XapI RAATTY 3 cut(s) 980, 1345, 1496
XceI RCATGY 3 cut(s) 416, 1063, 1361
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XhoI CTCGAG 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 1609
XspI CTAG 6 cut(s) 186, 936, 1317, 1614, 1695, 1773
ZraI GACGTC 2 cut(s) 88, 324
Zsp2I ATGCAT 1 cut(s) 1921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.