AT1G20870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008559
Physical Location & Seq
Reverse (-)
7259023 .. 7261524
2502 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20870.1

Sequence Viewer

Length: 1392 bp
ATGAGTCTGTATAGCGACGGATTATCGAGTGATCAGTATTTCTTGGTTAATTTCATCATGAGCAACTACTTGGGTCCTGATGTCTATTCTGATAACCCAAGATGTTCATCTTCTCAAAGATTAGCTAGAGGTTTGCCACCTTACACTTTGATGCACATTGGATCTTCCTCTCTAACTGTTTCCCAGCTACAGAACTTGTACTATAATGTACTCAGAAACGCCAAGTCCAGCCTCCTTCTTCATCCAGACATGATTTATATGTATCTCAAGGGATATCTACCATTGGAACCAAGTGGAAAGTTTCCTCAGTTCACGCATTTTTTCCCGACGAATCTTCACCCGCAGAAAAGGTATTCCCCGAGCCACGAGATTGTGAAAGGAATTGTTGTTATTGATGATCCTGCAGTAGGGTTCATAAACAAGGAAGAACTCCAAAGGTTCAGATGCTTGTCGCGTTTGGACGACCTGAAAATCGATAGAGTCACGTCTTTATCTCCTCGGGTTAATCTGGATGAAAGCCGGGAAACTGAGCAGGACTGTTCAAGAAATGGTGATGCGACAGCTAATGGAGTTGTCACTAACGAGGACTACAATTCATCTGGTGAGCTTCAAGAAACGTGCAAGAGAAAGGAAGGGGAAGACGCGGTTGCTAGTTGCGTAATCAGTGAACCAGAAAGACTTTCGGGAGACATACCAGAAAGCCAGGGAATGAAGCAGGACTGTTCAAGAAACGGAGAATCTGCATTCAGTGGAATTGTCTCTGACCAAGACTACTATTCATTTGTAAAGCTTCCAGAAACATGCAAGCGAAAGAACAAGGAAGAAGAAGCAGTTACTGGTCATGCTGTTAGTGGAACGAGTAAAACCCCTGAAAGATTTCGTGAGACATACAAGAGAAGACGGTTCAAGAACTCATCAAAAAAGGCAACAAACAAGAATGGTGAAACTCTTATGGAAAGAGAAAAAACAGATAAGCCTATACCTTTTTCTTCGGAGATGAAGGAAAGCGATGCAGAACCGTCGGTTGTAACTACTGGAACAGCCAGTAAAGAAACTCTAGGATCATCTGTTGGTGTTGTTGACATTGGTGTCAACAAGGTTGCTTACTTTTTTCAGGTTGCTTTGCCCGGTGTCCGCAAAGATTACGGTGAATTCAGCTGTGAGATTGAATCAGATGGAAAGGTTATACTGGAGGGATCAACTACAAGAGGCGAAAAAAATATCAAGAGACATTCTCGGGTGTTCGAGATGAATATCCGGAAGCTGTGTCCGCCTGGACCATTCAAGCTGTGCTTTAACCTCCCGGGACCAGTTGATCCGCGGCTATTCTCTCCTAACTTCCGATCAGATGGTATCTTCGAGGGAGTCATCATCCGACACAAAAACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009893 GO:0010604 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0060255 GO:0065007 GO:0080090 GO:1901535 GO:1901537
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

463

Amino Acids

51.93

Weight (kDa)

7.07

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1088
AccII CGCG 3 cut(s) 454, 644, 1321
AccIII TCCGGA 1 cut(s) 1257
AciI CCGC 6 cut(s) 341, 644, 1135, 1271, 1319, 1321
AclWI GGATC 5 cut(s) 169, 392, 1069, 1204, 1310
AcsI RAATTY 1 cut(s) 1151
AfaI GTAC 2 cut(s) 200, 210
AfiI CCNNNNNNNGG 1 cut(s) 407
AgsI TTSAA 6 cut(s) 543, 611, 726, 907, 1169, 1285
AjiI CACGTC 1 cut(s) 486
AjnI CCWGG 2 cut(s) 702, 1273
AluBI AGCT 8 cut(s) 125, 187, 563, 607, 790, 1158, 1264, 1288
AluI AGCT 8 cut(s) 125, 187, 563, 607, 790, 1158, 1264, 1288
Alw26I GTCTC 4 cut(s) 681, 763, 878, 1222
AlwI GGATC 5 cut(s) 169, 392, 1069, 1204, 1310
AlwNI CAGNNNCTG 1 cut(s) 836
Ama87I CYCGRG 4 cut(s) 358, 498, 1236, 1303
Aor13HI TCCGGA 1 cut(s) 1257
ApoI RAATTY 1 cut(s) 1151
Asp700I GAANNNNTTC 1 cut(s) 876
AspS9I GGNCC 3 cut(s) 74, 1277, 1307
AsuC2I CCSGG 4 cut(s) 521, 1128, 1304, 1305
AsuHPI GGTGA 5 cut(s) 329, 563, 614, 953, 1160
AvaI CYCGRG 4 cut(s) 358, 498, 1236, 1303
AvaII GGWCC 3 cut(s) 74, 1277, 1307
BauI CACGAG 1 cut(s) 365
BbsI GAAGAC 2 cut(s) 645, 904
BccI CCATC 2 cut(s) 1169, 1343
BcgI CGANNNNNNTGC 2 cut(s) 1002, 1036
BciT130I CCWGG 2 cut(s) 704, 1275
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 4 cut(s) 521, 1128, 1304, 1305
BcoDI GTCTC 4 cut(s) 681, 763, 878, 1222
BfaI CTAG 3 cut(s) 126, 651, 1058
BfmI CTRYAG 2 cut(s) 188, 402
BisI GCNGC 1 cut(s) 1322
BlsI GCNGC 1 cut(s) 1323
Bme1390I CCNGG 6 cut(s) 521, 704, 1128, 1275, 1304, 1305
Bme18I GGWCC 3 cut(s) 74, 1277, 1307
BmeT110I CYCGRG 4 cut(s) 358, 498, 1236, 1303
BmgBI CACGTC 1 cut(s) 486
BmgT120I GGNCC 3 cut(s) 74, 1277, 1307
BmiI GGNNCC 3 cut(s) 75, 288, 1308
BmrFI CCNGG 6 cut(s) 521, 704, 1128, 1275, 1304, 1305
BmsI GCATC 4 cut(s) 141, 434, 544, 1000
BpiI GAAGAC 2 cut(s) 645, 904
BplI GAGNNNNNCTC 2 cut(s) 1219, 1251
BpmI CTGGAG 1 cut(s) 1211
BpuEI CTTGAG 1 cut(s) 251
BpuMI CCSGG 4 cut(s) 521, 1128, 1304, 1305
Bsa29I ATCGAT 1 cut(s) 474
BsaBI GATNNNNATC 2 cut(s) 106, 1253
BsaJI CCNNGG 4 cut(s) 497, 703, 1303, 1319
BsaWI WCCGGW 1 cut(s) 1257
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse1I ACTGG 5 cut(s) 841, 1039, 1044, 1194, 1310
Bse8I GATNNNNATC 2 cut(s) 106, 1253
BseAI TCCGGA 1 cut(s) 1257
BseBI CCWGG 2 cut(s) 704, 1275
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 4 cut(s) 497, 703, 1303, 1319
BseGI GGATG 3 cut(s) 241, 517, 1371
BseJI GATNNNNATC 2 cut(s) 106, 1253
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMII CTCAG 3 cut(s) 226, 320, 519
BseNI ACTGG 5 cut(s) 841, 1039, 1044, 1194, 1310
BseRI GAGGAG 1 cut(s) 486
BseYI CCCAGC 1 cut(s) 183
Bsh1236I CGCG 3 cut(s) 454, 644, 1321
BshVI ATCGAT 1 cut(s) 474
BsiHKCI CYCGRG 4 cut(s) 358, 498, 1236, 1303
BsiSI CCGG 4 cut(s) 520, 1128, 1258, 1304
BslFI GGGAC 1 cut(s) 1320
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 4 cut(s) 681, 763, 878, 1222
BsmFI GGGAC 1 cut(s) 1320
BsmI GAATGC 1 cut(s) 743
BsoBI CYCGRG 4 cut(s) 358, 498, 1236, 1303
Bsp13I TCCGGA 1 cut(s) 1257
Bsp143I GATC 7 cut(s) 31, 161, 397, 1061, 1196, 1315, 1343
BspACI CCGC 6 cut(s) 341, 644, 1135, 1271, 1319, 1321
BspCNI CTCAG 3 cut(s) 225, 319, 520
BspDI ATCGAT 1 cut(s) 474
BspEI TCCGGA 1 cut(s) 1257
BspFNI CGCG 3 cut(s) 454, 644, 1321
BspHI TCATGA 1 cut(s) 57
BspLI GGNNCC 3 cut(s) 75, 288, 1308
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 5 cut(s) 169, 392, 1069, 1204, 1310
BsrI ACTGG 5 cut(s) 841, 1039, 1044, 1194, 1310
BssECI CCNNGG 4 cut(s) 497, 703, 1303, 1319
BssMI GATC 7 cut(s) 31, 161, 397, 1061, 1196, 1315, 1343
BssSI CACGAG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 2 cut(s) 704, 1275
Bst4CI ACNGT 6 cut(s) 178, 539, 722, 903, 1020, 1148
BstC8I GCNNGC 1 cut(s) 806
BstDEI CTNAG 3 cut(s) 212, 306, 528
BstDSI CCRYGG 1 cut(s) 1319
BstENI CCTNNNNNAGG 1 cut(s) 405
BstF5I GGATG 3 cut(s) 241, 517, 1371
BstFNI CGCG 3 cut(s) 454, 644, 1321
BstKTI GATC 7 cut(s) 34, 164, 400, 1064, 1199, 1318, 1346
BstMAI GTCTC 4 cut(s) 681, 763, 878, 1222
BstMBI GATC 7 cut(s) 31, 161, 397, 1061, 1196, 1315, 1343
BstMWI GCNNNNNNNGC 1 cut(s) 1270
BstNI CCWGG 2 cut(s) 704, 1275
BstNSI RCATGY 1 cut(s) 804
BstSCI CCNGG 6 cut(s) 519, 702, 1126, 1273, 1302, 1303
BstSFI CTRYAG 2 cut(s) 188, 402
BstUI CGCG 3 cut(s) 454, 644, 1321
BstV2I GAAGAC 2 cut(s) 645, 904
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
Bsu15I ATCGAT 1 cut(s) 474
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 1319
BtgZI GCGATG 1 cut(s) 1023
BtrI CACGTC 1 cut(s) 486
BtsCI GGATG 3 cut(s) 241, 517, 1371
BtsIMutI CAGTG 2 cut(s) 670, 754
Cac8I GCNNGC 1 cut(s) 806
CaiI CAGNNNCTG 1 cut(s) 836
CciI TCATGA 1 cut(s) 57
Cfr13I GGNCC 3 cut(s) 74, 1277, 1307
Cfr42I CCGCGG 1 cut(s) 1322
Cfr9I CCCGGG 1 cut(s) 1303
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 1 cut(s) 650
Csp6I GTAC 2 cut(s) 199, 209
CviAII CATG 4 cut(s) 58, 250, 801, 842
CviQI GTAC 2 cut(s) 199, 209
DdeI CTNAG 3 cut(s) 212, 306, 528
DpnI GATC 7 cut(s) 33, 163, 399, 1063, 1198, 1317, 1345
DpnII GATC 7 cut(s) 31, 161, 397, 1061, 1196, 1315, 1343
DrdI GACNNNNNNGTC 1 cut(s) 1088
DseDI GACNNNNNNGTC 1 cut(s) 1088
EciI GGCGGA 1 cut(s) 1260
Eco32I GATATC 1 cut(s) 275
Eco47I GGWCC 3 cut(s) 74, 1277, 1307
Eco88I CYCGRG 4 cut(s) 358, 498, 1236, 1303
EcoNI CCTNNNNNAGG 1 cut(s) 405
EcoO109I RGGNCCY 1 cut(s) 74
EcoRI GAATTC 1 cut(s) 1151
EcoRII CCWGG 2 cut(s) 702, 1273
EcoRV GATATC 1 cut(s) 275
FaeI CATG 4 cut(s) 61, 253, 804, 845
FalI AAGNNNNNCTT 2 cut(s) 1277, 1309
FaqI GGGAC 1 cut(s) 1320
FatI CATG 4 cut(s) 57, 249, 800, 841
FauI CCCGC 1 cut(s) 348
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 1 cut(s) 1322
FokI GGATG 3 cut(s) 228, 524, 1358
Fsp4HI GCNGC 1 cut(s) 1322
FspBI CTAG 3 cut(s) 126, 651, 1058
GluI GCNGC 1 cut(s) 1322
GsaI CCCAGC 1 cut(s) 187
GsuI CTGGAG 1 cut(s) 1211
HapII CCGG 4 cut(s) 520, 1128, 1258, 1304
HgaI GACGC 1 cut(s) 650
Hin1II CATG 4 cut(s) 61, 253, 804, 845
HincII GTYRAC 2 cut(s) 1081, 1093
HindII GTYRAC 2 cut(s) 1081, 1093
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 6 cut(s) 4, 331, 480, 737, 1169, 1365
HpaII CCGG 4 cut(s) 520, 1128, 1258, 1304
HphI GGTGA 5 cut(s) 329, 563, 614, 953, 1160
Hpy166II GTNNAC 4 cut(s) 312, 668, 1081, 1093
Hpy188I TCNGA 9 cut(s) 91, 215, 443, 763, 994, 1174, 1343, 1348, 1376
Hpy8I GTNNAC 4 cut(s) 312, 668, 1081, 1093
Hpy99I CGWCG 3 cut(s) 20, 331, 1024
HpyAV CCTTC 3 cut(s) 245, 626, 994
HpyCH4III ACNGT 6 cut(s) 178, 539, 722, 903, 1020, 1148
HpyCH4IV ACGT 2 cut(s) 485, 617
HpyCH4V TGCA 6 cut(s) 154, 404, 621, 743, 804, 1013
HpyF10VI GCNNNNNNNGC 1 cut(s) 1270
HpyF3I CTNAG 3 cut(s) 212, 306, 528
HpySE526I ACGT 2 cut(s) 485, 617
Hsp92II CATG 4 cut(s) 61, 253, 804, 845
Kpn2I TCCGGA 1 cut(s) 1257
Ksp22I TGATCA 1 cut(s) 31
KspI CCGCGG 1 cut(s) 1322
Kzo9I GATC 7 cut(s) 31, 161, 397, 1061, 1196, 1315, 1343
LweI GCATC 4 cut(s) 141, 434, 544, 1000
MaeI CTAG 3 cut(s) 126, 651, 1058
MaeII ACGT 2 cut(s) 485, 617
MaeIII GTNAC 4 cut(s) 481, 574, 832, 1027
MalI GATC 7 cut(s) 33, 163, 399, 1063, 1198, 1317, 1345
MboI GATC 7 cut(s) 31, 161, 397, 1061, 1196, 1315, 1343
MflI RGATCY 1 cut(s) 161
MluCI AATT 5 cut(s) 49, 381, 592, 753, 1151
MlyI GAGTC 3 cut(s) 13, 489, 1374
MroI TCCGGA 1 cut(s) 1257
MroXI GAANNNNTTC 1 cut(s) 876
MseI TTAA 4 cut(s) 48, 504, 1296, 1390
MslI CAYNNNNRTG 1 cut(s) 149
MspA1I CMGCKG 2 cut(s) 1158, 1321
MspI CCGG 4 cut(s) 520, 1128, 1258, 1304
MspR9I CCNGG 6 cut(s) 521, 704, 1128, 1275, 1304, 1305
Mva1269I GAATGC 1 cut(s) 743
MvaI CCWGG 2 cut(s) 704, 1275
MvnI CGCG 3 cut(s) 454, 644, 1321
MwoI GCNNNNNNNGC 1 cut(s) 1270
NciI CCSGG 4 cut(s) 521, 1128, 1304, 1305
NdeII GATC 7 cut(s) 31, 161, 397, 1061, 1196, 1315, 1343
NlaIII CATG 4 cut(s) 61, 253, 804, 845
NlaIV GGNNCC 3 cut(s) 75, 288, 1308
NmuCI GTSAC 2 cut(s) 481, 574
NspI RCATGY 1 cut(s) 804
PagI TCATGA 1 cut(s) 57
PctI GAATGC 1 cut(s) 743
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 3 cut(s) 331, 737, 1169
PkrI GCNGC 1 cut(s) 1323
PleI GAGTC 3 cut(s) 12, 488, 1373
PpsI GAGTC 3 cut(s) 12, 488, 1373
PpuMI RGGWCCY 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 74
Psp6I CCWGG 2 cut(s) 702, 1273
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 2 cut(s) 702, 1273
PspN4I GGNNCC 3 cut(s) 75, 288, 1308
PspPI GGNCC 3 cut(s) 74, 1277, 1307
PspPPI RGGWCCY 1 cut(s) 74
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 1 cut(s) 161
PvuII CAGCTG 1 cut(s) 1158
RsaI GTAC 2 cut(s) 200, 210
RsaNI GTAC 2 cut(s) 199, 209
RseI CAYNNNNRTG 1 cut(s) 149
SacII CCGCGG 1 cut(s) 1322
SaqAI TTAA 4 cut(s) 48, 504, 1296, 1390
SatI GCNGC 1 cut(s) 1322
Sau3AI GATC 7 cut(s) 31, 161, 397, 1061, 1196, 1315, 1343
Sau96I GGNCC 3 cut(s) 74, 1277, 1307
SchI GAGTC 3 cut(s) 13, 489, 1374
ScrFI CCNGG 6 cut(s) 521, 704, 1128, 1275, 1304, 1305
SfaNI GCATC 4 cut(s) 141, 434, 544, 1000
SfcI CTRYAG 2 cut(s) 188, 402
Sfr303I CCGCGG 1 cut(s) 1322
SgrBI CCGCGG 1 cut(s) 1322
SinI GGWCC 3 cut(s) 74, 1277, 1307
SmaI CCCGGG 1 cut(s) 1305
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 5 cut(s) 49, 381, 592, 753, 1151
SsiI CCGC 6 cut(s) 341, 644, 1135, 1271, 1319, 1321
SspMI CTAG 3 cut(s) 126, 651, 1058
StyD4I CCNGG 6 cut(s) 519, 702, 1126, 1273, 1302, 1303
TaaI ACNGT 6 cut(s) 178, 539, 722, 903, 1020, 1148
TaiI ACGT 2 cut(s) 488, 620
TaqI TCGA 4 cut(s) 26, 474, 1245, 1359
TasI AATT 5 cut(s) 49, 381, 592, 753, 1151
TatI WGTACW 2 cut(s) 198, 208
TauI GCSGC 1 cut(s) 1324
TfiI GAWTC 3 cut(s) 331, 737, 1169
Tru1I TTAA 4 cut(s) 48, 504, 1296, 1390
Tru9I TTAA 4 cut(s) 48, 504, 1296, 1390
TscAI CASTG 2 cut(s) 670, 754
TseFI GTSAC 2 cut(s) 481, 574
Tsp45I GTSAC 2 cut(s) 481, 574
TspGWI ACGGA 2 cut(s) 33, 747
TspMI CCCGGG 1 cut(s) 1303
TspRI CASTG 2 cut(s) 670, 754
VpaK11BI GGWCC 3 cut(s) 74, 1277, 1307
XagI CCTNNNNNAGG 1 cut(s) 405
XapI RAATTY 1 cut(s) 1151
XceI RCATGY 1 cut(s) 804
XmaI CCCGGG 1 cut(s) 1303
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 3 cut(s) 126, 651, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.